scholarly journals Role of Emblica officinalis (amla) in the prophylaxis of hepatic injury by carbon tetrachloride (CCl4) in albino rats

2017 ◽  
Vol 6 (8) ◽  
pp. 1992
Author(s):  
Chinmoyee Deori ◽  
Swarnamoni Das ◽  
Sushanta Kumar Bordoloi
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Injury ◽  
Cost Effective ◽  
Hepatoprotective Activity ◽  
Modern Medicine ◽  
Albino Rats ◽  
Emblica Officinalis ◽  
Oral Toxicity ◽  
Standard Drug ◽  
Percolation Method

Background: The objective of the study was to evaluate the hepatoprotective activity of fruits extract of Emblica officinalis on carbon tetrachloride induced hepatotoxicity in Albino rats. Liver is vulnerable to wide variety of metabolic, toxic, microbial, circulatory and neoplastic insults. The dominant primary diseases of the liver are viral hepatitis, alcoholic liver disease and hepatocellular carcinoma. Due to absence of reliable drugs management of liver ailments in the modern medicine, plants and natural products are providing to be good hepatoprotectants as evident from voluminous published work on their hepatoprotective potentials.Methods: The required amount of fresh fruits of Emblica officinalis was collected and dried at room temperature. The dried fruit ground into powdered and was prepared by percolation method using 95% ethanol. Acute oral toxicity test of the fruits extract was performed as per OECD 423 guidelines. Carbon tetrachloride was used as the hepatotoxin. Silymarin suspension 100mg was used as a standard drug in this study.Results: The study was carried out with an attempt to evaluate the hepatoprotective activity of Emblica officinalis in carbon tetrachloride induced hepatic injury in albino rats. The results were analysed by using one way ANOVA followed by Dunnett’s test and (p <0.05) was considered significant.Conclusions: The present study shows that the Emblica officinalis has significant hepatoprotective activity in rats with carbon tetrachloride induced liver injury. Emblica officinalis is a commonly used traditional herb, which is safe, cost effective and grows widely in India and used by different ethnic groups for various diseases. That is why Emblica officinalis, a commonly used natural herb, deserve further consideration in order to establish its potential as safe economical hepatoprotective agent.

scholarly journals HEPATOPROTECTIVE EFFECTS OF POLYHERBAL FORMULATION AGAINST CARBON TETRACHLORIDE-INDUCED HEPATIC INJURY IN ALBINO RATS: A TOXICITY SCREENING APPROACH

2016 ◽  
Vol 10 (1) ◽  
pp. 192 ◽  
Author(s):  
Bhumika Yogi ◽  
Ashutosh Mishra
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Injury ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Calotropis Procera ◽  
Albino Rats ◽  
Gymnema Sylvestre ◽  
Toxicity Screening ◽  
Lawsonia Inermis ◽  
Polyherbal Formulation

ABSTRACTObjective: To investigate the hepatoprotective activity of aqueous methanolic extract of polyherbal formulation and individual extracts of Calotropisprocera, Gymnema sylvestre, and Lawsonia inermis leaves.Methods: The hepatoprotective activity of the formulation and extracts were assessed on the basis of histopathological changes in liver of carbontetrachloride-induced hepatotoxic rats.Results: The result of present studies strongly indicates that the hepatoprotective property of polyherbal formulation as evidenced by less damagedhepatocytes cells.Conclusion: Polyherbal formulation revealed excellent hepatoprotective profile as compared to individual plants due to synergistic effect producedby different phytochemicals present in the formulation.Keywords: Calotropis procera, Gymnema sylvestre, Lawsonia inermis, Carbon tetrachloride.

Assessment of hepatoprotective activity of coded plant (222) leaf ethanolic extract against carbon tetrachloride-induced hepatotoxicity in Wistar rats – Part IV

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Krishnakumar N M
Keyword(s):  
Carbon Tetrachloride ◽  
Biochemical Parameters ◽  
Subcutaneous Administration ◽  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Albino Rats ◽  
Hepatic Enzymes ◽  
Standard Drug

The present study was designed to assess the possible hepatoprotective activity of the leaf ethanolic extract of coded plant (Code No. 222**) against carbon tetrachloride (CCl4)-induced hepatic injury in Wistar albino rats. The animals were divided into different groups and treated with 222 leaf ethanolic extract at different concentrations for five days. Silymarin, the known hepatoprotective standard compound (100 mg/kg) was administered for five days. Hepatotoxicity was induced by the subcutaneous administration of a single dose of CCl4: Olive oil (2 mL/kg) on days 2 and 3. The administration of CCl4 resulted in marked increase in serum hepatic enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and serum bilirubin levels. CCl4 intoxication also resulted in a significant (P=0.05) increase in malondialdehyde (MDA), which is a common marker of lipid peroxidation. The other biochemical parameters such as cholesterol, triglycerides, creatinine, urea and uric acid levels were also increased significantly (P=0.05) compared to normal control group. Changes in serum hepatic enzymes, biochemical parameters and MDA levels induced by CCl4 were reversed by the leaf ethanolic extract of 222 (125 mg/kg) dose. The standard drug silymarin treated group also reversed CCl4-induced changes in biomarkers of liver function and MDA levels. Histopathological studies of the liver samples confirmed the hepatoprotective property of the coded drug 222. It was seen that histopathological damage induced by CCl4 were improved in rat liver, treated with 222 extract. The results of the present study suggested that coded plant (222) leaf ethanolic extract may be used as a hepatoprotective agent against toxic effects caused carbon tetrachloride in the liver.

scholarly journals MULTIPLE TREATMENT OF EREMURUS HIMALAICUS EXTRACTS AMELIORATES CARBON TETRACHLORIDE INDUCED LIVER INJURY IN RATS

2016 ◽  
Vol 8 (9) ◽  
pp. 24 ◽  
Author(s):  
Ahlam Mushtaq ◽  
Mubashir Hussain Masoodi ◽  
Adil Farooq Wali ◽  
Bashir Ahmad Ganai
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Western Himalayas ◽  
Tissue Samples ◽  
Standard Drug ◽  
Multiple Treatment ◽  
Wistar Strain ◽  
Serum Biochemical ◽  
North Western

<p><strong>Objective: </strong><em>Eremurus himalaicus </em>Baker<em>, </em>an edible herb of North Western Himalayas, has not been scientifically assessed for hepatoprotective potential. The ethyl acetate extract (EHE), methanolic extract (EHM) and aqueous extract (EHA) of <em>Eremurus himalaicus </em>were therefore evaluated for potential hepatoprotective activity in Wistar strain albino rats.</p><p><strong>Methods: </strong>Carbon tetrachloride (1.5 ml/kg) was employed as hepatotoxin and was given on Day 1 of the experiment. The extracts at a dose of 300 mg/kg bw (EHE, EHM and EHA) and the standard at a dose of 10 mg/kg bw (Liv 52) were given for following 7 d and the biochemical parameters (SGOT, SGPT, ALP, TP, bilirubin and UA) were estimated in order to assess the liver function. Moreover, the liver tissue samples were examined for histopathological changes.</p><p><strong>Results: </strong>The results for serum biochemical analysis in rats showed a rise in SGOT, SGPT, ALP and bilirubin levels and a decrease in TP and UA levels upon giving hepatotoxin. The administration of the extracts and standard drug, for a period of 7 d, showed a significant decrease in SGOT, SGPT, ALP and bilirubin levels and an increase in TP and UA levels for EHM when compared to the toxic group. These results correlated well with the histopathological findings of liver for normal, toxic and extract treated groups. The EHM treatment decreased the extent of fat deposition and necrosis caused by CCl<sub>4</sub>. The results were almost similar to the standard drug Liv 52.</p><p><strong>Conclusion: </strong>Collectively; the results indicate that EHM exhibits significant hepatoprotective activity against CCl<sub>4 </sub>induced hepatotoxicity.</p>

scholarly journals EVALUATION OF HEPATOPROTECTIVE POTENTIALS OF METHANOL EXTRACT OF TEPHROSIA VILLOSA AGAINST THIOACETAMIDE INDUCED LIVER TOXICITY IN ALBINO RATS

2021 ◽  
pp. 76-81
Author(s):  
PINKEY RAWAL ◽  
RAMESH C ◽  
SOMA PRAMANIK ◽  
SHABANA S
Keyword(s):  
Methanol Extract ◽  
Liver Toxicity ◽  
Total Duration ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Clotting Time ◽  
Oral Toxicity ◽  
Dose Administration ◽  
Standard Drug ◽  
Protein Ions

Objective: The present study was conducted to determine the hepatoprotective potentials of methanol s extracts of Tephrosia villosa leaves against thioacetamide (TAA) induced liver damage in rats. Methodology: The acute oral toxicity study was conducted as per OECD guidelines, and the extract was proved to be safe up to the dose of 2000 mg/kg. The total duration of the study was 21 days, and animals were divided into six groups. Hepatotoxicity was induced in the animals of all groups except normal control by single dose administration of TAA (100 mg/kg) at 1st day of the study followed by animals were treated daily with standard drug silymarin and methanol extract of T. villosa (100 mg/kg, 200 mg/kg and 400 mg/kg) to respective groups for 21 days. Variations in biochemical parameters such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, direct bilirubin, albumin, total protein, ions and others parameters such as clotting time and weight of the liver were considered to determine beneficial effect of the extract. At the end of the study liver samples were collected and subjected to histopathological evaluation. Results: In control animals treated with TAA alone, there were variations in the above mentioned parameters. However in the animals treated with methanol extract and standard drug silymarin, all the parameters were normal possibly due to their beneficial property in protecting the liver against TAA induced hepatotoxicity. Conclusion: The results obtained in the above study suggesting that, the methanol extract of T. villosa possess significant hepatoprotective activity.

scholarly journals Protective Effect of Ethanolic Extract ofTabernaemontana divaricata(L.) R. Br. against DEN and Fe NTA Induced Liver Necrosis in Wistar Albino Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Kannappan Poornima ◽  
Palanisamy Chella Perumal ◽  
Velliyur Kanniappan Gopalakrishnan
Keyword(s):  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatic Necrosis ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Protective Effects ◽  
Liver Necrosis ◽  
Standard Drug ◽  
Tabernaemontana Divaricata ◽  
Wistar Albino Rats

This study is an attempt to evaluate the hepatoprotective activity ofTabernaemontana divaricataagainst DEN and Fe NTA induced liver necrosis in rats. Ethanolic extract of the whole plant ofTabernaemontana divaricataat doses of 200 and 400 mg/kg body weight and 5-fluorouracil (standard drug) was orally administered to male Wistar Albino rats once daily for 24 weeks, simultaneously treated with the carcinogen DEN and Fe NTA. In simultaneously treated animals, the plant extract significantly decreased the levels of uric acid, bilirubin, AST, ALT, and ALP in serum and increased the levels of liver marker enzymes in liver. Treatment with the extracts resulted in a significant increase in the levels of antioxidants accompanied by a marked reduction in the levels of malondialdehyde when compared to DEN and Fe NTA treated group. When compared with 200 mg/kg bw rats, 400 mg/kg bw rats and 5-fluorouracil treated rats showed better results in all the parameters. The histopathological studies confirmed the protective effects of extract against DEN and Fe NTA induced liver necrosis. Thus, it could be concluded that the use ofTabernaemontana divaricataextract in the treatment of carcinogen induced hepatic necrosis.

scholarly journals Evaluation of Hepatoprotective and in vivo Antioxidant Potentials of Methanol Extract of Tephrosia pumila against Thioacetamide Induced Liver Toxicity in Wistar Rats

2021 ◽  
Vol 70 (2) ◽  
Author(s):  
Ramesh C ◽  
Pinkey Rawal ◽  
Soma Pramanik ◽  
Shabana S
Keyword(s):  
Biochemical Parameters ◽  
Methanol Extract ◽  
Liver Toxicity ◽  
Antioxidant Properties ◽  
Total Duration ◽  
Hepatoprotective Activity ◽  
Oral Toxicity ◽  
Standard Drug ◽  
Protein Ions

The objective of the current investigation was performed to assess the hepatoprotective potentials and in vivo antioxidant properties of methanol extract of Tephrosia pumila against thioacetamide induced liver damage in rats. The acute oral toxicity study of methanol extract was determined as per OECD guidelines and the extract was proved to be safe up to the dose of 2000mg/kg. The total duration of the study was 21 days and animals were divided into six groups. Hepatotoxicity was induced in the animals of all groups except normal control by single dose administration of Thioacetamide(100mg/kg) at first day of the study followed by animals were treated daily with standard drug sylimarin and methanol extract of Tephrosia pumila (100mg/kg, 200mg/kg and 400mg/kg) to respective groups for 21 days. Variations in biochemical parameters like alanine transferase (ALT), aspartate transferase (AST), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, albumin, total protein, ions and others parameters like clotting time and weight of the liver were considered to determine beneficial effect of the extract. At the end of the study liver samples were collected and subjected to histopathological evaluation. There were significant variations in the above mentioned biochemical parameters in toxic control animals treated with Thioacetamide alone while in the animals treated with methanol extract and standard drug silymarin, all the parameters were normal possibly due to their beneficial property in protecting the liver against thioacetamide induced hepatotoxicity. The results obtained in the above study suggesting that, the methanol extract of Tephrosia pumila possess significant hepatoprotective activity.

scholarly journals Evaluation of hepatoprotective potential of aqueous extract of Withania somnifera in albino rats

2021 ◽  
Vol 10 (3) ◽  
pp. 255
Author(s):  
Monica Sharma ◽  
Anand Gaur ◽  
Pinki Vishwakarma ◽  
Raj Kumar Goel ◽  
K. K. Saxena
Keyword(s):  
Aqueous Extract ◽  
Withania Somnifera ◽  
Work Force ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Standard Drug ◽  
Hepatic Diseases ◽  
Organ Systems ◽  
Herbal Plant ◽  
Biochemical Examination

Background: Hepatic diseases are a major cause of morbidity and disability of work force throughout the world. The treatment of hepatic diseases with standard drugs poses the risk of toxicity on various organ systems. Withania somnifera, a herbal plant has been claimed to be effective in the treatment of various types of hepatic conditions. The present study was undertaken to explore the hepatoprotective activity of aqueous extract of Withania somnifera (AEWS) in experimentally induced hepatotoxicity in albino rats.Methods: The study was commenced after obtaining approval from institutional animal ethical committee using AEWS leaves in Albino wistar rats (150-200 gm) of either sex. The hepatoprotective activity was evaluated using biochemical examination. The animals were divided into five groups of six animals each. In each experiment, first group was given normal saline (1 ml/kg/day), second group was injected with toxin CCl4 (1 ml/kg) i.p only once to produce hepatotoxicity, third and fourth groups were given Withania somnifera orally (500 mg/kg and 1000 mg/kg) (respectively), as a single dose per orally every morning and fifthgroup was given standard drug Liv-52 (1 mg/kg).Results: Aqueous extract of Withania somnifera leaves in oral dose exhibited significant hepatoprotective effect in all models used in this study.Conclusions: It can be concluded from our study that aqueous extract of Withania somnifera leaves possesses hepatoprotective activity.

Evaluation of Hepatoprotective activity of Methanol extract of Persicaria hydropiper: An in vivo screening

2021 ◽  
pp. 5819-5824
Author(s):  
Pooja Kamra ◽  
Mahaveer Singh ◽  
Hardarshan Singh Lamba ◽  
Birendra Srivastava
Keyword(s):  
Carbon Tetrachloride ◽  
Intraperitoneal Administration ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Standard Drug ◽  
Whole Plant ◽  
Dose Dependent ◽  
Histopathological Investigation ◽  
Different Doses

The present study aimed to evaluate the hepatoprotective potential of methanolic whole plant extract of Persicaria hydropiper in carbon tetrachloride (CCl4) induced hepatotoxicity model. Hepatotoxicity was induced in rats by intraperitoneal administration of carbon tetrachloride (CCl4) for seven days. The extract was thereafter administered at two different doses of 200 mg/kg and 400 mg/kg body weight for next seven days. Silymarin was used as a reference standard. The extract revealed hepatoprotective activity in dose dependent manner. The dose of 400 mg/kg exhibited maximum hepatoprotective ability as apparent from several evaluation parameters including liver function profile, bilirubin, antioxidant enzymes as well as histopathological investigation which was comparable to the standard drug Silymarin respectively. These findings sustenance the use of the extract as an adjuvant with existing therapy for treatment of liver ailments.

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