scholarly journals Study of expression of PTEN and Cyclin D1 in endometrium at a tertiary care centre

2019 ◽  
Vol 6 (2) ◽  
pp. 495
Author(s):  
Shaffy Thukral ◽  
Shuaeb Bhat ◽  
Nusrat Bashir
Keyword(s):  
Cyclin D1 ◽  
Tertiary Care ◽  
Inverse Correlation ◽  
Premalignant Lesions ◽  
P Value ◽  
Nuclear Staining ◽  
Tertiary Care Centre ◽  
Chi Square ◽  
Normal Endometrium ◽  
Chi Square Test

Background: To study the expression of PTEN (Phosphatase and Tensin homologue) and Cyclin D1 in normal, hyperplastic and neoplastic endometrium by immunohistochemistry and to corroborate the interrelationship between PTEN and Cyclin D1 in normal to neoplastic endometrial disorders including endometrial carcinoma.Methods: Formalin fixed paraffin embedded (FFPE) sections of spectrum of endometrium in fifty different cases were taken from secretory phase to endometrial carcinoma and subjected to Immunohistochemistry using PTEN and Cyclin D1 .Results: Immunoreactivity was regarded as positive when brown staining was localized in the nuclei or cytoplasm. The intensity of nuclear staining was graded from 0 to 3+ and the extent was semi quantitatively estimated. If less than 10% of cells were positive a score of 0 was given, 11 % to 30% cell positivity was scored as 1+, 31% to 60 % positivity was scored as 2+ and more than 60% positive cells was labelled as 3+. Statistical analysis was performed with Chi-Square test and significant differences were noted between these 3 groups (p value < 0.05).Conclusions: The present study supports that an inverse correlation exists in the expression of PTEN and Cyclin D1 in normal, hyperplastic and neoplastic endometrium. The decreased PTEN expression is a marker of the earliest endometrial premalignant lesions, and we propose that use of PTEN immunostaining may be informative in identifying premalignant lesions that are likely to progress to carcinoma. Cyclin D1 expression in endometrial glands increases progressively in intensity and extent from normal endometrium to hyperplasia to carcinoma.

Comparison of Clinical Outcomes With Drug Coated Balloons Versus Plain Balloon Angioplasty In Chronic Limb-Threatening Ischemia

2021 ◽  
pp. 153857442110225
Author(s):  
Joel Mathew John ◽  
Vimalin Samuel ◽  
Dheepak Selvaraj ◽  
Prabhu Premkumar ◽  
Albert A Kota ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Interventional Procedure ◽  
Tertiary Care ◽  
Arterial Disease ◽  
Multivariable Logistic Regression Analysis ◽  
Tertiary Care Centre ◽  
Chi Square ◽  
Exact Test ◽  
Institutional Review ◽  
Chi Square Test

Objective: The use of drug coated balloon (DCB) for angioplasty has shown superior efficacy against plain balloons for treating complex infrainguinal arterial disease. We report and compare the clinical outcomes following application of DCB(Paclitaxel) and plain angioplasty (POBA) in our tertiary care centre. Methods: A retrospective, single centre analysis of 301 patients with chronic limb-threatening ischemia involving the infrainguinal segment was conducted between September 2014 and September 2018, after approval from the Institutional review board. We analyzed clinical outcomes by measuring postoperative ABI improvement, restenosis requiring reintervention procedure, minor and major amputations at the end of 18 months. . To find the association between the group variables (POBA and DCB) and other risk variables, Chi-square test/Fisher’s exact test was used. Multivariable logistic regression analysis was used. Results: Patients who underwent treatment with plain balloon (POBA) and DCB(Paclitaxel) angioplasty were 246(81.7%) and 55(18.3%) respectively. Our study group was predominantly male (Male: Female = 6.7:1), most patients were more than 50 years of age (n = 251, 83.4%). Smoking (n = 199, 66.1%) and diabetes (n = 210, 69.8%) were the most common atherosclerotic risk factors. Postoperative Ankle Brachial Pressure Index (ABI) improvement were similar in both groups (POBA = 57.7%; DCB = 69.8%; p = 0.103). Minor and major amputations following POBA were 26% and 22%; and DCB were 12.7% and 16.4% respectively. Re-stenosis requiring a re-interventional procedure within 18 months was 15%, (n = 37) following POBA; and 12.7% (n = 7) following DCB (p = 0.661). Conclusions: This retrospective study shows similar clinical limb related outcomes following POBA and DCB at 18 months. However, our comparative analysis between the POBA and DCB groups was totally unadjusted and not adjusted for common confounders such as age and sex. Hence, for one to draw definitive conclusions leading to changes in clinical practice; a randomized, prospective study with a larger patient cohort is needed.

scholarly journals 219. Is the modified quick SOFA scale superior to quick SOFA in patients with diagnosed septic shock?

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S128-S128
Author(s):  
Deniz Akyol ◽  
Ayşe Uyan Önal ◽  
Uğur Önal ◽  
Damla Akdağ ◽  
Cansu Bulut Avşar ◽  
...  
Keyword(s):  
Septic Shock ◽  
Survival Rate ◽  
Tertiary Care ◽  
University Hospital ◽  
P Value ◽  
Chi Square ◽  
Number Of Patients ◽  
Significant Difference ◽  
Chi Square Test ◽  
Qsofa Score

Abstract Background In this study it was aimed to compare the effects of qSOFA (Quick Sequential Organ Failure Assessment) score with modified qSOFA score (PLoS One. 2018 Sep 26;13(9):e0204608) for predicting one month survival in patients with diagnosed septic shock (SS) in a tertiary-care educational university hospital in a developing country. Methods Modified qSOFA was created by adding age factor (>50 years=1 point) to patients with qSOFA scale 1 or 2 or 3 who had SS (sepsis+hypotension+adrenergic agent) and consulted by Infectious Diseases consultants between December 2013-December 2018. Arterial lactate level of >2 mmol/L criterion was added as an including criteria for SS according to 3rd International Sepsis and Septic Shock Consensus Statement after 23rd February 2016. Statistical analysis was performed via Chi-square test and a p-value <0.05 was considered significant. Results The number of patients with qSOFA score of 1 or 2 or 3 from 527 patients are in Table1 [some of the cases were diagnosed as septic shock according to elder definition (without lactate criterion) and there was a subgroup with qSOFA score 1]. Among the >50-year aged group, the 30-day survival rate was lower in patients with qSOFA3 vs. qSOFA 2 vs. qSOFA 1 (Table1, 3x2 Chi Square test, P = 0.0057). Among the <50 years group, the qSOFA one month survival rate was lower in patients with qSOFA 3 vs. qSOFA 2 vs. qSOFA 1 (Table, 3x2 Chi Square Test, P = 0.0052). According to modified qSOFA, there was a significant difference for one month survival among SS cases with scores of 1, 2, 3 and 4 (12/21 57% vs. Fifty/126 40% vs. 78/269 29% vs. 22/111 20%, 4x2 Chi-square test, P = 0.0003). On the other hand, there was no significant difference in terms of one month survival when we performed subgroup analysis in qSOFA score 1, 2, or 3 subgroups, as ≤50 years vs. >50 years (table, Chi-square test, 12/21 vs. 39/97 P = 0.224, 11/29 vs. 75/244 P = 0.526, 3/25 vs. 22/111 P = 0.572). Conclusion In terms of survival at one month, there was a significant difference between qSOFA score 1, 2, 3 and 4 subgroups. In patients with qSOFA score of 1 or 2 or 3, being under 50 years did not have a significant effect on one-month survival. Modified qSOFA may be beneficial to foresee the probable mortality but these findings need to be validated in larger cohorts Disclosures All authors: No reported disclosures.

scholarly journals WILSON’S DISEASE

2015 ◽  
Vol 22 (12) ◽  
pp. 1586-1590
Author(s):  
Sunil Kumar ◽  
Awais Bashir ◽  
Raheel Ahmed
Keyword(s):  
Wilson’S Disease ◽  
Tertiary Care ◽  
Care Facility ◽  
Wilson's Disease ◽  
P Value ◽  
Chi Square ◽  
Female Ratio ◽  
Male Predominance ◽  
Tertiary Care Facility ◽  
Chi Square Test

Objectives: To determine the frequency of various neurological manifestationsof Wilson’s disease in patients presenting to tertiary care facility. Study design: Cross sectionalstudy. Setting: Neurology ward, JPMC, Karachi. Period: 14-03-2012 to 13-09-2012. Patientsand Methods: A total of 150 diagnosed patients of Wilson’s disease between the age 15 and55 years were recruited. Structured questionnaire was used to collect the data regardingneurological manifestations of Wilson’s disease along with slit lamp examination for kayser-Fleischer rings. Data were entered and analyzed in SPSS version 17. Chi-Square test was usedas test of significance. Results: Mean age + S.D of patients was 38.7 + 8.23 years. Most of thepatients (42%) belongs to age between 41 to 50 years. Male to female ratio was 2:1. Tremorwas the most common neurological manifestation (88.7%) followed by dysarthria in 77.3%,dystonia was present in 59.3%, chorea in 36%, and Parkinsonism in 28.7%. All patients hadthe Kayser-Fleischer ring positive. Upon stratification of gender, tremor & dysarthria were morecommon in males as compared to females. Only dysarthria was significantly noted to increasewith increasing age up to 5th decade of life and decreased after age of 51 years. (P value< 0.001). Conclusion: Neurologic manifestations are very common in patients of Wilson’sdisease, tremor being the most common with male predominance.

scholarly journals Association of Acute Otitis Media with Breast Feeding Position Among Infants in a Tertiary Care Hospital of Pakistan

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
Samia Tul Rasool ◽  
Sana Mansoor ◽  
Tayyab Mumtaz Khan ◽  
Ashar Alamgir ◽  
Maida Meer ◽  
...  
Keyword(s):  
Otitis Media ◽  
Breast Feeding ◽  
Tertiary Care ◽  
Acute Otitis Media ◽  
P Value ◽  
Care Hospital ◽  
Chi Square ◽  
Educational Levels ◽  
Breastfed Infants ◽  
Chi Square Test

Acute otitis media (AOM) is the commonest disease of childhood. High incidence of AOM might be due to immature Eustachian Tube and immaturity of immune system. Exclusive breast feeding is protective against acute otitis media, however, improper breastfeeding  position can increase AOM occurrence in infants. This study aims to assess the association of improper breastfeeding position with occurrence of Acute otitis media in infants and to assess association of Maternal educational levels with feeding positions. 220 breastfed infants satisfying inclusion criteria with diagnosed AOM were enrolled. Study was conducted over a period of 6 months from August 2019 to January 2020. Demographic details and Detailed history regarding feeding positions and maternal educational levels were obtained through a Self-structured proforma from parents. Data analysis was done through descriptive statistics and chi square test and p value less than 0.5 was set significant. Out of 220 infants, 123(55.90%) were male and 97(44.10%) were females. AOM was more common in male infants than female infants, however it was not statistically significant(p=0.169). The mean age for enrolled infants(1-12months) was 6.90(±3.413). Statistically significant association between increase incidence of AOM and breastfeeding position was found(p=0.032). We also found a statistically significant association between maternal educational levels and breastfeeding position (p= 0.000). In this study, we have noted that Breastfeeding in supine position is associated significantly with increase in incidence of AOM. Feeding positions are statistically associated with Maternal Educational levels.

scholarly journals Clinical profile of pulmonary tuberculosis and MDR TB in children at tertiary medical institute

2019 ◽  
Vol 6 (6) ◽  
pp. 2571
Author(s):  
Anupama Vinayak Mauskar ◽  
Amrit Gopan
Keyword(s):  
Risk Factor ◽  
Pulmonary Tuberculosis ◽  
Tertiary Care ◽  
Admission Rate ◽  
P Value ◽  
Chi Square ◽  
Pulmonary Tb ◽  
Medical College ◽  
Mdr Tb ◽  
Chi Square Test

Background: India is the country with highest burden of TB. There is paucity of data as far as Pediatric TB is concern. TB in children directly reflects intensity of on-going transmission of TB in a given community. This study was done including indoor cases of Pediatric pulmonary TB in a medical college hospital, a tertiary care institute in the city of Mumbai. The aim and objectives of this study the clinical profile and outcome of Pediatric pulmonary tuberculosis/MDR TB in an indoor setting of a tertiary care center. It was a clinical observational study in a setting of medical college hospital.Methods: All admitted children with newly diagnosed pulmonary TB were included in study. A detailed clinical analysis was done. Statistical Analysis Association between two qualitative data was assessed by Chi-Square test, Fisher's exact test for all 2 X 2 tables where Chi-Square test was not valid due to small counts. Comparison of quantitative data measured between two outcomes was done using unpaired t-test. PSPP version 0.8.5 was used for statistical analysis.Results: Total of 41 patients with pulmonary TB were included in the study, making admission rate of 0.7% of total admission. Three out of 41 children had MDRTB making incidence 7% of total TB patients. Severe acute malnutrition was a major risk factor for dissemination of disease and mortality (p value 0.031and 0.0017).Conclusions: The study estimates 0.7% admission rate and 7% as incidence of MDRTB in indoor patients. Severe malnutrition was found to be risk factor for dissemination of disease [p value 0.031].

scholarly journals Comparative study of fetomaternal outcome in adolescent and young adult primigravidas

2017 ◽  
Vol 5 (3) ◽  
pp. 912
Author(s):  
Kanti Meherda ◽  
Shikha Mathur
Keyword(s):  
Young Adult ◽  
Adolescent Pregnancy ◽  
Economic Status ◽  
Tertiary Care ◽  
Public Health Problem ◽  
Adolescent And Young Adult ◽  
Control Group ◽  
P Value ◽  
Tertiary Care Centre ◽  
Chi Square

Background: Adolescent pregnancy is a worldwide public health problem. WHO has defined adolescence as the period from 10-19 years of age. Purpose of the study was to compare the fetomaternal outcome in adolescent and young adult primigravidas.Methods: The study was conducted at a tertiary care centre over a period of six months. 150 adolescent (in our study between 15-19 years of age) and 150 young adults (20-25 years) primigravidas who delivered at our institution were randomly selected for the study. All the data including age, booking status, educational and economic status and address were noted. All essential antepartum, intrapartum and postpartum data were collected for both the groups and compared using Chi square test.Results: In our study the incidence of antepartum, intrapartum and postpartum complications was 86%,36% and 10% respectively in the study group. But in the control group only 40% of the subjects had antepartum complications ,17.33% had intrapartum complications and the incidence of postpartum complications was only 4%. The difference is highly significant with a p value <0.001.Conclusions: Adolescent pregnancy is associated with adverse fetomaternal outcome and any effort to prevent it is worthwhile.

scholarly journals Effect of Carotid Stenting on Neurocognitive Function in Patients with Atherosclerotic Carotid Stenosis

2021 ◽  
Author(s):  
Brijesh Kumar Soni ◽  
Rajneesh Kumar Patel ◽  
Rochan Pant ◽  
Mukesh Khatri
Keyword(s):  
Carotid Stenosis ◽  
Tertiary Care ◽  
Study Groups ◽  
Carotid Stenting ◽  
Neurocognitive Function ◽  
Bivariate Analysis ◽  
P Value ◽  
Chi Square ◽  
Chi Square Test ◽  
Addenbrooke’S Cognitive Examination

Introduction: Stroke is among the leading cause of death worldwide. Carotid stenosis has been identified as a risk factor for stroke. The prevalence of carotid stenosis increases with age in both women and men. To reduce the risk of stroke, Carotid Endarterectomy (CEA) and Carotid Artery Stenting (CAS) are performed. Aim: To find the effect of carotid stenting on neurocognitive function in Atherosclerotic Carotid Stenosis. Materials and Methods: This prospective observational study was carried out at a tertiary care teaching hospital, over a 12-months duration. Twenty five patients were included in the study, after getting their informed consent. The patients underwent Addenbrooke’s cognitive examination preoperatively and were reassessed on 28 days and three months, postoperatively. The pre and postoperative scores were compared with the help of the paired t-test. Qualitative data were analysed with the help of frequency and percentage tables. Association among the study groups was assessed with the help of Bivariate analysis and Chi-square test and a p-value <0.05 was taken as significant. Results: The mean age of the patients was 65.52±9.34 years. Twenty two patients in the study group were male and three were female. Addenbrooke’s Score improved at 28 days and three months, postoperatively. There was a positive and significant correlation between preoperative Addenbrooke’s Score and postoperative Addenbrooke’s Score (r=0.748 and r=0.442, respectively). However, there was no statistically significant correlation noted with age, sex, and co-morbid condition like diabetes and hypertension and postoperative Addenbrooke’s score. Conclusion: The study showed that stent placement for patients with carotid stenosis improved the cognitive function.

Prevalence Of Post Partum Depression Among Postnatal Women At A Tertiary Care Centre Using Edinburgh Post Partum Depression Scale

2021 ◽  
Vol 12 (4) ◽  
pp. 2395-2399
Author(s):  
Sivasundari Maharajan ◽  
Dhanvarsha Sumaldha Subbiah Ramkumar ◽  
Nimrah Amthul ◽  
Shrutiravali
Keyword(s):  
Postpartum Depression ◽  
Negative Impact ◽  
Post Partum ◽  
Tertiary Care ◽  
Depression Scale ◽  
Medical Intervention ◽  
Tertiary Care Centre ◽  
Chi Square ◽  
Post Partum Depression ◽  
Chi Square Test

Postpartum depression is a grave mental health problem, which is more prevalent than society realizes and poses great risk. It has been associated with lack of breastfeeding, aversion towards the baby and negative impact on the development of the child. A total of 300 women who delivered between January 14 to March 31 of 2020 at Saveetha Medical College and Hospital were chosen following ethical clearance. After explaining the aspects of the Edinburgh Postpartum Depression Scale in their native language, the questionnaire was provided. A score of 10 and above were considered positive. Data were evaluated using SPSS and Chi square test. The prevalence of PPD was seen in 14% of women. It was higher with vaginal delivery, in women who had multiple births, lack of financial and social support, with domestic violence and alcoholic partner. Medical help was advised to women who scored 10 and above; only 5% sought medical intervention. Others did not recognize depression as a health issue. This shows the lack of awareness among the population. Medical health professionals should spread awareness, conduct more studies to evaluate the prevalence, the predisposing factors, methods for early diagnosis and ways to help women overcome “THE OTHER SIDE OF MOTHERHOOD”.

A knowledge, attitude and practice study on awareness of contraception among postpartum women in a tertiary care centre

2021 ◽  
Vol 12 (2) ◽  
pp. 1597-1601
Author(s):  
Sivasundari Maharajan ◽  
Dhanvarsha Sumaldha Subbiah Ramkumar ◽  
Perni Navya Sree
Keyword(s):  
Postpartum Period ◽  
Economic Status ◽  
Tertiary Care ◽  
Fetal Outcome ◽  
P Value ◽  
Postpartum Women ◽  
Tertiary Care Centre ◽  
Chi Square ◽  
Factors Affecting ◽  
Practice Methods

Despite being the first country to launch the National Family Planning Programme in 1952, India still lags behind in practising contraception. This study was conducted to assess knowledge, awareness and practice methods of contraception during the postpartum period. The study included 720 postpartum women; details including awareness and practice methods of contraception and factors affecting use by the postpartum women were obtained. Statistical analysis was done by using the chi-square test and percentage (%). The mean age of postpartum women was 26.5 years, 28.19% were literate between 9th - 12th grade, 96.94% women were housewives. Among them, 56% of women were aware of at least one contraception and correct usage. Among them, 98.01% knew about sterilization. About 48.88% of women were aware of more than one method of contraception. After counselling, 97.2% of women were willing to use contraception. However, 2.78% of patients chose abstinence. In our study, knowledge about contraception based on education and socio-economic status of women shows significant association (p-value <0.00001 for both). Health professionals should evaluate the level of awareness, create awareness and provide education regarding methods of contraception for use in the postpartum period to improve maternal and fetal outcome. 

scholarly journals Significance of Diet in Acne Vulgaris patients attending Dermatology clinic at Dow University of Health Sciences, Karachi

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
Badder Hina Afnan ◽  
Sadaf Ahmed Asim ◽  
Mehreen Amer ◽  
Sahar Soomro ◽  
Areesha Khan ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Dietary Habits ◽  
Acne Vulgaris ◽  
Tertiary Care ◽  
Glycemic Load ◽  
P Value ◽  
Care Hospital ◽  
Chi Square ◽  
Dermatology Clinic ◽  
Chi Square Test

BACKGROUND & OBJECTIVE: The pathology of acne is vaguely understood to date, current epidemiologic evidence in Eastern populations indicates that consumption of chocolate, milk intake, fatty products, and glycemic load may be involved. Previously little research has been conducted highlighting the possible relationship between diet and acne among patients in Pakistan. The objective of this study was to identify the association of acne vulgaris and dietary intake patterns. METHODOLOGY:  An observational study enrolled 246 participants, both genders, 16 to 40 years of age, diagnosed with Acne Vulgaris recruited from a tertiary-care hospital out-patient clinic in Karachi. The structured questionnaire consisted of various methods for assessing nutritional parameters.  Statistical analysis was carried out using SPSS, the association of Acne was tested with dietary habits using Pearson-Chi Square test. Food items with a p-value less than 0.05 in the Chi Square test were considered in Binary logistic regression for estimating the risk for Acne with a 95% confidence interval (C.I.). RESULTS: Fifty-two percent of participants were found with mild acne, use of yogurt, ice cream butter, margarine, French fries, chocolate, bakery chips, white bread, vegetable, fruits, cake dates, and pickles give a significant association with severity of acne using Fisher's Exact test p<0.05. Logistic regression showed that butter gave more risk for acne with an odds ratio of 1.11 and 95% C.I. (1.0, 9.2).  CONCLUSION: It can be stated that dairy products, high glycemic index, and fatty foods are associated with acne aggravation in our population. As patients are always concerned about dietary advice, suggesting that they reduce these products would help them maintain their skin.

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