scholarly journals Antioxidant and Atibacterial Activities of Nipah (Nypa fruticans) against Vibrio sp. Isolated From Mud Crab (Scylla sp.)

2017 ◽  
Vol 19 (3) ◽  
pp. 241
Author(s):  
Imra Imra ◽  
Kustiariyah Tarman ◽  
Desniar Desniar
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Crude Extract ◽  
Active Compound ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Mud Crab ◽  
Fruit Extract ◽  
Nypa Fruticans ◽  
Dpph Method

<p>Abstract<br />Nipah (Nypa fruticans) is the potential plant for source of active compound such as antioksidant and antibacterial substances. The plants are dispersed in Sumatera, Kalimantan, Sulawesi, Maluku and Papua Island. The aim of this research were determine the antioxidant and antibacterial activity from of nipah (fruit and leaf) that it extraction with methanol, and than determine toxicity and active compound contained in this extract. Diffusion agar and DPPH method were use for antibacterial and antioxsidant assay, respectively. Antioxsidant activity from nipah’s leaf extract was more effective (22,5 μg/mL) than nipah’s fruit extract (415 μg/mL). This activity to be classified to the strong antioxidant activity (IC50&lt;50 μg/mL). The antibacterial activity from leaf extract was strong to inhibited Vibrio sp. with inhibition zone 8,75 mm. The crude extract of nipah’s leaf was toxic with toxicity value is 663,598 μg/mL. Flavonoids, steroids, tanin, saponin and phenol hidroquinon were the active compounds contained in the extract of nipah’s leaf.</p>

scholarly journals Antioxidant and Atibacterial Activities of Nipah (Nypa fruticans) against Vibrio sp. Isolated From Mud Crab (Scylla sp.)

2016 ◽  
Vol 19 (3) ◽  
pp. 241 ◽  
Author(s):  
Imra Imra ◽  
Kustiariyah Tarman ◽  
Desniar Desniar
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Crude Extract ◽  
Active Compound ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Mud Crab ◽  
Fruit Extract ◽  
Nypa Fruticans ◽  
Dpph Method

Nipah (Nypa fruticans) is the potential plant for source of active compound such as antioksidant and antibacterial substances. The plants are dispersed in Sumatera, Kalimantan, Sulawesi, Maluku and Papua Island. The aim of this research were determine the antioxidant and antibacterial activity from of nipah (fruit and leaf) that it extraction with methanol, and than determine toxicity and active compound contained in this extract. Diffusion agar and DPPH method were use for antibacterial and antioxsidant assay, respectively. Antioxsidant activity from nipah’s leaf extract was more effective (22,5 µg/mL) than nipah’s fruit extract (415 µg/mL). This activity to be classified to the strong antioxidant activity (IC50&lt;50 µg/mL). The antibacterial activity from leaf extract was strong to inhibited Vibrio sp. with inhibition zone 8,75 mm. The crude extract of nipah’s leaf was toxic with toxicity value is 663,598 µg/mL. Flavonoids, steroids, tanin, saponin and phenol hidroquinon were the active compounds contained in the extract of nipah’s leaf.

Development of Gel Dossage Form From Mulberry Fruit Extract as A Facial Treatment

2018 ◽  
Vol 9 (01) ◽  
Author(s):  
Dhanesh Arif ◽  
Diah Lia Aulifa ◽  
Driyanti Rahayu ◽  
Arni Praditasari
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Vitamin C ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Microdilution Method ◽  
Mulberry Fruit ◽  
Ic50 Value ◽  
Dpph Method

Skin care is very important in cosmetics, especially facial treatments. Black mulberry is rich in phenols and is therefor usable in the treatment of acne. It also contains anthocyanin, a well-known antioxidant and a potential source of sun protection. This research aimed to develop a gel dossage from black mulberries (Morus nigra) extracts that would function as an antibacterial, antioxidant and sun blocking facial treatment. This research started with black mulberry fruit extracted through the maceration method by using ethanol (96%). Then, the antibacterial activity of the extract was determined by the disc-diffusion method, while the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by the microdilution method. Antioxidant activity of the extract was determined using the 1,1- diphenyl-2-picrilhidrazil (DPPH) method with vitamin C as a comparison. Furthermore, the extracts were formulated into gel formulas with variations of HPMC, Na-CMC, carbopol 934 and extract concentrations. The products were then physically evaluated for organoleptics, homogeneity, pH, viscosity, and dispersion as well as undergoing a hedonic test, an irritation test, an antioxidant activity test and determining the SPF value of the preparation. The results showed that the black mulberry fruit extract has antibacterial activity with MIC value of 0,025 g/mL against S. epidermidis and P. acnes, while MBC values were 0,025 g/mL and 0,05 g/mL, respectively. The black mulberry extract had antioxidant activity with IC50 value, i.e 146,73 μg/mL vitamin c i.e. 3,17 μg/mL. Formulation with best physical evaluation results showed ina formula containing a carbopol gel base of 934 0,015 g/mL with an extract cconcentration of 0,075 g/mL. This formula resulted in antioxidant activity with an IC50 value, i.e 1004,6 μg/mL, antibacterial activity with inhibition zone 6.83 ± 1.4 mm against and 6.76 ± 0.9 mm against S. epidermidis and P. acnes respectively and an SPF value of 1.9.

Antioxidant and Anti-Melanogenic Activities of Pakoba Leaves (Syzygium sp) from North Celebes

2019 ◽  
Vol 2 (2) ◽  
pp. 79-83
Author(s):  
Dela Rosa ◽  
Catherine Roeroe ◽  
Agustina Susanti
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Chloroform Fraction ◽  
Aqueous Fraction ◽  
Ic50 Value ◽  
Dpph Method ◽  
Good Potential ◽  
Crude Methanol Extract

In this research the potential of Pakoba leaf extract to be used as antioxidant and skin whitening (anti-melanogenic agent) is investigated. The antioxidant activity of Pakoba leaves were studied using DPPH method and the result showed that the 80 percent methanol crude extract has strong antioxidant activity with an IC50 value of 22.66 ± 1.02 μg/ml. The aqueous fraction of the sample has an IC50 value of 53.30 ± 1.42 μg/ml, followed by n-butanol fraction (53.63 ± 1.45 μg/ml) and chloroform fraction (511.54 ± 1.59 μg/ml). The anti-melanogenic activity of the crude methanol extract showed IC50 value of 316.56 ± 11.04 μg/ml. Thus, it isconcluded that crude extract of Pakoba leaves shows good potential as the antioxidant source although it does not show good anti- melanogenic activity.

scholarly journals AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI EKSTRAK DAUN Jatropha gossypifolia L

Alotrop ◽  
2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Nia Sapitri Pangestu ◽  
Nurhamidah Nurhamidah ◽  
Elvinawati Elvinawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Propionibacterium Acnes ◽  
Inhibition Zone ◽  
Ethyl Acetate Fraction ◽  
Activity Test ◽  
Dpph Method ◽  
Jatropha Gossypifolia ◽  
Ethanol Fraction

This study was conducted to determine the activity of Jatropha gossypifolia L (jarak merah’s) leaf extract as an antioxidant and antibactery against acne causing bacteria (Propionibacterium acnes). To obtain an J. gossypifolia L jarak merah’s leaf extract by maceration using 96% ethanol, and then fractionated with n-hexane and ethyl acetate. Testing of antioxidant activity by DPPH method and antibactery activity test by the paper disc method. Antioxidant activity test has done for ethanol fraction, ethyl acetate fraction and n-hexane fraction, while testing for antibacterial activity performed on fractions active as antioxidant. The test results demonstrate the antioxidant activity of ethyl acetate fraction and the ethanol fraction as a fraction very strong with IC50 value of 22.33 ppm for ethyl acetate fraction and 41.04 ppm for ethanol fraction. Then the fraction of ethyl acetate and ethanol fraction antibactery activity test, the result is known that both factions have the ability to inhibit the growth of bacteria Propionibacterium acnes demonstrated by the inhibition zone is formed. In 1000 ppm is formed diameter of the greatest inhibition zone that is equal to 6 mm for ethyl acetate fraction and 5 mm for ethanol fraction.

scholarly journals The Effect of Simplisia Drying Method on Antioxidant Activity of Senggani Fruit Extract (Melastoma Malabathricum L.) by DPPH (2,2-diphenyl-1-picrylhydrazyl)

2021 ◽  
Vol 19 (2) ◽  
pp. 144-153
Author(s):  
Shesanthi Citrariana ◽  
Risqika Yulia Tantri Paramawidhita ◽  
Melliani Melliani
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Degradation Process ◽  
Oxidation Reactions ◽  
Drying Method ◽  
Fruit Extract ◽  
Melastoma Malabathricum ◽  
Ic50 Value ◽  
Study Results ◽  
Dpph Method

Senggani fruit (Melastoma Malabathricum L.) contains anthocyanin that functioning as an antioxidant. Anthocyanin are tremendously sensitive to thermal processes which trigger phytochemical or photo-oxidation reactions that can open anthocyanin rings. The objective of this study is to identify the effect of the simplicia drying method on the antioxidant activity of Senggani fruit extract. Senggani fruit extract was prepared by obtaining samples of ripe fruit, dry sorting, washing, wet sorting, and drying using two methods; sunlight and oven at 70oC. After the simplicia was dry, it was blended and sifted until smooth. The fine simplicia was macerated with 96% ethanol and evaporated to gain a crude extract. The crude extract was assessed with reagents for phytochemical screening. Furthermore, the crude extract was examined for antioxidant activity by the DPPH method. This study implies that the simplicia and crude extract of Senggani fruit from drying in sunlight and oven possess different organoleptic properties such as color, smell, and taste. In phytochemical testing with reagents, it was discovered that anthocyanin compounds were unveiled in drying utilizing sunlight while employing an oven at 70oC; no anthocyanins were found. The antioxidant testing of Senggani fruit extract revealed that the drying method employing sunlight had an IC50 value of 18.8 g/mL while the oven temperature of 70oC owned an IC50 value of 28.3 g/mL. Based on the study results, it can be identified that the simplicia drying method affects the antioxidant activity of the Senggani fruit extract. The drying method in the sun produces extracts with greater antioxidant activity while drying in an oven at 70oC results in a degradation process of anthocyanin compounds, thereby decreasing the antioxidant activity of the Senggani fruit extract.  

scholarly journals Karakteristik Kemasan Aktif dari Film Gelatin Ikan dengan Penambahan Ekstrak Daun Jeruju (Acanthus ilicifolius)

2020 ◽  
Vol 15 (1) ◽  
pp. 63
Author(s):  
Rahmi Nurdiani ◽  
Abdul Aziz Jaziri ◽  
Fitri Septa Puspita
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Active Packaging ◽  
Basic Material ◽  
Leaf Extracts ◽  
Bioactive Substances ◽  
Acanthus Ilicifolius

Kemasan aktif memiliki fungsi yang lebih luas dalam menjaga kualitas suatu produk. Salah satu bahan baku kemasan aktif adalah gelatin dengan penambahan senyawa aktif. Penelitian ini dimaksudkan untuk mempelajari karakteristik dan bioaktivitas kemasan aktif dari gelatin ikan ayam-ayam (Abalistes stellaris) dengan penambahan ekstrak daun jeruju (Acanthus ilicifolius) sebesar 0%, 1%, 3%, 5% dan 7%. Kemasan aktif yang dihasilkan diamati karakteristik dan bioaktifitasnya yang meliputi ketebalan, kuat tarik, elongasi, transmisi uap air, kadar air, pH, aktivitas antioksidan dan aktivitas antibakteri. Hasil analisa sidik ragam menunjukkan penambahan ekstrak daun jeruju berpengaruh nyata (p<0,05) terhadap ketebalan, aktivitas antioksidan dan aktivitas antibakteri dari kemasan aktif berbahan gelatin yang dihasilkan, namun tidak berpengaruh nyata (p>0,05) terhadap kuat tarik, elongasi dan transmisi uap air. Kemasan aktif dari gelatin ikan ayam-ayam dengan penambahan ekstrak daun jeruju 7% memiliki ketebalan 143,01 µm, mampu menghambat 65,45% radikal bebas DPPH dan menghasilkan zona hambat bakteri berkisar antara 2,18-2,61 mm. Hasil penelitian ini menunjukkan gelatin yang terbuat dari kulit dan sisik ikan ayam-ayam dengan penambahan ekstrak daun jeruju memiliki potensi untuk dikembangkan menjadi kemasan aktif.  AbstractPackaging has an important function in maintaining the quality of product. Active packaging can be made from gelatin as basic material with addition of bioactive substances. The purpose of this research was to study the characteristics and bioactivity of active packaging made from staryy triggerfish (Abalistes stellaris) gelatin incorporated with 0, 1, 3, 5, and 7% of Acanthus ilicifolius leaf extract. The active pakaging was observed for its charateristics and bioactivity including thickness,  elongation, tensile strength, water vapor transmission, moisture content, pH, antioxidant activity, and antibacterial activity of active packaging. The statistical analyses showed that the addition of A. ilicifolius leaf extracts significantly affected (p<0.05) the thickness, antioxidant activity, and antibacterial activity of active packaging. Active packaging from fish gelatin containing 7% of A. ilicifolius extract with 143.01 µm thickness, was able to inhibit 65.45% DPPH free radical and resulted in 2.18-2.61 mm inhibition zone. It was suggested that starry triggerfish gelatin and A. ilicifolius leaf extract have a potential to be developed as active packaging.

scholarly journals Antioxidant Activity of Beruas Laut (Scaevola taccada)

Marinade ◽  
2019 ◽  
Vol 2 (01) ◽  
pp. 29-38
Author(s):  
Rudianto ◽  
R. Marwita Sari Putri ◽  
Azwin Apriandi
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Leaf Extract ◽  
Ash Content ◽  
Phytochemical Analysis ◽  
Fruit Extract ◽  
Research Material ◽  
Ic50 Values

This study utilized beruas laut plant (Scaevola taccada) as a research material. The purpose of this study was to determine the antioxidant activity of S. taccada crude extract, especially on leaves and fruit extract. The highest levels of protein was found in the Scaevola taccada leaf (8.806%), the highest fat was found in the leaf (1.822%), the highest water was found in leaf (11.376%), the highest ash content was found in the leaf (14,78%) and the highest carbohydrates content was found in fruit (82.52%). Phytochemical analysis detected flavonoids, tannins, and steroid in the (S. taccada). Antioxidant analysis obtained IC50 values of chloroform leaf extract was 0.1944, methanol leaf extract was 0.1034, chloroform fruit extract was 0.464, and IC50 values of methanol fruit extract was 0.653.

Antibacterial and antioxidant effects of Zanthoxylum armatum DC extracts

2021 ◽  
Vol 50 (1) ◽  
pp. 159-164
Author(s):  
Aqsa Irshad ◽  
Irfan Ullah ◽  
Muhammad Fiaz Khan
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Inhibition Zone ◽  
Fruit Extract ◽  
Zanthoxylum Armatum ◽  
Good Antibacterial Activity ◽  
Maximum Inhibition ◽  
Hexane Extracts ◽  
Antioxidant Effects

Antibacterial and antioxidant activities of three different extracts (acetone, methanol, and n-hexane) of Zanthoxylum armatum fruits and leaves were investigated. The maximum inhibition zone was shown by methanolic leaf extract against K. pneumonia (20.51 ± 0.93). The minimum inhibition zone (12.01 ± 0.93) was exhibited by methanolic fruit extract against S. typhi. The highest antioxidant activity was recorded at 1000 ppm. Methanolic leaf extract was the most antioxidant (89.8%) among all the extracts, followed by methanolic fruit extract (88.6%). The methanolic and acetonic extracts showed good antibacterial activity as compared to n-hexane extracts and n-hexane was less antioxidant.

scholarly journals Bioactive properties of Persea lingue Ness (Lauraceae) fruit and leaf extracts

2023 ◽  
Vol 83 ◽  
Author(s):  
A. Lillo ◽  
F. Carvajal-Caiconte ◽  
W. Vital ◽  
P. I. Silva Junior ◽  
E. J. Paredes-Gamero ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Leaf Extract ◽  
Leukemia Cell ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Fruit Extract ◽  
South Central

Abstract Persea lingue Ness is a tree species that lives mainly in temperate forests of south-central Chile. Its leaves are used in ethnomedicine, the fruit is a drupe similar to that of the avocado and has not been studied. The aim of this study was to determine the cytotoxicity in leukemia cell and antibacterial activity, along with some chemical content characteristics of P. lingue fruit and leaf extracts. The antibacterial activity was determined by the inhibition of bacterial growth in liquid medium assay against Gram-positive and Gram-negative bacteria. The leukemia cell lines Kasumi-1 and Jurkat were used to evaluate the cytotoxic activity by using propidium iodide and AlamarBlue assays. Total phenolic, flavonoid, condensed tannin, alkaloid and lipid contents were evaluated in the fruit and in the leaf extracts. The antioxidant activity of both extracts were also elavaluated. Leaf extract presented the highest content of total phenols, condensed tannins and flavonoids, and also the highest antioxidant activity. While the fruit extract has a higher amount of lipids and alkaloids and the high antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus megaterium and Micrococcus luteus. The leaf extract only showed activity against M. luteus. Concerning the cytotoxic activity, only the fruit extract showed cytotoxicity against the cell lines Jurkat and Kasumi-1. P. lingue fruit extract is a potential source of biologically active molecules for the development of new drugs to be used in some types of leukemia, as well as antibacterial agent.

scholarly journals In vitro activities of Acacia nilotica (L.) Delile bark fractions against Oral Bacteria, Glucosyltransferase and as antioxidant

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ali Mahmoud Muddathir ◽  
Ebtihal Abdalla M. Mohieldin ◽  
Tohru Mitsunaga
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Gallic Acid ◽  
Crude Extract ◽  
Inhibitory Activity ◽  
Oral Bacteria ◽  
Acacia Nilotica ◽  
Assay Method ◽  
Medicinal Uses ◽  
Wide Range

Abstract Background Dental caries and periodontal disease are the most common chronic infectious oral diseases in the world. Acacia nilotica was commonly known in Sudan as Garad or Sunt has a wide range of medicinal uses. In the present study, antibacterial activity of oral bacteria (Streptococcus sobrinus and Porphyromonas gingivalis), inhibitory activity against glucosyltransferase (GTF) enzyme and antioxidant activity were assayed for methanolic crude extract of A. nilotica bark and its fractions. Methods Methanoilc crude extract of A. nilotica bark was applied to a Sephadex LH-20 column and eluted with methanol, aqueous methanol, and finally aqueous acetone to obtain four fractions (Fr1- Fr4). Furthermore, the crude extract and fractions were subjected to analytical high performance liquid chromatography (HPLC). The crude extract and its fractions were assayed for antibacterial activity against S. sobrinus and P. gingivalis using a microplate dilution assay method to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), as well as GTF inhibition and antioxidant activity using ABTS radical scavenging method. Results Fractions (Fr1 and Fr2) exhibited MIC values of 0.3 mg/ml against the P. gingivalis. Additionally, Fr2 displayed MBC value of 1 mg/ml against two types of bacteria. Fr4 showed an especially potent GTF inhibitory activity with IC50 value of 3.9 μg/ml. Fr1 displayed the best antioxidant activity with IC50 value of 1.8 μg/ml. The main compound in Fr1 was identified as gallic acid, and Fr2 was mostly a mixture of gallic acid and methyl gallate. Conclusions The results obtained in this study provide some scientific rationale and justify the use of this plant for the treatment of dental diseases in traditional medicine. A. nilotica bark, besides their antibacterial potentiality and GTF inhibitory activity, it may be used as adjuvant antioxidants in mouthwashes. Further studies in the future are required to identify the rest of the active compounds.

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