scholarly journals Analysis of Pain and Analgesia Protocols in Acute Cerulein-Induced Pancreatitis in Male C57BL/6 Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Mattea Durst ◽  
Theresia Reding Graf ◽  
Rolf Graf ◽  
Mareike Kron ◽  
Margarete Arras ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Drinking Water ◽  
Acute Pain ◽  
Companion Animals ◽  
Inflammatory Cells ◽  
Well Being ◽  
Control Group ◽  
Experimental Mouse ◽  
Corticosterone Metabolites

Pancreatitis is known to be painful in humans and companion animals. However, the extent of pain in experimental mouse models of acute pancreatitis is unknown. Consequently, the severity classification of acute pancreatitis in mice is controversially discussed and standardized pain management is missing. In this study, we investigated acute Cerulein-induced pancreatitis with pain-specific and well-being orientated parameters to detect its impact on mice. Male C57BL/6J male mice were injected with Cerulein; animals that received saline injections served as control group. The animals were observed for weight change and water intake. To assess pain, behaviors like stretch-and-press and reduced rearing, the Mouse Grimace Scale, and von Frey hypersensitivity were assessed. Fecal corticosterone metabolites and burrowing behavior were assessed to detect changes in the animal’s well-being. Pancreatitis severity was evaluated with amylase and lipase in the blood and pancreas histology. To investigate whether different analgesics can alleviate signs of pain, and if they influence pancreas inflammation, animals received Buprenorphine, Paracetamol in combination with Tramadol, or Metamizole in the drinking water. The calculated intake of these analgesics via drinking reached values stated to be efficient for pain alleviation. While pancreatitis did not seem to be painful, we detected acute pain from Cerulein injections that could not be alleviated by analgesics. The number of inflammatory cells in the pancreas did not differ with the analgesic administered. In conclusion: (1) Cerulein injections appear to be acutely painful but pain could not be alleviated by the tested analgesics, (2) acute pancreatitis induced by our protocol did not induce obvious signs of pain, (3) analgesic substances had no detectable influence on inflammation. Nevertheless, protocols inducing more severe or even chronic pancreatitis might evoke more pain and analgesic treatment might become imperative. Considering our results, we recommend the use of Buprenorphine via drinking water in these protocols. Further studies to search for efficient analgesics that can alleviate the acute pain induced by Cerulein injections are needed.

scholarly journals The effectiveness of Pectin in the reduction of histological changes caused by exposure to monosodium glutamate in female mice: مدى فعالية البكتين في الحد من التغيرات النسيجية التي يسببها تناول جلوتامات أحادي الصوديوم على إناث الفئران

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Sarah Ibrahim Al Othman, Faten khalif Alanazi, Ghada Jaber S
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Monosodium Glutamate ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Control Group ◽  
Central Vein ◽  
Histological Changes ◽  
Female Mice ◽  
Liver And Kidney

Monosodium glutamate (MSG) is widely used as a food additive. Excessive consumption of monosodium glutamate has also been shown to affect the liver and kidneys, causing damage to these tissues because of oxidative stress leading to increased production of reactive oxygen species (ROS). The purpose of the study described in this paper was to find out how the liver and kidney toxicity caused by monosodium glutamate can be mitigated using pectin. To this end, 30 albino mice females were divided into four groups. The animals were distributed in special cages. 12-15 weeks with an average body weight of 60 grams. The animals were divided into four groups: the experimental control group (1) comprising 5 female mice were given normal drinking water and the treated group (2) comprising 10 female mice were given monosodium glutamate at a dose of 3 g/kg body weight in drinking water. For three weeks, the treatment group (3) comprising 10 female mice was given pectin at a dose of 300 mg/70 kg body weight in drinking water immediately after the monosodium glutamate dose for three weeks and the pectin group (4) comprising 5 female mice were given Pectin at a dose of 300 mg/70 kg body weight in drinking water for three weeks. The mice were then anesthetized, dissected, and liver and kidney samples were taken from female mice and kept in a 10% neutral formalin solution to make tissue segments. The results showed many histological changes in the liver, such as congestion of the central vein, widening of the sinuses, and the appearance of signs of the death of most hepatocytes, infiltration of the central vein and an invasion of inflammatory cells around the central vein with the emergence of several gaps within the cells. Many of them cavity with the death of most of the tubule cells, the closure of some of them and the expansion and infiltration in others and bleeding inside the tissue. Pectin therapy has led to the disappearance of most of these changes and the emergence of a clear improvement in hepatic and renal tissue.

scholarly journals Common Biological Modulators of Acute Pain: An Overview Within the AAAPT Project (ACTTION-APS-AAPM Acute Pain Taxonomy)

Pain Medicine ◽  
2020 ◽  
Vol 21 (10) ◽  
pp. 2394-2400
Author(s):  
Michele Curatolo
Keyword(s):  
Chronic Pain ◽  
Acute Pain ◽  
Pain Sensitivity ◽  
Surgical Techniques ◽  
Well Being ◽  
Opioid Consumption ◽  
Screening Tools ◽  
Opioid Use ◽  
Acute Low Back Pain

Abstract Objective The ACTTION-APS-AAPM Acute Pain Taxonomy (AAAPT) project relies on the identification of modulators to improve characterization and classification of acute pain conditions. In the frame of the AAAPT effort, this paper presents an overview of common biological modulators of acute pain. Methods Nonsystematic overview. Results Females may experience more acute pain than males, but the clinical significance may be modest. Increasing age is associated with decreasing analgesic requirement and decreasing pain intensity after surgery and with higher risk of acute low back pain. Racial and ethnic minorities have worse pain, function, and perceived well-being. Patients with preexisting chronic pain and opioid use are at higher risk of severe acute pain and high opioid consumption. The OPRM1 gene A118G polymorphism is associated with pain severity and opioid consumption, with modest quantitative impact. Most studies have found positive associations between pain sensitivity and intensity of acute clinical pain. However, the strength of the association is unclear. Surgical techniques, approaches, and complications influence postoperative pain. Conclusions Sex, age, race, ethnicity, preexisting chronic pain and opioid use, surgical approaches, genetic factors, and pain sensitivity are biological modulators of acute pain. Large studies with multisite replication will quantify accurately the association between modulators and acute pain and establish the value of modulators for characterization and classification of acute pain conditions, as well as their ability to identify patients at risk of uncontrolled pain. The development and validation of quick, bed-side pain sensitivity tests would allow their implementation as clinical screening tools. Acute nonsurgical pain requires more investigation.

scholarly journals PECULIARITIES OF THE REGULATION OF THE IMMUNE SYSTEM IN CHILDREN RESIDING IN CONDITIONS OF STRONTIUM GEOCHEMICAL PROVINCE

2018 ◽  
Vol 97 (1) ◽  
pp. 25-29
Author(s):  
D. G. Dianova ◽  
Oleg V. Dolgikh ◽  
A. V. Krivtsov ◽  
O. O. Sinitsyna ◽  
E. A. Otavina
Keyword(s):  
Drinking Water ◽  
Immune System ◽  
Annexin V ◽  
Well Being ◽  
Reference Level ◽  
Control Group ◽  
Strontium Content ◽  
Blood Content ◽  
Stable Strontium ◽  
Geochemical Province

There was made a study of the features of apoptosis in children drinking potable water with an increased content of stable strontium of natural origin. The percentage of non-standard samples of strontium content in the water on the observation territory was 16.7%, which corresponded to the exceedance of the maximum permissible concentration of strontium in drinking water at the level of 1.2 MPC (up to 8.4 mg/l). In children living in the strontium geochemical province, the blood content of strontium (0.1 mg/dm3) was established statistically significant higher (p = 0.02) in comparison with the results obtained from children living in the territory of relative sanitary and hygienic well-being (0.028 mg/dm3). There was made an estimation of parameters of the dependence between the concentration of strontium in drinking water and the concentration of strontium in the blood was estimated, there was obtained a reliable dependence (b0 = 0.04, b1 = 0.012, R2 = 0.44, F = 310, p = 0.00001) of the increase in the blood content of strontium by the 1.3 times relative to the reference level from the concentration of strontium in water. In the exposure range of 7.84 ± 0.62 mg/L per 1 mg/L increase in strontium in water, its concentration in the blood was established to increase by 0.012 mg/dm3. In children living in the strontium geochemical province (1.5-0.8 times) by the method of flow cytometry, there were established to statistically significant decline the (relative and absolute values) number of CD95+ lymphocytes (p = 0.04-0.001) by 3.8 times, the expression of p53, bcl-2, Bax and the amount of Annexin V-FITC+PI- cells dying via the path of apoptosis, as well as the content of Annexin V-FITC+PI+ cells, dying via necrosis was statistically significantly (p = 0.001) elevated, in comparison with the control group. The obtained results prove the prolonged excessive effect on the children’s organism of stable strontium supplied with drinking water to lead to changes in the functioning of the immune system, characterized by a violation of the regulation of cell death - inhibition of apoptosis and activation of necrosis. The ranges of values of strontium in the blood corresponding to the ranges at the level of 0.1 [0.04-0.11] mg/dm3 are given in the ranges of apoptotic regulation (relative content,%): CD95+ - 17.50 [15.48; 22.03], p53 - 0.53 [0.22; 0.98], bcl-2 0.24 [0.21; 0.36], bax = 5.66 [4.67; 7.84], AnnexinV-FITC+PI- cells: 0.67 [0.56; 0.88], AnnexinV-FITC+ PI+ cells: 10.58 [9.54; 16.11]. The indices of apoptotic regulation of CD95+, p53, bcl-2, Annexin V-FITC+ PI- are recommended to be used as indices of early disorders of the immune status, and also for the monitoring the effectiveness of preventive measures in the territories of strontium geochemical provinces.

scholarly journals Open-Label Placebo Interventions With Drinking Water and Their Influence on Perceived Physical and Mental Well-Being

2021 ◽  
Vol 12 ◽  
Author(s):  
Marco Rathschlag ◽  
Stefanie Klatt
Keyword(s):  
Drinking Water ◽  
Pain Treatment ◽  
Well Being ◽  
Post Treatment ◽  
The Other ◽  
Control Group ◽  
Treatment Level ◽  
Open Label ◽  
Performance Capability ◽  
Mental Well Being

In recent years, the postulation that deception is necessary for placebos to have an effect on pain relief or increased well-being has come into question. Latest studies have shown that an openly administered mock drug works just as well as a deceptively administered placebo on certain complaints. This open-label placebo effect has primarily been used in the area of pain treatment so far. This study is the first to examine the effect of such placebos on healthy individuals with the use of drinking water. In two experiments, participants were required to use certain specified water bottles for their daily drinking water consumption. At the beginning of Experiment 1, all participants (N = 68) received one bottle of water, which they were asked to refill themselves each day during a 2-week intervention period. In Experiment 2, participants (N = 75) received a new sealed water bottle every day. In both experiments, participants were randomly assigned to one of four groups: no treatment (control group CG), open-label placebo without rationale (OPR–), open-label placebo with rationale (OPR+), and open-label placebo with additional rationale in a suggested relaxed state (group OPR++). We conducted baseline and post-treatment measurements of the subjective perceived physical and mental well-being of the participants. In Experiment 1, only the OPR++ group reported enhanced vitality at the post-treatment level compared to the other groups. In Experiment 2, post-treatment measurements showed improvements for the OPR++ group in the Physical Performance Capability, Mental Performance Capability, Emotional Balance, Overall Recovery, Negative Emotional State, and Overall Stress categories compared to the other groups. Our results support the idea that placebos with an additional rationale in a suggestive relaxed state are more effective than with just a rationale in a normal state. Furthermore, our study shows the tendency that OLP++ in the form of water with health claims may be more effective when the water is given in several sealed bottles separately than in one sealed but refillable bottle.

Pro-oxidant effects of a high α-tocopherol dose on kidney antioxidant biomarkers and histopathological aspects

2017 ◽  
Vol 87 (3-4) ◽  
pp. 179-190
Author(s):  
Amel Kanane ◽  
Fayrouz Rouaki ◽  
Mohamed Brahim Errahmani ◽  
Abdenour Laraba ◽  
Hayet Mesbah ◽  
...  
Keyword(s):  
Sunflower Oil ◽  
Antioxidant Status ◽  
Basal Diet ◽  
Inflammatory Cells ◽  
Significant Rise ◽  
Control Group ◽  
Histopathological Changes ◽  
Group 5 ◽  
Oxidant Effect ◽  
Higher Doses

Abstract. The aim of this study is to evaluate the effect of α-tocopherol supplementation at two doses (600 and 1200 mg × kg–1) on kidney antioxidant status and the histopathological changes in Wistar rats after 12 weeks of exposure at different diets. Forty rats has been divided into 4 groups of 10 rats each, the control group received basal diet with 5 % fresh sunflower oil (FSO), the second group: 5 % oxidized sunflower oil (OSO), the third group: 5 % OSO supplemented with 600 mg × kg–1 α-tocopherol and the fourth group: 5 % OSO supplemented with 1200 mg × kg–1 α-tocopherol. In OSO groups, the results showed highly significant increases of LPO (from 31.3 ± 0.9 to 53.8 ± 1.2 nmol of MDA formed/min/mg protein, p < 0.0001) with a significant decrease (p < = 0.001) of the antioxidant enzymatic activities (CAT, SOD, GPX, GR and G6PDH), body weight (339 ± 9 to 290 ± 3 g) and α-tocopherol levels (13.6 ± 0.6 to 6.5 ± 0.4 μg/mg protein). In OSO groups with 600 mg × kg–1 α-tocopherol, an antioxidant effect was found, reflected by a return of the parameters to values similar to those of the control group. However, higher doses of α-tocopherol (1200 mg × kg–1) induced a depletion of antioxidant status, α-tocopherol levels (6.0 ± 0.3 μg/mg protein, p < 0.001) and a very highly significant rise (p < 0.0001) of LPO content (54.86 ± 0.01 nmol of MDA formed/min/mg protein). The kidney tissues also showed changes in glomerular, severe inflammatory cells infiltration, and formation of novel vessels. So, we can conclude that the oxidative stress is attenuated by a moderate administration of 600 mg × kg–1 α-tocopherol, while a pro-oxidant effect occurs at 1200 mg × kg–1 α-tocopherol.

A Pilot Study on the Efficacy of Volunteer Mentorship for Young Adults With Self-Harm Behaviors Using a Quasi-Experimental Design

Crisis ◽  
2016 ◽  
Vol 37 (6) ◽  
pp. 415-426 ◽  
Author(s):  
Yik-Wa Law ◽  
Paul S. F. Yip ◽  
Carmen C. S. Lai ◽  
Chi Leung Kwok ◽  
Paul W. C. Wong ◽  
...  
Keyword(s):  
Experimental Design ◽  
Suicide Ideation ◽  
Intervention Group ◽  
Well Being ◽  
Control Group ◽  
Treatment As Usual ◽  
Prevention Services ◽  
Quasi Experimental ◽  
Self Harm ◽  
Volunteer Support

Abstract. Background: Studies have shown that postdischarge care for self-harm patients is effective in reducing repeated suicidal behaviors. Little is known about whether volunteer support can help reduce self-harm repetition and improve psychosocial well-being. Aim: This study investigated the efficacy of volunteer support in preventing repetition of self-harm. Method: This study used a quasi-experimental design by assigning self-harm patients admitted to the emergency departments to an intervention group with volunteer support and treatment as usual (TAU) for 9 months and to a control group of TAU. Outcome measures include repetition of self-harm, suicidal ideation, hopelessness, and level of depressive and anxiety symptoms. Results: A total of 74 cases were recruited (38 participants; 36 controls). There were no significant differences in age, gender, and clinical condition between the two groups at the baseline. The intervention group showed significant improvements in hopelessness and depressive symptoms. However, the number of cases of suicide ideation and of repetition of self-harm episodes was similar for both groups at the postintervention period. Conclusion: Postdischarge care provided by volunteers showed significant improvement in hopelessness and depression. Volunteers have been commonly involved in suicide prevention services. Further research using rigorous methods is recommended for improving service quality in the long term.

The Effects of a Caffeine Placebo and Experimenter Expectation on Blood Pressure, Heart Rate, Well-Being, and Cognitive Performance

2001 ◽  
Vol 6 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Harald Walach ◽  
Stefan Schmidt ◽  
Yvonne-Michelle Bihr ◽  
Susanne Wiesch
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Cognitive Task ◽  
Well Being ◽  
Control Group ◽  
Double Blind ◽  
Main Effect ◽  
The Difference ◽  
Being There ◽  
To Receive

We studied the effect of experimenter expectations and different instructions in a balanced placebo design. 157 subjects were randomized into a 2 × 4 factorial design. Two experimenters were led to expect placebos either to produce physiological effects or not (pro- vs. antiplacebo). All subjects except a control group received a caffeine placebo. They were either made to expect coffee, no coffee, or were in a double-blind condition. Dependent measures were blood pressure, heart rate, well-being, and a cognitive task. There was one main effect on the instruction factor (p = 0.03) with the group “told no caffeine” reporting significantly better well-being. There was one main effect on the experimenter factor with subjects instructed by experimenter “proplacebo” having higher systolic blood pressure (p = 0.008). There was one interaction with subjects instructed by experimenter “proplacebo” to receive coffee doing worse in the cognitive task than the rest. Subjects instructed by experimenter “antiplacebo” were significantly less likely to believe the experimental instruction, and that mostly if they had been instructed to receive coffee. Contrary to the literature we could not show an effect of instruction, but there was an effect of experimenters. It is likely, however, that these experimenter effects were not due to experimental manipulations, but to the difference in personalities.

Russian Citizens' Representations of the Country's Position in the Geopolitical Space of the Commonwealth of Independent States, Europe, and the World

2003 ◽  
Vol 8 (4) ◽  
pp. 238-251
Author(s):  
Victor F. Petrenko ◽  
Olga V. Mitina ◽  
Kirill A. Bertnikov
Keyword(s):  
Well Being ◽  
Multidimensional Space ◽  
Commonwealth Of Independent States ◽  
Repertory Grids ◽  
Implicit Model ◽  
The World ◽  
Public Consciousness ◽  
Independent States ◽  
Invariant Factors

The aim of this research was the reconstruction of the system of categories through which Russians perceive the countries of the Commonwealth of Independent States (CIS), Europe, and the world as a whole; to study the implicit model of the geopolitical space; to analyze the stereotypes in the perception of different countries and the superposition of mental geopolitical representations onto the geographic map. The techniques of psychosemantics by Petrenko, originating in the semantic differential of Osgood and Kelly's “repertory grids,” were used as working tools. Multidimensional semantic spaces act as operational models of the structures of consciousness, and the positions of countries in multidimensional space reflect the geopolitical stereotypes of respondents about these countries. Because of the transformation of geopolitical reality representations in mass consciousness, the commonly used classification of countries as socialist, capitalist, and developing is being replaced by other structures. Four invariant factors of the countries' descriptions were identified. They are connected with Economic and Political Well-being, Military Might, Friendliness toward Russia, and Spirituality and the Level of Culture. It seems that the structure has not been explained in adequate detail and is not clearly realized by the individuals. There is an interrelationship between the democratic political structure of a country and its prosperity in the political mentality of Russian respondents. Russian public consciousness painfully strives for a new geopolitical identity and place in the commonwealth of states. It also signifies the country's interest and orientation toward the East in the search for geopolitical partners. The construct system of geopolitical perception also depends on the region of perception.

Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Eman A. Al-Rekabi ◽  
Dheyaa K. Alomer ◽  
Rana Talib Al-Muswie ◽  
Khalid G. Al-Fartosi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Control Group ◽  
Very Low Density Lipoprotein ◽  
Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

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