scholarly journals Studies on effects of lactose on experimental Trypanosoma vivax infection in Zebu cattle. 2. Packed cell volume

2008 ◽  
Vol 75 (3) ◽  
Author(s):  
M.Y. Fatihu ◽  
S. Adamu ◽  
I.A. Umar ◽  
N.D.G. Ibrahim ◽  
L.O. Eduvie ◽  
...  
Keyword(s):  
Cell Volume ◽  
Normal Saline ◽  
Packed Cell Volume ◽  
Rate Of Change ◽  
Zebu Cattle ◽  
Trypanosoma Vivax ◽  
Rates Of Change ◽  
Group A ◽  
The Mean ◽  
Infusion Period

The ability of intravenously administered lactose in normal saline to prevent a decline in packed cell volume (PCV) during experimental trypanosomosis was studied in Zebu cattle. During the lactose infusion period, the PCV was stable up to Day 5 post-infection (p.i.) in a lactose-infused group, compared to that in an uninfused group in which the PCV dropped significantly (P < 0.05) as shown by the values of cumulative percentage change. Furthermore the mean rate of change in PCV was significantly (P < 0.05) higher in the uninfused group relative to the lactose-infused group during the same period. While the PCV fell markedly in the lactose-infused group a day after lactose infusion was stopped (Day 13 p.i.), subsequent PCV values were significantly (P < 0.05) higher compared to those in the uninfused group, up to the end of experiment on Day 17 p.i. However the mean rates of change in PCV did not vary significantly (P > 0.05) between the groups during the period in which lactose infusion was stopped. The mean levels of parasitaemic waves and parasitaemia were higher, more prolonged and more frequent in the lactose-infused group. It was inferred that the lactose was able to prevent an early onset of anaemia in the Trypanosoma vivax-infected Zebu cattle.

Studies on the habits of zebu cattle. III. Water consumption of Zebu cattle

1955 ◽  
Vol 46 (1) ◽  
pp. 123-129 ◽  
Author(s):  
D. H. L. Rollinson ◽  
K. W. Harker ◽  
J. I. Taylor
Keyword(s):  
Air Temperature ◽  
Water Consumption ◽  
Significant Positive Correlation ◽  
Negative Relationship ◽  
Zebu Cattle ◽  
Positive Correlation ◽  
Dry Air ◽  
Significant Negative Relationship ◽  
Group A ◽  
The Mean

In continuation of a series of studies on the habits of Zebu cattle, records for the quantities of water consumed every hour by ten cattle during twenty periods of 24 hr. observations have been analysed.A large variation in day-to-day consumption was evident, but water was taken on an average during 8 hr. of the day with only a small amount of water consumed during darkness. The mean consumption per animal per 24 hr. period was 9·04 kg. (1·9 gal.). The range was 5·1–12·7 kg. (1·1–2·8 gal.).The water consumption records were subdivided into those days on which water was drunk during a small number of hours and those days when water was drunk during many hours. Although the hourly consumption differed markedly the end result showed a similar intake.A significant negative relationship was found between water consumed and grazing counts, and for one group a significant positive correlation was found between water consumption and dry air temperature.

Mechanism of potassium uptake in neuropile glial cells in the central nervous system of the leech

1990 ◽  
Vol 63 (5) ◽  
pp. 1089-1097 ◽  
Author(s):  
W. A. Wuttke
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cell Volume ◽  
Normal Saline ◽  
Cell Swelling ◽  
Equilibrium Potential ◽  
K Uptake ◽  
The Central Nervous System ◽  
The Mean ◽  
K Concentration

1. Ion-selective double-barreled microelectrodes (ISME) were used to measure intracellular K+ (aKi), Na+ (aNai), and Cl- (aCli) activities of neuropile glial (NG) cells in the central nervous system of the medicinal leech Hirudo medicinalis. Ion fluxes were induced by an increase in extracellular K+ concentration [( K+]o) and analyzed to elucidate the ionic mechanism of the K+ uptake occurring under such conditions. 2. In addition, the K+ concentration of the extracellular space of the nerve cell body region (NCBR) and the neuropile (N) was measured with neutral carrier K(+)-ISME. In normal saline (4 mM K+), a concentration of 4.2 mM was measured in both extracellular spaces. No differences between the K+ concentration of the bathing fluid and the extracellular spaces were found at higher (i.e., 10 and 40 mM) K+ concentrations. 3. In normal saline, the mean membrane potential (Em) was -68 mV, and the mean aKi, aNai, and aCli were found to be 77, 10, and 7 mM, respectively. The corresponding equilibrium potentials were -81, 56, and -66 mV. The chloride equilibrium potential (ECl) was similar to Em, and it is concluded that chloride is passively distributed across the NG cell membrane. 4. When [K+]o was transiently increased 10-fold (i.e., to 40 mM), aKi and a Cli increased transiently by 22 and 25 mM, respectively, and the membrane depolarized to -28 mV, which was similar to both K+ equilibrium potential (EK) and ECl. The KCl uptake was accompanied by a transient decrease in aNai to 5 mM. 5. After incubation for at least 1 h in Na(+)-free saline, NG cells accumulated K+ in the absence of extracellular Na+ to levels similar to those observed in the presence of Na+. Therefore the uptake of K+ was not dependent on external--and probably also internal--Na+. 6. Changes in cell volume induced by the increase in [K+]o were estimated by loading NG cells with choline and monitoring its intracellular concentration with Corning-K(+)-ISME. In saline containing 40 mM K+, NG cell volume increased to approximately 150% of its volume in normal saline. 7. It is concluded that the mechanism of K+ uptake in NG cells is by passive KCl and water influx, which causes cell swelling.

Histopathological alterations of selenium toxicity induced in broiler (Birds)

2017 ◽  
Author(s):  
Avnish Kumar Gautam ◽  
Deepak Kumar ◽  
Manoj Kumar Sinha
Keyword(s):  
Drinking Water ◽  
Toxic Effect ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Sodium Selenite ◽  
Biochemical Parameter ◽  
Lymphoid Cells ◽  
Bursa Of Fabricius ◽  
White Leghorn ◽  
Group A

A study was conducted to determine the pathological toxic effect of selenium (Sodium selenite). A total number 120 one day old White Leghorn (WLH) healthy broiler birds were randomly divided into A, B and C groups. Each group contain 40 birds daily administration of sodium selenite @ 30ppm and @ 15 ppm in group A and B, respectively and group C was given plain drinking water daily for 42 days and kept as control. Macroscopically and microscopically revealed varying degrees of congestion and haemorrhages in lungs, liver, kidneys, heart and intestine in selenium treated birds. The bursa of Fabricius showed depletion of lymphoid cells between the interfollicular spaces. A significant (P less than 0.05) decrease in the haemoglobin and packed cell volume was noticed in both the selenium fed groups but the total erythrocyte count remain unchanged. Biochemical parameter revealed slight decline in their activity of serum ALT and AST and increased level of BUN and creatinine in group A and B as compared to group C, suggesting some degree of renal dysfunctioning.

scholarly journals Prevalence of Anaemia at Booking amongst Antenatal Clinic Attendees in Some Health Facilities in Ogoni, South-South Nigeria

2021 ◽  
pp. 176-182
Author(s):  
S. Eli ◽  
G. P. Tee Popnen ◽  
D. G. B. Kalio ◽  
N. C. T. Briggs ◽  
K. E. Okagua
Keyword(s):  
Developing Countries ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Antenatal Clinic ◽  
Health Care Facilities ◽  
The World ◽  
The Mean ◽  
Anaemia In Pregnancy ◽  
In Pregnancy

Background: Anaemia in pregnancy is an indirect cause of maternal mortality. Myriad of factors predispose pregnant women to anaemia in pregnancy in the developing countries of the world of which poverty, illiteracy and ignorance are central. The prevalence of anaemia in the developing countries of the world is between 32% and 62.2%. Objective: To determine the prevalence of anaemia amongst antenatal clinic attendees in Ogoni, South-South Nigeria and offer preventive measures. Material and Methods: This was a retrospective four months cross-sectional study of antenatal clinic attendees at booking in three (3) primary health care facilities and general hospitals Bori and Tera, in Ogoni, South-South Nigeria. Three hundred and eighty (380) pregnant women’s biodata including their packed cell volume were collected. These pieces of information were computed using a structured proforma. The data were coded and analysed using statistical package for social sciences (SPSS) software version 25. Results: The mean age of the study antenatal clinic attendees was 28 years. The mean gestational age at booking was 25weeks. The range for the packed cell volume was 25% to 36% and the mean packed cell volume at booking was 30%. Three hundred and eighteen (318) (84.0%) had anaemia (with packed cell volume less than 33%). Majority of patients 249 (65.6%) had mild anaemia There was none with severe anaemia. Majority of patients had secondary level of education represented by 275 (72.3%). Conclusion: The prevalence of anaemia amongst antenatal clinic attendees at Ogoni was 84.0%. The role of pre-pregnancy care amongst women desirous of pregnancy, familly planning and the role of education cannot be over emphasized in the prevention of anaemia in pregnancy.

scholarly journals Effect of Physical Exercise on some Hematological Parameters in Female Athletes in Bangladesh

1970 ◽  
Vol 52 (195) ◽  
pp. 892-896 ◽  
Author(s):  
Tanbira Alam ◽  
Lubna Shirin ◽  
SM Niazur Rahman ◽  
Tanjeda Alam ◽  
Nasrin Habib ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Serum Iron ◽  
Female Athletes ◽  
Ferritin Level ◽  
Hematological Parameters ◽  
Control Group ◽  
Group A ◽  
Group B

Introduction:  Long term physical training has been considered to adversely affect the performance of athletes especially the females. It may be due to the iron depletion caused by hemolysis or hemodilution results from plasma volume expansion. This study aims to assess the effect of heavy exercise on hemoglobin concentration and some other hematological parameters in female athletes. Methods: This cross sectional study was carried out in the Department of physiology, Dhaka Medical College, Dhaka, from July 2010-June 2011. A total number of 105 females (70 athletes and 35 non athletes) between 15-25 years of age were recruited. The 35 apparently healthy female non athletes were taken as control (Group-A) and 70 female athletes comprise the study group (Group-B). According to sporting category, Group-B was further subdivided as B1:Runners and B2:Cyclists. Hemoglobin percentages (Hb %), red blood cell (RBC) concentration, packed cell volume (PCV), serum iron, serum ferritin and total iron binding capacity (TIBC) were measured in fasting state after two months training. Statistical analysis was done by using SPSS/ win.15.0 for comparison between the groups, using student’s unpaired “t” test. Results: Mean Hb%, RBC, PCV, iron and ferritin level were significantly (P<0.001) lower in athletes than those of control group. Within the study groups, runner’s serum iron and ferritin level were lower than cyclists.  TIBC significantly (P<0.001) rose in Group-B2 in comparison to Group-A. Conclusions: The current study showed that intense physical exercise leads to early stages of Hb%, RBC, PCV, iron and ferritin depletion which might compromise the health and performance of athletes. Keywords: exercise; hemoglobin; packed cell volume; serum iron.  

scholarly journals ANTI-ANAEMIC POTENTIAL OF METHANOLIC LEAF EXTRACT OF MUCUNA PRURIENS ON PHENYLHYDRAZINE (PHZ) INDUCED ANAEMIC ALBINO WISTAR RATS

2020 ◽  
Vol 4 (3) ◽  
pp. 370-374
Author(s):  
Kasang Naman ◽  
Habibat Oseni ◽  
Emmanuel Enoh
Keyword(s):  
Vitamin B12 ◽  
Cell Volume ◽  
Normal Control ◽  
Normal Saline ◽  
Packed Cell Volume ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Lethal Dose ◽  
Mucuna Pruriens ◽  
Control Group

The antianaemic potential of methanolic leaf extracts of Mucuna pruriens was investigated using phenylhydrazine (PHZ) induced anaemic albino Wistar rats.  Fifteen rats used for the study were randomized into five experimental groups. To induced anaemia, the rats (except the normal control, Group E), received 60 mg/kg of the haemolytic agent Phenylhydrazine intraperitoneally (i.p) for two consecutive days. Anaemic Wistar rats in groups A and B received a daily oral dose of 500 and 250 mg/kg of the methanolic leaf extract of Mucuna pruriens. Nweze et al. (2016) had reported a median lethal dose greater than 5000 mg/kg for the methanol leaf extract of Mucuna pruriens. Groups C and D received Vitamin B12 (10 mg/kg) and normal saline (1 ml/kg), respectively. Normal control rats also received normal saline (1 ml/kg). Extract or normal saline was administered per os (p.o) while vitamin B12 was administered i.p. for a duration of 21 days. Packed cell volume (PCV) and haemoglobin concentration were determined weekly for three weeks. The result of the study indicated that both the methanolic leaf extract of Mucuna pruriens and Vitamin B12 significantly (p < 0.05) increased the packed cell volume and haemoglobin concentrations in treated rats compared to the negative control group of rats. This indicated that the methanolic leaf extract of Mucuna pruriens has anti-anaemic properties and could be utilized in the management of anaemia

scholarly journals Further observations on the packed cell volume and haemoglobin concentration in cattle naturally infected with Fasciola gigantica

1970 ◽  
Vol 2 (2) ◽  
pp. 125-127
Author(s):  
MMR Howlader ◽  
S Begum ◽  
KhN Islam ◽  
MA Hai ◽  
MG Hossain
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Age Groups ◽  
Haemoglobin Concentration ◽  
Fasciola Gigantica ◽  
Zebu Cattle ◽  
Infected Cattle ◽  
Infected Control ◽  
Hb Concentration ◽  
Effect Of Age

The changes on packed cell volume (PCV) and haemoglobin (Hb) in zebu cattle infected with Fasciola gigantica were studied in two age and two season groups during the period from November 1999 to June 2000. Eighty-eight selected cattle were divided into two age groups, 2.5 to 5.5 years (n = 44; 22 infected & 22 uninfected control) and 6.0 to 9.0 years (n = 44; 22 + 22 cattle), and similarly two seasons, winter (n = 22 + 22) and summer (n = 22 + 22) were used for this study. Blood samples were collected in glass vials contained EDTA anticoagulant for each age group of all animals in summer and winter seasons. PCV was determined using microhematocrit and Hb concentration by cyanomethemoglobin methods. The PCV of F. gigantica infected cattle were significantly (p < 0.01) lower than the non-infected control animals. The average PCV obtained were 24.43% and 32.33% for F. gigantica infected and non-infected cattle, respectively. The Hb concentrations of infected cattle were significantly (p < 0.01) lower than the non-infected control animals. The average Hb values found were 7.59 and 10.13 g% for F. gigantica infected and noninfected cattle, respectively. There was no significant effect of age and season on the values of PCV and Hb concentrations in infected and non-infected cattle.Key words: Zebu cattle; Fasciola gigantica; packed cell volume; haemoglobin concentrationdoi: 10.3329/bjvm.v2i2.2553Bangl. J. Vet. Med. (2004). 2 (2): 125-127

scholarly journals Control of sheep gastrointestinal nematodes using the combination of Duddingtonia flagrans and Levamisole Hydrochloride 5%

2018 ◽  
Vol 27 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Vinícius Longo Ribeiro Vilela ◽  
Thais Ferreira Feitosa ◽  
Fabio Ribeiro Braga ◽  
Vanessa Diniz Vieira ◽  
Samuel Cavalcante de Lucena ◽  
...  
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Gastrointestinal Nematodes ◽  
Northeastern Brazil ◽  
Duddingtonia Flagrans ◽  
Group 3 ◽  
The Mean ◽  
Group 2 ◽  
Group 1

Abstract The objective was to evaluate the action of D. flagrans pellets in association with Levamisole Hydrochloride 5% for controlling sheep gastrointestinal nematodes in the northeastern Brazil. Three groups of six sheep each were formed: group 1 received 3 g of the pellets (0.6 g of D. flagrans mycelium) for each 10 kg b.w., twice a week for six months, and deworming with Levamisole Hydrochloride 5% when EPG ≥ 1500; group 2 received a dosage of Levamisole Hydrochloride 5% when EPG ≥ 1500; and group 3 received 3 g of pellets without fungi for each 10 kg b.w., twice a week for six months. EPG counts, larval cultures, packed cell volume (PCV) and weighing were performed every 15 days; monthly, samples of grass from each paddock were collected. The mean EPG of the groups began to statistically differ from day 30 (p < 0.05). Group 1 required less deworming with Levamisole Hydrochloride 5% and showed superiority of PCV values throughout the experiment (p < 0.05). There was a significant reduction (p < 0.05) in L3 recovery in the group 1 paddock from day 30 onwards. The use of D. flagrans pellets in association with Levamisole Hydrochloride 5% was effective for controlling gastrointestinal nematodes.

scholarly journals The comparison of the efficacy of intra-peritoneal instillation along with local infiltration with bupivacaine versus placebo for post-operative analgesia after laparoscopic tubal ligation

2019 ◽  
Vol 8 (8) ◽  
pp. 3268
Author(s):  
Aniket S. Kakade ◽  
Naval Dudam ◽  
Tushar M. Panchanadikar
Keyword(s):  
Pain Relief ◽  
Normal Saline ◽  
Tubal Ligation ◽  
Medical Institute ◽  
Care Hospital ◽  
Local Infiltration ◽  
Post Operative Pain ◽  
Group A ◽  
The Mean ◽  
Group B

Background: Intra-peritoneal instillation of local anesthetics has been shown to minimize post-operative pain after laparoscopic surgeries. The present study was undertaken with the following aim to compare the efficacy of intra-peritoneal instillation and local infiltration with bupivacaine versus placebo for post-operative analgesia after laparoscopic tubal ligation. To correlate the advantages and side effects of the drugs during the procedure. To establish a protocol for the use of bupivacaine instillation and infiltration for post-operative analgesia after laparoscopic tubal ligation.Methods: A prospective study was carried out in a tertiary care hospital attached to a teaching medical institute. Institutional ethics committee approval was obtained for this randomized control study (double blind) including 50 women who will be divided in two groups. Group A will be administered intra-peritoneal bupivacaine along with local infiltration. Group B will be administered normal saline. Post-operative pain will be monitored by a blind observer and rescue analgesia will be administered at visual analogue score of 3.Results: Both the groups were comparable with respect to age and BMI. The mean duration of analgesia in Group A with bupivacaine was 315.60±79.9 min (Median 300 min).  The mean duration of analgesia in Group B with normal saline was 138.20±46.97 min (Median 150 min, p value <0.001).Conclusions: The study depicts a significant pain relief after use of bupivacaine. Bupivacaine instillation in the peritoneal cavity along with local infiltration of the drug gives prolongs pain relief as compared to normal saline.

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