scholarly journals Common pine (Pinus sylvestris L.) pollen quality in JSC «Karabashmed» emission zone

2021 ◽  
Vol 25 (1) ◽  
pp. 32-44
Author(s):  
S.G. Makhniova ◽  
◽  
S.L. Menshchikov ◽  
◽  
Keyword(s):  
High Frequency ◽  
Male Gametophyte ◽  
Pollen Grains ◽  
Technogenic Pollution ◽  
Significant Similarity ◽  
Study Results ◽  
Pine Pollen ◽  
Negative Effect ◽  
Pine Stands ◽  
Generative System

The study results of Common pine mature pollen state in the smoke emissions from the plant JSC «Karabashmed» in connection with the level of technogenic impact are presented. It is shown that the state of the vegetation cover is in accordance with the level of technogenic pollution, determined by the accumulation degree of air pollutants in the snow cover. It was established that the soil and vegetation were degraded in the flare zone of the plant. There are no pine stands at a distance of less than 3.5 km from the source of smoke emissions. Symptoms of chronic and acute damage to the assimilation apparatus and disturbance of the generative sphere of pine at a distance of 8 km to the southeast were revealed. A significant similarity in the vital state of pine stands at a distance of 13 km (zone of low aerosol pollution) and 19 km (background conditions) from the source was established. At the same time, the frequency of small and degenerated pollen was many times higher in the pool of mature pine pollen in the stand under conditions of a low level of pollution than under background conditions. A high frequency of pollen grains with anomalies of air pockets was detected in the stand under background conditions. The results of the study indicate a high susceptability of the male generative system to technogenic pollution (accumulated soil, aerosol, gas) and the possibility of its bioindication in the absence of symptoms of damage to the assimilation apparatus. It was shown that disturbances in the development of male gametophyte in the zones of technogenic pollution are already realized at the early stages of microspore development and are revealed by the high frequency of small underdeveloped pollen. It was found that most of the pine trees growing on the soil of fine-stone-sandy composition correspond to the categories of weakened and dying ones, which makes it possible to recognize the conditions of the man-made land as extremely unfavorable for the growth and development of pine. The mature pine pollen of these stands is characterized by the peculiar features that distinguish it from the pollen of other stands. The conclusion is made about the negative effect of smoke emissions from the JSC «Karabashmed» plant on the condition of pine plantations and the male generative system of pine, even at a considerable distance from the source of emissions.

scholarly journals Evaluation of numerical models by FerryBox and fixed platform in situ data in the southern North Sea

Ocean Science ◽  
2015 ◽  
Vol 11 (6) ◽  
pp. 879-896 ◽  
Author(s):  
M. Haller ◽  
F. Janssen ◽  
J. Siddorn ◽  
W. Petersen ◽  
S. Dick
Keyword(s):  
North Sea ◽  
High Frequency ◽  
Numerical Models ◽  
Spatial Scales ◽  
Model Performance ◽  
Eastern Coast ◽  
Southern North Sea ◽  
In Situ Data ◽  
Oyster Ground ◽  
Study Results

Abstract. For understanding and forecasting of hydrodynamics in coastal regions, numerical models have served as an important tool for many years. In order to assess the model performance, we compared simulations to observational data of water temperature and salinity. Observations were available from FerryBox transects in the southern North Sea and, additionally, from a fixed platform of the MARNET network. More detailed analyses have been made at three different stations, located off the English eastern coast, at the Oyster Ground and in the German Bight. FerryBoxes installed on ships of opportunity (SoO) provide high-frequency surface measurements along selected tracks on a regular basis. The results of two operational hydrodynamic models have been evaluated for two different time periods: BSHcmod v4 (January 2009 to April 2012) and FOAM AMM7 NEMO (April 2011 to April 2012). While they adequately simulate temperature, both models underestimate salinity, especially near the coast in the southern North Sea. Statistical errors differ between the two models and between the measured parameters. The root mean square error (RMSE) of water temperatures amounts to 0.72 °C (BSHcmod v4) and 0.44 °C (AMM7), while for salinity the performance of BSHcmod is slightly better (0.68 compared to 1.1). The study results reveal weaknesses in both models, in terms of variability, absolute levels and limited spatial resolution. Simulation of the transition zone between the coasts and the open sea is still a demanding task for operational modelling. Thus, FerryBox data, combined with other observations with differing temporal and spatial scales, can serve as an invaluable tool not only for model evaluation, but also for model optimization by assimilation of such high-frequency observations.

scholarly journals Transformation of Seed Non-Transmissible Hop Viroids in Nicotiana benthamiana Causes Distortions in Male Gametophyte Development

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2398
Author(s):  
Lenka Steinbachová ◽  
Jaroslav Matoušek ◽  
Gerhard Steger ◽  
Helena Matoušková ◽  
Sebastjan Radišek ◽  
...  
Keyword(s):  
Nicotiana Benthamiana ◽  
Male Gametophyte ◽  
Pollen Grains ◽  
Potato Spindle Tuber Viroid ◽  
Apple Fruit ◽  
Mature Pollen ◽  
Rare Occurrence ◽  
Gametophyte Development ◽  
Male Gametophyte Development ◽  
Marker Proteins

Viroids are small, non-coding, parasitic RNAs that promote developmental distortions in sensitive plants. We analyzed pollen of Nicotiana benthamiana after infection and/or ectopic transformation with cDNAs of citrus bark cracking viroid (CBCVd), apple fruit crinkle viroid (AFCVd) and potato spindle tuber viroid (PSTVd) variant AS1. These viroids were seed non-transmissible in N. benthamiana. All viroids propagated to high levels in immature anthers similar to leaves, while their levels were drastically reduced by approximately 3.6 × 103, 800 and 59 times in mature pollen of CBCVd, AFCVd and PSTVd infected N. benthamiana, respectively, in comparison to leaves. These results suggest similar elimination processes during male gametophyte development as in the Nicotiana tabacum we presented in our previous study. Mature pollen of N. benthamiana showed no apparent defects in infected plants although all three viroids induced strong pathological symptoms on leaves. While Nicotiana species have naturally bicellular mature pollen, we noted a rare occurrence of mature pollen with three nuclei in CBCVd-infected N. benthamiana. Changes in the expression of ribosomal marker proteins in AFCVd-infected pollen were detected, suggesting some changes in pollen metabolism. N. benthamiana transformed with 35S-driven viroid cDNAs showed strong symptoms including defects in pollen development. A large number of aborted pollen (34% and 62%) and a slight increase of young pollen grains (8% and 15%) were found in mature pollen of AFCVd and CBCVd transformants, respectively, in comparison to control plants (3.9% aborted pollen and 0.3% young pollen). Moreover, pollen grains with malformed nuclei or trinuclear pollen were found in CBCVd-transformed plants. Our results suggest that “forcing” overexpression of seed non-transmissible viroid led to strong pollen pathogenesis. Viroid adaptation to pollen metabolism can be assumed as an important factor for viroid transmissibility through pollen and seeds.

scholarly journals PHYSIOLOGICAL STATE AND PRODUCTIVITY OF ANIMALS UNDER THERMAL STRESS

2019 ◽  
Vol 51 (3) ◽  
pp. 26-31
Author(s):  
E.A. Azhmuldinov ◽  
◽  
Yu.N. Chernyshenko ◽  
M.G. Titov ◽  
◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Physiological State ◽  
Live Weight ◽  
Blood Parameters ◽  
Maximum Temperature ◽  
Total Plasma ◽  
Ambient Temperatures ◽  
Study Results ◽  
Total Plasma Protein ◽  
Negative Effect

A study was conducted of the effect of heat stress on the clinical and physiological state of male rabbits at the age of 5 months with an average live weight of 1551 g. The maximum temperature the animals were exposed to during the experiment was +42 °C. The study results showed a decrease in feed intake and an increase in water consumption, which contributed to a decrease in live weight. Hematological parameters, including hemoglobin, erythrocytes, leukocytes, total plasma protein, globulins and cholesterol increased during the action of this stress factor. It was found that the effect of high ambient temperatures on rabbits negatively affected their physiological functions; the negative effect was reflected by the morphological and biochemical blood parameters.

scholarly journals Ecosystem Services and Disservices of Vegetation in Recreational Urban Blue-Green Spaces—Some Recommendations for Greenery Shaping

Forests ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1077
Author(s):  
Agata Ćwik ◽  
Tomasz Wójcik ◽  
Maria Ziaja ◽  
Magdalena Wójcik ◽  
Katarzyna Kluska ◽  
...  
Keyword(s):  
Ecosystem Services ◽  
Positive Impact ◽  
Fungal Spores ◽  
Pollen Grains ◽  
Plant Composition ◽  
Artificial Lake ◽  
Negative Effect ◽  
Multi Media ◽  
The City ◽  
Main Attraction

Urban water bodies are an important asset in terms of climate change. The accompanying vegetation is an integral part of the waterside space system and a source of ecosystem services and disservices. The composition of greenery in waterside spaces should therefore be preceded by detailed research. This research would be the basis for the development of recommendations for enhancement of the positive impact of vegetation on humans and minimisation of its negative effect. The aim of the study was to identify ecosystem services and disservices of vegetation in the four most important waterside recreation spaces in the city of Rzeszów, Poland, and to develop plant composition guidelines. A detailed inventory of vegetation and aerobiological monitoring of the presence of airborne allergenic pollen grains and fungal spores were carried out. Next, the ecosystem services and disservices of the vegetation were determined based on literature data and on our expert judgement. Additionally, a counting of the number of visitors to waterside areas was conducted. All these steps were used to develop recommendations for shaping the vegetation of study areas. The results of the investigations show that the boulevards along the artificial lake function completely differently than other investigated places as the area resembles an urban park, and the water is not the main attraction in this space. The vegetation of the boulevards and the nearby gravel-pit bathing area has mostly a spontaneous character and offers the widest range of ecosystem services and disservices. The management of the vegetation should focus on its health-enhancing values. The vegetation growing near the outdoor swimming pools has been designed by man. Nevertheless, it requires recomposing and is targeted specifically at the enhancement of the visual attractiveness. Additionally, there is a need for planting compact deciduous trees that will provide shade at the multi-media fountain.

scholarly journals Airborne pollen observations using a multi-wavelength Raman polarization lidar in Finland: characterization of pure pollen types

2020 ◽  
Author(s):  
Xiaoxia Shang ◽  
Elina Giannakaki ◽  
Stephanie Bohlmann ◽  
Maria Filioglou ◽  
Annika Saarto ◽  
...  
Keyword(s):  
Optical Properties ◽  
Airborne Pollen ◽  
Pollen Grains ◽  
Wavelength Dependence ◽  
Depolarization Ratio ◽  
Mean Values ◽  
Pollen Types ◽  
Pine Pollen ◽  
Significant Wavelength ◽  
532 Nm

Abstract. We present a novel algorithm for characterizing the optical properties of pure pollen particles, based on the depolarization values obtained in lidar measurements. The algorithm was first tested and validated through a simulator, and then applied to the lidar observations during a four-month pollen campaign from May to August 2016 at the European Aerosol Research Lidar Network (EARLINET) station in Kuopio (62°44′ N, 27°33′ E), in Eastern Finland. Twenty types of pollen were observed and identified from concurrent measurements with Burkard sampler; Birch (Betula), pine (Pinus), spruce (Picea) and nettle (Urtica) pollen were most abundant, contributing more than 90 % of total pollen load, regarding number concentrations. Mean values of lidar-derived optical properties in the pollen layer were retrieved for four intense pollination periods (IPPs). Lidar ratios at both 355 and 532 nm ranged from 55 to 70 sr for all pollen types, without significant wavelength-dependence. Enhanced depolarization ratio was found when there were pollen grains in the atmosphere, and even higher depolarization ratio (with mean values of 25 % or 14 %) was observed with presence of the more non-spherical spruce or pine pollen. The depolarization ratio at 532 nm of pure pollen particles was assessed, resulting to 24 ± 3 % and 36 ± 5 % for birch and pine pollen, respectively. Pollen optical properties at 1064 nm and 355 nm were also estimated. The backscatter-related Ångström exponent between 532 and 1064 nm was assessed as ~ 0.8 (~ 0.5) for pure birch (pine) pollen, thus the longer wavelength would be better choice to trace pollen in the air. The pollen depolarization ratio at 355 nm of 17 % and 30 % were found for birch and pine pollen, respectively. The depolarization values show a wavelength dependence for pollen. This can be the key parameter for pollen detection and characterization.

scholarly journals Морфологические особенности пыльцы «ведьминых мётел» мутационного типа у сосны обыкновенной (Pinus sylvestris L.)

2017 ◽  
Vol 7 (4) ◽  
pp. 234-239
Author(s):  
M. S. Yamburov ◽  
S. B. Romanova ◽  
A. S. Prokopyev
Keyword(s):  
Pinus Sylvestris ◽  
Comparative Study ◽  
Scots Pine ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Abnormal Development ◽  
Tree Crown ◽  
Normal Part ◽  
Study Results ◽  
The Comparative Study

The comparative study results of pollen morphology of the mutational witches’ brooms and the normal part of the tree crown in Scots pine are presented. There is a decrease of pollen grains size, especially the sacci, in witches’ brooms. The witches’ brooms with more intensive branching have more expressive changes. Also, the witches’ brooms have more abnormal pollen grains. The data on the occurrence about 10 anomalous morphotypes of pollen grains are reported, most of that are related to the abnormal development of succi: different size of sacci, deformed sacci, reduced sacci, fused sacci, additional sacci, compress sacci, lack of one or both sacci. A high percentage of anomalies in the sacci development may be associated with less developed reticular sculpture of ectexine in witches’ brooms pollen.

scholarly journals Influence of High Frequency Electromagnetic Fields Produced by Antennas for Mobile Communication on the Structure of the Pancreas in Rats: Histological and Unbiased Stereological Analysis

2018 ◽  
Vol 68 (4) ◽  
pp. 484-501
Author(s):  
D.Smiljana Paraš ◽  
B. Radoslav Gajanin ◽  
Lj. Maja Manojlović ◽  
Nj. Zoran Ružić
Keyword(s):  
Electromagnetic Fields ◽  
Mobile Communication ◽  
Mobile Communications ◽  
High Frequency ◽  
Volume Density ◽  
Pancreatic Tissue ◽  
Male Rats ◽  
Numerical Density ◽  
Unexposed Group ◽  
Study Results

Abstract The emission of high frequency electromagnetic fields (HF EMF) produced by antennas for mobile communications has been controversially alleged to have adverse health effects. The aim of our work was to examine whether there are effects on living organisms from HF EMF produced by mobile communication antennas. In this experiment Wistar strain rats were exposed to HF EMF with the following characteristics: 1.9 GHz frequency, 0.24 A/m intensity, electric field strength of 4.79 V/m, and SAR (specific absorption rate) value of 2.0 W/m2. Exposure time was 7 hours per day, 5 days per week, over the course of sixty days. This experiment was conducted on a total of 30 male rats divided randomly into two equal groups: one group of animals was exposed to GSM fields (Global System of antennas for Mobile Communications) as described above whereas the other group of animals was not exposed to any GSM fields. In our study, results show that the quantity, diameter and numerical density of the islets of Langerhans in the pancreatic tissue increased in rats exposed to HF EMF compared to the unexposed group. The volume density, number and numerical density of pancreatic cells also changed in rats that were exposed to the HF EMF compared to the unexposed group. Our study shows a change in the stereological and histological parameters of rat pancreatic tissue due to the effects of HF EM fields produced by antennas for mobile communication.

PECULIARITIES OF THE RADIATION PATTERN OF THE BRAIN AND INTERNAL ORGANS OF NEWBORNS WHO UNDERWENT SEVERE PERINATAL ASPHYXIA AND UNDERWENT GENERAL THERAPEUTIC HYPOTHERMIA, ACCORDING TO THE PILOT STUDY RESULTS

2021 ◽  
Vol 100 (1) ◽  
pp. 30-35
Author(s):  
N.S. Vorotyntseva ◽  
◽  
V.V. Orlova ◽  
A.N. Motina ◽  
A.D. Novikova ◽  
...  
Keyword(s):  
Therapeutic Hypothermia ◽  
Perinatal Asphyxia ◽  
Control Group ◽  
Internal Organs ◽  
X Ray ◽  
Abdominal Organs ◽  
Study Results ◽  
Negative Effect ◽  
Chest X Ray ◽  
The Brain

Objective of the research: to study the features of the radiological picture of the brain and internal organs of newborns with severe perinatal asphyxia, who underwent general therapeutic hypothermia (GTH). Materials and methods: the study included 116 newborns with severe perinatal asphyxia. GTH was performed in 72 patients (group 1), 44 children did not receive hypothermia in the 2 (control) group. In the first 6 hours of life, children of groups 1 and 2 underwent ultrasound of the brain and abdominal organs and chest x-ray. The complex ultrasound scan was repeated at 3–5, 7–10, 14–16 and 21–28 days. Repeated chest x-ray was carried out strictly according to the indications. Results: GTH reduced the incidence of organic brain lesions by 18% in children with severe perinatal asphyxia (p0,05). The study revealed a number of undesirable consequences associated with GTH. Transient effusion into the abdominal and thoracic cavities was diagnosed by ultrasound in 8 (11%) patients after hypothermia, while there were no such changes in children in the control group (p<0,05). X-ray of the chest in newborns who received GTH during the first 14 days revealed edematous-hemorrhagic changes in the lungs more often than in patients of the control group – 55 (76%) and 24 (55%), respectively (p<0,05). Conclusions: GTH effectively prevented the development of severe post-hypoxic changes in the brain. The negative effect of hypothermia on microcirculation was manifested by the development of effusion into the serous cavities and edematous-hemorrhagic syndrome.

A CYTOLOGICAL AND MORPHOLOGICAL STUDY OF TWIN SEEDLINGS IN BROMUS INERMIS

1963 ◽  
Vol 43 (1) ◽  
pp. 18-22 ◽  
Author(s):  
A. C. Wilton ◽  
R. C. McGinnis ◽  
J. D. Truscott
Keyword(s):  
High Frequency ◽  
Morphological Study ◽  
Pollen Grains ◽  
Bromus Inermis ◽  
Germinated Seeds

A study was conducted on Bromus inermis Leyss. in an attempt to find haploids from twin seedling's. In 80,492 germinated seeds examined from 10 varieties, 298 twin seedlings were obtained, none of which was haploid. One triplo-diplo pair of twins was found, all others being diploid. The triplo-diplo twins had 83 and 57 chromosomes, respectively. A possible explanation of this phenomenon is presented. A high frequency of the diplo-diplo twins differed morphologically from one another. It is suggested that simultaneous fertilization by two pollen grains occurred in these cases. It was concluded that screening twin-seedlings cannot be recommended as a method of obtaining haploids in B. inermis.

scholarly journals Effects of Food Processing on In Vivo Antioxidant and Hepatoprotective Properties of Green Tea Extracts

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 572 ◽  
Author(s):  
Xiao-Yu Xu ◽  
Jie Zheng ◽  
Jin-Ming Meng ◽  
Ren-You Gan ◽  
Qian-Qian Mao ◽  
...  
Keyword(s):  
Green Tea ◽  
Food Processing ◽  
Antioxidant Activities ◽  
Ultrasound Treatment ◽  
Positive Effects ◽  
In Vivo Experiments ◽  
Study Results ◽  
Negative Effect

Food processing can affect the nutrition and safety of foods. A previous study showed that tannase and ultrasound treatment could significantly increase the antioxidant activities of green tea extracts according to in vitro evaluation methods. Since the results from in vitro and in vivo experiments may be inconsistent, the in vivo antioxidant activities of the extracts were studied using a mouse model of alcohol-induced acute liver injury in this study. Results showed that all the extracts decreased the levels of aspartate transaminase and alanine aminotransferase in serum, reduced the levels of malondialdehyde and triacylglycerol in the liver, and increased the levels of catalase and glutathione in the liver, which can alleviate hepatic oxidative injury. In addition, the differences between treated and original extracts were not significant in vivo. In some cases, the food processing can have a negative effect on in vivo antioxidant activities. That is, although tannase and ultrasound treatment can significantly increase the antioxidant activities of green tea extracts in vitro, it cannot improve the in vivo antioxidant activities, which indicates that some food processing might not always have positive effects on products for human benefits.

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