scholarly journals Treatment of cyanide wastes through bioremediation

2016 ◽  
Vol 29 (5) ◽  
pp. 33
Author(s):  
Alma Deloya-Martínez
Keyword(s):  
Biological Treatment ◽  
Manufacturing Industry ◽  
Subsequent Treatment ◽  
Preliminary Treatment ◽  
Gold Mines ◽  
Freeze Dried ◽  
Different Temperatures ◽  
Autochthonous Microorganisms

<p class="p1">This document presents results of research in which an autochthonous consortium of cyanide-degrading microorganisms was developed for use in the biological treatment of hazardous cyanide waste. </p><p class="p1">These autochthonous microorganisms were lyophilized (freeze dried) in different protective media, such as gelatin and lactose broth, at different temperatures (-35,-45,-55 and -65 <span class="s1">o</span>C). </p><p class="p1">The preliminary treatment of cyanide wastes involved pretreatment of sludge for 3-5 days to leach the waste, and a subsequent treatment in aerated lagoons, where the consortium of lyophilized microorganisms was applied. </p><p class="p1">Eight different lyophilized samples were obtained at different temperatures using two protective media for lyophilization, which produced excellent results six months after lyophilization. </p><p class="p1">The consortium of lyophilized microorganisms showed 70% to 80% viability, with cyanide extraction percentages higher than 95%, and can be kept active for long periods of time (for years). </p><p class="p1">Lyophilized microorganisms can be used for biodegradation of cyanide wastes from gold mines or from any other cyanide waste such as that from metallic electroplating baths, or from the jewelry manufacturing industry. </p>

scholarly journals The effect of drying temperature on the properties of gelatin from carps (Cyprinus carpio) skin

2019 ◽  
Vol 37 (No. 4) ◽  
pp. 246-251 ◽  
Author(s):  
Joanna Tkaczewska ◽  
Maciej Wielgosz ◽  
Piotr Kulawik ◽  
Marzena Zajac
Keyword(s):  
Cyprinus Carpio ◽  
Freeze Drying ◽  
Treatment Method ◽  
Thermal Hydrolysis ◽  
Gel Properties ◽  
Drying Temperature ◽  
Freeze Dried ◽  
Different Temperatures ◽  
Pre Treatment ◽  
Hydrolysis Of

The influence of drying temperature on the characteristics and gel properties of gelatine from Cyprinus carpio L. skin was studied. Gelatine was extracted from the carp skin using NaOH and ethanol pre-treatment method, extracted in water in 45°C and then dried in 4 different temperatures: 50, 70, 80°C and freeze-dried. The  electrophoresis and functional properties of gelatines were investigated. Freeze drying allowed to obtain a high gelling force, and all other methods did not give satisfactory results. The proteins in gelatines dried at higher temperatures separated by electrophoresis gave severely blurred bands. It may be explained by thermal hydrolysis of collagen fibrils. Freeze drying is the only effective method for drying this product, which can be used in industry.

scholarly journals Caracterização físico-química e microbiológica do esgoto da UEPB com proposta de tratamento para fins de reuso

2015 ◽  
Vol 10 (3) ◽  
pp. 54
Author(s):  
M. U. Sousa ◽  
L. M. R. Lima
Keyword(s):  
Biological Treatment ◽  
Ammonia Nitrogen ◽  
Preliminary Treatment ◽  
Microbiological Analysis ◽  
State University ◽  
Physical Chemical ◽  
Appropriate Treatment ◽  
Microbiological Characteristics ◽  
Receive Treatment

<p>Nos últimos anos, a preocupação com as questões ambientais vem sendo bastante discutidas, onde merece destaque, o descarte indevido do esgoto doméstico no meio ambiente, portanto o presente estudo objetivou realizar a caracterização físico-química e microbiológica do esgoto descartado no solo, no Centro de Ciências e Tecnologia da Universidade Estadual da Paraíba, campus de Campina Grande – PB. Para tanto foram desenvolvidas análises físico-químicas, tais como pH, Fósforo Total, DBO, DQO, Nitratos, Nitritos, Nitrogênio Amoniacal, Orto-Fosfatos e Alcalinidade, bem como análise microbiológica (coliformes). Observou-se que o efluente na unidade em questão não recebe tratamento para seu descarte, sendo identificadas características físico-químicas e microbiológicas de esgoto <em>in natura</em>. Faz-se necessário desta forma, investimentos em análises periódicas para assim realizar um tratamento adequado ao efluente visando-se reduzir os riscos ambientais e de saúde pública. Após os resultados das análises, foi proposto, além do tratamento preliminar, um tratamento biológico para o efluente, do tipo, lagoa facultativa primária seguida de duas lagoas de maturação, onde a eficiência do tratamento nos parâmetros analisados se enquadra aos padrões estabelecidos pelo CONAMA 430/2011, na qual o efluente pós - tratado poderia ser reutilizado para fins menos nobres, destinando a água potável para atividades que requerem uma qualidade melhor.</p><p><strong><em>Physicochemical and microbiological characterization of wasterwater of the UEPB with proposed of treatment for reuse purposes</em></strong></p><p><strong>Abstract</strong><strong>: </strong>In recent years, concern about environmental issues has been widely discussed, which deserves mention the improper disposal of domestic sewage into the environment, so this study aimed to perform the physicochemical and microbiological sewage discarded the soil, in the Science and Technology Center of the State University of Paraiba, campus of Campina Grande - PB. Therefore, we developed physical-chemical analysis such as pH, Total Phosphorus, BOD, COD, Nitrate, Nitrite, Ammonia Nitrogen, Orto-Phosphates and alkalinity, as well as microbiological analysis (coliforms). It was noted that the effluent in the unit in question does not receive treatment for their disposal, identified physical, chemical and microbiological characteristics of raw sewage. It is necessary therefore, investment in periodic reviews thus to carry out an appropriate treatment to effluent aiming to reduce environmental risks and public health. After the analysis results, it was proposed, in addition to preliminary treatment, a biological treatment for the effluent, type, primary facultative pond followed by two ponds maturation, in which the treatment efficiency in the analyzed parameters falls within the standards set by CONAMA 430/2011 in which the effluent post-treaty could be reused for less noble purposes, intended to clean water for activities that require a better quality<strong>.</strong></p>

scholarly journals P528 Response to biologic therapy in operated Crohn’s disease patients

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S454-S454
Author(s):  
A C Georgieva ◽  
A Atanassova ◽  
M Mirchev
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Clinical Response ◽  
Biological Treatment ◽  
Biological Therapy ◽  
Surgical Intervention ◽  
Mean Value ◽  
Subsequent Treatment ◽  
Time Interval ◽  
Faecal Calprotectin

Abstract Background About 70% of Crohn’s disease (CD) patients undergo surgery due to disease complication. According to the ECCO consensus, in such cases, the tumour necrosis factor-α (TNF-α) antagonists are a means of choice to prevent post-operative relapse. Methods The aim is to evaluate endoscopic, clinical and biochemical outcomes in CD patients, who have undergone surgery, in the course of the subsequent treatment with anti-TNFα mono/combination therapy—anti-TNFα + AZA. Among patients with CD who are undergoing biological treatment, we performed a retrospective analysis of the data of those who underwent surgical intervention associated with Crohn’s disease and subsequently started biological therapy. Results Of the 69 CD patients on biological therapy, surgical intervention was performed in 44.92% (n = 31) of cases prior to the initiation of the treatment. The prevalence of patients with right-sided hemicolectomy with subsequent ileotransverse anastomosis was 54.80%, followed by incision and abscess cavity drainage 22.6%, fistula excision 19.4% and left-sided hemicolectomy 3.2%. In 22 patients (71.0%) there was a clinical response (CDAI decline ≤ 100 points), with 66.66% of them achieving clinical remission (CDAI ≥ 150) (p = 0.001, strong correlation r = 0.596, p = 0.001). During the course of treatment, 23.80% lost clinical response after 18 months of treatment. We compared the mean value of the faecal calprotectin (FCP) before starting, and during the course of the biological treatment, we found that the FCP values decreased 1.5 times: 516.78 ± 273.93, (range 100 – 857)/330.46 ± 432.25, (range 5.32–1800). The activity of the disease measured by CDAI decreases twice during the course of the biological treatment: CDAI 378.00 ± 92.89 (range 258–695)/177.93 ± 116.38 (range 34–414) and CRP values decrease over four times: CRP 59.65 ± 64.52 (range 0.9–225)/13.14 ± 23.59 (range 0.13–116.10). During the course of the biological treatment, intestinal complications were observed in 33.33%, and 9.67% of the patients who had both progression and presence of intestinal complications had a subsequent surgery. In 33.33% of cases, the treatment was intensified. 16.70% of them had to switch to another biological drug. Perianal disease prior to biological treatment in operated patients is twice as common (x2 = 3.82, p = 0.050), which in turn appears to be a risk factor for surgical treatment (OR = 3.47 (0.953–12.685)). Conclusion In the follow up of the relationship between the occurrence of a clinical response and the onset of biological therapy, we found that the time interval was essential. The earlier the anti-TNFα therapy begins, the greater the likelihood of achieving a clinical and biochemical response (r = 0.326, p &lt; 0.05).

scholarly journals Effect of suspending media on freeze-drying and preservation of vaccinia virus

1970 ◽  
Vol 68 (1) ◽  
pp. 29-41 ◽  
Author(s):  
Masatoshi Suzuki
Keyword(s):  
Protective Effect ◽  
Vaccinia Virus ◽  
Carboxymethyl Cellulose ◽  
Freeze Drying ◽  
Chorioallantoic Membrane ◽  
Soluble Starch ◽  
Sodium Glutamate ◽  
Freeze Dried ◽  
Different Temperatures ◽  
Subsequent Storage

SummaryUnpurified and purified smallpox vaccines were prepared from calf dermal pulp, or chorioallantoic membrane (CAM) of hen eggs infected with vaccinia virus, and freeze-dried. The protective effect of various suspending media was investigated both in the course of the freeze-drying and in the period of subsequent storage of the dried product at different temperatures, including 100° C.Single media consisting of either sodium glutamate or peptone were effective in the preservation of both unpurified and purified vaccines prepared from calf dermal pulp or CAM. It was shown that there was an optimal concentration of sodium glutamate for the preservation of the vaccine preparations, especially of the purified vaccine.Combined media, consisting of soluble starch, polyvinylpyrrolidone or sodium carboxymethyl cellulose with sodium glutamate, were effective with the purified vaccine when the concentration of sodium glutamate exceeded the optimum necessary for preservation.

scholarly journals Viability of L. casei during fermentation in soymilk and freeze-dried soymilk; effect of cryoprotectant, rehydration and storage temperature

2006 ◽  
Vol 52 ◽  
pp. 17-24
Author(s):  
Lela Acevska ◽  
Kristina Mladenovska ◽  
Tanja Petreska Ivanovska ◽  
Maja Jurhar Pavlova ◽  
Milena Petrovska ◽  
...  
Keyword(s):  
Freeze Drying ◽  
Storage Temperature ◽  
Acid Stress ◽  
Titratable Acidity ◽  
Therapeutic Level ◽  
Freeze Dried ◽  
Different Temperatures ◽  
And Storage

Viability of L. casei during fermentation in soymilk and freeze-dried soymilk; effect of cryoprotectant, rehydration and storage temperature The aim of the work was to investigate the behaviour of L. casei and the effect of sorbitol on its viability during fermentation in soymilk drink. Values for pH, ranging from 6.82 to 3.42 in the soymilk drink without sorbitol and from 6.74 to 3.41 in the drink with sorbitol were noted during 72 h of fermentation at 25oC. The corresponding values for titratable acidity ranged from 0.071% to 0.758% and from 0.073% to 0.761%, respectively. Soymilk was found to support the growth of L. casei with improvement in viability for 0.24 log at the end of fermentation when sorbitol was added. Survival of L. casei and the effectiveness of sorbitol in improving viability during freeze-drying, subsequent rehydration and during a 5-week period of storage under different temperatures were also investigated. After freeze-drying, L. casei exhibited a survival percent of approximately 46%. Sorbitol improved the viability of L. casei by 0.51 log immediately after freeze-drying and by 1.30 log and 0.47 log during five weeks of storage at 25oC and 4oC, respectively. Further study revealed that the freeze-dried fermented soymilk rehydrated at 45oC was optimum for the recovery of L. casei with improvement in recovery for 0.68 log when sorbitol was added. A higher percent of survival was noted when the dried soymilk was stored at 4oC than at 25oC with improved viability at the end of 5 weeks storage for approximately 6 log for drinks with and without sorbitol. Fermented dried soymilk with sorbitol afforded significant tolerance of L. casei to acid stress. Generally, a stable probiotic diary product was prepared in which the concentration of L. casei remained above therapeutic level of 107 cfu/ml.

Removal of Dyes from the Effluent of Textile and Dyestuff Manufacturing Industry: A Review of Emerging Techniques With Reference to Biological Treatment

2005 ◽  
Vol 35 (3) ◽  
pp. 219-238 ◽  
Author(s):  
HARPREET SINGH RAI ◽  
MANI SHANKAR BHATTACHARYYA ◽  
JAGDEEP SINGH ◽  
T. K. BANSAL ◽  
PURVA VATS ◽  
...  
Keyword(s):  
Biological Treatment ◽  
Manufacturing Industry

scholarly journals Biological Treatment of Wastewater from Pyrolysis Plant: Effect of Organics Concentration, pH and Temperature

Water ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 336
Author(s):  
Luca Di Palma ◽  
Irene Bavasso ◽  
Mauro Capocelli ◽  
Paolo De Filippis ◽  
Vincenzo Piemonte
Keyword(s):  
Organic Carbon ◽  
Total Organic Carbon ◽  
Biological Treatment ◽  
Order Kinetic ◽  
Batch Mode ◽  
Mean Values ◽  
Wood Pyrolysis ◽  
Toc Removal ◽  
Order Kinetic Model ◽  
Different Temperatures

The biological treatment of the aqueous residue produced during poplar wood pyrolysis was investigated. The biological treatment experiments were carried out at two different pH conditions (controlled at 7, uncontrolled) in batch mode at three different temperatures (15 °C, 25 °C and 30 °C) and initial total organic carbon of the water ranging from 800 mg/L to 2800 mg/L. Results show that a substantial removal of organic carbon could be achieved in aerobic conditions after biomass acclimation. After 72 h of treatment, total organic carbon (TOC) removal mean values of 49.47% and 53.03% were observed at 30 °C for solution at 1400 and 2000 mg/L initial TOC, respectively. In the case of 1400 mg/L, a further mineralization (61.80%) was achieved during 144 h of treatment, by using a two-step process. A kinetic study of the process was also made, showing that organics mineralization followed a first-order kinetic model.

scholarly journals Shelf-Life Evaluation and Lyophilization of PBCA-Based Polymeric Microbubbles

Pharmaceutics ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 433 ◽  
Author(s):  
Tarun Ojha ◽  
Vertika Pathak ◽  
Natascha Drude ◽  
Marek Weiler ◽  
Dirk Rommel ◽  
...  
Keyword(s):  
Shelf Life ◽  
Rhodamine B ◽  
Freeze Drying ◽  
Pharmaceutical Products ◽  
Acoustic Properties ◽  
Signal Generation ◽  
Freeze Dried ◽  
Different Temperatures ◽  
The Stability ◽  
Commercial Platform

Poly(n-butyl cyanoacrylate) microbubbles (PBCA-MB) are extensively employed for functional and molecular ultrasound (US) imaging, as well as for US-mediated drug delivery. To facilitate the use of PBCA-MB as a commercial platform for biomedical applications, it is important to systematically study and improve their stability and shelf-life. In this context, lyophilization (freeze drying) is widely used to increase shelf-life and promote product development. Here, we set out to analyze the stability of standard and rhodamine-B loaded PBCA-MB at three different temperatures (4 °C, 25 °C, and 37 °C), for a period of time of up to 20 weeks. In addition, using sucrose, glucose, polyvinylpyrrolidone (PVP), and polyethylene glycol (PEG) as cryoprotectants, we investigated if PBCA-MB can be lyophilized without affecting their size, concentration, US signal generation properties, and dye retention. Stability assessment showed that PBCA-MB remain largely intact for three and four weeks at 4 °C and 25 °C, respectively, while they disintegrate within one to two weeks at 37 °C, thereby compromising their acoustic properties. Lyophilization analyses demonstrated that PBCA-MB can be efficiently freeze-dried with 5% sucrose and 5% PVP, without changing their size, concentration, and US signal generation properties. Experiments involving rhodamine-B loaded MB indicated that significant dye leakage from the polymeric shell takes place within two to four weeks in case of non-lyophilized PBCA-MB. Lyophilization of rhodamine-loaded PBCA-MB with sucrose and PVP showed that the presence of the dye does not affect the efficiency of freeze-drying, and that the dye is efficiently retained upon MB lyophilization. These findings contribute to the development of PBCA-MB as pharmaceutical products for preclinical and clinical applications.

scholarly journals Amino ketone synthesis in avian erythrocytes

1969 ◽  
Vol 114 (3) ◽  
pp. 499-507 ◽  
Author(s):  
R. A. Dale
Keyword(s):  
Pyridoxal Phosphate ◽  
Tris Buffer ◽  
Amino Ketone ◽  
Ph Values ◽  
Freeze Dried ◽  
Avian Erythrocytes ◽  
Different Temperatures ◽  
Metabolic Significance

1. The relative rates of synthesis of aminolaevulate and aminoacetone by particles prepared from avian erythrocytes were measured under various conditions. 2. The production of both amino ketones by fresh particles was about three times greater in anaemia caused by phenylhydrazine and acetylphenylhydrazine than in anaemia caused by removal of 20–30ml. of blood. 3. The synthesis of aminolaevulate by freeze-dried particles decreased more than that of aminoacetone in the absence of added pyridoxal phosphate, in the presence of cyanide and of tris buffer, and after preincubation of the erythrocyte particles. Other differences in the rates of synthesis of the two amino ketones were observed after (a) incubation of particles at different temperatures and (b) storage of homogenized freeze-dried particles at different pH values. 4. It is suggested that these differences in the production of the two amino ketones are due to the presence of two amino ketone synthetases or to two or more isoenzymes of aminolaevulate synthetase. 5. The metabolic significance of aminoacetone in erythrocytes is discussed.

scholarly journals Enhancement of Biogas Production from Rice Husk by NaOH and Enzyme Pretreatment

2018 ◽  
Vol 31 ◽  
pp. 02002 ◽  
Author(s):  
Syafrudin ◽  
Winardi Dwi Nugraha ◽  
Shandy Sarima Agnesia ◽  
Hashfi Hawali Abdul Matin ◽  
Budiyono
Keyword(s):  
Energy Source ◽  
Rice Husk ◽  
Biological Treatment ◽  
Fossil Fuel ◽  
Biogas Production ◽  
Preliminary Treatment ◽  
Enzyme Pretreatment ◽  
Naoh Pretreatment ◽  
Oil And Natural Gas ◽  
The Impact

Biogas is a renewable energy source that can be used as an alternative fuel to replace fossil fuel such as oil and natural gas. This research aims to analyze the impact of NaOH (Sodium hydroxide) and enzyme usage on the production of rice husk biogas using Solid State Anaerobic Digestion (SS-AD). Generally, SS-AD occurs at solid concentrations higher than 15%. The waste of rice husk are used as substrate with a C/N ratio of 25% and the total of solid that are used is 21%. Rice husk contains high lignin, therefore it is handled with chemical and biological treatment. The chemical preliminary treatment was using NaOH with various concentrations from 3%, 6% and 9% while the biological preliminary treatment was using enzyme with various concentration from 5%, 8%, and 11%. The biogas that is produced then measured every two days during 60 days of research with the biogas volume as a parameter observed. The result of the research shows that preliminary treatment with NaOH and enzyme can increase the production of biogas. The highest biogas production was obtained by the NaOH pretreatment using 6% NaOH which was 497 ml and by enzyme pretreatment using 11% enzyme which was 667,5 ml.

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