Immune physiology in tissue regeneration and aging, tumor growth, and regenerative medicine

As a technique of producing fabric engineering scaffolds, three-dimensional (3D) printing has tremendous possibilities. 3D printing applications are restricted to a wide range of biomaterials in the field of regenerative medicine and tissue engineering. Due to their biocompatibility, bioactiveness, and biodegradability, biopolymers such as collagen, alginate, silk fibroin, chitosan, alginate, cellulose, and starch are used in a variety of fields, including the food, biomedical, regeneration, agriculture, packaging, and pharmaceutical industries. The benefits of producing 3D-printed scaffolds are many, including the capacity to produce complicated geometries, porosity, and multicell coculture and to take growth factors into account. In particular, the additional production of biopolymers offers new options to produce 3D structures and materials with specialised patterns and properties. In the realm of tissue engineering and regenerative medicine (TERM), important progress has been accomplished; now, several state-of-the-art techniques are used to produce porous scaffolds for organ or tissue regeneration to be suited for tissue technology. Natural biopolymeric materials are often better suited for designing and manufacturing healing equipment than temporary implants and tissue regeneration materials owing to its appropriate properties and biocompatibility. The review focuses on the additive manufacturing of biopolymers with significant changes, advancements, trends, and developments in regenerative medicine and tissue engineering with potential applications.

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Synthesis and characterization of a photocrosslinkable chitosan–gelatin hydrogel aimed for tissue regeneration

RSC Advances ◽

10.1039/c5ra10638a ◽

2015 ◽

Vol 5 (78) ◽

pp. 63478-63488 ◽

Cited By ~ 36

Author(s):

Sofia M. Saraiva ◽

Sónia P. Miguel ◽

Maximiano P. Ribeiro ◽

Paula Coutinho ◽

Ilídio J. Correia

Keyword(s):

Tissue Engineering ◽

Regenerative Medicine ◽

Tissue Regeneration ◽

Synthesis And Characterization ◽

Native Structure ◽

Engineering Applications ◽

Gelatin Hydrogel

In the area of regenerative medicine different approaches have been studied to restore the native structure of damaged tissues. Herein, the suitability of a photocrosslinkable hydrogel for tissue engineering applications was studied.

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The biochemical determinants of tissue regeneration

Biochemical Society Transactions ◽

10.1042/bst20140095 ◽

2014 ◽

Vol 42 (3) ◽

pp. 607-608

Author(s):

Adam Giangreco ◽

Catherine L.R. Merry

Keyword(s):

Regenerative Medicine ◽

Tissue Regeneration ◽

Normal Tissue ◽

Healthcare Research ◽

Human Cells ◽

Annual Symposium ◽

Society Annual ◽

Living Materials ◽

Research Tools

The field of regenerative medicine offers tantalizing hope for the repair and replacement of damaged organs and tissues, with the ultimate goal of restoring normal tissue function. This field represents an enormous range of biological, chemical and biophysical technologies that harness the restorative properties of living materials, especially human cells, to produce new molecular and cellular medicines, diagnostics, devices and healthcare research tools. The goal of this Biochemical Society Annual Symposium was to explore the key biochemical determinants of tissue regeneration, and we highlight the contribution of biochemistry to this emerging field of regenerative medicine.

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ID:3006 RNA Therapeutics and Anabolic Gene Delivery for Tissue Regeneration

Biomedical Research and Therapy ◽

10.15419/bmrat.v4is.224 ◽

2017 ◽

Vol 4 (S) ◽

pp. 18

Author(s):

Yu-Chen Hu

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Gene Delivery ◽

Regenerative Medicine ◽

Tissue Regeneration ◽

Viral Vectors ◽

Gene Delivery Vector ◽

Delivery Vector ◽

Genes Encoding

Regenerative medicine requires coordinated functions of cells, materials and appropriate signaling. Recent years have witnessed the marriage of regenerative medicine and gene delivery by which various genes encoding anabolic/catabolic proteins or RNA therapeutics are delivered into cells to potentiate the tissue regeneration. This presentation will focus on the use of viral vectors for genetic modification of mesenchymal stem cells derived from bone marrow or adipose tissue for tissue regeneration. In particular, emphasis is placed on the applications of baculovirus, an emerging nonpathogenic gene delivery vector, for the delivery of various anabolic genes and miRNA mimics/sponges to repair tissues

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M1-like macrophage contributes to chondrogenesis in vitro

Scientific Reports ◽

10.1038/s41598-021-00232-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yoshiyuki Miyamoto ◽

Keigo Kubota ◽

Yukiyo Asawa ◽

Kazuto Hoshi ◽

Atsuhiko Hikita

Keyword(s):

Regenerative Medicine ◽

Tissue Regeneration ◽

Tissue Damage ◽

Collagen Type ◽

Experimental System ◽

Cartilage Tissue ◽

Oral And Maxillofacial Regions

AbstractCartilage tissues have poor self-repairing abilities. Regenerative medicine can be applied to recover cartilage tissue damage in the oral and maxillofacial regions. However, hitherto it has not been possible to predict the maturity of the tissue construction after transplantation or to prepare mature cartilage tissues before transplantation that can meet clinical needs. Macrophages play an important role in cartilage tissue regeneration, although the exact mechanisms remain unknown. In this study, we established and verified an in vitro experimental system for the direct co-culture of cell pellets prepared from mouse auricular chondrocytes and macrophages polarized into four phenotypes (M1-like, M1, M2-like, and M2). We demonstrate that cartilage pellets co-cultured with M1-like promoted collagen type 2 and aggrecan production and induced the most significant increase in chondrogenesis. Furthermore, M1-like shifted to M2 on day 7 of co-culture, suggesting that the cartilage pellet supplied factors that changed the polarization of M1-like. Our findings suggest that cartilage regenerative medicine will be most effective if the maturation of cartilage tissues is induced in vitro by co-culture with M1-like before transplantation.

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Hydrogel Scaffolds - Emerging Biomaterial for Regenerative Medicine

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2016.9.2.2 ◽

1970 ◽

Vol 9 (2) ◽

pp. 3156-3168

Author(s):

Prajeesh Kumar ◽

Shivansh Swamy ◽

Raj K. Narang

Keyword(s):

Tissue Engineering ◽

Growth Factors ◽

Regenerative Medicine ◽

Tissue Regeneration ◽

Cellular Proliferation ◽

Hydrogel Scaffold ◽

Hydrogel Scaffolds ◽

Cellular Environment ◽

Direct Incorporation ◽

Unique Ability

The regenerative medicine field has led to the development of various biomaterials. One such development is in the form of Hydrogel Scaffold for Tissue regeneration. This review describes the biomedical advances in Hydrogel Scaffolds as emerging biomaterial for regenerative medicine. Their unique ability to mimic the extra cellular environment, biocompatibility, flexible method of synthesis, desirable framework for cellular proliferation and survival has made them the material of choice. Hydrogels have demonstrated features which exemplify many of the broad-based manifestations of tissue engineering, providing realized as well as potential commercial value. Direct incorporation of cells and growth factors has led to efficient and more promising results in regenerative medicine. This review gives an overview of the various kinds of hydrogels, fabrication methods with specific features and few of the recent applications of hydrogels in the field of regenerative medicine.

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Proteins and Small Molecules for Cellular Regenerative Medicine

Physiological Reviews ◽

10.1152/physrev.00005.2012 ◽

2013 ◽

Vol 93 (1) ◽

pp. 311-325 ◽

Cited By ~ 19

Author(s):

Eric M. Green ◽

Richard T. Lee

Keyword(s):

Regenerative Medicine ◽

Small Molecules ◽

Tissue Regeneration ◽

Target Cell ◽

Building Blocks ◽

Cell Type ◽

Multipotent Cells ◽

Fundamental Goal ◽

Treatment Paradigm ◽

Ischemic Diseases

Regenerative medicine seeks to understand tissue development and homeostasis and build on that knowledge to enhance regeneration of injured tissues. By replenishing lost functional tissues and cells, regenerative medicine could change the treatment paradigm for a broad range of degenerative and ischemic diseases. Multipotent cells hold promise as potential building blocks for regenerating lost tissues, but successful tissue regeneration will depend on comprehensive control of multipotent cells–differentiation into a target cell type, delivery to a desired tissue, and integration into a durable functional structure. At each step of this process, proteins and small molecules provide essential signals and, in some cases, may themselves act as effective therapies. Identifying these signals is thus a fundamental goal of regenerative medicine. In this review we discuss current progress using proteins and small molecules to regulate tissue regeneration, both in combination with cellular therapies and as monotherapy.

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Extracellular vesicles, exosomes and shedding vesicles in regenerative medicine – a new paradigm for tissue repair

Biomaterials Science ◽

10.1039/c7bm00479f ◽

2018 ◽

Vol 6 (1) ◽

pp. 60-78 ◽

Cited By ~ 83

Author(s):

I. M. Bjørge ◽

S. Y. Kim ◽

J. F. Mano ◽

B. Kalionis ◽

W. Chrzanowski

Keyword(s):

Regenerative Medicine ◽

Extracellular Vesicles ◽

Tissue Regeneration ◽

Tissue Repair ◽

New Paradigm ◽

Ribonucleic Acids ◽

Biological Signals

Extracellular vesicles are highly specialized messengers that deliver vital biological signals including ribonucleic acids – key modulators in tissue regeneration.

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Types of immune-inflammatory responses as a reflection of cell–cell interactions under conditions of tissue regeneration and tumor growth

Biochemistry (Moscow) ◽

10.1134/s0006297917050029 ◽

2017 ◽

Vol 82 (5) ◽

pp. 542-555 ◽

Cited By ~ 7

Author(s):

L. A. Tashireva ◽

V. M. Perelmuter ◽

V. N. Manskikh ◽

E. V. Denisov ◽

O. E. Savelieva ◽

...

Keyword(s):

Tumor Growth ◽

Tissue Regeneration ◽

Inflammatory Responses ◽

Cell Interactions ◽

Cell Cell

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Wound Healing Versus Regeneration: Role of the Tissue Environment in Regenerative Medicine

MRS Bulletin ◽

10.1557/mrs2010.528 ◽

2010 ◽

Vol 35 (8) ◽

pp. 597-606 ◽

Cited By ~ 39

Author(s):

Anthony Atala ◽

Darrell J. Irvine ◽

Marsha Moses ◽

Sunil Shaunak

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Regenerative Medicine ◽

Tissue Regeneration ◽

Normal Tissue ◽

Disease Process ◽

Scar Formation ◽

The Body ◽

Mechanistic Basis

AbstractOne of the major challenges in the field of regenerative medicine is how to optimize tissue regeneration in the body by therapeutically manipulating its natural ability to form scar at the time of injury or disease. It is often the balance between tissue regeneration, a process that is activated at the onset of disease, and scar formation, which develops as a result of the disease process that determines the ability of the tissue or organ to be functional. Using biomaterials as scaffolds often can provide a “bridge” for normal tissue edges to regenerate over small distances, usually up to 1 cm. Larger tissue defect gaps typically require both scaffolds and cells for normal tissue regeneration to occur without scar formation. Various strategies can help to modulate the scar response and can potentially enhance tissue regeneration. Understanding the mechanistic basis of such multivariate interactions as the scar microenvironment, the immune system, extracellular matrix, and inflammatory cytokines may enable the design of tissue engineering and wound healing strategies that directly modulate the healing response in a manner favorable to regeneration.

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