scholarly journals Klebsiella pneumoniae: microbiological characterization, antimicrobial resistance, and virulence

2020 ◽  
Vol 23 (3) ◽  
pp. 191-197
Author(s):  
Olga V. Shamina ◽  
Ekaterina A. Samoylova ◽  
Irina E. Novikova ◽  
Anna V. Lazareva
Keyword(s):  
Antimicrobial Resistance ◽  
Literature Review ◽  
Klebsiella Pneumoniae ◽  
Infectious Diseases ◽  
Virulence Factors ◽  
Opportunistic Infections ◽  
Biological Properties ◽  
Review Literature ◽  
Causative Agents ◽  
Life Threatening

Klebsiella pneumoniae (KP) represents one of the main causative agents of opportunistic infections. KP associated infectious diseases can be local, for example, pneumonia, and generalized, including severe, often life-threatening conditions (meningitis, sepsis). Besides the chromosomal genome with a variety of different genetic loci, KP contains an additional plasmid genome which endues it with important biological properties. That’s why KP strains can be opportunistic, hypervirulent, and resistant to antimicrobials. In this literature review, literature data on the molecular resistant mechanisms, virulence factors and infectious diseases caused by KP is discussed.

scholarly journals A case of neck abscess caused by rare hypervirulent Klebsiella pneumoniae, capsular type K20 and sequence type 420

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
John Alexander McHardy ◽  
Vathshalan Selvaganeshapillai ◽  
Priya Khanna ◽  
Ashley Michael Whittington ◽  
Jane Turton ◽  
...  
Keyword(s):  
Public Health ◽  
Case Report ◽  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
East Asia ◽  
South East Asia ◽  
Neck Abscess ◽  
Global Challenge ◽  
Life Threatening ◽  
The Uk

Abstract Background This case report describes a neck abscess caused by a strain of Hypervirulent Klebsiella pneumoniae in a middle aged man with diabetes without a history of travel to East and South East Asia. This case report is of notable significance as Hypervirulent Klebsiella pneumoniae neck abscesses are rarely seen in the UK and are very infrequently documented in individuals who have not first travelled to the high prevalence areas of East and South East Asia. Case presentation This case report describes a 53 year old diabetic man who contracted a Hypervirulent Klebsiella pneumoniae neck abscess which led to the development of sepsis. Klebsiella pneumoniae was cultured from blood cultures and fluid aspirated from the abscess grew the pathogen with same antimicrobial susceptibility. Hypervirulence was demonstrated after the samples were analysed, at the Antimicrobial Resistance and Healthcare Associated Infections Reference Unit Public Health England Colindale, and found to contain the K20 (rmp)A and rmpA2 virulence genes. Discussion Hypervirulent Klebsiella pneumoniae is a Gram-negative, encapsulated, non-motile bacillus notable for its ability to metastatically spread and cause potentially life threatening infections in otherwise healthy adults, but especially in those with diabetes. Genes responsible for the production of hyperviscous mucoid polysaccharide capsules and siderophores, such as those isolated in this case, enable the bacteria to more efficiently evade the hosts immune system and disseminate and invade surrounding and distant tissues. Data from Public Health England shows Hypervirulent Klebsiella pneumoniae are rare in the UK. A review of current literature also showed Hypervirulent Klebsiella pneumoniae almost exclusively occur in those who have traveled to East and South East Asia. Conclusions This case reported a rare Hypervirulent Klebsiella pneumoniae neck abscess outside of, and without travel to, East and South East Asia. This raises concerns about future, potentially life threatening, Hypervirulent Klebsiella pneumoniae infections becoming more widespread without the need for endemic travel. This concern is further exacerbated by the growing global challenge of antimicrobial resistance.

scholarly journals Metabolite Transporter PEG344 Is Required for Full Virulence of Hypervirulent Klebsiella pneumoniae Strain hvKP1 after Pulmonary but Not Subcutaneous Challenge

2017 ◽  
Vol 85 (10) ◽  
Author(s):  
Jeffrey Bulger ◽  
Ulrike MacDonald ◽  
Ruth Olson ◽  
Janet Beanan ◽  
Thomas A. Russo
Keyword(s):  
Klebsiella Pneumoniae ◽  
Virulence Factors ◽  
Ex Vivo ◽  
Infection Model ◽  
Virulence Plasmid ◽  
Content Type ◽  
Model Studies ◽  
Life Threatening ◽  
Cd1 Mice

ABSTRACT Hypervirulent Klebsiella pneumoniae (hvKP) is an emerging pathotype that is capable of causing tissue-invasive and organ- and life-threatening infections in healthy individuals from the community. Knowledge on the virulence factors specific to hvKP is limited. In this report, we describe a new factor (PEG344) that increases the virulence of hvKP strain hvKP1. peg-344 is present on the hvKP1 virulence plasmid, is broadly prevalent among hvKP strains, and has increased RNA abundance when grown in human ascites. An isogenic derivative of hvKP1 (hvKP1Δpeg-344) was constructed and compared with its wild-type parent strain in in vitro, ex vivo, and infection model studies. Both survival and competition experiments with outbred CD1 mice demonstrated that PEG344 was required for full virulence after pulmonary challenge but, interestingly, not after subcutaneous challenge. In silico analysis suggested that PEG344 serves as an inner membrane transporter. Compared to hvKP1, a small but significant decrease in the growth/survival of hvKP1Δpeg-344 was observed in human ascites, but resistance to the bactericidal activity of complement was similar. These data suggested that PEG344 may transport an unidentified growth factor present in ascites. The data presented are important since they expand our limited knowledge base on virulence factors unique to hvKP, which is needed to lay the groundwork for translational approaches to prevent or treat these devastating infections.

scholarly journals DETECTION OF LISTERIA MONOCYTOGENES IN RAW MILK AND ABORTED COW CASES AT SALAHUDEEN PROVINCE

2021 ◽  
Vol 52 (2) ◽  
pp. 315-321
Author(s):  
B. S. Noomy ◽  
Sh. A. Anwar ◽  
S. M. Salih
Keyword(s):  
Listeria Monocytogenes ◽  
Infectious Diseases ◽  
Virulence Factors ◽  
Virulence Genes ◽  
Raw Milk ◽  
Isolation Rate ◽  
Milk Samples ◽  
Vaginal Swabs ◽  
Causative Agents

Listeria monocytogenes is a pathogen that causes infectious diseases in animals. It is one of the causal agents causing abortion in infected cows. This study was conducted to detect listeria monocytogenes in aborted cows. Also to estimate the role of the milk in the distribution of the pathogen by detecting the bacteria in milk of aborted cows as well as in raw milk from the market of Salahudeen province. The study includes 46 aborted cows from which 46 milk samples were taken to detect the causative agents. Also 38 vaginal swabs were taken from the same aborted cows and 8 samples from fetuses. 30 raw milk samples were also taken from market at Salahudeen province. The results showed that Listeria monocytogenes were detected in 5 (13.1%) of vaginal swabs, 2 (25%) of aborted fetuses, 13 (28.26%) in milks from aborted cows, and 9 (30%) of raw milks. The isolated pathogens were screened for the presence of 3 virulence factors; InlJ, InlA, and HIY. The results showed that theses virulence genes were found in the majority of the isolates and the isolation rate ranged between 75%-100%. The study concluded that milk is one of the main sources for the pathogen spreads to other animals.

scholarly journals Hybrid Assembly Provides Improved Resolution of Plasmids, Antimicrobial Resistance Genes, and Virulence Factors in Escherichia coli and Klebsiella pneumoniae Clinical Isolates

2021 ◽  
Vol 9 (12) ◽  
pp. 2560
Author(s):  
Abdolrahman Khezri ◽  
Ekaterina Avershina ◽  
Rafi Ahmad
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Blood Culture ◽  
Klebsiella Pneumoniae ◽  
Virulence Factors ◽  
Whole Genome Sequence ◽  
Clinical Samples ◽  
Hybrid Assembly ◽  
Antimicrobial Resistance Genes ◽  
Quality Checks

Emerging new sequencing technologies have provided researchers with a unique opportunity to study factors related to microbial pathogenicity, such as antimicrobial resistance (AMR) genes and virulence factors. However, the use of whole-genome sequence (WGS) data requires good knowledge of the bioinformatics involved, as well as the necessary techniques. In this study, a total of nine Escherichia coli and Klebsiella pneumoniae isolates from Norwegian clinical samples were sequenced using both MinION and Illumina platforms. Three out of nine samples were sequenced directly from blood culture, and one sample was sequenced from a mixed-blood culture. For genome assembly, several long-read, (Canu, Flye, Unicycler, and Miniasm), short-read (ABySS, Unicycler and SPAdes) and hybrid assemblers (Unicycler, hybridSPAdes, and MaSurCa) were tested. Assembled genomes from the best-performing assemblers (according to quality checks using QUAST and BUSCO) were subjected to downstream analyses. Flye and Unicycler assemblers performed best for the assembly of long and short reads, respectively. For hybrid assembly, Unicycler was the top-performing assembler and produced more circularized and complete genome assemblies. Hybrid assembled genomes performed substantially better in downstream analyses to predict putative plasmids, AMR genes and β-lactamase gene variants, compared to MinION and Illumina assemblies. Thus, hybrid assembly has the potential to reveal factors related to microbial pathogenicity in clinical and mixed samples.

Characterization of antimicrobial resistance patterns of Klebsiella pneumoniae isolates obtained from wound infections

2020 ◽  
Vol 20 ◽  
Author(s):  
Roya Ghanavati ◽  
Hossein Kazemian ◽  
Parisa Asadollahi ◽  
Hamid Heidari ◽  
Gholamreza Irajian ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
Control Strategies ◽  
Wound Infections ◽  
Aminoglycoside Resistance ◽  
Resistance Patterns ◽  
Life Threatening ◽  
Synergy Test ◽  
Aminoglycoside Resistance Genes

Background: Multidrug resistance among ESBL producing isolates has limited the administration of proper antibiotics. It is therefore important to monitor the resistance patterns of Klebsiella pneumoniae isolates and provide infection control strategies to prevent nosocomial outbreaks. This study was aimed to determine antimicrobial resistance patterns of K. pneumoniae isolates obtained from wound infections of patients in Tehran, Iran. Methods: Totally, 102 K. pneumoniae isolates were obtained from wound infections of patients in Tehran, Iran. Phenotypic ESBL and carbapenemase production was assessed using double-disc synergy test (DDST) and modified Hodge test (MHT), respectively. PCR was performed for the detection of ESBL, carbapenemase, quinolone and aminoglycoside resistance genes. Results: Forty-six (45.1%) and 23 (22.5%) isolates, out of the 102 isolates, were phenotypically detected as ESBL and carbapenemase producers, respectively. The PCR results showed that 80/102 (78.4%) and 51/102 (50%) isolates possessed at least one of the assessed ESBL and carbapenemase genes, respectively. Quinolone resistance determinants (QRDs) and aac(6')-Ib genes were found amongst 50 (49%) and 67 (65.7%) isolates, respectively. Four isolates carried the blaTEM, blaSHV, blaCTX-M, qnrB, qnrS and aac(6’)-Ib genes, simultaneously. Conclusion: Because of the presence of multiple resistance genes among some K. pneumoniae strains, antibiotic agents should be used with caution to preserve their efficacy in case of life-threatening infections.

scholarly journals Invasive liver abscess caused by hypervirulent Klebsiella pneumoniae: case report and literature review

2020 ◽  
Author(s):  
Xiao-Guang Xiao ◽  
Zhao-Hong Mu ◽  
Qing-Dong Li ◽  
Yong-Li Zhang
Keyword(s):  
Literature Review ◽  
Klebsiella Pneumoniae ◽  
Liver Abscess ◽  
Pathogenic Bacteria ◽  
Multiple Organ ◽  
Multiple Organ Dysfunction ◽  
Antimicrobial Drugs ◽  
Case Presentation ◽  
Life Threatening

Abstract Background: Klebsiella pneumoniae is a common pathogenic bacteria that cause infectious diseases, Klebsiella pneumoniae invasive liver abscess syndrome is a rare disease, which often caused by hypervirulent Klebsiella pneumoniae(hvKP), the hvKP patients with sepsis may have life-threatening multiple organ dysfunction and visual sequelae.Case presentation: We report a case of Klebsiella pneumoniae invasive liver abscess syndrome caused by hvKP, the patient was treated with long-term antibiotic treatment, along with controlling blood sugar with Insulin, and the patient was eventually discharged from the hospital. Case analysis and literature review were performed to improve the understanding of invasive liver abscess caused by hvKP.Conclusions Patients with hvKP invasive liver abscess are in relatively severe conditions and often have poor visual prognosis. Appropriate antimicrobial drugs should be administered to prevent complications and improve prognosis.

scholarly journals Virulence Factors, Capsular Serotypes and Antimicrobial Resistance of Hypervirulent Klebsiella pneumoniae and Classical Klebsiella pneumoniae in Southeast Iran

2019 ◽  
Vol 51 ◽  
Author(s):  
Sanaz Rastegar ◽  
Mohammad Moradi ◽  
Davood Kalantar-Neyestanaki ◽  
Ali Golabi dehdasht ◽  
Hossein Hosseini-Nave
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Virulence Factors

scholarly journals STATE OF ANTIBIOTICS RESISTANCE OF KLEBSIELLA PNEUMONIAE

2017 ◽  
pp. 70-77 ◽  
Author(s):  
E. V. Anganova ◽  
A. V. Vetokhina ◽  
L. A. Raspopina ◽  
E. L. Kichigina ◽  
E. D. Savilov
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
International Collaboration ◽  
Antibiotics Resistance ◽  
Antibacterial Resistance ◽  
Carbapenem Resistant ◽  
Causative Agents ◽  
Clinically Significant ◽  
High Level

Klebsiella pneumoniae microorganisms belong to a group of the most prevalent clinically significant pathogens with a high level of antibacterial resistance (ESKAPE). The speed of formation of antibiotics-resistance by K. pneumoniae strains has sharply increased and reached pandemic scale. One of the main clinically significant mechanisms of antimicrobial resistance is (3-lactamase production, the groups being active depending on the region, country' and hospital. Currently, a significant part of nosocomial K. pneumoniae is resistant to penicillins, 11I-IV generation cephalosporins. The growth of resistance of klebsiellae to carbapenems is a serious threat to the healthcare system. First ofall, KPC-, OXA-, NDM-, VIM-, IMP-producing Widespread of carbapenem-resistant klebsiellae gives evidence on the necessity of international collaboration within the framework of antibiotics resistance control. An increase of frequency of obtained resistance of K. pneumoniae to non-(3-lactame antibiotics (fluoroquinolones, aminoglycosides) is noted. Isolates of K. pneumoniae resistant to tygecyclin, colistin are registered. In general, the problem of antibiotics resistance of causative agents of human diseases including K. pneumoniae continues to intensify. This is a serious threat to world public health that requires action in all sectors of the state.

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