scholarly journals Detection of Flowering Ability on Several Bulbs Shallot Sources by using Hd3a and Endogenous GA3 Analysis

Author(s):  
E. Triharyanto ◽  
D. Purnomo ◽  
A. Yunus ◽  
Samanhudi .

Background: Problems shallot cultivation in tropical regions such as Indonesia, is low productivity. Planting material is one of the causes of low productivity. The planting material used is a bulb that has been used continuously. It is makes planting material susceptible to infectious diseases and viruses. Efforts can be made to reducing the attack of virus infections in the planting material of the bulbs is by using seeds. However, seed production in Indonesia is still low due to the difficulty of flowering and low viability of seeds. The aim of this research was to detect flowering ability from the source of bulbs flowering and non-flowering clumps using Hd3a primers and endogenous GA3, also the effect of age of bulbs to flowering of shallot. Methods: The study used a complete randomized block design. The treatment consisted of two factors with three replications. The first factor was source of bulbs flowering and non-flowering clumps. The second factor was age bulbs which consists of three levels, namely the 60, 80 and 90 days age bulbs. The contents of gibberellins was analyzed using HPLC analysis. Molecular analysis to determine the potential of flowering genes used SSR primer (Hd3a). Result: Hd3a primer can be used as a marker of flowering shallot. Source of bulbs flowering and non-flowering clumps have the same flowering potential gene. The percentage of flowering in flowering clumps was higher than non-flowering clumps and correlates with endogenous gibberellins content. The age of bulbs indicates that older bulbs gave a higher percentage of flowering.

Author(s):  
E. Triharyanto ◽  
B. Pujiasmanto ◽  
G.C. Handoyo

Background: Shallots are one of the horticultural commodities with low production growth value in Indonesia. Shallots production in Indonesia is limited by the quality of planting material and the climate, especially temperature. The flowering of shallots is a significant obstacle to the regulation of tuber development and bulbils. This study aimed to examine the effect of treatment on the initiation of flowering and the formation of bulbils. Methods: The method used was a complete randomized block design with six treatments: without treatment, vernalization at 5oC, Immersion GA3 100 ppm, Immersion IAA 100 ppm, Immersion NAA 100 ppm, injury to the flower stalk and three replications. Result: The treatment of flowering initiation and the formation of bulbils had a significant effect on the flowering day, the number of flower stalks, the percentage of flowering plants, number weight of bulbils and weight of bulbils. GA3 and vernalization can speed up the appearance of flowers and increase the percentage of flowering plants. GA3 and the opening of the flower stalks can encourage the formation of bulbils with a higher weight and diameter of the tubers. The flowering day had a negative correlation with the number of bulbils formed. Bulbils formation requires a higher content of endogenous GA3. GA3 can initiate the flowering and formation of shallot bulbils.


Agrivet ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 48
Author(s):  
Van Basten ◽  
Lagiman Lagiman ◽  
Suwardi Suwardi

Pepper (Piper nigrum L.) is one of the spice plants that have high export value and propagated by cuttings. Use of internodes number and growth regulator were needed as a way to provide a good of planting material through cuttings. This research aimed to determine the best interaction between internodes number and concentration of Rootone-F growth regulator on growth of pepper (Piper nigrum L.) by cuttings. This research was carried out in experimental garden of Agriculture Universitas Pembangunan Nasional “Veteran” Yogyakarta, Ngemplak, Sleman, Yogyakarta, since February – May 2019. This research used a Randomized Block Design (RBD) with two factors. The first factor was internodes number which were one, two and three internodes. The second factor was concentration of Rootone-F which were 50 ppm, 100 ppm, and 150 ppm. So there were 3 x 3 = 9 treatments combination. Cuttings wit seven intenodes without Rootone-F were used as control. The result showed there were no interaction from two treatments in each parameter. There were no significant difference between control and treatment of each parameter. Internodes number has significantly affected in paramater percentage of life, day of emergence shoot, shoot length, number of leaves, number of root, root volume and shoot dry weight with the best result were cutting with 3 internodes. Concentration of Rootone-F has significantly affected in parameter percentage of life and day of emergence shoot, concentration 100 ppm and 150 ppm better than 50 ppm.Keyword: Pepper plant, cuttings, internodes, Rootone-F.


2018 ◽  
Vol 9 (2) ◽  
pp. 153-163
Author(s):  
Wiwik Ambarsari ◽  
Henly Yuliana

The study aimed to determine the effect of the combination of city waste compost and cattle manure on the growth and yield of pakcoy plants (Brassica rapa, L. Chinensis) in Aluvial, Indramayu Regency. The study was conducted at the Experimental Garden of the Faculty of Agriculture, Wiralodra University, Indramayu Regency from March to August 2018. This study used Factorial Pattern Randomized Block Design with two factors. The first factor is city waste compost and the second factor is cattle manure with 4 levels: 0%, 3.0%, 6.0%, and 9.0%, repeated twice. The results showed that there was an independent influence between city waste compost and cattle manure on plant height and number of leaves. The best combination to increase the feasibility weight of pakcoy consumption is by giving city waste compost 9.0% (S4) and cattle manure 6.0% (K3).


2020 ◽  
Vol 27 (1) ◽  
pp. 56-60
Author(s):  
Reena Joshi ◽  
Salil Tewari ◽  
Rajesh Kaushal

The experiment was conducted at Agroforestry Research Centre, of G. B. Pant University of Agriculture and Technology, Pantnagar to assess the effect of age (one and two year old), growing condition (intermittent misting and no misting) and different seasons (summer, monsoon, autumn and spring) on rooting and shooting of culm cuttings of Dendrocalamus asper. The experiment was laid out in factorial randomized block design with three replications. The findings indicated that in D. asper, one year old culm cuttings in the intermittent misting condition showed good results. The findings also indicated that shooting and rooting per cent of culm cuttings planted in different season showed response as Spring (March)> Summer (June)> Monsoon (August)> Autumn (October).


2021 ◽  
pp. 55-66
Author(s):  
Mulyati Mulyati ◽  
Baharuddin AB ◽  
R Sri Tejo Wulan

The use of inorganic fertilizers has an important role in increasing plant growth and production. But it also causes various problems such as decreasing soil organic matter, soil acidity, soil degradation, nutrient imbalance and increasing environmental pollution. Therefore, it needs to be balanced with the provision of organic fertilizers. A field experiment was conducted to study the effect of application of various doses of inorganic and organic fertilizers on N, P, K nutrient uptake and maize plant growth. The experimental design used was a randomized block design arranged in a factorial manner consisting of two factors. The first factor is the dose of inorganic fertilizer (A): 0, 150 and 300 kg ha-1 phonska and the second factor is organic fertilizer plus (P): 0, 10, 20 tons ha-1. The two factors were combined and each treatment combination was repeated three times. The data obtained were analyzed by analysis of variance at the 5% level of significance. The results showed that there was no interaction between the application of inorganic and organic fertilizers on the growth and nutrient uptake of N, P and K. However, the application of inorganic and organic fertilizers had a significant effect on all tested agronomic traits except for plant height. Nutrient uptake of N, P and K also increased significantly due to the application of inorganic and organic fertilizers. The balance of inorganic and organic fertilizer application increases the availability of nutrients as well as the uptake of N, P, and K nutrients by maize plants.


Author(s):  
Totong Siwanto ◽  
Sugiyanta , ◽  
Maya Melati

ABSTRACT<br />Application of organic fertilizer into the soil is designated as a source of macro, micro nutrients and organic acids; it also acts as soil ameliorant that improve physical, chemical and biological properties of soil. Application of high rates organic fertilizer has a constraint namely the availability and ease of application. Therefore, it is necessary to learn the use of organic fertilizer with low rates to improve the efficiency of chemical fertilizers. This research was aimed to study the effect of organic and inorganic fertilizers on growth and yield of paddy rice, as well as the efficiency of N, P, K inorganic fertilizer. Field experiment was conducted at Sawah Baru Research Station, University Farm IPB, Bogor, West Java from September to December 2013. The experiment used Randomized Block Design consisted of two factors: rates of organic fertilizer (0, 250, 500, 750, and 1,000 kg ha-1), and rates of inorganic fertilizer (0, 100, 200, 300, and 400 kg ha-1). The results suggested that the application of 1,000 kg organic fertilizer ha-1 + 400 kg inorganic fertilizer ha-1 did not show significant interaction. Application of organic fertilizer alone up to 1,000 kg ha-1 resulted in low growth and yield. Increasing rates of  application up to 400 kg inorganic fertilizer ha-1 increased the growth and yield of paddy rice. The highest N efficiency was 89.19% at a rate of 500 kg organic fertilizer ha-1 + 200 kg inorganic fertilizer ha-1, whereas the highest efficiency of P and K, respectively were 69.55% and 92.52% at a rate of 750 kg organic fertilizer ha-1 + 300 kg inorganic fertilizer ha-1.<br />Keywords: NPK, organic fertilizer, paddy rice


2021 ◽  
Vol 4 (2) ◽  
pp. 122-129
Author(s):  
Enda Sabda Gentri Sembiring ◽  
Julaili Irni ◽  
Rama Riana Sitinjak ◽  
Bayu Pratomo

Introduction: This study aims to determine the growth response of Mucuna bracteata cuttings to the concentration and duration of soaking shallot extract. Materials and Methods: This study used a factorial Randomized Block Design (RBD) with two factors, namely: shallot extract concentration (P) consists of 4 levels, include P0= untreated, P1= 10 ml, P2= 20 ml, P3= 30 ml, meanwhile the soaking time (Q) consists of 4 levels, such as Q0= momentarily dipping, Q1= 15 minutes, Q2= 30 minutes, Q3= 45 minutes. The data was processed by ANOVA and if significant, then further tested with Duncan Multiple Range Test (DMRT) at the rate of 5%. Results: The concentration of shallot extract, soaking time and their interaction had a very significant effect on the survival percentage of Mucuna bracteata D.C. cuttings. The highest survival percentage of cuttings of Mucuna bracteata on the effect of shallot extract concentration and soaking time was obtained in the P0Q0, P0Q1, and P3Q2 treatments after the lid was opened at 4 weeks after planting. In the number of leaves also had significant effect in the interaction of two factors


2021 ◽  
pp. 47-56
Author(s):  
Oki Puspita Wardani ◽  
Priyadi Priyadi ◽  
Yatmin Yatmin

This study was conducted on Pujodadi Village, Trimurjo, Central Lampung from September to December 2020. The study used a factorial randomized block design with two factors and three replications. The first factor is concentration of plant growth substances which consists of 5 levels; Z0 (0 ml.lt-1), Z1 (3.5 ml.lt-1), Z2 (7 ml.lt-1), Z3 (10.5 ml.lt-1) and Z4 (14 ml.lt-1). The second factor is the origin of the seed consists of three parts; P1 (top), P2 (middle), and P3 (bottom). The research objective was to study the effect of plant growth substances concentration and part of seed origin in sugarcane seedlings. The results showed that the plant growth substances concentration significantly increased the percentage of growth and the time 50% of the seeds sprouted. The seed origin of the top part showed the best growth compared to the middle and bottom. There was no interaction between plant growth substances concentration and seed origin on sugarcane seed growth.


2018 ◽  
Vol 45 (3) ◽  
pp. 249
Author(s):  
Solikin ,

Dioscorea is potentially used as staple food to support food security. The research was aimed to determine the effect of stake length and time of  stem twining  on  the growth of Dioscorea sansibarensis Pax. The experiment was conducted  in  Purwodadi Botanic Garden from December 2014 until May 2015 using split plot randomized block design consisted of two factors, i.e. the stake length and time of stem twining. The stake length was the main plot consisted of 150 cm, 100 cm and 50 cm above soil surface and without stake (control). The time of stem twining as subplots, i.e. stem twined early, twined at the time of 4 leaves stage, and twined at 8 leaves stage. Each combination of the treatments was replicated three times. The results showed that there was significant effect on the stake length  and the time of stem twining treatments on the plant growth and yield. The stake  length of 150 cm treatment produced the highest fresh tuber and total plant dry weight, i.e., 257.24 g and 132.77 g per plant, respectively. On the contrary, the plant without stake produced the lowest fresh tuber and  total dry weight of plant, i.e., 112.10 g and 48.65 g per plant, respectively.<br /><br />Keywords: biomass,leaf area, photosyntesis, tuber weight


Author(s):  
Riani Dwi Utari ◽  
Mohammad Masykuri ◽  
Retno Rosariastuti

There are many of rice field which is located near the industrial area. The industrial waste contains heavy metals (chromium) which will cause contamination of rice if the waste isn’t treated properly. The used of chemical fertilizers can cause chromium contamination. It needs an effort to do remediation, such as using phytostabilization mechanism. This research aimed to determine the role of chelator in chromium phytostabilization and its influence on the growth and quality of rice. The study was conducted in Waru village, Karanganyar in May to October 2018.  This research was factorial design used completely randomized block design with two factors, namely chemical fertilizers and chelator (<em>Agrobacterium </em>sp. I<sub>26</sub>, and manure). The parameters observed are chromium content and uptake by plant tissues (roots, shoot and rice), plant height and a number of clumps. Research output was without chemical fertilizer-chelator <em>Agrobacterium </em>sp. I<sub>26</sub> that can increase the chromium uptake in roots as big as 95.38 %, increased up to 10 %  in the shoot and decreased up to 92.38 % in rice compared to control. Application of <em>Agrobacterium</em> sp. I<sub>26</sub> can be recommended to produce good quality and quantity of rice (good growth and free from harmful pollutants such as chromium metal).


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