scholarly journals Electrical conductivity test for evaluation of the pea seed vigor

2020 ◽  
Vol 1 (1) ◽  
pp. 89
Author(s):  
Edvan Costa da Silva ◽  
Wagner Menechini ◽  
Carolina Dos Santos Galvão ◽  
Luís Augusto Batista de Oliveira ◽  
Natália Cássia de Faria Ferreira ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Pisum Sativum ◽  
Low Cost ◽  
Seed Vigor ◽  
State University ◽  
Randomized Experimental Design ◽  
Physiological Quality ◽  
Pea Seeds ◽  
The Individual

The electrical conductivity test is routinely used to differentiate the physiological quality of seed lots, and has stood out for its ease of execution, low cost, speed, repeatability, and straightforward interpretation of results. This study aimed to establish a methodology for the electrical conductivity test in pea seeds (Pisum sativum L.) involving seed quantity and seed imbibition periods. The experiment was carried out at the Multidisciplinary Laboratory of the State University of Goiás, the campus of Ipameri Campus, in 2018. Pea seeds (Pisum sativum) stored in the seed laboratory of the same institution were used for this experiment. The completely randomized experimental design with four replications in a 4x6 factorial scheme was used. Four quantity of seeds (25, 50, 75, and 100 seeds) and six imbibition periods (4, 8, 12, 16, 20, and 24 h) at 25ºC were evaluated. The electrical conductivity test was performed with four replicates of 25, 50, 75, and 100 seeds each, weighed on a precision scale (0.001g) and placed in disposable plastic cups, with a capacity of 200 mL, containing 75 mL of distilled water. The cups were then kept in a BOD chamber, set at 25°C, and the measuring was performed after 4, 8, 12, 16, 20, and 24 h of imbibition with a conductivity meter and results were expressed in μS cm-1 g-1. There were no significant results for the interaction between the number of seeds and the imbibition time. Significant results were observed only for the individual effects of each factor. It is recommended to use 100 seeds of Maria pea, and 24 h for the electrical conductivity test.

scholarly journals Physiological quality of mechanically harvested chickpea seeds

2021 ◽  
Vol 29 ◽  
pp. 28-35
Author(s):  
Letícia Betânia Xavier Dias ◽  
Pedro Afonso de Melo Queiroz ◽  
Thaís Cardoso de Castro ◽  
Marco Antonio Moreira de Freitas ◽  
Érica Fernandes Leão-Araújo ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Significant Interaction ◽  
Seed Quality ◽  
Rotation Speed ◽  
Mechanical Damage ◽  
Seed Vigor ◽  
Rotor Rotation ◽  
Germination Speed ◽  
Physiological Quality

Our objective was to evaluate the occurrence of mechanical damage in chickpea seeds with the combination of two harvester speeds (2.5 and 3.5 km.h-1) and three adjustments for rotor rotation speeds (500, 700, and 850 rpm). Harvesting was carried out in a seed production field. Seeds were evaluated for purity, germination (G), first count (FC), germination speed index (GSI), hypochlorite, electrical conductivity, and tetrazolium tests. There was an effect of harvester speeds on seed physiological quality for the first count (FC), germination (G), germination speed index (GSI), especially when combining with high rotor rotation speed. In these cases, the speed of 2.5 km.h-1 resulted in lower values. This harvester speed also had worse results when combining with 850 rpm for purity and hypochlorite tests. The tetrazolium test was not efficient in identifying differences in seed quality. There was no significant interaction between harvester speeds and rotor rotation speeds for the conductivity test. Evaluating the harvester speed’s isolated effect (3.5 km.h-1), we identified problems in seed vigor due to the higher value of exudates in the electrical conductivity test. Low harvester speed (2.5 km.h-1) associated with high rotor rotation speeds (700 and 850 rpm) causes a reduction of the physical and physiological quality of seeds.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

scholarly journals Evaluation of the seedvigor of Tournefortia paniculata Cham. post-digestive tract of the ruby-crowned tanager Tachyphonus coronatus (Vieillot, 1822)

2019 ◽  
Vol 41 ◽  
pp. 15
Author(s):  
Sérgio Thode Filho ◽  
Heider Alves Franco ◽  
Emanuele Nunes de Lima Figueiredo Jorge ◽  
Mariana De Souza Oliveira ◽  
Jhon Lenon Genovez de Oliveira ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Digestive Tract ◽  
Digestive System ◽  
Forest Species ◽  
Ecological Process ◽  
Germination Test ◽  
Individual Level ◽  
Physiological Quality ◽  
The Individual

Endozoochory (dispersion with passage through the digestive system of animals) is one of the most important processes. It is included in the ecological process known as dispersive mutualism, benefit the species involved. The most used method to evaluate the physiological quality of seeds is carried out based on the traditional germination test. However, electrical conductivity tests with seeds of forest species are still scarce, especially those developed with the individual method, which provide greater fidelity to the results so that through this one can define the test by the mass methods. The current work aimed to investigate the vigor, from the electrical conductivity of the seeds of Tournefortia paniculata Cham. after passage through the digestive tract of a ruby-crowned tanager Tachyphonus coronatus (Vieillot, 1822). An expedition was conducted in a fragmented area of Atlantic Forest in process of reforestation in the campus of the IFRJ. After the capture of the passerine with mist nets, proceeded the identification of seeds shed in its feces. The experimental design was completely randomized with one treatments with five replicates. The results allowed concluded that the seed of the T. paniculata Cham. after digestive tract of the ruby-crowned tanager presented high electrical conductivity in three of its replicates at the individual level. 

scholarly journals Influence of physiological and health quality on the vigor of cucumber seeds

2019 ◽  
Vol 86 ◽  
Author(s):  
Vanessa Nogueira Soares ◽  
Francisco Amaral Villela ◽  
Aline Klug Radke ◽  
Hélen Claudine Saliba Rodrigues ◽  
Vanessa Pinto Gonçalves ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Seedling Emergence ◽  
Seed Vigor ◽  
Physiological Performance ◽  
Penicillium Sp ◽  
Cucumber Seed ◽  
Health Quality ◽  
Physiological Quality ◽  
Controlled Deterioration

ABSTRACT The physiological performance of seeds is related to their physiological quality and seed vigor, while their health quality may interfere with germination and early seedling establishment in the field due to the interaction of microorganisms associated with seeds. The study aimed to evaluate the physiological performance and health quality of cucumber seeds and to verify the relationship between these attributes. The physiological quality of cucumber seeds was evaluated by standard germination tests, first count of germination, controlled deterioration test, electrical conductivity test, seedling emergence, emergence speed index, and traditional accelerated aging and aging modified with saline for 48, 72, and 96 h. The health quality of cucumber seed lots was evaluated by blotter test. Stratification of cucumber seed lots by seedling emergence was similar to seedling emergence by controlled deterioration test, first count of germination, and electrical conductivity results. The cucumber seed lots evaluated showed high germination rates; however, lots 1 and 3 had a better performance in vigor tests than lots 2 and 4. Fungi detected in the blotter test were Alternaria sp., Aspergillus sp., Cladosporium sp., and Penicillium sp. Seed lots 2 and 4 had low vigor evaluated by seedling emergence and controlled deterioration, and showed a higher incidence of Penicillium sp. in the evaluation of health quality of seeds. The incidence of Penicillium sp. may negatively affect the vigor of cucumber seeds evaluated by seedling emergence and by controlled deterioration test.

scholarly journals Pre-packing cooling and types of packages in maintaining physiological quality of soybean seeds during storage

2016 ◽  
Vol 38 (2) ◽  
pp. 129-139 ◽  
Author(s):  
Everson Reis Carvalho ◽  
João Almir Oliveira ◽  
Denilson Paulo da Rosa Mavaieie ◽  
Hellismar Wakson da Silva ◽  
Cassiano Gabriel Moreira Lopes
Keyword(s):  
Electrical Conductivity ◽  
Experimental Design ◽  
Seed Mass ◽  
Accelerated Aging ◽  
Soybean Seeds ◽  
Randomized Experimental Design ◽  
Deterioration Process ◽  
Physiological Quality ◽  
Polyethylene Container

Abstract: Seeds storing under appropriate conditions reduces the speed of the seeds deterioration process. The aim of this study was to evaluate pre-packing cooling and types of packages in the maintenance of the physiological quality of soybean seeds during storage. The completely randomized experimental design was used, with four replications, considering a factorial arrangement involving or not the pre-packing cooling of the seed mass, three types of packages (multi-layer paper bag, big bag, and polyethylene container), and five storing periods (0, 2, 4, 6 and 8 months). The physiological quality was evaluated by the tests of first germination count, germination, accelerated aging, electrical conductivity, emergence speed index and emergence. The use of packages with larger amount of seeds (big bag and polyethylene container) did not favor the maintenance of the pre-packing cooling temperature. The types of packages did not show significant differences in the seeds viability during storage, up to eight months, regardless the pre-packing cooling. The cultivars seeds showed different tolerance to storing, i.e. seeds of the cultivar TMG 1176 had lower storing potential comparing to the seeds from the cultivar SYN 9074.

scholarly journals ELECTRICAL CONDUCTIVITY IN Piptadenia moniliformis Benth. SEED LOTS CLASSIFIED BY SIZE AND COLOR

2016 ◽  
Vol 40 (5) ◽  
pp. 855-866
Author(s):  
Gilvaneide Alves Azeredo ◽  
Rinaldo César de Paula ◽  
Sérgio Valiengo Valeri
Keyword(s):  
Electrical Conductivity ◽  
Sulfuric Acid ◽  
Seed Vigor ◽  
Round Hole ◽  
Seed Retention ◽  
Physiological Quality ◽  
Concentrated Sulfuric Acid ◽  
Number Of Seeds

ABSTRACT Among the most widely used tests for evaluating seed vigor, electrical conductivity (EC) stands out as one of the most promising due to its speed and simplicity. This study aimed to verify if the EC test could be used to evaluate the physiological quality of seed lots of Piptadenia moniliformis Benth. A seed lot, which was classified by seed tegument color and size, was used based on seed retention in round-hole screens, sizes 11 and 15, constituting 10 sub-lots. Prior to germination and the EC test, the seeds were immersed in concentrated sulfuric acid for 20 minutes in order to overcome tegument dormancy. During the EC test, we soaked seeds in 75 mL of water at 25 ºC and tested eight soaking periods (2, 4, 6, 24, 48, 72, 96, and 120 h) and two seed quantities (25 and 50) per replicate. The EC test, regardless of the number of seeds or soaking period, was not adequate for evaluating the physiological quality of color- or size-classified seed lots from P. moniliformis Benth. .

scholarly journals Desempenho fisiológico de sementes de algodão cultivadas em Luís Eduardo Magalhães, Bahia

2015 ◽  
Vol 10 (2) ◽  
pp. 69
Author(s):  
R. T. C. Nunes ◽  
T. R. Prado ◽  
E. B. Ribeiro ◽  
W. S. Vale ◽  
O. M. Morais
Keyword(s):  
Electrical Conductivity ◽  
Gossypium Hirsutum ◽  
Seed Quality ◽  
Germination Rate ◽  
Dry Mass ◽  
State University ◽  
Seed Technology ◽  
Physiological Quality ◽  
Cotton Seeds

<p>O presente trabalho foi desenvolvido no laboratório de tecnologia de sementes da Universidade Estadual do Sudoeste da Bahia, Campus de Vitória da Conquista UESB, com objetivo de avaliar a qualidade fisiológica de sementes de algodão (<em>Gossypium hirsutum</em> L.), utilizando-se cinco cultivares (DP 604, FM 993, BRS 368, TMG 642 e DELTA OPAL). As sementes foram submetidas aos seguintes testes: teor de água, peso de mil sementes, germinação, primeira contagem de germinação, índice de velocidade de emergência, emergência, comprimento da parte aérea, massa seca das plântulas e condutividade elétrica. O delineamento experimental utilizado foi o inteiramente casualizado, em quatro repetições de 50 sementes por tratamento. A cultivar TMG 642 demonstrou baixa qualidade fisiológica das sementes, quando comparados com as cultivares DP 604, FM 993, BRS 368, e DELTA OPAL. Os testes de germinação, condutividade elétrica e índice de velocidade de germinação mostraram eficiência na separação de cultivares de sementes de algodão em níveis de vigor. </p><p align="center"><strong><em>Physiological performance of cottonseed grown in Luís Eduardo Magalhães, Bahia</em></strong></p><p><strong>ABSTRACT</strong><strong>: </strong>This study was conducted at the State University of seed technology laboratory of Southwest Bahia, Campus Victory Conquest, UESB, to evaluate the physiological quality of cotton seeds (<em>Gossypium hirsutum</em> L.), using five cultivars (DP 604, FM 993, BRS 368, GMT 642 and DELTA OPAL). Seeds were subjected to the following tests: water content, weight of a thousand seeds, germination, first count, emergence speed index, emergency, shoot length, dry mass of seedlings and electrical conductivity. The experimental design was completely randomized, with four replications of 50 seeds per treatment. The cultivar TMG 642 demonstrated low physiological seed quality when compared with the DP 604 cultivars, FM 993, BRS 368, and DELTA OPAL. Germination tests, electrical conductivity and germination rate index showed efficiency in the separation of cotton seed varieties in force levels.</p>

scholarly journals Massal and Individual Electrical Conductivity Tests and its Relationships with the Physiological Quality of Sweet Sorghum Seeds

2020 ◽  
Vol 8 (2) ◽  
pp. 407
Author(s):  
Joner Silveira Dalcin ◽  
Caren Alessandra Müller ◽  
Ubirajara Russi Nunes ◽  
Rodrigo Roso ◽  
Alberto Cargnelutti Filho ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Cold Test ◽  
Dry Mass ◽  
Shoot Length ◽  
Tukey Test ◽  
Physiological Quality ◽  
Rapid Tests ◽  
The Individual ◽  
The Relationship

Rapid tests such as electrical conductivity provide fast and consistent results regarding stratification of seed lots by vigor. They are important tools used to evaluate the physiological quality of seeds of several species, however, no such information was found for Sorghum bicolor. The objective of this study was to evaluate the relationship between the physiological quality of the seeds by the mass and individual conductivity tests. Four plots of the Fepagro 19 cultivar were analyzed through the degree of moisture, weight of one thousand seeds, first count and germination, radicular and shoot length, dry mass, cold test and emergence of field seedlings. Subsequently, they were submitted to mass electrical conductivity experiments with variations in the imbibition time (2, 4, 6, 8, 12 and 24 hours), temperature (20, 25 and 30 ºC), number of seeds (25 and 50), volume of water (50 and 75 mL) and individual (2, 4, 6, 8, 12 and 24 hours). The design was completely randomized and the data evaluated by the Tukey test. Only the lowest physiological quality batch was detected in the 20 °C, 75 mL and 25 seeds for 12 hours combinations; the individual test detected the least physiological quality batch, and it was possible to perform it in 24 hours.

scholarly journals Prior disinfestation of irrigated rice seeds under laboratory conditions favors expression of physiological potential

2020 ◽  
Vol 19 (4) ◽  
pp. 494-497
Author(s):  
Bruno Tabarelli Scheidt ◽  
Jaquelini Garcia ◽  
Ricardo Trezzi Casa ◽  
Cileide Maria Medeiros Coelho ◽  
Juliano Berghetti
Keyword(s):  
Experimental Design ◽  
State University ◽  
Germination Test ◽  
Irrigated Rice ◽  
Seed Analysis ◽  
Randomized Experimental Design ◽  
Physiological Quality ◽  
Analysis Laboratory ◽  
Rice Seeds

The purpose of this study was to analyze prior seed disinfestation of irrigated rice seeds with sodium hypochlorite and its effect on physiological quality. The experiment was conducted in the seed analysis laboratory of Santa Catarina State University (UDESC). Twenty-four (four per cultivar) seed lots of the cultivars SCSBRS Tio Taka, Epagri 109, SCS116 Satoru, SCS118 Marquês, SCS121 CL, and SCS122 Miura, produced in the Upper Itajaí Valley in the 2016/2017 season, were used in a completely randomized experimental design with four replications. Germination and vigor tests were performed with and without disinfestation seeds. For the germination test with disinfected seeds, there was a higher percentage of normal seedlings and a smaller number of abnormal seedlings and dead seeds. There was significant decrease in vigor without prior seed disinfestation. The fungi that infested the seeds caused an increase in the percentage of abnormal seedlings and dead seeds. Seed disinfestation prior to physiological test positively affects the results regarding the physiological quality of irrigated rice seeds.

scholarly journals Electrical Conductivity Test to Evaluate the Physiological Quality of Salvia hispanica L. Seeds

2018 ◽  
Vol 10 (3) ◽  
pp. 377
Author(s):  
A. K. Radke ◽  
D. W. Maass ◽  
M. S. Pieper ◽  
F. M. Xavier ◽  
A. B. N. Martins ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Seed Vigor ◽  
Herbaceous Plant ◽  
Salvia Hispanica ◽  
Control Programs ◽  
Chia Seed ◽  
Physiological Quality ◽  
Salvia Hispanica L ◽  
Different Levels

Chia (Salvia hispanica L.) is an herbaceous plant that belongs to the Lamiaceae family. Seed vigor testing is an important component of quality control programs, and electrical conductivity test is a possible option in this process. The objective of this study was to identify a methodology of electrical conductivity test to stratify chia seed lots at vigor level according to the emergence of seedlings. Six lots of chia seeds were used in this study. The seeds were exposed to the following determinations: first germination count, germination, emergence speed index, emergence, aerial and root length, aerial and root dry matter, and electrical conductivity at 25, 50 and 75 mL during 1, 2, 4, 6, 8 and 24 hours of soaking in water. The electrical conductivity test in the combination of 25 seeds using 50 mL of water in 6-hour of soaking period, as well as the combination of 50 seeds using 50 mL of water for 24 hours of soaking, were efficient in the stratification of chia seeds in different levels of vigor.

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