scholarly journals Micorrizas arbusculares no crescimento de mudas de sabiá em um substrato proveniente da mineração de manganês

Author(s):  
Kaio Gráculo Vieira Garcia ◽  
Vânia Felipe Freire Gomes ◽  
Aldênia Mendes Mascena de Almeida ◽  
Paulo Furtado Mendes Filho

<p>O manganês (Mn) é considerado um micronutriente essencial às plantas, porém o seu excesso pode resultar em toxidez, causando clorose e necrose nas folhas. O uso de leguminosas inoculadas com fungos micorrízicos arbusculares tem se tornado uma importante estratégia do ponto de vista da remediação de áreas contaminadas com metais como é o caso do manganês. Neste sentido, o objetivo deste trabalho foi avaliar o crescimento de mudas de <em>Mimosa caesalpiniaefolia</em> Benth. inoculadas com micorrizas arbusculares sob condições de substrato esterilizado e natural proveniente de uma área de mineração de Mn do município de Ocara – CE. O experimento foi conduzido em casa de vegetação, utilizando-se o delineamento inteiramente casualizado, em esquema fatorial 4x2, considerando-se: quatro tratamentos de inoculação (controle não-inoculado, inoculado com <em>Glomus clarum</em>, inoculado com <em>Glomus etunicatum</em> e com a mistura dos dois fungos (Mix)) e duas condições de substrato (estéril e natural), com quatro repetições. As mudas de sabiá foram produzidas em bandeja de isopor, sendo transplantadas e inoculadas em vasos após treze dias da semeadura. Após trinta dias do transplantio foram realizadas avaliações de altura da parte áerea, diâmetro do colo, número de folíolos e porcentagem de sobrevivência. A inoculação com fungos micorrízicos arbusculares favoreceu o crescimento das mudas de sabiá em substrato da mineração de manganês, em condições naturais, principalmente quando se utilizou o <em>Glomus etunicatum</em>. No substrato esterilizado a inoculação com micorrizas arbusculares proporcionou a atenuação da toxidez de Mn nas mudas de sabiá favorecendo o crescimento das mesmas nestas condições.</p><p align="center"><strong><em>Arbuscular mycorrhiza on the growth of sabiá seedlings on a substrate from the manganese mining</em></strong></p><p><strong>Abstract:</strong> Manganese (Mn) is considered an essential micronutrient to plants but its excess can result in toxicity causing chlorosis and necrosis on the leaves.  The use of leguminous plants inoculated with mycorrhizal fungi have become an important point of view of the remediation strategy contaminated with metals such as manganese.  In this sense, the objective of this study was to evaluate the growth of <em>Mimosa caesalpiniaefolia</em> Benth. seedlings inoculated with mycorrhiza under conditions of sterile and natural substrate from a Mn mining area of the municipality of Ocara - CE.  The experiment was conducted in a greenhouse using a completely randomized design in a 4x2 factorial scheme considering four inoculation treatments (non-inoculated control, inoculated with <em>Glomus clarum</em>, inoculated with <em>Glomus etunicatum</em> and with the mixture of two fungi (Mix)) and two substrate conditions (sterile and natural), with four replications.  The seedlings of sabiá were produced in styrofoam tray, being transplanted and inoculated in pots after thirteen days of sowing.  After thirty days of transplanting been evaluated shoot height, stem diameter, number of leaflets and percentage of survival.  Inoculation with mycorrhizal fungi favored the growth of sabiá seedlings in manganese mining substrate, in natural conditions, especially when using the <em>Glomus etunicatum</em>.  In the substrate sterilized inoculation with mycorrhiza provided attenuation of Mn toxicity in sabiá seedlings favoring their growth under these conditions.</p>

2010 ◽  
Vol 82 (3) ◽  
pp. 771-777 ◽  
Author(s):  
Edson L. Souchie ◽  
Rosario Azcón ◽  
Jose M. Barea ◽  
Eliane M.R. Silva ◽  
Orivaldo J. Saggin-Júnior

This study evaluated the synergism between several P-solubilizing fungi isolates and arbuscular mycorrhizal fungi to improve clover ( Trifolium pratense) growth in the presence of Araxá apatite. Clover was sown directly in plastic pots with 300g of sterilized washed sand, vermiculite and sepiolite 1:1:1 (v:v:v) as substrate, and grown in a controlled environment chamber. The substrate was fertilized with 3 g L-1 of Araxá apatite. A completely randomized design, in 8×2 factorial scheme (eight P-solubilizing fungi treatments with or without arbuscular mycorrhizal fungi)and four replicates were used. The P-solubilizing fungi treatments consisted of five Brazilian P-solubilizing fungi isolates (PSF 7, 9, 20, 21 and 22), two Spanish isolates ( Aspergillus niger and the yeast Yarowia lipolytica) and control (non-inoculated treatment). The greatest clover growth rate was recorded when Aspergillus niger and PSF 21 were co-inoculated with arbuscular mycorrhizal fungi. Aspergillus niger, PSF 7 and PSF 21 were the most effective isolates on increasing clover growth in the presence of arbuscular mycorrhizal fungi. Greater mycorrhizal colonization resulted in greater clover growth rate in most PSF treatments. PSF 7 was the best isolate to improve the establishment of mycorrhizal and rhizobia symbiosis.


2020 ◽  
Vol 17 (4) ◽  
pp. 150
Author(s):  
O. TRISILAWATI

<p>ABSTRACT</p><p>The effects of several arbuscular mycorrhizal fungi (AMF) on thegrowth, nutrient uptake (nitrogen, phosphorus, and potassium), and acidphosphate activity of two promising numbers of Anacardium occidentaleseedling were evaluated. The experiment was conducted in the greenhouse of Indonesian Spices and Medicinal Crops Research Institute(BALITTRO) in 2002 for six months on a randomized design with twofactors and four replicates. First factor was isolate (six isolates of AMFand one control) consisting of : 1) control; 2) Glomus aggregatum; 3)Glomus etunicatum; 4) Mycofer; 5) Glomus sp.; 6) a mixture of Glomussp1, Glomus sp2, Glomus sp3, Glomus sp4, Glomus etunicatum,Gigaspora margarita, Gigaspora sp., and Enthropospora sp., and 7)Gigaspora sp. The second factor was two cashew promising numbers :Asembagus and Wonogiri. The results showed that AMF inoculationsignificantly affected the growth of cashew. Mycofer and mixed AMFwere more effective to Wonogiri promising number, while for Asembaguspromising number inoculation of mycofer was more effective. Inoculationwith mycofer to Asembagus promising number increased the uptake of Pand K nutrients by 65 and 53% while inoculation with mycofer and mixedAMF to Wonogiri promising number increased the uptake of N, P and Knutrients by 55, 38, and 17%, and by 18, 31, and 17%. Moreover, theAMF inoculation resulted in higher phosphatase activity. In mycorrhizalAsembagus promising number infected by mixed AMF, the increment ofphosphatase activity was 136.5%, whether in Wonogiri promising numberinfected by mycofer, the increment of phosphatase activity was 80% thancontrol.</p><p>Key words: Anacardium occidentale, promising number, growth,phosphatase activity</p><p>ABSTRAK</p><p>Pengaruh Pupuk Hayati Fungi Mikoriza Arbuskula(FMA) terhadap Pertumbuhan Benih Jambu Mete</p><p>Penelitian ini bertujuan untuk mengetahui pengaruh beberapa jenisfungi mikoriza arbuskula (FMA) terhadap pertumbuhan, serapan hara danaktivitas enzim fosfatase dari dua nomor harapan benih jambu mete(Anacardium occidentale). Penelitian dilakukan di rumah kaca Balittropada tahun 2002 selama 6 bulan, menggunakan rancangan acak yangterdiri dari dua faktor dan diulang empat kali. Faktor pertama adalahisolate (6 jenis isolat FMA dan satu kontrol) yaitu: 1). kontrol; 2). Glomusaggregatum; 3) Glomus etunicatum; 4). Mycofer; 5). Glomus sp.; 6).campuran dari Glomus sp1, Glomus sp2, Glomus sp3, Glomus sp4, Glomusetunicatum, Gigaspora margarita, Gigaspora sp., Enthropospora sp., dan7). Gigaspora sp. Faktor kedua adalah nomor harapan jambu mete, yaituAsembagus dan Wonogiri. Hasil penelitian mendapatkan bahwa inokulasiFMA berpengaruh nyata terhadap pertumbuhan jambu mete. Mycofer dancampuran FMA lebih efektif berpengaruh terhadap nomor harapanWonogiri, sedangkan mycofer lebih efektif berpengaruh terhadap nomorharapan Asembagus. Serapan hara P dan K pada nomor harapanAsembagus yang diinokulasi mycofer meningkat sebesar 65 dan 53%,sedangkan nomor harapan Wonogiri yang diinokulasi mycofer dancampuran FMA, serapan hara N, P, dan K meningkat masing-masingsebesar 55; 38; dan 17%, dan 18; 31; dan 17%. Selain itu, inokulasi FMAdapat meningkatkan aktivitas fosfatase akar jambu mete. Peningkatanaktivitas fosfatase akar jambu mete nomor harapan Asembagus yangterinfeksi oleh campuran FMA sebesar 136,5%, sedangkan pada nomorharapan Wonogiri yang terinfeksi mycofer, peningkatnnya sebesar 80%dibandingkan kontrol.</p><p>Kata kunci: Anacardium occidentale, nomor harapan, pertumbuhan,aktivitas fosfatase</p>


Akta Agrosia ◽  
2016 ◽  
Vol 19 (1) ◽  
pp. 20-27
Author(s):  
Tri Utami ◽  
Hermansyah Hermansyah ◽  
Merakati Handajaningsih

The purpose of this study was to determine the optimum concentration of shallot extract to the growth of grape cuttings grape cuttings. This study was conducted from September to December 2015 in the city of Bengkulu. This research used Completely Randomized Design with a single factor and five replications. Treatment concentrations of 0% (control), shallot extract concentration of 25%, 75% and 100%. The variables measured were the time to sprout, shoot height, the number of leaves, number of roots, fresh weight and dry weight of cuttings. The results showed that the concentration of shallot extract 100% concentration enhances the growth of grape cutting, time to sprout, and the concentration of extract 50% increase shoot height and number of leaves on grape cuttings.


DYNA ◽  
2020 ◽  
Vol 87 (214) ◽  
pp. 85-92
Author(s):  
Raimundo Fernandes de Brito ◽  
Miguel Ferreira Neto ◽  
Jonath Werissimo Da Silva Gomes ◽  
Ana Luíza Lima Ferreira ◽  
Nildo Da Silva Dias ◽  
...  

In this study, we evaluated the effects of fertigation with domestic sewage effluent and different substrates on the growth of Caraíba seedlings (Tabebuia aurea Benth). The experiment was accomplished in a greenhouse in Mossoró, RN. Five irrigation solutions (100% of water supply - WS, 100% of domestic sewage effluent – DS and dilutions of 75% DS + 25% WS, 50% DS + 50% WS e 25% DS + 75% WS) were tested and two substrates (75% soil + 25% bovine manure and 75% soil + 25% coconut fiber) using completely randomized design in subdivided plots with three replicates per treatment. Growth and development parameters were measured at 30, 60, 90, and 150 days after cultivation. The variables shoot height, collar diameter, height/diameter ratio, dry matter of root, shoot and total (root and shoot), shoot/root ratio, Dickson quality index, and nutritional evaluation of nitrogen, phosphorus, potassium and calcium were determinate. We verified that the use of domestic sewage increased growth and quality of caraíba seedlings, besides promoting nutrient accumulation in the plants grown in substrate of bovine manure plus soil with 100% application of domestic sewage in the fertigation.


CERNE ◽  
2013 ◽  
Vol 19 (2) ◽  
pp. 289-295 ◽  
Author(s):  
Sílvio Júnio Ramos ◽  
Valdemar Faquin ◽  
Fabrício William Ávila ◽  
Rose Myrian Alves Ferreira ◽  
Josinaldo Lopes Araújo

Soils destined for forest crops are often limiting with respect to boron (B) and in several eucalyptus crops boron deficiency has been found to be a common occurrence. The objective of this study is to evaluate biomass production, B accumulation and Ca/B ratio in eucalyptus cultivated under different conditions of soil, water availability and doses of B. To that end, an experiment was conducted using a completely randomized design with a 5 x 2 x 2 factorial arrangement consisting of five doses of B (0.00, 0.25, 0.75, 2.25 and 6.25 mg kg-1), two types of soil, namely a Dark Red Latosol (LE) and a Red-Yellow Latosol (LV), and two water tension levels (-0.033 and -0.010 MPa), with four replicates. After plants were grown, determinations were made of Ca and B contents as well as Ca/B ratios in different portions of the plants. Accumulation of B was obtained from the ratio of its contents to dry biomass in each plant portion. It was found that, under natural conditions, both Dark Red Latosol and Red-Yellow Latosol fail to meet the nutritional requirements for B in eucalyptus, potentially being limiting to that species. Overall, soil moisture influenced dry biomass production and accumulation of B only in the case of the more sandy texture soil (Red-Yellow Latosol), denoting that this factor is conditional on soil texture. Ca/B ratio results showed that application of B to the soil without a suitable supply of Ca can lead to an imbalance between these nutrients, with possible implications for plant growth and nutrition.


2010 ◽  
Vol 55 (1) ◽  
pp. 17-28
Author(s):  
M.B. Adewole ◽  
O.O. Awotoye ◽  
M.O. Ohiembor ◽  
A.O. Salami

The influence of mycorrhizal fungi in uptake of heavy metals, pollution response index and yield of sunflower in degraded soils were investigated. It was a greenhouse experiment with 2 arbuscular mycorrhizae (Glomus mosseae, Glomus intraradices) and a non-inoculation that served as control. The treatments were replicated 3 times in a completely randomized design. Each of the treatment consisted of 30 pots and each pot was filled with 5 kg by weight of dried top soil. Solutions of lead acetate and cadmium sulphate at variable levels of: 0, 250, 500, 750, 1000 mg kg-1 and 0, 20, 40, 60, 80 mg kg-1 respectively were used to pollute the soils. Increase in pollution-stressed conditions significantly (P<0.05) reduced the infection of sunflower roots, and the uptake of Pb and Cd in the dry root of sunflower was also significantly (P<0.05) reduced. Also, arbuscular mycorrhizae enhanced the root infection of sunflower, increased the pollution tolerance and consequently increased the yield of sunflower.


2020 ◽  
Vol 1 (38) ◽  
pp. 89-95
Author(s):  
Chi Thi Le Diep ◽  
Nho Thi Vo

This study aimed to determine the effects of infrared lamps on survival rates of mature and immature common sun skinks (Eutropis multifasciata) in semi-natural conditions in Quang Binh Province, Vietnam, in the cold weather. A sample size of 400 mature (28 weeks old) and 120 immature common sun skinks were used in a completely randomized design with two variables of different lighting methods: infrared and incandescent light. Suitable food and water were freely provided. Results showed that the survival rates of the mature and juvenile common sun skinks were 82.5% and 39.2% with infrared light and 30.5% and 0% with incandescent light. Compared to incandescent light, the growth of young offsprings in the first 4 weeks after birth was greater with the infrared light and the average cumulative growth weight reached 2.18 g per individualby the fourth week. With incandescent light, common sun skinks lost their weight over time and gradually died out by the third week. Thus, infrared light which can be used to warm up pens may be an effective techniquein raising common sun skinks in semi-natural conditions in Quang Binh.


2015 ◽  
Vol 50 (4) ◽  
pp. 313-321 ◽  
Author(s):  
Marco Aurélio Carbone Carneiro ◽  
Dorotéia Alves Ferreira ◽  
Edicarlos Damacena de Souza ◽  
Helder Barbosa Paulino ◽  
Orivaldo José Saggin Junior ◽  
...  

The objective of this work was to evaluate the spore density and diversity of arbuscular mycorrhizal fungi (AMF) in soil aggregates from fields of "murundus" (large mounds of soil) in areas converted and not converted to agriculture. The experiment was conducted in a completely randomized design with five replicates, in a 5x3 factorial arrangement: five areas and three aggregate classes (macro-, meso-, and microaggregates). The evaluated variables were: spore density and diversity of AMF, total glomalin, total organic carbon (TOC), total extraradical mycelium (TEM), and geometric mean diameter (GMD) of soil aggregates. A total of 21 AMF species was identified. Spore density varied from 29 to 606 spores per 50 mL of soil and was higher in microaggregates and in the area with 6 years of conversion to agriculture. Total glomalin was higher between murundus in all studied aggregate classes. The area with 6 years showed lower concentration of TOC in macroaggregates (8.6 g kg-1) and in microaggregates (10.1 g kg-1). TEM was greater at the top of the murundus in all aggregate classes. GMD increased with the conversion time to agriculture. The density and diversity of arbuscular mycorrhizal spores change with the conversion of fields of murundus into agriculture.


2019 ◽  
Vol 24 (2) ◽  
pp. 143-152
Author(s):  
Marhan Nurullia ◽  
Erni Suminar Suminar ◽  
Anne Nurani

This study was aimed at determining the response of turmeric shoot explants after the provision of various types and concentrations of cytokinins in vitro. This experiment was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Padjadjaran University from January to April 2018. The data were analyzed using T-Test. The experimental method used in this research was Completely Randomized Design (CRD). Explant planting was carried out in Laminar Air Flow. The experiment consisted of 7 treatments consisting of 4 replications and each test consisted of 4 units. Observation of this experiment was carried out for 12 MST. The main observations were made on the data that were tested statistically namely the percentage of explant growing shoots, percentage of explant growing roots, shoot height, number of tuns, number of roots and root length. The treatments consisted of Control, 2.5 mg L-1 BAP, 5 mg L-1 BAP, 0.5 mg L-1 TDZ, 1 mg L-1 TDZ, 0.01 mg L-1 Zeatin and 0.1 mg L Zeatin -1. The results show that the treatment of 1 mg L-1 TDZ shows the best response to the growth of turmeric explants by increasing the number of turmeric shoot explants than the others.RESPONS EKSPLAN TUNAS KUNYIT SETELAH SITOKININ SECARA IN VITROTujuan dari penelitian ini yaitu untuk melihat respons eksplan tunas kunyit terhadap pemberian berbagai jenis dan konsentrasi sitokinin secara in vitro. Percobaan ini dilakukan di Laboratorium Kultur Jaringan, Fakultas Pertanian, Universitas Padjadjaran dari bulan Januari sampai April 2018. Hasil percobaan dianalisis dengan Sample T-Test. Metode percobaan yang digunakan dalam penelitian ini yaitu Rancangan Acak Lengkap (RAL). Penanaman eksplan dilakukan di dalam Laminar Air Flow. Percobaan terdiri dari 7 perlakuan sebanyak 4 ulangan dan setiap ulangan terdiri dari 4 unit. Pengamatan percobaan ini dilakukan selama 12 MST. Pengamatan utama dilakukan terhadap data-data yang diuji secara statistik yakni persentase eksplan tumbuh tunas, persentse eksplan tumbuh akar, tinggi tunas, jumlah tunas, jumlah akar dan panjang akar. Perlakuan terdiri dari Kontrol; 2,5 mg L-1 BAP; 5 mg L-1 BAP; 0,5 mg L-1 TDZ; 1 mg L-1 TDZ; 0,01 mg L-1 Zeatin; dan 0,1 mg L-1 Zeatin. Hasil penelitian menunjukkan bahwa perlakuan 1 mg L-1 TDZ menunjukkan respons yang lebih baik terhadap pertumbuhan eksplan kunyit dengan meningkatkan jumlah tunas eksplan tanaman kunyit daripada yang lainnya.


2018 ◽  
Vol 31 (3) ◽  
pp. 602-611 ◽  
Author(s):  
JESSICA SILVA SANTOS ◽  
JACILENE FRANCISCA SOUZA SANTOS ◽  
LÁZARA JOSSIKARLA DE OLIVEIRA LOPES ◽  
JOHNY DE JESUS MENDONÇA ◽  
FRANCISCO SANDRO RODRIGUES HOLANDA ◽  
...  

ABSTRACT Vetiver grass is a member of the grass family Poaceae. Its fast development is probably due to the interaction with native microbiota, whose influence has not been studied yet. The objective of this work was to evaluate the colonization and development of the vetiver grass (Chrysopogon zizanioides (L.) Roberty) inoculated with arbuscular mycorrhizal fungi and dark septate endophytic fungi. The experimental design was a completely randomized design with six treatments (control, without mycorrhizal fungi, native inoculants, UFLA05 - Gigaspora albida, UFLA351 - Rhizoglomus clarum, UFLA372 - Claroideoglomus etunicatum, and UFLA401 - Acaulospora morrowiae), with three replicates each. Vetiver grass tillers as well as the native microbial inoculum were obtained from the Lower São Francisco river experimental area, located in Sergipe state, Northeastern Brazil. There was a negative interaction between all tested UFLAs mycorrhizal isolates and the native microbiota (mycorrhizal and endophytic fungi) in the treatments, especially when taking into consideration plant height and volume of roots. The effects of inoculation with UFLA isolates may have been influenced by the presence of the native mycorrhizal fungi and the dark septate endophytic fungi. Vetiver grass was responsive to the native inoculant. The mycorrhizal colonization of the vetiver grass was vesicular, but the formation of the arbuscules can be influenced by the interaction between the fungus, plant, and the environment.


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