The Effect of Biochanin A as PPAR γ agonist on LDL Particles Diameter and Type 2 Diabetic Dyslipidemia

2019 ◽  
Author(s):  
Darya Ghadimi ◽  
Mohammad Taghi Taghi Goodarzi ◽  
Mahdi Bahmani ◽  
Zohre Khajehahmadi
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Male Rats ◽  
Biochanin A ◽  
Diabetic Patients ◽  
Low Density ◽  
Diabetic Dyslipidemia ◽  
Ldl Particles

Background and Aims: Small dense  low-density lipoproteins (sd-LDL) particles are smaller and heavier than typical LDL ones. They can penetrate into the endothelium of coronary arteries more easily because of their small size. Diabetes mellitus is accompanied by dyslipidemia such as increasing concentration of plasma very low density lipoprotein and sd-LDL. Peroxisome proliferator activated receptor γ (PPARγ ) can decrease the level of sd-LDL in plasma. Biochanin A (BCA), a natural compound, is a PPARγ agonist. The present study was designed to investigate the effect of BCA on sd-LDL-Clolesterol level in diabetic animals. Materials and Methods: Adult male rats (Wistar strain) were used as the animal models in this study. Animals were made diabetic by single intraperitoneal injection of Streptozotocin- Nicotinamide and then treated by 1 and 5 mg/kg of BCA for 28 days. Body weight and fasting blood glucose were also tested before and at the end of treatment. Furthermore, the size of LDL particles were measured by nondenaturing polyacrylamide gradient gel electrophoresis assay. Results: Results of the present study indicated that BCA administration at dose of 5mg/kg decreased fasting blood glucose level and increased body weight and diameter of LDL particles in diabetic animals significantly. Conclusions: BCA seems to be an appropriate agent in diabetes mellitus, because it improves the diabetic dyslipidemia, which is the most important complication in diabetic patients.

Controlling Hyperglycaemia Diabetes Mellitus Type-II: Bromocriptine Alone or in Combination with Metformin

2021 ◽  
Vol 15 (12) ◽  
pp. 3348-3350
Author(s):  
Khadija Mastoor ◽  
Faisal Idrees Khan ◽  
Iram Imran ◽  
Anila Errum ◽  
Sadia Sharif ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Pancreatic Beta Cells ◽  
Male Rats ◽  
Diabetic Patients ◽  
Group 2 ◽  
Therapeutic Doses

Background: Diabetes Mellitus is one of the leading cause of morbidity and mortality globally. According to WHO there is a steady increase in the number of diabetic patients annually. Maintenance of good glycaemic control in type 2 diabetic patients typically becomes progressively more difficult as the duration of disease lengthens due to decline in the capacity of the pancreatic beta cells for glucose stimulated insulin release, in the presence of insulin resistance. This study is conducted to observe the effect of metformin and bromocriptine individually and sub-therapeutic doses of these drugs when used as a combination therapy. Objective: The objective of this study is to primarily investigate and then compare the antihyperglycemic effects of bromocriptine with metformin, also to see the combined effect of sub therapeutic doses of both these drugs. Methodology: Random allotment of 24 albino male rats was done in four groups. Group 1 was kept as control. Alloxan monohydrate was given to group 2, 3 and 4 and diabetes was induced. Group 2 and 3 were treated with metformin (1.5mg/kg body weight) and bromocriptine (3 mg/kg body weight) respectively while group 4 was treated with sub therapeutic doses of metformin (1 mg/kg body weight) and bromocriptine (1.5 mg/kg body weight)both. Serum glucose levels were estimated at 1, 10, 20 and 30 days. Results: Results showed that metformin reduces blood glucose level significantly where as bromocriptine also showed reduction of blood glucose level but not as significantly as metformin. However, the combination of metformin and bromocriptine showed much reduction in blood glucose level than metformin and bromocriptine used alone. Conclusion: Bromocriptine and metformin when combined ameliorated blood guclose efficiently than given alone Keywords: Diabetes mellitus, bromocriptine, metformin

scholarly journals Comparative Analysis of the Hypoglycaemic and Hypolipidemic Effects of Aqueous Extract of some Ethno medicinal Plants in Alloxan Induced Diabetic Rats

2021 ◽  
pp. 40-48
Author(s):  
Idris Aliyu Kankara ◽  
Akos Noel Ibrahim ◽  
Haruna Ade
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Albino Rats ◽  
Low Density ◽  
Fasting Blood Glucose Level

Diabetes mellitus is a complex metabolic disorder associated with the development of metabolic complications. This research evaluated the hypoglycemic and hypolipidaemic effects of Moringa oleifera (MO), Treculia africana (TA) and Albizzia chevalieri (AC) plant extracts on diabetes. Albino rats were randomly divided into six (six) main groups; MC, MO, TA, AC, Normal Control (NC) and Diabetic Control (DC) groups. Group MO, TA and AC were further subdivide into three sub groups. Diabetes mellitus was induced by a single dose intraperitoneal injection of alloxan 150 mg/kg body weight. Fasting blood glucose level and lipid profile were assayed using standard methods. Intraperitoneal injection of 150 mg/kg of Alloxan in the albino rats resulted in significant (p<0.05) elevation of serum glucose, total cholesterol (TC), triglyceride (TG), very low density lipoprotein (VLDL-C), and low density lipoprotein (LDL-C). Also, there was significant decrease (p<0.05) in HDL-C and body weight of the albino rats compared with that of the NC group. Oral administration of MO, TA and AC to diabetic albino rats for 21 days significantly (p<0.05) reduced fasting blood glucose level, normalized lipid profile and restore body weight of the albino rats in treated groups compared to diabetic control groups. All the plant extracts studied in this research significantly (p<0.05) increase the regeneration of damaged pancreatic β cells. Treatment with MO (800 mg/kg) confirmed highly significant (p<0.05) effect compared to TA and AC.

scholarly journals Evaluation of type-2 diabetes mellitus patients for dyslipidemia in a tertiary care centre

2020 ◽  
Vol 7 (4) ◽  
pp. 586
Author(s):  
Janak G. Chokshi ◽  
Apal P. Gandhi ◽  
Ishvarlal M. Parmar ◽  
Dipen R. Damor
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Plasma Glucose ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Patients ◽  
Low Density ◽  
Increased Risk

Background: Diabetes mellitus (DM) is a syndrome consisting of metabolic, vascular and neuropathic components that are interrelated. Diabetes mellitus is associated with a considerably increased risk of premature atherosclerosis, particularly coronary heart disease (CHD) and peripheral arterial disease. Dyslipidemia is a common feature of diabetes. There is an association between atherosclerotic cardiovascular disease and serum cholesterol and triglyceride levels in both type 1 and type 2 diabetes.Methods: The study was done on 50 adult diabetes mellitus (T2) patients from IPD of General Medicine wards at SMS Hospital, Ahmedabad, Gujarat. 50 healthy age and sex matched healthy volunteers were taken as control. They were evaluated for lipid profile i.e., Total Cholesterol (TC),Triglyceride (TG), Low-density lipoprotein (LDL), High density lipoprotein (HDL), Very low density lipoprotein (VLDL) and glycemic status i.e., Fasting blood glucose (FBS), Postprandial 2 hours blood glucose (PP2BS) & Glycosylated haemoglobin(HbA1C).Results: Diabetic cases had statistically highly significant (p<0.001) elevated levels of total Cholesterol, Triglycerides and VLDL as compared to controls. Serum TG, serum TC, LDL-C and VLDL-C had positive correlation with the postprandial plasma glucose, fasting plasma glucose and HbA1c.Conclusions: Significant correlations between HbA1c levels and lipid levels point towards the usefulness of HbA1c for screening high-risk diabetic patients. High TC, TG, LDL-C and HbA1c with normal or low HDL-C is seen in almost all diabetic patients either alone or in combinations.

scholarly journals Alterations of liver enzymes in T2DM: a case control study

2018 ◽  
Vol 5 (6) ◽  
pp. 1521
Author(s):  
Chandrashekhar G. S.
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Case Control Study ◽  
Liver Enzymes ◽  
Fasting Blood Glucose ◽  
Case Control ◽  
Diabetic Patients ◽  
Histopathological Analysis ◽  
Control Study

Background: Liver plays an important role in regulation of blood glucose in fed state as well as in fasting. Diabetes mellitus can result as a consequence of liver disorder and vice versa. Objective of the present study is to compare the liver enzymes in type 2 diabetic patients as compared to non-diabetic patients.Methods: A case- control study was conducted in Clinical Biochemistry Laboratory, Adarsha Super speciality Hospital, Udupi from April 2018 to August 2018. The data of 174 diabetic patients and 118 healthy people as controls was collected. Fasting blood glucose, aspartate aminotransferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) were estimated in the study subjects.Results: It was found that AST levels (47.55±4.69U/L) in diabetics extremely significantly high as compared to controls (33.51±2.33U/L). ALT levels were insignificantly high in diabetics compared to controls. ALP was significantly elevated (p=0.0002) in diabetics. Correlation study showed a weak positive correlation between AST, ALT and blood glucose. Odds ratio showed a higher risk of liver enzyme elevation in diabetics. Risk of elevation of AST was found to be 1.65 times high and ALT was 1.25 times high in diabetics compared to non-diabetics.Conclusions: Diabetics had high liver enzymes as compared to non-diabetics. An association was found between type 2 diabetes mellitus and liver enzymes. For better characterization of cause and effect, further studies need to be done on alterations in liver function tests along with the histopathological analysis of liver biopsy samples.

scholarly journals Effect of Peanut (Arachis hypogaea L.) on Fasting Blood Glucose and HbA1c in Alloxan Induced Diabetic Male Rats

2015 ◽  
Vol 9 (2) ◽  
pp. 48-53
Author(s):  
Fatema Akter ◽  
Nasim Jahan ◽  
Nayma Sultana
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Arachis Hypogaea ◽  
Fasting Blood Glucose ◽  
Basal Diet ◽  
Male Rats ◽  
Arachis Hypogaea L ◽  
Anti Oxidant ◽  
Hba1c Levels

Background: Diabetes mellitus (DM) is a heterogeneous group of metabolic disorders with micro and macrovascular complications which are the major causes of morbidity and mortality in diabetic patients. Peanut due to its anti-oxidant property can reduce blood glucose level and may reduce the risk of diabetes. Objective: To observe the anti-diabetic effects of peanut (Arachis hypogaea L.) in alloxaninduced diabetic male rats. Method: This experimental study was carried out in the Department of Physiology, Sir Salimullah Medical College (SSMC), Dhaka in 2013. For this purpose, 15 wistar albino male rats, aged 90-120 days, weighing 160-200 g (initial body weight) were included in the experimental group (DG-A-P). Age and weight matched 20 wistar albino rats without peanut supplementation was taken as control and divided into (BC and DC-A) . All groups of animals received basal diet for 21 consecutive days. In addition to basal diet, animals of DC-A received alloxan intraperitoneally only on the first day of the study. Moreover, animals of DG-A-P also received peanut extract for 21 consecutive days. After 16 hours of fasting blood samples were collected from tail vein of all rats on day-1 and day- 3. After taking final body weight all the rats were sacrificed on 22nd day. Their pancreas was removed and weighed. To observe glycemic control serum blood glucose and blood HbA1c levels were measured by usual laboratory technique. ANOVA, independent t-test and paired t-test were used for statistical analysis. Result: The % change of body weight was significantly (p<0.001) lower in DC-A in comparison to that of BC. The weight of pancreas was significantly lower in DC-A (p<0.001) and DG-A-P (p<0.05) when compared to that BC, whereas this level was significantly higher (p<0.001) in DG-A-P than that of DC-A. The mean fasting blood glucose level on day-3 was significantly (p<0.001) higher in DC-A and DG-A-P in comparison to that of BC. Then this level was significantly (p<0.001) lower in DG-A-P in comparison to that of DC-A on day-22. Moreover, significantly higher levels of HbA1c were observed in DC-A (p<0.001) and DG-A-P (p<0.05) in comparison to those of BC. Again, significantly (p<0.001) lower level of blood HbA1c were observed in DG-A-P than those of DC-A. Conclusion: From this study, it can be concluded that peanut (Arachis hypogaea L.) has anti-diabetic effect by reducing fasting blood glucose, HbA1c levels. This anti-diabetic effect may be due to its high MUFA content and anti-oxidant property. DOI: http://dx.doi.org/10.3329/jbsp.v9i2.22796 Bangladesh Soc Physiol. 2014, December; 9(2): 48-53

scholarly journals Evaluation of the hypolipidemic effect of Gangaria de flush herbal mixture in streptozotocin-induced diabetic male rats

2021 ◽  
Vol 11 (3) ◽  
pp. 066-071
Author(s):  
Babafemi Tosin Ogunbiyi ◽  
Gogonte Hezekiah Amah ◽  
Laide Omoyiola Olajide ◽  
Olubunmi Esther Ogunbiyi ◽  
Oluwafunmilola Christiana Adetunji ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Hypolipidemic Effect ◽  
Low Density ◽  
Herbal Mixture ◽  
Chronic Hyperglycemia

Diabetes mellitus is a complex multifarious metabolic disorder characterized by chronic hyperglycemia that requires non-stop treatment with multiple factors that will reduce the risk of long-term complications and drug side effects. Streptozotocin is well known for its selective pancreatic islet β-cell cytotoxicity used to induce diabetes mellitus in animals. It also interferes with cellular metabolic oxidative mechanisms. Phytomedicine has been in existence since the primitive age and has proven to be effective in treating this condition. This study was aimed at evaluating the effect of Gangaria de flush (Gdf) herbal mixture on lipid profile in streptozotocin (STZ) (60mg/kg) induced diabetic rat. The diabetic rats were treated with Gangaria de flush (0.3ml/kg body weight) and Glibenclamide (5mg/kg body weight) for 7days. The effects of both Gangaria de flush herbal mixture and glibenclamide were analyzed. Gangaria de flush showed a reduction in the lipid profile with a significant percentage reduction of 45.9% for the total cholesterol, 65.7% for the total triglyceride, 63.5% for the low-density lipoprotein, 65.8% for the very low-density lipoprotein as against the negative control and a 7.6% significant increase in the high density lipoprotein. Gangaria de flush showed a significant (p<0.05) positive effect on the lipid profile. Hence, it can be suggested that Gangaria de flush herbal mixture has a significant effect in maintaining the lipid level.

scholarly journals ANTI-DIABETIC EFFECT OF ETHANOL EXTRACT OF Copaifera salikounda (HECKEL) AGAINST ALLOXAN-INDUCED DIABETES IN RATS

2021 ◽  
Vol 58 (2) ◽  
Author(s):  
Chinyere Aloke ◽  
Emmanuel Igwe ◽  
Nwogo Obasi ◽  
Pascal Amu ◽  
Egwu Ogbonnia
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Medicinal Plants ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
The Body ◽  
Albino Rats ◽  
Dependent Manner

Accumulating evidences have reinforced the use of medicinal plants in the treatment of various ailments as a result of negative side effects associated with conventional drugs. Plant components such as phenols and flavonoids with antioxidant potential have confirmed protective roles against oxidative stress-induced degenerative diseases like diabetes mellitus (DM). The current study was carried out to investigate the effect of seed pod ethanol extract from Copaifera salikounda (SPEECS) in alloxan-induced diabetic rats. SPEECS was obtained by maceration of seed pod powder in absolute ethanol for 72 h, filtered, concentrated and dried in-vacuo. Gas chromatography-mass spectrometry (GC–MS) technique was used to quantitatively elucidate the chemical constituents of SPEECS. Twenty-four male albino rats were randomly allocated into four groups (n=6): normal control, DM control, DM + 200 mg/kg SPEECS and DM + 400 mg/kg SPEECS groups. DM was induced in the Wistar albino rats through intraperitoneal injection of 200 mg/kg body weight of alloxan. After 14 days of treatment, the body weight changes and the fasting blood glucose level were determined in the different groups. Also, serum biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), albumin (ALB), total protein (TP), malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were estimated. The GC-MS results confirm nine bioactive compounds with 9-octadecenoic acid (55.75%) being most abundant. SPEECS (200 and 400 mg/kg) administration significantly (P 0.05) caused gain in weight, decreased fasting blood glucose and reversed the elevated liver function enzymes (ALT, AST, ALP) while total TP and ALB were markedly elevated relative to DM control group. Furthermore, SPEECS attenuated the activities of SOD and CAT while the level of MDA was significantly (P 0.05) decreased in dose dependent manner in comparison to the DM control. This study indicated that SPEECS can alleviate hyperglyceamia of DM. Key words: Copaifera salikounda; oxidative stress; medicinal plants; diabetes mellitus; phytochemicals; orthodox ANTIDIABETIČNI UČINEK EKSTRAKTA ETANOLA Copaifera salikounda (HECKEL) NA SLADKORNO BOLEZEN, SPROŽENO Z ALLOXAN-om, PRI PODGANAHIzvleček: Obstaja vedno več dokazov, ki poudarjajo uporabnost zdravilnih rastlin pri zdravljenju različnih bolezni, tudi zaradi različnih negativnih stranskih učinkov, povezanih s konvencionalnimi zdravili. Rastlinske sestavine kot so fenoli in flavonoidi z antioksidativnim potencialom, imajo po nekaterih raziskavah zaščitno vlogo pred degenerativnimi boleznimi, ki jih povzroča oksidativni stres, kot je sladkorna bolezen diabetes mellitus (DM). Študija je bila izvedena z namenom raziskovanja učinka etanolnega semenskega ekstrakta iz rastline Copaifera salikounda (SPEECS) pri podganah s sladkorno boleznijo, ki jo je povzročil alloxan. SPEECS je bil pridobljen z maceracijo praška semen v prahu v absolutnem etanolu 72 ur ter nadaljnjo filtracijo, koncentracijo in sušenjem v vakuumu. Za kvantitativno ugotavljanje kemijskih sestavin SPEECS je bila uporabljena tehnika plinske kromatografije in masne spektrometrije (GC-MS). Štiriindvajset samcev podgan Wistar je bilo naključno razporejenih v štiri skupine (n=6): normalna kontrola, kontrola DM, DM + 200 mg/kg SPEECS in DM + 400 mg/kg SPEECS. DM je bil pri podganah sprožen z intraperitonealno injekcijo 200 mg/kg telesne mase alloxana. Po 14 dneh zdravljenja so bile pri različnih skupinah določene spremembe telesne teže in nivo glukoze v krvi (na tešče). Poleg tega so avtorji raziskave izmerili še nekatere serumske biokemične parametre kot so ravni alaninske aminotransferaze (ALT), aspartatne aminotransferaze (AST), alkalne fosfataze (ALP), albumina (ALB), skupnih proteinov (TP), malondialdehida (MDA), superoksiddismutaze (SOD) in katalaze (CAT). Rezultati GC-MS so v izvlečku SPEECS pokazali devet bioaktivnih spojin, v katerih je največ 9-oktadecenojske kisline (55,75%). SPEECS (200 in 400 mg/kg) je povzročil znatno (P 0,05) povečanje telesne mase, znižanje glukoze v krvi na tešče in znižal raven encimov pokazateljev jetrne funkcije (ALT, AST, ALP), medtem ko je bila raven TP in ALB pri podganah, ki so prejemale SPEECS izrazito povišana v primerjavi z DM kontrolno skupino. Zdravljenje s  SPEECS je tudi oslabilo aktivnosti SOD in CAT, medtem ko se je raven MDA znatno zmanjšala (P 0,05) v primerjavi s kontrolno skupino DM. Ta študija je pokazala, da lahko SPEECS ublaži hiperglikemijo pri sladkorni bolezni pri podganah.Ključne besede: Copaifera salikounda; oksidativni stres; zdravilne rastline; sladkorna bolezen; fitokemikalije; ortodoksni

scholarly journals Metformin reduces circulating malondialdehyde-modified low-density lipoprotein in type 2 diabetes mellitus

2014 ◽  
Vol 37 (4) ◽  
pp. 243 ◽  
Author(s):  
Masahiro Ohira ◽  
Takashi Yamaguchi ◽  
Atsuhito Saiki ◽  
Noriko Ban ◽  
Hidetoshi Kawana ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Blood Glucose ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Hypoglycemic Agents ◽  
Low Density

Purpose: Type 2 diabetes is known to be associated with increasing cardiovascular mortality. Malondialdehyde-modified LDL (MDA-LDL) is an oxidized LDL and is increased in patients with diabetes or hypertriglyceridemia. Elevated MDA-LDL has been reported to be a risk factor of atherosclerosis or cardiovascular disease. Sitagliptin is a dipeptidyl peptidase-4 inhibitor and a new class of hypoglycemic agents. In this study, the effects of increasing the dose of metformin and add-on sitagliptin on MDA-LDL were examined in type 2 diabetes patients. Methods: Seventy patients with type 2 diabetes, inadequately controlled despite on-going treatment with metformin 500 mg/day, were enrolled in this randomized controlled trial. The patients received additional metformin (500 mg/day) or sitagliptin (50 mg/day) for 6 months, and changes in metabolic parameters including MDA-LDL were evaluated. Results: After 6 months of treatment, add-on sitagliptin (n=35) improved fasting blood glucose (FBG) and hemoglobin A1c (HbA1c) to significantly greater extent than increasing the dose of metformin (n=35). There were no differences in total cholesterol and low-density lipoprotein cholesterol levels between two groups. MDA-LDL levels (mean±S.E.) decreased significantly with increasing the dose of metformin (from 94.40±6.35 to 77.83±4.74 U/L, P < 0.005), but remained unchanged with add-on sitagliptin treatment (from 89.94±5.59 to 98.46±6.78 U/L, p > 0.05). Multiple linear regression analysis identified increasing the dose of metformin treatment as the only independent factor associated with decreased MDA-LDL (β coefficient 0.367, P < 0.0119), and no significant correlation between change in MDA-LDL and fasting blood glucose or HbA1c. Conclusion: These results suggest that increasing the dose of metformin improves serum MDA-LDL levels in type 2 diabetes mellitus.

scholarly journals Myrmecodia platytyrea Methanol Tuber Extract Ameliorates Hyperglycemia In STZ-Induced Diabetic Sprague-Dawley Male Rats

2021 ◽  
pp. 338-348
Author(s):  
Mizaton Hazizul Hasan ◽  
Hasbullani Zakaria ◽  
Ibtisam Abdul Wahab ◽  
Thellie Ponto ◽  
Aishah Adam
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Male Rats ◽  
Current Therapy ◽  
Diabetic Patients ◽  
Sprague Dawley ◽  
Tuber Extract

Type 2 diabetes mellitus (T2DM) is one of the main non-communicable chronic diseases that has many complications that compromise the quality of life. Hence, the need to find alternatives to replace the current therapy or as an adjuvant. Tubers of Myrmecodia platytytrea (Rubiaceae) has been used traditionally as an alternative therapy for the management of cancer and other inflammatory-related disorders. The aim of this study was to investigate the potency of M. platytytrea methanolic tuber extract (MPMTE) as an antihyperglycemic agent, in vivo. :The streptozotocin (STZ)-induced diabetic rats were treated orally with MPMTE (100, 200 and 400 mg/kg) and metformin (positive control, 100 mg/kg) daily for 14 days. Blood glucose level and other biochemistry analysis were conducted including histological examination on liver, kidney and pancreas.  The STZ-induced diabetic rats treated with MPMTE (200 and 400 mg/kg) had significant decreased (p<0.05) in fasting blood glucose, total cholesterol, triglycerides and low-density lipoprotein (LDL) with no significant changes in high-density lipoprotein (HDL) compared to STZ-induced untreated diabetic rats. Liver, kidney and pancreas were devoid of any damage caused by STZ.  MPMTE had strong antihyperglycaemic activity and was protective against any STZ-induced organ damage. Thus, MPMTE can be further developed into an adjuvant therapy for diabetic patients.

scholarly journals Assessment of the salivary glucose as a non-invasive test for diabetic patients

2019 ◽  
Vol 6 (6) ◽  
pp. 2289
Author(s):  
Kajol Thapa ◽  
Saroj Kunwar ◽  
Sonu Thapa ◽  
Asmita Phuyal ◽  
Sahil Rupakheti
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Diabetic Patients ◽  
Glucose Levels ◽  
Non Invasive ◽  
Invasive Test ◽  
Blood Glucose Levels ◽  
Institutional Review

Background: Over the recent year there has been a startling rise in the number of people suffering from diabetes mellitus. Estimation of blood glucose levels has been an essential laboratory investigation for screening and monitoring of diabetes. Saliva is one of the secretions in human body whose collection is easy and non-invasive. Salivary glucose hence could serve as an easy and non-invasive tool.Methods: Institutional review committee of NHRC gave us permission to carry out this study. 105 subjects with Type 2 diabetes mellitus who attended the OPD at Star hospital, Sanepa, Lalitpur, Nepal and 106 healthy individuals were consented to participate in this study. Glucose was measured by the GOD-POD (Glucose oxidase peroxidase) methods using the semi-autoanalyser and salivary glucose was compared with corresponding blood glucose levels.Results: A significant positive correlation of fasting salivary glucose level and fasting blood glucose level was observed in healthy (r=0.241, p=0.001) and in diabetic patients (r=0.202, p=0.001).Conclusions: The study implies a potential for saliva in monitoring and screening of diabetes mellitus.

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