scholarly journals Preliminary Detection of Antibacterial Activity of Fishpond Water Bacteria against Aquaculture Pathogenic Bacteria

Biota ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 91-102
Author(s):  
Stella Magdalena ◽  
Sindy Sindy ◽  
Yogiara Yogiara
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
16S Rrna Gene Sequencing ◽  
Luria Broth ◽  
Rrna Gene ◽  
Clear Zone ◽  
Marine Broth ◽  
Antibacterial Compounds ◽  
Rrna Gene Sequencing ◽  
Potential Use

Aquaculture is currently experiencing massive loss due to the outbreak of pathogenic bacteria. One of the outbreak causes is the development of pathogenic bacterial resistance to the antibacterial. The problem can be solved using microorganisms that can produce new antibacterial compounds. The purpose of this research was to obtain bacteria from fishpond water that could produce antibacterial compounds. About two out of 81 isolates could produce antibacterial compounds. Those two isolates were obtained from saltwater fishponds in North Jakarta (TS2) and Harapan Island (PHY). All fishpond water was grown in marine broth or Luria broth. Extraction of antibacterial compounds was performed using four types of solvents: chloroform, dichloromethane, ethyl acetate, and methanol. Each of the solvents showed a different result. The extraction can only be successfully performed using chloroform and dichloromethane. Extraction using dichloromethane showed a larger inhibitory clear zone than chloroform. Based on 16S rRNA gene sequencing, PHY isolate was identified as Bacillus sp. and TS2 as Acinetobacter sp. In conclusion, isolate TS2 and PHY, which produced antibacterial compounds, showed potential use as aquaculture probiotics.

scholarly journals Identification of Proteus Mirabilis on Banknotes Using 16s rRNA gene in Khartoum State

2018 ◽  
Vol 13 (3) ◽  
pp. 175
Author(s):  
Alaa Abdalla Mukhtar ◽  
Noha Ahmed Abd Alfadil ◽  
Malik Suliman Mohamed ◽  
Hisham N Altayb ◽  
Salaheldein G Elzaki ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Proteus Mirabilis ◽  
Pathogenic Bacteria ◽  
Health Hazard ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Gram Negative Bacteria ◽  
Rrna Gene Sequencing ◽  
Klebsiella Spp

Background The presence of pathogenic bacteria in circulated currency was recorded as a public health hazard. In this study, all examined Sudanese banknotes (100%) were found to be contaminated by gram negative bacteria. Proteus mirabilis were recovered from ten examined notes (22.2%, f=10), E.coli (13.3%, f= 6) and Klebsiella spp. (8.9%, f= 4) were also identified. Only the most resistant Proteus mirabilis isolate was identified using culture-based and 16S rRNA gene sequencing techniques.

scholarly journals 72 PRELIMINARY DATA ON THE PRESENCE OF BACTERIA IN THE UTERUS OF PREGNANT COWS

2016 ◽  
Vol 28 (2) ◽  
pp. 165
Author(s):  
H. G. Pedersen ◽  
L. R. V. Knudsen ◽  
J. S. Agerholm ◽  
T. K. Jensen ◽  
K. S. Klitgaard ◽  
...  
Keyword(s):  
16S Rrna ◽  
Pathogenic Bacteria ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Bacterial Invasion ◽  
Rrna Gene ◽  
Epithelial Surface ◽  
Pregnant Uterus ◽  
Rrna Gene Sequencing

Bacterial invasion of the uterus during the postpartum period has been well described. Recent papers using 16S rRNA gene sequencing techniques suggest that the nonpregnant uterus contains a diverse flora of bacteria that are not necessarily pathogenic. In contrast, the pregnant uterus has until now been considered a sterile environment. The aim of the present study was to investigate whether bacteria were present in the uteri of pregnant cows. Uteri from pregnant, slaughtered animals (n = 47) were sampled. The surface of the uterus was wiped with alcohol, flame sterilized, and cut open with sterile scissors. Samples were taken from the endometrium and from the placentomes. The samples were embedded in paraffin, sectioned at 3 microns, and prepared for fluorescence in situ hybridization using a probe targeting the 16S rRNA of the domain bacteria, so that all bacteria regardless of species were visualised. Using fluorescence microscopy, the presence of bacteria within or on the surface of the endometrium and within the placentomes was noted. The stage of pregnancy was estimated to range from 26 to 263 days by measuring the size of the embryo or fetus. The endometrial samples from 85.1% (40/47) of pregnant cows contained bacteria. In 22 cows, the bacteria were localised within the endometrial tissue, whereas in the remaining 18 cows, the bacteria were on the epithelial surface. Placental samples were obtained from 43 cows, and 76.7% (33/43) of these contained bacteria. The presence of bacteria in the pregnant uterus may suggest that a cow can carry a pregnancy despite the presence of few potentially pathogenic bacteria or that normal flora exist in the uterus as in, for example, the vagina. In conclusion, bacteria were present in the endometrium and placentomes of pregnant cows. Further analyses using rRNA gene sequencing techniques will aim to confirm the presence of bacteria in the bovine pregnant uterus and to investigate which species of bacteria are present in the uterus during pregnancy.

scholarly journals Molecular Identification of Polyhydroxyalkanoates-Producing Bacteria Isolated from Enriched Microbial Community

2013 ◽  
Vol 62 (1) ◽  
pp. 45-50 ◽  
Author(s):  
SŁAWOMIR CIESIELSKI ◽  
TOMASZ POKOJ ◽  
JUSTYNA MOŻEJKO ◽  
EWA KLIMIUK
Keyword(s):  
Bacterial Species ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Bacterial Populations ◽  
Alternative Technologies ◽  
Cultivation Strategy ◽  
Rrna Gene Sequencing ◽  
Promising Source ◽  
Potential Use

Polyhydroxyalkanoates (PHAs) are especially interesting because of their similar properties to synthetic plastics and their potential use as biodegradable polymers. Many strategies have been employed to effectively and economically produce PHAs, among them a production process based on mixed microbial populations, enriched from activated sludge could be one of the alternative technologies. Defining the bacterial species creating these anonymous populations is crucial for the improvement of cultivation strategy. Moreover, enriched bacterial populations could be a promising source for microbes, useful in many biotechnological projects. The main object of this study was to characterize the microorganisms creating the microbial consortium cultured towards PHAs production. After cultivation, bacteria were identified using the 16S rRNA gene sequencing approach. The presence of genes engaged in PHAs synthesis was detected using PCR. The performed analysis revealed that among eleven isolated bacterial strains, four possessed the ability of polyhydroxybutyrate synthesis.

scholarly journals Bacterial isolates from bryozoan Pleurocodonellina sp.: Diversity and antimicrobial potential against pathogenic bacteria

2019 ◽  
Vol 20 (9) ◽  
Author(s):  
Meezan Ardhanu Asagabaldan ◽  
Gilles Bedoux ◽  
Nathalie Bourgougnon ◽  
Rhesi Kristiana ◽  
Diah Ayuningrum ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
16S Rrna Gene Sequencing ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Associated Bacteria ◽  
Antimicrobial Potential ◽  
Rrna Gene Sequencing

Abstract. Asagabaldan MA, Bedoux G, Bourgougnon N, Kristiana R, Ayuningrum D, Sabdono A, Trianto A, Radjasa OK. 2019. Bacterial isolates from bryozoan Pleurocodonellina sp.: Diversity and antimicrobial potential against pathogenic bacteria. Biodiversitas 20: 2528-2535.  There is an urgent need to discover new compounds with antibacterial activity, which can be developed into lead structures for the treatment of human disease caused by multidrug-resistant (MDR) bacteria. In this study, we focussed on bryozoan-associated bacteria to screen them toward antibacterial activities, since the microbiome of these organisms can still be regarded as under-investigated. Most of the few publications about bryozoan-associated bacteria focused on taxonomy and the potential as producers of antibacterial natural products were neglected. Four specimens of bryozoan Pleurocodonellina sp. were collected from Teluk Awur, Jepara in Java Sea, Indonesia. Therefrom, 56 bacterial strains were isolated, and 17 displayed antibacterial activities against MDR bacteria Pseudomonas aruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Enterobacter cloacae, and methicillin-resistant Staphylococcus aureus (MRSA). Taxonomic identification of the bacteria by 16S rRNA gene sequencing revealed them belonging to the genera Virgibacillus, Pseudoalteromonas, Halomonas, and Bacillus. Most interestingly, the genus Virgibacillus was dominantly obtained from the Pleurocodonellina sp. specimens, i.e., 12 active isolates. Nevertheless, the best activities against MDR bacteria (both Gram-positive and Gram-negative) were contributed to isolates showing >99% identity to Pseudoalteromonas. The results further suggest adding the genus Virgibacillus as bacteria associated with bryozoan, since to the best of our knowledge there were no reports of this genus isolated from bryozoan.

scholarly journals Production and Characterization of Keratinolytic Protease from New Wool-DegradingBacillusSpecies Isolated from Egyptian Ecosystem

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Mohamed A. Hassan ◽  
Bakry M. Haroun ◽  
Amro A. Amara ◽  
Ehab A. Serour
Keyword(s):  
Serine Protease ◽  
16S Rrna Gene Sequencing ◽  
Maximum Activity ◽  
Rrna Gene ◽  
Carbon And Nitrogen ◽  
Degrading Bacteria ◽  
Rrna Gene Sequencing ◽  
Potential Use ◽  
Wool Industry

Novel keratin-degrading bacteria were isolated from sand soil samples collected from Minia Governorate, Egypt. In this study, the isolates were identified asBacillus amyloliquefaciensMA20 andBacillus subtilisMA21 based on morphological and biochemical characteristics as well as 16S rRNA gene sequencing.B. amyloliquefaciensMA20 andB. subtilisMA21 produced alkaline keratinolytic serine protease when cultivated in mineral medium containing 1% of wool straight off sheep as sole carbon and nitrogen source. The two strains were observed to degrade wool completely to powder at pH 7 and 37°C within 5 days. Under these conditions the maximum activity of proteases produced byB. amyloliquefaciensMA20 andB. subtilisMA21 was 922 and 814 U/ml, respectively. The proteases exhibited optimum temperature and pH at 60°C and 9, respectively. However, the keratinolytic proteases were stable in broad range of temperature and pH values towards casein Hammerstein. Furthermore the protease inhibitor studies indicated that the produced proteases belong to serine protease because of their sensitivity to PMSF while they were inhibited partially in presence of EDTA. The two proteases are stable in most of the used organic solvents and enhanced by metals suggesting their potential use in biotechnological applications such as wool industry.

scholarly journals Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Dewinta Nur Alvionita ◽  
SRI RAHAYU ◽  
NISA RACHMANIA MUBARIK
Keyword(s):  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Bioactive Compound ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Compound A ◽  
E Coli ◽  
Rrna Gene Sequencing

Hoya multiflora Blume has been used for various purposes. Extract hoya has been reported to have bioactivity but extraction from the plant has been inefficient because it requires large biomass. Therefore, one of the appropriate ways to extract its bioactive compounds is using endophytic bacteria. The purposes of this research were to isolate, characterize, test its antimicrobial activity against two pathogenic bacteria  and identify its bioactive compound. A total of 18 isolates from H. multiflora Blume were successfully obtained. Based on antimicrobial test, one isolate was able to inhibit Escherichia coli, one isolate inhibited Staphylococcus aureus and two isolates inhibited both of pathogenic bacteria. In addition, minimum inhibitory concentration (MIC) of ethyl acetate extract from HMB 1 was 62.5 ppm, while from HMD 4 was 125 ppm, both against E. coli and S. aureus. Based on 16S rRNA gene sequencing analysis, HMB 1 was the most similar (99.91%) with Bacillus siamensis KCTC-13613 and HMD 4 was the most similar (100%) with Bacillus aryabhattai B82W22. Using GC-MS, pyrollo[1,2-a]-pyrazine-1,4-dione was found to be dominant compound in the exctract  bacteria of HMB 1and HMD 4 while Stigmasterol was found in extract HMD 4 and extract leaves H. multiflora Blume, respectively.

scholarly journals A case–control study on the association of intestinal flora with ulcerative colitis

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yin-hua Tang ◽  
Hong-cheng Liu ◽  
Guang Song ◽  
Tian-tian Wu ◽  
Ying Zhao ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Pathogenic Bacteria ◽  
Intestinal Flora ◽  
16S Rrna Gene Sequencing ◽  
Control Group ◽  
Rrna Gene ◽  
Healthy Controls ◽  
Fecal Samples ◽  
Healthy Control ◽  
Rrna Gene Sequencing

AbstractThe association between intestinal flora and ulcerative colitis (UC) was studied in order to provide a basis and method for clinical treatment. Fresh fecal samples were collected from 30 active UC patients and 10 healthy controls. The intestinal flora DNA from each sample was extracted and 16S rRNA gene sequencing was carried out using HiSeq platform to identify the intestinal flora in fecal samples. The richness and diversity of intestinal flora in UC patients were significantly lower than those in healthy control group (P < 0.05). Significant differences were observed between the intestinal flora-species of UC patients and healthy controls. Synergistetes (P < 0.01) and Firmicutes (P < 0.05), along with probiotics Veillonella (P < 0.01), Ruminococcus and Coprococcus (P < 0.05) in the UC patients were lower than that in the healthy controls significantly. Furthermore, compared with the control group, Tenericutes (P < 0.01) and intestinal pathogenic bacteria, including Bacteroides (P < 0.01), Escherichia and Sutterella (P < 0.05) were significantly increased. The incidence of UC is significantly associated with the changes in intestinal flora. Changes in intestinal flora may lead to a decrease in the diversity of intestinal flora or to the enrichment of a particular intestinal flora.

Screening and Molecular Characterization of Cellulase Producing Actinobacteria from Litchi Orchard

2019 ◽  
Vol 13 (1) ◽  
pp. 90-101
Author(s):  
Sanju Kumari ◽  
Utkarshini Sharma ◽  
Rohit Krishna ◽  
Kanak Sinha ◽  
Santosh Kumar
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Biochemical Characterization ◽  
Evolutionary Relationship ◽  
Gene Sequencing ◽  
Cellulase Activity ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Rrna Gene Sequencing

Background: Cellulolysis is of considerable economic importance in laundry detergents, textile and pulp and paper industries and in fermentation of biomass into biofuels. Objective: The aim was to screen cellulase producing actinobacteria from the fruit orchard because of its requirement in several chemical reactions. Methods: Strains of actinobacteria were isolated on Sabouraud’s agar medium. Similarities in cultural and biochemical characterization by growing the strains on ISP medium and dissimilarities among them perpetuated to recognise nine groups of actinobacteria. Cellulase activity was measured by the diameter of clear zone around colonies on CMC agar and the amount of reducing sugar liberated from carboxymethyl cellulose in the supernatant of the CMC broth. Further, 16S rRNA gene sequencing and molecular characterization were placed before NCBI for obtaining recognition with accession numbers. Results: Prominent clear zones on spraying Congo Red were found around the cultures of strains of three groups SK703, SK706, SK708 on CMC agar plates. The enzyme assay for carboxymethylcellulase displayed extra cellulase activity in broth: 0.14, 0.82 and 0.66 &#181;mol mL-1 min-1, respectively at optimum conditions of 35°C, pH 7.3 and 96 h of incubation. However, the specific cellulase activities per 1 mg of protein did not differ that way. It was 1.55, 1.71 and 1.83 μmol mL-1 min-1. The growing mycelia possessed short compact chains of 10-20 conidia on aerial branches. These morphological and biochemical characteristics, followed by their verification by Bergey’s Manual, categorically allowed the strains to be placed under actinobacteria. Further, 16S rRNA gene sequencing, molecular characterization and their evolutionary relationship through phylogenetics also confirmed the putative cellulase producing isolates of SK706 and SK708 subgroups to be the strains of Streptomyces. These strains on getting NCBI recognition were christened as Streptomyces glaucescens strain SK91L (KF527284) and Streptomyces rochei strain SK78L (KF515951), respectively. Conclusion: Conclusive evidence on the basis of different parameters established the presence of cellulase producing actinobacteria in the litchi orchard which can convert cellulose into fermentable sugar.

scholarly journals Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white Pieris canidia (Lepidoptera: Pieridae)

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 254
Author(s):  
Ying Wang ◽  
Jianqing Zhu ◽  
Jie Fang ◽  
Li Shen ◽  
Shuojia Ma ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
16S Rrna Gene Sequencing ◽  
Adult Survival ◽  
Rrna Gene ◽  
Life Stages ◽  
Microbial Composition ◽  
Significant Difference ◽  
Functional Inference ◽  
Rrna Gene Sequencing ◽  
Insect Fitness

We characterized the gut microbial composition and relative abundance of gut bacteria in the larvae and adults of Pieris canidia by 16S rRNA gene sequencing. The gut microbiota structure was similar across the life stages and sexes. The comparative functional analysis on P. canidia bacterial communities with PICRUSt showed the enrichment of several pathways including those for energy metabolism, immune system, digestive system, xenobiotics biodegradation, transport, cell growth and death. The parameters often used as a proxy of insect fitness (development time, pupation rate, emergence rate, adult survival rate and weight of 5th instars larvae) showed a significant difference between treatment group and untreated group and point to potential fitness advantages with the gut microbiomes in P. canidia. These data provide an overall view of the bacterial community across the life stages and sexes in P. canidia.

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