Effect of dietary amino acid regimens on growth performance and body conformation and immune responses in Aseel chicken

Author(s):  
Munawar Hussain ◽  
Athar Mahmud ◽  
Jibran Hussain ◽  
Shafqat Nawaz Qaisrani ◽  
Sohail Ahmad ◽  
...  

The present study was designed to evaluate the effect of different levels of Lysine (Lys) on early growth, body conformation and immune response of three varieties of Aseel chicken. Five hundred and forty day old chicks were used in this experiment. Experimental treatments were three verities i-e Mianwali (MW), Peshawari (PW), and Lakha (LK) and three level of Lys i-e L1 (1.35), L2 (1.30) and L3 (1.25%). Treatments were arranged in factorial arrangement under randomized complete block design (RCBD) with sex as block. Each experimental group was replicated 6 times with 10 birds in each. Results indicated that PW variety showed a greater feed intake (FI) throughout the experiment, however, body weight gain (BWG) and feed conversion ratio (FCR) were remained unchanged within varieties. Lower FI was observed in birds fed diets containing 1.35, 1.30% digestible Lys. Birds fed diets containing 1.35, 1.30% digestible Lys showed higher (BWG (P less than 0.05) and improved FCR (P less than 0.05). Mianwali variety with 1.35, 1.30% digestible Lys diets, showed (252.0±17.4 and 251.4±7.15; 2.37±0.17 and 2.35±0.06) an interaction for BWG and FCR. Birds fed amino acid dense diets depicted improved body conformation traits (length of body, drum stick, shank keel bone, circumference of drumstick and shank, wing spread and breast width), whereas immune responses (were not influenced by the dietary treatments. In conclusion, it was found that 1.30% digestible Lys level with ideal amino acid ratio was better for improving early BWG and FCR in Mianwali Aseel.

2021 ◽  
Vol 45 (1) ◽  
pp. 27
Author(s):  
Engkus Ainul Yakin ◽  
Sri Sukaryani ◽  
Catur Suci Purwati

This experiment was aimed to evaluate the effect of fermented cocoa pod as feed for Bligon goats. Sixteen Bligon goat with a body weight of 11-13 kg were put into individual cages which were equipped with feed and drink containers. The study design used a randomized block design, where initial body weight in as peragam with four treatments and four replications. Fermented cocoa pod used Trametes versicolor. The experimental treatments were T1= 30% of fresh king grass + 50% of dried king grass + 20% of concentrate; T2= 30% of fresh king grass + 30% of dried king grass + 40% of concentrate; T3= 30% of fresh king grass + 30% of cocoa pod + 40% of concentrate; and T4= 30% of fresh king grass + 30% of cocoa pod fermented + 40% of concentrate. Observed variables were feed intake, body weight gain and feed conversion. The result showed that fermented cocoa pod at the level of 30% had higher (P<0.05) infeed intake (560.33 g day-1), body weight gain (101.79 g head-1day-1), and feed conversion (5.50) compared to other treatments. The conclusion of this study were the use of 30% cocoa pod fermented in the ration showed the best body weight gain and feed conversion on Bligon goat performance. 


2021 ◽  
Vol 9 (6) ◽  
pp. 1341
Author(s):  
Sarayu Bhogoju ◽  
Collins N. Khwatenge ◽  
Thyneice Taylor-Bowden ◽  
Gabriel Akerele ◽  
Boniface M. Kimathi ◽  
...  

There are well documented complications associated with the continuous use of antibiotics in the poultry industry. Over the past few decades, probiotics have emerged as viable alternatives to antibiotics; however, most of these candidate probiotic microorganisms have not been fully evaluated for their effectiveness as potential probiotics for poultry. Recent evaluation of a metagenome of broiler chickens in our laboratory revealed a prevalence of Lactobacillus reuteri (L. reuteri) and Actinobacteria class of bacteria in their gastrointestinal tract. In this study Lactobacillus reuteri and Streptomyces coelicolor (S. coelicolor) were selected as probiotic bacteria, encapsulated, and added into broiler feed at a concentration of 100 mg/kg of feed. In an 8-week study, 240 one day-old chicks were randomly assigned to four dietary treatments. Three dietary treatments contained two probiotic bacteria in three different proportions (L. reuteri and S. coelicolor individually at 100 ppm, and mixture of L. reuteri and S. coelicolor at 50 ppm each). The fourth treatment had no probiotic bacteria and it functioned as the control diet. L. reuteri and S. coelicolor were added to the feed by using wheat middlings as a carrier at a concentration of 100 ppm (100 mg/kg). Chickens fed diets containing L. reuteri and S. coelicolor mixture showed 2% improvement in body weight gain, 7% decrease in feed consumption, and 6–7% decrease in feed conversion ratios. This research suggests that L. reuteri and S. coelicolor have the potential to constitute probiotics in chickens combined or separately, depending on the desired selection of performance index.


Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2416
Author(s):  
Reza Barekatain ◽  
Tristan Chalvon-Demersay ◽  
Clive McLaughlan ◽  
William Lambert

Two experiments were conducted to investigate the effect of arginine (Arg); the combination of Arg and glutamine (Gln); as well as an amino acid-based solution (MIX) containing Arg, Gln, threonine (Thr), and grape extract, on performance, intestinal permeability, and expression of selected mechanistic genes. Using 240 male Ross 308 off-sex broiler chickens, four experimental treatments were replicated six times with 10 birds per replicate. The experimental treatments included 5 g/kg Arg, 2.5 g/kg Arg and 2.5 g/kg Gln, and 1 g/kg MIX added to a basal diet as control. In the second study, the four dietary treatments were then given to 24 birds with or without a synthetic glucocorticoid, dexamethasone (DEX), as a gut dysfunction model. Feed conversion ratio was improved by all the supplemented treatments from day 7 to 35 of age (p < 0.001). DEX injections increased (p < 0.001) the intestinal permeability in all treatments, which tended to be reversed by Arg or MIX. Additional Arg, Arg-Gln, and MIX suppressed (p < 0.05) the overexpression of IL-1β generated by DEX. Feeding birds with MIX treatment increased (p < 0.05) expression of SGLT-1 and glutathione synthetase. In conclusion, tested amino acid supplements were effective in improving feed efficiency and restraining intestinal inflammation caused by DEX through IL-1β pathway.


Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 889 ◽  
Author(s):  
Jacek Trela ◽  
Bartosz Kierończyk ◽  
Veerle Hautekiet ◽  
Damian Józefiak

The aim of the study was to investigate the effect of Bacillus licheniformis and salinomycin supplementation in broiler diets as individual factors or in combination on the growth performance, GIT morphometry, and microbiota populations. Four hundred one-day-old Ross 308 chicks were randomly distributed to four dietary treatments (10 replicates, 10 birds each). The following treatments were applied: NC—no additives; NC + SAL—salinomycin addition (60 mg/kg diet), NC + PRO—B. licheniformis DSM 28710 preparation (1.6 × 109 CFU/kg; 500 mg/kg diet), and NC + SAL + PRO—combination of salinomycin and B. licheniformis. Probiotic administration resulted in improvement (p < 0.05) of the performance parameters, including body weight gain (1–10 d, and 11–22 d) and feed conversion ratio (11–22 d, 1–36 d). An interaction (p < 0.05) between experimental factors was observed in terms of lower pH values in the crop (tendency, p = 0.053) and ceca. Both factors lowered the alpha diversity and Enterobacteriaceae and promoted Bacillaceae communities in the jejunum (p < 0.05). Interactions were also observed in terms of reducing Clostridiaceae in the ceca. In conclusion, the combined use of B. licheniformis and salinomycin in broilers’ diets had beneficial effects.


2019 ◽  
Vol 3 (4) ◽  
pp. 1112-1118
Author(s):  
Christopher Antwi ◽  
Patience A Mensa ◽  
Fareed K N Arthur ◽  
Yaw O Frimpong ◽  
John Baah

Abstract A 4 mo feeding trial was conducted to ascertain the effect of direct-fed microbial (DFM) and their products, namely rumen enhancer three (RE3), Paenibacillus polymyxa (P3), and a fermentation product of RE3—RE3 Plus on the growth and haematological profile of lambs at different stages of growth (suckling, weaner, and grower phases). The lambs weighing 2.5 ± 2 kg were blocked by their weights and allotted to four dietary treatments, namely Control, RE3, RE3 Plus, and P3 in a randomized complete block design. Blood sampling of lambs to investigate the effect of the treatments on the haematological and blood biochemistry variables was done on monthly basis and analysis of variance in a repeated measures design was done using the Statistical Analysis System. Feed intake (FI) by lambs was not significantly (P &gt; 0.05) influenced by DFM supplementation for all the phases of growth. However, feed conversion ratio (FCR) for the grower phase of the lamb was higher for T2. Similar (P &gt; 0.05) hemoglobin (Hb), red blood cell (RBC), pack cell volume (PCV), mean corpuscular volume, mean corpuscular hemoglobin concentration (MCHC), eosinophil, lymphocyte, and basophil composition were recorded for lambs on the different experimental diets. Neutrophil and monocytes levels were, however, different (P &lt; 0.05) for lambs fed the varying dietary treatments with lambs on RE3 Plus diets recording the lowest (P &lt; 0.05) neutrophil levels. Monocytes levels were highest (P &lt; 0.05) in lambs fed diets fortified with RE3. Sampling period influenced (P &lt; 0.05) the Hb, RBC, PCV, MCHC, eosinophil, monocyte, and lymphocyte levels. The trial revealed a significant treatment × sampling period interaction (P &lt; 0.0001) for the blood parameters examined. The inclusion of the different DFM products had no influence on the growth characteristics and blood profile of growing lambs.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 74-74
Author(s):  
M I Gracia Lorenzo ◽  
Yves Mercier ◽  
O Casabuena Rincon ◽  
Jaime Sanchez Laguna ◽  
Dolores I Batonon-Alavo

Abstract This work aimed to compare piglet’s performance when fed DL-Methionine or Hydroxy-Methionine (OH-Met and OH-MetCa) at the requirements of sulfur amino acids (SAA) or above. 720 piglets (♀ large white*landrace x ♂ pietrain*duroc) were weaned at 28 days old (6.84 ± 1.07 kg) and randomly allocated to the experimental treatments for 42 days. The experiment was a factorial design with three Met sources (DL-Met, liquid OH-Met (acid form) or OH-MetCa under powder form) and two doses of SAA. Treatments were formulated to reach the adequate SID SAA/Lys ratio of 58% or exceeded the Spanish nutrient recommendations in SAA by 25% (0.79% SID SAA/Lys). Each treatment was replicated 12 times (6 pens of males and 6 pens of females). Growth performance was measured and data was analysed by mixed-effects models (R Core Team). The body weight was neither affected by SAA level, nor by the Met sources. The daily weight gain (DWG) was not affected by the dietary treatments from 0 to 21 days; whereas the feed conversion ratio (FCR) was significantly (P=0.01) improved when the SAA level was increased by 25% (1.272 and 1.184) due to the decreased feed intake (P=0.03). Significant interactions were found between the Sex and the SAA level for DWG (P = 0.04) and FCR (P = 0.03) from 0 to 21 days. From day 21 to 42 and from day 0 to 42, no performance criteria were different in all dietary treatments. For all criteria of performance, the three Met sources exhibited a similar response. These results confirmed that Met sources are equivalent in piglets.


ZOOTEC ◽  
2020 ◽  
Vol 40 (1) ◽  
pp. 352
Author(s):  
Sony A.E. Moningkey ◽  
R.A.V. Tuturoong ◽  
I.D.R. Lumenta

UTILIZATION OF FERMENTED RUMENT CONTENT WITH CELLULOMONAS SP IN MIXED COMPLETE FEED FOR RABBIT. Research conducted to learn how to use cattle rumen content by using fermentation processing techniques to enable this rumen to be used as rabbit feed. The material used in this study consisted of cattle rumen contents, starter Cellulomonas sp, rabbits, complete feed. This research consisted of two phase. The first study used an experimental method with a completely randomized design  4 preparations and 6 replications. The fermentation time consists of 0 hours, 24 hours, 48 hours and 72 hours. For the second study using an experimental method with randomized block design  based on the initial body weight of rabbits. The treatment given is the level of use of the best fermented rumen contents in a complete feed ration. Variable which is translated as feed consumption, body weight gain and feed conversion. Research results The first stage of the P4 study sample (72 hours) as the best guideline is seen from the parameters of crude protein and crude fiber. The results of this study indicate that the use of feed using rumen fermentation (IRF) can increase feed consumption and weight gain. The conclusion of this study is the provision of 30% mixture of fermented rumen contents of Cellulomonas sp in complete feed produced the best results seen from the parameters of consumption, weight gain and feed conversion of rabbit.Keywords: Rumen contents, fermentation, Cellulomonas sp, complete feed, rabbits


1970 ◽  
Vol 10 ◽  
pp. 29-35 ◽  
Author(s):  
T. B. Rijal ◽  
D. B. Nepali ◽  
R. A. Sah ◽  
M. P. Sharma

An experiment was conducted from 18th October, 2006 to 18th January, 2007 at IAAS Livestock Farm, Rampur, Chitwan to determine the substitution effects of soybean meal with brewer's dried grain (BDG) on the performance, economics and meat traits of swine production. Starter, grower and finisher diets were formulated containing 18, 16, and 14% CP respectively with 3000 Kcal/kg ME and adequately supplemented with required vitamins, minerals and amino acids. The treatments were: diet without brewer's dried grain -T1 (control diet), diet substituted with 10% brewer's dried grain-T2, diet substituted with 20% brewer's dried grain-T3, diet substituted with 30% brewer's dried grain-T4 and diet substituted with 35% brewer's dried grain-T5. Twenty weaned piglets having 7.37 to 7.94 kg live weight were housed in individual pens. Five piglets were randomly assigned to the five treatments following a randomized complete block design (RCBD) considering each individual as a replication. Fortnightly live weight, daily feed consumption, income over feed and pig cost, and different meat traits of pigs were recorded. The effect of replacing formulated diets with various level of brewer's dried grains on body weight gain was significant (p<0.01) with higher level of substitution resulting lower live-weight at the end of each of starter, grower and finisher diet period. The average body weight of piglets substituted with 35%BDG (27.00kg) and 30%BDG (39.75kg) were significantly lower (p<0.01) compared to the weight of piglets receiving 0% (65.75kg), 10% (64.25kg) and 20% (60.25kg) BDG replaced diets at the end of 3 months experimental period. A significant reduction (P<0.01) in average daily feed consumption were also recorded with increasing level of BDG in the diet. Better feed conversion ratio (FCR) was observed in T2 at early period which at finisher stage T3 diet resulted significantly better FCR which was at par with T1 and T2 diet fed group. However, T5 diet resulted the poorest feed conversion ratio of 5.26, 4.92 and 5.87 for starter, grower and finisher respectively. There was reduction in feed cost with different levels of BDG substitution in diet of pig. Highest income (in per cent) over expenditure (109.01%) was found in T2 followed by T1, T3, and T4. Lowest income over expenditure (-8.2%) was found in T5. Highest dressing percent (71.87%) was recorded in T1 and lowest (70%) in T5 diet fed group. Inclusion of 10-20% BDG seems most acceptable level of substitution of SBM. However, this result needs to be verified under farmer's management condition before recommendation.Key words: Brewer's dried grains; Swine performanceDOI: 10.3126/njst.v10i0.2820 Nepal Journal of Science and Technology Volume 10, 2009 December Page: 29-35  


2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Mojtaba Roudgar Amoli ◽  
Kaveh Jafari Khorshdidi ◽  
Mohammad Jafari

This experiment was conducted to investigate the effect of rosemary powder in diet on blood metabolite and performance of broiler chickens. 180 one day broiler chicks were distributed in a completely randomize design with 4 treatments and 3 replicates and 15 bird in each. Experimental treatments were included: Control (without additive), 0.05, 0.1 and 0.2 percent of rosemary in diet. In 42 day of age one bird from each replicate was selected and slaughtering for carcass traits. The results were sowed use of 0.05 percent of rosemary could be significantly increased feed intake of broiler in starter period (p<0.05). Also use of 0.1 percent of rosemary caused to significantly increase broiler body weight gain in overall of rearing period than 0.2 percent group (p<0.05). Use of 0.1 percent rosemary in diet had a significantly  feed conversion ratio (FCR) than T4 (0.2% rosemary). Experimental treatments had no significant effect on carcass traits, except in liver percent weight, which had significantly higher in T2 group (p<0.05). Also experimental treatments had no significant effect on glucose, cholesterol, triglyceride level of broiler chickens in 42 day of age. The results of this experiment showed use of rosemary powder could be improved broiler feed intake in starter period.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 351-352
Author(s):  
S-M Hong ◽  
D Jung ◽  
S-J You

Abstract A body of evidence suggests that low-protein diets in swine have benefits in view of environmental stewardship and economic returns. The present study was performed to evaluate essential amino acid (EAA) supplementation in the diets with reducing crude protein (CP) concentrations on growth performance in fattening pigs. We hypothesized that a low-CP diet with elevated EAA concentrations would result in similar growth performance in fattening pigs compared with a normal CP diet. Eighty finishing pigs with initial body weight (BW) of 72.0 ± 5.34 kg were assigned in a randomized complete block design with 5 replicates to test 4 dietary treatments: 1) 15.5% CP with EAA concentration at 109% of NRC requirement (control); 2) 14.0% CP with EAA concentration at 111% of NRC requirement (EAA111); 3) 14.0% CP with EAA concentration at 113% of NRC requirement (EAA113); and 14.0% CP with EAA concentration at 115% of NRC requirement (EAA115). The diets were isocaloric, whereas crystalline amino acids, L-Lys, L-Met, L-Thr, L-Trp, and L-Val were added to the diets to achieve the varying levels of EAA in the treatments. The Lys-to-net energy ratio in the diets was maintained at 3.06, 3.12, 3.18, and 3.24 to the control, EAA111, EAA113, and EAA115, respectively. Dietary treatments did not affect final BW. Additionally, average daily gain, average daily feed intake, and feed conversion ratio (FCR) were similar across the treatments. On average, pigs fed the control and EAA111 numerically decreased FCR compared to those fed EAA113 and 115 (3.21 vs. 3.35, respectively; P = 0.46). Total feed cost throughout the experiment was $9.0/head lessen in EAA111 relative to the control; however, total feed costs to EAA113 and EAA115 were greater than the control. In summary, a low-CP diet with increased EAA supplementations did not have any negative impact on growth performance of fattening pigs.


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