scholarly journals Effect of the solvent on the extraction of polyphenols from distillery stillage and on their antioxidant activity

2021 ◽  
Vol 17 ◽  
pp. 54-62
Author(s):  
Wioleta Mikucka ◽  
Magdalena Zielińska
Keyword(s):  
Antioxidant Activity ◽  
Raw Materials ◽  
Statistical Measure ◽  
Dpph Radical ◽  
New Directions ◽  
Polyphenolic Content ◽  
Radical Method ◽  
Food Market ◽  
High Antioxidant Activity ◽  
By Products

The increase in the costs of storage and disposal of post-production residues has resulted in the search for new directions for their recycling, which is closely related to the necessity of protecting the natural environment and promoting a circular economy. Moreover, the apparent interest shown by the food market in raw materials with high antioxidant activity implies an increasing use of by-products. The objective of the study was to determine the effect of the type and concentration of the solvent on the efficiency of extracting polyphenols from distillery stillage as well as their antioxidant activity by using several solvents: methanol:water (70:30 v/v), methanol:water (100:0 v/v), ethanol:water (70:30 v/v) or ethanol:water (100:0 v/v). The DPPH radical method was used to determine the antioxidant activity of the obtained extracts. The normalised variable (NV) and statistical measure (MS) were determined, based on which the effectiveness of the solvents was evaluated. The highest polyphenolic content and the antioxidant activity were obtained by using ethanol:water (70:30 v/v) as a solvent in the extraction of polyphenolic compounds from distillery stillage.

OBTAINING FRUIT AND BERRY EXTRACTS WITH HIGH ANTIOXIDANT ACTIVITY WHEN USING ULTRASONIC RADIATION

2018 ◽  
Author(s):  
Н.Б. ЕРЕМЕЕВА ◽  
Н.В. МАКАРОВА
Keyword(s):  
Antioxidant Activity ◽  
Raw Materials ◽  
Matter Content ◽  
Dry Matter Content ◽  
Black Currant ◽  
Ultrasonic Radiation ◽  
Microbiological Parameters ◽  
High Antioxidant Activity ◽  
Berry Extracts ◽  
Physical And Chemical

Подобраны оптимальные параметры для экстракции плодов и ягод черной смородины, малины, вишни, черноплодной рябины при ультразвуковом излучении 35 кГц с целью получения плодово-ягодных экстрактов с высокой антиоксидантной активностью. На основании экспериментальных данных выбраны технологические режимы процесса: экстрагирование сырья 75%-м этиловым спиртом при соотношении сырье : растворитель 1 : 10, температуре (40 ± 5)°C в течение 90 мин. Экстракты концентрируют под вакуумом до содержания сухих веществ 65%. Дана технологическая схема получения плодово-ягодных экстрактов. Полученные экстракты обладают высокой антиоксидантной активностью, содержат большое количество фенольных веществ, флавоноидов и антоцианов; по органолептическим, физико-химическим и микробиологическим показателям соответствуют нормативной документации. The optimal parameters for extraction of fruits and berries of fruits and berries of black currant, raspberry, cherry, chokeberry with ultrasonic radiation of 35 kHz in order to obtain fruit and berry extracts with high antioxidant activity are selected. Based on the experimental data, the technological modes of the process are selected: extraction of raw materials with 75% ethyl alcohol at a ratio of raw materials: solvent 1: 10, temperature (40 ± 5)°C for 90 min. The extracts are concentrated under vacuum to a dry matter content of 65%. Technological scheme of fruit and berry extracts is given. The obtained extracts have a high antioxidant activity, contain a large amount of phenolic substances, flavonoids and anthocyanins; organoleptic, physical and chemical and microbiological parameters correspond to the normative documentation.

scholarly journals Actividad antioxidante de extractos de hojas de Bocconia frutescens L. (Papaveraceae)

2016 ◽  
Vol 14 (2) ◽  
Author(s):  
Oscar Eduardo Rodríguez Aguirre ◽  
William Alejandro Andrade Barreiro ◽  
William Alejandro Andrade Barreiro ◽  
Fabio Eduardo Diaz Lopez ◽  
Fabio Eduardo Diaz Lopez
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Dpph Radical ◽  
Small Tree ◽  
High Antioxidant Activity ◽  
Para Determinar ◽  
Percent Uptake ◽  
Orange Color ◽  
Higher Sensitivity

<p>B<em>occonia frutescens </em>es un árbol pequeño, posee hojas profundamente lobuladas y con borde dentado, las flores están agrupadas en panículas terminales pendulares, las semillas son de color negro brillante con una cubierta carnosa de color anaranjada. Es nativa de América, es conocida con los nombres vulgares de: Sarno, Palo Amarillo o Trompeto. <strong>Objetivos: </strong>Evaluar la actividad antioxidante de extractos y fracciones de hojas de Bocconia frutescens por los métodos DPPH• y ABTS•+. <strong>Métodos: </strong>las fracciones obtenidas por soxleth con solventes de diferente polaridad de hojas, fueron evaluadas a concentraciones de 25, 62.5, 125 y 250 mg/LMeOH para determinar la actividad antioxidante por los métodos DPPH• y ABTS•+. <strong>Resultados: </strong>Para los extractos por el método decoloración del radical DPPH• el Porcentaje de Captación se encontró entre 47.6 y 57.7 a 250 mg/LMeOH y para las fracciones entre 45.4 y 54.1 mg/LMeOH, a 250 mg/LMeOH. Por el método decoloración del radical ABTS•+. Para los extractos el porcentaje de Captación se encontro entre 89.7 y 99.7 a 250 mg/LMeOH y para las fracciones entre 68.5 y 99.6 mg/LMeOH. <strong>Conclusiones: </strong>las fracciones de acetato de etilo y metanol presentaron una alta actividad antioxidante al ser evaluadas por las técnicas ABTS•+ y DPPH• , la técnica ABTS•+ se presento mayor sensibilidad.</p><p>Abstract</p><p>Bocconia frutescens is a small tree, has deeply lobed and serrated edge leaves, flowers are grouped in pendulous panicles terminal, seeds are shiny black with a fleshy covering of orange color. It is native to America, is known under their common names: Sarno, Palo Amarillo or Trompeto. Objectives: Evaluate the antioxidant activity of extracts and fractions of leaves Bocconia frutescens by DPPH• and ABTS•+ methods. Methods: the fractions obtained by Soxhlet with solvents of different polarity of leaves, were evaluated at concentrations of 25, 62.5, 125 and 250 mg / LMeOH to determine the antioxidant activity by DPPH• and ABTS•+ methods. Results: Extracts for the bleaching method DPPH• radical the Percent Uptake was between 47.6 and 57.7 to 250 mg / LMeOH and fractions between 45.4 and 54.1 mg / LMeOH, to 250 mg / LMeOH. By the method of coloration radical ABTS•+. Extracts for the percentage uptake was found between 89.7 and 99.7 and fractions between 68.5 and 99.6 mg / LMeOH, to 250 mg / LMeOH. Conclusions: The fractions of ethyl acetate and methanol have a high antioxidant activity to be evaluated by the ABTS•+ and DPPH• techniques, the technique ABTS•+ present higher sensitivity.</p>

scholarly journals Chemical Evaluation of a Nori-Like Product (Geluring) Made from the Mixture of Gelidium Sp. and Ulva Lactuca Seaweeds

2018 ◽  
Vol 6 (3) ◽  
pp. 664-671 ◽  
Author(s):  
ERNIATI ERNIATI ◽  
FRANSISKA RUNGKAT ZAKARIA ◽  
ENDANG PRANGDIMURTI ◽  
DEDE ROBIATUL ADAWIYAH ◽  
BAMBANG PONTJO PRIOSOERYANTO ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Dietary Fiber ◽  
Proximate Composition ◽  
Raw Materials ◽  
Food Product ◽  
Ulva Lactuca ◽  
Total Phenolic ◽  
Heat Application ◽  
High Antioxidant Activity

Dry sheet food products or nori-like products can be produced from the mixture of Gelidium sp. and Ulva lactuca seaweeds, which is called geluring. Making geluring involves heat application that may influence the chemical composition of the product. The goal of this study was to evaluate the chemical characteristics of geluring by measuring proximate composition; dietary fiber, total phenolic, and flavonoid contents; and antioxidant activity(by DPPH analysis)of geluring and compare the values to those of the raw materials. Three types of geluring were prepared following commercial nori preparation procedures with some modifications: P1 (unseasoned), P2 (seasoned), and P3 (seasoned and roasted).The proximate composition of geluring products and raw materials differed significantly(P<0.05). Geluring fiber contents were not significantly different (P>0.05) among P1 (29.19±0.26%), P2 (29.42±0.66%), and P3 (29.83±0.11%), but these values differed significantly (P<0.05) from those of the raw materials. The total phenolic content, flavonoid content, and DPPH activity of P2geluringwere 1.38 mg GAE/g, 1.11 mg QE/g, and 61.23%, respectively, which were significantly (P<0.05) higher than those of P1 and P3 but lower than those of the raw materials. These results suggest that geluring processing might negatively impact the chemical composition of the products, but they still have high antioxidant activity and dietary fiber content and thus have potential for utilization as a functional food product.

scholarly journals Effect of pepsin hydrolysis on antioxidant activity of jellyfish protein hydrolysate

2021 ◽  
Vol 302 ◽  
pp. 02010
Author(s):  
Pratchaya Muangrod ◽  
Wiriya Charoenchokpanich ◽  
Vilai Rungsardthong ◽  
Savitri Vatanyoopaisarn ◽  
Benjamaporn Wonganu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protein Hydrolysate ◽  
Inhibitory Effect ◽  
Raw Material ◽  
Degree Of Hydrolysis ◽  
Dpph Radical ◽  
Radical Scavengers ◽  
Hydrolysis Time ◽  
Hydrolysis Of ◽  
By Products

Edible jellyfish have been consumed as food for more than a century with offering high protein and crunchy texture. The pepsin hydrolysis of jellyfish protein yields jellyfish protein hydrolysate (ep-JPH), reported for potential bioactivities such as antioxidant activity or antihypertensive activities. Due to the substantial number of by-products generated from jellyfish processing, the by-products were then selected as a raw material of JPH production. This research aimed to evaluate the effect of the hydrolysis time of pepsin on the antioxidant activity of ep-JPH. The dried desalted jellyfish by-products powder was enzymatically hydrolysed by 5% (w/w) pepsin, and the hydrolysis time was varied from 6, 12, 18, and 24 h at 37oC. Results showed that increased hydrolysis time increased the degree of hydrolysis (DH) and inhibition of DPPH radical. The 24 h ep-JPH possessed the highest DH and the highest inhibitory effect of DPPH radical. The results demonstrated that, in this experiment, all ep-JPHs were DPPH radical scavengers, exhibiting different inhibition activities depending on DH values.

Relative Antioxidant Activity of Common Indian Herbs

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Padma S Vankar ◽  
Jyoti Srivastava
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Ash Content ◽  
Total Phenol ◽  
Dpph Radical ◽  
Medicinal Purpose ◽  
Frap Assay ◽  
Plant Materials ◽  
High Antioxidant Activity ◽  
Very High

Ten Indian herbs (Manjisth, Tagar, Kulfa, Khuskhus, Gokharu, Carrot, Kasni, Bathua, Soya ke Beej, Pudina), popularly used for medicinal purpose, were extracted in sonicator by methanol and water for the estimation of polyphenols and flavonoids. Antioxidant capacities (AOC) of extracts were evaluated based on their ability to scavenge the DPPH radical and FRAP Assay. The results showed that methanolic extract of all the plant materials had very high antioxidant activity. Amongst them, Pudina extract in the methanol showed highest value of AOC by both FRAP and DPPH methods (33 percent and 1.36 mg QEq / 100 g). Total phenol and flavonoids content were also evaluated for these extracts. It varied from 0.8736 to 6.3918 mg GAE / 100 g and 0.3642 to 1.9876 mg QE / 100 g. Total ash content and acid insoluble content of soya showed highest value i.e. 14.55 percent and 92.92 percent.

scholarly journals EVALUATION AND OPTIMIZATION OF ANTIOXIDANT POTENTIALITY OF XYLARIA FEEJEENSIS HMJAU22039

2016 ◽  
pp. 269 ◽  
Author(s):  
Durga Pallavi Rebbapragada ◽  
Rajagopal Kalyanaraman
Keyword(s):  
Antioxidant Activity ◽  
Yeast Extract ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Basal Medium ◽  
Tectona Grandis ◽  
Total Phenolic ◽  
Dpph Radical ◽  
High Antioxidant Activity ◽  
Dpph Radical Scavenging

<p>ABSTRACT<br />Objective: Antioxidants neutralize free radicals generated in the human body and prevent them from causing damage. The present study is aimed to<br />evaluate and optimize the antioxidant activity of Xylaria feejeensis HMJAU22039 an endophyte isolated from Tectona grandis.<br />Methods: The antioxidant potential was measured by 1-1diphenyl-2-picryl-hydrazil (DPPH) radical scavenging assay and nitric oxide (NO) scavenging<br />assay. Total phenolic content (TPC) of the samples was measured by Folin–Ciocalteu reagent based assay, and the values were obtained from the<br />regression equation: y = 0.006141x + 0.157733.<br />Results: Potato dextrose yeast extract broth medium was selected as the basal medium as it reported a high antioxidant activity. The basal medium<br />was optimized with 2% dextrose, 0.2% yeast extract, and 200 g/L potato infusion. Dextrose and yeast extract were selected as carbon and nitrogen<br />sources, respectively, as they reported high antioxidant activity. The antioxidant activity and phenolic content are highest at static condition<br />(18.14 mg/g). Incubation temperature of 30°C and pH of the initial medium at 6 were found to be optimum conditions for high antioxidant activity. An<br />incubation period of 20 days reported the highest antioxidant activity and phenolic content. Methanol extract recorded high antioxidant activity with<br />a DPPH (73.86%) followed by ethyl acetate and chloroform fractions.<br />Conclusion: The study highlights the importance of different physiochemical parameters in the production of secondary metabolites having<br />antioxidant properties. The results reveal a significant positive correlation between DPPH radical scavenging assay, NO scavenging assay, and TPC.<br />Keywords: 1-1diphenyl-2-picryl-hydrazil, Total phenolic content, Endophytic fungus, Tectona grandis.</p>

scholarly journals The Bioactive Compounds and Antioxidant Activity of Food Products of Rhizophora stylosa Fruit (Coffee and Tea Mangrove)

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Dewi Indah Miranti ◽  
Hideaki Ichiura ◽  
Yoshito Ohtani
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Raw Materials ◽  
Precipitation Method ◽  
Dodecanoic Acid ◽  
Methanol Fraction ◽  
Rhizophora Stylosa ◽  
H Nmr ◽  
High Antioxidant Activity ◽  
C Nmr

The objective of this study is to investigate the bioactive compounds and antioxidant activity of coffee and tea mangrove (locally known in Indonesia) produced from the fruit of Rhizophora stylosa. Furthermore, three raw materials of coffee mangrove were also investigated to clarify their potencies. The crude extracts of five samples were subjected to antioxidant assay using DPPH. The results show that the extract of tea mangrove has the strongest activity; then, it was successfully fractionated using different polarity of solvents and yielded acetone and methanol fractions that had high antioxidant activity. The acetone fraction was purified and gave fractions A1, A2, A3, A4, A5, and A6, but only A2 and A3 indicated antioxidant activity and, therefore, they were subjected to further purification. Fractions A3 (caffeine) and A2 gave AS1 (N,N-dimethyl-L-alanine), AS2 (quercetin-3-O-galactopyranoside), AS3 (dodecanoic acid), and AS4 that had the similar 1H-NMR spectrometric results with AS2, while the methanol fraction did not exhibit clear peaks on the chromatogram by HPLC. Therefore, the precipitation method was conducted to purify this fraction, and the precipitate was analyzed by NMR spectra. The results from 1H and 13C NMR indicate that this fraction is a typical polymer of condensed tannins, containing procyanidin and prodelphinidin units.

scholarly journals SYNTHESIS AND PRELIMINARY EVALUATION OF 3,3’-DIHYDROXY-4,4’-DIMETHOXYDIBENZYLIDENEACETONE AS SUNSCREEN AND ANTIOXIDANT ACTIVE COMPOUND

2018 ◽  
Vol 16 (1) ◽  
pp. 48
Author(s):  
Harizal Harizal ◽  
Ariyo Prabowo Hidayanto ◽  
Nindya Wulan Sari
Keyword(s):  
Antioxidant Activity ◽  
Active Compound ◽  
Condensation Reaction ◽  
Radical Scavenger ◽  
Preliminary Evaluation ◽  
Dpph Radical ◽  
Activity Test ◽  
High Antioxidant Activity ◽  
Acid Catalyzed ◽  
Absorbance Profile

Synthesis and preliminary evaluation of 3,3’-dihydroxy-4,4’-dimethoxydibenzylidenacetone (DDB) as sunscreen and antioxidant have been successfully conducted. The compound was prepared throught acid catalyzed condensation reaction between vanillin and aceton using saturated HCl solution in glacial acetic acid. Pre-evaluation of sunscreen active compound candidate was performed by determining electronic absorbance profile, lamdamax, e, lamda c, UVA/UVB ratio, and photostability. While antioxidant activity test was performed using DPPH radical scavenger method. Based on the results obtained, the compound obtained has very good protective effect in UVA regions with high antioxidant activity.

scholarly journals Sugar content, acidity and antioxidant activity in dehydrated blackcurrant

2020 ◽  
Vol 82 (1) ◽  
pp. 183-186
Author(s):  
D. I. Barbashin
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Shelf Life ◽  
Raw Materials ◽  
Osmotic Dehydration ◽  
Sugar Content ◽  
High Water ◽  
Raw Material ◽  
High Water Content ◽  
High Antioxidant Activity

The main reason for spoilage of berry raw materials is the high water content in it. And in order to increase the shelf life of such raw materials and products based on it, various dehydration methods are used. Osmotic dehydration, considered in this article, is one of the best and suitable methods for increasing the shelf life of berry products, as well as increasing the biological value of the product. This method is preferred relative to other methods of dehydration due to the fact that when it is used in raw materials, more vitamins and minerals are preserved, and the color, aroma and taste of berries are also better preserved. Osmotic dehydration is a process due to the presence of semi-permeable membranes, during which the concentration is balanced. Osmosis takes place during the immersion of fruits in concentrated solutions of osmotically active substances. In such a system, two opposite processes occur: water diffuses from the product into the solution, and the dissolved substance diffuses from the solution into the product. This article discusses the features of the process of osmotic dehydration of blackcurrant berries. Blackcurrant is a promising raw material for processing enterprises. The berry contains a large amount of ascorbic acid, anthocyanins and has a high antioxidant activity. Methods were studied for samples of dried blackcurrant berries (by convection), with preliminary osmotic dehydration with various osmotic agents (sucrose, flower honey), using the following methods: ascorbic acid and antioxidant activity were determined titrimetrically using the Folin-Ciocalteu reagent polyphenols, spectrophotometrically anthocyanins.

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