Polymorphisms in COL2A1 gene in Adolescents with Temporomandibular Disorders

2020 ◽  
Vol 44 (5) ◽  
pp. 364-372
Author(s):  
Bruna Cristina do Nascimento Rechia ◽  
Bruna Michels ◽  
Aluhe Lopes Faturri ◽  
Fernanda Mara de Paiva Bertoli ◽  
Rafaela Scariot ◽  
...  
Keyword(s):  
Genetic Polymorphisms ◽  
Myofascial Pain ◽  
Temporomandibular Disorder ◽  
Taqman Assay ◽  
Case Group ◽  
Unaffected Individual ◽  
Disc Displacement ◽  
Genotype Distribution ◽  
Col2a1 Gene ◽  
Chain Reactions

Objectives: Temporomandibular disorder (TMD) is considered a functional disorder with multifactorial aspects. The goal of this study was to investigate if genetic polymorphisms in the COL2A1 gene could be associated with TMD in adolescents. Study design: The case group (TMD-affected) included individuals diagnosed with any of the following TMD subgroups according to the RDC/TMD criteria: myofascial pain, disc displacements and arthralgia. Genomic DNA for molecular analysis was extracted from buccal cells and genetic polymorphisms in COL2A1 were genotyped by real time polymerase chain reactions using the TaqMan assay. Data were analyzed using the Epi Info 3.5.7 and Stata software. Results: 249 subjects were included in this study (148 subjects “affected” by TMD). There were no significant differences between the affected and unaffected individual (p>0.05), for TMD, arthralgia and myofascial pain however, rs2276454 was borderline in the genotype distribution (p=0.07) and was associated with disc displacement (p=0.03) in the allelic distribution. Recessive model showed significant differences between groups for with disc displacement (p=0.02). Conclusions: Genetic polymorphisms in COL2A1 are not associated with myofascial pain, arthralgia or TMD in adolescents but this study provides evidence that rs2276454 is involved in the disc displacement of the temporomandibular joint.

Five genetic polymorphisms of cytochrome P450 enzymes in the Czech non-Roma and Czech Roma population samples

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Lucie Dlouhá ◽  
Věra Adámková ◽  
Lenka Šedová ◽  
Věra Olišarová ◽  
Jaroslav A. Hubáček ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Genetic Polymorphisms ◽  
Metabolic Pathways ◽  
Cytochromes P450 ◽  
Taqman Assay ◽  
Cytochrome P450 Enzymes ◽  
Roma Population ◽  
Czech Population ◽  
Genotype Frequencies ◽  
Pcr Rflp

AbstractObjectivesCytochromes P450 play a role in human drugs metabolic pathways and their genes are among the most variable in humans. The aim of this study was to analyze genotype frequencies of five common polymorphisms of cytochromes P450 in Roma/Gypsy and Czech (non-Roma) population samples with Czech origin.MethodsRoma/Gypsy (n=302) and Czech subjects (n=298) were genotyped for CYP1A2 (rs762551), CYP2A6 (rs4105144), CYP2B6 (rs3745274) and CYP2D6 (rs3892097; rs1065852) polymorphisms using PCR-RFLP or Taqman assay.ResultsWe found significant allelic/genotype differences between ethnics in three genes. For rs3745274 polymorphism, there was increased frequency of T allele carriers in Roma in comparison with Czech population (53.1 vs. 43.7%; p=0.02). For rs4105144 (CYP2A6) there was higher frequency of T allele carriers in Roma in comparison with Czech population (68.7 vs. 49.8%; p<0.0001). For rs3892097 (CYP2D6) there was more carriers of the A allele between Roma in comparison with Czech population (39.2 vs. 38.2%; p=0.048). Genotype/allelic frequencies of CYP2D6 (rs1065852) and CYP1A2 (rs762551) variants did not significantly differ between the ethnics.ConclusionsThere were significant differences in allelic/genotype frequencies of some, but not all cytochromes P450 polymorphisms between the Czech Roma/Gypsies and Czech non-Roma subjects.

Sleep-associated aspects of myofascial pain in the orofacial area among Temporomandibular Disorder patients and controls

2015 ◽  
Vol 16 (9) ◽  
pp. 1056-1061 ◽  
Author(s):  
Marc Schmitter ◽  
Alexandra Kares-Vrincianu ◽  
Horst Kares ◽  
Justo Lorenzo Bermejo ◽  
Hans-Jürgen Schindler
Keyword(s):  
Myofascial Pain ◽  
Temporomandibular Disorder

scholarly journals The impact of ACE2 genetic polymorphisms (rs2106809 and rs2074192) on gender susceptibility to COVID-19 infection and recovery: A systematic review

2021 ◽  
Vol 2 (03) ◽  
pp. 166-179
Author(s):  
Ahmed Suleiman ◽  
Tamadher Rafaa ◽  
Ali Al­rawi ◽  
Mustafa Dawood
Keyword(s):  
Systematic Review ◽  
Genetic Polymorphisms ◽  
Disease Risk ◽  
Epidemiological Studies ◽  
Genotype Distribution ◽  
Male Mortality ◽  
Risk Genes ◽  
Angiotensin Converting Enzyme 2 ◽  
Susceptibility To Infection ◽  
The Impact

Background: Epidemiological studies revealed there is a difference in susceptibility to infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) because of differences in gender with age and males being more inflicted. There is a clear indication that deaths caused by coronavirus disease 2019 (COVID-19) in males appeared at a higher rate than females across 35 nations. The implication of associated disease-risk genes, involved in the susceptibility of COVID-19 such as the angiotensin-converting enzyme 2 (ACE2), has recently received considerable attention due to their role in severe injury of lung and mediated SARS-CoV-2 entry as a host receptor. Objectives: Herein, we aimed to systematically review how two main genetic polymorphisms of ACE2 (rs2106809 and rs2074192) can affect the gender susceptibility to SARS-CoV-2 infection. Methods: To conduct this systematic review, a literature search in PubMed, Google Scholar, ScienceDirect, and Nature was made for the period 2004 to 2020. We searched for the impact of ACE2 genetic polymorphisms (rs2106809 and rs2074192) on gender susceptibility. Results: We noticed that there was a differential genotype distribution between males and females in various global populations whereas mutant variants were common in males compared to wild-type variants among females, which may reflect differences in gender susceptibility to infection with SARS-CoV-2. Females are less susceptible to coronavirus as compare to males because of the expression of ACE2 receptor. It has a double role in favour of COVID-19 and against COVID-19. Conclusions: Male mortality is greater than female mortality, which might be attributed to the ACE2 deficiency in women. Epidemiological studies have shown that the differences in sex and age have different susceptibility to SARS-CoV-2 infection.

scholarly journals The importance of multisection sagittal and coronal magnetic resonance imaging evaluation in the assessment of temporomandibular joint disc position

2020 ◽  
Vol 25 (1) ◽  
pp. 159-168
Author(s):  
Monika Litko-Rola ◽  
Jacek Szkutnik ◽  
Ingrid Różyło-Kalinowska
Keyword(s):  
Sagittal Plane ◽  
Temporomandibular Disorder ◽  
False Negative ◽  
Disc Displacement ◽  
Resonance Imaging ◽  
Imaging Evaluation ◽  
Magnetic Resonance Imaging Evaluation ◽  
Temporomandibular Joint Disc ◽  
Disc Position ◽  
Tmj Disc

Abstract Objectives The aim of this study was to evaluate diagnoses of temporomandibular (TMJ) disc displacement by comparing evaluations done on the basis of central sagittal scans only, the most often used in temporomandibular disorder (TMD) patients, with a multisection evaluation done with both sagittal and coronal scans. Materials and methods Multisection MRI analysis of 382 TMJs was conducted in 191 patients with disc displacement according to RDC/TMD criteria. Disc position in the intercuspal position (IP) was assessed two times using two different methods. The first method involved a TMJ disc position evaluation on the central slide in the oblique sagittal plane only. In the second method, the TMJ disc position was assessed on all oblique sagittal and coronal images. McNemar’s χ2 test was used to evaluate the differences between the sensitivities of two methods. Results The first method (central oblique sagittal scans assessment) identified 148 TMJs (38.7%) with normal disc position compared with 89 TMJs (23.3%) with normal disc position found by the second method (all oblique sagittal and coronal scans assessment). The sensitivity of analysis in both planes was significantly higher than in the sagittal plane only (p < 0.001). Conclusions The multisection analysis in the sagittal and coronal plane allows to distinguish the correct disc position from disc displacement and thus improve evaluation of TMJ internal derangement. Clinical relevance The multisection sagittal and coronal images should be recommended as a standard in MRI of the TMJ disc displacement in patients with TMD to avoid false-negative diagnoses.

scholarly journals Determining Risk Factors for the Development of Temporomandibular Disorders during Orthodontic Treatment

2020 ◽  
Vol 10 (22) ◽  
pp. 8216
Author(s):  
Alessandro Ugolini ◽  
Federico Garbarino ◽  
Luca Di Vece ◽  
Francesca Silvestrini-Biavati ◽  
Valentina Lanteri
Keyword(s):  
Risk Factors ◽  
Temporomandibular Disorders ◽  
Orthodontic Treatment ◽  
Complex Disease ◽  
Myofascial Pain ◽  
Significant Risk ◽  
Female Gender ◽  
Structured Interview ◽  
Disc Displacement ◽  
One Year

Temporomandibular disorders (TMD) represent a complex disease with a multifactorial etiology. Despite several studies on the subject, a causal relationship between orthodontic treatment and different forms of TMD has not been established. The aim of this study was to analyze the effect of orthodontic treatment on two aspects of TMD: myofascial pain and disc displacement. This retrospective cohort study followed 224 orthodontic adult patients at three points in time: before treatment (T0), immediately after treatment (T1), and one year after treatment (T2). Disc displacement and myofascial pain were evaluated through a clinical assessment and with a semi-structured interview, along with headache, neck, and shoulder pain parameters and behavioral and somatic accompanying symptoms. Multivariate logistic regression was used to identify risk factors that could influence the development of TMD in these patients. There was a non-significant increase in disc displacement during orthodontic treatment, which mostly resolved after completion of treatment. Myofascial pain scores worsened during treatment, but improved when compared with the baseline once treatment was complete (T0 = 51.3%, T1 = 64.6%, T2 = 44.9%). Female gender (aOR = 1.9, CI 95%, 1.23–2.36), the presence of somatic symptoms (aOR = 3.6, CI 95%, 2.01–5.84), and symptoms of anxiety or depression (aOR = 2.2, CI 95%, 1.14–4.51) were significant risk factors associated with the development of TMD. There is a low and not significant risk of TMD development during orthodontic treatment. When TMD occurred, they resolved within 1 year of the end of treatment.

scholarly journals Self-medication Among Myofascial Pain Patients: A Preliminary Study

2018 ◽  
Vol 12 (1) ◽  
pp. 347-353 ◽  
Author(s):  
Gabriel Pires Pastore ◽  
Douglas Rangel Goulart ◽  
Patrícia Radaic Pastore ◽  
Alexandre Javaroni Prati ◽  
Márcio de Moraes
Keyword(s):  
Myofascial Pain ◽  
Temporomandibular Disorder ◽  
Correct Diagnosis ◽  
Self Medication ◽  
Correlative Analysis ◽  
Time Period ◽  
Severity Of The Disease ◽  
Level Of Significance ◽  
A Prospective Study ◽  
Pain Patients

Background: Self-medication has been reported as an option which people choose to relieve the suffering of conditions that cause pain, however, this could delay the correct diagnosis and therapy. Objective: The aim of the present study was to determine the prevalence of self-medication among patients with Temporomandibular Disorder (TMD), and to analyze correlations with the severity of the disease. Methods: A prospective study was conducted with patients who had been diagnosed with TMD. The patients were submitted to anamnesis and a physical examination. This research also used the Fonseca`s Anamnestic Index (FAI) and a questionnaire that was developed specifically for this study, containing questions related to the first health professional contacted and self-medication. The data were analyzed using comparative and correlative analysis (Version 18.0 of SPSS software), with the level of significance set at p<0.05. Results: Thirty-four patients were included, with a prevalence of females (91.2%) and a mean age of 39.76 years. Half of the patients claimed to have chosen their own medications at time, especially analgesics. Sodium dipyrone was used by 12 of the participants. Dentists were the most commonly contacted health professionals (55.5%). No correlation was found between self-medication and the severity of TMD according to the FAI. Furthermore, the time period between the onset of symptoms and the first consultation was not affected by self-medication. Conclusion: Self-medication seems to be highly prevalent among patients with TMD, although this practice does not seem to alter the severity of the disease.

scholarly journals The Application of Intra-Articulr Injections for Management of the Consequences of Disc Displacement without Reduction

2020 ◽  
Vol 17 (13) ◽  
pp. 4726
Author(s):  
Malgorzata Pihut ◽  
Andrzej Gala
Keyword(s):  
Hyaluronic Acid ◽  
Platelet Rich Plasma ◽  
Temporomandibular Disorder ◽  
Mean Value ◽  
Study Group ◽  
Disc Displacement ◽  
Clinical Parameters ◽  
Double Blind ◽  
Temporomandibular Joints ◽  
Functional Efficiency

The aim of the study was to make a comparative studies on the effectiveness of platelet rich plasma (PRP) and hyaluronic acid (HA) in intra-articular injections to the temporomandibular joints—in double blind studies application—based on the analysis of selected clinical parameters of functional efficiency and the mean value of joint’s pain intensity before and after management. The study enrolled a group of 100 patients, aged 21 to 43 years, of both sexes, who came for the prosthodontic treatment. All patients had II b group of disorder according to the Research Diagnostic Criteria/Temporomandibular Disorder, and were consecutively, alternately assigned to the groups, 50 patients in each. Study group PRP was treated with intra-articular injection of platelet rich plasma and study group HA had injection with hyaluronic acid. The examination was double-blind, so that the injecting physician and the patient were not informed what kind of medicinal substance they received in the joint injection. The final selected clinical parameters did not differ statistically significantly between the groups, what means that both administered substances were effective in the repair of intra-articular structures. The results of research showed that the use of PRP and HA in intraarticular joint’s injections positively affects in selected clinical parameters and decrease of the pain in temporomandibular joints in the case of disc displacement without reduction.

Association Between Genetic Polymorphisms in Metaloproteinases of the Matrix and Delayed Tooth Emergence: A Cross-sectional Study

2019 ◽  
Vol 10 (2) ◽  
pp. 91-96
Author(s):  
Silvane Silva Evangelista ◽  
Juliana Arid ◽  
Katia Regina Felizardo Vasconcelos ◽  
Giuseppe Valduga Cruz ◽  
André Luiz Tannus Dutra ◽  
...  
Keyword(s):  
Genetic Polymorphisms ◽  
Cross Sectional Study ◽  
Permanent Tooth ◽  
Tooth Eruption ◽  
Permanent Teeth ◽  
Fisher Exact Test ◽  
Genotype Distribution ◽  
Sectional Study ◽  
Cross Sectional ◽  
Allele Distribution

Background and Aims: Animal models have been demonstrating that MMPs have an important function in the tooth eruption process. The aim of this study was to evaluate the association between genetic polymorphisms in MMP8 and MMP13 and delayed tooth eruption of permanent teeth. Materials and Methods: This cross-sectional study selected 216 children, 9- to 12-year-old, from public schools at Manaus, Amazonas, Brazil. During oral clinical examination, each permanent tooth emerged in the oral cavity was evaluated. Children were considered with delayed tooth eruption when at least one permanent tooth was delayed and were classified in 2 groups: children “with delayed tooth emergency” and “without delayed tooth emergency.” Saliva samples were collected from DNA extraction. The genetic polymorphisms rs17099443 and rs3765620 in MMP8, and rs478927 and rs2252070 in MMP13 were genotyped. Statistical Analysis: PLINK V1.07 ( http://pngu.mgh.harvard.edu/purcell/plink/ ) and GraphPad Prism 5.0 (San Diego, CA, USA) were used. The c2 or Fisher exact test was used to calculate genotypes and alleles distributions. To compare the mean number of delayed teeth according to genotypes, the Kruskal-Wallis test with multiple comparison Dunn test was used. The established alpha for all comparisons was .05. Results: The polymorphism rs17099443 in MMP8 was associated with delayed tooth eruption in the genotype distribution ( P = .05). In the allele distribution, the C allele was underrepresented in children with delayed tooth eruption ( P = .01; OR = 0.61, 95% confidence interval, 0.41–0.9). Conclusion: The genetic polymorphism rs17099443 in MMP8 is associated with delayed tooth eruption.

scholarly journals Frequency of Temporomandibular Disorders in Asymptomatic Removable Partial and Complete Denture Wearers

2006 ◽  
Vol 3 (4) ◽  
pp. 291-296
Author(s):  
N. Dulčić ◽  
V. Jerolimov ◽  
J. Pandurić
Keyword(s):  
Temporomandibular Disorder ◽  
Complete Denture ◽  
Aetiological Factor ◽  
Disc Displacement ◽  
Removable Partial Denture ◽  
Partial Denture ◽  
Tissue Specific ◽  
Anterior Disc Displacement ◽  
Significant Difference ◽  
Long Time

A dogmatic view on occlusion as the main aetiological factor for temporomandibular disorder (TMD) has been present in the literature for a long time, but a direct scientific correlation between occlusal disorders and TMD has never been proven. The purpose of this study was to determine the frequency of TMD signs and tissue-specific diagnoses in a population of 164 asymptomatic participants, 70 removable partial denture wearers and 94 complete denture wearers of an average age of 61.3 years, by means of clinical manual functional analysis. TMD was found in 42.1% of the participants. No statistically significant difference in the occurrence of TMD was found between removable partial and complete denture wearers and between genders (P > 0.05). The most frequent tissue-specific diagnoses were osteoarthrosis (11%), total anterior disc displacement (9.1%) and partial anterolateral disc displacement (8.5%). The frequency of tissue-specific diagnoses was also not influenced by the type of prosthetic replacements.

scholarly journals Orthodontics is temporomandibular disorder–neutral

2015 ◽  
Vol 86 (4) ◽  
pp. 649-654 ◽  
Author(s):  
Daniele Manfredini ◽  
Edoardo Stellini ◽  
Antonio Gracco ◽  
Luca Lombardo ◽  
Luca Guarda Nardini ◽  
...  
Keyword(s):  
Temporomandibular Disorders ◽  
Joint Pain ◽  
Orthodontic Treatment ◽  
Normal Values ◽  
Temporomandibular Disorder ◽  
Control Group ◽  
Disc Displacement ◽  
History Of ◽  
Clinically Significant ◽  
Age And Sex

ABSTRACT Objectives:  To assess if subjects with a clinical diagnosis of temporomandibular disorders (TMDs) have a similar prevalence of orthodontic history as a population of TMD-free individuals and to assess if those subjects who have a history of ideal orthodontics have fewer symptoms than those with a history of nonideal orthodontics. Materials and Methods:  Two groups of age- and sex-matched individuals belonging to either a study (“TMD”) or a control group were recruited. Subjects who underwent orthodontic treatment were classified as having a history of ideal or nonideal orthodontics based on the current presence of normal values in five reference occlusal features. Results:  The correlation with a history of orthodontic treatment was not clinically significant for any of the TMD diagnoses (ie, muscle pain, joint pain, disc displacement, arthrosis), with Phi (Φ) coefficient values within the −0.120 to 0.058 range. Within the subset of patients with a history of orthodontics, the correlation of ideal or nonideal orthodontic treatment with TMD diagnoses was, in general, not clinically relevant or was weakly relevant. Conclusions:  Findings confirmed the substantial absence of clinically significant effects of orthodontics as far as TMD is concerned. The very low correlation values of a negative or positive history of ideal or nonideal orthodontics with the different TMD diagnoses suggest that orthodontic treatment could not have a true role for TMD.

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