scholarly journals Comparison of two doses of eCG for obtaining embryos in llama

SPERMOVA ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 173-178
Author(s):  
Cesar A Olaguivel ◽  
◽  
Jaime Ruiz ◽  
Pedro Coila
Keyword(s):  
Statistical Difference ◽  
Poor Quality ◽  
Dominant Follicle ◽  
Linear Transducer ◽  
Significant Difference ◽  
National University ◽  
Applied Dose ◽  
T1 And T2 ◽  
Ovulatory Follicles

The study was developed at the Pampa del Arco Experimental Center of the National University of San Cristóbal de Huamanga, the objective was to evaluate doses of 800 IU (T1) and doses of 1000 IU (T2) of eCG to obtain embryos. Eight adult female llamas were used, distributed four in each treatment (T1 and T2), with a body condition of 2.5, these were evaluated by ultrasound with a 7.5 MHz rectal linear transducer. Hormonal treatments began when the animals had a follicle> 7 mm. The application of eCG was 72 hours after the induction of ovulation of the dominant follicle with GnRH, on day 7 PGF2α was applied, natural mounting was performed plus GnRH (1ml), on day 15 the collection and evaluation of embryos was performed. The results obtained were the pre-ovarian stimulation follicular diameter of 8.3 mm and 9.1 mm for T1 and T2 respectively, the number of pre-ovulatory follicles was 6.33 and 5.50 for T1 and T2 respectively, the diameter of follicles pre-ovulatory was 11.2mm and 10.6 mm for T1 and T2 respectively, average of embryos recovered per donor was 4.66 ± 0.81 and 3.50 ± 0.54 embryos for T1 and T2 respectively, with a significant difference (p <0.05) and the quality of embryos had 35.7% and 38.1% of embryos of excellent quality, 28.6% and 33.3% of good quality, 10.7% and 9 , 5% of regular quality, 10.7% and 0% of poor quality, for T1 and T2 respectively and 14.3% and 19.0% of non-transferable embryos for T1 and T2 respectively, with no statistical difference (p≥0, 05). It is concluded that the number of embryos recovered with a dose of 800 IU of eCG is higher compared to the dose of 1000 IU of eCG (p <0.05) and that there is no association between the applied dose of eCG and the quality of the llama embryos (p≥0.05)

Do-It-Yourself Microsuture from Human Hair for Basic Microsurgical Training

2018 ◽  
Vol 35 (05) ◽  
pp. 315-321
Author(s):  
Pobe Luangjarmekorn ◽  
Pravit Kitidumrongsook ◽  
Sittisak Honsawek
Keyword(s):  
Human Hair ◽  
Statistical Difference ◽  
Smooth Curve ◽  
P Value ◽  
Excellent Quality ◽  
Likert Score ◽  
Significant Difference ◽  
Do It Yourself ◽  
Knot Security

Background Microsuture is an essential material for basic microsurgical training. However, it is consumable, expensive, and sometimes unavailable in the microsurgical laboratory. To solve this problem, we developed a microsuture made from human hair and needle gauge. Methods Do-It-Yourself (DIY) microsuture is made from human hair and needle gauge 32G (BD Ultra-Fine Pen Needles 4 mm × 32G). Methods are explained step by step. This DIY microsuture (labeled as “test microsuture”) and nylon 8–0 (Ethilon suture 8–0, labeled as “standard microsuture”) were used for teaching orthopaedic residents to perform arterial anastomosis in chicken thighs. All residents practiced without knowing that “test microsuture” was made from the DIY method. After completing the training, quality of both microsutures was evaluated by questionnaire in topics of (1) thread quality (size, strength, elasticity, handing, knot perform, and knot security), (2) needle quality (size, curve, shape, sharpness, handling, and strength), (3) needle–thread interface (size, strength, and smoothness), and (4) overall quality of microsuture. Each category was evaluated by Likert score (5 = excellent, 4 = good, 3 = fair, 2 = poor, and 1 = very poor). Results The DIY microsuture was performed in three steps: (1) insert human hair into needle gauge by microforceps, (2) bend needle into smooth curve, and (3) disconnect needle and create needle–hair interface. The questionnaire was completed by 30 orthopaedic residents and showed that thread quality of DIY and standard microsuture had “good” and “good-to-excellent” quality (mean Likert score: 3.77–4.23 and 3.80–4.27, respectively, with no statistical difference). Thread–needle interface quality of DIY and standard microsuture also had “good” and “good-to-excellent” quality (Likert score: 3.73–4.20 and 4.07–4.33, respectively, with no statistical difference). Needle part of DIY microsuture had lower quality than standard suture (fair-to-good compared with good-to-excellence quality, score 3.30–3.67 vs. 4.20–4.27, respectively, with a statistically significant difference, p-value < 0.05). However, overall quality of DIY suture and standard microsuture had “good” and “good-to-excellent” (mean Likert score: 3.73 and 4.00, respectively, with no statistical difference). Conclusion The DIY microsuture from human hair and needle gauge could be an alternative for basic microsurgical training with lower cost, easy production, and more availability for use in practice with acceptable quality compared with that of standard microsuture.

scholarly journals Slow Release Metformin Effect in Weight Loss and Total Calorie Intake in Obese Volunteers

2017 ◽  
Vol 3 (1) ◽  
pp. 50
Author(s):  
Cholis Abrori ◽  
Lucky Tiya ◽  
Deti Rosalina
Keyword(s):  
Weight Loss ◽  
Slow Release ◽  
Poor Quality ◽  
The Body ◽  
Calorie Intake ◽  
Measured Weight ◽  
Significant Difference ◽  
Total Calorie ◽  
Alternative Drug

Obesity can cause serious problems that will lead to poor quality of life, increased morbidity and mortality rate. Slow-release metformin will work longer in the body with a single dose everyday be an alternative drug for weight loss. The purpose of this study was to compare the effectiveness of slow-release metformin compared to metformin regular to body weight and calorie intake in volunteers with obesity. The study was conducted on 16 volunteers who were divided into two groups in pairs. Volunteers will be measured weight and calorie intake with a 24-hour food recall method, data was taken at the time before the intervention, the end of week 1st, 2nd, 3rd, and 4th. The result obtained weight loss better in the group receiving slow-release metformin by 5.08% compared to the group receiving regular metformin by 2,60%, but there was no significant difference between the groups in terms of calorie intake during the study. The conclusion of this study is a slow-release metformin is greater in weight loss than regular metformin, but does not affect the amount of calorie intake.Keywords: Metformin, slow-release, weight loss, calorie intake, obesity

scholarly journals P645 Mental health, work presenteeism and exercise in IBD patients

2021 ◽  
Vol 15 (Supplement_1) ◽  
pp. S577-S577
Author(s):  
M Sciberras ◽  
C Nascimento ◽  
T Tabone ◽  
K Karmiris ◽  
P Nikolaou ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Demographic Data ◽  
International Study ◽  
Poor Quality ◽  
Psychiatric Medication ◽  
Active Involvement ◽  
Significant Difference ◽  
Previous Medication ◽  
History Of

Abstract Background IBD has been shown to increase the rates of anxiety and depression amongst diagnosed individuals, with a prevalence rate of approximately 15- 20%. Chronic diseases such as IBD can have a significant impact on productivity at work (presenteeism). This can lead to emotional distress, poor quality of life and cost effects on employers. The primary aim of the study was to assess the prevalence of psychological problems, exercise levels and presenteeism at work among IBD patients. Methods This was a multicentre international study whereby IBD patients (&gt;18 years) in clinical remission over the last year, were asked to answer an anonymous questionnaire. Demographic data, type of IBD, current and previous medication, admissions to hospital, history of psychiatric referrals, Stanford Presenteeism Scale (SPS-6), Godin Score (exercise related score) were collected. Exclusion criteria included patients with IBD flares requiring corticosteroids in the previous 12 months. Results 573 patients (CD: n=318) from 8 European Centres and Israel participated in the study. The mean patient age was 39.9 years (SD+/- 13.0). 21.6% were smokers and 48.5% were non-smokers. The rest were ex-smokers. 10.9% of patients had a diagnosis of depression/anxiety prior to the diagnosis of IBD, whereas this increased to 20.6% post-IBD diagnosis, this being significantly commoner in the CD cohort (23.0%, p&lt;0.05) and in females (55.8%, p&lt;0.05). 37.7% of patients had been to a psychiatrist or a psychologist (41% of CD, p&lt;0.05) with 11.7% of patients being on psychiatric medication (14.5% of CD, p&lt;0.05). Low presenteeism at work was evident in 34.7%, with no statistically significant difference between UC and CD patients (p=0.5). 39.9% had a Godin Exercise Score being in the active range, 38.8% had a sedentary/insufficient exercise score. The rest were moderately active. Patients diagnosed with depression/anxiety had a more sedentary lifestyle. Conclusion In our study 37.7% of patients were referred for psychological help. This can have several effects including poor presenteeism at work (34.7%) and reduced efficiency. These issues are commoner in patients with CD than in UC. Active involvement of a psychologist/ psychiatrist as part of the IBD team should be routine as to improve the patient’s quality of life.

scholarly journals Psychiatric morbidity, quality of life and marital satisfaction among spouse of men with opioid dependence syndrome: a study from North India

2017 ◽  
Vol 4 (2) ◽  
pp. 556
Author(s):  
Ruchi Soni ◽  
Ritesh Upadhyay ◽  
Parth Singh Meena ◽  
Mahendra Jain
Keyword(s):  
Quality Of Life ◽  
Marital Satisfaction ◽  
Psychiatric Disorder ◽  
Opioid Dependence ◽  
Short Form ◽  
Psychiatric Morbidity ◽  
Poor Quality ◽  
North India ◽  
Significant Difference

Background: Opioid dependence syndrome has deleterious consequences not only on addict but also on the members of family especially his spouse who is most vulnerable to develop significant psychiatric disorder given the intimate nature of their relationship. Addressing these issues will be beneficial as spouses are important source of moral support and assistance to the substance user’s quest toward abstinence.Methods: For psychiatric morbidity, 100 spouses of men with opioid dependence syndrome were evaluated. Severity of opioid dependence in the husbands was assessed using severity of opioid dependence questioner (SODQ). Quality of life and marital satisfaction was assessed using short form health survey 36 (SF 36) and marital satisfaction scale (MSS) respectively.Results: Data analysis reveals that 33% of spouses had a psychiatric disorder. Primarily mood and anxiety disorder was present in 22% and 9% of subjects respectively. Highly significant difference existed between cases and controls in terms of marital satisfaction (p = 0.0001) and quality of life (p≤0.05) indicating low marital satisfaction and poor quality of life in spouses of opioid dependent individuals.Conclusions: Psychological distress and psychiatric morbidity in spouses of opioid dependent men is high, with poor quality of marital life and marital satisfaction being low. Hence, interventions that aim at allaying their distress and improving their mental health can improve the condition of the substance user and contribute to a better outcome of substance abuse treatment.

scholarly journals Nuss procedure for Pectus excavatum repair: critical appraisal of the evidence

2014 ◽  
Vol 41 (6) ◽  
pp. 400-405 ◽  
Author(s):  
Maria Elizete de Almeida Araújo ◽  
Anderson da Paz Penha ◽  
Fernando Luiz Westphal ◽  
Marcus Tolentino Silva ◽  
Taís Freire Galvão
Keyword(s):  
Observational Studies ◽  
Pectus Excavatum ◽  
Statistical Difference ◽  
Critical Appraisal ◽  
Evidence Synthesis ◽  
Poor Quality ◽  
Nuss Procedure ◽  
Assessment Development ◽  
Selection Of

Objective: To evaluate the effectiveness and safety of correction of pectus excavatum by the Nuss technique based on the available scientific evidence.Methods: We conducted an evidence synthesis following systematic processes of search, selection, extraction and critical appraisal. Outcomes were classified by importance and had their quality assessed by the Grading of Recommendations Assessment, Development and Evaluation (GRADE).Results: The process of selection of items led to the inclusion of only one systematic review, which synthesized the results of nine observational studies comparing the Nuss and Ravitch procedures. The evidence found was rated as poor and very poor quality. The Nuss procedure has increased the incidence of hemothorax (RR = 5.15; 95% CI: 1.07; 24.89), pneumothorax (RR = 5.26; 95% CI: 1.55; 17.92) and the need for reintervention (RR = 4.88; 95% CI: 2.41; 9.88) when compared to the Ravitch. There was no statistical difference between the two procedures in outcomes: general complications, blood transfusion, hospital stay and time to ambulation. The Nuss operation was faster than the Ravitch (mean difference [MD] = -69.94 minutes, 95% CI: -139.04, -0.83).Conclusion: In the absence of well-designed prospective studies to clarify the evidence, especially in terms of aesthetics and quality of life, surgical indication should be individualized and the choice of the technique based on patient preference and experience of the team.

scholarly journals The efficacy of Phaseolus vulgaris as a weight-loss supplement: a systematic review and meta-analysis of randomised clinical trials

2011 ◽  
Vol 106 (2) ◽  
pp. 196-202 ◽  
Author(s):  
Igho Onakpoya ◽  
Salsabil Aldaas ◽  
Rohini Terry ◽  
Edzard Ernst
Keyword(s):  
Systematic Review ◽  
Weight Loss ◽  
Clinical Trials ◽  
Meta Analysis ◽  
Poor Quality ◽  
Randomised Clinical Trials ◽  
Herbal Supplement ◽  
Significant Difference ◽  
Present Systematic Review

A variety of dietary supplements are presently available as slimming aids, but their efficacy has not been proven. One such slimming aid is the bean extract, Phaseolusvulgaris. The aim of the present systematic review is to evaluate the evidence for or against the efficacy of P. vulgaris. Electronic and non-electronic searches were conducted to identify relevant human randomised clinical trials (RCT). Hand searches of bibliographies were also conducted. No age, time or language restrictions were imposed. The eligibility of studies was determined by two reviewers independently, and the methodological quality of the included studies was assessed. We identified eleven eligible trials, and six were included. All the included RCT had serious methodological flaws. A meta-analysis revealed a statistically non-significant difference in weight loss between P. vulgaris and placebo groups (mean difference (MD) − 1·77 kg, 95 % CI − 3·33, 0·33). A further meta-analysis revealed a statistically significant reduction in body fat favouring P. vulgaris over placebo (MD − 1·86 kg, 95 % CI − 3·39, − 0·32). Heterogeneity was evident in both analyses. The poor quality of the included RCT prevents us from drawing any firm conclusions about the effects of P. vulgaris supplementation on body weight. Larger and more rigorous trials are needed to objectively assess the effects of this herbal supplement.

scholarly journals Post hoc study to investigate the potential causes of poor quality of cardiovascular medicines collected in sub-Saharan countries

BMJ Open ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. e039252
Author(s):  
Philippe-Henri Secretan ◽  
Marie Antignac ◽  
Najet Yagoubi ◽  
Mélisande Bernard ◽  
Marie Cécile Perier ◽  
...  
Keyword(s):  
Democratic Republic ◽  
Poor Quality ◽  
African Countries ◽  
Post Hoc Analysis ◽  
Significant Difference ◽  
Lack Of Control ◽  
Republic Of The Congo ◽  
Sub Saharan ◽  
Post Hoc

ObjectivesThe incidence of cardiovascular diseases is increasing and there is a growing need to provide access to quality cardio drugs in Africa. In the SEVEN study, we analysed 1530 cardiovascular drug samples randomly collected from 10 African countries. By that time, of the seven drugs products analysed, only those containing amlodipine and captopril had very low assay values with active substance contents that could be less than 75% of those expected. In this article we investigate complementary aspects of the amlodipine and captopril samples so to explain the previously observed low assays for these two drugs.DesignPost hoc analysis of the captopril and amlodipine drugs samples and their packages collected in the context of the SEVEN study.Setting10 countries were concerned: Benin, Burkina Faso, Congo, Democratic Republic of the Congo, Guinea, Côte d’Ivoire, Mauritania, Niger, Senegal and Togo.ParticipantsLocal scientists and hospital practitioners collected the drug samples in the 10 African countries.Outcome measuresThe drug amount and the relative amounts of drug impurities, as well as the main compounds of the drugs packaging, were analysed.ResultsIdentification of the blister packaging of the samples led to separate both amlodipine and captopril drug samples in two groups. Mann Whitney’s bilateral test showed a significant difference (p<0.0001) between the median value of the captopril dosage when tablets are packaged in blisters providing higher protection to humidity (n=105) as opposed to the tablets packaged in blisters providing lower humidity protection (n=130).ConclusionBased on these results, particular attention should be paid to the materials and types of packaging used in order to minimise the lack of control over the exposures and drug circuits present in these different countries.

scholarly journals Quality of Different Brands of Metronidazole Benzoate Oral Suspensions Available at Jimma Town, Southwest Ethiopia: Pharmaceuticals Quality Study

2021 ◽  
Vol 2 (3) ◽  
pp. 01-06
Author(s):  
Teshome Sosengo ◽  
Fuad Adem ◽  
Jemal Abdella
Keyword(s):  
United States ◽  
Poor Quality ◽  
Microbial Count ◽  
United States Pharmacopoeia ◽  
Drug Content ◽  
Significant Difference ◽  
Assay Result ◽  
Metronidazole Benzoate ◽  
Jimma Town

Background: Poor quality drugs include substandard and counterfeit medicines. Poor quality antibiotics selectively kill the susceptible strain and leaves resistant strain to multiply. Metronidazole is a broad spectrum antibiotic whose effectiveness depends up on a dose administered to the patient. Objective: The aim of the study is to assess the quality of different brands of metronidazole Benzoate oral suspension available in Jimma town, West Ethiopia. Methods: Cross-sectional study was conducted in Jimma town, West Ethiopia from Feb 08 – Mar 28, 2018. The assay result of all the seven brands of Metronidazole Benzoate oral suspensions was entered to statistical package for social sciences software version 24.0 for windows. Then, one way analysis of variance was performed using Tukey test to determine whether there exists significant difference in assay result of the brands (p<0.05). Result: All the seven Metronidazole benzoate oral suspensions assessed in this study passed British Pharmacopoeia 2013 specification of identity test of the drug. All the brands passed the assay test and total aerobic microbial count specification United States Pharmacopoeia 2015. The highest percentage of drug content was obtained for Metrolag, 105.56%, while the least content for Mizel, 93.12%. However, statistical comparison of drug contents at 95% confidence interval indicates that there is significant difference in drug content within and among the seven brands of Metronidazole benzoate oral suspensions (p<0.05). The pH of all the brands was with in United States Pharmacopoeia 2015 specification limit.

scholarly journals Computerized Neuropsychological Test Battery CogniSoft for Assessment of Cognition in Patients with Multiple Sclerosis

2020 ◽  
Vol 24 (4) ◽  
pp. 371-380
Author(s):  
Lyudmila Todorova ◽  
◽  
Valentina Ignatova ◽  
Jivko Surchev ◽  
◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cognitive Rehabilitation ◽  
Neuropsychological Test ◽  
Neuropsychological Testing ◽  
Poor Quality ◽  
Cognitive Status ◽  
Global Trends ◽  
Significant Difference ◽  
Overall Evaluation

Cognitive dysfunction is a leading cause of disability in multiple sclerosis (MS) and is associated with unemployment, need of assistance with daily activities and poor quality of life. The introduction of neuropsychological testing and monitoring of cognitive status as part of the overall evaluation of MS patients in parallel with clinical and paraclinical parameters is highly recommended. Recent studies have demonstrated a better perception and preference for computerized cognitive tests than classic variants, with no significant difference in results. In accordance with global trends, a bilingual computer system CogniSoft for assessment and rehabilitation of cognitive status in persons with MS has been developed, including: 1) a set of diagnostic tests for evaluation of memory and executive functions based on the nature of Brief International Cognitive Assessment for MS (BICAMS); 2) a set of games for cognitive rehabilitation. Questionnaire for depression (Beck Depression Inventory – BDI-II) will be filled before conduction of the neuropsychological tests for differentiation of possible depression which could interfere with the results. The CogniSoft information system will incorporate two approaches for evaluation of neuropsychological results which will allow early detection of cognitive impairments in these patients, which will initiate timely cognitive rehabilitation.

scholarly journals Chromosome aneuploidy analysis in embryos derived from in vivo and in vitro matured human oocytes

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Jianhua Li ◽  
Jing Chen ◽  
Tiecheng Sun ◽  
Shuiwen Zhang ◽  
Tingting Jiao ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Clinical Decision Making ◽  
Clinical Decision ◽  
Poor Quality ◽  
Vitro Fertilization ◽  
Aneuploidy Rate ◽  
Significant Difference

Abstract Background In vitro oocyte maturation (IVM) is being increasingly approached in assisted reproductive technology (ART). This study aimed to evaluate the quality of embryos generated by in-vitro matured immature follicles, as a guideline for further clinical decision-making. Methods A total of 52 couples with normal karyotypes underwent in vitro fertilization, and 162 embryos were donated for genetic screening. Embryos in IVF group were generated by mature follicles retrieved during gonadotrophin-stimulated in vitro fertilization (IVF) cycles. And embryos in IVM group were fertilized from IVM immature oocytes. Results The average age of the women was 30.50 ± 4.55 years (range 21–42 years) with 87 embryos from IVF group and 75 embryos from IVM group. The rate of aneuploid with 28 of the 87 (32.2%) embryos from IVF group and 21 of the 75 (28%) embryos from IVM group, with no significant difference. The frequency of aneuploid embryos was lowest in the youngest age and increased gradually with women’s age, whether in IVF group or IVM group and risen significantly over 35 years old. The embryos with morphological grade 1 have the lowest aneuploidy frequency (16.6%), and increase by the grade, especially in IVF group. In grade 3, embryos in IVM group were more likely to be euploid than IVF group (60% vs 40%, respectively). Conclusions IVM does not affect the quality of embryos and does not increase the aneuploidy rate of embryos. It is clinically recommended that women more than 35 years have a high aneuploidy rate and recommended to test by PGS (strongly recommended to screened by PGS for women more than 40 years). Women aged less than 35 years old for PGS according to their physical and economic conditions. Embryo with poor quality is also recommended to test by PGS, especially for grade III embryos.

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