scholarly journals Screening of biological activity of phytopreparations based on secondary metabolites of plants on the culture of Phaseolus vulgaris

2021 ◽  
pp. 89-97
Author(s):  
E. G. Kozar ◽  
I. A. Engalycheva ◽  
A. A. Antoshkin ◽  
N. E. Mashcenco
Keyword(s):  
Biological Activity ◽  
Plant Protection ◽  
Physiological Activity ◽  
Control Treatment ◽  
Seed Productivity ◽  
High Biological Activity ◽  
Disease Etiology ◽  
Total Percentage ◽  
Biological Effectiveness ◽  
Seed Crops

Relevance. Currently, the use of secondary plant-orgin metabolites with high biological activity is becoming an important part of a comprehensive plant protection program. The mechanism of their action is aimed at activating molecular biological and physiological systems that increase the ability of the plant organism to withstand the adverse effects of biotic and abiotic nature. The purpose of these studies is to screen the selectivity of the physiological activity of phytoregulators in relation to different varieties of vegetable beans.Methodology. Plants of two mid-ripening varieties of vegetable beans were twice sprayed with solutions of plant biological products of different chemical composition, isolated from 12 plant species of different families. Control – treatment with water, standards - solutions of Pharmayod and Fitolavin. During the growing season, an analysis of the development of diseases of various etiologies on seed crops was carried out. Seed productivity of plants was taken into account after harvesting and threshing the beans, the sowing qualities of the obtained seeds were studied in accordance with the relevant recommendations and GOST 12042-80, and the biological effectiveness (BE) of the action of phytopreparations was calculated.Results. Two-fold treatment of beans with most of the tested preparations reduced the total percentage of affected plants by more than 25%. The protective effect of the studied glycosides differed significantly depending on their chemical structure, disease etiology and cultivar responsiveness. Among them, there are preparations based on steroidal glycosides - Moldstim and Mestim, which have the most pronounced immunomodulatory effect on the resistance of vegetable beans to phytopathogens. Treatment with Mestim in the selected concentration led to a decrease in seed productivity of plants. Other phytopreparations showed a neutral or stimulating effect on this trait, increasing the proportion of germinating seeds in the yield structure by 3-26% relative to control. The certain varietal specificity has been identified by the total effectiveness of the action. Irioid glycosides from Linaria genistifolia and the sum of iridoids and flavonoids from Linaria vulgaris showed the greatest activity on the responsive cultivar Lika. On the SiBemol variety, there is Moldstim, a steroidal glycoside of the furostanol series, isolated from Capsicum annuum. Their biological effectiveness was higher than that of Fitolavin and Pharmayod standards.

scholarly journals Influence of vegetation treatment with growth stimulants and protection agents on seed productivity of meadow fescue Nadezhda

2020 ◽  
Vol 202 (11) ◽  
pp. 28-36
Author(s):  
M TORMOZIN ◽  
A. Belyaev ◽  
E. Tiholaz
Keyword(s):  
Plant Protection ◽  
Growing Season ◽  
Plant Protection Products ◽  
Seed Productivity ◽  
Meadow Fescue ◽  
Hydrothermal Coefficient ◽  
Yield Structure ◽  
Biological Effectiveness ◽  
Reliable Increase

Abstract. The article provides information about the yield, development of leaf-stem diseases, as well as the biological effectiveness of drugs on meadow fescue crops. The purpose of research is to study the influence of protective agents and growth stimulants that provide the most complete realization of the potential of seed productivity of meadow fescue. After applying protection products, crop structure for 2018–2019 Treatment of plants for vegetation in the phase of entering the tube – the beginning of earing, the following drugs were used: “Fitolavin” – 1.5 l/ha; “Lariksin” – 50 g/ha; “Kolosal’ Pro” – 0.5 l/ha; “Strekar” – 1.5 l/ha; “Fitolavin” – 1.5 l/ha + “Kolosal’ Pro” – 0.5 l/ha. Research methodology and methods. The experience consists of 18 plots. The total area of one plot is 42 m2, the accounting area of one plot is 28 m2. Plots are placed – blockwise, repetitions are rendomized, the repetition is 3-fold. Observations and studies were conducted according to the generally accepted methodology. Meteorological conditions in 2018–2019 significantly varied from the long-term average. The hydrothermal coefficient (GTC) for the growing season in 2018 was 1.4; in 2019, it was 1.77, which is higher than the long – term average (1.57). Results. The development of the disease on average for all tiers of leaves for two years (2018–2019) in the control was 23.7 %, the prevalence – 67.5 %. In the variants with fungicides and biologics, the development and prevalence of leaf-stem diseases ranged from 9.4 to 17.4 % and from 32.4 to 56.5 %, respectively. The use of plant protection products led to a decrease in the development of diseases by 1.4–2.5 times (by 6.3–14.3 %), and the prevalence – by 1.2–2.1 times (by 11.0–35.1 %).The biological effectiveness of plant protection products ranged from 26.6 to 60.3 %. The applied preparations on average for two years of research provided a reliable increase in seed yield from 0.11 to 0.26 t/ha (from 32 to 76 %). Scientific novelty. The increase in seed productivity according to two-year data (2018–2019) was obtained due to a significant increase in all components of the yield structure.

A FACILE SYNTHESIS AND REACTIONS OF AMINO SELENOLO[2,3-b]PYRIDINE CARBOXYLATE

2015 ◽  
Vol 12 (1) ◽  
pp. 3910-3918 ◽  
Author(s):  
Dr Remon M Zaki ◽  
Prof Adel M. Kamal El-Dean ◽  
Dr Nermin A Marzouk ◽  
Prof Jehan A Micky ◽  
Mrs Rasha H Ahmed
Keyword(s):  
Biological Activity ◽  
Carbonyl Compounds ◽  
Mass Spectra ◽  
The Other ◽  
Pyridine Derivatives ◽  
Facile Synthesis ◽  
High Biological Activity ◽  
Basic Hydrolysis ◽  
Pyridine Carboxylate ◽  
Hydrolysis Of

 Incorporating selenium metal bonded to the pyridine nucleus was achieved by the reaction of selenium metal with 2-chloropyridine carbonitrile 1 in the presence of sodium borohydride as reducing agent. The resulting non isolated selanyl sodium salt was subjected to react with various α-halogenated carbonyl compounds to afford the selenyl pyridine derivatives 3a-f  which compounds 3a-d underwent Thorpe-Ziegler cyclization to give 1-amino-2-substitutedselenolo[2,3-b]pyridine compounds 4a-d, while the other compounds 3e,f failed to be cyclized. Basic hydrolysis of amino selenolo[2,3-b]pyridine carboxylate 4a followed by decarboxylation furnished the corresponding amino selenolopyridine compound 6 which was used as a versatile precursor for synthesis of other heterocyclic compound 7-16. All the newly synthesized compounds were established by elemental and spectral analysis (IR, 1H NMR) in addition to mass spectra for some of them hoping these compounds afforded high biological activity.

scholarly journals The Psychonauts’ Benzodiazepines; Quantitative Structure-Activity Relationship (QSAR) Analysis and Docking Prediction of Their Biological Activity

2021 ◽  
Vol 14 (8) ◽  
pp. 720
Author(s):  
Valeria Catalani ◽  
Michelle Botha ◽  
John Martin Corkery ◽  
Amira Guirguis ◽  
Alessandro Vento ◽  
...  
Keyword(s):  
Biological Activity ◽  
Computational Models ◽  
Quantitative Structure Activity Relationship ◽  
Structure Activity Relationship ◽  
Docking Studies ◽  
Health Concern ◽  
Activity Relationship ◽  
Quantitative Structure ◽  
High Biological Activity ◽  
Structure Activity

Designer benzodiazepines (DBZDs) represent a serious health concern and are increasingly reported in polydrug consumption-related fatalities. When new DBZDs are identified, very limited information is available on their pharmacodynamics. Here, computational models (i.e., quantitative structure-activity relationship/QSAR and Molecular Docking) were used to analyse DBZDs identified online by an automated web crawler (NPSfinder®) and to predict their possible activity/affinity on the gamma-aminobutyric acid A receptors (GABA-ARs). The computational software MOE was used to calculate 2D QSAR models, perform docking studies on crystallised GABA-A receptors (6HUO, 6HUP) and generate pharmacophore queries from the docking conformational results. 101 DBZDs were identified online by NPSfinder®. The validated QSAR model predicted high biological activity values for 41% of these DBDZs. These predictions were supported by the docking studies (good binding affinity) and the pharmacophore modelling confirmed the importance of the presence and location of hydrophobic and polar functions identified by QSAR. This study confirms once again the importance of web-based analysis in the assessment of drug scenarios (DBZDs), and how computational models could be used to acquire fast and reliable information on biological activity for index novel DBZDs, as preliminary data for further investigations.

Replacement of the interchain disulfide bridge-forming amino acids A7 and B7 by glutamate impairs the structure and activity of insulin

2004 ◽  
Vol 385 (12) ◽  
pp. 1171-1175 ◽  
Author(s):  
Zhan-Yun Guo ◽  
Xiao-Yuan Jia ◽  
You-Min Feng
Keyword(s):  
Biological Activity ◽  
Disulfide Bonds ◽  
Negative Charge ◽  
Disulfide Bridge ◽  
Site Directed Mutagenesis ◽  
Side Chain ◽  
Insulin Analogs ◽  
High Biological Activity ◽  
Chemical Groups ◽  
Structure And Activity

Abstract Insulin contains three disulfide bonds, one intrachain bond, A6–A11, and two interchain bonds, A7–B7 and A20–B19. Site-directed mutagenesis results (the two cysteine residues of disulfide A7–B7 were replaced by serine) showed that disulfide A7–B7 is crucial to both the structure and activity of insulin. However, chemical modification results showed that the insulin analogs still retained relatively high biological activity when A7Cys and B7Cys were modified by chemical groups with a negative charge. Did the negative charge of the modification groups restore the loss of activity and/or the disturbance of structure of these insulin analogs caused by deletion of disulfide A7–B7? To answer this question, an insulin analog with both A7Cys and B7Cys replaced by Glu, which has a long side-chain and a negative charge, was prepared by protein engineering, and its structure and activity were analyzed. Both the structure and activity of the present analog are very similar to that of the mutant with disulfide A7–B7 replaced by Ser, but significantly different from that of wild-type insulin. The present results suggest that removal of disulfide A7–B7 will result in serious loss of biological activity and the native conformation of insulin, even if the disulfide is replaced by residues with a negative charge.

scholarly journals Extraction Techniques and Analytical Methods for Characterization of Active Compounds in Origanum Species

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4735
Author(s):  
Maša Knez Hrnčič ◽  
Darija Cör ◽  
Jana Simonovska ◽  
Željko Knez ◽  
Zoran Kavrakovski ◽  
...  
Keyword(s):  
Biological Activity ◽  
Abiotic Stresses ◽  
Plant Protection ◽  
Analytical Techniques ◽  
Extraction Techniques ◽  
Isolation And Identification ◽  
Activity Assessment ◽  
Current Contribution ◽  
Pharmaceutical Industries

Phytochemical research based on ethnopharmacology is gaining interest in industries such as functional food, nutraceuticals, cosmetics and pharmaceutical industries. Plants and plant extracts are a rich source of bioactive secondary metabolites. These compounds are often involved in plant protection against biotic or abiotic stresses. The exploitation of available technologies should be oriented and intensified to extend and enhance the continued usefulness of the plants as renewable sources of chemicals, especially medicinal compounds. This current contribution is focused on extraction and analytical techniques for their isolation from the oregano species, their characterization and their potential antioxidative, as well as their antimicrobial, antifungal and anticarcinogenic properties. The work is structured rendering to the different steps involved in the research; starting with extraction and sample preparation, followed by discussing the analytical techniques employed for the isolation and identification of compound/s responsible for the biological activity and methods and techniques for biological activity assessment.

scholarly journals Molecular Evolution Allows Bypass of the Requirement for Activation Loop Phosphorylation of the Cdc28 Cyclin-Dependent Kinase

1998 ◽  
Vol 18 (5) ◽  
pp. 2923-2931 ◽  
Author(s):  
Frederick R. Cross ◽  
Kristi Levine
Keyword(s):  
Cell Cycle ◽  
Biological Activity ◽  
Cell Cycle Progression ◽  
Dna Amplification ◽  
Protein Kinase Activity ◽  
Activation Loop ◽  
Cyclin Dependent Kinase ◽  
High Biological Activity ◽  
Cycle Progression ◽  
Growth Defects

ABSTRACT Many protein kinases are regulated by phosphorylation in the activation loop, which is required for enzymatic activity. Glutamic acid can substitute for phosphothreonine in some proteins activated by phosphorylation, but this substitution (T169E) at the site of activation loop phosphorylation in the Saccharomyces cerevisiae cyclin-dependent kinase (Cdk) Cdc28p blocks biological function and protein kinase activity. Using cycles of error-prone DNA amplification followed by selection for successively higher levels of function, we identified mutant versions of Cdc28p-T169E with high biological activity. The enzymatic and biological activity of the mutant Cdc28p was essentially normally regulated by cyclin, and the mutants supported normal cell cycle progression and regulation. Therefore, it is not a requirement for control of the yeast cell cycle that Cdc28p be cyclically phosphorylated and dephosphorylated. TheseCDC28 mutants allow viability in the absence of Cak1p, the essential kinase that phosphorylates Cdc28p-T169, demonstrating that T169 phosphorylation is the only essential function of Cak1p. Some growth defects remain in suppressed cak1 cdc28 strains carrying the mutant CDC28 genes, consistent with additional nonessential roles for CAK1.

scholarly journals Analysis of thiram derivatives use in plant complex of the Kursk region

2020 ◽  
Vol 28 (2) ◽  
pp. 103-111
Author(s):  
Vladimir A. Korolev ◽  
Olga A. Medvedeva ◽  
Vera A. Ryadnova ◽  
Sergey A. Losenok ◽  
Ekaterina S. Nikitina ◽  
...  
Keyword(s):  
Human Body ◽  
Environmental Changes ◽  
Plant Protection ◽  
Modern Human ◽  
Anthropogenic Factors ◽  
Industrial Complex ◽  
Tetramethylthiuram Disulfide ◽  
Seed Crops ◽  
The Impact ◽  
Kursk Region

The impact on the biosphere of anthropogenic factors is a serious environmental problem. Today, pesticides are becoming one of the important chemical factors that cause adverse environmental changes. Pesticides enter the human body through a contaminated hydrosphere, atmosphere, and food. Modern man is not able to grow and maintain crops without the use of pesticides. Penetrating into the human body, they cumulate, thereby contributing to the creation of threatening health situations. Despite this, modern human civilization cannot do without them. The purpose of the review was to study the circulation volume of derivatives of thiram, such as vitalon, vitavaks, vitasil, vitaros, tir and TMTD (Tetramethylthiuram Disulfide) in the agricultural complex of the Kursk region for the period 2007-2016. The data obtained at the Kursk Regional Plant Protection Station, as well as agrochemical protection station Kurskaya, were subjected to statistical analysis using the application Statistica 6.0. Circulating volumes of thiram's derivatives were examined in agro-industrial complex of Kursk area over a 10-year period. The proportion of fungicides of thiram's series with the release of agrochemicals dominant in the structure of seed crops cultivation technologies was investigated. A study of the structure of sown areas with the allocation of priority crops grown in the region was conducted, the total number of which amounted 80.76%. The ranking of the region by thirams derivatives was made. All studied fungicidal products of thiram are widely used for the cultivation of crops in the Kursk region. Identified regions with the biggest amount of making this group of agrochemicals in the soil are treated as high anthropogenic load zone and require the development of environmental protection. The volume of circulation of the studying group of pesticides can be dangerous to humans and animals, influence the formation of peoples somatic pathologies.

scholarly journals INFLUENCE OF BIOLOGICAL PROTECTION OF PLANTS ON SOIL MYCOCENOSIS WHEN GROWING LEAF SALAD UNDER ARID CLIMATE OF ASTRAKHAN REGION

ÈKOBIOTEH ◽  
2021 ◽  
Vol 4 (3) ◽  
pp. 221-226
Author(s):  
D.G. Baubekova ◽  
Keyword(s):  
Biological Activity ◽  
Drip Irrigation ◽  
Plant Protection ◽  
Growing Season ◽  
Arid Climate ◽  
Phytopathogenic Bacteria ◽  
Agent Based ◽  
Air Temperatures ◽  
Biological Protection ◽  
Protection Agent

Territories with arid climate are characterized by insufficient atmospheric humidification at high air temperatures. Therefore, the use of drip irrigation during the growing season of plants is an effective agrotechnical method of farming. However, its use causes a number of unfavorable consequences that can be avoided by using biological plant protection agents with pronounced biological activity against phytopathogenic bacteria, micromycetes, insects and viruses. These remedies are based on the introduction of microorganisms and their metabolites into the biotechnological process. A biological plant protection agent based on Bаcillus atrophaeus ACIM В–11474 has been developed and its effect on the mycocenosis of the studied soils when growing lettuce in the arid climate of the Astrakhan region using drip irrigation has been studied.

INFLUENCE OF INSECTICIDES ON PRODUCTIVITY IN RAPE CROPS IN AKMOLA REGION

2021 ◽  
Author(s):  
М.Н.Сертек,Ш.Ш. Бекенова,А.П.Науанова, З.Ш Сулейменова
Keyword(s):  
High Efficiency ◽  
Plant Protection ◽  
Growing Season ◽  
Plant Protection Products ◽  
Steppe Zone ◽  
Climatic Conditions ◽  
Ordinary Chernozem ◽  
Forest Steppe ◽  
Biological Effectiveness ◽  
Harmful Organisms

According to the results of research on the phytosanitary status of rapeseed on ordinary Chernozem of the forest-steppe zone of the Akmola region, the species composition of pests was determined. Danadim power, Zolone 35% KE, Decis Expert were used against pests of this rapeseed. Indicators of biological effectiveness in the use of insecticides against pests ranged from 78-95%.The article discusses the biological and economic effectiveness of insecticides used against the main pests of rapeseed. Treatment of cruciferous rape beetle, rapeseed sawfly and cruciferous bug with Decis Expert insecticide (0.075 l/ha) showed high efficiency during the growing season. The use of plant protection products against a complex of harmful organisms showed that, depending on the prevailing climatic conditions, the yield increased to 1.3 c/ha, and the maximum - to 1.9 c/ha. Thus, according to the biological effectiveness of insecticides used against pests, the productDecis Expert showed high results in all pests. On the Cruciferous rape beetle it was 85.7%, in the turnip sawfly – 93.5%, in the cruciferous bug – 81.9%.

NON-MEDICAL ANTIBIOTIC IMBRICIN –ECOLOGICALLY SAFE PESTICIDE FOR PLANT PROTECTION

2021 ◽  
Vol 1 (19) ◽  
pp. 361-363
Author(s):  
I.V. Boykova ◽  
O.V. Topkova ◽  
O.I. Korotkova
Keyword(s):  
Biological Activity ◽  
Plant Protection ◽  
Biological Product

The biological activity of the non-medical antibiotic imbricin was studied in order to determine the possibility of its use as an ecologically safe and effective biological product for protecting plants from diseases and harmful arthropods.

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