scholarly journals Pre-Exposure to Dennettia tripetala Ethanolic Fruit Extract Prevents Biochemical Alterations in Rats Subsequently Exposed to a Single Dose of Carbon Tetrachloride

2017 ◽  
Vol 6 ◽  
pp. 8-16 ◽  
Author(s):  
Sylvia O. Iseghohi ◽  
Noghayin E.J. Orhue ◽  
Kingsley Omage
Keyword(s):  
Carbon Tetrachloride ◽  
Kidney Injury ◽  
Ethanol Extract ◽  
Albino Rats ◽  
Albumin Concentration ◽  
Biochemical Alterations ◽  
Dennettia Tripetala ◽  
Liver And Kidney ◽  
Pre Treatment

Dennettia tripetala(DT) is consumed in West Africa as a spice. It is also used in traditional medicine for treating cough, fever and other ailments. Its fruits have been shown to possess phytochemicals with proven antioxidant capabilities. DT roots elicitin vitroantioxidant capabilities similar to that of ascorbic acid. In this study, we evaluated the potency of the ethanol extract of DT fruits in preventing liver damage induced by a single oral administration of Carbon tetrachloride (CCl4). Thirty female Wistar albino rats were randomized into six groups of five animals each: Group A served as control, Groups B-D were given increasing doses (250, 500 and 1000mg/kg b.w respectively) of DT extract for 14 days by gavage; on day 15, they were given CCl4(3ml/kg bw) by gavage. Group E was given the highest dose of DT for 14 days without CCl4while Group F was given only CCl4on day 15. Administration of CCl4resulted in liver and kidney injury detected as significant increases in plasma ALT, AST, ALP and GGT activities as well as plasma total protein, urea, creatinine, plasma and liver triglyceride and cholesterol as well as liver and kidney malondialdehyde concentrations. CCl4also caused a reduction in liver and kidney SOD and catalase activities as well as plasma albumin concentration. Pre-treatment withDennettiatripetalahowever, significantly prevented damage to the liver and kidney. Under the conditions of this study,Dennettia tripetalashows potential in preventing liver and kidney damage and has no noticeable side effects.

Hepato- and Nephroprotective Effects of the Polyphenol Concentrate From Cabernet Sauvignon Grape Varieties Cultivated in Kazakhstan in the Experimental Model Of CCL4-Induced Toxicity

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Nurgozhin T. ◽  
Sergazy S. H. ◽  
Adilgozhina G. ◽  
Gulyayev A. ◽  
Shulgau Z. ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Experimental Model ◽  
Lethal Dose ◽  
Radical Scavenging ◽  
Cabernet Sauvignon ◽  
Intragastric Administration ◽  
Liver And Kidney ◽  
Antioxidant Stress

Objective:This study investigates the hepatoprotective effect and the antioxidant role of polyphenol concentrate in the experimental model of carbon tetrachloride (CCl4) induced toxicity. Methods: Antioxidant activity of Cabernet Sauvignon grape polyphenol were evaluated by radical scavenging of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), 2,2’-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS.+). In addition, the effects of polyphenol concentrate on the survival of Wistar rats in the toxicity model, was also investigated. The polyphenol concentrate was administered for 5 five days prior to injection of carbon tetrachloride in a sub-lethal dose of 300 mg/kg of animal body weight in order to perform histological examinations of the liver and kidney, and detect the levels of AST, ALT and bilirubin. Results: Administration of polyphenol concentrate increased animal survival in the experimental model. Moreover, the intragastric administration of polyphenol concentrate prior to the initiation of the experimental model of toxicity, which was caused by a sub-lethal CCl4 dose, reduced morphological injuries in the liver and kidney, decreased the AST and ALT levels of the blood serum. Discussion and conclusion: Our data demonstrate that polyphenol concentrate possesses an antioxidant potential both in vitro and in vivo by reducing antioxidant stress that was caused by CCl4 administration into rats.

scholarly journals Protective Effect of Eckol against Acute Hepatic Injury Induced by Carbon Tetrachloride in Mice

Marine Drugs ◽  
2018 ◽  
Vol 16 (9) ◽  
pp. 300 ◽  
Author(s):  
Shulan Li ◽  
Juan Liu ◽  
Mengya Zhang ◽  
Yuan Chen ◽  
Tianxing Zhu ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Protective Effect ◽  
Acute Liver Injury ◽  
Treated Group ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Enhanced Expression ◽  
Pre Treatment

Several in vitro studies have shown the potential hepatoprotective properties of eckol, a natural phlorotannin derived from the brown alga. However, the in vivo hepatoprotective potential of eckol has not been determined. In this study, we performed an in vivo study to investigate the protective effect of eckol and its possible mechanisms on the carbon tetrachloride (CCl4)-induced acute liver injury model in mice. Results revealed that eckol pre-treatment at the dose of 0.5 and 1.0 mg/kg/day for 7 days significantly suppressed the CCl4-induced increases of alanine transaminase (ALT) and aspartate aminotransferase (AST) levels in serum and meliorated morphological liver injury. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) analysis showed that the number of positive apoptotic hepatocytes in the eckol-treated group was lower than that in the CCl4 model group. Western blotting analysis also demonstrated the enhanced expression of bcl-2 and suppressed expression of cleaved caspase-3 by eckol. The CCl4-induced oxidative stress in liver was significantly ameliorated by eckol, which was characterized by reduced malondialdehyde (MDA) formations, and enhanced superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and glutathione (GSH) content. Moreover, the CCl4-induced elevations of pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 were markedly suppressed in the eckol-treated group. However, eckol enhanced the level of IL-10, a potent anti-inflammatory cytokine, and recruited CD11c+ dendritic cells into the liver tissues of CCl4-treated mice. These results indicated that eckol has the protective effect on CCl4-induced acute liver injury via multiple mechanisms including anti-apoptosis, anti-oxidation, anti-inflammation and immune regulation.

scholarly journals In vitro Antioxidant and In vivo Hepatocurative and Nephrocurative Activities of Aqueous Leaf Extract of Newbouldia laevis in Albino Rats

2021 ◽  
pp. 114-125
Author(s):  
Mohammed A. Sulaiman ◽  
Mahmoud S. Jada ◽  
Augustine Elizabeth ◽  
Abubakar Umar Modibbo
Keyword(s):  
Oral Administration ◽  
Leaf Extract ◽  
Kidney Diseases ◽  
Albino Rats ◽  
Protein Levels ◽  
Ameliorative Effect ◽  
Liver And Kidney ◽  
Aqueous Leaf Extract

The in vitro antioxidant activity and in vivo hepatocurative and nephrocurative potential of Newbouldia laevis aqueous leaf extract (NLALE) was evaluated. The study used 30 male, albino rats (Rattus norvegicus) weighing 180 ± 20 g, of which 25 were intoxicated by oral administration of a single dose of diclofenac (100 mg/kg b. wt.). Animals were treated by oral administration of silymarin (200 mg/kg b. wt.), furosemide (1.5 mg/kg b. wt.) and NLALE (200 mg/kg and 400 mg/kg b. wt.) for seven consecutive days before animals were sacrificed on the 8th day and serum/plasma was analyzed for biochemical markers of hepatotoxicity and nephrotoxicity. Phytochemical screening of NLALE revealed the presence of alkaloids, flavonoids, glycosides, phenols, saponins, steroids and tannins. The extract scavenged DPPH radical, reduced Fe3+ and inhibited TBARs in comparable manner to ascorbic acid in vitro. NLALE also attenuated diclofenac-induced liver and kidney intoxication as indicated by the significantly (p<0.05) reduced levels of serum biomarkers of hepatotoxicity: ALT, AST, bilirubin, but increased total protein levels and nephrotoxicity: urea, creatinine, Na+ and K+. The observed effects are dose dependent as the 400 mg/kg b. wt. appeared to be more potent than the 200 mg/kg b. wt. dose. It may be concluded from this study that Newbouldia laevis leaf has ameliorative effect against diclofenac-induced hepatotoxicity and nephrotoxicity probably through antioxidative mechanism and the curative claim and the folkloric use of the plant in the treatment of liver and kidney diseases have been scientifically validated

scholarly journals Salicornia ramosissima J. Woods seeds affected the normal regenerative function on carbon tetrachloride-induced liver and kidney injury

2018 ◽  
Vol 107 ◽  
pp. 283-291 ◽  
Author(s):  
Daniela Ferreira ◽  
Diana C.G.A. Pinto ◽  
Helena Silva ◽  
Ana Paula Girol ◽  
Maria de Lourdes Pereira
Keyword(s):  
Carbon Tetrachloride ◽  
Kidney Injury ◽  
Salicornia Ramosissima ◽  
Liver And Kidney

In vitro antioxidants and hepatoprotective effects of Pleurotus tuber-regium on carbon tetrachloride–treated rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Kenneth O. Okolo ◽  
Orish. E. Orisakwe
Keyword(s):  
Gas Chromatography ◽  
Carbon Tetrachloride ◽  
Ethanol Extract ◽  
Liver Function Tests ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Oxidative Stress Biomarkers ◽  
Group I ◽  
Hepatoprotective Effects

AbstractObjectivesThis study has characterized the phytoconstituents and evaluated the in vitro antioxidant and hepatoprotective effects of Pleurotus tuber-regium induced by carbon tetrachloride (CCl4). In vitro antioxidant assay of ethanol extract of P. tuber-regium and gas chromatography-mass spectrometry analyses to identify the phytoconstituents were carried out.MethodsSixty rats were divided into six groups of 10 animals in each group and treated as follows for 13 weeks. Group I (control) received 3 mL/kg olive oil intraperitoneal twice weekly in addition to feed and water ad libitum. Group II received CCl4 3 mL/kg twice weekly. Groups III, IV and V received 100, 200 and 500 mg/kg wild edible P. tuber-regium mixed with feed by ingestion daily in addition to 3 mL/kg CCl4 twice weekly, respectively. Group VI received 500 mg P. tuber-regium daily. Liver and body weights were recorded. Liver function tests, oxidative stress biomarkers, bilirubin, ascorbic acid and α-tocopherol were assayed. Histopathology of the liver was carried out. The gas chromatography-mass spectroscopy analysis yielded 10 antioxidants.ResultsCCl4 increased the levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, bilirubin and malondialdehyde from 24±1.778 iu/L, 53±3.7 iu/L, 257±19 iu/L, 0.45±0.03 mg/dL and 1.90±0.42 μmol/L in the control group to 48±2.5 iu/L, 81±2.10 iu/L, 495±38 iu/L, 1.20±0.09 mg/dL and 14.0±2.6 μmol/L in the treated group, respectively.ConclusionsP. tuber-regium prevented the necrosis, edema and vein congestion observed in the CCl4-only group. P. tuber-regium is effective in protecting the liver against CCl4-induced damage.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY, DIURETIC AND HYPOGLYCEMIC ACTIVITIES OF ETHANOL EXTRACT FROM LEAF OF CHRYSOPHYLLUM ALBIDUM G. IN WISTAR ABINO RATS

2018 ◽  
pp. 16
Author(s):  
Olorunshola Dave Omodamiro ◽  
Chinedu Nwankwo
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Fasting Blood Glucose ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Albino Rats ◽  
Scavenging Activity ◽  
Standard Drug ◽  
Medicinal Uses

Chrysophyllum albidum G. is generally regarded as a plant with many ethno-medicinal uses. The study was aimed at evaluating the in vitro antioxidant, diuretic, and hypoglycemic properties of the ethanol extract of leaves of C. albidum Male Wistar albino rats weighing between 150g and 200g were used for the experiment. The DPPH scavenging activity was determined using DPPH assay, inhibition of lipid peroxidation was carried out using the reactions of peroxides with thiobarbituric acid, while nitric oxide was conducted based on the Greiss reaction. Diuretic activity was carried out using the method of Lipschitz with slight modifications. Alloxan (140 mg/kg) was used to induce diabetes in the rats. The extract showed a concentration-dependent increase in DPPH radical scavenging activity, anti-lipid peroxidation activity, and nitric oxide inhibition activity maximal at 200 mg/ml (84.99±7.97, 79.82±6.10, 69.99±2.22 respectively) and this was significant (p<0.05) when compared with that of 100 mg/ml of vitamin C (97.68±0.84, 99.05±0.24 and 98.15±0.29 respectively) which served as the control. The extract also significantly (p<0.05) increased; urine volume, urinary sodium, and urinary chloride particularly at 2000 mg/kg (1.55±0.07, 109.60±0.57, and 106.00±1.41 respectively) when compared with the standard drug (1.85±0.07, 115.20±0.57, and 112.85±0.49 respectively). The increase in potassium excretion was also significant (p<0.05) particularly at 2000 mg/kg (13.70±0.28) but less than those of sodium (109.60±0.57) and chloride (106.00±1.41) at the same concentration. There was no significant (p<0.05) increase in urinary bicarbonate the maximum being at 250 mg/kg (22.55±0.78). The extract caused a non-significant (p<0.05) decrease in the fasting blood glucose level of the rats. It was concluded that the leaves of C. albidum could be used as a source of natural antioxidant boosters and also a diuretic.

scholarly journals In vivo and in vitro antidiabetic properties of alkaloids extract from Salvia chudaei

2021 ◽  
pp. 45-53
Author(s):  
Mohammed Tlili ◽  
Roukia Hammoudi ◽  
Mahfoud Hadj-Mahammed
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Kidney Function ◽  
Inhibitory Effect ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Liver And Kidney ◽  
Mean Blood Glucose

The aim of this study was to evaluate, for the first time, the antidiabetic effect of the alkaloids extract of Salvia chudaei Batt. & Trab. (Lamiaceae) on alloxan-induced diabetic rats. The alkaloids extract was prepared, and the in vitro inhibitory effect of key digesting enzymes related to postprandial hyperglycemia were determined. After acute toxicity test, the Swiss albino rats were induced with alloxan to get experimental diabetes animals. The fasting mean blood glucose, lipid profile, different liver and kidney function biomarkers and antioxidant biomarkers levels, after treatment for 30 days, diabetic untreated and diabetic rats treated with alkaloids extract were estimated. The alkaloids displayed remarkable in inhibiting ?-glucosidase (IC50 = 248.25?2.61 ?g/ml) than ?-amylase (IC50 = 262.96?9.64 ?g/ml) activities. In vivo, the results proved that alkaloids extract at dose of 500 mg/kg bw decreased significantly the blood glucose, lipid profile levels and improved the liver and kidney function biomarkers and increased the activities of antioxidant enzymes (superoxide dismutase and gluthatione reductase). This study demonstrates, that alkaloids are effective in inhibiting hyperglycemia and oxidative stress caused by diabetes.

scholarly journals EFFECT OF ARTHROSPIRA PLATENSIS AS A FOOD SUPPLEMENT AGAINST SODIUM FLUORIDEINDUCED INTOXICATION ON SOFT TISSUES OF MALE WISTAR ALBINO RATS

2017 ◽  
Vol 10 (11) ◽  
pp. 133
Author(s):  
Ravindra Reddy Kandula ◽  
Narasimha Jayaveera Korlakunta ◽  
Raghavendra Mitta
Keyword(s):  
Body Weight ◽  
Soft Tissues ◽  
Radical Scavenging ◽  
Arthrospira Platensis ◽  
Albino Rats ◽  
Hydroalcoholic Extract ◽  
Group I ◽  
Liver And Kidney ◽  
Wistar Albino Rats

  Objective: The objective of the present study was to explore the in vitro antioxidant and effect of hydroalcoholic extract of Arthrospira platensis (ASP) against sodium fluoride (NaF)-induced toxic effects on soft tissues (heart, liver, and kidney).Methods: In vitro antioxidant activity was assessed using 1,1-diphenyl-2-picrylhydrazyl radical scavenging, 2,2’-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) radical scavenging, metal chelation, total antioxidant and reducing power assays, and total flavonoid and phenol content. In this study, 36 male Wistar albino rats were divided into six groups of six animals each. Animals were served as Group I - normal control, Group II - toxic control, and Groups III, IV, and V - treatment groups, which received hydroalcoholic extract of ASP at doses of 100, 200, and 400 mg/kg body weight (p.o), respectively. Group VI served as plant control received hydroalcoholic extract of ASP at a dose of 400 mg/kg body weight (p.o). All groups except Groups I and VI received NaF (100 ppm) through drinking water for 30 days.Results: Results showed that intoxication of NaF caused significant elevation of serum biomarkers of the heart, liver, and kidney and altered tissue oxidative stress markers’ levels. Administration of hydroalcoholic extract of ASP significantly normalized elevated serum levels of heart (p<0.001), liver (p<0.001, p<0.05), and kidney biomarkers (p<0.001). Decrease in lipid peroxidation and increase in reduced glutathione and catalase enzyme levels in a dose-dependent manner were observed in soft tissues (p<0.001, p<0.01, and p<0.05).Conclusion: The study revealed that ASP has good antioxidant and mitigative action against NaF intoxication on soft tissues.

scholarly journals Propolis Prevents Hepatorenal Injury Induced by Chronic Exposure to Carbon Tetrachloride

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Monika Bhadauria
Keyword(s):  
Oxidative Stress ◽  
Alkaline Phosphatase ◽  
Carbon Tetrachloride ◽  
Ethanolic Extract ◽  
Protective Role ◽  
Sprague Dawley ◽  
Chronic Injury ◽  
Biochemical Alterations ◽  
Propolis Extract ◽  
Liver And Kidney

Carbon tetrachloride (CCl4) is a well-known hepatotoxicant, and its exposure induces hepatorenal injury via oxidative stress and biochemical alterations. This study had been conducted to confirm the protective role of propolis extract on CCl4-induced hepatorenal oxidative stress and resultant injury. Propolis extracts collected from Gwalior district and 24 female Sprague Dawley rats were used for experiment. Animals were exposed to CCl4(0.15 mL/kg, i.p.) for 12 weeks (5 days/week) followed by treatment with propolis extract (200 mg/kg, p.o.) for consecutive 2 weeks. CCl4exposure significantly depleted blood sugar and hemoglobin level and raised the level of transaminases, alkaline phosphatase, lactate dehydrogenase, protein, urea, albumin, bilirubin, creatinine, triglycerides, and cholesterol in serum. Lipid peroxidation was enhanced, whereas GSH was decreased significantly in liver and kidney in CCl4-intoxicated group. Ethanolic extract of propolis successfully prevented these alterations in experimental animals. Activities of catalase, adenosine triphosphatase, glucose-6-phosphatase, acid, and alkaline phosphatase were also maintained towards normal with propolis therapy. Light microscopical studies showed considerable protection in liver and kidney with propolis treatment, thus, substantiated biochemical observations. This study confirmed hepatoprotective potential of propolis extract against chronic injury induced by CCl4by regulating antioxidative defense activities.

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