scholarly journals AUTOTOXIC EFFECT OF Anagyris foetida L. ALKALOIDS CONTENT

2021 ◽  
Vol 9 (3) ◽  
pp. 362-368
Author(s):  
Samar Emad Ezzat ◽  
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Integrated Management ◽  
Root Extract ◽  
Aqueous Extracts ◽  
Poisonous Plants ◽  
Minimum Concentration ◽  
Germination And Growth ◽  
Allelopathic Compounds ◽  
Plant Germination

The present study aimed to evaluate the autotoxic effect of Anagyrus foetida root, flower, and leaves alkaloids on the seed germination and growth of the same species. The pot experiment was conducted for three concentrations i.e. 4, 8, and 12% of aqueous extracts of root, flower, and leaves. Further, the seed and leaves were evaluated for their alkaloids content i.e. cytisine, aporphine, and anagyrine by high-performance liquid chromatography. The results of the study suggested that seed treated by 4% root extract showed a decrease in aporphine, cytisine, and anagyrine. While the seed treated with the flower and leaves extract (12%) showed a lower concentration of all three alkaloids as compared to untreated control. While a mixture of all three parts extracts at 4% concentration showed a minimum concentration of aporphine, cytisine, and anagyrine alkaloids in the treated seeds. Anagyrus foetida roots effectively secret allelopathic compounds which inhibit A. foetida plant germination and growth. The allelopathy effect of plants can be studied in the integrated management of poisonous plants. Results of the study suggested that the farmers should not repeat the A. foetida plantation continue two successive years.

scholarly journals Scopoletin: Antiamyloidogenic, Anticholinesterase, and Neuroprotective Potential of a Natural Compound Present in Argyreia speciosa Roots by In Vitro and In Silico Study

2020 ◽  
Vol 15 ◽  
pp. 263310552093769 ◽  
Author(s):  
Priya Kashyap ◽  
Heera Ram ◽  
Sunil Dutt Shukla ◽  
Suresh Kumar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Enzyme Inhibition ◽  
In Silico ◽  
High Performance ◽  
Amyloid Β ◽  
Root Extract ◽  
Aβ Peptides ◽  
Disease Modifying

Alzheimer’s disease (AD) is characterized by depositions of amyloid β (Aβ) peptides aggregates resulting in plaques formation in the central nervous system (CNS). This study evaluates the disease-modifying potential of scopoletin against multiple factors associated with AD such as cholinesterase enzymes, Aβ peptides, and neuroprotective properties against Aβ- and H2O2-induced cytotoxicity under in vitro conditions. Scopoletin was identified and quantified using UPLC-QTOF (ultra-high performance liquid chromatography-quadrupole time-of-flight) and high-performance liquid chromatography (HPLC), respectively. The antiamyloidogenic potential was evaluated by thioflavin T and congo red binding assay. Inhibition of key enzymes, that is, acetylcholinesterase and butyrylcholinesterase, was investigated by Ellman’s assay. UPLC-QTOF analysis showed that most abundant phytoconstituent present in Argyreia speciosa hydroalcoholic root extract was scopoletin followed by festuclavine and ergometrine. Scopoletin was further quantified using novel reverse phase (RP)-HPLC method developed in this study. The neuroprotective potential of scopoletin was found to be 69% against Aβ42-induced neurotoxicity and 73% against H2O2-induced cytotoxicity in PC12 cell culture at 40 μM final concentration. At the same concentration, scopoletin inhibited Aβ42 fibril formation up to 57%. The IC50 concentration for AChE and BuChE enzyme inhibition by scopoletin was 5.34 and 9.11 μM, respectively. The antiaggregation and enzyme inhibition results were complemented with strong molecular interactions of scopoletin with target proteins validated by in silico molecular docking analysis. Based on this study, it can be concluded that scopoletin can be used as a lead for amelioration of symptoms and disease-modifying effects in AD.

scholarly journals DETERMINATION OF VULCANIZATION ACCELERATORS IN ANALYSIS OF POLYMER MATERIALS BY HPLC

2018 ◽  
Vol 54 (3) ◽  
pp. 289-295
Author(s):  
A. V. Yukhnik ◽  
S. M. Leschev
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Analytical Signal ◽  
Calibration Graph ◽  
Polymer Materials ◽  
Internal Diameter ◽  
Aqueous Extracts ◽  
Gradient Separation ◽  
Test Objects

A method of simultaneous determination of altax, captax, thiuram D, thiuram E, thimate and ethylthimate in aqueous extracts in the sanitary-chemical analysis by high performance liquid chromatography has been developed. Determination was based on the gradient separation of altax, captax, thiuram D, thiuram E, thimate, ethylthimate extracted by water from test objects using Waters XTerra MS C18 column of 250 mm length, internal diameter 4.6 mm, graining phase 5 μm, while UV detection wavelengths were 265 nm and 320 nm. Retention times were 10.3±0.2 min for altax, 3.6±0.2 min for captax, 9.0±0.2 min for thiuram D, 12.3±0.2 min for thiuram E, 11.0±0.2 min for thimate, 15.5±0.2 min for ethylthimate. It has been shown that the method is linear in the range of 0.05–0.60 mkg/ml for altax, 0.005–0.60 mkg/ml for captax, 0.005–0.75 mkg/ml for thiuram D and thiuram Е, 0.01–0.90 mkg/ml for thimate and ethylthimate. Using the calibration graph and standard deviations of analytical signal, following limits of quantification were calculated: 0.01 mkg/ml for altax, 0.002 mkg/ml for captax, 0.003 mkg/ml for thiuram D, 0.005 mkg/ml for thiuram Е, 0.01 mkg/ml for thimate and ethylthimate.

scholarly journals Growth of white clover seedlings treated with aqueous extracts of leaf and root of tough lovegrass

2011 ◽  
Vol 40 (6) ◽  
pp. 1168-1172 ◽  
Author(s):  
Adriana Favaretto ◽  
Simone Meredith Scheffer-Basso ◽  
Vanderleia Felini ◽  
Alana Neto Zoch ◽  
Cercí Maria Carneiro
Keyword(s):  
White Clover ◽  
Leaf Extract ◽  
Chemical Nature ◽  
Morphological Changes ◽  
Root Extract ◽  
Aqueous Extracts ◽  
Leaf Aqueous Extract ◽  
Normal Seedling ◽  
Allelopathic Compounds ◽  
Secondary Roots

This study was carried out to investigate the effect of extracts of leaf and roots of tough lovegrass (Eragrostis plana) on seedling growth of white clover (Trifolium repens), in two experiments. The first experiment was conducted as a pilot test, in which two concentrations (30 and 60%) of leaf aqueous extract were tested. In Experiment II, source (leaf/root) and concentration (5 and 10%) of the extracts were combined. Distilled water was used as control in both trials. Fifty seedlings at 7 days of age were placed in gerbox containers on filter paper moistened with extracts and incubated at 25°C for 14 days. In the first experiment, percentage of normal seedlings decreased by 82.5% and 100% at the concentrations of 30% and 60%, respectively, which indicated the need to increase the dilution of the extracts. In the subsequent experiment, the extracts from the two sources, at 10% of concentration, reduced by 78% the percentage of normal seedling and increased the percentage of abnormal seedlings, which reached 27%. At 5% concentration, the leaf extract was the most deleterious treatment, resulting in greater mortality (M=27.6%) and abnormality (A=19.5%) of seedlings, compared to the root extract (M=4.8%; A=9.5%) and the control (M=2.4%; A=0.25%). The morphological changes caused by the extracts were radicle necrosis, retention of cotyledons and absence of secondary roots. The results suggest that allelopathic compounds from leaves and roots of tough lovegrass differ in chemical nature and/or concentration.

scholarly journals Assessment of the Use of Natural Extracted Dyes and Pancreatin Enzyme for Dyeing of Four Natural Textiles: HPLC Analysis of Phytochemicals

Processes ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 59 ◽  
Author(s):  
Mohamed Z. M. Salem ◽  
Ibrahim H. M. Ibrahim ◽  
Hayssam M. Ali ◽  
Hany M. Helmy
Keyword(s):  
High Performance Liquid Chromatography ◽  
Salicylic Acid ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Color Difference ◽  
Root Extract ◽  
Color Strength ◽  
Dyeing Process ◽  
Total Color Difference

In the present study, four natural textiles (cotton, linen, wool, and silk) were dyed with 14 naturally extracted dyes, and pancreatin enzyme was used in the dyeing process. The effects of pancreatin enzyme and its buffer on naturally dyed textile samples were evaluated. Two concentrations of pancreatin enzyme and buffer were used as pretreatments for dyed textiles. Proteinic fabrics showed the highest relative color strength (RCS) values of 137.23% and 132.2% when the pancreatin enzyme was applied on wool and silk dyed with pomegranate skin and bloodroot at concentrations A and B, respectively. Linen fiber dyed with catechu tree showed the highest total color difference (TCD) values with buffer (6.83) and pancreatin enzyme A (5.7) and B (6.3). This shows that there were no side effects of the pancreatin enzyme on the studied dyed textiles. By high-performance liquid chromatography (HPLC) analysis, the root extract from madder showed the presence of salicylic acid (1758.91 mg/kg extract), quercetin (844.23 mg/kg extract), ellagic acid (784.86 mg/kg extract) and benzoic acid (582.68 mg/kg extract) as main compounds. In cochineal extract the main compounds were rutin (37.732 mg/kg extract), kampherol (1915.98 mg/kg extract), myricetin (809.97 mg/kg extract), quercetin (496.76 mg/kg extract) and salicylic acid (193.87 mg/kg extract).

Sign in / Sign up

Export Citation Format

Share Document