Isolation and identification of probiotic bacteria from Iranian traditional cheese produced by lamb or kid abomasum microorganisms

Author(s):  
Iraj Hasani ◽  
Abbas Akhavan Sepahi ◽  
Shahram Shoeibi ◽  
Gholamhassan Asadi Aghdash

Different strains of lactic acid bacteria are the most common micro-organisms known as probiotics. Two major kinds of probiotic bacteria are members of the genera Lactobacillus and Bifidobacterium, that are generally associated with the genus Lactobacillus which play a vital role in the body health, as well as the ability to produce antimicrobials and vitamins in the gastrointestinal tract. The purpose of the study was the isolation of probiotics in traditional cheeses that are produced from the natural flora of the newborn mammalian digestive tract. Bacteria from lamb and kid abomasum are added to milk. The curd is slaughtered from the abomasum of a lamb or kid that is suckling and has now no longer began out to devour forage, after which its belly is dried, that is referred to as curd. In this study, sixteen samples of cheeses local to Markazi province of Iran have been prepared. The isolated bacteria have been examined for morphological, biochemical, probiotic properties, and molecular identity. Out of sixteen traditional cheese samples, five kinds of Lactobacillus have been diagnosed through PCR. L. casei strain J026 strain was identified in the traditional cheese samples as the most genera. Using probiotic starters from local sources is a beneficial strategy for producing traditional cheese, which the native strains are more compatible with the humans’ intestinal flora and therefore may also higher play their probiotic’s characteristic.

2018 ◽  
Vol 24 (27) ◽  
pp. 3223-3231 ◽  
Author(s):  
Luyao Li ◽  
Shiyao Xu ◽  
Tingting Guo ◽  
Shouliang Gong ◽  
Chuan Zhang

Objective: To investigate the effect of dapagliflozin on intestinal microflora in MafA-deficient mice using an animal model of diabetes. Methods: Male MafA-deficient mice were administered dapagliflozin (1.0 mg/kg/d) intragastrically for 6 weeks. Mouse body weights and fasting blood glucose levels were measured, and intestinal short-chain fatty acids were measured by gas chromatography. A series of methods was used to analyse the number of primary harmful bacteria in the faeces, and high-throughput sequencing was used to sequence the changes in intestinal flora. Results: The weight of the mice decreased after dapagliflozin gavage, and fasting blood glucose was significantly lower than that in the control group (P < 0.001). Acetic acid and butyric acid contents in the intestinal tracts of the mice increased, and the growth of harmful microorganisms, such as Clostridium perfringens, enterococci, Enterobacteriaceae, and intestinal enterococci, was inhibited. Blautia is a species found in the experimental group and was significantly different from the control and blank groups as determined by the LDA score from highthroughput sequencing. Conclusion: Dapagliflozin can reduce fasting blood glucose, decrease body weight, increase short-chain fatty acid content, regulate the intestinal microecological balance of the body and promote blood glucose and energy homeostasis.


2021 ◽  
Vol 22 (15) ◽  
pp. 7931
Author(s):  
Ning Liu ◽  
Shiqiang Sun ◽  
Pengjie Wang ◽  
Yanan Sun ◽  
Qingjuan Hu ◽  
...  

Serotonin, also known as 5-hydroxytryptamine (5-HT), is a metabolite of tryptophan and is reported to modulate the development and neurogenesis of the enteric nervous system, gut motility, secretion, inflammation, sensation, and epithelial development. Approximately 95% of 5-HT in the body is synthesized and secreted by enterochromaffin (EC) cells, the most common type of neuroendocrine cells in the gastrointestinal (GI) tract, through sensing signals from the intestinal lumen and the circulatory system. Gut microbiota, nutrients, and hormones are the main factors that play a vital role in regulating 5-HT secretion by EC cells. Apart from being an important neurotransmitter and a paracrine signaling molecule in the gut, gut-derived 5-HT was also shown to exert other biological functions (in autism and depression) far beyond the gut. Moreover, studies conducted on the regulation of 5-HT in the immune system demonstrated that 5-HT exerts anti-inflammatory and proinflammatory effects on the gut by binding to different receptors under intestinal inflammatory conditions. Understanding the regulatory mechanisms through which 5-HT participates in cell metabolism and physiology can provide potential therapeutic strategies for treating intestinal diseases. Herein, we review recent evidence to recapitulate the mechanisms of synthesis, secretion, regulation, and biofunction of 5-HT to improve the nutrition and health of humans.


2021 ◽  
Vol 22 (11) ◽  
pp. 5589
Author(s):  
Patrick C. Baer ◽  
Benjamin Koch ◽  
Helmut Geiger

The kidneys play a vital role in the basic physiological functions of the body [...]


1924 ◽  
Vol 23 (1) ◽  
pp. 106-116 ◽  
Author(s):  
Leonard P. Lockhart

1. The appearance and disappearance of typhoid bacilli in the faeces in these four cases bore no relation to changes in the diet nor to the physical state of the stools, but the later they appeared the shorter was the duration of their appearance.On the disappearance of typhoid bacilli from the stools the intestinal flora tended to become more simple.2. B. coli was the only organism invariably present at every examination.3. Streptococci were very much more abundant in the earlier stages of the disease, when milk formed the greater part of the diet, than in the later stages.4. In two cases where boils occurred on the body the causative organism had previously been isolated in large numbers from the faeces.5. With stools slightly alkaline to litmus the flora in these cases was relatively simple and fermentative in type. There is no apparent advantage, therefore, in giving a high carbohydrate diet except in cases of marked alkalinity and putrefaction.


2014 ◽  
Vol 49 (5) ◽  
pp. 647-653 ◽  
Author(s):  
Ann M. Cools ◽  
Tanneke Palmans ◽  
Fredrik R. Johansson

Context Tennis requires repetitive overhead movements that can lead to upper extremity injury. The scapula and the shoulder play a vital role in injury-free playing. Scapular dysfunction and glenohumeral changes in strength and range of motion (ROM) have been associated with shoulder injury in the overhead athlete. Objective To compare scapular position and strength and shoulder ROM and strength between Swedish elite tennis players of 3 age categories (&lt;14, 14–16, and &gt;16 years). Design Cross-sectional study. Setting Tennis training sports facilities. Patients or Other Participants Fifty-nine adolescent Swedish elite tennis players (ages 10–20 years) selected based on their national ranking. Main Outcome Measure(s) We used a clinical screening protocol with a digital inclinometer and a handheld dynamometer to measure scapular upward rotation at several angles of arm elevation, isometric scapular muscle strength, glenohumeral ROM, and isometric rotator cuff strength. Results Players older than 16 years showed less scapular upward rotation on the dominant side at 90° and 180° (P &lt; .05). Although all absolute scapular muscle strength values increased with age, there was no change in the body-weight–normalized strength of the middle (P = .9) and lower (P = .81) trapezius or serratus anterior (P = .17). Glenohumeral internal-rotation ROM and total ROM tended to decrease, but this finding was not statistically significant (P = .052 and P = .06, respectively). Whereas normalized internal-rotator strength increased from 14 to 16 years to older than 16 years (P = .009), normalized external-rotator and supraspinatus strength remained unchanged. Conclusions Age-related changes in shoulder and scapular strength and ROM were apparent in elite adolescent tennis players. Future authors should examine the association of these adaptations with performance data and injury incidence.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Tehmina Amjad ◽  
Mehwish Sabir ◽  
Azra Shamim ◽  
Masooma Amjad ◽  
Ali Daud

PurposeCitation is an important measure of quality, and it plays a vital role in evaluating scientific research. However, citation advantage varies from discipline to discipline, subject to subject and topic to topic. This study aims to compare the citation advantage of open access and toll access articles from four subfields of computer science.Design/methodology/approachThis research studies the articles published by two prestigious publishers: Springer and Elsevier in the author-pays charges model from 2011 to 2015. For experimentation, four sub-domains of computer science are selected including (a) artificial intelligence, (b) human–computer interaction, (c) computer vision and graphics, and (d) software engineering. The open-access and toll-based citation advantage is studied and analyzed at the micro level within the computer science domain by performing independent sample t-tests.FindingsThe results of the study highlight that open access articles have a higher citation advantage as compared to toll access articles across years and sub-domains. Further, an increase in open access articles has been observed from 2011 to 2015. The findings of the study show that the citation advantage of open access articles varies among different sub-domains of a subject. The study contributed to the body of knowledge by validating the positive movement toward open access articles in the field of computer science and its sub-domains. Further, this work added the success of the author-pays charges model in terms of citation advantage to the literature of open access.Originality/valueTo the best of the authors’ knowledge, this is the first study to examine the citation advantage of the author-pays charges model at a subject level (computer science) along with four sub-domains of computer science.


2020 ◽  
Vol 8 (9) ◽  
pp. 4426-4430
Author(s):  
Bhuvnesh Sharma ◽  
Gyanendra Datta Shukla ◽  
Parul Sharma

Kidneys plays a vital role in homeostasis by maintaining the body fluid and removing the harmful toxins out of the body through urine formation. Chronic kidney disease is a type of kidney disease which is pro-gressive in nature and there is gradual loss of kidney function over a period of months or years and have complex and different etio-pathologies. With the growing number of cases and keeping in mind the availa-ble conventional line of treatment and its financial burden on patient, Ayurveda through its holistic line of management, either in the form of Shamana & Shodhana or in the form of dietary advices stand distinct and it seems to be effective and safe. And by including proper daily dietary rules and regimens in patients of Chronic kidney disease (CKD) it can be very effectively managed. Here Trinpanchmool, Punarnava, Gokshur used in Basti. Basti plays a vital role here in CKD, it helps in expelling out the uremic toxins which originates in gut thereby improving the quality of life and reducing the sign and symptoms of dis-ease.


2021 ◽  
pp. 205-219
Author(s):  
Ahmad Roumiani ◽  
Taghi Ebrahimi Salari ◽  
Hamideh Mahmoodi ◽  
Mofid Shateri

This article evaluates nine rural districts in Kuhdasht county, Iran, with a population of 3535 between 2013–2016. We address the following two questions: First, what are the most important criteria and effective indicators in the rural population’s quality life enhancement? Second, is there any significant relationship between the public space indicators and quality life enhancement in the case study area? Six factors, including perceptual vision, buildings skeletons, culture and communities, activities, social interaction, and the environment from local peoples’ perspectives, explained 52.6 percent of the total variable variances. The Friedman test showed a significant difference among criteria of esthetics, semantic-perceptual, and activity-based functional at the alpha level of 0.01. The fitting growth regression model showed that the positive effect of the public space indicators on the rural population’s vitality and dynamism quality enhancement was about 0.723, indicating a significant relationship between them. It also stated a vital role of public space indicators in the rural population’s vitality and dynamism quality enhancement in the study area. The most important indicators were those of economic, social, and cultural dynamism and the body and space indicators.


2021 ◽  
Vol 3 (4) ◽  
pp. 197-205
Author(s):  
Dharmalingam T.S ◽  
Balachandran P

In the modern world, the yoga plays a vital role in human life. To realise the truth and meaning of life, one has to practice yogic discipline in everyday living. The human body is made up of five elements such as akash, air, fire, water and earth. An air plays a very important role to survive by providing life energy. Everyday practice of pranayama would help in regulating respiratory system (Breathing exercise) as well as circulatory system. The physical exercises regulate the circulation of blood, heat, air in the body. The practice of asana would help in bringing flexibility in our muscular system so as to enhance the meditation process. The practice of meditation gives peace of mind and improves integirity. The practice of yoga will improve self-confidence, self-control, speaking truth, tolerance and social virtues. Each and every individual should practice yoga, asanas, meditation and do self-analysis exercises such as analysis of thoughts, moralisation of desires, nutralisation of anger and eradication of worries. This would help to bring hormoney between body and mind, habit and wisdom, self and society. Purpose and method and will and nature.


2018 ◽  
Vol 26 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Barbara Kazuń ◽  
Krzysztof Kazuń ◽  
Joanna Żylińska ◽  
Andrzej K. Siwicki

Abstract The presence of lactic acid bacteria (LAB) favors the stabilization of intestinal flora, facilitates digestion, improves the assimilability of fodder, and has an immunomodulatory effect on the immune system. According to current research, the application of LAB following antibiotic treatment prevents the development of opportunistic bacteria inhabiting the digestive tract. In the study the potential probiotic properties of Lactobacillus plantarum strains, which can be administered as an alternative to antibiotic treatment in aquaculture, were investigated under in vitro conditions. The strains of L. plantarum were characterized for important properties such as the ability to grow in the presence of 10% fish bile, a tolerance of low pH, and antagonism to pathogens dangerous for fish such as Aeromonas salmonicida and Pseudomonas fluorescens; therefore, they meeting the criteria for strains with probiotic properties. In view of currently increasing resistance to antibiotics and a decrease of their efficiency, probiotic bacteria can serve to support immunity to infections in the future.


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