Histopathological alterations of selenium toxicity induced in broiler (Birds)

2017 ◽  
Author(s):  
Avnish Kumar Gautam ◽  
Deepak Kumar ◽  
Manoj Kumar Sinha
Keyword(s):  
Drinking Water ◽  
Toxic Effect ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Sodium Selenite ◽  
Biochemical Parameter ◽  
Lymphoid Cells ◽  
Bursa Of Fabricius ◽  
White Leghorn ◽  
Group A

A study was conducted to determine the pathological toxic effect of selenium (Sodium selenite). A total number 120 one day old White Leghorn (WLH) healthy broiler birds were randomly divided into A, B and C groups. Each group contain 40 birds daily administration of sodium selenite @ 30ppm and @ 15 ppm in group A and B, respectively and group C was given plain drinking water daily for 42 days and kept as control. Macroscopically and microscopically revealed varying degrees of congestion and haemorrhages in lungs, liver, kidneys, heart and intestine in selenium treated birds. The bursa of Fabricius showed depletion of lymphoid cells between the interfollicular spaces. A significant (P less than 0.05) decrease in the haemoglobin and packed cell volume was noticed in both the selenium fed groups but the total erythrocyte count remain unchanged. Biochemical parameter revealed slight decline in their activity of serum ALT and AST and increased level of BUN and creatinine in group A and B as compared to group C, suggesting some degree of renal dysfunctioning.

scholarly journals Quantitative Characterization of Chick Thymus and Bursa Development

Blood ◽  
1966 ◽  
Vol 27 (3) ◽  
pp. 371-379 ◽  
Author(s):  
JACK SHERMAN ◽  
ROBERT AUERBACH
Keyword(s):  
Cell Volume ◽  
Critical Size ◽  
Lymphoid Cells ◽  
Bursa Of Fabricius ◽  
Size Analysis ◽  
Quantitative Characterization ◽  
Cell Size Distribution ◽  
Mean Cell Volume ◽  
Morphogenetic Change

Abstract 1. Critical size analysis of the lymphoid cell populations of the developing chick bursa of Fabricius and thymus indicates that characteristic shifts in cell size distribution, modal cell volume and mean cell volume occur during morphogenesis of these organs. 2. Comparison of bursa and thymus shows that while there are clear differences between the lymphoid cells of these two structures, the pattern of morphogenetic change is similar. 3. 19-nortestosterone injected in low doses can partially inhibit bursal development without affecting thymus morphogenesis. Depending on the age of the embryo at time of injection, however, doses sufficient to produce complete bursal inhibition may also retard thymic morphogenesis. 4. The size differences found between bursal and thymic lymphocytes in the chicken parallel the size differences found between lymph node and thymic lymphocytes in the mouse.

scholarly journals Effect of Physical Exercise on some Hematological Parameters in Female Athletes in Bangladesh

1970 ◽  
Vol 52 (195) ◽  
pp. 892-896 ◽  
Author(s):  
Tanbira Alam ◽  
Lubna Shirin ◽  
SM Niazur Rahman ◽  
Tanjeda Alam ◽  
Nasrin Habib ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Serum Iron ◽  
Female Athletes ◽  
Ferritin Level ◽  
Hematological Parameters ◽  
Control Group ◽  
Group A ◽  
Group B

Introduction:  Long term physical training has been considered to adversely affect the performance of athletes especially the females. It may be due to the iron depletion caused by hemolysis or hemodilution results from plasma volume expansion. This study aims to assess the effect of heavy exercise on hemoglobin concentration and some other hematological parameters in female athletes. Methods: This cross sectional study was carried out in the Department of physiology, Dhaka Medical College, Dhaka, from July 2010-June 2011. A total number of 105 females (70 athletes and 35 non athletes) between 15-25 years of age were recruited. The 35 apparently healthy female non athletes were taken as control (Group-A) and 70 female athletes comprise the study group (Group-B). According to sporting category, Group-B was further subdivided as B1:Runners and B2:Cyclists. Hemoglobin percentages (Hb %), red blood cell (RBC) concentration, packed cell volume (PCV), serum iron, serum ferritin and total iron binding capacity (TIBC) were measured in fasting state after two months training. Statistical analysis was done by using SPSS/ win.15.0 for comparison between the groups, using student’s unpaired “t” test. Results: Mean Hb%, RBC, PCV, iron and ferritin level were significantly (P<0.001) lower in athletes than those of control group. Within the study groups, runner’s serum iron and ferritin level were lower than cyclists.  TIBC significantly (P<0.001) rose in Group-B2 in comparison to Group-A. Conclusions: The current study showed that intense physical exercise leads to early stages of Hb%, RBC, PCV, iron and ferritin depletion which might compromise the health and performance of athletes. Keywords: exercise; hemoglobin; packed cell volume; serum iron.  

scholarly journals Studies on effects of lactose on experimental Trypanosoma vivax infection in Zebu cattle. 2. Packed cell volume

2008 ◽  
Vol 75 (3) ◽  
Author(s):  
M.Y. Fatihu ◽  
S. Adamu ◽  
I.A. Umar ◽  
N.D.G. Ibrahim ◽  
L.O. Eduvie ◽  
...  
Keyword(s):  
Cell Volume ◽  
Normal Saline ◽  
Packed Cell Volume ◽  
Rate Of Change ◽  
Zebu Cattle ◽  
Trypanosoma Vivax ◽  
Rates Of Change ◽  
Group A ◽  
The Mean ◽  
Infusion Period

The ability of intravenously administered lactose in normal saline to prevent a decline in packed cell volume (PCV) during experimental trypanosomosis was studied in Zebu cattle. During the lactose infusion period, the PCV was stable up to Day 5 post-infection (p.i.) in a lactose-infused group, compared to that in an uninfused group in which the PCV dropped significantly (P < 0.05) as shown by the values of cumulative percentage change. Furthermore the mean rate of change in PCV was significantly (P < 0.05) higher in the uninfused group relative to the lactose-infused group during the same period. While the PCV fell markedly in the lactose-infused group a day after lactose infusion was stopped (Day 13 p.i.), subsequent PCV values were significantly (P < 0.05) higher compared to those in the uninfused group, up to the end of experiment on Day 17 p.i. However the mean rates of change in PCV did not vary significantly (P > 0.05) between the groups during the period in which lactose infusion was stopped. The mean levels of parasitaemic waves and parasitaemia were higher, more prolonged and more frequent in the lactose-infused group. It was inferred that the lactose was able to prevent an early onset of anaemia in the Trypanosoma vivax-infected Zebu cattle.

scholarly journals Discriminate and indiscriminate use of amoxicillin and its effects on hematological parameters of broiler

2019 ◽  
Vol 5 (2) ◽  
pp. 153-157
Author(s):  
Md Shakil Islam ◽  
Md Zahorul Islam ◽  
Md Shafiqul Islam
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Hematological Parameters ◽  
Control Group ◽  
Broiler Chicks ◽  
Blood Parameter ◽  
Withdrawal Period ◽  
Significant Difference ◽  
Group A ◽  
Group B

Hematological indication is an important finding for pathophysiology analysis of biological science. This study was designed with the aim to investigate the effects of residual antibiotics on hematological parameters of broiler following discriminate and indiscriminate use. Broiler chicks were reared accordingly upto 30 days and on day 14 the chicks were randomly divided into 3 groups (n=6) namely control (group A), discriminate (group B) and indiscriminate (group C). At the age of day 16, amoxicillin treatment was started and continued for seven (7) days for in discriminate group (Group B) and fifteen (15) days for indiscriminate group. In case of discriminate group withdrawal period was properly maintained as nomenclature (Seven days) whereas, there was no withdrawal period in case of indiscriminate group. The hemoglobin (gm%) of control, discriminate, and indiscriminate group was 7.07±0.099, 6.97±0.095, and 6.90±0.124; Total erythrocyte count (TEC) 2.55±0.044 million/mm3, 2.53±0.038 million/mm3, and 2.50±0.026 million/mm3; Packed Cell Volume PCV (%) 19.83±1.302, 19.33±1.054, and 18.50±0.764 respectively. There was no significant difference on blood parameter found among the groups. Therefore, discriminate and indiscriminate use of amoxicillin has no bad effect on hematological parameters of broiler. Asian J. Med. Biol. Res. June 2019, 5(2): 153-157

Toxic effect and oral acute LD50 study of Nerium oleander in male guineapigs

1970 ◽  
Vol 2 (2) ◽  
pp. 159-161 ◽  
Author(s):  
MGA Chowdhury ◽  
A Azizunnesa ◽  
MA Hossain ◽  
ML Rahman ◽  
Q Hasan
Keyword(s):  
Body Weight ◽  
Abdominal Pain ◽  
Oral Dose ◽  
Respiratory Distress ◽  
Toxic Effect ◽  
Single Oral Dose ◽  
Nerium Oleander ◽  
Male Adult ◽  
Experimental Animals ◽  
Group A

The toxic effect of Nerium oleander was studied in 36 male adult guineapigs during the period from July to December 1994. These 36 animals were divided into six equal groups (A to F), each consisting of six animals. Each animal of groups B to F was administered with a single oral dose of crude watery extract of sheath oleander @ 300, 450, 600, 750 and 900 mg / kg body weight, respectively whereas animals of group A served as control. Each of the experimental animals was carefully observed and the toxic signs recorded as nausea, anorexia, dullness, depression, restlessness, abdominal pain, salivation, reluctant to move, tremor, resting of chin on the ground, respiratory distress, paralysis of the limbs, recumbency, convulsion followed by death with characteristic groaning. It may be concluded that the lowest dose 300 mg / kg body weight is non lethal to the male guineapigs and the dose of 450, 600, 750 and 900 mg / kg body weight caused 17%, 50%, 83% and 100% mortality, respectively and the LD50 is 540 mg / kg body weight.Key words: Nerium oleander; toxic signs; oral acute LD50; guineapigsdoi: 10.3329/bjvm.v2i2.2562Bangl. J. Vet. Med. (2004). 2 (2): 159-161

Clinical Features of Epistaxis in Dogs: A Retrospective Study of 35 Cases (1999–2002)

2005 ◽  
Vol 41 (3) ◽  
pp. 179-184 ◽  
Author(s):  
Jennifer L. Strasser ◽  
Eleanor C. Hawkins
Keyword(s):  
Retrospective Study ◽  
Cell Volume ◽  
Clinical Features ◽  
Packed Cell Volume ◽  
Systemic Disease ◽  
Facial Deformity

Epistaxis was retrospectively evaluated in 35 dogs. Systemic disease was diagnosed in seven dogs and intranasal disease in 29. Nineteen dogs with intranasal disease had neoplasia. Dogs with neoplasia were older (mean 10.0 years) than dogs with nonneoplastic intranasal disease (mean 5.6 years). Signs persisting for &gt;1 month occurred more often in dogs with intranasal than systemic disease. Unilateral epistaxis did not distinguish intranasal from systemic disease. Only dogs with intranasal disease had facial deformity, decreased airflow, or regional sub-mandibular lymphadenopathy. Dogs with systemic disease had a lower packed cell volume (mean 31.8%) than dogs with intranasal disease (mean 42.7%).

scholarly journals Studi Histopatologi Limpa dan Bursa Fabricious Ayam Berpenyakit Tetelo (Newcastle Disease) pada Kasus Lapang (HISTOPATHOLOGY STUDIES ON SPLEEN AND BURSA OF FABRICIUS OF NEWCASTLE DISEASE CHICKHENS FROM FIELD CASE)

2018 ◽  
Vol 18 (4) ◽  
pp. 510
Author(s):  
Etriwati Etriwati ◽  
Ekowati Handharyani ◽  
Surachmi Setiyaningsih
Keyword(s):  
Newcastle Disease ◽  
Immunohistochemical Staining ◽  
Lymphoid Cells ◽  
Bursa Of Fabricius ◽  
Mantle Zone ◽  
Lymphoid Follicles ◽  
Degree Of Reaction ◽  
Avian Disease ◽  
Virus Distribution ◽  
Red Pulp

Newcastle Disease is a highly contagious and very virulent avian disease in all of poultry. The aims of this study were to determine the lesions and distribution of Newcastle disease virus on spleen and bursa of Fabricous of Newcastle disease chickhens from field cases. The sample used in this study were the spleen and bursa Fabricious organ of ten cases of suspect Newcastle disease. Haematoxylin and eosin staining examination was performed determine of cell morphology and distribution of the virus by immunohistochemical staining. The distribution of the lesions and the degree of reaction were determined by the category immunopositive mild, moderate and severe. Gross lesion on spleen, including swollen, fragile and necrotic multifocal. Histopathological lesions showed hyperemia/congestion and depletion of lymphoid cells. Immunopositive reaction found in lymphoid cells of red pulp and lymphoid cells in lymphoid follicles. Bursa of Fabricious showed atrophy and hyperemia/congestion with histopatological lesions hemorrhages, lymphoid cells in lymphoid follicles undergo necrosis and depletion of lymphoid follicles. Immunopositif reaction was observed in lymphoid cells in the mantle zone of lymphoid follicles. Newcastle disease from field cases cause damage to the spleen and bursa of Fabricius with degrees lesions and virus distribution in the organs are severe.

scholarly journals Subchronic Acephate Exposure Induces Immunotoxicity in White Leghorn Cockerels

2021 ◽  
Author(s):  
Syamantak Tripathi ◽  
Prem Govindappa ◽  
Megha Bedekar ◽  
Yash Sahni ◽  
B. Sarkhel ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Body Weight Gain ◽  
Animal Health ◽  
Acetylcholinesterase Activity ◽  
Bursa Of Fabricius ◽  
Delayed Type Hypersensitivity ◽  
White Leghorn ◽  
Groundnut Oil ◽  
Con A ◽  
Live Body Weight

Abstract We investigated the subchronic immunotoxicity of the phosphoramidothioate organophosphorous insecticide, acephate in white leghorn cockerels (WLH). The cockerels were divided into five groups; C1 (plain control), C2 (vehicle control), T1, T2, and T3 which received acephate suspended groundnut oil for 60 days at doses of 21.3, 28.4 and 42.6 mgkg−1respectively. The live body weight gain, absolute and relative weights of the spleen, thymus, and bursa of Fabricius, hemoglobin (Hb), total erythrocyte counts (TEC), packed cell volume (PCV) and lymphocytes were significantly decreased. However, monocytes, eosinophils, heterophils, and basophils were significantly increased. Total protein, albumin and albumin to globin ratio, the antibody response to RD-F and delayed-type hypersensitivity response to DNCB dye or PHA-P, erythrocyte and brain Acetylcholinesterase activity was also significantly reduced in T2 and T3. At 40 and 60 days of acephate exposure, nitrate and nitric oxide production by RD-F and mitogen Con A stimulated peripheral blood and splenic lymphocytes, as well as lymphocyte proliferation in response to antigen RD-F and mitogen Con A stimulation, were significantly decreased in groups T2 and T3. Furthermore, dose-dependent increases in the frequency of micronuclei formation, varying intensity serum protein bands with different protein fractions (14.85KDa), and splenic DNA laddering (180 bp) were observed in groups T2 and T3. Histopathologically, the spleen and bursa showed morphological changes and mild lymphocyte depletion. In conclusion, low-level acephate exposure may affect acetylcholinesterase, lymphocytes, and immune responses in cockerels. As a result, it should be considered when assessing immunotoxicity and the risk to human and animal health.

scholarly journals Impact of parity and gestational age of mothers with hypertension on birth weight, red blood cells and mode of delivery of their babies

1970 ◽  
Vol 19 (4) ◽  
pp. 3038-3044
Author(s):  
Helen Chioma Okoye ◽  
Chilota Chibuife Efobi ◽  
Josephat Maduabuchi Chinawa ◽  
Odutola Israel Odetunde ◽  
Awoere Tamunosiki Chinawa ◽  
...  
Keyword(s):  
Birth Weight ◽  
Red Blood Cells ◽  
Cell Volume ◽  
Gestational Age ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
Mode Of Delivery ◽  
Poor Correlation ◽  
Hypertensive Group ◽  
The Impact

Background: Maternal factors are determinants of birth outcome which includes birth weight, haematological indices and mode of delivery of their babies.Objectives: To determine the impact of parity and gestational age of hypertensive mothers on some neonatal variables.Methods: A hospital based cross-sectional study of measurement of neonatal variables (birth weight, red blood cells and mode of delivery) among hypertensive mothers and their controls was conducted over a period of six months. Data were analyzed using the Statistical Package for Social Sciences program (SPSS), version 20.Results: There were statistically significant differences in means between the neonates of the hypertensive group and non-hypertensive group for maternal age (t =1.61, p = 0.002), baby weight (t =2.87, p < 0.001), haemoglobin (Hb) (t =4.65, p = 0.010) and packed cell volume (PCV) (t =4.75, p = 0.009), but none for gravidity (t =1.95, p = 0.927)For all subjects, there was poor correlation between gestational age and variables; birth weight , haemoglobin (Hb), packed cell volume (PCV), nucleated red blood cell (nRBC) and parity. Likewise, parity poorly correlated with variables; age, birth weight, Hb, PCV, and nRBC. There was a statistically significant association between mode of delivery and hypertension (χ2 =53.082, p <0.001) but none with having a family history of hypertension (χ2 =1.13, p = 0.287).Conclusion: Parity and gestational age of mothers with hypertension have no impact on birth weight and red cells when compared with their non-hypertensive counterparts. However, mothers of babies delivered by elective and emergency caesarean section were about 2-3 times more likely to be hypertensive than those that delivered through spontaneous vertex delivery.Keywords: Hypertension; neonate; gestational age; parity.

scholarly journals The use of a standard operating procedure in the analysis of canine packed cell volume

2018 ◽  
pp. 457-458
Author(s):  
Craig R Breheny ◽  
Jorge Perez-Accino Salgado ◽  
Nicholas X Bommer ◽  
Ian Handel ◽  
Adam G Gow
Keyword(s):  
Cell Volume ◽  
Operating Procedure ◽  
Packed Cell Volume ◽  
Standard Operating Procedure ◽  
Standard Operating
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