scholarly journals Strategies of Reducing the Toxicity of Sugar Mill Effluent by Using Biofertilizer Inoculants

2015 ◽  
Vol 32 ◽  
pp. 11-18 ◽  
Author(s):  
M. Rajesh ◽  
K. Natarajan ◽  
N. Renish
Keyword(s):  
Growth And Yield ◽  
Tagetes Erecta ◽  
Morphological Parameters ◽  
Sugar Mill ◽  
Culture Experiment ◽  
Sugar Mill Effluent ◽  
Effluent Irrigation ◽  
Set Up ◽  
Pot Culture

The present investigation has been carried out on Tagetes erecta to search out the effect of sugar mill effluent on growth and yield. In-vivo conditions were set up for the experiment purpose. Pots were filled with soil and treated with different concentrations of sugar mill effluent i.e., 10, 25, 50, 75 and 100% on morphological parameters of test crop. Results of pot culture experiment showed that at 10 % concentrations of sugar mill effluent proved to better to the crops growth and thereafter the growth may reduce over control. In order to mitigate the effluent toxicity certain Biofertilizers inoculants were mixed to the soil along with the same effluent treatments. The study suggests that the Biofertilizers inoculants along with effluent irrigation proved to be better for plants growth.

scholarly journals Various Biochemical Changes of African Marigold Seedlings under Sugar Mill Effluent Stress

2016 ◽  
Vol 50 ◽  
pp. 18-22
Author(s):  
T. Vaithiyanathan ◽  
P. Sundaramoorthy
Keyword(s):  
Developing Countries ◽  
Environmental Pollution ◽  
Rural Economy ◽  
Photosynthetic Pigment ◽  
Biochemical Changes ◽  
Tagetes Erecta ◽  
Sugar Mill ◽  
Sugar Production ◽  
Sugar Mill Effluent ◽  
African Marigold

Sugar mill is one of the agro based industry and great significant in rural economy of developing countries. It creates environmental pollution by produced waste during sugar production. In the present work deals with the analysis of various change of biochemical in African marigold (Tagetes erecta L.) at 15 DAS under the different concentrations (control, 10, 25, 50, 75 and 100%) of sugar mill effluent. Results of this study determined that the lower concentration (10%) of sugar mill effluent increased photosynthetic pigment and biochemical contents of African marigold and it decreased at higher concentrations of the sugar mill effluent. The lower concentrations of sugar mill effluent used for irrigation.

scholarly journals The effect of sugar mill effluent on seed germination of green gram (Vigna radiatia L.)

2016 ◽  
Vol 1 (1) ◽  
pp. 20 ◽  
Author(s):  
T. Vaithiyanathan ◽  
P. Sundaramoorthy
Keyword(s):  
Sugar Industry ◽  
High Growth ◽  
Inorganic Materials ◽  
Green Gram ◽  
Growth And Yield ◽  
Sugar Mill ◽  
Sugar Production ◽  
Sugar Mill Effluent ◽  
Living Organisms ◽  
Germination And Growth

Sugar industry is an agro-based industry in India which released lot of effluent into the environment during sugar production. The formers used this effluent in agriculture for scarcity of water. The effluent contained large amount of organic and inorganic materials which affects the water, soil and living organisms especially plants. The present study was the analysis of sugar mill effluent and different concentrations (10, 25, 50, 75 and 100%)  of sugar mill effluent effects on germination and growth development of green gram (Vigna radiata L.). As a result the lower concentration (10%) of sugar mill effluent promoted the germination and growth of the seedlings but higher concentrations of sugar mill effluent inhibited the germination and growth of the green gram seedlings. The lower concentration of effluent contained may be better nutrients of plant which used for irrigation purposed for high growth and yield.

The effect of ammonium sulphite on plant growth

1933 ◽  
Vol 23 (1) ◽  
pp. 1-5
Author(s):  
A. H. Lewis ◽  
F. B. Marmoy
Keyword(s):  
Adverse Effect ◽  
Plant Growth ◽  
Dry Matter ◽  
Growth And Yield ◽  
Review Of The Literature ◽  
Culture Experiment ◽  
Conflicting Evidence ◽  
Heavy Loam ◽  
Loam Soil ◽  
Pot Culture

Summary and ConclusionsA review of the literature gave conflicting evidence as to the effect of sulphites on plant growth, so that a pot culture experiment was conducted to study the effects of the addition to ammonium sulphate of varying amounts of ammonium sulphite on the germination, growth and yield of mustard and rye on a heavy loam soil.Under the conditions of the experiment described, ammonium sulphite had no adverse effect on the germination, growth and yield of dry matter of mustard or rye.

Continuous Quantitative Monitoring of Mural, Platelet-Dependent, Thrombus Kinetics in the Crushed Rat Femoral Vein

1991 ◽  
Vol 65 (04) ◽  
pp. 425-431 ◽  
Author(s):  
F Stockmans ◽  
H Deckmyn ◽  
J Gruwez ◽  
J Vermylen ◽  
R Acland
Keyword(s):  
Thrombus Formation ◽  
Femoral Vein ◽  
Maximum Size ◽  
Digital Analysis ◽  
Video Recordings ◽  
Quantitative Monitoring ◽  
Set Up ◽  
Kinetics Of ◽  
Quantitative Nature

SummaryA new in vivo method to study the size and dynamics of a growing mural thrombus was set up in the rat femoral vein. The method uses a standardized crush injury to induce a thrombus, and a newly developed transilluminator combined with digital analysis of video recordings. Thrombi in this model formed rapidly, reaching a maximum size 391 ± 35 sec following injury, after which they degraded with a half-life of 197 ± 31 sec. Histological examination indicated that the thrombi consisted mainly of platelets. The quantitative nature of the transillumination technique was demonstrated by simultaneous measurement of the incorporation of 111In labeled platelets into the thrombus. Thrombus formation, studied at 30 min interval in both femoral veins, showed satisfactory reproducibility overall and within a given animalWith this method we were able to induce a thrombus using a clinically relevant injury and to monitor continuously and reproducibly the kinetics of thrombus formation in a vessel of clinically and surgically relevant size

Growth and yield of okra (Abelmoschus esculentus (L.) Moench) grown in the marginal upland area of Sta. Rita, Samar as influenced by different planting densities and mulching materials

2017 ◽  
pp. 44-54
Author(s):  
Zenaida Gonzaga ◽  
Warren Obeda ◽  
Ana Linda Gorme ◽  
Jessie Rom ◽  
Oscar Abrantes ◽  
...  
Keyword(s):  
Bare Soil ◽  
Abelmoschus Esculentus ◽  
Growth And Yield ◽  
Planting Density ◽  
Rice Hull ◽  
Plastic Mulch ◽  
Lady’S Finger ◽  
Materials Used ◽  
Set Up ◽  
A Minor

Okra or Lady’s finger, botanically known as Abelmoschus esculentus (L.) Moench, is a tropical and sub-tropical indigenous vegetable crop commonly grown for its fibrous, slimy, and nutritious fruits and consumed by all classes of population. It has also several medicinal and economic values. Despite its many uses and potential value, its importance is under estimated, under-utilized, and considered a minor crop and little attention was paid to its improvement. The study was conducted to evaluate the effects of different planting densities and mulching materials on the growth and yield of okra grown in slightly sloping area in the marginal uplands in Sta. Rita, Samar, Philippines. A split-plot experiment was set up with planting density as main plot and the different mulching materials as the sub-plot which were: unmulched or bare soil, rice straw, rice hull, hagonoy and plastic mulch. Planting density did not significantly affect the growth and yield of okra. Regardless ofthe mulching materials used, mulched plants were taller and yielded higher compared to unmulched plants. Moreover, the use of plastic mulch resulted to the highest total fruit yield. The results indicate the potential of mulching in increasing yield and thus profitability of okra production under marginal upland conditions.

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

scholarly journals A System-Agnostic, Adaptable and Extensible Animal Support Cradle System for Cardio-Respiratory-Synchronised, and Other, Multi-Modal Imaging of Small Animals

Tomography ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 39-54
Author(s):  
Veerle Kersemans ◽  
Stuart Gilchrist ◽  
Philip Danny Allen ◽  
Sheena Wallington ◽  
Paul Kinchesh ◽  
...  
Keyword(s):  
Scientific Data ◽  
Cardiac Monitoring ◽  
Small Animals ◽  
Maintenance System ◽  
Wide Range ◽  
Set Up ◽  
Animal Handling ◽  
Imaging Performance ◽  
Significant Effort

Standardisation of animal handling procedures for a wide range of preclinical imaging scanners will improve imaging performance and reproducibility of scientific data. Whilst there has been significant effort in defining how well scanners should operate and how in vivo experimentation should be practised, there is little detail on how to achieve optimal scanner performance with best practices in animal welfare. Here, we describe a system-agnostic, adaptable and extensible animal support cradle system for cardio-respiratory-synchronised, and other, multi-modal imaging of small animals. The animal support cradle can be adapted on a per application basis and features integrated tubing for anaesthetic and tracer delivery, an electrically driven rectal temperature maintenance system and respiratory and cardiac monitoring. Through a combination of careful material and device selection, we have described an approach that allows animals to be transferred whilst under general anaesthesia between any of the tomographic scanners we currently or have previously operated. The set-up is minimally invasive, cheap and easy to implement and for multi-modal, multi-vendor imaging of small animals.

scholarly journals Multiple screening approaches reveal HDAC6 as a novel regulator of glycolytic metabolism in triple-negative breast cancer

2021 ◽  
Vol 7 (3) ◽  
pp. eabc4897
Author(s):  
Catríona M. Dowling ◽  
Kate E. R. Hollinshead ◽  
Alessandra Di Grande ◽  
Justin Pritchard ◽  
Hua Zhang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Histone Deacetylase Inhibitors ◽  
Triple Negative ◽  
Mitochondrial Apoptosis ◽  
Glycolytic Metabolism ◽  
Set Up ◽  
Screening Approaches

Triple-negative breast cancer (TNBC) is a subtype of breast cancer without a targeted form of therapy. Unfortunately, up to 70% of patients with TNBC develop resistance to treatment. A known contributor to chemoresistance is dysfunctional mitochondrial apoptosis signaling. We set up a phenotypic small-molecule screen to reveal vulnerabilities in TNBC cells that were independent of mitochondrial apoptosis. Using a functional genetic approach, we identified that a “hit” compound, BAS-2, had a potentially similar mechanism of action to histone deacetylase inhibitors (HDAC). An in vitro HDAC inhibitor assay confirmed that the compound selectively inhibited HDAC6. Using state-of-the-art acetylome mass spectrometry, we identified glycolytic substrates of HDAC6 in TNBC cells. We confirmed that inhibition or knockout of HDAC6 reduced glycolytic metabolism both in vitro and in vivo. Through a series of unbiased screening approaches, we have identified a previously unidentified role for HDAC6 in regulating glycolytic metabolism.

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