scholarly journals INFLUENCE OF PEPTIDE IMITATING α-SPIRAL OF B ERYTHROPOETHIN ON THE DEVELOPMENT OF MORPHOFUNCTIONAL DISORDERS IN EXPERIMENTAL PRECLAMPPS

2020 ◽  
Vol 74 (2) ◽  
pp. 53-56
Author(s):  
I.V. Golubev ◽  
◽  
M.A. Zatolokina ◽  
V.V. Gureev ◽  
A.V. Polyanskaya ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Wistar Rats ◽  
New Drugs ◽  
Pathological Changes ◽  
Functional Tests ◽  
Treatment And Prevention ◽  
Urgent Task ◽  
Morphological Picture ◽  
Female Wistar Rats ◽  
Α Helix

Preeclampsia remains a formidable disease in the second half of pregnancy. Therefore, the search for new drugs for the treatment and prevention of preeclampsia is an urgent task of modern pharmacology. Materials and methods. The experiment was performed on female Wistar rats. The studied drugs were administered from 10 to 21 days of pregnancy. Functional tests were performed on the 21st day of pregnancy. Results. The use of a peptide that imitates erythropoietin α-helix B in experimental preeclampsia leads to a pronounced correction of pathological changes: blood pressure decreased, microcirculation in the placenta increased, and the morphological picture in the placenta improved. Resume. The results indicate the promise of the use of a peptide imitating α-helix B erythropoietin in experimental preeclampsia.

scholarly journals CORRECTION OF MORPHOFUNCTIONAL DISORDERS IN EXPERIMENTAL PREECLAMPSY BY COMBINED USE OF TRIMETAZIDINE AND PURIFIED MICRONIZED FLAVONOID FRACTION AS WELL AS THEIR COMBINATIONS WITH METHYLAMPSY

2021 ◽  
Vol 8 (5) ◽  
pp. 304-315
Author(s):  
O. E. Antsiferova ◽  
M. P. Teleshchenko ◽  
Yu. M. Tsuverkalova ◽  
M. V. Pokrovsky ◽  
V. V. Gureev ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Endothelial Dysfunction ◽  
Research University ◽  
New Drugs ◽  
Greater Omentum ◽  
Functional Disorders ◽  
Urgent Task ◽  
Combined Use ◽  
Female Wistar Rats ◽  
Flavonoid Fraction

The aim of the experiment was to determine the effectiveness of the combined use of trimetazidine and a purified micronized flavonoid fraction, as well as their combinations with methyldopa, in comparison with monotherapy with the same drugs in the correction of morphofunctional disorders arising in the conditions of experimental preeclampsia. An integrated/multimethodology approach is the most effective way of treatment for preeclampsia. Therefore, an urgent task of modern pharmacology is to study the effectiveness of new drugs when used in combinations, as well as the drugs included in the standards for treatment.Materials and methods. The study was carried out at the Research Institute of Pharmacology of Living Systems of Belgorod State National Research University. The experiment was performed on 200 female Wistar rats, weighing 250–300 g, in which an ADMA-like model of preeclampsia had been reproduced. To assess the degree of correction of emerging morphological and functional disorders, the following parameters were involved: blood pressure, a coefficient of endothelial dysfunction, microcirculation in the placenta, proteinuria, fluid contents in the greater omentum, morphometric indicators of placental tissues and fetal height and weight parameters.Results. The combined use of trimetazidine (Preductal® MB) 6 mg/kg and a purified micronized flavonoid fraction (Detralex®) 260 mg/kg, as well as their combination with methyldopa (Dopegit®) 86 mg/kg, leads to a more pronounced decrease in the blood pressure, compared with a decrease in the coefficient of endothelial dysfunction by 2.22, 2.19 and 1.94 times, respectively, in relation to “untreated” animals. There was an increase in microcirculation indices in the placenta by 2.35, 2.21 and 2.03 times, respectively. In addition, there was an improvement in morphological parameters in the placenta and fetuses.Conclusion. The results of the study showed a greater effectiveness of the combined use of the studied drugs in experimental preeclampsia compared to their monotherapy. This indicates the prospects for the use of trimetazidine and purified micronized flavonoid fraction in the complex therapy for preeclampsia and the need for further research in this direction.

The Influence of Neonatal Treatment with Guanethidine on the Development of Isolation-Induced Hypertension in Adult Rats

1983 ◽  
Vol 65 (5) ◽  
pp. 469-474 ◽  
Author(s):  
S. M. Gardiner ◽  
T. Bennett
Keyword(s):  
Blood Pressure ◽  
Nervous System ◽  
Sympathetic Nervous System ◽  
Wistar Rats ◽  
Adult Rats ◽  
Male And Female ◽  
Neonatal Treatment ◽  
Induced Hypertension ◽  
Female Wistar Rats ◽  
Sympathetic Nervous

1. Rats housed individually in glass metabolism cages develop hypertension. Since previous experiments have provided some evidence for the involvement of the sympathetic nervous system in the maintenance of the hypertension, the present work was designed to explore the possible involvement of the sympathetic nervous system in the genesis of isolation-induced hypertension. 2. Male and female Wistar rats were treated neonatally with guanethidine, with a protocol designed to produce an extensive peripheral sympathectomy; control rats received saline. 3. The effects of isolation on systolic blood pressure and fluid and electrolyte balances were studied when the rats were mature. 4. Guanethidine-treated rats did not develop hypertension in response to isolation whereas control rats did. 5. There were no significant differences between the fluid and electrolyte balances of the guanethidine-treated rats compared with controls throughout the period of isolation. 6. It is concluded that a fully functional sympathetic nervous system is required for the development of isolation-induced hypertension, but its involvement is not through a modulation of renal function.

Abstract 220: Exercise Training Attenuates Hypertension and Insulin Resistance in Old Fructose-fed Ovariectomized Rats

Hypertension ◽  
2013 ◽  
Vol 62 (suppl_1) ◽  
Author(s):  
Jacqueline F Machi ◽  
Nathalia Bernardes ◽  
Daniele S Dias ◽  
Ivana C Moraes-Silva ◽  
Fernando Dos Santos ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Blood Pressure ◽  
Adipose Tissue ◽  
Exercise Training ◽  
Wistar Rats ◽  
Physical Capacity ◽  
Ovariectomized Rats ◽  
Ovarian Hormone ◽  
Female Wistar Rats ◽  
Hormone Deprivation

Menopause and aging are associated with a gradual increase in systolic blood pressure. Poor eating habits through food containing fructose, have also been related to alterations in the cardiovascular system; however, there are few studies available in the literature relating exercise training (ET), menopause, aging and metabolic syndrome. The aim of the present study was to investigate the effects of ET in ovarian hormone deprivation and/or fructose consumption in old Wistar rats. Female Wistar rats (24 months old) were ovariectomized, fed with fructose (100g/L in drinking water) or normal water, and submitted to an ET protocol in treadmill (1 h/day; 5 days/wk for 8 wk, ~ 40-60% of maximum test) or kept sedentary. The groups were: ovariectomized (O, n= 8), ovariectomized trained (OT, n= 8), ovariectomized fructose (OF, n= 8) and ovariectomized fructose trained (OFT, n= 8). Glycemia, triglycerides, adipose tissue, insulin resistance, physical capacity and blood pressure (BP) were evaluated at the end of the protocol. While the glycemia values remained within the normal range (O:90.1±7.; OT:85.2±2; OF:86±2; OFT:80.6±3 mg/dl) and the triglycerides values were increased in OF group (OF:182±20 vs. O:140.3±9; OT:143.5±7;; OFT:140 ±12 mg/dl). The physical capacity was increased in both OFT (1.8±0.1 Km/h) and OF (1.6±0.07 Km/h) when compared with sedentary peers (O:1.1±0.05; OF: 1.1±0.05 Km/h). The metabolic results showed that ET decreased the adipose tissue in (OT: 6.7±0.9 and OFT: 5.28±0.6 vs. OF: 10.07±0.6 g), and insulin resistance (OT:4.8±0.2 and OFT: 5.0±0.2 vs. OF: 3.2±0.6 mg/dl/%). No differences were observed in O group (O: 6.5±0.8g and 4.5±0.2 mg/dl/min). Finally, ET attenuated the increase in mean BP in both OT and OFT rats (O:119±2; OT 110±2; OF:119±2; OFT: 107±1 mmHg). The same behavior was observed in systolic BP (O:142±2; OT 127±2; OF:143±2; OFT: 129±1 mmHg) and diastolic BP (O:100±2; OT 92±2; OF:98±2; OFT: 89±1 mmHg). In conclusion, low to moderate aerobic ET can attenuate the deleterious effects of ovarian hormone deprivation, and/or fructose consumption, especially the blood pressure increase in old female Wistar rats.

Sex-specific Effects of Spironolactone on Blood Pressure in Gonadectomized Male and Female Wistar Rats

2012 ◽  
Vol 44 (04) ◽  
pp. 291-295 ◽  
Author(s):  
M. Michaelis ◽  
P. Hofmann ◽  
F. Götz ◽  
C. Bartel ◽  
T. Kienitz ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Wistar Rats ◽  
Male And Female ◽  
Specific Effects ◽  
Female Wistar Rats

scholarly journals An experimental study to see the antihypertensive effects of gymnema sylvestre and acorus calamus in wistar rats and its comparison with amlodipine

2017 ◽  
Vol 8 (3) ◽  
pp. 11-15
Author(s):  
Dheeraj Kumar Singh ◽  
Narendra Kumar ◽  
Anjula Sachan ◽  
Preet Lakhani ◽  
Sachin Tutu ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Systolic Blood Pressure ◽  
Wistar Rats ◽  
Oral Feeding ◽  
Acorus Calamus ◽  
Gymnema Sylvestre ◽  
Traditional System ◽  
Female Wistar Rats ◽  
Prevalence Of Obesity ◽  
The Individual

Background: The prevalence of obesity is increasing worldwide. Obesity has a direct correlation with insulin resistance and various cardiovascular diseases like hypertension,dyslipidaemia etc. Hypertension is considered as a major independent risk factor for coronary disease and stroke for all age, race & sex groups. Gymnema sylvestre and Acorus calamus are two well known herbs of traditional system of medicine effective in various diseases.Aims and Objectives: The present study was designed to explore the antihypertensive effects of Gymnema sylvestre (GS) and Acorus calamus (AC), individually as well as in combination, in wistar rats.Materials and Methods: Female wistar rats, weighing between 150- 200 g, were included in the study. They were divided into eight groups with six rats in each group. High Fat diet (HFD) was used to induce hypertension in all the groups except group 1 which was fed with normal rodent diet. Drugs were given to the rats by oral feeding cannula.Results: Feeding rats with HFD for 4 weeks lead to signifi cant increase in the mean systolic blood pressure. Both Gymnema sylvestre and Acorus calamus signifi cantly reduced the systolic blood pressure. Combination of both these (Gymnema sylvestre 200mg/kg and Acorus calamus 200mg/kg) as well as Gymnema sylvestre (200mg/kg) individually has an effect comparable to Amlodipine. Individual drugs had no signifi cant effect on heart rate while the combination of these two drugs signifi cantly reduced the heart rate.Conclusions: The present study concludes that extracts of Gymnema sylvestre and Acorus calamus has a signifi cant antihypertensive action in rodent model of hypertension. Combination is found superior to the individual drugs.Asian Journal of Medical Sciences Vol.8(3) 2017 11-15

Effect of verapamil on myocardial changes in experimental hypertensive rats

1990 ◽  
Vol 48 (3) ◽  
pp. 816-817
Author(s):  
Selma Yilmazer ◽  
Ismail Seçkin ◽  
Mustafa Tasyürekli ◽  
Oktay Arda ◽  
Güngör Satiroglu
Keyword(s):  
Electron Microscopy ◽  
Blood Pressure ◽  
Wistar Rats ◽  
Control Group ◽  
Hypertensive Rats ◽  
Group Iii ◽  
Group I ◽  
Treatment Of Hypertension ◽  
Female Wistar Rats ◽  
Control Group Group

Recent experimental and clinical studies have reported on the effectiveness of calcium antagonists in the treatment of hypertension. The influence of such therapy on the morphologic features of heart, however has not been systematically studied. In the present study we have investigated the effect of verapamil, a calcium antagonist, on blood pressure and on ultrastructural features of myocardium in rats made hypertensive with DOCA + salt.Experiments were carried out with 24 female Wistar rats, 4 weeks of age. The animals were divided into 3, 8 animals per group. Group I was considered as the control group. Group II was injected intraperitoneally with 3 mg/kg daily of DOCA for 5 weeks and 154 NaCl was added in their drinking water. Group III received 8mg/kg daily of verapamil hydrochloride (Isoptin, Knoll Pharmaceutical Co.) orally, in additon to DOCA and salt. Five weeks after the start of the experiment systolic blood pressure was recorded and animals were killed. Left ventricle was dissected and prepared for electron microscopy.

Correction of functional disorders in ADMA-like preeclampsia with derivatives of the peptide imitating erythropoietin α-helix B

2020 ◽  
pp. 42-49
Author(s):  
Vladimir V. Gureev ◽  
Mikhail V. Korokin ◽  
Ivan V. Golubev ◽  
Mikhail V. Pokrovsky ◽  
Adelaida V. Polyanskaya ◽  
...  
Keyword(s):  
Amino Acid ◽  
Wistar Rats ◽  
Diastolic Pressure ◽  
White Female ◽  
Functional Disorders ◽  
Pharmacological Agents ◽  
Amino Acid Peptide ◽  
Female Wistar Rats ◽  
Α Helix ◽  
Derivatives Of

Objective. The aim of the study is to investigate the correction of functional disorders in ADMA-like preeclampsia with derivatives of the peptide imitating erythropoietin α-helix B. Materials and methods. The study was performed in 120 white female Wistar rats weighing 250-300 g. ADMA-like preeclampsia was used as experimental preeclampsia. To assess the effectiveness of the pharmacological agents used, blood pressure, microcirculation in the placenta, and proteinuria were recorded, the content of final NO metabolites in the blood plasma was determined, and the coefficient of endothelial dysfunction was calculated. 50 μg / kg polypeptides (P-αB3 and P-αB4) were used as pharmacological agents, obtained by attaching to the original 11-amino acid peptide PHBSP [PubChem CID: 91810664], which is the amino acid chain Pyr-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser (QEQLERALNSS) of KGD group from different ends of the chain. Results. The use of the studied derivatives of peptide imitating erythropoietin α-helix B with ADMA-like preeclampsia leads to a pronounced correction of disorders. The greatest effect was observed with the introduction of the peptide with the laboratory code P-αB4. A significant decrease in systolic and diastolic pressure was noted, respectively, improved microcirculation in the placenta, restoration of the NO-synthesizing function of the endothelium, and a decrease in proteinuria. Conclusion. The results obtained indicate the prospect of further research on the effectiveness of short-chain derivatives of erythropoietin imitating its α-helix B for the correction of functional disorders in preeclampsia.

scholarly journals Grape Powder Intake Prevents Ovariectomy-Induced Anxiety-Like Behavior, Memory Impairment and High Blood Pressure in Female Wistar Rats

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e74522 ◽  
Author(s):  
Gaurav Patki ◽  
Farida H. Allam ◽  
Fatin Atrooz ◽  
An T. Dao ◽  
Naimesh Solanki ◽  
...  
Keyword(s):  
Blood Pressure ◽  
High Blood Pressure ◽  
Memory Impairment ◽  
Wistar Rats ◽  
Female Wistar Rats

The Effect of Exercise and Protein Source on Food Intake Regulation and Characteristics of Metabolic Syndrome in Obese Female Wistar Rats

2019 ◽  
Vol 15 (2) ◽  
pp. 175-184
Author(s):  
Alireza Jahan-Mihan ◽  
Peter Magyari ◽  
Shawna Jenkins ◽  
Valeria Palamidy ◽  
Lindsay Pappas ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Metabolic Syndrome ◽  
Body Weight ◽  
Food Intake ◽  
Whey Protein ◽  
Soy Protein ◽  
Wistar Rats ◽  
Protein Diet ◽  
Obese Female ◽  
Female Wistar Rats

Background: Beneficial effects of dietary proteins and exercise in treatment of obesity is well-recognized. The effect of exercise and protein source on food intake, body weight and characteristics of metabolic syndrome in obese female Wistar rats was examined. Female Wistar rats received an obesogenic diet for 12 weeks. Then, rats were allocated to four groups and received one of the following treatments for eight weeks: 1- Whey protein Diet + Exercise (WPE), 2- Soy protein diet + exercise (SPE), 3- Whey protein diet, no exercise (WPN), 4- Soy protein diet, no exercise (SPN). The exercise comprised of 30 minutes on a treadmill, three times/week. Body weight (BW) and food intake (FI), blood pressure, pulse, glucose and intake regulatory hormones were measured. Results: FI and plasma ghrelin (2.7 times) were higher in exercise groups compared with non-exercise groups. BW was lower (6.7%) in groups fed a whey protein diet compared with those fed a soy protein diet. Abdominal fat (% BW) was lower (22.8%) in WPE compared with other groups. Diastolic blood pressure (11.1%) and pulse (6%) were lower in groups fed a soy protein diet compared with groups fed a whey protein diet. Conclusion: While exercise affects food intake, source of protein determines BW and BC. Whey protein showed more favorable effect on BW and body composition.

Calcium antagonists preferentially dilate preglomerular vessels of hydronephrotic kidney

1987 ◽  
Vol 253 (6) ◽  
pp. F1157-F1163 ◽  
Author(s):  
J. T. Fleming ◽  
N. Parekh ◽  
M. Steinhausen
Keyword(s):  
Blood Pressure ◽  
Calcium Antagonist ◽  
Wistar Rats ◽  
Afferent Arteriole ◽  
Calcium Blockers ◽  
Efferent Arteriole ◽  
Female Wistar Rats ◽  
Afferent Arterioles ◽  
Arcuate Artery ◽  
Hydronephrotic Kidney

The hydronephrotic kidney of Inactin-anesthetized female Wistar rats was exteriorized in a controlled bath to directly observe preglomerular and postglomerular vessels via television microscopy. Nitrendipine, added to the bath in a concentration that did not alter blood pressure, induced a concentration-dependent dilation of preglomerular vessels. The arcuate artery maximally dilated by 29 +/- 4%, the interlobular artery by 24 +/- 5%, the afferent arteriole near the interlobular artery by 60 +/- 9%, and near the glomerulus by 28 +/- 13%. In contrast the efferent arteriole near the glomerulus dilated by only 11 +/- 6% and near the welling point by 7 +/- 9%. Similarly, diltiazem significantly dilated preglomerular vessels but not efferent arterioles. Acetylcholine significantly dilated all preglomerular vessels and dilated the afferent arterioles near the glomerulus (by 51 +/- 8%) to a greater extent than the calcium blockers. Acetylcholine also significantly dilated the efferent arterioles (near the glomerulus by 26 +/- 5% and near the welling point by 12 +/- 3%). These data suggest that the tone of the preglomerular vessels of the hydronephrotic kidney is more dependent on the entry of extracellular calcium through calcium antagonist-sensitive channels (i.e., potential dependent) than is the tone of the afferent arterioles near the glomerulus and the efferent arterioles.

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