Identification of Cultivable Microorganisms from Primary Teeth with Necrotic Pulps

2010 ◽  
Vol 34 (4) ◽  
pp. 329-333 ◽  
Author(s):  
Gildardo Ledezma-Rasillo ◽  
Hector Flores-Reyes ◽  
Ana Ma. Gonzalez-Amaro ◽  
Arturo Garrocho-Rangel ◽  
Ma. del Socorro Ruiz-Rodriguez ◽  
...  
Keyword(s):  
Root Canal ◽  
Primary Teeth ◽  
Bacterial Species ◽  
Anaerobic Bacteria ◽  
Sinus Tract ◽  
Operative Field ◽  
Root Canals ◽  
Carious Lesions ◽  
Oral Environment ◽  
Clostridium Spp

The objective of this study was to identify cultivable microorganisms from primary teeth with necrotic pulps. This experimental study included 21 patients of both sexes between 4 and 7 years of age with necrotic pulps in primary teeth. Twenty-one maxillary and mandibular molars containing at least 1 necrotic canal, an abscess or sinus tract, one or more radiolucent areas in the furcation or periapical region, teeth having at least two thirds of root length, and carious lesions directly exposed to the oral environment were included. After antisepsis of the oral cavity, anesthesia of the affected tooth, and isolation and disinfection of the operative field, 3 sterile absorbent paper points were sequentially placed for 30 seconds for the collection of samples. The samples were immediately processed in an anaerobic chamber, and all isolated microorganisms were identified. Anaerobic species (anaerobic facultative and moderate anaerobes) were isolated in all root canals; 68.4% of root canal samples studied showed a polymicrobial nature. Most of the isolate consisted of Bifidobacterium Spp2 and Streptococcus intermedius. Other less frequently encountered species were Actinomyces israelii, Bifidobacterium spp 1, Clostridium spp, and Candida albicans. Results indicate the existence of combinations of bacterial species in root canal infections of the primary dentition with necrotic pulps, anaerobic bacteria predominating.

The role of intracanal medicaments in inhibition of bacteria isolated from root canals of infected primary molars

2019 ◽  
Vol 14 (1) ◽  
pp. 92
Author(s):  
Dr. Maha Abdul- Kareem Mahmood ◽  
Dr. Huda Elias Ali ◽  
Dr. Haraa Khairi Abdul-Kadher
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Normal Saline ◽  
Root Canal ◽  
Primary Teeth ◽  
Primary Molars ◽  
Root Canals ◽  
Etiologic Agents ◽  
Micro Organisms

Microbes are considered as the primary etiologic agents in endodontic diseases.Disinfection of the root canal is obtained by the combined effect of biomechanicalpreparation, irrigation and intra canal medicament. The aim of the present study wasto assess the antimicrobial activity of intracanal medicaments (formocresol andEndosepton) against two micro organisms (Streptococcus mutans and staphylococcusaureus) isolated from 15 necrotic pulps of primary molars indicated for pulpectomyprocedure. The samples were cultured, and purified using microbiological evaluation.Broth dilution test was performed in our study by preparing test tubes containing10 ml of BHI broth (pH. 7) which then inoculated with strains of the tested bacteriaand incubated at 37 C° for 24 h. After over night incubaction, ten fold dilution weremade in test tubes containing 9 ml of normal saline by adding 1 ml of the inoculum tothe first tube . Then from dilution 10-1 , 0.1 ml of cell suspension was added to 9.9 mlof formocresol and endosepton, then 0.1 ml was taken and spread on duplicates ofBHI agar plates at different intervals and incubated aerobically for 24 h. at 37 C°.Colonies on the plates were counted after incubation and CFU/mL (colony formingunit) was calculated. Our results indicating that there were no significant differencesbetween the intracanal medicaments, but there were high significant differencesbetween the intervals time of the study. We concluded that both materials had greatantibacterial effect against the pathogens commonly isolated from necrotic pulpaltissue of primary teeth.

scholarly journals Inspection of the Microbiota in Endodontic Lesions

2019 ◽  
Vol 7 (2) ◽  
pp. 47 ◽  
Author(s):  
Mario Dioguardi ◽  
Giovanni Di Gioia ◽  
Gaetano Illuzzi ◽  
Claudia Arena ◽  
Vito Carlo Alberto Caponio ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Scientific Literature ◽  
Bacterial Species ◽  
Primary Objective ◽  
Endodontic Treatment ◽  
Canal System ◽  
Resistant Species ◽  
Carious Lesions ◽  
Root Canal System

The primary objective of endodontic therapy is to create a biologically acceptable environment within the root canal system that allows for the healing and maintenance of the health of the peri-radicular tissue. Bacteria are one of the main causes of pulp problems, and they have different methods of penetrating and invading the endodontic space such as through carious lesions, traumatic pulp exposures, and fractures. The types of bacteria found range from facultative anaerobes to aerobes, up to the most resistant species able to survive in nutrient-free environments; the bacterial species Enterococcus faecalis belongs to this last group. Enterococcus faecalis is considered one of the main causes of recurring apical periodontal lesions following endodontic treatment, with persistent lesions occurring even after re-treatment. The review presented in this paper was performed in accordance with the PRISMA protocol and covers articles from the related scientific literature that were sourced from PubMed, Scopus, and Google Scholar using the following terms as keywords: “endodontic treatment”, “endodontic bacteria”, “microbial endodontic”, and “endodontic failure”. Only the articles considered most relevant for the purposes of this paper were read in full and taken into consideration for the following review. The results show that Enterococcus faecalis, Actinomycetes, and Propionibacterium propionicum are the species most frequently involved in persistent radicular and extra-radicular infections.

Effect of Calcium Hydroxide as a Root Canal Dressing Material on Dentin Fracture Strength In Primary Teeth–In Vitro Study

2018 ◽  
Vol 42 (2) ◽  
pp. 146-149
Author(s):  
Fadi Said ◽  
Moti Moskovitz
Keyword(s):  
Calcium Hydroxide ◽  
Fracture Strength ◽  
Root Canal ◽  
Primary Teeth ◽  
In Vitro Study ◽  
Vitro Study ◽  
Root Canal Treatment ◽  
Root Canals ◽  
Anterior Teeth

Objectives: The aim of the present study was to assess the effect of calcium hydroxide as a root canal dressing material on dentin microtensile fracture strength in human primary teeth in vitro. Study design: Thirty primary anterior teeth with root canals packed with calcium hydroxide were divided into groups of ten and immersed in saline at room temperature for 7, 30 and 90 days. Ten teeth with root canals filled with sterile saline were the control group. Microtensile fracture strength was measured in Mechanical tester Lloyd testing machine. Results: There was a significant difference (P < 0.05) between the fracture strength of the calcium hydroxide-filled teeth after 90 days (19.1 MPa) compared with the control (35.8 MPa). Dentin microtensile fracture strength of the calcium hydroxide-filled teeth decreased at an average of 0.142 MPa per day. Conclusion: Calcium hydroxide placed in root canals for an extended time had a significantly negative effect on root strength. Long-term success of root canal treatment in primary anterior teeth is estimated as 65% with most of the failures result from trauma recurrence. Clinical Relevance: Our results stress the need to evaluate the pros and cons of root canal treatment compared to extractions of non-vital primary incisors.

scholarly journals A review of microbiological root canal sampling: updating an emerging picture

2013 ◽  
Vol 9 (1) ◽  
Author(s):  
Ana Moura Teles ◽  
M. Conceição Manso ◽  
Cristina Pina ◽  
José Cabeda
Keyword(s):  
Root Canal ◽  
English Language ◽  
Anaerobic Bacteria ◽  
Sample Collection ◽  
Scientific Rigor ◽  
Root Canals ◽  
Additional Information ◽  
Endodontic Infections ◽  
Language Accessibility ◽  
Positive Results

Objectives: The significance of microorganisms in root canals with regard to the aetiology of periapical infection and the need for crucial bacteria control during treatment are undeniable. In this study, we re-port and discuss a review of the literature on Microbiological Root Canal Sampling (MRS). The procedure is analyzed in detail, discussing its powers, limitations and the influence of sample collection procedures on the incidence of true and false positive results. Data sources: MEDLINE/PUBMED, B-On and library files of Oporto University were accessed. Selection: Papers were selected using the keywords: “root ca¬nal sampling”; “apical periodontitis”; “endodontic pathogens”; “root canal infection”; “Culture”; “molecular biology”. The references were selected under inclusion criteria such as English language, accessibility, rel¬evance to the theme and scientific rigor. Conclusions: This review illustrated the absolute need to adhere to strict methodology procedures if valid samples are to be obtained. A combination of Culture and molecular identification approaches have confirmed the polymicrobial nature of endodontic infections with a pre¬dominance of anaerobic bacteria. Nucleic acid-based techniques provide significant additional information particularly regarding the not-yet-cultivable species of the microbial community, but greatly increase the budget of the procedure. Thus, assessment of the endodontic microflora, in the context of a polymicro¬bial biofilm ecosystem, and its relevance to endodontic treatments must rely in the complementariness of Culture and Metagenomics approaches as they are neither mutually exclusive nor competitive, but strongly complementary.

scholarly journals Determining of the Most Optimal Parameters of Monopolar High Frequency Diathermocoagulation in Endodontic Practice

2021 ◽  
Vol 36 (1) ◽  
pp. 252-256
Author(s):  
Daurova Fatima Yuryevna ◽  
Tomaeva Diana Islanbekovna ◽  
Gasanova Zarema Magomedovna ◽  
Butaeva Natalia Taymurazovna ◽  
Podkopaeva Svetlana Vasilevna ◽  
...  
Keyword(s):  
Root Canal ◽  
High Frequency ◽  
Antibacterial Effect ◽  
Dental Treatment ◽  
Anaerobic Bacteria ◽  
Thermal Imager ◽  
Tooth Root ◽  
Optimal Parameters ◽  
Root Canals ◽  
The Third

The main criterion for successful endodontic treatment is thorough cleaning of the root canal from microorganisms. Modern technologies are being developed to decontaminate the root canal system due to local temperature increase. High-frequency monopolar diathermocoagulation refers to one of these technologies. Aim. To determine the optimal parameters of monopolar high-frequency diathermocoagulation providing a distinct antibacterial effect and which are safe for the surrounding tissues in endodontic dental treatment. Materials and methods. Root canals of single-root removed teeth from the group of upper and lower jaw incisors (12 samples). The DC-35 MS device (2640 kHz, 1. 5 kOhm) was used to study various modes of high-frequency monopolar diathermocoagulation. 6 exposure modes were studied. To determine the heating degree of the tooth root surface, thermometry using a compact infrared radiation converter of the Seek Thermal imager was used, and a remote infrared thermometer Testo 830-T1 was used for temperature monitoring. After determining of the diathermocoagulation modes that are not able to cause significant heating of the surrounding tooth tissues, the antibacterial effect of diathermocoagulation in these modes was studied. To do this, we used clinical strains of optionally anaerobic bacteria obtained from the teeth root canals in pulpitis: Streptococcus sanguis, Streptococcus mutans, Enterococcus faecalis, Escherichia coli and yeast-like fungi Candida albicans. Results. When performing diathermocoagulation of the root canal contents, the third, fourth and sixth modes of the device settings with the power given to the tissue within three seconds of energy in the range of 3.06 W to 5.4 W are safe for the tissues surrounding the tooth root. When treating root canals in the third and sixth modes, the power of diathermocoagulation is 5.4 W and 4.1 W. A multiple, significant decrease in microbial contamination of root canals by all kinds of pathogenic optional anaerobic microflora obtained from the teeth root canals in pulpitis was found.

scholarly journals Identifikasi bakteri pada saluran akar gigi dengan diagnosis periodontitis apikalis kronis (Identification of bacteria in dental root canal diagnosed with chronic apicalis periodontitis)

2014 ◽  
Vol 13 (3) ◽  
pp. 182
Author(s):  
Yeyen Sutasmi ◽  
Nurhayati Natsir
Keyword(s):  
Root Canal ◽  
Anaerobic Bacteria ◽  
Apical Periodontitis ◽  
Root Canals ◽  
Different Types ◽  
Dental Hospital ◽  
Conservative Dentistry ◽  
Periapical Diseases ◽  
Identification Of Bacteria ◽  
Dominant Bacteria

Chronic apical periodontitis is one of common periapical diseases. Inflammation in the periapical tissue is caused bybacteria from the root canal necrotic. If the tooth is not treated, the infection can progress to periapical region causingchronic apical periodontitis. The variety of bacteria can be found in root canal with chronic apical periodontitis. Thisstudy aims to determine the type of bacteria in chronic apical periodontitis. Samples were obtained from fifteen patientswith teeth that have chronic apical periodontitis in the Department of Conservative Dentistry in Dental Hospital ofHasanuddin University. Bacteria collected from a sample specimen using paper points which were inserted into the rootcanal. Subsequent, bacterial specimens identified in the Medical Microbiology Laboratory of the Medical Faculty,Hasanuddin University. This study found seven different types of bacteria with varying percentages. Type of Grampositivefacultative anaerobic bacteria Streptococcus spp. is the most dominant bacteria found in the root canal withchronic apical periodontitis. This study concluded that the dominant bacteria in the root canals with chronic apical periodontitis were Streptococcus spp.

scholarly journals Patterns of Oral Microbiota in Patients with Apical Periodontitis

2021 ◽  
Vol 10 (12) ◽  
pp. 2707
Author(s):  
Izabela Korona-Glowniak ◽  
Dominika Piatek ◽  
Emilia Fornal ◽  
Anna Lukowiak ◽  
Yuriy Gerasymchuk ◽  
...  
Keyword(s):  
Root Canal ◽  
Secondary Infection ◽  
Bacterial Species ◽  
Keystone Species ◽  
Pcr Amplification ◽  
Permanent Teeth ◽  
Rrna Gene ◽  
Oral Microbiota ◽  
Root Canals ◽  
Periapical Periodontitis

In this study, microbial diversity of the root canal microbiota related to different endodontic infections was investigated. In total, 45 patients with endo–perio lesions (8 patients), chronic periapical periodontitis (29 patients) and pulp necrosis (8 patients) were recruited. In 19 (42.2%) patients there was secondary infection of root canals. Microbial specimens were collected from root canals of non-vital teeth with or without changes in periapical area visible in X-ray. Then, oral microbiota were detected and identified using the culture method and real-time PCR amplification primers and hydrolysis-probe detection with the 16S rRNA gene as the target. Overall, 1434 species/genes from 41 different genera of 90 various microbial species were retrieved. Of the major reported phyla, Firmicutes (62.9%), Actinobacteria (14.0%), Bacteroidetes (12.1%), Proteobacteria (9.1%) and Fusobacteria (4.2%) were detected. Of the bacterial species, 54.6% were strict anaerobes. Corynebacterium matruchotii (p = 0.039) was present significantly more frequently in chronic periapical periodontitis. Moreover, the higher values of Decayed, Missing and Filled Permanent Teeth index were positively correlated with relative abundance of Actinomyces spp. (p = 0.042), Lactobacillus spp. (p = 0.006), Propionibacterium spp. (p = 0.024) and Rothia spp. (p = 0.002). The multivariate analyses revealed differences in total root canal samples, where components that affected grouping of root samples into four main categories were identified. Anaerobic Gram-negative bacteria predominated in root canals of teeth with pulp necrosis and periapical lesions. Facultative anaerobic Gram-positive bacteria predominated in canals with secondary infections. All detected members of mixed population groups that might serve as keystone species contributed to the entire community in its clinical relevance.

scholarly journals Comparison of root canal length measurement methods in primary teeth

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Mine Koruyucu ◽  
Merve Bayram ◽  
Yelda Kasımoğlu ◽  
Figen Seymen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Root Canal ◽  
Primary Teeth ◽  
Conventional Radiography ◽  
Actual Length ◽  
Root Canals ◽  
Working Length ◽  
Electronic Apex Locator ◽  
Scanning Electron

Objectives: The purpose of this study was to evaluate the accuracy of conventional radiography, intraoral digital radiovisiography and electronic apex locator in determining the working length of root canals in primary teeth (in-vivo) and to compare the results with scanning electron microscopy measurements (ex-vivo). Materials and Methods: This study was conducted on 50 primary molar teeth. Standard endodontic access cavity preparations were prepared and the actual length was calculated by calibrated investigators. Working lengths were determined by using conventional radiography, RVG and apex locator in a total of 116 root canals. After the extraction of the teeth, each canal length was determined by using SEM. Data were analysed statistically by using Oneway Anova, Tukey HDS, Student t test and Bonferroni tests. Results: The mean root length measurements with conventional radiography were significantly higher than apex locator, RVG and SEM. The accuracy of apex locator and RVG were higher than conventional radiography in determining the working length in primary teeth. Conclusions: The electronic apex locators provide an acceptable level of accuracy in determining root canal length in primary teeth. Clinical Relevance: The purpose of this study was to evaluate the accuracy of conventional radiography, intraoral digital radiovisiography and electronic apex locator in determining the working length of root canals in primary teeth and,to compare the results with scanning electron microscopy measurements.

scholarly journals Surgical Sealing of Laterally Localized Accessory Root Canal with Resin Containing S-PRG Filler in Combination with Non-Surgical Endodontic Treatment: A Case Report

2020 ◽  
Vol 8 (4) ◽  
pp. 131
Author(s):  
Shizu Hirata-Tsuchiya ◽  
Shigeki Suzuki ◽  
Takashi Nakamoto ◽  
Naoya Kakimoto ◽  
Satoru Yamada ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Root Canal ◽  
Alveolar Bone ◽  
Clinical Symptoms ◽  
Adhesive System ◽  
Sinus Tract ◽  
Endodontic Treatment ◽  
Periodontal Tissue ◽  
Good Clinical Outcome ◽  
Root Canals

The spread of root canal infection to surrounding periodontal tissue through accessory root canals reduces the success rate of endodontic treatment. In this case, cone-beam computed tomography revealed a lesion (4 mm from the apex) resulting from an accessory root canal of the maxillary left central incisor. First, non-surgical endodontic treatment was conducted but the sinus tract remained. Surgical preparation of the root cavity was then conducted to remove potentially infected dentin surrounding the accessory root canal. The cavity was filled and the foramen was sealed with resin containing bioactive surface pre-reacted glass (S-PRG) filler. The photopolymerized resin was then contoured and polished. In combination with subsequent supportive non-surgical endodontic treatment, a good clinical outcome with the disappearance of the sinus tract and clinical symptoms such as discomfort and pressure pain and the regeneration of the alveolar bone hanging over the cavity was obtained. In this case, the good clinical outcome may have been due to the dentin-adhesive property and durability of the pre-adhesive system and composite resin. The better biocompatibility of S-PRG fillers presumably facilitated periodontal tissue healing.

scholarly journals Microbial Profile and Endotoxin Levels in Primary Periodontal Lesions with Secondary Endodontic Involvement

2019 ◽  
Vol 30 (4) ◽  
pp. 356-362
Author(s):  
Emanuel da Silva Rovai ◽  
Felipe de Souza Matos ◽  
Warley David Kerbauy ◽  
Flávia Goulart da Rosa Cardoso ◽  
Frederico Canato Martinho ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Root Canal ◽  
Bacterial Species ◽  
Wilcoxon Test ◽  
Periodontal Pocket ◽  
Strongly Correlated ◽  
Exact Test ◽  
Root Canals ◽  
Microbial Profile ◽  
Lal Assay

Abstract This study was carried out to investigate the microbial profile and endotoxin levels of endodontic-periodontal lesions of periodontal origin. Periodontal and endodontic samples were taken from periodontal pockets and necrotic root canals of 10 teeth with endodontic-periodontal lesions. Evidencing of 40 different bacterial species were determined in each endodontic and periodontal sample using the checkerboard DNA-DNA hybridization method and Kinetic chromogenic LAL assay was used for quantification of endotoxins. Fisher’s exact test correlated the bacterial species with the endodontic or periodontal microbiota. The endotoxin levels (EU/mL) found in samples of the root canal and periodontal pocket were compared by the Wilcoxon test (p<0.05). Bacteria and LPS units were found in 100% of the endodontic and periodontal samples. The species E. faecium, P. acnes, G. morbillorum, C. sputigena and L. buccalis were strongly correlated with the endodontic microbiota and P. nigrescens with the periodontal microbiota. P. intermedia, P. endodontalis and V. parvula were more prevalent in both endodontic and periodontal microbiots. The endotoxin levels in the periodontal pocket (89600 EU/mL) were significantly higher than in the root canal (2310 EU/mL). It was concluded that the microbiota present in the periodontal and endodontic tissues is similar, with a higher prevalence of species of the orange complex and a higher level of endotoxin in the periodontal pockets.

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