scholarly journals ANTIMALARIAL POTENTIAL STUDY OF CUCUMBER SKIN AND BASE POINT CHLOROFORM EXTRACT AS A SOLUTION TO ORGANIC WASTE OF SIDEWALK FOOD STALL TREATMENT IN YOGYAKARTA

2015 ◽  
Vol 2 (1) ◽  
pp. 168
Author(s):  
Imam Fathoni ◽  
Isnawan Ibnu Ikrandita ◽  
M. Dylan Lawrie ◽  
Eza Darisqi ◽  
Gede Wahyu Mega Udayana ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Drug ◽  
Base Point ◽  
Chloroform Extract ◽  
Negative Control ◽  
Control Treatment ◽  
Fruit Peel ◽  
Standard Drug ◽  
Cucumis Sativa ◽  
The City

<p>Malaria is a major infectious disease in the world. The disease is caused by blood protozoan from the genus Plasmodium. The main problem in controling this disease is resistance parasite cases to drugs that have been used. Cucumber (Cucumis sativa L.) contain bioactive compounds suspected of terpenoids and saponins are believed to reduce the level of parasitemia. Waste of skin and cucumber base is very abundant, especially from stalls in the city of Yogyakarta. Estimated at the base and cucumber skin there are bioactive content that can be used as an alternative antimalarial drug. This research aims to study the potential of chloroform extract and the base of the fruit peel waste to the level of parasitemia of Plasmodium berghei in mice. <br />First step is collecting the waste of skin and cucumber base in some stalls in the city of Yogyakarta. Extraction by maceration method using chloroform solvent, the method further phytochemical studies by Thin Layer Chromatography (TLC). Antiplasmodium test with negative control treatment (DMSO 0.3%), positive control (chloroquine 3 mg / kg), the dose C.sativa extract 100; 200; 300; 400 and 500 mg / kg in male mice given strain Switzerland 3 months of age infected with Plasmodium berghei orally. T he results showed there were terpenoids and saponins compounds in the chloroform extracts of C.sativa base and skin. The most effective dose of the extract inhibition of parasitemia level P. berghei in mice treated P5 is the highest (500 mg / kg BB), which is still higher than the standard drug Chloroquine so that waste of skin and the base C.sativa potential as an alternative antimalarial drug.</p><p><br /><strong>Keywords</strong>: Waste of skin and the base of cucumber (Cucumis sativa L.) Antimalarial.</p>

scholarly journals In-vivo Antiplasmodial Activity of Sulfadoxine/Pyrimethamine/Doxycycline on Plasmodium berghei Infected Mice

2021 ◽  
pp. 1-8
Author(s):  
Udeme Owunari Georgewill ◽  
Elias Adikwu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Antimalarial Drug ◽  
Low Density Lipoprotein ◽  
White Blood Cells ◽  
Density Lipoprotein ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Lipoprotein Cholesterol

The search for newer antimalarial drug combinations is on the front burner due to rising Plasmodium resistance to some currently used antimalarial drugs. This study examined the antiplasmodial activity of sulfadoxine/pyrimethamine/doxycycline (S/P/D) on mice infected with Plasmodium berghei (P. berghei). Swiss albino mice (25-30 g) inoculated with P. bergei (1x107) were treated with D (2.2 mg/kg), S/P (21.4/10.7 mg/kg), and S/P/D for 4 days. The positive and negative controls were treated with normal saline (0.2 ml) and chloroquine (CQ) (10 mg/kg) for 4 days, respectively. After treatment, blood samples were collected and assessed for parasitemia levels and biochemical parameters. The mice were observed for mean survival time (MST). D, S/P, S/P/D and CQ significantly decreased parasitemia in the curative, prophylactic and suppressive tests at p<0.05; p<0.01, p<0.001 and p<0.001, respectively when compared to negative control. In the curative study, 55.9%, 65.1%, and 81.7% parasitemia inhibitions were produced by D, S/P and S/P/D, respectively whereas CQ produced 75.6 % parasitemia inhibition. D, S/P and S/P/D significantly prolonged MST at p<0.05, p<0.01 and p<0.001 respectively when compared to negative control. Altered serum biochemical markers in  P. berghei infected mice were marked by  significantly (p<0.001) decreased  packed cell volume, red blood cells, hemoglobin, high density lipoprotein cholesterol levels with  significantly (p<0.001) increased cholesterol, white blood cells, total cholesterol, low-density lipoprotein cholesterol and triglyceride levels when compared to control. However, D, S/P and S/P/D significantly restored the aforementioned markers at p<0.05, p<0.01 and p<0.001, respectively when compared to negative control. S/P/D may be used as an antimalarial drug.

scholarly journals Ethanol Root-extract of Sphenocentrum jollyanum Restored Altered Haematological Markers in Plasmodium berghei-infected Mice

2019 ◽  
pp. 189-203
Author(s):  
E. U. Ekpono ◽  
P. M. Aja ◽  
U. A. Ibiam ◽  
E. U. Alum ◽  
U. E. Ekpono
Keyword(s):  
Body Weight ◽  
Blood Cell ◽  
Normal Control ◽  
Plasmodium Berghei ◽  
Negative Control ◽  
Haematological Parameters ◽  
Pack Cell Volume ◽  
Control Group ◽  
Root Extract ◽  
Standard Drug

Effect of ethanol root-extract of Sphenocentrum jollyanum on some haematological parameters in Plasmodium berghei-infected mice was carried out using a total of thirty-six albino mice. The mice were randomly assigned into six experimental groups of A-F with six mice in each group. Mice in group A (Normal control) were administered with normal saline. Mice in groups B-F were infected with Plasmodium berghei intraperitoneally. Mice in group B (Positive control) were treated with 5 mg/Kg body weight of standard drug; mice in group C (Negative control) were left without treatment while mice in groups D, E and F were treated with graded doses of 200, 400 and 800 mg/Kg body weight of the ethanol root extract of Sphenocentrum jollyanum, respectively. All the mice were allowed access to water and feed without restriction. Oral route was used for the administration of the standard drug and plant extract. The treatment lasted for ten days. Percentage parasitaemia was determined on the 3rd day and last day after infection. Evaluations of haematological parameters were done using spectrophotometric method while percentage parasitaemia count was done using microscopic method. Treatment of the infected mice with the ethanol root extract of Sphenocentrum jollyanum reduced parasite count significantly (P < 0.05). Infection of mice with Plasmodium berghei caused a significant (P < 0.05) decrease in the levels of pack cell volume (PCV), haemoglobin (HB), red blood cell (RBC) and white blood cell (WBC) count relative to the normal control. However, treatment of P.berghei-infected mice with the ethanol root extract of Sphenocentrum jollyanum at the doses of 200, 400 and 800 mg/Kg body weight of the mice showed a dose-dependent significant (P < 0.05) reversal in the trend of these parameters to a level comparable to that observed among the standard control group, especially, the highest dose of 800 mg/Kg body weight. This study indicates that ethanol root extract of Sphenocentrum jollyanum could be used in the management of anaemic diseases associated with Plasmodium berghei infection.

scholarly journals Antiplasmodial, Antipyretic, Haematological and Histological Effects of the Leaf Extracts of Moringa oleifera in Plasmodium berghei berghei Infected Mice

2019 ◽  
pp. 1-13
Author(s):  
O. Olaniran ◽  
F. C. Adetuyi ◽  
F. O. Omoya ◽  
S. A. Odediran ◽  
R. E. Hassan-olajokun ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Plasmodium Berghei ◽  
Moringa Oleifera ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Haematological Parameters ◽  
Standard Drug ◽  
Antipyretic Activity ◽  
Ethanolic Extracts

The aqueous and ethanolic extracts of Moringa oleifera leaf were evaluated for safety, antimalarial and antipyretic activities because it is commonly used for various types of ailments especially malaria and fever. The various concentration of these extracts (0-800mg/kg) were tested against Plasmodium berghei berghei infected albino mice of either sex, in a Peters’ four day antiplasmodial test while antipyretic activities were evaluated in malaria- induced mice. Their possible effects on haematological parameters of PCV, WBC, neutrophil, lymphocytes count and liver enzymes were also determined as well as on the liver and kidney architecture. All the tested doses of the aqueous extract were comparable in antiplasmodial activity (p>0.05) to each of the tested doses of the ethanolic extract which were significantly different (p<0.05) from the negative control.  Only the highest doses of both extracts gave significantly higher (p<0.05) antiplasmodial activity than the standard drug. For the ethanolic extract, a significant antipyretic activity was not observed until at 800mg/kg in time T3 while the aqueous extract exerted no significant antipyretic activity at all doses and at all times. The ethanolic extract gave significantly higher PCV values than that of the aqueous. The administration of the aqueous extract gave significantly lower WBC than the ethanolic extract while comparable increase in lymphocyte count was noticeable at each of the doses of both extracts when compared to the negative control. Only 100mg/kg and 800mg/kg of the aqueous extract maintained a relatively high neutrophils count in this study. Also, the aqueous extract elicited higher concentration of ALT (greater than fourfold of normal) but the ethanolic extract produced the highest level of AST at the highest dose of 800mg/kg when compared to the ethanolic extract. Histological examination of the kidney showed progressive degeneration while that of the liver showed recovery when compared to negative control as a result of progressive increase in dosage of the extracts in malarial treatment. In conclusion, though both aqueous and ethanolic extracts of M. oleifera leaves exert chemosuppressive antiplasmodial activities in Plasmodium berghei berghei infected mice and exhibited antipyretic activity with some improved haematological parameters, caution should be taken in its indiscriminate use because of probable toxic effects on the kidney and the liver.

Structure-switching aptamer sensors for the specific detection of piperaquine and mefloquine

2021 ◽  
Vol 13 (585) ◽  
pp. eabe1535
Author(s):  
Erin S. Coonahan ◽  
Kyung-Ae Yang ◽  
Stevan Pecic ◽  
Maarten De Vos ◽  
Thomas E. Wellems ◽  
...  
Keyword(s):  
Drug Use ◽  
Antimalarial Drug ◽  
Dissociation Constants ◽  
Fluorescent Sensors ◽  
Specific Detection ◽  
Standard Drug ◽  
Sensing Platforms ◽  
Current Spread ◽  
Structure Switching ◽  
Exponential Enrichment

Tracking antimalarial drug use and efficacy is essential for monitoring the current spread of antimalarial drug resistance. However, available methods for determining tablet quality and patient drug use are often inaccessible, requiring well-equipped laboratories capable of performing liquid chromatography–mass spectrometry (LC-MS). Here, we report the development of aptamer-based fluorescent sensors for the rapid, specific detection of the antimalarial compounds piperaquine and mefloquine—two slow-clearing partner drugs in current first-line artemisinin-based combination therapies (ACTs). Highly selective DNA aptamers were identified that bind piperaquine and mefloquine with dissociation constants (Kd’s) measured in the low nanomolar range via two independent methods. The aptamers were isolated from a library of single-stranded DNA molecules using a capture–systematic evolution of ligands by exponential enrichment (SELEX) technique and then adapted into structure-switching aptamer fluorescent sensors. Sensor performance was optimized for the detection of drug from human serum and crushed tablets, resulting in two sensing platforms. The patient sample platform was validated against an LC-MS standard drug detection method in samples from healthy volunteers and patients with malaria. This assay provides a rapid and inexpensive method for tracking antimalarial drug use and quality for the containment and study of parasite resistance, a major priority for malaria elimination campaigns. This sensor platform allows for flexibility of sample matrix and can be easily adapted to detect other small-molecule drugs.

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

2017 ◽  
Vol 2 (2) ◽  
pp. 147
Author(s):  
Triswanto Sentat ◽  
Susiyanto Pangestu
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Treatment Groups ◽  
Analgesic Effects ◽  
Significant Difference ◽  
Analgesic Dose ◽  
Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

scholarly journals The efficacy of plant extracts on cecal amebiasis in rats

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Enerst C. Ohanu ◽  
Paul C. Inyang-Etoh
Keyword(s):  
Body Weight ◽  
Plant Extracts ◽  
Public Health Problem ◽  
Parasite Load ◽  
Negative Control ◽  
Major Public Health Problem ◽  
Cure Rate ◽  
Standard Drug ◽  
The Mean ◽  
The Difference

Amebiasis caused by <em>Entamoeba histolytica</em> is a major public health problem in tropical and subtropical countries. Treatment failure with specific chemotherapy has been reported suggesting the possibility of drug resistance. This study investigated the anti-amoebic effects of four plant extracts on cecal amebiasis in rats. The cecal amebiasis was induced by the injection of 3.0×105 troph/mL of <em>E. histolytica</em> parasite directly into the rat’s caecum. A total of 137 rats were used for these studies; five rats in each group for both positive and negative control, 15 rats in each group to test the four plant extracts and metronidazole. The infected rats were treated for cecal amebiasis using each of the four plant extracts at graded doses of 100 mg/kg, 200 mg/kg and 400 mg/kg and with metronidzole at a dose of 62.5 mg/kg,100 mg/kg and 125 mg/kg for five consecutive days. The efficacy of the four plant extracts were evaluated based on Neal’s, 1951 method. The plant extracts of Garlic, <em>Guava</em>, Pawpaw and Pumpkin at 400 mg/kg and 200 mg/kg body-weight gave a cure rate of 80%, 100%, 60%, 40% and 40%, 80%, 40%, 0%, respectively. The mean parasite count in the cecal contents of the treated rats at a dose 400 mg/kg were 18.5±1.6, 0.0±0.0, 33.3±1.8 and 49.5±4.0, respectively. The difference was statistically significant (P&lt;0.05). This study has revealed that Guava at a high dosing level (400 mg/kg body weight) is as good as the standard drug in reducing the both parasite load (probably with limited side effect).

scholarly journals Sequestration of erythrocytes infected with Plasmodium berghei ANKA in BALB/c mice treated with goat bile

2020 ◽  
Vol 4 (2) ◽  
pp. 179
Author(s):  
Kartika Arum Wardani ◽  
Kholida Nur Aini ◽  
Heny Arwati ◽  
Willy Sandhika
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Control Group ◽  
Dependent Manner ◽  
Plasmodium Berghei Anka ◽  
Concentration Dependent Manner ◽  
Control Group Design ◽  
Bile Concentration

Abstract Sequestration of Plasmodium berghei ANKA-infected erythrocytes occurs in BALB/c mice as characteristic of  Plasmodium falciparum infection in humans. Animals’ bile has been widely used for centuries in Traditional Chinese Medicine. Goat bile has been used in healing infectious and non-infectious diseases; however, no report on the use of goat bile against malaria infection and sequestration. The purpose of this study was to analyze the correlation between parasitemia and sequestration in the liver of P.berghei ANKA-infected BALB/c mice treated with goat bile. This research was an in vivo experimental study using the post-test control group design. The male BALB/c mice aged ± 6 weeks, body weight 20-25 g were used. The mice were divided into five groups where Group 1-3 were mice treated with goat bile 25%, 50%, and 100%, respectively. Group 4-5 were negative (sterile water) and positive controls (DHP). Parasitemia was observed daily from each mouse and the number of sequestered infected erythrocytes on the endothelium of sinusoids. The data were analyzed using t independent test. Antimalarial activity of goat bile was shown by the lower parasitemia in goat bile-treated mice compared with the negative control. The average number of sequestration was goat bile concentration-dependent manner. The higher the concentration, the lower the number of sequestration. Sequestration was correlated with parasitemia (p=0,0001). Sequestration of P.berghei ANKA-infected erythrocytes correlated with parasitemia, and was goat bile concentration-dependent manner. Keywords: Malaria, parasitemia, sequestration, goat bileCorrespondence: [email protected]

scholarly journals GAMBARAN HISTOPATOLOGI PARU TIKUS WISTAR SETELAH DIBERI PAPARAN ASAP ROKOK

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Novera Herdiani ◽  
Endah Budi Permana Putri
Keyword(s):  
Oxidative Stress ◽  
Alveolar Macrophage ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Rat Lung ◽  
Dragon Fruit ◽  
Lung Histopathology

Abstract: Cigarette smoke is the main cause of lung obstruction. One of the obstructions in real that iscaused by cigarette smoke is oxidative stress. Oxidative stress trigger inflammation response occur andlung obstruction. Cigarette smoke able to cause lung histopathology changes like lungs obstruction onalveolus wall. Red dragon fruit extract tackle free radical then able to against the oxidative stress. Theobjective of this study to examines the image of rat lung histopathology under exposed cigarette smoke.Twenty four Wistar rats divided four groups: negative control, positive control, red dragon fruit extracttreatment of 7,2 g/200 g WB, and red dragon fruit extract10,8 g/200 g WB. Negative control only givenstandard feed. Positive control given standar feed and exposed 21 cigarette per day. Treatment groupgiven feed during 21 days. In the 22nd day rats be sacrificed, the lung taken out for observation andimage of rat lung histopathology changes by making lung organ histopathology preparationhematoxicillin Eosion (HE) staining and observed under magnification light microscope 400x. The endresult of the study indicate the finding of changes in lung histopathology such as obstruction level overthe lung tissue higher, alveolar macrophage covered alveoli after being exposed cigarette smoke. Groupwhich does not exposed cigarette smoke, lung alveolus macrophage in normal condition, there are noobstruction or alveolus macrophage occurred, its alveolar macrophage does no covering alveoli.Treatment group administered fruit dragon extract dose 10,8 g/200 g WB and dose 7,2 g/200 g WB seemalmost the same with negative control treatment. Conclusion of the research is cigarette smoke exposecan influence the number of alveolar macrophage on wistar rats. Suggestion very required furtherresearch on oxdative stress parameter.

scholarly journals First Report of Xanthomonas arboricola pv. pruni in Ornamental Prunus laurocerasus in the Netherlands

Plant Disease ◽  
2012 ◽  
Vol 96 (5) ◽  
pp. 759-759 ◽  
Author(s):  
N. N. A. Tjou-Tam-Sin ◽  
J. L. J. van de Bilt ◽  
M. Bergsma-Vlami ◽  
H. Koenraadt ◽  
J. Westerhof Naktuinbouw ◽  
...  
Keyword(s):  
The Netherlands ◽  
Genotypic Variation ◽  
Negative Control ◽  
Control Treatment ◽  
Tissue Paper ◽  
Field Isolates ◽  
Leaf Spots ◽  
Xanthomonas Arboricola ◽  
Reference Strains ◽  
Prunus Laurocerasus

In 2008, Dutch ornamental plant growers observed a leaf spot of cherry laurel (Prunus laurocerasus) at a greater incidence (5 to 50%) than the usual sporadic level (<1%). For advice on disease control, ~5 to 10% of these growers contacted Dutch regulatory officials. In November and December 2008, six symptomatic samples from northern and southern parts of the Netherlands were submitted for diagnosis. Leaf spots were chlorotic, most had a necrotic brown center with a distinct margin, and the spots readily abscised, resulting in a “shot-hole” appearance. Leaf spots from the samples were surface sterilized (2 s in 70% vol/vol alcohol), blotted dry on tissue paper, chopped into pieces (1 to 2 mm in diameter), and incubated for 30 min in 10 mM phosphate-buffered saline (PBS) (1). A 20-μl aliquot of extract per sample was streaked by dilution plating on four plates of yeast peptone glucose agar medium (1), and the plates were incubated for 2 to 3 days at 28°C. Isolations from all six samples yielded Xanthomonas-like colonies. After purification, characterization of all six isolates revealed oxidative, nonfermentative metabolism of glucose by rod-shaped, gram-negative bacterial cells. All six isolates were identified as Xanthomonas arboricola pv. pruni based on biochemical tests (1), fatty acid analysis (4), and immunofluorescence (IF) using polyclonal antibodies (Plant Research International, the Netherlands). Pathogenicity was tested on potted peach plants (cvs. Peregrine and Vaes Oogst) and on detached leaves of P. laurocerasus (cv. Novita) (1). The six field isolates from 2008 were each inoculated (108 CFU/ml) onto four leaves per plant of each of two peach plants (replicates). As positive control treatments, two reference strains (ATCC 19312 and PD740) were each inoculated onto the same number of leaves and plants, and as a noninoculated negative control treatment, leaves of two peach plants were treated with sterile 10 mM PBS buffer (1). All leaves inoculated with the six field isolates and the two reference strains developed typical bacterial spot symptoms in 3 to 4 weeks. Negative control plants showed no symptoms. The detached leaf assay performed with the same treatments on each of two leaves (replicates) showed identical results. The bacterium was reisolated from leaf spots associated with each of the eight symptomatic treatments and identity of the reisolates was confirmed by IF. Additionally, genotypic variation of 35 Dutch isolates of X. arboricola pv. pruni was assessed by BOX-PCR assay with the BOX A1R primer set (3), and Gyrase B gene sequencing (2). Both methods revealed 100% homology among the 35 isolates, suggesting a single, recent introduction of X. arboricola pv. pruni into the Netherlands. In a 2009 survey to assess distribution of the disease in the Netherlands, X. arboricola pv. pruni was found in 41 fields. Infected hosts included P. laurocerasus cvs. Otto Luyken, Rotundifolia, Novita, Etna, Anbri, Herbergii, Mischeana, and Caucasia. X. arboricola pv. pruni is a quarantine organism in countries affiliated under the EPPO (European and Mediterranean Plant Protection Organization). Phytosanitary measures were taken to prevent movement of infested plants from nurseries where X. arboricola pv. pruni was detected. References: (1) Anonymous. EPPO Bull. 36:129, 2006. (2) N. Parkinson et al. Int. J. Syst. Evol. Microbiol. 59:264, 2009. (3) J. Versalovic et al. Methods Mol. Cell. Biol. 5:25, 1994. (4) S. A. Weller et al. EPPO Bull. 30:375, 2000.

scholarly journals In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

2021 ◽  
pp. 12-20
Author(s):  
Udeme O. Georgewill ◽  
Festus Azibanigha Joseph ◽  
Elias Adikwu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Positive Control ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Significant Difference ◽  
Tract Infections

Nitrofurantoin (NT) used for the treatment of urinary tract infections may have antiplasmodial activity. Dihydroartemisinin-piperaquine (DP) is an artemisinin based combination therapy used for the treatment of malaria. This study evaluated the antiplasmodial effect of dihydroartemisinin-piperaquine-nitrofurantoin (DP-NT) on mice infected with Plasmodium berghei. Adult Swiss albino mice (30-35 g) of both sexes were used. The mice were randomly grouped, inoculated with Plasmodium berghei, and treated orally with DP (1.7/13.7 mg/kg), NT (57.1 mg/kg) and DP-NT (1.71/13.7/ 57.1 mg/kg), respectively using curative, prophylactic and suppressive tests. The negative control was orally treated with normal saline (0.3 mL), while the positive control was orally treated with chloroquine CQ (10mg/kg). After treatment, blood samples were collected and evaluated for percentage parasitemia, inhibitions and hematological parameters. Liver samples were evaluated for histological changes. The mice were observed for mean survival time (MST). Treatment with DP-NT decreased parasitemia levels when compared to individual doses of DP and NT with significant difference observed at p<0.05. DP-NT prolonged MST when compared to individual doses of DP and NT with significant difference observed at p<0.05. The decrease in packed cell volume, red blood cells, hemoglobin and increase in white blood cells in parasitized mice were significantly restored by DP-NT  when compared to individual doses of DP and NT with difference observed at p<0.05. DP-NT eradicated liver Plasmodium parasite.  NT remarkably increased the antiplasmodial activity of DP. DP-NT may be used for the treatment of malaria.

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