Effect of preovulatory follicle on fertility in Graded murrah buffaloes (Bubalus bubalis)

2017 ◽  
Author(s):  
M. Praveen Raj ◽  
G. Venkata Naidu ◽  
M. Srinivas ◽  
M. Raghunath ◽  
K. Ananda Rao
Keyword(s):  
Bubalus Bubalis ◽  
Preovulatory Follicle ◽  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Physiological Maturity ◽  
Murrah Buffaloes ◽  
Pregnancy Status ◽  
Follicle Size ◽  
Relationship Of ◽  
The Relationship

An investigation was taken up to study the relationship of preovulatory follicle size at the time of first postpartum AI and CL biometry with conception using transrectal ultrasonography in 70 parous Graded Murrah buffaloes maintained under rural conditions. POF sizes were measured ultrasonographically and grouped in to small preovulatory follicle (SPF), medium preovulatory follicle (MPF) and large preovulatory follicle (LPF). Serum progesterone concentration at the time of AI and pregnancy status was negatively correlated indicating that when progesterone level declined to less than 0.3 ng/ml (basal level) at the time of AI, the chances of the animal becoming pregnant was increased. The size of the POF was positively correlated to the size of the CL on day 10 in pregnant buffaloes. The overall POF diameter at the time of estrum was 12.31 ± 0.29 mm (Range 9 to 16 mm) in Graded Murrah buffaloes and 50 % of the buffaloes had POF size > 12 - £14 mm. They conceived only if the POF diameter was more than 9 mm at the time of AI however there was no significant correlation between the POF size at the time of AI and conception. Thus, it was concluded that physiological maturity rather than the diameter of the follicle influenced the fertility in Graded Murrah buffaloes under field conditions.

Superovulatory Response and Progesterone Profile in Murrah Buffaloes (Bubalus bubalis) Pretreated with GnRH Agonist

2020 ◽  
Author(s):  
Sumit Singhal ◽  
Shiv Prasad ◽  
Rajesh Verma ◽  
H.P. Gupta ◽  
J.K. Prasad
Keyword(s):  
Embryo Transfer ◽  
Bubalus Bubalis ◽  
Hormone Concentration ◽  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Group Iii ◽  
Embryo Recovery ◽  
Murrah Buffaloes ◽  
Multiple Ovulation ◽  
Group I

Background: Limited elite buffalo population need to be propagated at faster rate to sustain the dairy industry in India. Faster propagation could be achieved through embryo transfer technology. Low serum progesterone concentrations during the start of superstimulatory treatment is major factor that hampers the success of multiple ovulation embryo transfer (MOET) in buffaloes. This study evaluated the effect of GnRH pretreatment before superovulatory regimen on progesterone profile, superovulation and correlation of progesterone with superstimulatory parameter in Murrah buffaloes. Methods: Buffaloes (n=27) were superstimulated using 600 mg Folltropin and divided into three groups on basis of pretreatment: Group I and group II were pretreated with GnRH @ 10 µg and 06 µg, respectively while no pretreatment was subjected in group III (control). Progesterone hormone concentration, superovulatory response, ovulation rate, embryo recovery were recorded. Result: Our study revealed that superovulatory response was non-significantly different in all three groups and ranged from 6-8 in terms of number of corpus luteum (CL). Average number of recovered embryos in this study was nearly 2.0. A positive correlation between progesterone concentrations at the initiation of FSH treatment with number of CL and embryo recovery was observed. The study indicated that GnRH pretreatment before superstimulation improve the serum progesterone concentration, superovulatory response and embryo recovery in Murrah buffaloes. 

scholarly journals Prediction of Reproductive Success in Multiparous First Service Dairy Cows by Parameters from In-Line Sensors

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 334
Author(s):  
Ramūnas Antanaitis ◽  
Vida Juozaitienė ◽  
Dovilė Malašauskienė ◽  
Mindaugas Televičius ◽  
Mingaudas Urbutis ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Blood Parameters ◽  
Negative Energy ◽  
Hydroxybutyric Acid ◽  
Pregnancy Status ◽  
Group A ◽  
Biochemical Indices ◽  
Relationship Of ◽  
The Relationship

The aim of the current study was to evaluate the relationship of different parameters from an automatic milking system (AMS) with the pregnancy status of multiparous cows at first service and to assess the accuracy of such a follow-up with regard to blood parameters. Before the insemination of cows, blood samples for measuring biochemical indices were taken from the coccygeal vessels and the concentrations of blood serum albumin (ALB), cortisol, non-esterified fatty acids (NEFA) and the activities of aspartate aminotransferase (AST) and gamma glutamyltransferase (GGT) were determined. From oestrus day to seven days after oestrus, the following parameters were registered: milk yield (MY), electric milk conductivity, lactate dehydrogenase (LDH) and β-hydroxybutyric acid (BHB). The pregnancy status was evaluated using ultrasound “Easy scan” 30–35 days after insemination. Cows were grouped by reproductive status: PG− (non-pregnant; n = 48) and PG+ (pregnant; n = 44). The BHB level in PG− cows was 1.2 times higher (p < 0.005). The electrical conductivity of milk was statistically significantly higher in all quarters of PG− cows (1.07 times) than of PG+ cows (p < 0.05). The arithmetic mean of blood GGT was 1.61 times higher in PG− cows and the NEFA value 1.23 times higher (p < 0.05) compared with the PG+ group. The liver function was affected, the average ALB of PG− cows was 1.19 times lower (p < 0.05) and the AST activity was 1.16 times lower (p < 0.05) compared with PG+ cows. The non-pregnant group had a negative energy balance demonstrated by high in-line milk BHB and high blood NEFA concentrations. We found a greater number of cows with cortisol >0.0.75 mg/dL in the non-pregnant group. A higher milk electrical conductivity in the non-pregnant cows pointed towards a greater risk of mastitis while higher GGT activities together with lower albumin concentrations indicated that the cows were more affected by oxidative stress.

80 RECIPIENT SERUM PROGESTERONE CONCENTRATION AND EARLY CONCEPTUS DEVELOPMENT IN BOVINE EMBRYOS PRODUCED IN VIVO OR IN VITRO

2012 ◽  
Vol 24 (1) ◽  
pp. 152
Author(s):  
C. V. Barnwell ◽  
C. S. Whisnant ◽  
C. E. Farin ◽  
J. E. Alexander ◽  
P. W. Farin
Keyword(s):  
State University ◽  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Exact Test ◽  
Treatment Groups ◽  
Conceptus Development ◽  
Development Outcomes ◽  
The Relationship

The majority of pregnancy loss in cattle occurs during the first 2 to 3 weeks of pregnancy. This loss can be studied by the transfer of in vivo- and in vitro-produced embryos. The objective of this study was to examine the relationship between recipient serum progesterone levels both at the time of embryo transfer and at conceptus recovery on conceptus development from in vivo- or in vitro-produced embryos. Embryos were produced in vivo by superovulation of Holstein cows (IVO; n = 17) or in vitro with either serum-containing (IVPS; n = 27) or serum-restricted medium (IVPSR; n = 34). Single grade-1 blastocysts from each embryo production system were transferred into heifers at Day 7. Conceptuses were recovered at Day 17 of gestation (number recovered/number transferred: IVO, 11/17; IVPS, 16/27; IVPSR, 18/34) and classified as complete, degenerated, or no conceptus. Recipient serum progesterone concentrations were determined by radioimmunoassay and compared with conceptus development outcomes. Sex of conceptus was determined by PCR using a Y-chromosome specific probe. Data were analysed using Fisher's exact test or ANOVA and Duncan's multiple range test. Compared with the IVO group, in vitro-produced embryos had more (P = 0.055) degenerated conceptuses (IVO, 0%; IVPS, 18.5%; IVPSR, 20.6%). There were no differences (P > 0.05) in serum progesterone concentrations in recipients assigned to different treatments at Day 7. There was also no effect (P > 0.05) of treatment on progesterone levels in recipients with either male or female conceptuses at the time of transfer. Interestingly, heifers in the in vitro treatment groups had lower (P < 0.01) progesterone concentrations at Day 7 when no conceptus was recovered at Day 17 (IVPS, 2.1 ± 0.4 ng mL–1; IVPSR, 2.7 ± 0.4 ng mL–1; Least squares means ± standard error of the mean) compared with the IVO group (4.5 ± 0.6 ng mL–1). There was no difference in progesterone concentration between treatment groups for heifers with shorter conceptuses (≤194 mm). However, when longer (>194 mm) conceptuses were recovered, heifers with in vitro produced embryos had lower (P < 0.05) progesterone levels at Day 7 compared with those with in vivo produced embryos (IVPS, 2.2 ± 0.6 ng mL–1; IVPSR, 2.3 ± 0.5 ng mL–1; IVO, 3.9 ± 0.6 ng mL–1). In summary, serum progesterone concentrations in recipients at the time of transfer of in vivo- or in vitro-produced embryos were associated with conceptus development at Day 17 of gestation. Research supported by NC State University GAANN Biotechnology Fellowship (C. V. Barnwell) and the College of Veterinary Medicine.

Changes in Teat Morphology (Doka Phenomenon) and Estrus Prediction in Riverine Buffaloes (Bubalus bubalis)

2019 ◽  
Author(s):  
S K Joshi ◽  
T K Mohanty ◽  
A Kumaresan ◽  
M Bhakat ◽  
S Sathapathy
Keyword(s):  
Morphological Changes ◽  
Bubalus Bubalis ◽  
North India ◽  
Research Centre ◽  
Murrah Buffaloes ◽  
Group I ◽  
Before And After ◽  
Group Ii ◽  
The Difference ◽  
The Relationship

The objective of the study was to characterize the term Doka and to capture morphological changes in teat to find out the relationship between Doka and onset of estrus in Murrah buffaloes (Bubalus bubalis). The Murrah buffaloes maintained at of Livestock Research Centre (LRC), ICAR-NDRI, Karnal were classified into two groups based on the lactation number for the study of Doka characteristics. In group I, twenty animals up to 3rd lactation were incorporated, whereas thirty animals from 4th to 5th lactation were included in group II. The period when buffaloes showed teat engorgement was called as Doka period, is the local term used by the farmers in North India that can be taken as a visual sign for prediction of estrus. The length and diameter of teats, before and after milking were measured, for both the groups during morning and evening milking and subsequently analyzed. The present study confirmed that the teat diameter (mm) before milking during Doka was significantly (plessthan0.05) higher as compared to pre Doka period in both the groups (group-I - 37.01 ±2.13 vs 28.42 ±1.64, group-II - 53.49 ±2.82 vs 41.096±2.16). The teat diameter (mm) during Doka period after milking was significantly (plessthan0.05) more in both the groups (group-I - 26.65 ±1.26 vs 24.44 ±1.16, group-II - 39.27 ±2.41 vs 35.31 ±2.17) as compared to pre Doka period. So, it can be concluded that the changes in teat diameter can safely be considered as a sign of Doka and the forthcoming heat in Murrah buffaloes. However, the difference in teat length between different stages of Doka was found to be non significant.

Studies on Progesterone Concentration and Fertility Response in Postpartum Subestrus Buffaloes during Breeding and Low Breeding Seasons

2021 ◽  
Author(s):  
B. Chandra Prasad ◽  
G. Venkata Naidu ◽  
M. Srinivas ◽  
M. Raghunath ◽  
Ashwini Kumar
Keyword(s):  
Breeding Season ◽  
Bubalus Bubalis ◽  
Pregnancy Rates ◽  
Progesterone Concentration ◽  
Murrah Buffaloes ◽  
Farming Community ◽  
Livestock Sector ◽  
Gross Income ◽  
Breeding Seasons ◽  
Reproductive Problem

Background: The dairy and livestock sector plays a very important role in national economy of India by contributing close to one third of gross income of rural households and nearly half of gross income in case of those without land. Subestrus is the most prevalent, frustrating and challenging problem encountered in postpartum buffaloes. Postpartum subestrus is one of the most prevalent, frustrating and challenging reproductive problem encountered in rural resulting into prolonged inter-calving period, reduced milk production and thus greatly affecting the economy of our farming community. Methods: The present study was carried out on Graded Murrah buffaloes during the period from September 2017 to August 2019 (includes breeding and low breeding season) which were maintained at farm and field. Treatment of sub-estrus lactating graded Murrah buffaloes (Bubalus bubalis) using Double PGF2 α (DPG) and Presynch-Ovsynch (POVS) their influence on fertility in farm and field condition during breeding and low breeding season was studied. Total cholesterol and progesterone concentration was recorded during different days/period of protocols. Further, overall per cent conception and pregnancy rates were recorded and compared between two protocols. Result: Progesterone levels increased significantly on day 21 post AI in pregnant buffaloes, whereas decreased significantly in non-pregnant buffaloes both in farm and field during breeding and low breeding season. The overall per cent conception and pregnancy rates were higher in POVS group than DPG group at both farm and field as well as during breeding and low breeding seasons.

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