Efficacy of bioagents and botanicals on seed mycoflora and seed quality in mungbean [Vigna radiata (L.) Wiczek]

Author(s):  
S. B. Gawade ◽  
S. R. Zanjare ◽  
A. V. Suryawanshi ◽  
V. R. Shelar

Seeds of mungbean (Cv. Vaibhav) was treated with bioagents and botanicals <italic>viz;</italic> talc powder formulations of <italic>Pseudomonas fluorescens</italic> (0.6%), <italic>Trichoderma viride</italic> (0.6%), <italic>Pseudomonas fluorescens + Trichoderma viride</italic> (0.6%) each, garlic extract (1%), ginger extract (1%) and Thiram + Carbendazim (0.2% each) as a recommended fungicidal check. The results indicated that, among the bioagents the treatment of <italic>Pseudomonas fluorescens + Trichoderma viride</italic> (0.6% each) to the seeds of mungbean was effective in reducing seed mycoflora <italic>viz; Fusarium oxysporum, Alternaria alternata, Macrophomina phaseolena</italic>, <italic>Aspergillus flavus, Aspergillus niger</italic> and <italic>Fusarium moniliforme</italic> by 88.5, 85.1, 64.9, 81.1, 75.0 and 100 per cent, respectively over untreated control. The seed germination, seedling vigour index and field emergence of mungbean was increased by 21.81, 34.03 and 15.98 per cent, respectively with this treatment over untreated control. The seed germination, seedling vigour index and field emergence in untreated control was 71.83 per cent, 2261.52 and 67.83 per cent, respectively.

Author(s):  
M. Ananthi ◽  
P. Selvaraju ◽  
K. Sundaralingam

Laboratory experiment was carried out using ‘PKM 1’ chilli (Capsicum annuum L.) seed to standardize bioprimed seeds under moisture stress condition inorder to improve seed germination and seedling vigour. To induce the moisture stress, optimize the different concentration of water holdings capacity viz., 80, 60, 40 and 20% were used for best bioprimed seed treatment (biocontrol agents (Pseudomonas fluorescens 60% 12 h, Trichoderma viride 60% 9 h) and liquid biofertilizers (Azospirillum 10% 9 h and Phosphobacteria 15% 9h )) along with hydroprimed seed and control seed. Seed bioprimed with Pseudomonas fluorescens 60% for 12 h improved the germination percentage ( 82), root length (14.2 cm), shoot length (6.2 cm), dry matter production (0.0489 g 10 seedlings-1) and vigour index (1673) compared to control seed. Seed biopriming with  Pseudomonas fluorescens 60% for 12 h can be adopted to improve seed germination and seedling vigour under moisture stress condition upto 20%.


2017 ◽  
Vol 9 (2) ◽  
pp. 1226-1235
Author(s):  
B.N. Raghu ◽  
Basave Gowda ◽  
S. N. Vasudevan ◽  
Sangeeta I. Macha ◽  
Sharan Gouda Hiregoudar ◽  
...  

A laboratory experiment was conducted to know the effect seed treatment with nano insecticides on seed quality of pigeonpea (Cajanus cajan (L.) Millsp.) cv. TS3R. This study was conducted to evaluate the effect of macro and nano insecticides on seed germination and vigour of Pigeonpea. Different recommended seed treatment insecticides viz, malathion, fenvalerate, emamectine benzoate, thiodicarb, sweet flag and neem seed kernel powder insecticides were synthesized to nano form using high energy planetary ball mill. The Pigeonpea seed were treated with different nano insecticides i.e., 10-90 per cent reduction in actual dosage. Among the different treatments studied, seed treated with nano malathion 50 per cent lesser than normal dosage, fenvalerate 60 per cent lesser, thiodicarb 10 per cent lesser, emamectine benzoate 30 per cent lesser, sweetflag 70 per cent lesser, neem seed kernel powder 40 per cent lesser than actual recommended dosage gave significantly higher seed germination (98.0, 98.67, 98.67, 97.0, 99.0 and 98.67 percent) ,less number of abnormal seedlings (1.0, 0.33, 1.0, 1.0, 1.0 and 0.33 per cent) , shoot length (10.13, 9.00, 11.47, 9.50, 10.90 and 10.87 cm), root length (12.56, 12.93, 12.83, 12.60 11.50 and 13.00 cm), seedling dry weight (85.73, 87.40, 88.47, 87.70, 88.60 and 88.27 g) and seedling vigour index (2223, 2164, 2397, 2143, 2217 and 2354) as compared to untreated seeds and macro insecticides. Therefore, it is very clear that nano based insecticides has a significant (0.1 %) impact on the seed quality improvement.


Author(s):  
P. Lakshmi Pravallika ◽  
S.L. Bhattiprolu ◽  
K. Radhika ◽  
M. Raghavendra

Background: Sesame is an important oil seed crop affected by toxigenic fungal pathogens viz., Alternaria, Fusarium and Aspergillus. Among these, Alternaria sesami is seed borne and most destructive pathogen causing yield loss to an extent of 28.9 per cent. Hence the effect of artificial infection with A. sesami on seed quality of various samples of sesame was investigated during 2017-2018. Methods: A total of 28 samples were collected from different sesame growing areas of Andhra Pradesh and Telangana and inoculated with A. sesami. The inoculated and uninoculated seeds were tested for germination and seedling growth by rolled paper towel method.Result: Significant differences in seed germination, seedling length and seedling vigour index were observed among the uninoculated as well as inoculated sesame seed samples. The results indicated that Alternaria sesami caused 15.13% to 49.68%, 12.77% to 46.14% and 28.28% to 72.87% reduction in seed germination, seedling length and vigour index, respectively, of inoculated seed over uninoculated seeds. The per cent reduction was highest in the seed samples of YLM-17 collected from farmers’ saved seed samples of Prakasam district.


Author(s):  
S. Kavitha ◽  
P. Srimathi

Background: Black gram is the important pulses crop, grown throughout the country; gives low seed yield mainly due to poor management and low soil fertility. Development of suitable technology is essential to enhance productivity. Seed priming is one such effective technology which enhance rapid and uniform emergence to achieve high vigour, leading to better stand establishment and yield. The current research aimed to study micronutrient seed priming and rhizobium coating on seed germination, seedling vigour, crop growth and seed yield in black gram.Methods: Blackgram seeds were primed with 10 different micronutrient solutions with different concentrations and evaluated for its quality parameters to find out suitable seed priming technique. Seeds from two best priming treatments were coated with rhizobium @ 30g/kg of seed and evaluated for its production potential and resultant seed quality under field condition.Result: Seed priming with 0.5% ZnSO4 at 1/3rd seed to solution ratio for 3 hours recorded higher germination (96%) and vigour index (3811) than control which recorded 84% seed germination and 2528 of vigour index under laboratory experiment. Field experiments revealed that, seeds primed with 0.5% ZnSO4 and coated with rhizobium @ 30g per kg of seeds improved seed yield by 13.2% over control. 


Author(s):  
Pramod Sharma ◽  
Arun Bhatt ◽  
Rajesh Kanwar

The present investigation was carried out in the Department of Seed Science and Technology, College of Forestry, Ranichauri, Tehri Garhwal, Uttarakhand during 2015. A laboratory experiment was conducted to find out the influence of bio-priming on seed germination and seedling vigour. Seed germination was reported maximum for Psf-173 (93.66%) while minimum was reported for control (65.66%). Seed vigour parameter viz. root length (11.01 cm), shoot length (7.96 cm), seedling length (18.98 cm), fresh weight (8.00 g) and dry weight (1.31 g), vigour index I (1666.80) and vigour index II (115.56) was found higher for PSB treatment. Shilajeet variety had recorded significant higher values for most of the germination and vigour parameters. Shilajeet bio-primed with PSB attained higher values for majority of the quality parameters. Under laboratory condition results suggested that seed germination and seed vigour were greatly affected by different bio-agents. Among all the bio-agents, PSB showed positive influence on most of the quality parameter. However, other bio-agents had also showed significant effect over control.


2020 ◽  
Vol 8 (4) ◽  
pp. 437-447
Author(s):  
R.A.I.S. Ariyarathna ◽  
S. L. Weerasena ◽  
C. K. Beneragama

Tomato is one of the most commonly growing vegetable crops among the farmers in Sri Lanka. Standard laboratory germination of tomato seeds fulfills the regulatory requirements of seed marketing. However, poor field performance is an overwhelming problem to farmers. Present study was focused on the longevity of two varieties of tomato seeds as affected by their quality characters (percentage germination, moisture, field emergence and vigour index, seed protein and carbohydrate contents) including seed health (exposure of Clavibacter michiganensis subsp. Michiganensis (CMMV), Spotted wilt virus (TSWV) and Leaf curly top virus (LCTV)) under four different storage environmental conditions during a one-year long storage study. Seeds were packed in triple-laminated aluminum packets (TLA) and stored in low temperature storage conditions (17±1 °C and 65% RH) and in ambient conditions in Gannoruwa, Kundasale, and MahaIlluppallama that represent the three agro-ecologically zones; Mid Country Wet Zone (MCWZ), Mid Country Intermediate Zone (MCIZ) and Low Country Dry Zone (LCDZ) respectively. Seed quality parameters displayed varied responses depending on the variety. Physiological changes that occur in seeds due to temperature, RH, changes in proteins and carbohydrate content, CMMV and TSWV during storage were manifested as reduction in seed germination, field emergence and vigour index. However, none of the seedling carried LCTV and therefore its effect on seed quality parameters could not be assessed. The seeds could be stored for ca. 29 months at low and constant temperatures and RH conditions without compromising local seed germination standards compared to ambient storage in the three environments. CMMV and TSWV detected in all seed samples indicated potential threats to tomato farmers in Sri Lanka.  


Author(s):  
N. Aiswarya ◽  
S.L. Bhattiprolu ◽  
K. Bayyapu Reddy ◽  
G. Rama Rao

Background: Aspergillus niger and A. flavus cause rotting, damping-off, loss in germination and toxification of oil seeds. Aspergillus is a common mold in tropical and Sub-tropical countries and causes aflatoxin contamination. A. flavus and A. niger were responsible for storage rot of groundnut. In addition to storage rot, aflatoxin contamination also leads to adverse effects on consumer health. Therefore, present study was conducted to estimate the impact of both A. niger and A. flavus on seed quality parameters of groundnut. Methods: Effect of major seed borne fungi A.niger and A. flavus on seed quality characters of thirteen different genotypes of groundnut was observed in the present study. All the seedling quality characters were measured as per ISTA guidelines. Result: On an average A. niger caused 25.14, 25.18, 43.99, 26.20 and 44.75 per cent reduction in seed germination, seedling length, seedling vigour index I, seedling dry weight and seedling vigour index II, respectively, over the control whereas A. flavus caused average per cent reduction of 22.38, 24.23, 41.18, 23.92 and 40.95 in seed germination, seedling length, seedling vigour index I, seedling dry weight and seedling vigour index II, respectively, over the control.


2015 ◽  
Vol 9 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Anişoara Stratu ◽  
Naela Costică

AbstractThe paper presents the results of a study regarding the influence of treatment with zinc in different concentrations (50 mg∕l, 100 mg∕l, 200 mg ∕l, 300mg/l, 400 mg ∕l, 500 mg/l, 600 mg ∕l) on seed germination and growth in early ontogenetic stages ofCucumis meloL. We analyzed the following indicators: the percentage of germinated seeds; the length of root, the length of the hypocotyl and the length of the seedling; the number of the laterale roots; the tolerance index and the seedling vigor index. The results underline the specific variations of analysed indicators, depending on the concentrations used for the treatments of seeds. The concentrations used for treatment do not influence negatively the seed germination, but affected the seedling growth (especially the root elongation), the formation and growth process of lateral roots and the seedling vigour index. The delay effect of growth process is very pronounced in the case of high concentration.


2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.


2017 ◽  
Vol 3 (1) ◽  
pp. 140-144
Author(s):  
A Rokib ◽  
MS Monjil

Effectiveness of six fungicides viz., Provax-200 (Carboxin+Thiram), Bavistin DF (Carbendazim), Dithane M-45 (Mancozeb), Secure (Mancozeb+Fenamidone), Antracol (propineb) and Daconil (Chlorothalonil) were evaluated to improve seed germination and seedling vigour of lentil variety BINA Masur-3. The experiment was conducted in the Green House of Seed Pathology Centre, Bangladesh Agricultural University, Mymensingh. Positive effect of seed treatment with fungicides on seed germination and seedling vigour of lentil was recorded. At 20 DAS, increased seed germination over control was observed in treated seeds with Deconil (10.40%) and Antracol (10.00%) followed by Dithane M-45 (7.20%). Seeds treated with Deconil and Dithane M-45 produced seedlings with higher shoot length, root length and seedling vigour. At 20 DAS, higher percent increased vigour index over control was found in Dithane M-45 (24.64%) and Deconil (22.44%), respectively.Asian J. Med. Biol. Res. March 2017, 3(1): 140-144


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