scholarly journals PROCALCITONIN OF EYACULATE AS A MARKER OF DISORDERS OF SPERMATOGENESIS

2019 ◽  
Vol 21 (1) ◽  
pp. 132-136
Author(s):  
K R Gal’kovich ◽  
D Yu Sosnin ◽  
O Yu Nenasheva ◽  
A V Krivcov
Keyword(s):  
Blood Serum ◽  
Seminal Plasma ◽  
Comparison Group ◽  
Sperm Concentration ◽  
Protein Composition ◽  
Total Content ◽  
Cellular Composition ◽  
Local Production ◽  
Procalcitonin Level

The study of the protein composition of the ejaculate is devoted to a significantly smaller number of studies than the characterization of the cellular composition of sperm and the characterization of spermatozoa. Objective: to investigate the concentration of procalcitonin (PCT) in seminal plasma and serum in normal conditions and with oligozoospermia. Materials and methods. 88 men were examined (the main group - 40 men with oligozoostantenospermia, the comparison group - 48 men with a normal concentration of spermatozoa). Results and discussion. The concentration of PCT in seminal plasma in the examined patients (n = 88) was almost 10 times higher than its serum content and amounted to 0.349 ± 0.370 ng/ml versus 0.037 ± 0.027 ng/ml (p0.000001). In patients with oligozoospermia the contents of the PC has exceeded the value of the comparison group (p = 0,0095). Serum PCT concentrations in blood serum did not differ between groups (p = 0.605). There were no statistically significant correlations between the level of PCT and other indicators of sperm (sperm concentration, their total content and volume of ejaculate). Summary. The content of PCT in seminal plasma does not depend on its level in blood serum. A higher content of PCT in the ejaculate compared to serum indicates its local production in the reproductive system. The increased procalcitonin level in seminal plasma may indicate decreased fertility of the ejaculate.

scholarly journals C-reactive protein and procalcitonin in biological liquids in men with normal and reduced fertility

2020 ◽  
pp. 68-71
Author(s):  
D. Yu. Sosnin ◽  
K. R. Galkovich ◽  
A. V. Krivtsov
Keyword(s):  
Blood Serum ◽  
Seminal Plasma ◽  
Comparison Group ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sperm Cells ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Biological Liquids ◽  
And Control ◽  
Experimental Group

Objective: A comparative analysis of C-reactive protein (CRP) and procalcitonin concentration in blood serum and seminal plasma of healthy men and men with reduced concentration of sperm cells.Methods: 73 fertile men were examined. The experimental group included 36 patients with a reduced fertility; the comparison group (control) included 37 men with normal indicators of ejaculate composition. The concentrations of CRP and procalcitonin were determined by the method of enzyme-linked immunosorbent assay.Results: Differences in level of CRP between blood serum and seminal plasma in experimental and control groups occurred to be statistically significant in both groups. There was no correlation between these parameters. The concentration of procalcitonin was almost ten times higher in the seminal plasma than in the blood serum. In the experimen‑ tal group, it was significantly higher in ejaculate than in the comparison group. The concentration of CRP and procalcitonin did not correlate neither with the ejaculate volume nor with concentration or amount of sperm cells in it.Conclusions: The levels of CRP and procalcitonin in seminal plasma can indicate the fertility status of the ejaculate. 

scholarly journals Relationship between abnormal spermatozoa and seminal plasma free amino acids in boars

2008 ◽  
Vol 52 (No. 2) ◽  
pp. 44-49 ◽  
Author(s):  
J. Čeřovský ◽  
S. Frydrychová ◽  
A. Lustyková ◽  
M. Rozkot
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Seminal Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Sperm Concentration ◽  
Negative Relationship ◽  
Total Content ◽  
Group A ◽  
Plasma Free Amino Acids

The objective of this study was to provide some data on concentrations of seminal plasma free amino acids (FAAs) in order to compare these values with different total contents of morphologically abnormal spermatozoa (MAS) in the semen of 37 experimental adult boars. The boars were kept in the same housing, feeding and breeding conditions. Immediately after collection, semen gel free volume, sperm concentration and total MAS were determined microscopically. The boars were divided into two groups (A &minus; <i>n</i> = 24, B &minus; <i>n</i> = 13) according to the significantly different total MAS content (<i>x</i>: A = 9.46 &plusmn; 4.95, B = 46.00 &plusmn; 14.54%, <i>P</i> < 0.01). Deproteinized seminal plasma samples (supernatant) were analysed for concentrations of 13 FAAs: Tau, Asp, Thr, Ser, Glu, Gly, Ala, Val, Met, Ile, Leu, Tyr and Lys by liquid chromatography (AAA 339 M analyser). A highly significant correlation was determined between total MAS and total FAA content in all experimental boars (<i>n</i> = 37, <i>r</i> = &minus;0.60, <i>P</i> < 0.01). Glutamic acid was a predominant FAA in seminal plasma in both groups of boars (x: A = 28.49 vs. B = 17.60&mu;M/100 ml) but its concentration was significantly higher in group A (<i>P</i> < 0.01). The proportion (%) of glutamic acid concentration in the total content of FAAs in both groups of boars was nearly equal (A = 38.1 vs. B = 38.9%, <i>P</i> > 0.05) as well as the proportion index of the individual FAAs calculated from glutamic acid (= 100%). The differences in the particular FAAs recorded between group A and group B were statistically significant for 11 out of the 13 FAAs observed (<i>P</i> < 0.05 and <i>P</i> < 0.01) in favour of group A as well as in the total calculated content of FAAs per boar (x: 74.70 vs. 45.23&mu;M/100 ml, <i>P</i> < 0.01). A significantly negative relationship between the MAS content and the concentration of seminal plasma FAAs (<i>r</i> = &minus;0.60, <i>P</i> < 0.01) is the main result of this study with regard to the markers for potential boar semen fertility estimation.

The effects of Pb on sperm parameters and sperm DNA fragmentation of men investigated for infertility

2020 ◽  
Vol 31 (4) ◽  
Author(s):  
G.U.S. Wijesekara ◽  
D.M.S. Fernando ◽  
S. Wijeratne
Keyword(s):  
Dna Fragmentation ◽  
Seminal Plasma ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
World Health ◽  
Sperm Dna Fragmentation ◽  
Progressive Motility ◽  
Sperm Dna ◽  
The Mean ◽  
Pb Concentration

AbstractBackgroundLead (Pb) is one of the metals most prevalent in the environment and is known to cause infertility and deoxyribonucleic acid (DNA) fragmentation. This study aimed to determine the association between seminal plasma Pb and sperm DNA fragmentation in men investigated for infertility.MethodsMale partners (n = 300) of couples investigated for infertility were recruited after informed consent was obtained. Sperm parameters were assessed according to the World Health Organization (WHO) guidelines. Seminal plasma Pb was estimated by atomic absorption spectrophotometry after digestion with nitric acid.ResultsIn Pb-positive and -negative groups the sperm parameters and sperm DNA fragmentation were compared using independent sample t-test and the Mann-Whitney U-test, respectively. The mean [standard deviation (SD)] age and duration of infertility were 34.8 (5.34) years and 45.7 (35.09) months, respectively, and the mean Pb concentration was 15.7 μg/dL. In Pb positives compared to Pb negatives the means (SD) of sperm count, progressive motility viability and normal morphology were lower (p > 0.05) but the DNA fragmentation was significantly higher 39.80% (25.08) than Pb negatives 22.65% (11.30). Seminal plasma Pb concentration and sperm DNA fragmentation had a positive correlation (r = 0.38, p = 0.03). A negative correlation was observed between sperm DNA fragmentation and sperm concentration, progressive motility, total motility and viability. When the DNA fragmentation was ≥30% sperm concentration and viability decreased (p < 0.05).ConclusionsPb in seminal plasma had a significant effect on sperm DNA fragmentation but not with other sperm parameters.

scholarly journals Analysis of the RNA content of the exosomes derived from blood serum and urine and its potential as biomarkers

2014 ◽  
Vol 369 (1652) ◽  
pp. 20130502 ◽  
Author(s):  
Mu Li ◽  
Emily Zeringer ◽  
Timothy Barta ◽  
Jeoffrey Schageman ◽  
Angie Cheng ◽  
...  
Keyword(s):  
Blood Serum ◽  
Cell Communication ◽  
Cell Culture Model ◽  
Culture Model ◽  
Wide Range ◽  
Abnormal Cells ◽  
A Cell ◽  
Invasive Diagnostics ◽  
Serum And Urine

Exosomes are tiny vesicles (30–150 nm) constantly secreted by all healthy and abnormal cells, and found in abundance in all body fluids. These vesicles, loaded with unique RNA and protein cargo, have a wide range of biological functions, including cell-to-cell communication and signalling. As such, exosomes hold tremendous potential as biomarkers and could lead to the development of minimally invasive diagnostics and next generation therapies within the next few years. Here, we describe the strategies for isolation of exosomes from human blood serum and urine, characterization of their RNA cargo by sequencing, and present the initial data on exosome labelling and uptake tracing in a cell culture model. The value of exosomes for clinical applications is discussed with an emphasis on their potential for diagnosing and treating neurodegenerative diseases and brain cancer.

Characterization of a Spray-Dried Soymilk Powder and Changes Observed During Storage

2010 ◽  
Vol 16 (2) ◽  
pp. 169-178 ◽  
Author(s):  
G. Osthoff ◽  
A. Hugo ◽  
P. van Wyk ◽  
M. de Wit ◽  
S. Meyer
Keyword(s):  
Unsaturated Fatty Acids ◽  
Storage Temperature ◽  
Chemical Properties ◽  
Chemical Characterization ◽  
Protein Composition ◽  
Absorption Spectrometry ◽  
Proximate Analysis ◽  
Powder Particles ◽  
High Level

Physical characterization of a soymilk powder was carried out by electron microscopy. Chemical characterization was analyzed by proximate analysis, mineral composition by atomic absorption spectrometry, fatty acid composition by gas chromatography and protein composition by electrophoresis. The powder consists of large granules of 60—80 μm, which may be hollow, with smaller granules of 10—20 μm attached to them. Powder particles are covered by a layer of fat. During storage at 25 °C fat is spreading over the surface, while at —12 °C the fat is contracting. This change affected chemical stability, resulting in high level of fat oxidation when stored at 4 °C or 25 °C as well as a decrease in unsaturated fatty acids. Storage also affected the chemical properties of the re-constituted soymilk; the pH of a 12% soy powder suspension increased from 6.68±0.05 to 7.06±0.08 after 12 months of storage. Storage temperature did not affect the pH of the suspension and this change could also not be ascribed to protein aggregation.

Initial Characterization of the Human Seminal Plasma Proteome in 3 Fertile Normogonadic Men by Top-Down Strategy.

2010 ◽  
pp. P3-342-P3-342
Author(s):  
D. Milardi ◽  
G. Grande ◽  
F. Vincenzoni ◽  
A. Giampietro ◽  
A. Bianchi ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteome ◽  
Human Seminal Plasma ◽  
Top Down ◽  
Initial Characterization

scholarly journals Weathering features of a remineralizer in soil under different land uses

2021 ◽  
Vol 56 ◽  
Author(s):  
Rafael Cipriano da Silva ◽  
Edilene Pereira Ferreira ◽  
Antonio Carlos de Azevedo
Keyword(s):  
Amorphous Phase ◽  
Ammonium Oxalate ◽  
Total Content ◽  
Sodium Dithionite ◽  
Land Uses ◽  
Elephant Grass ◽  
X Ray ◽  
Time Periods ◽  
Gains And Losses

Abstract The objective of this work was to analyze the mineralogical, morphological, and compositional modifications resulting from the weathering of diabase grains buried into soil under different land uses for up to 378 days. Samples of comminuted diabase were put into polyester bags and buried into soil under corn crop, elephant grass, and woods, being unburied and evaluated after four time periods. The samples of the remineralizer (RM) were analyzed by X-ray diffractometry, total chemical analysis, scanning electron microscopy, and Al (Ald and Alo) and Fe (Fed and Feo) contents extracted by sodium dithionite-citrate-bicarbonate (DCB) and ammonium oxalate (AAO) solutions. Plagioclases and pyroxenes were the most weathered minerals in all three land uses and showed the same pattern of elemental gains and losses. The characterization of Fe and Al solubility in DCB and AAO showed that the greatest change in these elements was from the lithogenic and crystalline to the pedogenic and amorphous phase, when compared with their total content. Plagioclases and pyroxenes were the most weathered minerals, and Fe and Al show a great transfer from the crystalline to the amorphous phase, with values up to 26 and 175, respectively, for the ratios between bags with RM/Feo and RM-control and bags with RM/Alo and RM-control.

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