Effect of ESR, FSHB and PRLR Genes on Sperm Traits of Landrace and Yorkshire Boars in the Tropical Environmental Conditions of Vietnam

2020 ◽  
Author(s):  
Do Duc Luc ◽  
Ha Xuan Bo ◽  
Nguyen Hoang Thinh ◽  
Nguyen Chi Thanh ◽  
Tran Xuan Manh ◽  
...  
Keyword(s):  
Mixed Model ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Quality Trait ◽  
Quality Traits ◽  
Tissue Samples ◽  
Genotype Frequencies ◽  
Tropical Conditions ◽  
Sperm Traits ◽  
Esr Gene

Background: Fertility traits in pigs made a restricted progress through traditional selection. Applying marker assisted selection could improve these traits. The aim of the study was to investigate the effects of candidate genes Estrogen Receptor (ESR), Follicle Stimulating Hormone Beta (FSHB) and Prolactin Receptor (PRLR) on sperm quality traits of Landrace and Yorkshire boars under tropical conditions in Northern Vietnam.Methods: A total of 6306 ejaculates from 140 boars (92 Landrace and 48 Yorkshire) were collected to estimate sperm ejaculate volume (VOL), spermatozoon motility (MO), sperm concentration (CO) and total number of spermatozoon in ejaculate (NT). Ear tissue samples were collected for genotype identification of SNP present in ESR, FSHB and PRLR genes using PCR-RFLP technique. A mixed model was used to test the effect of different genetic and non-genetic factors on the sperm quality traits.Result: The genotype frequencies of ESR, FSHB and PRLR were in Hardy-Weinberg equilibrium for each breed. Age of boars and month of the year had significant effect on all the sperm quality traits (P less than 0.01) while effect of breed was found to be non-significant on all the sperm quality trait. FSHB gene significantly (P less than 0.05) affected VOL, MO and CO. Boars with BB genotype showed positive effect on VOL but negative effect on MO and CO in comparison with AA genotype boars. ESR gene showed only effect on VOL while PRLR affected only MO. Boars with AA and AB genotypes of ESR gene had a significantly higher VOL than those with BB genotype (P less than 0.05). For PRLR gen, AB genotype was associated with higher MO than AA genotype (P less than 0.05). These results suggest a possibility of using the existing polymorphisms in ESR, FSHB and PRLR genes to improve some sperm traits of Landrace and Yorkshire boars.

scholarly journals Mobile phones affect multiple sperm quality traits: a meta-analysis

F1000Research ◽  
2013 ◽  
Vol 2 ◽  
pp. 40 ◽  
Author(s):  
Madhukar Shivajirao Dama ◽  
M Narayana Bhat
Keyword(s):  
Mobile Phone ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Meta Analysis ◽  
Sperm Concentration ◽  
Reproductive Age ◽  
Quality Traits ◽  
Motile Sperm ◽  
Mobile Phone Use ◽  
Mobile Phone Radiation

As mobile phone usage is growing rapidly, there is a need for a comprehensive analysis of the literature to inform scientific debates about the adverse effects of mobile phone radiation on sperm quality traits. Therefore, we conducted a meta-analysis of the eligible published research studies on human males of reproductive age. Eleven studies were eligible for this analysis. Based on the meta-analysis, mobile phone use was significantly associated with deterioration in semen quality (Hedges’s g = -0.547; 95% CI: -0.713, -0.382; p < 0.001). The traits particularly affected adversely were sperm concentration, sperm morphology, sperm motility, proportion of non-progressive motile sperm (%), proportion of slow progressive motile sperm (%), and sperm viability. Direct exposure of spermatozoa to mobile phone radiation with in vitro study designs also significantly deteriorated the sperm quality (Hedges’s g = -2.233; 95% CI: -2.758, -1.708; p < 0.001), by reducing straight line velocity, fast progressive motility, Hypo-osmotic swelling (HOS) test score, major axis (µm), minor axis (µm), total sperm motility, perimeter (µm), area (µm2), average path velocity, curvilinear velocity, motile spermatozoa, and  acrosome reacted spermatozoa (%). The strength of evidence for the different outcomes varied from very low to very high. The analysis shows that mobile phone use is possibly associated with a number of deleterious effects on the spermatozoa.

scholarly journals Fertility and Sperm Quality of 1- and 2-year-Old Native Ganders Used for Natural Mating and Artificial Insemination

2021 ◽  
Author(s):  
Mehmet Akif BOZ ◽  
Hatice BAŞ ◽  
Musa SARICA ◽  
Kadir ERENSOY
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Egg Production ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Day Length ◽  
Quality Traits ◽  
Female Ratio ◽  
Semen Volume ◽  
Natural Mating

Abstract In the present study, the fertility rate and egg-hatching results from either natural mating or artificial insemination using 1- and 2-year-old domestic Turkish geese were compared. Sperm quality traits of 1- and 2-year-old ganders used for mating were determined. The study comprised 72 two-year-old females, 12 one-year-old, and 12 two-year-old ganders at the beginning of the laying period. Thirty-six female geese were mated naturally (18 with 1-year-old ganders, 18 with 2-year-old ganders); the remaining 36 were artificially inseminated (18 with 1-year-old ganders, 18 with 2-year-old ganders). Twenty-four ganders were separated into groups (12 for natural mating; 12 for artificial insemination). The male-to-female ratio in both mating protocols was 1:3. The geese were housed during the laying period in a natural and artificially ventilated house under natural lighting (increased day length). Quality traits, such as semen volume, sperm concentration, sperm quality factor (SQF), sperm motility, and some sperm morphological properties were determined. Fertility was higher in the groups containing 2-year-old ganders than in those using 1-year-old ganders (P < 0.05). The effect of insemination on semen volume, sperm concentration, SQF, sperm motility, total live sperm, normal sperm, macrocephalus sperm, and dead sperm was determined to be significant (P < 0.05). As a result, there is a need to improve the egg production and broody behavior traits of domestic Turkish geese. In addition, the use of 2-year-old geese in artificial insemination is appropriate and will contribute positively to the breeding and selection process.

scholarly journals Characterisation of sperm piRNAs and their correlation with semen quality traits in swine

2020 ◽  
Author(s):  
M. Ablondi ◽  
M. Gòdia ◽  
J. E. Rodriguez-Gil ◽  
A. Sánchez ◽  
A. Clop
Keyword(s):  
Transposable Elements ◽  
Genome Analysis ◽  
Genome Stability ◽  
Semen Quality ◽  
Sperm Quality ◽  
Quality Trait ◽  
Quality Traits ◽  
Male Germline ◽  
Long Interspersed Nuclear Elements ◽  
Non Coding Rnas

SummaryPiwi-interacting RNAs (piRNAs) are a class of non-coding RNAs which main reported function consists on the silencing of transposable elements and genome stability in mammalian germline. In this study we have identified piRNAs in porcine sperm, using male germline and zygote datasets from human, mice, cow and swine, and evaluated the relation between their abundances and sperm quality traits. Our analysis identified 283,382 piRNAs, 1,355 of which correlated to at least one semen quality trait. Indeed, genome analysis of the correlated piRNAs evidenced that 57% of these were less than 50kb apart and were significantly enriched near Long Interspersed Nuclear Elements (LINEs). Moreover, some of the significant piRNAs mapped within or close to genes relevant for fertility or spermatogenesis such as CSNK1G2 and PSMF1.

scholarly journals Spermatogenic Activity and Sperm Traits in Post-Pubertal and Adult Tomcats (Felis catus): Implication of Intra-Male Variation in Sperm Size

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 624
Author(s):  
Eliana Pintus ◽  
Martin Kadlec ◽  
Barbora Karlasová ◽  
Marek Popelka ◽  
José Luis Ros-Santaella
Keyword(s):  
Cell Function ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Sperm Head ◽  
Mitochondrial Activity ◽  
Head Width ◽  
Dna Integrity ◽  
One Year ◽  
Sperm Size ◽  
Sperm Traits

Tomcats are considered to be adults at 1 year of age, although many reach sexual maturity at an earlier age. Nevertheless, we still know little about whether the spermatogenic activity and sperm quality of mature under one-year-old tomcats differ from those of tomcats that are over one-year-old. This study aims to evaluate the spermatogenic activity, sperm traits, and their relationships in mature tomcats at two different ages. Sixteen tomcats showing complete spermatogenesis and spermatozoa in their epididymal caudae were used and classified according to their age as post-pubertal (<1 year old) or adult (˃1 year old). Our results show that adult cats have higher epididymal sperm concentration and lower coefficient of variation in sperm head width and ellipticity than post-pubertal cats. However, they do not differ in their testicular and epididymal mass, spermatogenesis, and sperm traits such as motility, mitochondrial activity, morphology, morphometry, as well as plasma membrane, acrosome, and DNA integrity. Reduced intra-male variation of sperm head ellipticity is associated with higher testis mass, epididymis mass, and sperm concentration. Interestingly, low intra-male variation in sperm head size is associated with increased Sertoli cell function and reduced post-meiotic germ cell loss. These findings increase our knowledge about feline reproductive physiology and provide new insights into the functional significance of low intra-male variation in sperm size and shape in tomcats.

SNP Identification in Sperm Associated Antigen 11B Gene and Its Association with Sperm Quality Traits in Murrah Bulls

2021 ◽  
Author(s):  
B. Deshmukh ◽  
A. Verma ◽  
I.D. Gupta ◽  
N. Kashyap ◽  
J. Saikia
Keyword(s):  
Sperm Quality ◽  
Polymorphic Information Content ◽  
Large Population ◽  
Immune Recognition ◽  
Quality Traits ◽  
Seminal Parameters ◽  
Genotype Frequencies ◽  
The Impact ◽  
Novel Snp ◽  
Acrosomal Integrity

Background: The beta-defensin family antigens present on sperm surface suggestively play pivotal role in sperm fertility by aiding in various steps of sperm maturation, motility, capacitation, immune recognition and sperm-oocyte interaction in female tract. It is imperative to explore genetic polymorphisms to build a better understanding of individual variation in male fertility. The experiment was designed to identify DNA polymorphism in Sperm Associated Antigen 11B (SPAG11B) gene and to analyze association between genetic variants with sperm quality traits in buffalo bulls (Murrah) in ICAR-National Dairy Research Institute, Karnal. Methods: Genomic DNA was extracted from hundred and thirty Murrah bulls. A 395 base pair region covering partial Intron 2, Exon 3 and Partial Intron 3 of bovine SPAG11 gene was amplified and genotyped using PCR-RFLP method. The PCR amplified product was purified, sequenced and further ClustalW analysis was done to align edited sequence with reported Bos taurus sequence (AC_000158.1). Gene and genotype frequencies, effective number of alleles, level of heterozygosity and polymorphic information content of various genotypes were estimated by standard procedure. Seminal parameters (Post thaw sperm motility, sperm viability, acrosomal integrity, HOST and abnormality) were estimated and statistical analysis was carried out. Result: A novel SNP (G greater than A substitution) at 2266 base of the SPAG11B gene was identified by sequencing. The fragment of SPAG11 gene containing 395 base pairs was amplified using PCR and digested with restriction enzyme i.e. MunI which showed three distinct genotypes viz., AA (266 bp and 107 bp fragment), AG (307 bp, 266 bp and 107 bp) and GG (373 bp fragment). Least squares means of seminal parameters for the SNP was estimated and compared after correction for non-genetic factors. Between genotypes, significant differences were found only for acrosomal integrity and was highest in AG (74.22±0.72) followed by AA (72.6±0.89) and GG (71.12±0.97), respectively. The identified novel SNP of SPAG11 gene showed significant association with acrosome integrity with genotype AG being superior to other genotypes. However, an association cannot be established with other seminal parameters with this SNP, further studies are required in order to validate the impact of g.2266G greater than A on sperm quality traits in a large population.

scholarly journals Combining metabolomic and transcriptomic approaches to assess and improve crop quality traits

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Delphine M. Pott ◽  
Sara Durán-Soria ◽  
Sonia Osorio ◽  
José G. Vallarino
Keyword(s):  
Quality Trait ◽  
Quality Traits ◽  
Crop Species ◽  
Breeding Programs ◽  
Crop Quality ◽  
Crop Varieties ◽  
New Genes ◽  
Technical Advances ◽  
Health Promoting ◽  
Selection Of

AbstractPlant quality trait improvement has become a global necessity due to the world overpopulation. In particular, producing crop species with enhanced nutrients and health-promoting compounds is one of the main aims of current breeding programs. However, breeders traditionally focused on characteristics such as yield or pest resistance, while breeding for crop quality, which largely depends on the presence and accumulation of highly valuable metabolites in the plant edible parts, was left out due to the complexity of plant metabolome and the impossibility to properly phenotype it. Recent technical advances in high throughput metabolomic, transcriptomic and genomic platforms have provided efficient approaches to identify new genes and pathways responsible for the extremely diverse plant metabolome. In addition, they allow to establish correlation between genotype and metabolite composition, and to clarify the genetic architecture of complex biochemical pathways, such as the accumulation of secondary metabolites in plants, many of them being highly valuable for the human diet. In this review, we focus on how the combination of metabolomic, transcriptomic and genomic approaches is a useful tool for the selection of crop varieties with improved nutritional value and quality traits.

Multi-omics approach to post-CABG renal impairment

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
C Boerschel ◽  
B Geelhoed ◽  
L Conradi ◽  
E Girdauskas ◽  
C Mueller ◽  
...  
Keyword(s):  
Renal Impairment ◽  
Leucine Zipper ◽  
Mixed Model ◽  
Artery Bypass ◽  
Bypass Graft ◽  
Control Cell ◽  
Integrated Approach ◽  
Cell Renewal ◽  
Gene Expressions ◽  
Tissue Samples

Abstract Background Renal impairment is a common complication after CABG (coronary artery bypass graft) surgery associated with an adverse outcome. Purpose To further characterize the molecular framework of the disease through omics analyses. Methods In N=165 CABG patients we performed multi-omics-analyses in preoperatively collected blood and tissue samples as well as 991 creatinine measurements. We used multivariable mixed-model regression analyses to analyse post-operative creatinine increase and to find common genetic polymorphisms, transcripts, metabolites and/or proteins associated with changes in postoperative creatinine increase. Multiple testing was accounted for by setting a 5%-limit on the false discovery rate (FDR) using the Benjamini-Hochberg procedure. Results Post-operative increase of log transformed creatinine was 0.035 (8%); 95% confidence interval (CI) 0.025, 0.045; P&lt;0.001. We identified 55 gene expressions and two proteins associated with post-CABG renal impairment. On the metabolomic and single nucleotide point mutation (SNP) level, no relevant targets were found. The three most important identified gene expressions were MIR3202.1 (beta of log transformed creatinine increase per standard deviation gene expression increase −0.034; 95% CI: −0.048, 0.020; P&lt;0.001), LOC105374386 (−0.032; 95% CI: −0.046, 0.019; P&lt;0.001) and maternal embryonic leucine zipper kinase (MELK) (−0.022; 95% CI: −0.032, 0.013; P&lt;0.001). Expression of all three was associated with a lower risk of post-CABG renal impairment. The same applies to the identified protein CAPRIN2 (−0.042; 95% CI: −0.062, 0.022; P&lt;0.001), while expression of the protein TUBB6 was associated with a higher risk (0.033; 95% CI: 0.017, 0.048; P&lt;0.001). Conclusions In an integrated approach we identified omics-biomarkers for the prediction of renal impairment after CABG surgery. The underlying pathophysiological associations of these genes and proteins are not fully understood. MELK might be an interesting target for further investigations, as it plays a prominent role in cell cycle control, cell proliferation, apoptosis, cell migration and cell renewal. Our results may help to better identify individuals at risk and lay the methodological groundwork for further omics analyses. Funding Acknowledgement Type of funding source: None

scholarly journals Home sperm testing device versus laboratory sperm quality analyzer: comparison of motile sperm concentration

2018 ◽  
Vol 110 (7) ◽  
pp. 1277-1284 ◽  
Author(s):  
Ashok Agarwal ◽  
Manesh Kumar Panner Selvam ◽  
Rakesh Sharma ◽  
Kruyanshi Master ◽  
Aditi Sharma ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Sperm Concentration ◽  
Testing Device ◽  
Motile Sperm ◽  
Quality Analyzer ◽  
Sperm Quality Analyzer

scholarly journals The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats

2017 ◽  
Vol 1 (1) ◽  
pp. 28
Author(s):  
Eviana Budiartanti Sutanto ◽  
Taufiq R Nasihun ◽  
Israhnanto Isradji ◽  
Luciana Budiati Sutanto
Keyword(s):  
Vitamin C ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Control Group ◽  
Control Group Design ◽  
Normal Sperm ◽  
Post Test ◽  
Male Wistar Rats ◽  
Post Hoc ◽  
Per Oral

Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in   K2 (K2  32.59  million/mL,  K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.

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