Seed quality and plantlet traits of perennial ryegrass after 5 years of seed storage at various temperatures

Seed quality may be affected by several factors, including permeability, color, and lignin content in the seed coat. This study aimed at evaluating influence of lignin content in the tegument of seed samples of six different soybean cultivars, in which half of each sample was inoculated with the fungus Aspergillus flavus, on the physical and physiological quality, and on the seed health, during 180 days storage period, under cold chamber with controlled conditions of temperature and RH. For that, at each interval of 60 days, samples were removed, and the physiological quality of these seeds was assessed by means of moisture and lignin contents; and by tests of seed health, germination, and electrical conductivity. The moisture content of seeds remained constant during all storage period. In the seed health test, it was found that inoculation was efficient, once the minimum incidence of the fungus in the inoculated seeds was 85%. In the germination test, there was a trend of reduction on percentage germination with the increase in storage period. However, there was an increase on electrical conductivity of seeds assessed. It was concluded that there is no interference of the lignin content in the seed coat on the resistance to infection by the fungus Aspergillus flavus, even after seed storage for a period of 180 days.

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Pola Penurunan Viabilitas dan Pengembangan Metode Pendugaan Vigor Daya Simpan Benih Pepaya (Carica Papaya L.)

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.7.3.146-154 ◽

2017 ◽

Vol 7 (3) ◽

pp. 146

Author(s):

Astryani Rosyad ◽

M. Rahmad Suhartanto ◽

Abdul Qadir

Keyword(s):

Moisture Content ◽

Aging Time ◽

Seed Quality ◽

Initial Moisture Content ◽

Accelerated Aging ◽

Storage Period ◽

Seed Storage ◽

Seed Vigor ◽

Develop Model ◽

Papaya Seed

ABSTRACT Information of seed quality during storage can be determined through the actual storage and storability vigor estimation. This study aimed at comparing effective accelerated aging method between physical and chemical, and studying the seed deterioration during storage in ambient (T =28-30 0C, RH=75-78%) and AC (T =18-20 0C, RH =51-60%) condition with three levels of initial moisture content (8-10%, 10-12%, and 12-14%) for 20 weeks. The final objective of this research was to develop model for storability vigor of papaya seed. Two experiments, accelerated aging and seed storage were conducted at Seed Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University from October 2015 to May 2016. A completely randomized design with nested factors and four replications was applied to both experiments. The results showed that physical accelerated aging using IPB 77-1 MMM machine was more effective than chemical accelerated aging using IPB 77-1 MM machine for papaya seed. The viability of seed stored in AC condition remained high until the end of the storage period, whereas it declined at 16 week storage period in the ambient condition. The viability of seed with initial moisture content of 12-14% declined faster than that of initial moisture content of 8-10% after 18 week storage periode. The model used to estimate the storability vigor of papaya seed accurately was the equation y = a + b expcx where y : storability vigor estimation, x : aging time and a,b,c : constant value. Simulation of storability vigor estimation with constant value of a, b, c and input of aging time can estimate storability seed vigor in actual storage. Keywords: accelerated aging, IPB 77-1 MM machine, IPB 77-1 MMM machine, seed storage, simulationABSTRAK Informasi mutu benih selama penyimpanan dapat diketahui melalui penyimpanan secara aktual dan pendugaan vigor daya simpan. Penelitian ini bertujuan untuk membandingkan metode pengusangan cepat yang efektif antara fisik dengan kimia serta mempelajari pola penurunan viabilitas benih selama penyimpanan aktual pada kondisi simpan kamar (suhu =28-30 0C, RH =75-78%) dan AC (suhu =18-20 0C, RH =51-60%) dengan tiga tingkat kadar air awal (8-10%, 10-12%, dan 12-14%) selama 20 minggu. Tujuan akhirnya adalah membangun model vigor daya simpan benih pepaya. Penelitian pengusangan cepat dan penyimpanan dilakukan pada bulan Oktober 2015 sampai Mei 2016 di Laboratorium Benih, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor. Kedua penelitian menggunakan rancangan acak lengkap tersarang dengan empat ulangan. Hasil penelitian menunjukkan bahwa pengusangan cepat secara fisik dengan alat IPB 77-1 MMM lebih efektif daripada pengusangan kimia dengan alat IPB 77-1 MM untuk benih pepaya. Viabilitas benih yang disimpan pada kondisi AC tetap tinggi hingga akhir periode simpan, sedangkan pada kondisi kamar penurunan viabilitas dimulai pada periode simpan 16 minggu. Benih yang disimpan dengan tingkat KA awal sebesar 12-14% lebih cepat mengalami penurunan viabilitas mulai periode simpan 18 minggu dibandingkan dengan benih dengan KA awal 8-10%. Hasil penelitian juga menunjukkan terdapat korelasi yang erat antara pola kemunduran benih pada pengusangan cepat dan penyimpanan aktual, sehingga model pendugaan vigor daya simpan (y) berdasarkan waktu pengusangan (x) dapat disusun dengan persamaan y = a + b expcx. Simulasi pendugaan vigor daya simpan dengan nilai konstanta a, b, dan c serta input waktu pengusangan dapat menduga vigor daya simpan benih selama penyimpanan aktual. Kata kunci: alat IPB 77-1 MM, alat IPB 77-1 MMM, pengusangan cepat, penyimpanan benih, simulasi

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Impacts of wheat seed storage at poor household level in Bangladesh

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v43i2.37337 ◽

2018 ◽

Vol 43 (2) ◽

pp. 345-360

Author(s):

MA Monayem Miah ◽

QMS Islam ◽

ME Baksh ◽

FJ Rossi ◽

TP Tiwari

Keyword(s):

Seed Quality ◽

Seed Storage ◽

Net Income ◽

Wheat Seed ◽

Financial Benefit ◽

Household Level ◽

Level Of Satisfaction ◽

Wheat Cultivation ◽

Plastic Metal ◽

New Varieties

Small and marginal farmers have little access to improved seed from institutional sources and are thus largely excluded from the benefits of new varieties. The production and storage of improved varieties seeds at the household (HH) level can successfully overcome this problem. With this aim CSISA-CIMMYT (Cereal Systems Initiative for South Asia) project in Bangladesh have been working since 2012. Therefore, the study assessed the impacts of wheat seed storage systems at HH level, with a particular emphasis on how the poor farmers are benefited by doing the seed storage business. The study analyzed data and information collected at random from 210 supported and 60 non-supported farmers spread over three wheat growing districts namely Mymensingh, Faridpur and Rangpur. Wheat farmers used different storage containers and showed the highest level of satisfaction towards plastic sac along with poly bags and plastic/metal drum due to cost effectiveness and seed quality maintenance. On an average, supported and non-supported farmers retained respectively 103 kg and 100 kg of seed at household level, and sold most of their seeds to neighbouring farmers, local markets, and dealers. Wheat seed storage at household level was a profitable business to most of the respondent farmers. They could earn a reasonable net income (Tk.1127-Tk.1210) from seed storage. The farmers who stored seed in plastic/metal drum received the highest net income due to higher storage capacity, less storage cost, and higher seed price. The wheat storage program has created significant impacts in the study areas. A substantial increase was recorded in wheat area, wheat productivity, and financial benefit of the wheat farmers as a whole. Nevertheless, improved wheat seed is now available at farm level and most farmers become enthusiastic towards improved wheat cultivation because of this program. Respondent farmers did not face any critical problem during seed storage.Bangladesh J. Agril. Res. 43(2): 345-360, June 2018

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Evaluasi ketahanan simpan enam belas genotip benih jagung hibrida Unpad pada periode simpan empat bulan

Kultivasi ◽

10.24198/kultivasi.v17i1.15854 ◽

2018 ◽

Vol 17 (1) ◽

Cited By ~ 1

Author(s):

Anne Nuraini ◽

Sumadi Sumadi ◽

Muhamad Kadapi ◽

Agus Wahyudin ◽

Dedi Ruswandi ◽

...

Keyword(s):

Seed Quality ◽

Good Condition ◽

Storage Period ◽

Seed Storage ◽

Rapid Reduction ◽

Seed Technology ◽

Short Period ◽

Significant Difference ◽

Hybrid Maize ◽

Seed Storability

Abstract. Seed storage is a post-harvest activity that is done to maintain the seed quality before planting. The problem often encountered in seed storage is the rapid reduction of seed quality in short period of time. Seed storability is important to maintain seed quality in good condition. The aim of this research was to find out the best seed storability of 16 genotypes of UNPAD Hybrid Maize Seeds after 4 months storage period. This research was done by identificating best seed storability after some storage period of single hybrid maize seed, namely, DR (Downey Resistance), BR, MDR (mutant of DR), and MBR (mutant of BR) which are a collection of Maize Development Team Plant Breedung Laboratory Faculty of Agri-culture Universitas Padjadjaran. This research was conducted from October 2016 until February 2017 at Seed Technology Laboratory Faculty of Agriculture Universitas Padjadjaran. Completely Randomized Design was used and repeated two times. Data were tabulated and analyzed using the F test, while to test the significant difference further were using Scott Knott test level of 5%. The result showed that there is significant difference in electrical conductivity value, 1000 grain weight, seed germination capacity, vigor index, seed growth simultaneously, and normal seedling dry weight after 4 months storage period. MDR 18.5.1, DR 17, 4BR 157, 4MDR 14.1.1 were the best genotype on seed storability after 4 months storage period.Keywords : maize, genotypes, seed storability, storage period Sari. Penyimpanan benih merupakan kegiatan pascapanen yang dilakukan untuk memper-tahankan mutu benih hingga benih tersebut siap ditanam. Permasalahan yang sering dihadapi pada penyimpanan benih yaitu penurunan mutu benih secara cepat dalam periode yang belum terlalu lama. Tujuan dari penelitian ini adalah untuk mengetahui ketahanan simpan 16 genotip benih jagung hibrida UNPAD yang terbaik setelah periode simpan empat bulan. Penelitian ini dilakukan dengan mengiden-tifikasi ketahanan simpan terbaik setelah bebe-rapa periode simpan dari genotip benih jagung hibrida tunggal, yaitu genotip DR (Downey Resistance), BR, MDR (mutan DR), dan MBR (mutan BR) yang merupakan koleksi Tim Pengembangan Jagung Laboratorium Pemuliaan Tanaman Fakultas Pertanian Universitas Padja-djaran. Penelitian dilakukan pada bulan Oktober 2016 sampai Februari 2017 di Labora-torium Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Penelitian ini dilakukan dengan menggunakan Rancangan Acak Leng-kap (RAL) 2 ulangan. Data dianalisis mengguna-kan uji F, sedangkan perbedaan yang signifikan antar perlakuan digunakan uji Scott Knott pada taraf nyata 5%. Hasil penelitian menunjukkan terda-pat perbedaan yang signifikan pada parameter daya hantar listrik, bobot 100 butir, daya berkecambah, indeks vigor, keserempakan tum-buh, serta bobot kering kecambah normal setelah periode simpan 4 bulan. Genotip MDR 18.5.1, DR 17, 4BR 157, 4MDR 14.1.1 merupakan genotip yang memiliki ketahanan simpan setelah periode simpan 4 bulan.Kata kunci: jagung, genotip, ketahanan simpan, periode simpan

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Survival of endophyte-infected ryegrass seed buried in soil

NZGA: Research and Practice Series ◽

10.33584/rps.7.1999.3391 ◽

1999 ◽

Vol 7 ◽

pp. 151-156

Author(s):

D.E. Hume ◽

M.P. Rolston ◽

D.B. Baird ◽

W.J. Archie ◽

M.R. Marsh

Keyword(s):

Lolium Perenne ◽

Perennial Ryegrass ◽

Seed Viability ◽

Seed Storage ◽

Fungal Endophyte ◽

Grass Species ◽

Soil Conditions ◽

Neotyphodium Lolii ◽

Viable Seeds ◽

One Year

Emergence of volunteer perennial ryegrass (Lolium perenne L.) from seed buried in soil may contribute to the ingress of ryegrass in newly sown pastures. To investigate this, ryegrass seed infected with fungal endophyte (Neotyphodium lolii) was buried in nylon bags under pasture at two depths and at two sites (Palmerston North, Lincoln) in early/mid-autumn 1998. Seed bags were removed from the soil at intervals over the course of one year to determine seed viability and presence of endophyte in seedlings. Viability of seed declined rapidly to be 10% 3 months after burial. Further decline in viability was less, so that 12 months after burial 4% of seeds were still viable. Endophyte viability also declined, from 58% infection of seedlings at the time of burial to 21% at 12 months. This was at a slower rate than the decline in seed viability and from what might have been predicted from seed storage experiments. Viability of seed buried at 10 cm was greater than that buried at 3 cm (e.g., means, 10% and 1% after 6 months, respectively). This has implications for cultivation practices before pasture establishment. Seed buried at Lincoln maintained higher viability than seed buried at Palmerston North (e.g., means, 6% and 4% after 6 months, respectively), which was associated with drier soil conditions at Lincoln. Survival of buried seed may therefore be of greater importance in summer-dry east coast regions, compared with moist west coast environments or in wet years. The significance of buried ryegrass seed will depend on the numbers involved, but after 12 months there were still viable seeds left in the soil and some of these were infected with endophyte. This is important for pastures sown with ryegrass that is free of endophyte or infected with a selected endophyte, and for slower establishing grass species such as tall fescue. Keywords: endophyte, endophyte survival, Lolium perenne, Neotyphodium lolii, perennial ryegrass, seed burial, seed survival

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Moisture adsorption isotherms and quality of seeds stored in conventional packaging materials and hermetic Super Bag

10.1101/463026 ◽

2018 ◽

Author(s):

Muhammad Amir Bakhtavar ◽

Irfan Afzal ◽

Shahzad Maqsood Ahmed Basra

Keyword(s):

Adsorption Isotherms ◽

Seed Quality ◽

Storage Period ◽

Seed Storage ◽

Packaging Materials ◽

Seed Moisture Content ◽

Moisture Adsorption ◽

Moisture Contents ◽

Seed Moisture

AbstractSeed moisture content (SMC) is an important attribute to seed quality. Maintaining seed dryness throughout supply chain (The Dry Chain) prevents seed germination and quality losses. Ambient relative humidity (RH) and temperature affect seed moisture and thereof seed moisture isotherm. Present study was conducted to compare the moisture adsorption isotherms of wheat, maize, cotton and quinoa seeds packed in hermetic Super Bag and traditional packaging materials including paper, polypropylene (PP), jute and cloth bags. Seeds were incubated at 60, 70, 80 and 90% static RH. Nearly straight line moisture isotherms for all crop seeds were obtained in Super Bag. Seed moisture contents increased in traditional packaging materials with increasing RH. At higher level of RH, moisture contents increased slightly (1-2%) in Super Bag, whereas this increase was much higher in traditional packaging materials (≈9% higher than original SMC at 90% RH). In second study, seeds were dried to 8 and 14% initial seed moisture contents using zeolite drying beads and were stored in hermetic and traditional bags for a period of 18 months. For all crop seeds, germination was severely affected in all packaging materials both at 8 and 14% initial SMC except storage in Super Bag at 8% SMC. Wheat seed stored in Super Bag at 8% SMC almost maintained initial germination while germination of cotton, maize and quinoa seeds declined 7%, 14% and 30% respectively in Super Bag at 8% SMC. Seed storage in Super Bag can help to prevent the significant increase in seed moisture at higher RH as is evident from moisture isotherm study, thus helps to preserve quality of maize, wheat, cotton and quinoa seeds by maintaining The Dry Chain throughout the storage period.

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EFFECT OF STORAGE CONTAINERS AND SEED TREATMENTS ON SEED GERMINATION AND VIGOUR OF BLACK GRAM (VIGNA MUNGO (L.) HEPPER)

PLANT ARCHIVES ◽

10.51470/plantarchives.2021.v21.s1.386 ◽

2020 ◽

Vol 21 (supplement 1) ◽

Author(s):

J.Johnny subakar ivin ◽

Y.Anbuselvam subakar ivin ◽

M.Surendhar J.R. Jerish ◽

M.Surendhar J.R. Jerish

Keyword(s):

Shelf Life ◽

Seed Quality ◽

Seed Viability ◽

Seed Storage ◽

Wire Mesh ◽

Monthly Interval ◽

Black Gram ◽

Neem Oil ◽

The Poor ◽

Vigour Index

One of the most important basic needs for higher agricultural production is quality seed, characterized by high viability and vigour. Maintenance of seed viability and vigour from Harvest till the next growing season is of the utmost importance in a seed production programme. During seed storage, qualitative and quantitative losses of up to 20% have been reported in India. The poor seed quality may also be due to the poor storability which is very often being decided by the internal and external factors. In pulses, the major cause for seed deterioration during storage is bruchid damage. In this context, evolving an improved storage strategy to prolong the shelf life of seeds under ambient storage conditions with easily available cost-effective resources was carried out. The experiment was conducted at the Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Annamalai nagar. Freshly harvested seeds of black gram cv. ADT 3 were cleaned and graded using BSS, 8x8 wire mesh sieve. Then seeds were dried under sunlight to bring the moisture content to required level (8.5%). The seeds were treated with Thiram @ 2g kg-1, Neem oil @ 10ml kg-1 and Malathion dust @ 200 mg kg-1. Two hundred and fifty grams of seeds from all treatments along with control were packed in two containers viz. cloth bag and polythene bag (700 gauges) with three replication and stored in at ambient temperature (30o c – 35o c) and relative humidity of 52-80%. Seed samples were drawn from each replication at monthly interval for assessing the viability and vigour. Germination test was conducted in between paper method and seedlings were evaluated on seventh day for vigour index bruchid infestation. The untreated seeds recorded lower germination per cent and vigour index after seven months of storage. There was no bruchid damage on seeds treated with neem oil @ 10ml kg-1 and thiram @ 2g kg-1. It was concluded that the shelf life of black gram seeds could be increased by

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Influence of abiotic factors on seed quality of maize hybrids in the process of its storage

The Scientific Journal Grain Crops ◽

10.31867/2523-4544/0155 ◽

2021 ◽

Vol 5 (1) ◽

pp. 30-37

Author(s):

M. Ya. Kyrpa ◽

Yu. S. Bazilieva ◽

O. F. Stasiv

Keyword(s):

Growth Rate ◽

Moisture Content ◽

Chemical Treatment ◽

Seed Quality ◽

Abiotic Factors ◽

Seed Mass ◽

Seed Storage ◽

Maize Hybrids ◽

Storage Methods

The research results of influence of various abiotic factors on seed quality of maize hybrids during long storage are given. The most effective of them are: temperature regime, seed moisture content and oxygen access. The storage methods (dry and cold, and controlled atmosphere storage) that have the greatest impact on the seed quality were established. It was found that the optimal moisture content of seed depends on the storage duration, if for 1–2 years – 10–11 %, 3 years and more – 7–8 %. In order to stabilize the moisture content, it is recommended to store the seeds in airtight containers, limiting the humidity access from the outside. During the long-term seed storage, particularly for the breeding purposes, the temperature range should advisably maintain within 8–10 °C in seed storage place. With that the laboratory germination of seeds increased by 3–5 %, field germination – by 6–8 %, growth rate by number of sprouts– by 5–9 % and by the weight of sprouts – by 2–5 g compared to the 18–20 °C temperature in a typical storage. Methods of pre-sowing improvement of seed quality were established – it is separation and chemical treatment (seed dressing). Separation is the sifting of the seed mass through sieves with round holes in order to divide from the smallest fraction, the content of which was 15–23 % depending on the seed uniformity of maize hybrids. It was found that in order to assess objectively the effectiveness of different chemicals and establish their suitability for presowing seed treatment, it is necessary to determine laboratory germination, especially the growth rate by the number of sprouts and weight of 100 sprouts. Premature chemical treatment of seed should be avoided. Key words: maize hybrids, storage methods, abiotic factors, seed quality, presowing treatment.

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Principles and methods of standardization in seed quality control

сборник статей всероссийской научной конференции с международным участием "Растениеводство и луговодство" ◽

10.26897/978-5-9675-1762-4-2020-6 ◽

2020 ◽

Author(s):

S.N. Volkova S.N. ◽

◽

E.E. Sivak E.E.

Keyword(s):

Quality Control ◽

Seed Quality ◽

Seed Storage ◽

High Quality ◽

Seed Material ◽

Improve Production ◽

Part Time ◽

High Quality Seed ◽

Seed Crops ◽

The Way

Compliance with agronomic regulations in farms that ensure high quality seeds (conducting grade and species weeds on seed crops, compliance with harvesting, part-time, seed storage, etc.) must be constantly monitored in order to improve production on the way to the cultivation of high-quality seed material.

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Seed survival in Chilean Nothofagus in response to desiccation and storage

Seed Science Research ◽

10.1079/ssr2005204 ◽

2005 ◽

Vol 15 (2) ◽

pp. 113-123 ◽

Cited By ~ 4

Author(s):

Pedro León-Lobos ◽

Richard H. Ellis

Keyword(s):

Moisture Content ◽

Seed Quality ◽

Seed Viability ◽

Seed Storage ◽

Forest Tree ◽

Ex Situ ◽

Hermetic Storage ◽

Moisture Contents ◽

Tree Seeds ◽

Initial Seed

Nothofagus alpina,N. obliqua,N. glauca,N. leonii,N. dombeyiandN. pumilioseeds exhibited consistent, albeit slight, sensitivity to extreme desiccation, but nevertheless maintained viability at low moisture contents and cool temperatures (–10° to –20°C) over 2 years.Nothofagus alpina,N. obliqua,N. glauca,N. leoniiandN. dombeyiconformed to the seed viability equation of Ellis and Roberts; sensitivity of longevity to temperature was quantitatively similar to that of crop seeds, sensitivity to moisture was somewhat less, and a low-moisture-content limit to the equation was detected at 4.8% moisture content in hermetic storage at 65 °C, and possibly similar moisture contents at 30–40°C. These five species show orthodox seed storage behaviour. Therefore,ex-situconservation of theseNothofagusspecies in seed banks is possible, but the quality of seed lots collected requires attention. Seed storage behaviour was not defined inN. pumilio: initial seed quality was poor and loss of viability was detected over 2 years at 0°, –10° and –20°C at 2.7% moisture content, but not at 5.2%. The results confirm that the economy of nature in seed storage physiology extends to forest tree seeds, but the repeated observation of reduced sensitivity of longevity to moisture in forest tree seeds requires further investigation.

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