scholarly journals Growth of white clover seedlings treated with aqueous extracts of leaf and root of tough lovegrass

2011 ◽  
Vol 40 (6) ◽  
pp. 1168-1172 ◽  
Author(s):  
Adriana Favaretto ◽  
Simone Meredith Scheffer-Basso ◽  
Vanderleia Felini ◽  
Alana Neto Zoch ◽  
Cercí Maria Carneiro
Keyword(s):  
White Clover ◽  
Leaf Extract ◽  
Chemical Nature ◽  
Morphological Changes ◽  
Root Extract ◽  
Aqueous Extracts ◽  
Leaf Aqueous Extract ◽  
Normal Seedling ◽  
Allelopathic Compounds ◽  
Secondary Roots

This study was carried out to investigate the effect of extracts of leaf and roots of tough lovegrass (Eragrostis plana) on seedling growth of white clover (Trifolium repens), in two experiments. The first experiment was conducted as a pilot test, in which two concentrations (30 and 60%) of leaf aqueous extract were tested. In Experiment II, source (leaf/root) and concentration (5 and 10%) of the extracts were combined. Distilled water was used as control in both trials. Fifty seedlings at 7 days of age were placed in gerbox containers on filter paper moistened with extracts and incubated at 25°C for 14 days. In the first experiment, percentage of normal seedlings decreased by 82.5% and 100% at the concentrations of 30% and 60%, respectively, which indicated the need to increase the dilution of the extracts. In the subsequent experiment, the extracts from the two sources, at 10% of concentration, reduced by 78% the percentage of normal seedling and increased the percentage of abnormal seedlings, which reached 27%. At 5% concentration, the leaf extract was the most deleterious treatment, resulting in greater mortality (M=27.6%) and abnormality (A=19.5%) of seedlings, compared to the root extract (M=4.8%; A=9.5%) and the control (M=2.4%; A=0.25%). The morphological changes caused by the extracts were radicle necrosis, retention of cotyledons and absence of secondary roots. The results suggest that allelopathic compounds from leaves and roots of tough lovegrass differ in chemical nature and/or concentration.

scholarly journals AUTOTOXIC EFFECT OF Anagyris foetida L. ALKALOIDS CONTENT

2021 ◽  
Vol 9 (3) ◽  
pp. 362-368
Author(s):  
Samar Emad Ezzat ◽  
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Integrated Management ◽  
Root Extract ◽  
Aqueous Extracts ◽  
Poisonous Plants ◽  
Minimum Concentration ◽  
Germination And Growth ◽  
Allelopathic Compounds ◽  
Plant Germination

The present study aimed to evaluate the autotoxic effect of Anagyrus foetida root, flower, and leaves alkaloids on the seed germination and growth of the same species. The pot experiment was conducted for three concentrations i.e. 4, 8, and 12% of aqueous extracts of root, flower, and leaves. Further, the seed and leaves were evaluated for their alkaloids content i.e. cytisine, aporphine, and anagyrine by high-performance liquid chromatography. The results of the study suggested that seed treated by 4% root extract showed a decrease in aporphine, cytisine, and anagyrine. While the seed treated with the flower and leaves extract (12%) showed a lower concentration of all three alkaloids as compared to untreated control. While a mixture of all three parts extracts at 4% concentration showed a minimum concentration of aporphine, cytisine, and anagyrine alkaloids in the treated seeds. Anagyrus foetida roots effectively secret allelopathic compounds which inhibit A. foetida plant germination and growth. The allelopathy effect of plants can be studied in the integrated management of poisonous plants. Results of the study suggested that the farmers should not repeat the A. foetida plantation continue two successive years.

scholarly journals Allelopathic effects of aqueous extracts of Artistolochia esperanzae O.Kuntze on development of Sesamum indicum L. seedlings

2010 ◽  
Vol 24 (2) ◽  
pp. 454-461 ◽  
Author(s):  
Ana Beatriz Gatti ◽  
Alfredo Gui Ferreira ◽  
Marcos Arduin ◽  
Sonia Cristina Gualtieri de Andrade Perez
Keyword(s):  
Root Growth ◽  
Growth And Development ◽  
Morphological Changes ◽  
Primary Root ◽  
Sesamum Indicum ◽  
Aqueous Extracts ◽  
Root Extracts ◽  
Climbing Plant ◽  
Allelopathic Effects ◽  
Secondary Roots

Aristolochia esperanzae is a climbing plant that occurs in the savanna regions of Brazil. The aim of this work was to identify the effects of aqueous extracts of A. esperanzae on germination, root growth and xylem cell development of sesame seedlings. Leaf and shoot extracts were prepared at concentrations of 1.5 and 3%. Extracts caused marked changes in germination and seedling growth with greatest inhibition produced by root extracts. Morphological changes and decreased growth and development of seedlings were also observed. The extracts of A. esperanzae caused a reduction of 50% in the size of root xylem cells and marked changes in the primary root and in the number of secondary roots.

scholarly journals Studies on the Phytochemical Compounds in the Ethanolic Leaf Extract (ELE), Ethanolic Bark Extract (EBE) and Ethanolic Root Extract (ERE) of Bridelia ferruginea Benth (Euphorbiaceae)

2019 ◽  
pp. 1-8 ◽  
Author(s):  
Abdullahi Attah Alfa ◽  
Orukotan Abimbola Ayodeji ◽  
Goji Anthony Donatus Teru ◽  
Kokori Bajeh Tijani
Keyword(s):  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Bioactive Compound ◽  
Fats And Oils ◽  
Bark Extract ◽  
Root Extract ◽  
Suction Pump ◽  
Plant Parts ◽  
Bridelia Ferruginea ◽  
Phytochemical Compounds

The phytochemical compounds of Bridelia ferruginea plant parts was carried out using qualitative method to determine the bioactive compounds present in the plant leave, stem bark and root extracts. The samples was weighed, of which 100 g each of the powder were extracted in solvents (ethanol) 1000 ml macerated and stand for 72 hours. The solvents contained in the maceration bottle was decanted and filtered using a filter paper, the filtration was aided using a suction pump. The filtrate was concentrated using a rotary evaporator and then transferred into thermostatic water cabinet (Temperature was set at 45oC), allowed to dry completely. The plant parts extracts were separately kept in a screw capped bottle for further research. The bioactive compound in the plant parts were detected. The result revealed that Carbohydrates, Saponins, Flavonoids, Tannins, Cardiac Glycosides, fats and oils were present. Alkaloid present in Dragendoff’s test in all plant parts extract but absent in Mayer’s test in only leaf extract. Terpenoids/Steroids present in Liebermann-Burchard’s test in all plant parts extract but absent in Salkowski’s test in only leaf extract. Anthraquinones were absent in all plant parts extracts using Bontrager’s test. Therefore, the presence of these phyto-pharmacological compounds is an indicative that the plant is medicinal and it can be used for the treatment of bacterial and other microbial infections. Further study can be done to separate the individual metabolites to test their antimicrobial activity against some pathogenic bacteria like bacterial meningitis, tuberculosis and syphilis to determine their potency.

scholarly journals Acute and sub-acute oral toxicity of aqueous whole leaf and green rind extracts of Aloe vera in Wistar rats

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Florence Nalimu ◽  
Joseph Oloro ◽  
Emanuel L. Peter ◽  
Patrick Engeu Ogwang
Keyword(s):  
Toxicity Test ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Aloe Vera ◽  
Female Rats ◽  
Male Rats ◽  
Aqueous Extracts ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Leaf Extracts

Abstract Background Several local communities in Central, Western, Eastern, and Northern regions of Uganda have been using the whole leaf extracts of Aloe vera (L.) Burm. f. (Asphodelaceae) in the treatment of various ailments. Also, several commercial companies sell A. vera as soft drinks in Uganda. However, there are inadequate reports on the toxicities of such preparations. This paper reports the acute and sub-acute oral toxicity of aqueous extracts of whole leaf and green rind of A. vera in Wistar rats. Methods Acute oral toxicity test was carried out in female Wistar rats at doses of 175, 550, 1750, and 5000 mg/kg, p.o. The animals were observed for signs of toxicity for 14 days. Similarly, a sub-acute oral toxicity test was performed in both sexes of rats at doses of 200, 400, and 800 mg/kg, p.o. daily for 28 days. All the groups of animals were monitored for behavioral, morphological, biochemical, and physiological changes, including mortality and compared with respective controls. Body weights were measured weekly while the animals’ relative organ weights, hematological, biochemical, gross, and microscopic pathology were examined on day 29. Results There was no mortality or apparent behavioral changes at the doses tested in acute and sub-acute oral toxicity tests. Thus, the Median Lethal Dose (LD50) of green rind and whole leaf aqueous extracts was above 5000 mg/kg. Gross anatomy revealed that the rats’ relative spleen weight in green rind extract at 200 mg/kg significantly decreased compared to the control group. The creatinine levels in female rats that received green rind extract and the chloride ion levels in male rats administered whole leaf extract were significantly elevated. Conversely, Mean Corpuscular Hemoglobin Concentration (MCHC) levels significantly decreased at lower doses of the green rind extract compared to the control. Histopathology of the kidney revealed the renal interstitium’s inflammation at doses of 200 and 800 mg/kg of the whole leaf extract. Conclusion The findings demonstrated that A. vera green rind and whole leaf extracts are non-toxic at relatively high doses when used for a short duration. Prolonged use of the aqueous whole leaf extract might be associated with kidney toxicity.

scholarly journals Antifeedant Activity of Leaf Aqueous Extracts of Selected Medicinal Plants on VI Instar Larva of Helicoverpa Armigera (Hubner)

2020 ◽  
Vol 8 (05) ◽  
pp. 62-66
Author(s):  
G. Ravi ◽  
Dr.G. Sundararajan
Keyword(s):  
Mortality Rate ◽  
Medicinal Plants ◽  
Aqueous Extract ◽  
Helicoverpa Armigera ◽  
Botanical Insecticides ◽  
Tribulus Terrestris ◽  
Aqueous Extracts ◽  
Antifeedant Activity ◽  
Leaf Aqueous Extract ◽  
Helicoverpa Armígera

Phytochemicals, especially botanical insecticides are currently of interest because of their successful application in plant protection as biocontrol agents. Biological activity of leaf aqueous extract of ten selected medicinal plants were evaluated against the fourth-instar larvae of gram pod borer Helicoverpa armigera (H¨ubner), (Lepidoptera: Noctuidae). Antifeedant activity of leaf aqueous extracts of Abutilon indicum L., Achyranthus aspera L., Aerva lanata L., Albiziz amara (Roxb), Andrographis paniculata Ness., Cardiospermum halicacabum L., Cassia tora L., Catharanthus roseus L (G) Don., Datura metal L. and Tribulus terrestris L. were evaluated in this study. Preliminary screening after 24 h of exposure with leaf aqueous extracts of the selected plants at a concentration of 1,000 ppm exhibited significant larval mortality rate. The percentage mortality rate ranged considerably from 10.8 to 72.8. The mortality rate was observed in the decreasing order of A. paniculata > C. roseus > D. metal > A. amara > C. halicacabum > A. indicum > C. tora > T. terrestris > A. aspera >A. lanata against the larvae of H. armigera. The results imply that leaf aqueous extract of A. paniculata, C. roseus and D. metal can potentially be used as eco-friendly pest control agents against the larva of H. armigera.

scholarly journals Determination of the inorganic composition and short term effect of aqueous extracts of root, pod, and stem of Telfairia occidentalis on some hematological parameters in rats

2016 ◽  
Vol 7 (5) ◽  
pp. 75-80
Author(s):  
Enyinnaya A Ogbonnaya ◽  
Patrick O Uadia
Keyword(s):  
Blood Cell ◽  
Hematological Parameters ◽  
Atomic Absorption Spectrophotometry ◽  
Mineral Elements ◽  
Root Extract ◽  
Aqueous Extracts ◽  
Telfairia Occidentalis ◽  
Stem Extract ◽  
Inorganic Composition ◽  
Wbc Count

Aims and Objectives: This study is to investigate the inorganic composition and the effect of fourteen (14) -day oral administration of aqueous extracts of root, pod and stem of Telfairia occidentalis in rats.Materials and Methods: Plant samples were analyzed for K, P, Mg, Ca, S, Mn, Fe, Cu, Zn, Pb, and Cr using atomic absorption spectrophotometry (AAS). Wister rats of both sexes were assigned to sixteen (16) groups of 4 animals per group. Different animal groups received distilled water and   root, stem, and pod extracts at the doses of 250, 750, 1500, 2250, and 3000mg/kg of body weight. All animals were treated for 14 days and sacrificed on the 15th day.Results: The inorganic composition result showed that potassium (K) was the highest in abundance in all the samples (root, pod, and stem), compared to the other mineral elements. The root contained high levels of potassium and manganese; the stem had magnesium, manganese and iron while the pod contained high amount of potassium, manganese and iron. Hematological assay results showed reduction in the values of white blood cell (WBC) count for higher doses of root extract and all doses (except for 1500mg/kg bw) of pod extract. Platelet count (PLT) was significantly high for root extract at 750mg/kg bw. The stem extract showed a consistent decrease in the red blood cell (RBC) count for all dose administered.Conclusion: Telfairia occidentalis root, pod, and stem may be a good source of inorganic elements. The extracts have varying effects on hematological parameters which may be dosage- and duration- dependent.Asian Journal of Medical Sciences Vol.7(5) 2016 75-80

scholarly journals Hepatoprotective Effect of Costus Afer On Trace Metal Mixture Treated Rats Mediated By Regulation of Oxidative Stress Markers, Inflammatory Cytokines And Bio-Metal Chelation

2021 ◽  
Author(s):  
Brilliance Onyinyechi Anyanwu ◽  
Anthonet N. Ezejiofor ◽  
Ify L. Nwaogazie ◽  
Orish Ebere Orisakwe
Keyword(s):  
Trace Metal ◽  
Inflammatory Cytokines ◽  
Leaf Extract ◽  
Morphological Changes ◽  
Lead Chloride ◽  
Laboratory Animals ◽  
Mercury Chloride ◽  
Metal Chelation ◽  
Metal Mixture ◽  
Costus Afer

Abstract Technological developments have led to exposure to various substances that are harmful to the environment and public health, including heavy metals. In the environment, these grades of metals are usually diverse mixtures shown to cause physiological, biochemical and neurological dysfunctions in humans and laboratory animals. Cadmium, Lead, and mercury have been envisaged to exhibit their hepatotoxic effects by oxidative induction damage and synthesis of reactive oxygen species (ROS). The current work evaluated the protective activity of aqueous leaf extract of Costus afer (ALECA)) on liver damage arsing from exposure to trace metal mixture (TMM): cadmium chloride (CdCl2), lead chloride (PbCl2), and mercury chloride (HgCl2). Five groups of weight matched Sprague Dawley rats were treated for 90 days. Metal mixture and deionized water were used to treat the 2 groups of rats whereas the other 3 groups were treated with various doses of the ALECA through oral gavage alongside the metal mixture. Hepatic function parameters, oxidative biomarkers, inflammatory cytokines, Morphological changes and trace metals (concentrations were monitored in the liver). TMM treatment resulted in significant increase in ALT, AST, ALP, bilirubin, IL-6, MDA, but decreased albumin, total protein, IL-10, SOD, CAT and GSH levels. TMM also caused some morphological changes and increased the heavy metal (Pb, Cd and Hg) concentrations in the liver. The leaf extract gave a reasonable protective effect on the hepatotoxicity caused by trace metal mixture - through the mechanisms of metal chelation, anti-inflammatory, and antioxidant although this depends on the dosage to the rats. ALECA may be beneficial in the management of liver toxicity.

scholarly journals Effect of Organic and Botanicals Priming on Seed Quality Parameter of Pigeonpea (Cajanuscajan (L.) Millspaugh)

2021 ◽  
pp. 1-5
Author(s):  
A. Divij Reddy ◽  
A. K. Chaurasia ◽  
P. K. Shukla ◽  
Ram Pal Singh
Keyword(s):  
Growth Regulators ◽  
Leaf Extract ◽  
Seed Quality ◽  
Quality Parameters ◽  
Quality Parameter ◽  
The Other ◽  
Hard Seed ◽  
Normal Seedling ◽  
Seed Coats ◽  
Moringa Leaf Extract

Presence of hard seed coats and several seed borne pathogens hinder the normal seedling establishment in legumes. Earlier seed enhancement is mainly achieved though synthetics and growth regulators. To evaluate the effect of organics and botanicals on quality parameters of the seed an experiment was conducted in Sam Higginbottom University of Agriculture, Technology and Sciences. Treatments in the experiment comprised of Panchagavya at 3%, 5% and 7% for 12 hrs., Beejamrutha at 5%, 7% and 9% for 12 hrs., Coconut water at 6% and 8% for 12hrs., Moringa leaf extract at 5% and 7% for 12hrs and Sea weed extract at 3% and 5% for 12hrs.Priming with the selected organics and botanicalsis found to enhance the quality parameters compared to untreated control. The treatment Panchagavya at 7% for duration of 12 hours was found to be superior compared to the other treatments in the study.

scholarly journals Allelopathic Interactions of Parthenium hysterophorous with Kidney Bean, Phaseolus vulgaris

2020 ◽  
pp. 25-33
Author(s):  
H. M. Tsingalia ◽  
J. M. Auma
Keyword(s):  
Phaseolus Vulgaris ◽  
Seed Germination ◽  
Leaf Extract ◽  
Shoot Growth ◽  
Combined Treatment ◽  
Kidney Bean ◽  
Root Extract ◽  
Indigenous Species ◽  
Distilled Water ◽  
Leaf Extracts

Allelopathy, an important mechanism in invasive species stems from the lack of coevolved tolerance of indigenous species to novel chemicals released by the invader species. These chemicals are key to successful invasive of species of natural plant communities and ecosystems. This study analyzed the allelopathic effects of the invasive plant, Parthenium hysterophorous on the growth of kidney beans (Phaseolus vulgaris). One hundred and fifty viable kidney bean seeds were washed in distilled water, and transferred in triplicates to pots with wet sterile sand. Ten grams of freshly collected Parthenium leaves and roots were weighed, grounded separately using a mortar and a pestle, mixed with 100 ml of distilled water in a beaker and left for 24 hours in a dark at a room temperature (250C). The aqueous extract filtrates were used in experimental treatments. While untreated (control) seeds germinated on day 4 seeds treated with leaf extract germinated on day 14 and day 9 for the root extract. Leaf extract inhibited germination and sprouting of new leaves more effectively than root extract. Leaves in control seeds appeared on day 1 after germination. In treated seeds, leaves appeared on the 4th day then stagnated until day 15 when more leaves sprouted. Root extract significantly depressed seed germination. The number of new leaves increased significantly in control seedlings compared to the treated ones. Combined root and leaf extracts highly significantly inhibited of seed germination and the number of new leaves. Additionally, combined treatment with root and leaf extracts significantly inhibited shoot growth, and the increase in leaf length and width. A comparison of the effectiveness of the root and leaf extracts showed that leaf extract was more effective in inhibiting shoot growth and leaf width than the root extract.

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