scholarly journals Effect of Interval and Continued Exercises with Crocin on Bax/Bcl-2 in Diabetic Obese Rats

2020 ◽  
Author(s):  
Ghobad Hassanpour ◽  
Hojatollah Nikbakht ◽  
Mohammad Ali Azarbayjani ◽  
Nader Shakeri ◽  
Hossein Abed Natanzi
Keyword(s):  
Comparison Method ◽  
Diabetic Rats ◽  
Treadmill Running ◽  
P Value ◽  
Significance Level ◽  
Interval Exercise ◽  
Low Intensity ◽  
Obese Rats ◽  
Kolmogorov Smirnov ◽  
Low Intensity Exercise

Objective: Diabetes is a metabolic disease which is linked to increased physical disabilities and muscle tissue damage. The aim of this study was to investigate the effect of interval and continued exercises with crocin on Bax/Bcl-2 ratio in diabetic obese rats. Materials and Methods: In this clinical trial study, 56 adult diabetic rats (high-fat diet and venous injection of streptozotocin) were selected and randomly assigned to groups (1), intense interval exercises (2), low intensity exercise (3), intense interval exercise with crocin consumption, (4) Low intensity exercise with crocin consumption, (5) Crocin consumption, (6) sham and (7) control were divided. Intense interval and low intensity exercise groups lasted for 8 weeks, three sessions per week, with intensity of 80 to 85 and 50 to 55 percent of maximum treadmill running, and crocin consumption groups for 8 weeks per day, mg / kg 25 crocin were taken peritoneal. To analyze the research hypotheses, Kolmogorov- Smirnov tests, independent T- tests and two-way multi-variable analysis of variance were used along with Benferron's comparison method. It should be noted that the significance level in all measurements was considered to be P -value≤ 0.05. Results: Results showed that exercise ( P -value: 0.12) and crocin ( P value: 0.10) consumption had no significant effect on Bax/Bcl-2 gene expression in diabetic rats. Also interaction of exercise and crocin consumption on Bax/Bcl-2 was not significant ( P -value: 0.12). Conclusion: It appears that exercise and crocin consumption have not interaction effect on improvement of Bax / Bcl-2 ratio in diabetic rats.

scholarly journals Acute Low-Intensity Treadmill Running Upregulates the Expression of Intestinal Glucose Transporters via GLP-2 in Mice

Nutrients ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1735
Author(s):  
Kai Aoki ◽  
Takuji Suzuki ◽  
Fang Hui ◽  
Takuro Nakano ◽  
Koki Yanazawa ◽  
...  
Keyword(s):  
Small Intestine ◽  
Intestinal Tract ◽  
Glucose Transporter ◽  
Treadmill Running ◽  
Low Intensity ◽  
Glucose Transporter 2 ◽  
Intestinal Digestion ◽  
Glucagon Like Peptide ◽  
Low Intensity Exercise ◽  
Carbohydrate Digestion

The effects of exercise on nutrient digestion and absorption in the intestinal tract are not well understood. A few studies have reported that exercise training increases the expression of molecules involved in carbohydrate digestion and absorption. Exercise was also shown to increase the blood concentration of glucagon-like peptide-2 (GLP-2), which regulates carbohydrate digestion and absorption in the small intestine. Therefore, we investigated the effects of exercise on the expression of molecules involved in intestinal digestion and absorption, including GLP-2. Six-week-old male mice were divided into a sedentary (SED) and low-intensity exercise (LEx) group. LEx mice were required to run on a treadmill (12.5 m/min, 1 h), whereas SED mice rested. All mice were euthanized 1 h after exercise or rest, and plasma, jejunum, ileum, and colon samples were collected, followed by analysis via IHC, EIA, and immunoblotting. The levels of plasma GLP-2 and the jejunum expression of the GLP-2 receptor, sucrase-isomaltase (SI), and glucose transporter 2 (GLUT2) were higher in LEx mice. Thus, we showed that acute low-intensity exercise affects the expression of molecules involved in intestinal carbohydrate digestion and absorption via GLP-2. Our results suggest that exercise might be beneficial for small intestine function in individuals with intestinal frailty.

scholarly journals HUBUNGAN PENGETAHUAN ORANG TUA TENTANG MAKANAN JAJANAN DENGAN KEJADIAN DIARE PADA ANAK SD NEGERI 1 BUAYAN KECAMATAN BUAYAN KABUPATEN KEBUMEN

2016 ◽  
Vol 12 (3) ◽  
Author(s):  
E Ernawati ◽  
Asih Maryani
Keyword(s):  
Sampling Technique ◽  
Parental Knowledge ◽  
Simple Random Sampling ◽  
Gastrointestinal Diseases ◽  
School Age Children ◽  
P Value ◽  
Street Food ◽  
Significance Level ◽  
Snack Foods ◽  
Kolmogorov Smirnov

Snack foods that are less qualified health and nutrition will threaten the health of school-age children as gastrointestinal diseases, one of which is diarrhea.This study aims to determine the relationship of parental knowledge about street food with the incidence of diarrhea in children SD Negeri 1 Buayan District of Kebumen.This study uses descriptive correlative with retrospective approach. The study population 192 respondents. The research sample 66 respondents. This study was conducted in February-March 2016. The samples were taken using simple random sampling technique. Analysis of data using the Kolmogorov-Smirnov correlation test with significance level of 0.05. Based on the research results, the majority of parental knowledge about good hawker food as much as 53 (80.3%) of respondents and elementary school children who are not suffering from diarrhea were 58 (87.9%) persons. The results of the analysis with the Kolmogorov-Smirnov can be known p-value of 0.008 with (p <0.05) so that there is a significant relationship between parental knowledge about street food with the incidence of diarrhea in children SD Negeri 1 Buayan District of Kebumen.There is a relationship between parental knowledge about street food with the incidence of diarrhea in children SD Negeri 1 Buayan District of Kebumen. Keywords: Diarrhea, Food Snacks, Knowledge

scholarly journals The Effect of Endurance Training Intensity on the Expression of Perlipin a Protein of Subcutaneous Adipose Tissue and Pancreatic B cells Function in Diabetic Rats

2020 ◽  
Author(s):  
Hadi Ghaedi
Keyword(s):  
B Cells ◽  
Endurance Training ◽  
High Intensity ◽  
Subcutaneous Adipose Tissue ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
P Value ◽  
Low Intensity ◽  
Pancreatic B Cells ◽  
Subcutaneous Adipose

Objective: The aim of this study was to compare the three endurance training intensities on the expression of Perlipin A protein in subcutaneous adipose tissue and pancreatic B-cells function in male diabetic rats. Materials and Methods: In this study, 40 healthy male wistar rats were divided into five groups, including diabetic with low intensity endurance training, diabetic with moderate intensity endurance training, diabetic with high intensity endurance training, diabetic control and healthy control. After diabetic induction with streptozotocin, endurance training was performed with low intensity, moderate and severe for eight weeks, three sessions per week. The relative expression of Perilipin A was measured by western blot technique. Results: The results indicated a significant effect of endurance training with three intensities on serum levels of insulin and glucose and pancreatic B-cells function ( P -value: 0.001). Also the results showed that there was no significant difference between Perlipin A expression in healthy and diabetic control groups with endurance training groups (with low, moderate and high intensity) ( P -value: 0.07). Conclusion: However, Moderate and high intensity endurance training compared to low-intensity training can compensate for the loss caused by diabetes in the expression of the Perlipin A protein but the difference was not significant. It seems that more intensity endurance training lead to more increase in Perlipin A expression in diabetic rats.

scholarly journals Efek Metode Latihan Drill dan Pendekatan Bermain melalui Koordinasi Mata Tangan untuk Meningkatkan Passing Bawah Volleyball di Sekolah Dasar [Effect of Drill Method and Play Approach through Hand Eye Coordination to Increase Passing Under Volleyball in El

2019 ◽  
Vol 8 (2) ◽  
pp. 157
Author(s):  
Defrizal Defrizal ◽  
Suharjana Suharjana
Keyword(s):  
Extracurricular Activities ◽  
P Value ◽  
Homogeneity Test ◽  
Training Methods ◽  
Test Results ◽  
Significance Level ◽  
Hand Eye Coordination ◽  
Kolmogorov Smirnov ◽  
Passing Ability ◽  
Women's Volleyball

This study aims to determine the effect of drill training methods and approaches to play with eye-hand coordination towards increasing under-passing volleyball. This study used . There was no comparison group in the design group. The population of this study was 40 female students who participated in volleyball extracurricular activities. Data analysis techniques used the Kolmogorov-Smirnov test for normality, homogeneity test and t-test to test hypotheses, samples correlated at a significance level of 5%. Test results show that there is a drill training effect on the ability to graduate. Hypothesis testing shows the value of t arithmetic (3.33)&gt; t table (2,080), and p value (0,003) &lt;0,05, indicating that Ho is rejected and Ha has been accepted means that there are significant differences in improving the ability to graduate after being treated. Based on the mean (mean) shows the average &gt; pretest. These results can be concluded that there is a significant effect of drill training on increasing the under-passing ability of women's volleyball in SD 1 .

Mixed lactate and caffeine compound increases satellite cell activity and anabolic signals for muscle hypertrophy

2015 ◽  
Vol 118 (6) ◽  
pp. 742-749 ◽  
Author(s):  
Yoshimi Oishi ◽  
Hayato Tsukamoto ◽  
Takumi Yokokawa ◽  
Keisuke Hirotsu ◽  
Mariko Shimazu ◽  
...  
Keyword(s):  
Satellite Cells ◽  
Cell Activity ◽  
Treadmill Running ◽  
C2c12 Cells ◽  
Protein Levels ◽  
Low Intensity ◽  
Proliferation And Differentiation ◽  
Total Dna ◽  
Low Intensity Exercise

We examined whether a mixed lactate and caffeine compound (LC) could effectively elicit proliferation and differentiation of satellite cells or activate anabolic signals in skeletal muscles. We cultured C2C12 cells with either lactate or LC for 6 h. We found that lactate significantly increased myogenin and follistatin protein levels and phosphorylation of P70S6K while decreasing the levels of myostatin relative to the control. LC significantly increased protein levels of Pax7, MyoD, and Ki67 in addition to myogenin, relative to control. LC also significantly increased follistatin expression relative to control and stimulated phosphorylation of mTOR and P70S6K. In an in vivo study, male F344/DuCrlCrlj rats were assigned to control (Sed, n = 10), exercise (Ex, n = 12), and LC supplementation (LCEx, n = 13) groups. LC was orally administered daily. The LCEx and Ex groups were exercised on a treadmill, running for 30 min at low intensity every other day for 4 wk. The LCEx group experienced a significant increase in the mass of the gastrocnemius (GA) and tibialis anterior (TA) relative to both the Sed and Ex groups. Furthermore, the LCEx group showed a significant increase in the total DNA content of TA compared with the Sed group. The LCEx group experienced a significant increase in myogenin and follistatin expression of GA relative to the Ex group. These results suggest that administration of LC can effectively increase muscle mass concomitant with elevated numbers of myonuclei, even with low-intensity exercise training, via activated satellite cells and anabolic signals.

Low-intensity exercise training delays onset of decompensated heart failure in spontaneously hypertensive heart failure rats

2005 ◽  
Vol 289 (5) ◽  
pp. H2030-H2038 ◽  
Author(s):  
Craig A. Emter ◽  
Sylvia A. McCune ◽  
Genevieve C. Sparagna ◽  
M. Judith Radin ◽  
Russell L. Moore
Keyword(s):  
Heart Failure ◽  
Exercise Training ◽  
Exercise Intervention ◽  
Decompensated Heart Failure ◽  
Treadmill Running ◽  
Width Ratio ◽  
Spontaneously Hypertensive ◽  
Low Intensity ◽  
Plasmic Reticulum ◽  
Low Intensity Exercise

Data regarding the effectiveness of chronic exercise training in improving survival in patients with congestive heart failure (CHF) are inconclusive. Therefore, we conducted a study to determine the effect of exercise training on survival in a well-defined animal model of heart failure (HF), using the lean male spontaneously hypertensive HF (SHHF) rat. In this model, animals typically present with decompensated, dilated HF between ∼18 and 23 mo of age. SHHF rats were assigned to sedentary or exercise-trained groups at 9 and 16 mo of age. Exercise training consisted of 6 mo of low-intensity treadmill running. Exercise training delayed the onset of overt HF and improved survival ( P < 0.01), independent of any effects on the hypertensive status of the rats. Training delayed the myosin heavy chain (MyHC) isoform shift from α- to β-MyHC that was seen in sedentary animals that developed HF. Exercise was associated with a concurrent increase in cardiomyocyte length (≈6%), width, and area and prevented the increase in the length-to-width ratio seen in sedentary animals in HF. The increases in proteinuria, plasma atrial natriuretic peptide, and serum leptin levels observed in rats with HF were suppressed by low-intensity exercise training. No significant alterations in sarco(endo)plasmic reticulum Ca2+ ATPase, phospholamban, or Na+/Ca2+ exchanger protein expression were found in response to training. Our results indicate that 6 mo of low-intensity exercise training delays the onset of decompensated HF and improves survival in the male SHHF rat. Similarly, exercise intervention prevented or suppressed alterations in several key variables that normally occur with the development of overt CHF. These data support the idea that exercise may be a useful and inexpensive intervention in the treatment of HF.

α2-AMPK activity is not essential for an increase in fatty acid oxidation during low-intensity exercise

2009 ◽  
Vol 296 (1) ◽  
pp. E47-E55 ◽  
Author(s):  
Shinji Miura ◽  
Yuko Kai ◽  
Yasutomi Kamei ◽  
Clinton R. Bruce ◽  
Naoto Kubota ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acid ◽  
Glucose Uptake ◽  
Fatty Acid Oxidation ◽  
Treadmill Exercise ◽  
Treadmill Running ◽  
Acid Oxidation ◽  
Low Intensity ◽  
Ampk Activity ◽  
Low Intensity Exercise

A single bout of exercise increases glucose uptake and fatty acid oxidation in skeletal muscle, with a corresponding activation of AMP-activated protein kinase (AMPK). While the exercise-induced increase in glucose uptake is partly due to activation of AMPK, it is unclear whether the increase of fatty acid oxidation is dependent on activation of AMPK. To examine this, transgenic mice were produced expressing a dominant-negative (DN) mutant of α1-AMPK (α1-AMPK-DN) in skeletal muscle and subjected to treadmill running. α1-AMPK-DN mice exhibited a 50% reduction in α1-AMPK activity and almost complete loss of α2-AMPK activity in skeletal muscle compared with wild-type littermates (WT). The fasting-induced decrease in respiratory quotient (RQ) ratio and reduced body weight were similar in both groups. In contrast with WT mice, α1-AMPK-DN mice could not perform high-intensity (30 m/min) treadmill exercise, although their response to low-intensity (10 m/min) treadmill exercise was not compromised. Changes in oxygen consumption and the RQ ratio during sedentary and low-intensity exercise were not different between α1-AMPK-DN and WT. Importantly, at low-intensity exercise, increased fatty acid oxidation in response to exercise in soleus (type I, slow twitch muscle) or extensor digitorum longus muscle (type II, fast twitch muscle) was not impaired in α1-AMPK-DN mice, indicating that α1-AMPK-DN mice utilize fatty acid in the same manner as WT mice during low-intensity exercise. These findings suggest that an increased α2-AMPK activity is not essential for increased skeletal muscle fatty acid oxidation during endurance exercise.

scholarly journals Acute Low-intensity Treadmill Running Induces Intestinal Glucose Transporters via GLP-2 in Mice

2021 ◽  
Author(s):  
Kai Aoki ◽  
Takuji Suzuki ◽  
Fang Hui ◽  
Takuro Nakano ◽  
Koki Yanazawa ◽  
...  
Keyword(s):  
Intestinal Tract ◽  
Glucose Transporter ◽  
Treadmill Running ◽  
Male Mice ◽  
Low Intensity ◽  
Intestinal Digestion ◽  
Glucagon Like Peptide ◽  
Small Intestinal ◽  
Low Intensity Exercise ◽  
Carbohydrate Digestion

Exercise affects various organs. However, its effects on nutrient digestion and absorption in the intestinal tract are not well understood. A few studies have reported that exercise training in-creases the expression of carbohydrate digestion and absorption molecules. Exercise was also shown to increase the concentration of blood glucagon like peptide-2(GLP-2), which regulates carbohydrate digestion and absorption in small intestinal epithelium. Therefore, we investigated the effects of exercise on intestinal digestion and absorption molecules and the levels of GLP-2. 6-wk-old of male mice were divided into 2 groups; sedentary (SED) and low-intensity exercise (LEx). LEx mice were required to run on a treadmill (12.5 m/min, 60 min), whereas SED mice rested. All mice were euthanized 1 h after exercise or rest and plasma, jejunum, ileum, and colon were sampled. Samples were analyzed using EIA and immunoblotting. The levels of plasma GLP-2 and the expression of the GLP-2 receptor, sucrase-isomaltase (SI), and glucose transporter (GLUT2) in the jejunum were increased in LEx group. We showed that acute low-intensity exer-cise affects the intestinal carbohydrate digestion and absorption molecules via GLP-2. Our results suggest that exercise might provide new benefits to the small intestine for people with intestinal frailty.

scholarly journals Low‐Intensity Exercise Training Prevents Muscular Microangiopathy in Non‐obese Type 2 Diabetic Rats

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Hidemi Fujino ◽  
Hiroyo Kondo ◽  
Sinichiro Murakami ◽  
Naoto Fujita ◽  
Takeshi Morifuji ◽  
...  
Keyword(s):  
Exercise Training ◽  
Diabetic Rats ◽  
Low Intensity ◽  
Low Intensity Exercise ◽  
Type 2 Diabetic
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