Molecular identification studies  on Oestrus ovis L. (Diptera:Oestridae) larvae infested sheep in jazan region , Saudi Arabia

2017 ◽  
Author(s):  
Bosly . A. Hanan
Keyword(s):  
Saudi Arabia ◽  
Molecular Identification ◽  
Gene Sequence ◽  
Morphological Identification ◽  
Oestrus Ovis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Partial Fragment ◽  
Mitochondrial Cytochrome Oxidase ◽  
Mtcoi Gene

Oestrus ovis L. (O. ovis) (Diptera: Oestridae) (Sheep Bot Fly) is ubiquitous in distribution. Myiasis causing larvae collected from Abu-Arish area (Eastern Jazan), Saudi Arabia were confirmed as O. ovis based on morphological traits. Polymerase chain reaction (PCR) studies targeting amplification of partial fragment (606 bp) of mitochondrial cytochrome oxidase subunitI (mtCOI) gene further confirmed the species. Phylogenetic analysis based on the partial (mtCOI) gene sequence revealed that the accession KU921431 showed 97% similarity based on nucleotide pairs of O.ovis accessions, retrieved from the GenBank. Thus, a method of molecular identification of O.ovis larvae was established as a credible substitution to morphological identification in Jazan region, Saudi Arabia.

scholarly journals Prevalence Rate and Molecular Characteristics of Oestrus ovis L. (Diptera, Oestridae) in Sheep and Goats from Riyadh, Saudi Arabia

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 689
Author(s):  
Dina M. Metwally ◽  
Shurug A. Albasyouni ◽  
Ibrahim A.H. Barakat ◽  
Isra M. Al-Turaiki ◽  
Amal M. Almuhanna ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Gene Sequence ◽  
Morphological Characteristics ◽  
Pcr Amplification ◽  
Infestation Rate ◽  
Molecular Characteristics ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Partial Fragment ◽  
Mtcoi Gene

Heads of sheep (n = 600) and goats (n = 800) slaughtered at Al-Aziziah Abattoir in Riyadh, Saudi Arabia, were inspected for the presence of O. ovis larvae (L). Heads were split along the longitudinal axes, and larvae (L1, L2, and L3) were gathered. The infestation rate was significantly higher in goats (44.5%; 356/800) than that in sheep (22.3%; 134/600). Out of the 151 collected larvae from sheep, 0% were L1, 1.3% were L2, and 98.7% were L3. Out of the total of 468 larvae from goats, 0% were L1, 1.2% were L2, and 98.8% were L3. The infestation rate was significantly higher in males than that in females. Myiasis-causing larvae collected from Riyadh, Saudi Arabia, were authenticated as O. ovis, according to morphological characteristics. Polymerase chain reaction (PCR) amplification of a partial fragment (600 bp) of the mitochondrial cytochrome c oxidase subunit I (mtCOI) gene further confirmed the species. Phylogenetic analysis based on the partial mtCOI gene sequence demonstrated that 23 unique sequences showed high similarity based on nucleotide pairs of O. ovis accessions retrieved from GenBank.

scholarly journals A simple PCR-based method for the rapid and accurate identification of spider mites (Tetranychidae) on cassava

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Tatiana M. Ovalle ◽  
Aymer Andrés Vásquez-Ordóñez ◽  
Jenyfer Jimenez ◽  
Soroush Parsa ◽  
Wilmer J. Cuellar ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Crop Protection ◽  
Restriction Enzymes ◽  
Mite Species ◽  
Morphological Identification ◽  
Chain Reaction ◽  
Accurate Identification ◽  
Polymerase Chain ◽  
Cassava Crop ◽  
Mitochondrial Cytochrome Oxidase

Abstract The morphological identification of mites entails great challenges. Characteristics such as dorsal setae and aedeagus are widely used, but they show variations between populations, and the technique is time consuming and demands specialized taxonomic expertise that is difficult to access. A successful alternative has been to exploit a region of the mitochondrial cytochrome oxidase I (COI) gene to classify specimens to the species level. We analyzed the COI sequences of four mite species associated with cassava and classified them definitively by detailed morphological examinations. We then developed an identification kit based on the restriction fragment length polymorphism–polymerase chain reaction of subunit I of the COI gene focused on the three restriction enzymes AseI, MboII, and ApoI. This set of enzymes permitted the simple, accurate identification of Mononychellus caribbeanae, M. tanajoa, M. mcgregori, and Tetranychus urticae, rapidly and with few resources. This kit could be a vital tool for the surveillance and monitoring of mite pests in cassava crop protection programs in Africa, Asia, and Latin America.

scholarly journals Culex (Diptera: Culicidae) Mosquitoes in Jazan Region, Saudi Arabia, and Their Molecular Identification

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Elsiddig Noureldin ◽  
Ommer Dafalla ◽  
Abdualziz Hakami ◽  
Mohammed Jubran ◽  
Ahmed Alzhrani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Saudi Arabia ◽  
Molecular Identification ◽  
Mosquito Species ◽  
Polymerase Chain Reaction Technique ◽  
Identification System ◽  
Culex Mosquito ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
First Time

Morphological characteristics have been the gold standard method to identify mosquito species. However, morphological identification has many limitations including lack of expertise and damaging of external characters due to improper specimen handling. Therefore, we used the polymerase chain reaction technique (PCR) as an integrated tool to identify Culex mosquito species to establish a more precise and reliable identification system related to their spatial distribution in Jazan region. We identified Culex mosquito species and subspecies using taxonomic keys, and then we used the polymerase chain reaction technique (PCR) as an integrated tool to confirm and refine the list of Culex mosquito species in the region. Phylogenetic trees were constructed for the identified species, and their distinctive clustering was compared with their reference’s species in the GenBank. We identified 7026 adult Culex mosquitoes belonging to 4 species. Culex tritaeniorhynchus was the predominant species (45%), followed by Cx. quinquefasciatus (32%), then Culex sitiens (20%), and Cx. pipiens (3%). The most infested areas by Culex in the region were Gizan and Sabya. The PCR achieved 100% success in identifying the four Culex mosquito species. We also report the molecular identification of Cx. quinquefasciatus and Cx. pipiens species for the first time in Jazan region while the molecular identification of Cx. tritaeniorhynchus and Cx. sitiens was reported for the first time in Jazan region and the whole Saudi Arabia. This study utilized for the first time PCR to identify Culex mosquito species in Jazan region. The PCR is a complementary and integrated taxonomy-based identification tool for mosquito species. This integration has the capacity to promote and enhance vector surveillance and control programs, as well as defining the genetic diversity of species in the region.

scholarly journals Pectobacterium spp. Associated with Bacterial Stem Rot Syndrome of Potato in Canada

2012 ◽  
Vol 102 (10) ◽  
pp. 937-947 ◽  
Author(s):  
S. H. De Boer ◽  
X. Li ◽  
L. J. Ward
Keyword(s):  
Gene Sequence ◽  
Blackleg Disease ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Pectobacterium Atrosepticum ◽  
Physiological Characterization ◽  
Specific Polymerase Chain Reaction ◽  
Sequence Identification ◽  
Carotovorum Subsp ◽  
Polymerase Chain

Pectobacterium atrosepticum, P. carotovorum subsp. brasiliensis, P. carotovorum subsp. carotovorum, and P. wasabiae were detected in potato stems with blackleg symptoms using species- and subspecies-specific polymerase chain reaction (PCR). The tests included a new assay for P. wasabiae based on the phytase gene sequence. Identification of isolates from diseased stems by biochemical or physiological characterization, PCR, and multi-locus sequence typing (MLST) largely confirmed the PCR detection of Pectobacterium spp. in stem samples. P. atrosepticum was most commonly present but was the sole Pectobacterium sp. detected in only 52% of the diseased stems. P. wasabiae was most frequently present in combination with P. atrosepticum and was the sole Pectobacterium sp. detected in 13% of diseased stems. Pathogenicity of P. wasabiae on potato and its capacity to cause blackleg disease were demonstrated by stem inoculation and its isolation as the sole Pectobacterium sp. from field-grown diseased plants produced from inoculated seed tubers. Incidence of P. carotovorum subsp. brasiliensis was low in diseased stems, and the ability of Canadian strains to cause blackleg in plants grown from inoculated tubers was not confirmed. Canadian isolates of P. carotovorum subsp. brasiliensis differed from Brazilian isolates in diagnostic biochemical tests but conformed to the subspecies in PCR specificity and typing by MLST.

Gene sequences of the pcpB gene of pentachlorophenol-degrading Sphingomonas chlorophenolica found in nondegrading bacteria

1998 ◽  
Vol 44 (7) ◽  
pp. 667-675 ◽  
Author(s):  
Vandana M Saboo ◽  
Michael A Gealt
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Sequence ◽  
Acid Methyl Ester ◽  
Solid Support ◽  
Contaminated Site ◽  
Chain Reaction ◽  
Hybridization Data ◽  
Acid Methyl ◽  
Sphingomonas Chlorophenolica ◽  
Polymerase Chain

Bacteria isolated from a pentachlorophenol (PCP) contaminated site grew in the presence of 50 µg PCP/mL but were not able to degrade it in either liquid medium or the presence of 1% sterile potting soil as a solid support. Probes developed using the gene sequence of PCP-4-monooxygenase (pcpB) from Sphingomonas chlorophenolica sp.nov hybridized to two separate isolates. Identification based on fatty acid methyl ester profiles (Sherlock™), substrate utilization (BIOLOG™), and 16S rRNA showed that the two strains were different from each other and from Sphingomonas chlorophenolica. Sequences from these isolates, amplified by polymerase chain reaction, confirmed the homology with pcpB. The presence of pcpB sequences in these nondegraders indicated that growth and hybridization data alone were insufficient for predicting degradation capability. Key words: pentachlorophenol, Sphingomonas chlorophenolica, pcpB gene, pentachlorophenol-4-monooxygenase.

scholarly journals Association of Alcaligenes Faecalis Strain in Juvenile Earthworms, from Cocoons of Eudrilus Eugeniae

2019 ◽  
Vol 9 (2S2) ◽  
pp. 688-692
Keyword(s):  
16S Rrna ◽  
Gene Sequence ◽  
Agar Medium ◽  
Alcaligenes Faecalis ◽  
Rrna Gene ◽  
Eudrilus Eugeniae ◽  
Zone Of Inhibition ◽  
Chain Reaction ◽  
Nutrient Agar Medium ◽  
Polymerase Chain

Cocoons of earthworm Eudrilus eugeniae were collected from vermiculture bed and found that it had antibacterial activity. The size of zone of inhibition was directly proportional to the size of cocoons examined. Along with nutritious fluid and embryos, culturable bacterial community was found inside the cocoons. Bacterial colonies were isolated from the trails of newly hatched, juvenile worms in the nutrient agar medium and examined. Gram negative, rod shaped bacterium was found to be abundant in the trails of juvenile earthworms. Polymerase chain reaction was performed from this bacterium to amplify the gene of 16S rRNA and analyzed. Subsequent bi-directional DNA sequencing revealed that this abundant bacterium is highly related to 16S rRNA gene sequence of a strain, Alcaligenes faecalis. Based on available literature, we hypothesize that this bacterium could be symbiotically associated with cocoons of earthworms.

scholarly journals Application of advanced molecular techniques to detect vector borne pathogens from stray dogs and cats in two different climatic zones of Saudi Arabia

2020 ◽  
Author(s):  
Abdullah D Alanazi ◽  
Jan Šlapeta ◽  
Abulaziz Alouffi ◽  
Nichola Calvani ◽  
Mohamed Alyousif ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Middle Eastern ◽  
High Elevation ◽  
Molecular Techniques ◽  
Limited Information ◽  
Chain Reaction ◽  
Climatic Zones ◽  
Vector Borne Diseases ◽  
Vector Borne ◽  
Polymerase Chain

Abstract Background: Vector-borne diseases have been increasing worldwide and reported in many animals including dogs and cats. Limited or no data are currently available regarding canine and feline vector-borne diseases in Saudi Arabia and limited information is available from other Middle Eastern countries. The aim of this study was to compare vector-borne disease prevalence between two bio-climatically distinct regions of Saudi Arabia, Riyadh province that is arid positioned at low elevation and Asir province that is humid at high elevation. Methods: Blood samples from 74d ogs from Riyadh province and 70 dogs and 44 cats from Asirprovince were collected and examined for the presence of genomic DNA of Babesias pp, Anaplasma spp., Ehrlichias pp., Bartonella spp., Mycoplasma spp., and Hepatozoon spp. by polymerase chain reaction (PCR), Multiplex-tandem PCR (MT-PCR) and Sanger sequencing.Results: Seventy four dogs were tested from Riyadh province and found be negative of any pathogen. Of the 70 dogs examined from Asir province 45(64.3%) were positive. Specifically, 40 (57.1%) dogs were positive for A.platys, 20 (28.5%) for B.vogeli, 11(15.7%) for My.Haemocanis, two (2.85%) for Candidatus Mycoplasma haematoparvum and one (1.4%) for Br.henselae. Fourteen out of 44 cats (31.8%) were positive for one of the detected vector-borne pathogens. Six cats (13.6%) were positive for Candidatus Mycoplasma haemominutum and My.haemofelis, respectively, four cats (9.2%) were positive for Br.Henselae, two (4.54%) for Candidatus Mycoplasma haematoparvum and one (2.27%) for A. platys. Conclusions: The results of this study report the occurrence of A. platys, B. vogeli, Br. henselae, and My. haemocanis in dogs and of A. platys, Br. henselae, My.haemofelis and Candidatus Mycoplasma haemominutum in cats from Asir province Further molecular investigations are strongly recommended in order to reduce the risk of dogs and cats acquiring vector-borne diseases in Saudi Arabia.

scholarly journals Two new species of planthoppers from India (Hemiptera: Auchenorrhyncha: Delphacidae) in the genera Parasogata and Eoeurysa

2020 ◽  
Vol 724 ◽  
pp. 93-108
Author(s):  
N Ramya ◽  
Charles Bartlett ◽  
Naresh M. Meshram
Keyword(s):  
New Species ◽  
Molecular Identification ◽  
Gene Sequence ◽  
Himachal Pradesh ◽  
Key To Species ◽  
Northeastern India ◽  
Two New Species ◽  
Mtcoi Gene

The genus Parasogata Zhou, Yang & Chen, 2018 is here reported from India represented by the new species Parasogata sexpartita sp. nov. collected in a recent exploration and survey of delphacids from Nagaland in northeastern India. A second species of Eoeurysa Muir, 1913 from India, the new species Eoeurysa sagittaria sp. nov., was found in Rampur, Una, Himachal Pradesh. Both new species are described with illustrations, and a molecular identification is given with the mtCOI gene sequence. A modified key to species of the genera is also provided.

scholarly journals First Molecular Identification of Sunfish in North Bali Water

2019 ◽  
Vol 3 (1) ◽  
pp. 12
Author(s):  
I Made Oka Riawan ◽  
Gede Iwan Setiabudi ◽  
I Made Merdana ◽  
I Putu Mangku Mariasa ◽  
Kadek Teguh Wirasastra
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Identification ◽  
Polymerase Chain Reaction Method ◽  
Accession Number ◽  
Chain Reaction ◽  
Reaction Method ◽  
D Loop ◽  
Reverse Primer ◽  
Polymerase Chain ◽  
Full Body

Stranded Sunfish in North Bali with full body we collect to do molecular identification. Samples were amplified at the d-loop locus (control region) using the PCR (Polymerase Chain Reaction) method. Primers used in PCR are H16498 as primary front (forward) and L15812 as reverse primer. Similarity value of 95% after alignment with Mola ramsayi (accession number accession AY940824) on GenBank, and the gaps of the nucleotide just 1%. The stranded sunfish identified using partial sequence mtDNA is the same species as the species Mola ramsayi.

scholarly journals Genetic diversity of Ehrlichia ruminantium strains in Cameroon

2014 ◽  
Vol 81 (1) ◽  
Author(s):  
Seraphine N. Esemu ◽  
Roland N. Ndip ◽  
Lucy M. Ndip
Keyword(s):  
Genetic Diversity ◽  
Deoxyribonucleic Acid ◽  
Amino Acid Level ◽  
Gene Products ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Ehrlichia Ruminantium ◽  
Polymerase Chain ◽  
Study Sites ◽  
Partial Fragment

In order to investigate the extent of genetic diversity among Ehrlichia ruminantium strains in Cameroon, a partial fragment (800 bp) of the E. ruminantium map1 gene was amplified by nested polymerase chain reaction in 121 of 156 E. ruminantium pCS20-positive DNA samples extracted from ticks and cattle collected from two ranches. Deoxyribonucleic acid sequencing of the map1 gene products indicated the presence of at least 21 genotypes at the nucleotide level and 16 genotypes at the amino acid level circulating within the study sites. Some of the genotypes were identical to Antigua (U50830), Blaaukrans (AF368000) or UmBanein (U50835), whilst the others were new genotypes. Twenty-four representative sequences were deposited in GenBank and given accession numbers JX477663 – JX477674 (for sequences of tick origin) and JX486788 – JX486799 (for sequences of cattle origin). Knowledge of E. ruminantium strain diversity could be important in understanding the epidemiology of heartwater

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