scholarly journals Epidemiological characterization of dermatophytes at a tertiary care hospital in Eastern Uttar Pradesh, India

2019 ◽  
Author(s):  
Ankur Kumar ◽  
Vandana Upadhyay ◽  
Amresh K. Singh ◽  
Jayesh Pandey
Keyword(s):  
Low Socioeconomic Status ◽  
Fungal Infections ◽  
Tertiary Care ◽  
Uttar Pradesh ◽  
Common Species ◽  
Personal Hygiene ◽  
Clinical Samples ◽  
Care Hospital ◽  
Direct Microscopic Examination ◽  
Superficial Mycosis

Background and Purpose: Superficial mycosis is more prevalent in tropical and subtropical countries, such as India. Regarding this, the present study was conducted to determine the epidemiology of superficial mycosis and identify the most common dermatophytic species in this region. Materials and Methods: For the purpose of the study, a total of 220 skin scraping, nail, and hair root specimens were collected. Direct microscopic examination was performed using potassium hydroxide mount. Additionally, the samples were inoculated onto Sabouraud dextrose agar (SDA) and dermatophyte test medium (DTM). The fungal colony of each isolates was stained with lactophenol cotton blue mount, and observed under microscope for species identification. Results: Out of 220 isolates, 172 samples, obtained from 108 males 64 females, were positive for skin fungal infections by either KOH mount or culture. Furthermore, 113 isolates were identified as dermatophytes, while 59 samples were found to be non-dermatophytes. Among the dermatophytes isolated from different clinical samples, Trichophyton verrucosum (42/113, 38%) was the most common species, and Tinea corporis was the most common infection (36.2%). Conclusion: As the findings indicated, dermatophytes had an isolation rate of 78%, which is higher than normal. This can be due to the fact that the majority of the patients were from a rural background (71.7%) with a low socioeconomic status and poor personal hygiene who were exposed to climatic changes.

scholarly journals Spectrum of Opportunistic Fungal Infections in HIV/AIDS Patients in Tertiary Care Hospital in India

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Ravinder Kaur ◽  
Megh S. Dhakad ◽  
Ritu Goyal ◽  
Preena Bhalla ◽  
Richa Dewan
Keyword(s):  
Fungal Infections ◽  
Tertiary Care ◽  
North India ◽  
Clinical Samples ◽  
Fungal Culture ◽  
Care Hospital ◽  
Aids Patients ◽  
Cell Counts ◽  
Fungal Isolates ◽  
Hiv Aids

HIV related opportunistic fungal infections (OFIs) continue to cause morbidity and mortality in HIV infected patients. The objective for this prospective study is to elucidate the prevalence and spectrum of common OFIs in HIV/AIDS patients in north India. Relevant clinical samples were collected from symptomatic HIV positive patients (n=280) of all age groups and both sexes and subjected to direct microscopy and fungal culture. Identification as well as speciation of the fungal isolates was done as per the standard recommended methods. CD4+T cell counts were determined by flow cytometry using Fluorescent Activated Cell Sorter Count system. 215 fungal isolates were isolated with the isolation rate of 41.1%.Candidaspecies (86.5%) were the commonest followed byAspergillus(6.5%),Cryptococcus(3.3%),Penicillium(1.9%), andAlternariaandRhodotorulaspp. (0.9% each). AmongCandidaspecies,Candida albicans(75.8%) was the most prevalent species followed byC. tropicalis(9.7%),C. krusei(6.4%),C. glabrata(4.3%),C. parapsilosis(2.7%), andC. kefyr(1.1%). Study demonstrates that the oropharyngeal candidiasis is the commonest among different OFIs and would help to increase the awareness of clinicians in diagnosis and early treatment of these infections helping in the proper management of the patients especially in resource limited countries like ours.

scholarly journals Mycological Pattern of Dermatomycoses in a Tertiary Care Hospital

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Ravinder Kaur ◽  
Pragyan Swagatika Panda ◽  
Kabir Sardana ◽  
Sahanawaj Khan
Keyword(s):  
Trichophyton Rubrum ◽  
Fungal Infections ◽  
Indian Subcontinent ◽  
Tertiary Care ◽  
North India ◽  
Clinical Samples ◽  
Care Hospital ◽  
Disease Occurrence ◽  
Normal Laboratory

Background. Dermatomycoses are not diseases requiring compulsory notifications; rather they cause cosmetic defacements. Indian subcontinent with a varied topography is favorable for various fungal infections.Objective. To look for the epidemiological and mycological profile of superficial mycoses in North India.Methods. Three hundred and fifty-one clinical samples of skin, hair, and nail were examined to find the fungal etiology of the dermatomycoses.Results. Dermatomycoses were seen in 215/351 (61.2%) of cases. Most common isolates obtained were nondermatophyte molds (NDMs) (36.1%), followed by dermatophytes (13.8%) and yeasts (8.6%).Aspergillus niger(9%) was the most common mold.Trichophyton rubrum(4.6%) was the most common dermatophyte isolated, while amongst the yeastsNon-albicans Candida(NAC) species were more common (6%). Many other NDMs likeSyncephalastrumspp.,Cunninghamellaspp.,Rhodotorulaspp.,A. terreus, Scytalidiumspp. andScopulariopsisspp. were also isolated.Conclusion. Our study reflects an increasing role of NDMs (thought to be normal laboratory or environmental contaminants) as a causative agent of dermatomycoses, replacing the dermatophytes. Clinician’s awareness of the demographic profile of the population involved along with more studies on dermatomycoses can help in understanding the etiological profile in area, leading to prevention of disease occurrence and cosmetic disfigurement.

scholarly journals Clinicomycological evaluation of dermatophytosis at tertiary care hospital in central India

2018 ◽  
Vol 4 (3) ◽  
pp. 409
Author(s):  
Shyam Govind Rathoriya ◽  
Ankit Kumar Jain ◽  
Kavita A. Shinde
Keyword(s):  
Fungal Infections ◽  
Tertiary Care ◽  
Age Groups ◽  
Central India ◽  
Common Species ◽  
Tinea Corporis ◽  
Valuable Insight ◽  
Care Hospital ◽  
Cross Sectional ◽  
Tinea Cruris

<p class="abstract"><strong>Background:</strong> Dermatophytoses are the infection of keratinized tissues such as the epidermis, hair, and nails caused by a group of closely related filamentous fungi known as dermatophytes.</p><p class="abstract"><strong>Methods:</strong> It was a hospital based cross-sectional study. A total number of 150 clinically diagnosed cases of skin, hair and nail infections were randomly selected from all the age groups and of both the sexes, attending Dermatology Outpatient department of CMCH, Bhopal from January 2016-December 2017.<strong></strong></p><p class="abstract"><strong>Results:</strong> Out of total 150 subjects, most common clinical type of dermatophytosis, identified in our study, was tinea corporis in 53 (35.3%) subjects followed by tinea cruris in 34 (22.6%) subjects. 134 (89.3%) subjects were tested positive by direct microscopy (KOH mount) and 69 (46.0%) by culture. Highest KOH mount positivity was seen in patient suffering from tinea corporis (94.3%) followed by tinea cruris (94.1%). Culture positivity was highest with tinea corporis (54.7%) followed by tinea lesions on more than one site (47.3%) and tinea cruris (47.0%). In our study, total 69 culture positive samples were isolated and the most common species isolated was T. rubrum in 41 (59.42%) cases.</p><p><strong>Conclusions:</strong> The present study gives valuable insight regarding clinical and mycological pattern of superficial fungal infections in this region as well as shows the importance of mycological examination of dermatophytosis samples for planning effective management. </p>

scholarly journals Studying the clinic mycological pattern of the dermatophytic infection attending OPD in tertiary care hospital in eastern Uttar Pradesh and Bihar

2018 ◽  
Vol 4 (2) ◽  
pp. 118 ◽  
Author(s):  
Anil Kumar Gupta ◽  
Aayushi Mohan ◽  
Santosh Kumar Singh ◽  
Amit Kumar Pandey
Keyword(s):  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Uttar Pradesh ◽  
Common Species ◽  
Outpatient Department ◽  
Care Hospital ◽  
Sample Collection ◽  
Female Ratio ◽  
Male Female Ratio ◽  
Eastern Uttar Pradesh

<p class="abstract"><strong>Background:</strong> Superficial dermatophytic infection is infection of skin nail or hair with fungus. Nowadays, these fungal infection are at a rise and run a prolong course despite of treatment due to resistance to conventional antifungal agents. There is a felt need to conduct epidemiological study to know the change in the pattern and cause of widespread resistance. This study was aimed at identifying clinico-mycological pattern of dermatophytic infections in patients attending the dermatology outpatient department of a tertiary care hospital in eastern Uttar Pradesh and adjoining area.</p><p class="abstract"><strong>Methods:</strong> Patients with suspected dermatophytoses attending the outpatient department were enrolled in the study. A detailed history, clinical examination and sample collection for mycological examinations was done.<strong></strong></p><p class="abstract"><strong>Results:</strong> There were 500 patients recruited in the study, with a male: female ratio of 3:1. The most commonly affected age group was 20–30 years (35%). Tinea corporis et cruris was the most common type observed (31%). Potassium hydroxide positivity was seen in 390 samples (78%) and culture positivity was found in 350 samples (70%). The most common species identified was <em>Trichophyton verrucosum</em> (35.5%).</p><p><strong>Conclusions:</strong> There is a rise in dermatophytic infection caused by zoophilic species like <em>Trichophyton verrucousum</em>.</p>

scholarly journals An Update on Secondary Bacterial and Fungal Infections and Their Antimicrobial Resistance Pattern (AMR) in COVID-19 Confirmed Patients at a Tertiary Care Hospital

2022 ◽  
Author(s):  
Sushma Yadav Boorgula ◽  
Sadhana Yelamanchili ◽  
Pragathi Kottapalli ◽  
Mohini D. Naga
Keyword(s):  
Antimicrobial Resistance ◽  
Fungal Infections ◽  
Treatment Options ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
P Value ◽  
Care Hospital ◽  
Stewardship Program ◽  
Secondary Infections

Abstract Introduction Since coronavirus disease 2019 (COVID-19) has limited treatment options, concern has been raised over secondary infections and antimicrobial resistance (AMR) patterns. It has been observed that patients who were infected with COVID-19 were predisposed to develop secondary infections. The purpose of the study is to ascertain the prevalence of the bacterial and fungal coinfections in COVID-19 patients, and also to assess the AMR patterns in the obtained isolates. Methods We have studied 200 clinical samples obtained from 122 COVID-19 positive patients. Pathogens were identified using Vitek 2 system. The demographic and clinical patterns were also observed. Results A total of 122 patients developed secondary infections. Patients aged more than 40 years were majorly affected (p-value < 0.0001). Respiratory samples (n = 96) were predominant. Klebsiella pneumoniae (n = 68) was the most common pathogen isolated followed by Acinetobacter baumannii (n = 54) and an overall 6% rise in the Carbapenem resistance was observed in the isolates. Conclusion To contain the secondary infections in COVID-19 patients, it is imperative to adhere to antimicrobial stewardship program and timely revise the empirical antibiotic policy.

scholarly journals Epidemiological profile of candidiasis in HIV/AIDS patients in a tertiary care hospital

2020 ◽  
Vol 7 (12) ◽  
pp. 4858
Author(s):  
Megh S. Dhakad ◽  
Ravinder Kaur ◽  
Ritu Goyal ◽  
Preena Bhalla ◽  
Richa Dewan
Keyword(s):  
Fungal Infections ◽  
Oral Candidiasis ◽  
Tertiary Care ◽  
Cd4 Count ◽  
Clinical Samples ◽  
Care Hospital ◽  
Cd4 Counts ◽  
Epidemiological Profile ◽  
Loss Of Appetite ◽  
Hiv Aids

Background: Clinical research in fungal infections is largely a neglected area in health care settings in India. Candida species cause diseases ranging from mucosal infections to systemic mycoses depending on host’s immune status. Aim of this study was to evaluate the clinico-epidemiological profile of candidiasis, and their correlation with an immunological profile in HIV/AIDS patients.Methods: Clinical details and investigations of 200 symptomatic, confirmed HIV-positive patients, suspected of having candidiasis were recorded and analyzed. Relevant clinical samples depending on the organ system involved were collected and subjected to direct microscopy, culture isolation and serology. Identification and speciation of the isolates was done by biochemical methods as per standard recommended procedures. CD4-count was determined by flow cytometry using Fluorescent Activated Cell Sorter Count system.Results: Patients ranged from 17-65 years with a mean age of 33.83±9.07 years. Most common clinical presentations were seen to be white oral patches (82%), weight loss (79%), fever (67%), loss of appetite (53%), headache (51.5%), cough (38.5%) and diarrhea (30%).  Gastrointestinal system (35%) was the most commonly involved system. The CD4-counts ranged from 16-1033 cells/μl. 93 (46.5%) patients had CD4-counts <200 cells/μl, while CD4-count <100 cells/μl was seen in 40 (20%) and CD4-count <50 cells/μl in 20 (10%). Candidiasis was detected in 60% of the patients. Yeasts isolated were C. albicans (82.51%), C. tropicalis (6.29%), C. krusei (4.89%), C. parapsilosis (3.49%), and C. glabrata (2.79%).Conclusions: C. albicans was predominant species and presence of oral candidiasis is a matter of concern. Early and accurate diagnosis of candidiasis is one of the keys helps for the success of effective HIV/AIDS disease management. 

scholarly journals SPECTRUM OF FUNGAL INFECTIONS IN THE ELDERLY AGE GROUP

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Kshitij Saxena ◽  
Priyanka Shukla ◽  
Harris Ishtiyaq Shaafie ◽  
Garav Palliwal ◽  
Chandni Jain
Keyword(s):  
Fungal Infections ◽  
Tertiary Care ◽  
The Elderly ◽  
Hair Follicles ◽  
Sweat Glands ◽  
Care Hospital ◽  
Age Group ◽  
Elderly Age ◽  
Direct Microscopy ◽  
Superficial Mycosis

ABSTRACT INTRODUCTION: Fungal infections have become a common problem in the elderly age group. As age advances, the skin becomes dry, thin, wrinkled and with less number of hair follicles, sweat glands and sebaceous glands therefore, making it more susceptible to opportunistic pathogens. Superficial mycosis account for nearly 25% of the global burden of skin mycoses. The aim of this study is to identify the various superficial mycosis occurring in the elderly age group > 60 years attending a tertiary care hospital in North India. MATERIALS AND METHODS: Prospective, observational study conducted on 540 patients over the age of 60 years attending the Dermatology OPD at a tertiary care centre. Patients on antifungal agents were excluded from the study. Samples collected included skin and nail scrapings, nail clippings and hair along. The collected samples were divided into two parts one for culture and other for direct microscopy. Direct microscopy of the specimens was performed in 10% potassium hydroxide + 40% Di-methyl sulfoxide. Culture media used included Sabouraud’s dextrose agar (SDA) and Sabouraud’s dextrose agar containing Cycloheximide (0.5 mg/ml ). Cultures were incubated at both 25ºC and 37ºC for four weeks. Further species identification was done by Microslide culture technique.  RESULTS: The M:F ratio was 1.36:1. Onychomycosis accounted for the majority of superficial mycosis (25%) followed by Tinea mannum (14.65%) and Tinea pedis (15.51%). The most common dermatophyte isolate was Trichophyton rubrum (27.58%), followed by T.mentagrophytes (22.41%) and T.verrucosum (17.24%). KEY WORDS: Dermatophytes, Superficial Mycosis, Elderly age group, Slide culture

scholarly journals Speciation of candida species isolated in clinical samples in a tertiary health care centre in Northern India

2021 ◽  
Vol 7 (4) ◽  
pp. 262-268
Author(s):  
Pavneet Kaur ◽  
Tania Rawat ◽  
Sarabjeet Sharma ◽  
Parmeet Kaur
Keyword(s):  
Candida Species ◽  
Tertiary Care ◽  
Age Groups ◽  
Common Species ◽  
Clinical Samples ◽  
Care Hospital ◽  
Health Care Centre ◽  
Northern India ◽  
Sugar Fermentation ◽  
Culture Identification

The purpose of this study is to isolate, identify and specification of various the Candida species from various clinical samples in a tertiary care hospital, and to characterize various the isolated Candida species. A study was conducted on people of different age groups from January 2019 to December 2019. Candida species isolated from different patients by using Potassium Hydroxide mount and processed by BacTalert 3D (Biomerieux) automated blood culture system. Further culture identification of Candida species were done on Sabouraud dextrose agar (SDA). Speciation of Candida was done using Germ tube test, CHROM agar Candida Medium, Cornmeal agar, Sugar Fermentation test and Sugar Assimilation test.In our study was the most common species isolated, among non albicans Candida i.e. 21 (38.9%); 19(35.2%) of was the most common followed by 9(16.7%) ofand 5(9.3%) of . Maximum number of Candida isolates were obtained from NICU i.e. 27(50.0%) followed by 11 from Med (20.3%), 7 from E/W (13.0%), 2 from BICU (3.7%), 2 from Skin (3.7%), 1 from PICU (1.9%), and 1 from R/R (1.9%).Our study showed that is the most common isolates species. Among , was found to be the most common isolate followed by . Children less than 1 year are most affected with maximum number of Candida species were obtained from NICU department. HiChrom Candida is proven to be more useful as differential agar.

scholarly journals A Clinical-Bacteriological Profile of Multidrug Resistant Pseudomonas aeruginosa at Tertiary Care Hospital Shahjahanpur, Uttar Pradesh

2021 ◽  
Vol 10 (9) ◽  
pp. 301-313
Author(s):  
Jitendra Kumar Chaudhary
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Uttar Pradesh ◽  
Multidrug Resistant ◽  
Antimicrobial Treatment ◽  
Clinical Samples ◽  
Care Hospital ◽  
Bacteriological Profile ◽  
Drug Resistant Strains

The Pseudomonas aeruginosa is predominant agent causing nosocomial infections. In recent time, it develops resistance continuously to the antibiotics becomes Multidrug-resistant P. aeruginosa. So, in cystic fibrosis patents it difficult to eradicate P. aeruginosa infections with antimicrobial treatment. Therefore, focus on alternative mechanisms for treating P. aeruginosa infections. On the basis of growth, morphological and biochemical characteristics, P. aeruginosa strains were isolated from the clinical samples in this work. After that the antibiotic sensitivity was performed and the Multidrug resistant Pseudomonas aeruginosa was identified according to CLSI standard guideline chart by measuring the zone of inhibition for P. aeruginosa. The isolated strains showed resistance against three or more antibiotics, considered as MDR Pseudomonas aeruginosa. By antibiogram pattern 51 showed Multi drug resistant strains out of 102 isolated strains. As P. aeruginosa abide to develop resistance to the antibiotics, the quorum sensing increased transcriptional regulator QscR might performs another target. Thus the prevalence of MDR strains of Pseudomonas aeruginosa was investigated on current study. The antibiogram pattern revealed 51 MDR strains of P. aeruginosa. In which the majority of strains exhibited resistance towards Piperacillin (98%), Ciprofloxacin (90%), Ofloxacin (90%), Levofloxacin (80%) and Tobramycin (60%).

scholarly journals Spectrum of fungal infections at a tertiary care hospital in Haryana, India

2018 ◽  
Vol 6 (4) ◽  
pp. 1398
Author(s):  
Alfia Alim ◽  
Nidhi Goel ◽  
Uma Chaudhary
Keyword(s):  
Tertiary Care Hospital ◽  
Fungal Infections ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Care Hospital ◽  
Dematiaceous Fungi ◽  
Tertiary Care Centre ◽  
Female Ratio ◽  
Gram Staining ◽  
Hospitalised Patients

Background: In recent years, fungi have been emerging as a common infection in hospitalised patients of tertiary care centres. The climate of our country is well suited for a wide variety of fungal infections. Within the limited data available, an increased incidence of invasive candidiasis, aspergillosis and other dematiaceous fungi are reported at our tertiary care centre. This study was undertaken to know the prevalence of common fungal infections at a tertiary care hospital in a period of five years.Methods: The various clinical samples received from the patients presenting with clinically suspected fungal infections were subjected to direct microscopy with potassium hydroxide (KOH) and Gram staining. All samples were inoculated in duplicate SDA with or without antibiotics.Results: A total of 8450 suspected fungal etiology samples were received during the five years (2011-2015) included in the study. The most common sample was sputum 2502 (29.6%) followed by skin swabs 2175 (25.7%), urine 1302 (15.4%), blood 875 (10.35%) and BAL 810 (9.5%). Male to female ratio was 5:3. Candida albicans was the predominant isolate followed by non-albicans candida, Aspergillus flavus, Aspergillus fumigatus and dematiaceous fungi.Conclusions: The fungal isolates, which used to be discarded as laboratory contaminants are playing a significant role in pathogenicity of many infections. These organisms are now capable of affecting immunocompromised as well as immunocompetent individuals.

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