scholarly journals PECULIARITIES OF THE REGULATION OF THE IMMUNE SYSTEM IN CHILDREN RESIDING IN CONDITIONS OF STRONTIUM GEOCHEMICAL PROVINCE

2018 ◽  
Vol 97 (1) ◽  
pp. 25-29
Author(s):  
D. G. Dianova ◽  
Oleg V. Dolgikh ◽  
A. V. Krivtsov ◽  
O. O. Sinitsyna ◽  
E. A. Otavina
Keyword(s):  
Drinking Water ◽  
Immune System ◽  
Annexin V ◽  
Well Being ◽  
Reference Level ◽  
Control Group ◽  
Strontium Content ◽  
Blood Content ◽  
Stable Strontium ◽  
Geochemical Province

There was made a study of the features of apoptosis in children drinking potable water with an increased content of stable strontium of natural origin. The percentage of non-standard samples of strontium content in the water on the observation territory was 16.7%, which corresponded to the exceedance of the maximum permissible concentration of strontium in drinking water at the level of 1.2 MPC (up to 8.4 mg/l). In children living in the strontium geochemical province, the blood content of strontium (0.1 mg/dm3) was established statistically significant higher (p = 0.02) in comparison with the results obtained from children living in the territory of relative sanitary and hygienic well-being (0.028 mg/dm3). There was made an estimation of parameters of the dependence between the concentration of strontium in drinking water and the concentration of strontium in the blood was estimated, there was obtained a reliable dependence (b0 = 0.04, b1 = 0.012, R2 = 0.44, F = 310, p = 0.00001) of the increase in the blood content of strontium by the 1.3 times relative to the reference level from the concentration of strontium in water. In the exposure range of 7.84 ± 0.62 mg/L per 1 mg/L increase in strontium in water, its concentration in the blood was established to increase by 0.012 mg/dm3. In children living in the strontium geochemical province (1.5-0.8 times) by the method of flow cytometry, there were established to statistically significant decline the (relative and absolute values) number of CD95+ lymphocytes (p = 0.04-0.001) by 3.8 times, the expression of p53, bcl-2, Bax and the amount of Annexin V-FITC+PI- cells dying via the path of apoptosis, as well as the content of Annexin V-FITC+PI+ cells, dying via necrosis was statistically significantly (p = 0.001) elevated, in comparison with the control group. The obtained results prove the prolonged excessive effect on the children’s organism of stable strontium supplied with drinking water to lead to changes in the functioning of the immune system, characterized by a violation of the regulation of cell death - inhibition of apoptosis and activation of necrosis. The ranges of values of strontium in the blood corresponding to the ranges at the level of 0.1 [0.04-0.11] mg/dm3 are given in the ranges of apoptotic regulation (relative content,%): CD95+ - 17.50 [15.48; 22.03], p53 - 0.53 [0.22; 0.98], bcl-2 0.24 [0.21; 0.36], bax = 5.66 [4.67; 7.84], AnnexinV-FITC+PI- cells: 0.67 [0.56; 0.88], AnnexinV-FITC+ PI+ cells: 10.58 [9.54; 16.11]. The indices of apoptotic regulation of CD95+, p53, bcl-2, Annexin V-FITC+ PI- are recommended to be used as indices of early disorders of the immune status, and also for the monitoring the effectiveness of preventive measures in the territories of strontium geochemical provinces.

scholarly journals СHANGES OF IMMUNOREGULATORY INDEXES IN CHILDREN EXPOSED TO STRONTIUM

2018 ◽  
Vol 20 (6) ◽  
pp. 899-904
Author(s):  
E. A. Otavina ◽  
O. V. Dolgikh ◽  
K. G. Starkova ◽  
O. A. Kazakova ◽  
A. A. Mazunina
Keyword(s):  
Drinking Water ◽  
Immune Regulation ◽  
Comparison Group ◽  
Fold Increase ◽  
Immune Defense ◽  
Phagocytic Index ◽  
Control Group ◽  
Observation Group ◽  
Strontium Content ◽  
Humoral Factors

Evaluation of the immune system parameters can be used in order to assess capacity to adapt under conditions of increased external chemical load, including exposure to metals, which can exert either activating and inhibitory effects upon immune regulation parameters. The aim of this work was the analysis of immunoregulatory markers in a children’s population who consumed water with high strontium content (a sample from the Perm region). We carried out immunological evaluation of the children aged 7 to 12 years, living at a territory with a high strontium content in the drinking water. The comparison group included children from the conventionally clean region. We studied differential changes in cellular immunity (phagocytosis rates), humoral factors of immune defense (serum immunoglobulins), development of specific sensitization for strontium, as well as the processes of apoptosis triggering and regulation. A 3.68-fold increase in strontium levels was shown in fresh water within observation area, and the average  blood strontium content in the children of appropriate observation group was 1.55-fold higher than in children of the comparison group. At the same time, 1.2-fold increase in phagocytic activity determined as phagocytic number and phagocytic index was found, as compared to the control group. In 80% of the subjects, a reduction in serum IgG level was observed when compared to physiological norm, as well as a significant decrease in IgG and enhance in IgM production against the levels found in the comparison group. Wehave also shown an enhanced total sensitization in 55.0% of the observation group as shown by the total IgE test compared with normal age ranges, as well as excessive specific sensitization to strontium by 2.49 times, according to the IgG criterion. Disturbance in apoptosis triggering was associated with decreased number of CD95+ lymphocytes and TNFR1+ cells (2.8-fold compared to reference values), shifted balance in apoptogenic proteins, an average of 2.6-fold decrease in Bcl-2 expression, a 2.8-fold reduction of the p53 transcription factor expression relative to the reference interval. Thus, we have shown an ability of strontium excess in drinking water to influence the most important indices of immune regulation in pediatric population. These changes may serve as indices of populational health status under of external strontium exposure.

scholarly journals Cell death indicators in children under exposure to excessive intake of chlorine compounds with drinking water

2017 ◽  
pp. 80-87
Author(s):  
D Dianova ◽  
N Zaitseva ◽  
O Dolgikh ◽  
A Krivtsov
Keyword(s):  
Cell Death ◽  
Drinking Water ◽  
Water Supply ◽  
Glutathione Transferase ◽  
Annexin V ◽  
Fold Increase ◽  
Water Disinfection ◽  
Control Group ◽  
Domestic Water ◽  
Domestic Water Supply

Objective. The study of the immune status features including cell death markers in children consuming drinking water with excessive content of chlorine compounds. Materials and methods. Including the control group, 498 pre-school children were surveyed. The control group comprised 212 children living on territories where circulating groundwater is used as a source of domestic water supply. The study group consisted of 286 children living on territories with sources of domestic water supply presented by surface water of low quality due to microbiological indicators, which calls for the use of chlorine-containing agents for water disinfection. To conduct the study the chemical-analytical, immunological and statistical methods were used. Results. It was revealed that in the blood of children consuming hyperclorinated water, the concentration of chloroform in a range of 0 mg/dm3 - 0.2300 mg / dm3 has been identified, meanwhile in the biological medium of the control group children, the chloroform has not been detected. Using flow cytometry the statistically significant (р = 0.001) decrease (up to 1.7 fold) of Annexin V-FITC +PI- -cells amount (early apoptosis) and up to 2.4 fold increase (р = 0.001) of Annexin V-FITC+PI+ -cells content (late apoptosis / necrosis) was found in those children, who were exposed to the excessive content of chloroform with drinking water, relative to the values fixed in non-exposed children. Conclusions. It was demonstrated that under the increased chlorine compounds concentrations in blood the probable mechanism of apoptotic effect was related to the enzyme systems activation, associated with glutathione peroxidase, glutathione transferase, superoxide dismutase. The reference concentrations for chlorine-containing compounds in blood were proved by the criteria of IL6 and CD4+ -, CD8+ -, CD19+ -, CD25+ - lymphocytes content and their acceptable concentration range in blood (0.00001 mg / dm3 - 0.0181 mg / dm3) was defined.

scholarly journals Analysis of Pain and Analgesia Protocols in Acute Cerulein-Induced Pancreatitis in Male C57BL/6 Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Mattea Durst ◽  
Theresia Reding Graf ◽  
Rolf Graf ◽  
Mareike Kron ◽  
Margarete Arras ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Drinking Water ◽  
Acute Pain ◽  
Companion Animals ◽  
Inflammatory Cells ◽  
Well Being ◽  
Control Group ◽  
Experimental Mouse ◽  
Corticosterone Metabolites

Pancreatitis is known to be painful in humans and companion animals. However, the extent of pain in experimental mouse models of acute pancreatitis is unknown. Consequently, the severity classification of acute pancreatitis in mice is controversially discussed and standardized pain management is missing. In this study, we investigated acute Cerulein-induced pancreatitis with pain-specific and well-being orientated parameters to detect its impact on mice. Male C57BL/6J male mice were injected with Cerulein; animals that received saline injections served as control group. The animals were observed for weight change and water intake. To assess pain, behaviors like stretch-and-press and reduced rearing, the Mouse Grimace Scale, and von Frey hypersensitivity were assessed. Fecal corticosterone metabolites and burrowing behavior were assessed to detect changes in the animal’s well-being. Pancreatitis severity was evaluated with amylase and lipase in the blood and pancreas histology. To investigate whether different analgesics can alleviate signs of pain, and if they influence pancreas inflammation, animals received Buprenorphine, Paracetamol in combination with Tramadol, or Metamizole in the drinking water. The calculated intake of these analgesics via drinking reached values stated to be efficient for pain alleviation. While pancreatitis did not seem to be painful, we detected acute pain from Cerulein injections that could not be alleviated by analgesics. The number of inflammatory cells in the pancreas did not differ with the analgesic administered. In conclusion: (1) Cerulein injections appear to be acutely painful but pain could not be alleviated by the tested analgesics, (2) acute pancreatitis induced by our protocol did not induce obvious signs of pain, (3) analgesic substances had no detectable influence on inflammation. Nevertheless, protocols inducing more severe or even chronic pancreatitis might evoke more pain and analgesic treatment might become imperative. Considering our results, we recommend the use of Buprenorphine via drinking water in these protocols. Further studies to search for efficient analgesics that can alleviate the acute pain induced by Cerulein injections are needed.

scholarly journals Biomarkers of cytogenetic disorders under the external environmental isolated exposure of manganese and stable strontium from drinking water

2019 ◽  
Vol 95 (1) ◽  
pp. 102-105
Author(s):  
M. V. Karpova ◽  
Marina A. Zemlyanova ◽  
D. L. Mazunina
Keyword(s):  
Drinking Water ◽  
Cell Population ◽  
Genetic Instability ◽  
Proliferative Activity ◽  
Mutagenic Effect ◽  
Dna Oxidation ◽  
Reference Level ◽  
Stable Strontium ◽  
Oxidative Responses

In blood of children underwent to the long-term isolated exposure of manganese and stable strontium with drinking water exceeding the acceptable level by up to 1.4 times the elevated concentrations of mentioned metals in blood were shown to be formed in relation to reference level by 2,0-4,4 times. The developing at that mutagenic effect was proved to have the dependence on the level of manganese and stable strontium concentration in blood and is pronounced in the increased rate of cells with cytogenetic anomalies on the background of the enhancement of the proliferative activity, destructive changes in the cell population and oxidative responses at the cell DNA level. The activity of genetic instability and processes of DNA oxidation in the somatic cells in children under the exposure to manganese has more pronounced character in comparison to given indices under the exposure to the stable strontium.

scholarly journals The disorders of physical development of children residing in the conditions of low-level contamination of the atmospheric air and drinking water by metals (lead, manganese, nickel, chrome, cadmium) on the example of the Perm region

2019 ◽  
Vol 96 (1) ◽  
pp. 70-75
Author(s):  
Konstantin P. Luzhetskiy ◽  
O. Yu. Ustinova ◽  
A. Yu. Vandysheva ◽  
S. A. Vekovshinina
Keyword(s):  
Nervous System ◽  
Drinking Water ◽  
Negative Impact ◽  
Physical Development ◽  
Reference Level ◽  
Autonomous Nervous System ◽  
Blood Content ◽  
Complex Load ◽  
Atmospheric Air ◽  
Low Level

There was executed a clinical laboratory study of 183 children residing in the conditions of constant low-level impact of a complex of such consumed from atmospheric air and drinking water metals as lead, manganese, nickel, chrome, cadmium) and 46 children residing in the conditions of sanitary-hygienically wellbeing of the habitat. The evaluation of Total Hazard Indices (THI) for conditions of chemical substances entering with atmospheric air and drinking water have revealed the excess of accepted values (THI>1) regarding to disorders of the central nervous system in children (THI amounted for up to 4.93) and disorders ofendocrine system (up to THI of 1.13). The results of chemical-analytical researches showed the blood content of lead, manganese, nickel, chrome, cadmium in children living in conditions of low-dose complex load to exceed the reference level by 1.3-2.2 times. In persistent multi-medium polymetallic load the number of children having physical development disorders was found to increase up to 1.2-1.6 times (body weight deficiency - up to 16.5%). There is determined a verified direct probabilistic relationship of cause and effect ofphysical development disorders in children with high blood content of manganese, nickel, and chrome. Exceeding content of lead, manganese, nickel, and chrome (1.3-9.4 times higher than the reference level and the comparative group) in bio-media of children makes a negative impact on the central and autonomous nervous system, creates disorders in protein-synthetic processes in liver, form early shifts of hypothalamo-hypophyseal regulation with further deterioration of physical development indices.

scholarly journals Osteopathy incidence in children and adolescents consuming drinking water with high content of natural strontium

2021 ◽  
Vol 100 (10) ◽  
pp. 1123-1127
Author(s):  
Irina E. Shtina ◽  
Olga A. Maklakova ◽  
Svetlana L. Valina ◽  
Olga Y. Ustinova
Keyword(s):  
Bone Mineral Density ◽  
Drinking Water ◽  
Children And Adolescents ◽  
Bone Mineral ◽  
Comparison Group ◽  
Ionized Calcium ◽  
Observation Group ◽  
Mineral Density ◽  
Strontium Content ◽  
Geochemical Province

Introduction. Excessive intake of heavy metals of natural origin with drinking water increases the risk of the formation of non-infectious diseases of systems and organs, depending on the tropism of the chemical compound. The aim is to study the incidence of disorders of bone density and structure (osteopathies) in children and adolescents who consume drinking water with an increased strontium content due to the geochemical characteristics of the territory. Materials and methods. The observation group consisted of 93 children living in a geochemical province with a stable strontium content of more than 2 MPC in the centralized drinking water supply system. The comparison group included 148 children living in an area where strontium concentration in drinking water does not exceed 1 MPC. The incidence evaluation was carried out according to the official data of statistical materials. The analysis of the average values of the concentration in the blood of strontium, ionized calcium, inorganic phosphorus and alkaline phosphatase activity and the frequency of deviations from the norm of the studied parameters was carried out in the studied groups. The value of bone mineral density (Z-index) was determined according to the results of ultrasonic densitometry. Results. The level of long-term incidence of osteopathies for the period 2010-2019 in children and adolescents, the observation area was 6.59 ± 1.32 and 11.26 ± 2.07 ‰, respectively, which was 1.8 and 2.0 times higher than the level of the comparison territory (p = 0.0003-0.0004 ). The concentration of strontium in the blood of children living in the territory of the geochemical province exceeded 2.1 times the permissible reference (p <0.001) and 2.9 times the index of the comparison group (p = 0.0001). In ¾ children of the observation group, increased content of strontium in the blood was recorded, which was 5.2 times more often than the comparison area (p <0.001). In 57% of cases, the children of the observation group had a reduced level of ionized calcium in the blood (p = 0.0001). Low bone mineral density (Z-index) in the observation group occurred 2.3 times more often (17.2 versus 7.4%, p = 0.019). Significant dependences were established by lowering the level of ionized calcium and the value of the Z-index with increasing strontium content in the blood. Conclusion. In children living in geochemical provinces with an elevated strontium content, an increase in the incidence of osteopathy was noted, manifested by a reduced level of ionized calcium in the blood and low bone mineral density, which must be taken into account for the timely diagnosis and prevention of these conditions.

scholarly journals ANALYSIS OF THE EFFECTIVENESS OF TECHNOLOGIES FOR CORRECTING DISORDERS OF THE PHYSICAL DEVELOPMENT IN CHILDREN LIVING IN LOW-LEVEL ATMOSPHERIC AIR POLLUTION AND DRINKING WATER WITH METALS (LEAD, MANGANESE, NICKEL, CHROMIUM, CADMIUM)

2018 ◽  
Vol 97 (1) ◽  
pp. 75-81
Author(s):  
Konstantin P. Luzhetsky ◽  
O. Yu. Ustinova ◽  
O. I. Goleva ◽  
I. E. Shtina
Keyword(s):  
Drinking Water ◽  
High Efficiency ◽  
Endocrine System ◽  
Well Being ◽  
Physical Development ◽  
Reference Level ◽  
Metal Compounds ◽  
Atmospheric Air ◽  
Low Level ◽  
The Impact

In the course of studying the impact of metal compounds on the functioning of the endocrine system and adaptation systems in children living in chronic low-level multi-environmental (atmospheric air, drinking water) effects of a complex of metals, the elevated content of lead, manganese, nickel, cadmium and chromium was established to be 1.5 - 9.4 times higher than the reference level and 1.3 - 2.2 times than indices of the comparison group). In this contingent, disorders of the physical development and malnutrition (ICD: E44-46) were detected by 1.2 - 1.7 times more often than in conditions of sanitary and hygienic well-being. In the course of the analysis of the effectiveness of methods of correction in children of physical development disorders and malnutrition (E44-46) associated with the effects of metals, there was shown the high efficiency of the complex application of elimination, membrane stabilizing, antioxidant and nootropic technologies, with methods of physiotherapy (Ultrasound therapy, inductothermy) and exercise therapy. With comparable economic costs with traditional approaches, the proposed correction technologies demonstrate a significant benefit (up to 3.3 times) for the economy of the country and the region. The prevented losses for Gross Domestic Product (GDP) for the observation group are 13246.0 rubles per person per year (2.25 rubles per 1 ruble of costs, in contrast to 0.7 rubles when using standard methods).

scholarly journals Open-Label Placebo Interventions With Drinking Water and Their Influence on Perceived Physical and Mental Well-Being

2021 ◽  
Vol 12 ◽  
Author(s):  
Marco Rathschlag ◽  
Stefanie Klatt
Keyword(s):  
Drinking Water ◽  
Pain Treatment ◽  
Well Being ◽  
Post Treatment ◽  
The Other ◽  
Control Group ◽  
Treatment Level ◽  
Open Label ◽  
Performance Capability ◽  
Mental Well Being

In recent years, the postulation that deception is necessary for placebos to have an effect on pain relief or increased well-being has come into question. Latest studies have shown that an openly administered mock drug works just as well as a deceptively administered placebo on certain complaints. This open-label placebo effect has primarily been used in the area of pain treatment so far. This study is the first to examine the effect of such placebos on healthy individuals with the use of drinking water. In two experiments, participants were required to use certain specified water bottles for their daily drinking water consumption. At the beginning of Experiment 1, all participants (N = 68) received one bottle of water, which they were asked to refill themselves each day during a 2-week intervention period. In Experiment 2, participants (N = 75) received a new sealed water bottle every day. In both experiments, participants were randomly assigned to one of four groups: no treatment (control group CG), open-label placebo without rationale (OPR–), open-label placebo with rationale (OPR+), and open-label placebo with additional rationale in a suggested relaxed state (group OPR++). We conducted baseline and post-treatment measurements of the subjective perceived physical and mental well-being of the participants. In Experiment 1, only the OPR++ group reported enhanced vitality at the post-treatment level compared to the other groups. In Experiment 2, post-treatment measurements showed improvements for the OPR++ group in the Physical Performance Capability, Mental Performance Capability, Emotional Balance, Overall Recovery, Negative Emotional State, and Overall Stress categories compared to the other groups. Our results support the idea that placebos with an additional rationale in a suggestive relaxed state are more effective than with just a rationale in a normal state. Furthermore, our study shows the tendency that OLP++ in the form of water with health claims may be more effective when the water is given in several sealed bottles separately than in one sealed but refillable bottle.

A Pilot Study on the Efficacy of Volunteer Mentorship for Young Adults With Self-Harm Behaviors Using a Quasi-Experimental Design

Crisis ◽  
2016 ◽  
Vol 37 (6) ◽  
pp. 415-426 ◽  
Author(s):  
Yik-Wa Law ◽  
Paul S. F. Yip ◽  
Carmen C. S. Lai ◽  
Chi Leung Kwok ◽  
Paul W. C. Wong ◽  
...  
Keyword(s):  
Experimental Design ◽  
Suicide Ideation ◽  
Intervention Group ◽  
Well Being ◽  
Control Group ◽  
Treatment As Usual ◽  
Prevention Services ◽  
Quasi Experimental ◽  
Self Harm ◽  
Volunteer Support

Abstract. Background: Studies have shown that postdischarge care for self-harm patients is effective in reducing repeated suicidal behaviors. Little is known about whether volunteer support can help reduce self-harm repetition and improve psychosocial well-being. Aim: This study investigated the efficacy of volunteer support in preventing repetition of self-harm. Method: This study used a quasi-experimental design by assigning self-harm patients admitted to the emergency departments to an intervention group with volunteer support and treatment as usual (TAU) for 9 months and to a control group of TAU. Outcome measures include repetition of self-harm, suicidal ideation, hopelessness, and level of depressive and anxiety symptoms. Results: A total of 74 cases were recruited (38 participants; 36 controls). There were no significant differences in age, gender, and clinical condition between the two groups at the baseline. The intervention group showed significant improvements in hopelessness and depressive symptoms. However, the number of cases of suicide ideation and of repetition of self-harm episodes was similar for both groups at the postintervention period. Conclusion: Postdischarge care provided by volunteers showed significant improvement in hopelessness and depression. Volunteers have been commonly involved in suicide prevention services. Further research using rigorous methods is recommended for improving service quality in the long term.

The Effects of a Caffeine Placebo and Experimenter Expectation on Blood Pressure, Heart Rate, Well-Being, and Cognitive Performance

2001 ◽  
Vol 6 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Harald Walach ◽  
Stefan Schmidt ◽  
Yvonne-Michelle Bihr ◽  
Susanne Wiesch
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Cognitive Task ◽  
Well Being ◽  
Control Group ◽  
Double Blind ◽  
Main Effect ◽  
The Difference ◽  
Being There ◽  
To Receive

We studied the effect of experimenter expectations and different instructions in a balanced placebo design. 157 subjects were randomized into a 2 × 4 factorial design. Two experimenters were led to expect placebos either to produce physiological effects or not (pro- vs. antiplacebo). All subjects except a control group received a caffeine placebo. They were either made to expect coffee, no coffee, or were in a double-blind condition. Dependent measures were blood pressure, heart rate, well-being, and a cognitive task. There was one main effect on the instruction factor (p = 0.03) with the group “told no caffeine” reporting significantly better well-being. There was one main effect on the experimenter factor with subjects instructed by experimenter “proplacebo” having higher systolic blood pressure (p = 0.008). There was one interaction with subjects instructed by experimenter “proplacebo” to receive coffee doing worse in the cognitive task than the rest. Subjects instructed by experimenter “antiplacebo” were significantly less likely to believe the experimental instruction, and that mostly if they had been instructed to receive coffee. Contrary to the literature we could not show an effect of instruction, but there was an effect of experimenters. It is likely, however, that these experimenter effects were not due to experimental manipulations, but to the difference in personalities.

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