Evaluation of Antibacterial and Anti-Fungal Effect of Zinc Oxide Nano Particles to Complete Denture Tissue Conditioners

2021 ◽  
Author(s):  
Amin Khaleghi ◽  
Parisa Faghani ◽  
Mojtaba Azarian Borujeni
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Zinc Oxide ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Zno Nanoparticles ◽  
Bacterial Species ◽  
Nano Particles ◽  
Absolute Growth ◽  
Antibacterial And Antifungal

Introduction: The tissue conditioners are used to treat and prepare denture supporting tissue. This study aimedto evaluate the antibacterial and antifungal effect of zinc oxide nanoparticles on total denture tissue conditioners. Materials & Methods: In this laboratory study, 144 samples were collected and evaluated. Synthesized ZnO nanoparticles usingthe optic-optic method and, they were homogeneous with a mass fraction (0.625, 1.25, 2.5, 5, 10, and 20) according to the principles of the MIC method with tissue concentrators. In the present study, four bacterial species (Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Enterococcus faecalis), which represent different types of pathogen microorganisms, were used. For measuring the growth rate of microorganisms, a spectrophotometer was used based on turbidity with donereadings at 600 nm. Included tests were ANOVA and Tukey post hoc tests. Considered significant level of 0.05. Results: In the present study, increasing ZnO nanoparticles to tissue concentrators reduced the growth of all microorganisms studied. In the concentration of 20% absolute growth inhibition for Candida albicans, Streptococcus mutans, Enterococcus faecalis and Pseudomonas aeruginosa occurred. The best concentration for Candida, Enterococcus faecalis is in the concentration of 20% ZnO and, for Streptococcus mutans, Pseudomonas aeruginosa at a concentration of 5% ZnO. Conclusion: Increasing the ZnO nanoparticles to improve tissue improves the growth of microorganisms.

Evaluation of Antibacterial and Antifungal Properties of ZnO and Silver Nanoparticles against Candida Albicans and Streptococcus Mutans in Acrylic Resin

2021 ◽  
Author(s):  
Amin Khaleghi ◽  
Amin Archin ◽  
Mojtaba Azarian Borujeni
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Zno Nanoparticles ◽  
Acrylic Resin ◽  
Spherical Particles ◽  
Significance Level ◽  
Ultrasonic Apparatus ◽  
Experimental Laboratory ◽  
Antibacterial And Antifungal ◽  
Post Hoc

Introduction: This study aimed to evaluate the antibacterial and antifungal effect of Ag and zinc oxide nanoparticles to complete denture acrylic resin. Materials & Methods: In this experimental laboratory study, 144 samples were evaluated. The powder of acrylic resin (PMMA Ivoclar Vivadent, Lichenstein SR Triplex Hot) was mixed homogeneously in the ultrasonic apparatus with Ag (purity: 99.99%, size: 20nm, spherical) and ZnO (purity: 99%, size: 10-30 nm, nearly spherical) particles with mass fraction 0.5, 1 ,2 and 5. The microorganisms of this study were Candida albicans and Streptococcus mutans. The growth rate of microorganisms was measured by spectrophotometer based on turbidity and readings at 600 nm. Data were analyzed by ANOVA and Tukey post hoc tests. The significance level was set at 0.05. Results: Increasing Ag and ZnO nanoparticles to acrylic resin reduced the growth of Candida Albicans and Streptococcus Mutans. Even the concentration of 0.5% significantly reduced the growth of microorganisms. Complete growth inhibition was in the concentration of 5% after 24 and 48 hours. Conclusion: Increasing the Ag and ZnO nanoparticles to acrylic resin reduced the growth of microorganisms.

scholarly journals Antibacterial Activity of Zinc Oxide Nano Particles Against Bacteria Isolated from Infants with Urinary Tract Infection

2018 ◽  
Vol 29 (2) ◽  
pp. 34
Author(s):  
Azhar Jabbar Bohan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Zinc Oxide ◽  
Enterococcus Faecalis ◽  
Proteus Mirabilis ◽  
Zinc Oxide Nanoparticles ◽  
Nano Particles ◽  
Oxide Nanoparticles ◽  
Klebsiella Aerogenes ◽  
Healthy Children

This study included the collection of 70 sample of urine from infant aged between (2 days-2 years) divided into 35 sample of healthy children and 35 sample of children suffering from urinary tract infection, Through which get 40 isolated of different types of bacteria. And at different rates ranged between (27 , 12.8 , 7.1 , 5.7 , 4.2 % ) As percentages of the total isolation and (47.5 , 22.5 , 12.5 , 10 , 4.2 %) As percentages of isolation ratios qualitative for E.coli , Proteus mirabilis , Klebsiella Aerogenes, Pseudomonas aeruginosa and Enterococcus Faecalis respectively ,While the number of bacteria in sick children was ( 15, 8, 5, 3, 2 ) isolated for E.coli, Proteus mirabilis, Klebsiella Aerogenes, Pseudomonas aeruginosa and Enterococcus Faecalis respectively with isolation rates (42.85, 22.85, 14.28, 8.57, 5.7 %) Compared with healthy children who were (4) isolated for E.coli and with isolated ratios have (11.42%) and one isolated for each of Proteus mirabilis, Pseudomonas aeruginosa and Enterococcus Faecalis with isolated ratios (2.85 %) for this types respectively, also the preparation of zinc oxide nanoparticles done in Nanotechnology and Advanced Materials Research Center (NAMRC) in university of technology and Prepared of Zinc Oxide NanoParticles were investigated via using Scanning Electron Microscopy (SEM, the VEGA Easy Probe), X-ray powder diffraction (XRD) and antibacterial activity and the results of Zinc Oxide Nano Particles against bacteria explain a bility of it's to inhibition growth of bacteria in different rate of bacteriostatic when used in different concentration.

Acción antimicrobiana de dos pastas Guedes-Pinto modificadas - estudio in vitro

2021 ◽  
Vol 10 (1) ◽  
pp. 11
Author(s):  
Silvana Leal Vilas Bôas ◽  
Mariana Carvalho Furtado Leite ◽  
Samya Karolyne Barros Lavor Martins ◽  
Camila Costa Netto Muniz ◽  
Tamara Kerber Tedesco ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis

Objetivos: Evaluar in vitro la acción antimicrobiana de las pastas GuedesPinto modificadas con Diprogenta® y con Otosporin®, comparándolas con la pasta Guedes-Pinto convencional. Materiales y métodos: Se evaluó la acción antimicrobiana de las pastas Guedes Pinto contra cepas aisladas de los siguientes microbios: Streptococcus mutans ATCC 25175 Staphylococcus aureus ATCC 25923, Enterococcus faecalis - ATCC 29212, Pseudomonas aeruginosa - ATCC 4116, Candida albicans ATCC 10231. Se empleó la técnica de difusión en agar por el método de pocillo y en triplicado, utilizando el digluconato de clorexidina al 0,12% como control positivo y solución salina como control negativo. Se transfirió 2 mL del inóculo producido de cada microbio a 100mL de agar fundido a 45ºC, dispensando la mezcla en 30 placas de petri. Las placas fueron mantenidas a temperatura ambiente por dos horas y luego incubadas a 35ºC por 24 y 48 horas. La lectura de los resultados fue sometida al Análisis de varianza de un factor y Test de Tukey para comparaciones múltiples. Resultados: La pasta Guedes-Pinto Convencional formó los mayores halos de inhibición para la mayoría de los microbios, con excepción de Candida albicans, donde ninguna de las sustancias produjo efecto y de la Pseudomonas aeruginosa, para la cual la pasta modificada con Diprogenta® obtuvo mejores resultados, sin diferencia estadística entre ellas. La pasta Diprogenta® presentó mejores resultados que la pasta Otosporin® para todos los demás microorganismos y esto ultimo no formó halo de inhibición para Enterococcus faecalis. Conclusión: La utilización de sustitutos de Rifocort® parece tener un potencial antimicrobiano efectivo contra los principales microbios encontrados en los canales radiculares.

scholarly journals A Novel Developed Bioactive Composite Resin Containing Silver/Zinc Oxide (Ag/ZnO) Nanoparticles as an Antimicrobial Material against Streptococcus mutans, Lactobacillus, and Candida albicans

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Mojgan Kachoei ◽  
Baharak Divband ◽  
Mahdi Rahbar ◽  
Mahdiyeh Esmaeilzadeh ◽  
Milad Ghanizadeh ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Candida Albicans ◽  
Bond Strength ◽  
Streptococcus Mutans ◽  
Zno Nanoparticles ◽  
Shear Bond Strength ◽  
Composite Resin ◽  
Antimicrobial Properties ◽  
Composite Resins ◽  
The Mean

Aim. The objectives of this study were to develop a new bioactive composite resin containing silver/zinc oxide (Ag/ZnO) nanoparticles and investigate the effects on mechanical, cytotoxic, biocompatibility, and antimicrobial properties. Materials and Methods. Disc-shaped specimens were prepared from composite with and without nanoparticles in separate culture media containing Streptococcus mutans, Lactobacillus, and Candida albicans. Bracket bonding evaluation was performed on composite without nanoparticles (O), composite containing ZnO (Z) nanoparticles, composite containing ZnO nanoparticles and silver ions (A&Z), and composite containing Ag/ZnO nanoparticles (AZ) synthesized using optical precipitation. Results. Composite resin with nanoparticles (AZ, A&Z, and Z) showed significant antimicrobial properties ( P  < 0.05). The mean shear bond strength of A&Z composite resin (13.61 ± 0.73 MPa) was significantly less than that of conventional composite resin (19.03 ± 4.12 MPa) ( P  < 0.05). In addition, the mean shear bond strength of AZ composite resin (20.49 ± 1.03 MPa) was significantly higher than that of Z (16.35 ± 1.03 MPa) and A&Z composite resins. Conclusions. Incorporation of ZnO nanoparticles and their compounds into orthodontic composite resins induced antibacterial properties against oral pathogens, and of all these nanoparticles, the AZ group exhibited the best antimicrobial activity and highest shear bond strength.

scholarly journals Influência da adição de piperina na ação antimicrobiana do Óxido de Zinco e Eugenol (OZE): OZE/PIPERINA uma nova proposta de cimento odontológico

2021 ◽  
Vol 10 (10) ◽  
pp. e330101018804
Author(s):  
Humbérila da Costa e Silva Melo ◽  
José Luiz Silva Sá ◽  
Francisca Lúcia de Lima ◽  
Robson Almeida Borges de Freitas ◽  
Iron Jonhson de Araújo Veras ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
E Coli

A Piperina é a principal amida alcaloide extraída das sementes de Piper ningrum L., apresenta propriedades biológicas do tipo: antimicrobiana, anti-inflamatória, biodisponibilidade de fármacos. Óxido de Zinco-Eugenol (OZE) juntos, constituem os cimentos amplamente utilizados na odontologia como curativo temporário. Esse estudo teve como objetivo verificar a ação antimicrobiana da composição a partir de Piperina (PIP) e OZE. Utilizou-se o método de difusão em ágar com perfuração de poços, em soluções OZE/PIP com diferentes porcentagens de piperina: 4, 5, 10 e 20%.  As cepas de referências foram Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis e Candida albicans. A concentração de piperina 4% apresentou os maiores halos de inibição quando testadas com as cepas P. aeruginosa (29mm), S. aureus (26mm), E. coli (24mm). O aumento da concentração de piperina na composição não influenciou os tamanhos dos halos de inibição, exceto o S. mutans que formou halo de 15 mm na composição com 5% de piperina e Enterococcus que formou halos de 14 mm com 5 e 20% de piperina. A composição mostrou-se eficiente, em suas diferentes proporções, no combate a microrganismos mais resistentes (S. aureus), e nos microrganismos mais comuns na cavidade oral (S. mutans, C. albicans).

scholarly journals Antibacterial and antifungal activity of endodontic intracanal medications

2017 ◽  
Vol 90 (3) ◽  
pp. 344-347 ◽  
Author(s):  
Andrada Tonea ◽  
Mandra Badea ◽  
Liviu Oana ◽  
Sorina Sava ◽  
Dan Vodnar
Keyword(s):  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Bacterial Species ◽  
Aloe Vera ◽  
Experimental Plant ◽  
Aloe Barbadensis ◽  
Arctium Lappa ◽  
Antifungal Properties ◽  
Antibacterial And Antifungal

Background and aims. The sterilization of the entire root canal system represents the main goal of every endodontist, given the fact that the control of the microbial flora is the key point of every root canal treatment. The diversity of microorganisms found inside the root canal and also the resistance of some bacterial species to intracanal medications led to a continuous development of new endodontic products. The present study focuses on the comparison of the antibacterial and antifungal properties of different endodontic products, two commercially available, one experimental plant based extract, and two control substances.Methods. The disc diffusion assay was used to determine the antibacterial and antifungal properties of chlorhexidine, calcium hydroxide, a mix extract between Arctium lappa root powder and Aloe barbadensis Miller gel, Amoxicillin with clavulanic acid and Fluconazole (as control substances). Two of the most common microorganisms found in endodontic infections were chosen: Enterococcus faecalis (ATCC 29212) and Candida albicans ATCC(10231).Results. All tested substances showed inhibition zones around the discs, for Enterococcus faecalis and Candida albicans, including the experimental mix extract of Arctium lappa root powder with Aloe vera gel.Conclusion. The experimental mix extract of Arctium lappa root powder and Aloe vera gel is able to inhibit very resistant microorganisms, like Enterococcus faecalis and Candida albicans.  

scholarly journals Componentes químicos y actividad antimicrobiana del aceite esencial de Lasiocephalus ovatus (Asteraceae) que crece en Ecuador

2020 ◽  
Vol 25 (1) ◽  
pp. 22-28
Author(s):  
Liliana Margarita Araujo Baptista ◽  
Katerine Vimos-Sisa ◽  
Rosa Cruz-Tenempaguay ◽  
Félix Falconí-Ontaneda ◽  
Luis Rojas-Fermín ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis

Ecuador es uno de los países más ricos en biodiversidad y endemismo del mundo, y cerca de 3200 especies de plantas tienen usos medicinales. El objetivo de esta investigación fue evaluar la composición química y el efecto antimicrobiano del aceite esencial de Lasiocephalus ovatus Schltdl. (Asteraceae) colectada en la provincia de Chimborazo, Ecuador. Las partes aéreas de L. ovatus fueron sometidas a hidrodestilación para obtener el aceite esencial, el cual fue analizado mediante cromatografía de gases acoplada a espectrometría de masas. La actividad fue evaluada frente a cinco bacterias y una levadura usando la técnica de microdilución en caldo en microplacas de 96 pozos. El rendimiento del aceite fue de 0,05 % y 27 compuestos fueron identificados, representando 95,45 % de la composición total con un elevado contenido de monoterpenos oxigenados (52,17 %). Los compuestos mayoritarios fueron alcanfor (40,48 %) y 1,2,5,5-tetrametil-1,3-ciclopentadieno (11,90 %), seguido por p-menta-1,5-dien-8-ol (5,23 %) y 1,6-dimetilhepta-1,3,5-trieno (4,69 %). Las bacterias más sensibles a la acción del aceite fueron Staphylococcus aureus ATCC 25923 y Escherichia coli ATCC 25922 con concentraciones mínimas inhibitorias de 200-400 μg/mL y bactericidas de 800 μg/mL. La inhibición antimicrobiana frente a las bacterias Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603 y la levadura Candida albicans ATCC 10231 fue baja, con un rango de concentración mínima inhibitoria de 800 a 6400 μg/mL. Este reporte representa un primer análisis de la actividad antimicrobiana del aceite de L. ovatus, por lo tanto, una contribución importante al estudio del género Lasiocephalus.

scholarly journals Molecular Determinants of the Thickened Matrix in a Dual-Species Pseudomonas aeruginosa and Enterococcus faecalis Biofilm

2017 ◽  
Vol 83 (21) ◽  
Author(s):  
Keehoon Lee ◽  
Kang-Mu Lee ◽  
Donggeun Kim ◽  
Sang Sun Yoon
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Synergistic Effects ◽  
Bacterial Species ◽  
Extracellular Dna ◽  
Infection Model ◽  
Surgical Removal ◽  
Chronic Infections ◽  
Content Type ◽  
Biofilm Matrix

ABSTRACT Biofilms are microbial communities that inhabit various surfaces and are surrounded by extracellular matrices (ECMs). Clinical microbiologists have shown that the majority of chronic infections are caused by biofilms, following the introduction of the first biofilm infection model by J. W. Costerton and colleagues (J. Lam, R. Chan, K. Lam, and J. W. Costerton, Infect Immun 28:546–556, 1980). However, treatments for chronic biofilm infections are still limited to surgical removal of the infected sites. Pseudomonas aeruginosa and Enterococcus faecalis are two frequently identified bacterial species in biofilm infections; nevertheless, the interactions between these two species, especially during biofilm growth, are not clearly understood. In this study, we observed phenotypic changes in a dual-species biofilm of P. aeruginosa and E. faecalis, including a dramatic increase in biofilm matrix thickness. For clear elucidation of the spatial distribution of the dual-species biofilm, P. aeruginosa and E. faecalis were labeled with red and green fluorescence, respectively. E. faecalis was located at the lower part of the dual-species biofilm, while P. aeruginosa developed a structured biofilm on the upper part. Mutants with altered exopolysaccharide (EPS) productions were constructed in order to determine the molecular basis for the synergistic effect of the dual-species biofilm. Increased biofilm matrix thickness was associated with EPSs, not extracellular DNA. In particular, Pel and Psl contributed to interspecies and intraspecies interactions, respectively, in the dual-species P. aeruginosa and E. faecalis biofilm. Accordingly, targeting Pel and Psl might be an effective part of eradicating P. aeruginosa polymicrobial biofilms. IMPORTANCE Chronic infection is a serious problem in the medical field. Scientists have observed that chronic infections are closely associated with biofilms, and the vast majority of infection-causing biofilms are polymicrobial. Many studies have reported that microbes in polymicrobial biofilms interact with each other and that the bacterial interactions result in elevated virulence, in terms of factors, such as infectivity and antibiotic resistance. Pseudomonas aeruginosa and Enterococcus faecalis are frequently isolated pathogens in chronic biofilm infections. Nevertheless, while both bacteria are known to be agents of numerous nosocomial infections and can cause serious diseases, interactions between the bacteria in biofilms have rarely been examined. In this investigation, we aimed to characterize P. aeruginosa and E. faecalis dual-species biofilms and to determine the molecular factors that cause synergistic effects, especially on the matrix thickening of the biofilm. We suspect that our findings will contribute to the development of more efficient methods for eradicating polymicrobial biofilm infections.

scholarly journals Pseudomonas aeruginosa secreted factors impair biofilm development in Candida albicans

Microbiology ◽  
2010 ◽  
Vol 156 (5) ◽  
pp. 1476-1486 ◽  
Author(s):  
Lucy J. Holcombe ◽  
Gordon McAlester ◽  
Carol A. Munro ◽  
Brice Enjalbert ◽  
Alistair J. P. Brown ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Bacterial Species ◽  
Homoserine Lactone ◽  
Strong Increase ◽  
Opportunistic Pathogens ◽  
Secreted Factors ◽  
Expression Of Genes ◽  
Biofilm Development

Signal-mediated interactions between the human opportunistic pathogens Pseudomonas aeruginosa and Candida albicans affect virulence traits in both organisms. Phenotypic studies revealed that bacterial supernatant from four P. aeruginosa strains strongly reduced the ability of C. albicans to form biofilms on silicone. This was largely a consequence of inhibition of biofilm maturation, a phenomenon also observed with supernatant prepared from non-clinical bacterial species. The effects of supernatant on biofilm formation were not mediated via interference with the yeast–hyphal morphological switch and occurred regardless of the level of homoserine lactone (HSL) produced, indicating that the effect is HSL-independent. A transcriptome analysis to dissect the effects of the P. aeruginosa supernatants on gene expression in the early stages of C. albicans biofilm formation identified 238 genes that exhibited reproducible changes in expression in response to all four supernatants. In particular, there was a strong increase in the expression of genes related to drug or toxin efflux and a decrease in expression of genes associated with adhesion and biofilm formation. Furthermore, expression of YWP1, which encodes a protein known to inhibit biofilm formation, was significantly increased. Biofilm formation is a key aspect of C. albicans infections, therefore the capacity of P. aeruginosa to antagonize this has clear biomedical implications.

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