scholarly journals THE PRELIMINARY STUDY OF ANTIOXIDANT ACTIVITY FROM XYLO-OLIGOSACCHARIDE OF CORNCOB (ZEA MAYS) HYDROLYSIS PRODUCT WITH ENDO-β-XYLANASE ENZYME

2016 ◽  
Vol 3 (2) ◽  
pp. 112 ◽  
Author(s):  
Laura Navika Yamani ◽  
Alfinda Novi Kristanti ◽  
Ni Nyoman Puspaningsih
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Hot Spring ◽  
Hydrolysis Product ◽  
Xylanase Enzyme ◽  
Preliminary Study ◽  
Layer Chromatography ◽  
Anti Tumor Activity ◽  
Oligosaccharide Hydrolysis

Xylo-oligosaccharide derived from corncob hemicellulose has been reported to possess antioxidant activity. In order to assess the effective scavenging of xylo-oligosaccharide, we conducted in vitro studies based on self-made xylo-oligosaccharide with DPPH (2,2diphenyl-1-picrilhydrazil) method. Xylo-oligosaccharide was prepared with enzymatic hydrolysis. The enzyme used for hemicellulose hydrolysis was endo-β-xylanase enzyme from PC-01 isolated bactrerium. PC-01 isolated bacterium used in this study was Pacet hot spring which was isolated from East Java. Endo-β-xylanase enzyme is an extracelluler enzyme. There was about 0.199 U/mL after purification and dialysis process. Hydrolisis product of hemicellulose A and B from corncob were analyzed with TLC (Thin Layer Chromatography) and HPLC (High Performance Liquid Chromatography). This analysis showed that hydrolysis product of hemicellulose B had a lot of xylo-oligosaccharide hydrolysis product of hemicellulose than Xylo-oligosaccharide hydrolysis product of hemicelluloses A. Xylo-olygosaccharide was analyzed as on antioxidant activity. Xylo-oligosaccharide hydrolysis product ofhemicellulose B (IC = 48.96) has higher antioxidant activity than Xylo-oligosaccharide hydrolysis product of hemicellulose A (IC 50 50 = 92.302). The toxicity of xylo-oligosaccharide can be calculated by the value of LC 50 (Lethality concentration). LC of xylooligosaccharide derived from corncob hemicellulose was 400 ppm so that xylo-oligosaccharide has anti tumor activity because xylooligosaccharide has LC 50 < 1000 ppm.

HPTLC analysis and in vitro antioxidant activity of aqueous bark extract of Erythrina variegata L.

2016 ◽  
Vol 63 (3) ◽  
pp. 143-157 ◽  
Author(s):  
Kanakasabapathy Devaki
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Erythrina Variegata ◽  
High Antioxidant Activity ◽  
Layer Chromatography

Erythrina variegata L. is an important medicinal plant used in the preparations of Ayurvedic formulations used against several ailments. This study was carried out to investigate the presence of secondary metabolites using phytochemical screening, high-performance thin-layer chromatography (HPTLC) fingerprinting analysis and the antioxidant potential of the aqueous bark extract of E. variegata L. The secondary metabolites and the free radical scavenging activity were analyzed using standard protocols. The results obtained in the present study revealed that E. variegata has high antioxidant activity against free radicals based on phytoconstituents.

A Rapid Quantitative Analysis of Native Antioxidants in Natural Rubber (Hevea brasiliensis) During Maturation

2013 ◽  
Vol 844 ◽  
pp. 410-414
Author(s):  
Natedao Musigamart ◽  
Siriluck Liengprayoon ◽  
Sriroth Klanarong ◽  
Eric Dubreucq ◽  
Jerome Lecomte ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Rubber ◽  
High Performance ◽  
Lipid Analysis ◽  
Oxidative Degradation ◽  
Methodological Development ◽  
Resistance To Oxidation ◽  
Detectable Quantity ◽  
Layer Chromatography

Natural rubber (NR) obtained from H. brasiliensis is known to be susceptible to oxidative degradation according to the amount of double bonds in the structure of the polymer, i.e. poly (cis-1,4-isoprene) [1]. However NR has been reported to contain native antioxidants such as phytosterols, phospholipids and tocotrienols [2]. Among those, γ-tocotrienol, present in hevea latex was reported to exhibit “in-vitro” antioxidant activity [3]. However the direct involvement of γ-tocotrienol or other antioxidants naturally present in NR in the protection of NR against oxidation is difficult to assess as the works were carried out with different of rubber types and in different conditions [4,5]. In the framework of a work on the dynamics of γ-tocotrienols in NR samples obtained from coagula maturated during several durations (0 to 15 days) in parallel with measurement of standard properties such as P0 and PRI, methodological development of the analysis of tocotrienols and derivatives is presented in this study.HPLC-MS has been shown to be an accurate technique for lipid analysis in NR [6]. However, this is a time-consuming technique especially with large number of samples due to a necessary step of saponification. Moreover, the structures of γ-tocotrienol and its dimers were found to be affected by the strong alkali condition of saponification (data not shown). Therefore a rapid quantitative method for γ-tocotrienol from NR using high performance thin layer chromatography (HP-TLC) has been developed. Lipid extracts from NR samples could be simply analyzed by HP-TLC without any derivatization and the detectable quantity could be in nanogram range. Statistical analysis of the data showed that the method is precise, accurate, reproducible and sensitive. Thus the proposed HP-TLC method can be successfully used for the quantification of γ-tocotrienol from NR samples. This technique will be useful to conduct further experiments on antioxidant activity of NR lipids and to relate the results with physical properties of NR, especially those linked to resistance to oxidation.

scholarly journals In Vitro Antiacne and Antidandruff activity of extracted stigmasterol from seed waste of safflower (Carthamus tinctorius L.)

2019 ◽  
Vol 6 (sp1) ◽  
pp. 568-574 ◽  
Author(s):  
Monika Chaudhary ◽  
Vartika Verma ◽  
Nidhi Srivastava
Keyword(s):  
High Performance ◽  
Carthamus Tinctorius ◽  
Diffusion Method ◽  
Safflower Seed ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Preliminary Study ◽  
Waste Extract ◽  
Layer Chromatography

The present study deals with extraction of saponins and their characterization from the seed wastes of safflower. The presence of stigmasterol in the extracted safflower seed waste (S.W.S) was confirmed by Thin Layer Chromatography (TLC), followed by Fourier-Transform Infrared Spectroscopy (FTIR) and High Performance Liquid Chromatography (HPLC) on the basis of its peak compared with stigmasterol standard. FTIR showed the identical functional groups of butanolic extract of SSW with standard while TLC and HPLC showed their notable peak and retention time with the same. Further in-vitro antiacne and antidandruff microbial activity of extracted stigmasterol was confirmed by disc diffusion method. This preliminary study has exhibited anti-acne and anti dandruff potential of Safflower seed waste extract, in future which could be used in therapies and cosmetic applications.

Phytochemical, Analytical and Medicinal Studies of Holoptelea integrifolia Roxb. Planch - A Review

2019 ◽  
Vol 5 (4) ◽  
pp. 270-277 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Ragini Bhadouria ◽  
Ravindra Singh ◽  
Om Prakash
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Toxicological Studies ◽  
Wide Range ◽  
Layer Chromatography

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

scholarly journals In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic Huperzia serrata

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1112
Author(s):  
Yan Yang ◽  
Liangfang Dai ◽  
Decai Wu ◽  
Limin Dong ◽  
Yisheng Tu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
In Vitro Propagation ◽  
Huperzine A ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Regeneration Rate ◽  
Huperzia Serrata ◽  
Chromatography Analysis

Huperzia serrata is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of H. serrata is considered a new way to relieve the resource pressure of H. serrata. In this study, three different genotypic wild H. serrata were used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regenerated H. serrata were investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium for H. serrata was the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L−1 Naphthalene acetic acid (NAA) and 0.1 mg·L−1 2,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L−1), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g−1. The antioxidant experiment showed that the methanol extract of in vitro H. serrata had higher antioxidant activity than that of wild H. serrata. This study provides a reliable in vitro H. serrata culture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.

scholarly journals Delivery of Thyronamines (TAMs) to the Brain: A Preliminary Study

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1616
Author(s):  
Nicoletta di Leo ◽  
Stefania Moscato ◽  
Marco Borso' ◽  
Simona Sestito ◽  
Beatrice Polini ◽  
...  
Keyword(s):  
High Performance ◽  
In Vitro Model ◽  
New Drugs ◽  
Therapeutic Approaches ◽  
Neuroprotective Effects ◽  
Pharmacological Response ◽  
Preliminary Study ◽  
Vitro Model ◽  
The Brain

Recent reports highlighted the significant neuroprotective effects of thyronamines (TAMs), a class of endogenous thyroid hormone derivatives. In particular, 3-iodothyronamine (T1AM) has been shown to play a pleiotropic role in neurodegeneration by modulating energy metabolism and neurological functions in mice. However, the pharmacological response to T1AM might be influenced by tissue metabolism, which is known to convert T1AM into its catabolite 3-iodothyroacetic acid (TA1). Currently, several research groups are investigating the pharmacological effects of T1AM systemic administration in the search of novel therapeutic approaches for the treatment of interlinked pathologies, such as metabolic and neurodegenerative diseases (NDDs). A critical aspect in the development of new drugs for NDDs is to know their distribution in the brain, which is fundamentally related to their ability to cross the blood–brain barrier (BBB). To this end, in the present study we used the immortalized mouse brain endothelial cell line bEnd.3 to develop an in vitro model of BBB and evaluate T1AM and TA1 permeability. Both drugs, administered at 1 µM dose, were assayed by high-performance liquid chromatography coupled to mass spectrometry. Our results indicate that T1AM is able to efficiently cross the BBB, whereas TA1 is almost completely devoid of this property.

scholarly journals Establishment of anIn Vitrod-Cycloserine-Synthesizing System by UsingO-Ureido-l-Serine Synthase and d-Cycloserine Synthetase Found in the Biosynthetic Pathway

2013 ◽  
Vol 57 (6) ◽  
pp. 2603-2612 ◽  
Author(s):  
Narutoshi Uda ◽  
Yasuyuki Matoba ◽  
Takanori Kumagai ◽  
Kosuke Oda ◽  
Masafumi Noda ◽  
...  
Keyword(s):  
Gene Cluster ◽  
High Performance ◽  
Sequence Similarity ◽  
Open Reading Frames ◽  
Homocysteine Thiolactone ◽  
Putative Gene ◽  
Content Type ◽  
Dna Fragment ◽  
Layer Chromatography

ABSTRACTWe have recently cloned a DNA fragment containing a gene cluster that is responsible for the biosynthesis of an antituberculosis antibiotic,d-cycloserine. The gene cluster is composed of 10 open reading frames, designateddcsAtodcsJ. Judging from the sequence similarity between each putative gene product and known proteins, DcsC, which displays high homology to diaminopimelate epimerase, may catalyze the racemization ofO-ureidoserine. DcsD is similar toO-acetylserine sulfhydrylase, which generatesl-cysteine usingO-acetyl-l-serine with sulfide, and therefore, DcsD may be a synthase to generateO-ureido-l-serine usingO-acetyl-l-serine and hydroxyurea. DcsG, which exhibits similarity to a family of enzymes with an ATP-grasp fold, may be an ATP-dependent synthetase convertingO-ureido-d-serine intod-cycloserine. In the present study, to characterize the enzymatic functions of DcsC, DcsD, and DcsG, each protein was overexpressed inEscherichia coliand purified to near homogeneity. The biochemical function of each of the reactions catalyzed by these three proteins was verified by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and, in some cases, mass spectrometry. The results from this study demonstrate that by using a mixture of the three purified enzymes and the two commercially available substratesO-acetyl-l-serine and hydroxyurea, synthesis ofd-cycloserine was successfully attained. Thesein vitrostudies yield the conclusion that DcsD and DcsG are necessary for the syntheses ofO-ureido-l-serine andd-cycloserine, respectively. DcsD was also able to catalyze the synthesis ofl-cysteine when sulfide was added instead of hydroxyurea. Furthermore, the present study shows that DcsG can also form other cyclicd-amino acid analogs, such asd-homocysteine thiolactone.

scholarly journals Application of the thin-layer chromatography method for analysis of plant antioxidant activity

Food systems ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 26-30
Author(s):  
N. V. Kupaeva ◽  
E. R. Vasilevskaya ◽  
L. V. Fedulova ◽  
E. A. Kotenkova
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Chromatography Method ◽  
Physicochemical Methods ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
Active Substances

Plants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by high performance thin layer chromatography (HPTLC) method. The antioxidant activity of the obtained fractions on a chromatographic plate was assessed by subsequent DPPH screening. The extracts red and yellow onion husk and rosemary demonstrated the highest antioxidant activity, variability of the qualitative composition and similarity of antioxidant profiles, while extract of white onion husks did not contain any antioxidant classes. Intensive spots with Rf of 0.13-0.97 were observed along the whole chromatogram track corresponding to red onion husks. It was also found that all tested extract, excepting white onion husk and chaga, contained spots with varying degrees of intensity in the Rf range of 0.96-0.98, which corresponded quercetin Rf value.

scholarly journals HPTLC METHOD FOR ESTIMATION AND QUANTIFICATION OF β-SITOSTEROL FROM IN-VIVO AND IN-VITRO SAMPLES OF MERREMIA AEGYPTIA AND MERREMIA DISSECTA

2020 ◽  
pp. 162-165
Author(s):  
RIDHI JOSHI ◽  
RISHIKESH MEENA ◽  
PREETI MISHRA ◽  
VIDYA PATNI
Keyword(s):  
High Performance ◽  
Normal Phase ◽  
Leaf Sample ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: A normal-phase high-performance thin-layer chromatography (HPTLC) method has been developed and validated for estimation and quantitation of beta-sitosterol from the methanolic fraction of different plant parts of two medicinally important plants viz. Merremia aegyptia and Merremia dissecta. These plants have been reported to possess antimicrobial, antioxidant, and anti-inflammatory activities. Methods: Chromatographic separation of beta-sitosterol from the methanolic extracts of plant parts of M. aegyptia and M. dissecta was performed on TLC aluminum plates pre-coated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at ????-580 nm for ????-sitosterol. Result: Only M. dissecta leaf sample was reported to contain ????-sitosterol (4.6 ng/μl), whereas other samples such as seed, stem, and callus extracts of M. aegyptia and M. dissecta did not showed its presence. Conclusion: The developed HPTLC method is simple, rapid, and precise and can be used for routine analysis and quantification of ????-sitosterol and other useful plant bioactives that are phytopharmaceutically important.

scholarly journals Bioactive Compounds and Antioxidant Activity of Mango Peel Liqueurs (Mangifera indica L.) Produced by Different Methods of Maceration

Antioxidants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 102 ◽  
Author(s):  
Emanuela Monteiro Coelho ◽  
Marcelo Eduardo Alves Olinda de Souza ◽  
Luiz Claudio Corrêa ◽  
Arão Cardoso Viana ◽  
Luciana Cavalcanti de Azevêdo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
High Performance ◽  
Mangifera Indica ◽  
Epigallocatechin Gallate ◽  
Reversed Phase ◽  
Epicatechin Gallate ◽  
Bioactive Content ◽  
Mango Peels

The present work had the objective of producing liqueurs from mango peels (varieties “Haden” and “Tommy Atkins”) by processes of alcoholic maceration and maceration with pectinase, as well as to evaluate bioactive compounds by reversed-phase high-performance liquid chromatography coupled to diode array detection and fluorescence-detection (RP-HPLC/DAD/FD) and in vitro antioxidant activity (AOX), for by-product potential reuse. Alcoholic maceration in wine ethanol (65% v/v) produced liqueurs with higher phytochemical and AOX content. Maceration with pectinase resulted in liqueurs with higher quercetin-3-O-glucopyranoside content. In relation to mango varieties, Haden liqueurs presented higher bioactive content than Tommy Atkins liqueurs. The liqueurs presented high antioxidant activity. The main bioactive compounds found were flavanols (epicatechin-gallate, epigallocatechin-gallate), flavonols (quercetin-3-O-glucopyranoside and rutin), and phenolic acids (gallic acid, o-coumaric acid, and syringic acid). The present study showed that the production of liqueur enabled the recovering of an important part of the bioactive content of mango peels, suggesting an alternative for the recovery of antioxidant substances from this by-product.

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