Results of urine culture in adults carried out by the microbiology laboratory of the Clinics Hospital - San Lorenzo from january 2015 to august 2016 and methods of study of urinary infections available in the institution

Noting in my small general pediatric practide that the same families kept returning for urine checks following infections as well as for allergy shots, I decided to review all of the current charts to determine the incidence of both problems. "Allergy" includes hay fever, asthma, allergic cough, eczema, bee and other stings, and reaction to foods or drugs severe enough to warrant avoidance, medication, or allergic workup. "G-U infection" includes typical symptoms of bladder irritation with or without pyuria and bacteriuria, acute pyelonephritis diagnosed by the usual symptomatology and laboratory work, and subacute or chronic infections with the children presenting often with more subtle symptoms (tired, cranky, low-grade fever, and off-and-on abdominal pain being the most common complaints) and with a "clean-catch" urine culture of at least 20,000 colonies of one organism.

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659. Evaluation of Four Chromogenic Agars for Urine Culture Including Time and Cost Savings Analysis

Open Forum Infectious Diseases ◽

10.1093/ofid/ofaa439.852 ◽

2020 ◽

Vol 7 (Supplement_1) ◽

pp. S386-S386

Author(s):

Eve Capistran ◽

Simon Lévesque ◽

Philippe Martin ◽

Diane Girard ◽

Marie-Eve Papirakis ◽

...

Keyword(s):

Primary Outcome ◽

Susceptibility Testing ◽

Urine Culture ◽

Tertiary Care ◽

Cost Savings ◽

Turnaround Time ◽

Microbiology Laboratory ◽

University Center ◽

And Performance ◽

Time Required

Abstract Background With a volume of approximately 5000 urine culture specimens per month in our tertiary-care university center hospital’s microbiology laboratory, we wanted to evaluate methods aiming to improve workflow and performance while reducing turnaround time and potentially overall cost. Methods 310 urine culture specimens as well as selected less frequent pathogens (A. urinae - 26 strains, C. urealyticum - 4 strains) were plated on four chromogenic agars in parallel with standard protocol MacConkey (MAC) and blood agar (BA). Chromogenic agars evaluated were: UriSelectTM 4 (Bio-Rad), CHROMID® CPS® Elite (bioMérieux), BrillanceTM UTI ClarityTM agar Biplate (Oxoid) and BDTM CHROMagarTM Orientation (BD). Primary outcome was overall growth performance for frequent pathogens and for gram positives, where chromogenic agars were previously reported to underperform.The number of additional tests needed and the appreciation of different media by laboratory personnel were also assessed. A sub-analysis measured the total time required to plate and to read 50 consecutive specimens comparatively for the 4 chromogenic agars and for MAC/BA. Results Global performance was 90% for Uriselect, 88% for ChromID, 89% for Chromagar and 81% for Brillance compared to 84% for standard method. ChromID and Brillance supported the growth of more A. urinae and C. urealyticum than the other 2 chromogenic agars. All monoplate chromogenic agars were appreciated equally by technologists. In addition, for all chromogenic agars, working time was reduced by half as compared to MAC/BA. We estimated a time economy of approximately 80 hours per month in our laboratory, translating in a net annual economy. Conclusion All 4 chromogenic medias evaluated in our study had an acceptable performance, with specific strengths and weaknesses for each one. The choice of ChromID CPS Elite (bioMérieux) for our center was based on pre-established criteria including performance for more fastidious gram positives, best time and cost economy, and compatibility with current identification method and susceptibility testing platform. However, since the 4 chromogenic agars have been adequately verified in our laboratory, we consider that they could be interchangeable if needed. Disclosures All Authors: No reported disclosures

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Analysis of urine culture isolates from microbiology laboratory of a tertiary care Hospital

Indian Journal of Microbiology Research ◽

10.18231/j.ijmr.2019.022 ◽

2019 ◽

Vol 6 (2) ◽

pp. 106-108

Author(s):

Nisarg A Trivedi ◽

◽

Rahul B Lunagaria ◽

Keyword(s):

Tertiary Care Hospital ◽

Urine Culture ◽

Tertiary Care ◽

Care Hospital ◽

Microbiology Laboratory ◽

Analysis Of Urine

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Impact of Introduction of the BD Kiestra InoqulA on Urine Culture Results in a Hospital Clinical Microbiology Laboratory

Journal of Clinical Microbiology ◽

10.1128/jcm.00417-15 ◽

2015 ◽

Vol 53 (5) ◽

pp. 1736-1740 ◽

Cited By ~ 13

Author(s):

Sharon Strauss ◽

Paul P. Bourbeau

Keyword(s):

Clinical Microbiology ◽

Urine Culture ◽

Clinical Microbiology Laboratory ◽

Microbiology Laboratory

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2340. Diagnostic Stewardship: Survey of Urine Culturing and C. difficile Testing Practices Amongst Oregon Microbiology Labs

Open Forum Infectious Diseases ◽

10.1093/ofid/ofz360.2018 ◽

2019 ◽

Vol 6 (Supplement_2) ◽

pp. S804-S805

Author(s):

Angela H Villamagna ◽

P Maureen Cassidy ◽

Rebecca Pierce ◽

Dat Tran ◽

Chad Nix ◽

...

Keyword(s):

Response Rate ◽

Urine Culture ◽

Accurate Diagnosis ◽

Microbiology Laboratory ◽

Healthcare Facilities ◽

Bacterial Cultures ◽

Testing Algorithms ◽

Testing Practices ◽

Definition Of ◽

Unnecessary Testing

Abstract Background Testing for urinary tract infection (UTI) and Clostridiodes difficile infection (CDI) poses diagnostic and antimicrobial stewardship challenges. Both diagnoses hinge on local microbiology laboratory algorithms. For UTI testing, the definition of “abnormal” urinalysis and the use of reflex urine cultures, both of which alter the frequency of bacteriuria detection, likely differs between laboratories. For CDI, pretest probability, choice and sequence of diagnostic tests are likely variable and impact the chances of accurate diagnosis. Methods To understand laboratory practices and determine variations in local testing algorithms, we deployed a self-administered survey to microbiology laboratories serving Oregon healthcare facilities via SurveyMonkey in September 2018. Responses were collected through April 2019. We analyzed a subset of questions focused on UTI and CDI diagnosis. Results Of 51 surveyed laboratories, response rate was 86% (n = 44). 91% of respondents (n = 40) process bacterial cultures. 47.5% (n = 19) primarily perform urine culture when ordered, whereas the remainder primarily perform cultures in a reflex algorithm when ordered (n = 12; 30%) or a reflex algorithm automatically (n = 9; 22.5%) (Figure 1). The definition of an abnormal urinalysis varied widely (Figure 2). 15% (n = 6) of laboratories reported considering changes to their workflow; two cited a goal of reducing unnecessary testing. Of the 32 laboratories that perform in-house C. difficile testing, the assays and sequence in which they were implemented in testing algorithms varied substantially (Figure 3) and most commonly included NAAT testing. Seven (21.8%) laboratories reported recently changed practices; these changes did not favor any particular algorithm. 84.2% (n = 32) reported stool rejection criteria to limit unnecessary testing, but these criteria varied (Figure 4). Conclusion Wide variation exists in laboratory workflows for UTI and CDI diagnoses in Oregon, suggesting lack of consensus on optimal practices. Encouragingly, multiple labs described recently implemented or planned interventions to reduce unnecessary testing for both infections. This snapshot will inform statewide education and interventions to optimize testing and help prevent patient and population harm. Disclosures All authors: No reported disclosures.

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Infeções Urinárias Causadas por Enterobacteriaceae Produtoras de β-Lactamases de Espetro Expandido Adquiridas na Comunidade num Hospital de Nível III - Um Estudo Retrospetivo

Acta Médica Portuguesa ◽

10.20344/amp.12338 ◽

2020 ◽

Vol 33 (7-8) ◽

pp. 466

Author(s):

Ana Simões ◽

Margarida Lima ◽

Ana Brett ◽

Carolina Queiroz ◽

Catarina Chaves ◽

...

Keyword(s):

Escherichia Coli ◽

Retrospective Study ◽

Urinary Tract ◽

Urinary Tract Infections ◽

Urine Culture ◽

Urinary Infection ◽

Proteus Vulgaris ◽

Paediatric Hospital ◽

Urinary Infections ◽

Tract Infections

Introduction: The emergence of β-lactamases producing bacteria is a problem worldwide, with increasing importance in communityacquired infections, especially in urinary tract infections. Data regarding the use of non-carbapenem antimicrobials in these infections are scarce. The aim of this study was to analyse the treatment and outcome of urinary tract infections caused by community-acquired β-lactamase-producing bacteria in children.Material and Methods: Retrospective study performed in a level III paediatric hospital, between June 2007 and December 2017. All children with β-lactamase-producing Enterobacteriaceae identified in aseptically collected urine culture were included.Results: A total of 175 urinary infections caused by β-lactamases producing bacteria were diagnosed, 34 (19%) were community-acquired: 25 Escherichia coli (74%), 4 Klebsiella pneumoniae (12%), 4 Proteus mirabilis (12%) and 1 Proteus vulgaris (3%). In 30 (88%) cases, it was the first urinary infection. After identification of the microorganism and antimicrobial susceptibility, 33 (97%) children were re-evaluated and 24 (71%) had a repeat urine culture, which was positive in three (13%). In six (18%) cases, antibiotic treatment was modified. Four (12%) children had another UTI in the following month. In 30 (88%) children, imaging was carried out, with no nephrourological malformations detected.Discussion: In the last decade, about 20% of urinary infections caused by β-lactamase-producing Enterobacteriaceae were community-acquired with a relatively stable number of cases over the years. No nephro-urological malformations were identified in these children.Conclusion: Although the number of cases is small, the clinical and microbiological outcomes showed that most were successfully treated with non-carbapenem antibiotics, with low recurrence of new episodes of urinary tract infections.

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Utility of the blood culture in infection of the urinary tract with fever in the elderly

Revista Española de Quimioterapia ◽

10.37201/req/156.2020 ◽

2021 ◽

Author(s):

Elisa Álvarez Artero ◽

Amaia Campo Núñez ◽

Moisés Garcia Bravo ◽

Inmaculada García García ◽

Moncef Belhassen Garcia ◽

...

Keyword(s):

Risk Factors ◽

Urinary Tract ◽

Urine Culture ◽

Urinary Infection ◽

The Elderly ◽

Blood Cultures ◽

Febrile Urinary Tract Infection ◽

Urinary Infections ◽

Tract Infections ◽

Overall Mortality

Introduction. Urinary tract infections are one of the most common community infections. The diagnosis of urinary infections in the elderly is complex because of its presentation and clinic. The aim of this article is to evaluate the usefulness of blood cultures in febrile urinary tract infection in elderly patients, risk factors, causes of discordance between urine and blood cultures, usefulness of biomarkers and mortality. Material and methods. Observational study of patients admitted over 65 years old, with urinary infections. Results. A total of 216 episodes with urinary infections and blood cultures performed. 70 (32,4%) cases with bacteremia. The most frequently detected isolates in blood cultures were: Escherichia coli 50 (71,4%) and Proteus spp. 6 (8,5%). Only septic shock was associated with a higher frequency of bacteraemia (OR=2,93, IC 95: 1,0-8,5; p=0,04). In 26 of the blood cultures a different isolation of the urine culture was detected. Overall mortality was 9.1%, with no association with the presence of bacteremia (p>0. 05). Conclusions. One third of elderly people hospitalized by tract urinary infection had bacteremia. Their detection was not associated with overall mortality. Disagree between blood and urine cultures in febrile is frequent, especially in patients with recent antibiotic treatment or recently hospitalized.

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Agreement between Parallel Canine Blood and Urine Cultures: Is Urine Culture the Poor Man's Blood Culture?

Journal of Clinical Microbiology ◽

10.1128/jcm.00506-18 ◽

2018 ◽

Vol 56 (9) ◽

Author(s):

Nanelle R. Barash ◽

Adam J. Birkenheuer ◽

Shelly L. Vaden ◽

Megan E. Jacob

Keyword(s):

Blood Culture ◽

Urine Culture ◽

Bloodstream Infections ◽

Kappa Statistic ◽

Blood Cultures ◽

Predictive Values ◽

Content Type ◽

Urogenital Infection ◽

Urinary Infections ◽

Urine Specimens

ABSTRACT Bloodstream infections are a significant cause of morbidity and mortality in critically ill dogs, but due to cost and difficulties in sample acquisition, blood cultures are infrequently obtained. In ill dogs, urine cultures may be recommended as surrogates for blood cultures. In order to determine the outcome agreement between parallel urine and blood cultures, we retrospectively analyzed parallel blood and urine specimens submitted for culture from dogs at the NC State Veterinary Hospital between 2011 and 2016. Positive cultures were reported from 15% of the submitted blood specimens and 23% of the submitted urine specimens. A total of 295 urine and blood samples were submitted in parallel, with positive growth demonstrated in 14 concordant and five discordant pairs. A kappa statistic comparing blood and urine culture outcomes was 0.266 (fair) when all parallel growth was included, including concordant and discordant results, and 0.170 (poor) when restricted to parallel concordant growth. The sensitivity of urine to reflect concordant bloodstream bacterial organisms was 30%, with a specificity of 87%. The positive and negative predictive values were 30% and 88%, respectively. Of dogs with both specimens positive on bacterial culture, 7 of 7 (100%) with suspected urogenital infection sources were concordant. All dogs with discordant bloodstream and urinary infections were immunosuppressed. Urinary coagulase-positive Staphylococcus isolates were most likely to be concordant with bloodstream infections. In conclusion, we found that urine culture is neither a substitute nor a screen for blood culture. Blood cultures should be performed in any potentially septic animal, especially those that are considered immunosuppressed.

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Efektifitas Ekstrak Daun Sirih Hijau (Piper Betle) sebagai Insektisida Nabati terhadap Mortalitas Lalat Rumah (Musca domestica)

Jurnal Kesehatan Terpadu (Integrated Health Journal) ◽

10.32695/jkt.v10i2.42 ◽

2019 ◽

Vol 10 (2) ◽

pp. 44-50

Author(s):

Rinaldi Daswito ◽

Rima Folentia ◽

M Yusuf MF

Keyword(s):

Musca Domestica ◽

Leaf Extract ◽

Effective Concentration ◽

P Value ◽

Piper Betle ◽

Microbiology Laboratory ◽

House Flies ◽

Betel Leaf ◽

The One ◽

One Way Anova

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly. Keywords: Green betel leaf extract , organic insecticide, houseflies

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