scholarly journals The correlation between the use of personal protective equipment and level wild-type p53 of dental technicians in Surabaya

2017 ◽  
Vol 50 (1) ◽  
pp. 19 ◽  
Author(s):  
Puspa Dila Rohmaniar ◽  
Titiek Berniyanti ◽  
Retno Pudji Rahayu
Keyword(s):  
Personal Protective Equipment ◽  
P53 Protein ◽  
Pearson Correlation ◽  
P53 Gene ◽  
Working Environment ◽  
Protective Equipment ◽  
Wild Type ◽  
Cross Sectional ◽  
Protein Levels ◽  
Wild Type P53

Background: Exposure of metals among dental technicians that come from the working environment can lead to the formation reactive oxygen species (ROS). ROS can cause mutations in the p53 gene (p53). The mutation is transversion mutation GuanineThymine. p53 mutations can lead to low expression of the wild-type p53 protein (p53). Wild-type p53 involved in many biological processes such as regulation of genes involved in cell cycle, cell growth after DNA damage, and apoptosis. However, exposure to metals among dental technicians can be prevented through the use of personal protective equipment (PPE) during work. Purpose: The purpose of this study was to analyze the correlation between the use of personal protective equipment to wild-type p53 protein levels among dental technicians in Surabaya. Method: This study was observational analytic with cross sectional approach. 40 samples were taken by random sampling. Data were retrieved through interviews and observations. Wild-type p53 was analyzed from saliva with indirect ELISA method. Analysis of data used Kolmogorov Smirnov normality test and a Pearson correlation test. Value significance was p<0.05 (95% confidence level). Result: There was a significant association between the use of personal protective equipment with wild-type p53 levels with p=0.002 Conclusion: The use PPE properly is positively correlated with the wild-type p53 protein levels of dental technicians in Surabaya.

scholarly journals Tumor Eradication by Wild-type p53-specific Cytotoxic T Lymphocytes

1997 ◽  
Vol 186 (5) ◽  
pp. 695-704 ◽  
Author(s):  
Michel P.M. Vierboom ◽  
Hans W. Nijman ◽  
Rienk Offringa ◽  
Ellen I.H. van der Voort ◽  
Thorbald van Hall ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Tumor Cells ◽  
Cytotoxic T Lymphocytes ◽  
Normal Tissue ◽  
P53 Protein ◽  
P53 Gene ◽  
Wild Type ◽  
Wild Type P53 ◽  
Tumor Outgrowth ◽  
Tumor Eradication

The tumor suppressor protein p53 is overexpressed in close to 50% of all human malignancies. The p53 protein is therefore an attractive target for immunotherapy. Cytotoxic T lymphocytes (CTLs) recognizing a murine wild-type p53 peptide, presented by the major histocompatibility complex class I molecule H-2Kb, were generated by immunizing p53 gene deficient (p53 −/−) C57BL/6 mice with syngeneic p53-overexpressing tumor cells. Adoptive transfer of these CTLs into tumor-bearing p53 +/+ nude mice caused complete and permanent tumor eradication. Importantly, this occurred in the absence of any demonstrable damage to normal tissue. When transferred into p53 +/+ immunocompetent C57BL/6 mice, the CTLs persisted for weeks in the absence of immunopathology and were capable of preventing tumor outgrowth. Wild-type p53-specific CTLs can apparently discriminate between p53-overexpressing tumor cells and normal tissue, indicating that widely expressed autologous molecules such as p53 can serve as a target for CTL-mediated immunotherapy of tumors.

scholarly journals Occupational Health Problems, Workplace Environment and Utilization of Personal Protective Equipment among Welders of Banepa Municipality, Nepal

2021 ◽  
Vol 10 (2) ◽  
pp. 100-107
Author(s):  
Manisha Joshi ◽  
Gita Dhakal Chalise ◽  
Sarala Shrestha
Keyword(s):  
Occupational Health ◽  
Personal Protective Equipment ◽  
Structured Interview ◽  
Health Problems ◽  
Vital Role ◽  
Working Environment ◽  
Protective Equipment ◽  
Cross Sectional ◽  
Workplace Environment ◽  
Safety Guidelines

Background: Welding is a hazardous occupation where welders expose to a variety of work-related hazards. These hazards might cause occupational health problems. Safe working environment and utilization of personal protective equipment (PPE) play a vital role in preventing problems and promoting their health, safety and wellbeing. Therefore, this study aimed to identify the prevalence of occupational health problems, workplace environment and utilization of personal protective equipment among welders. Methodology: A descriptive cross- sectional study design was used.  Data was collected from 130 welders of 35 metal workshops in Banepa Municipality using semi-structured interview schedule in 2019. An observational checklist was used to collect information on the workplace environment of selected metal workshops. Data was analyzed by using SPSS version 20 and described by using descriptive and inferential statistical methods. Results: The most prevalent problems experienced by welders were accidents and injuries (99.2%) and eye and ear problems (98.4%). The most available PPE in 35 metal workshops were protective goggles (100%) and insulated gloves (71.4%). Similarly, 90.6% welders always used safety goggles, 41% sometimes used facemasks and 65.4% never used helmets while working.  Only 25.6% workshops had first aid kits available with no expired products and 11.4% had kept fire extinguishers in easily accessible locations. Almost all (97.1%) workshops did not have safety guidelines for their workers. Conclusion: Welders who work in metal workshops experience different types of occupational health problems (OHP) and do not use all types of PPE for their protection. Hence, metal workshops should develop safety guidelines for their workers and strictly implement it to prevent OHP.

scholarly journals The isolation of an RNA aptamer targeting to p53 protein with single amino acid mutation

2015 ◽  
Vol 112 (32) ◽  
pp. 10002-10007 ◽  
Author(s):  
Liang Chen ◽  
Farooq Rashid ◽  
Abdullah Shah ◽  
Hassaan M. Awan ◽  
Mingming Wu ◽  
...  
Keyword(s):  
P53 Protein ◽  
P53 Gene ◽  
P53 Mutation ◽  
Single Amino Acid ◽  
Human Lung Cancer ◽  
Rna Aptamer ◽  
Wild Type ◽  
Wild Type P53

p53, known as a tumor suppressor, is a DNA binding protein that regulates cell cycle, activates DNA repair proteins, and triggers apoptosis in multicellular animals. More than 50% of human cancers contain a mutation or deletion of the p53 gene, and p53R175 is one of the hot spots of p53 mutation. Nucleic acid aptamers are short single-stranded oligonucleotides that are able to bind various targets, and they are typically isolated from an experimental procedure called systematic evolution of ligand exponential enrichment (SELEX). Using a previously unidentified strategy of contrast screening with SELEX, we have isolated an RNA aptamer targeting p53R175H. This RNA aptamer (p53R175H-APT) has a significantly stronger affinity to p53R175H than to the wild-type p53 in both in vitro and in vivo assays. p53R175H-APT decreased the growth rate, weakened the migration capability, and triggered apoptosis in human lung cancer cells harboring p53R175H. Further analysis actually indicated that p53R175H-APT might partially rescue or correct the p53R175H to function more like the wild-type p53. In situ injections of p53R175H-APT to the tumor xenografts confirmed the effects of this RNA aptamer on p53R175H mutation in mice.

scholarly journals MDM2 antagonists induce p53-dependent apoptosis in AML: implications for leukemia therapy

Blood ◽  
2005 ◽  
Vol 106 (9) ◽  
pp. 3150-3159 ◽  
Author(s):  
Kensuke Kojima ◽  
Marina Konopleva ◽  
Ismael J. Samudio ◽  
Masato Shikami ◽  
Maria Cabreira-Hansen ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Transcriptional Activation ◽  
P53 Protein ◽  
Negative Regulator ◽  
Wild Type ◽  
Protein Levels ◽  
P53 Signaling ◽  
P53 Activation ◽  
Induction Of Apoptosis ◽  
Wild Type P53

AbstractAlthough TP53 mutations are rare in acute myeloid leukemia (AML), inactivation of wild-type p53 protein frequently occurs through overexpression of its negative regulator MDM2 (murine double minute 2). Recently, small-molecule antagonists of MDM2, Nutlins, have been developed that inhibit the p53-MDM2 interaction and activate p53 signaling. Here, we study the effects of p53 activation by Nutlin-3 in AML cells. Treatment with MDM2 inhibitor triggered several molecular events consistent with induction of apoptosis: loss of mitochondrial membrane potential, caspase activation, phosphatidylserine externalization, and DNA fragmentation. There was a positive correlation in primary AML samples with wild-type p53 between baseline MDM2 protein levels and apoptosis induced by MDM2 inhibition. No induction of apoptosis was observed in AML samples harboring mutant p53. Colony formation of AML progenitors was inhibited in a dose-dependent fashion, whereas normal CD34+ progenitor cells were less affected. Mechanistic studies suggested that Nutlin-induced apoptosis was mediated by both transcriptional activation of proapoptotic Bcl-2 family proteins, and transcription-independent mitochondrial permeabilization resulting from mitochondrial p53 translocation. MDM2 inhibition synergistically enhanced cytotoxicity of cytosine arabinoside and doxorubicin in AML blasts but not in normal hematopoietic progenitor cells. p53 activation by targeting the p53-MDM2 interaction might offer a novel therapeutic strategy for AML that retain wild-type p53.

scholarly journals DNA strand breaks: the DNA template alterations that trigger p53-dependent DNA damage response pathways.

1994 ◽  
Vol 14 (3) ◽  
pp. 1815-1823 ◽  
Author(s):  
W G Nelson ◽  
M B Kastan
Keyword(s):  
Excision Repair ◽  
P53 Protein ◽  
Dna Strand Breaks ◽  
Wild Type ◽  
Strand Breaks ◽  
Protein Levels ◽  
Dna Damaging Agents ◽  
Wild Type P53 ◽  
Dna Strand ◽  
Dna Template

The tumor suppressor protein p53 serves as a critical regulator of a G1 cell cycle checkpoint and of apoptosis following exposure of cells to DNA-damaging agents. The mechanism by which DNA-damaging agents elevate p53 protein levels to trigger G1/S arrest or cell death remains to be elucidated. In fact, whether damage to the DNA template itself participates in transducing the signal leading to p53 induction has not yet been demonstrated. We exposed human cell lines containing wild-type p53 alleles to several different DNA-damaging agents and found that agents which rapidly induce DNA strand breaks, such as ionizing radiation, bleomycin, and DNA topoisomerase-targeted drugs, rapidly triggered p53 protein elevations. In addition, we determined that camptothecin-stimulated trapping of topoisomerase I-DNA complexes was not sufficient to elevate p53 protein levels; rather, replication-associated DNA strand breaks were required. Furthermore, treatment of cells with the antimetabolite N(phosphonoacetyl)-L-aspartate (PALA) did not cause rapid p53 protein increases but resulted in delayed increases in p53 protein levels temporally correlated with the appearance of DNA strand breaks. Finally, we concluded that DNA strand breaks were sufficient for initiating p53-dependent signal transduction after finding that introduction of nucleases into cells by electroporation stimulated rapid p53 protein elevations. While DNA strand breaks appeared to be capable of triggering p53 induction, DNA lesions other than strand breaks did not. Exposure of normal cells and excision repair-deficient xeroderma pigmentosum cells to low doses of UV light, under conditions in which thymine dimers appear but DNA replication-associated strand breaks were prevented, resulted in p53 induction attributable to DNA strand breaks associated with excision repair. Our data indicate that DNA strand breaks are sufficient and probably necessary for p53 induction in cells with wild-type p53 alleles exposed to DNA-damaging agents.

scholarly journals Técnicos de enfermagem: condições laborais e acidentes em hospital escola

2019 ◽  
Vol 13 ◽  
Author(s):  
Katia Maria Rosa Vieira ◽  
Francisco Ubaldo Vieira Junior ◽  
Zélia Zilda Lourenço de Camargo Bittencourt
Keyword(s):  
Working Conditions ◽  
Personal Protective Equipment ◽  
Biological Material ◽  
Cross Sectional Study ◽  
Public Hospitals ◽  
Working Environment ◽  
Protective Equipment ◽  
Work Processes ◽  
Cross Sectional ◽  
Occupational Risks

Objetivo: analisar as condições de trabalho percebidas por técnicos de Enfermagem e sua relação com acidentes envolvendo material biológico. Método: trata-se de um estudo misto, descritivo, transversal, realizado em um hospital público. Elencaram-se 275 profissionais que responderam a um questionário com perguntas relacionadas ao número de acidentes, percepção das condições de trabalho e sugestões para minimizá-los. Detalha-se que a pesquisa foi do tipo probabilística aleatória simples, com distribuição proporcional nas unidades e turnos de trabalho e os resultados apresentados a partir de relatos e tabelas. Resultados: declarou-se, por 210 técnicos, ter sofrido acidente de trabalho. Revela-se que as variáveis ritmo de trabalho estressante, monotonia e influência do trabalho na saúde tiveram significância estatística, e o uso de equipamentos de proteção individual e diminuição da sobrecarga foram as medidas mais indicadas pelos respondentes. Conclusão: indicou-se, pela análise, relação positiva entre sobrecarga de trabalho e acidentes com material biológico. Percebe-se que, apesar de a instituição disponibilizar equipamentos de proteção individual aos trabalhadores, há forte indício do não uso ou uso parcial. Podem-se subsidiar, pelo instrumento utilizado nesta pesquisa, ações gerenciais para readequações dos processos de trabalho. Descritores: Riscos Ocupacionais; Acidentes de Trabalho; Hospitais Públicos; Ambiente de Trabalho; Condições de Trabalho; Enfermagem.   ABSTRACTObjective: to analyze the working conditions perceived by nursing technicians and their relationship with accidents involving biological material. Method: this is a mixed, descriptive, cross-sectional study conducted in a public hospital. 275 professionals answered a questionnaire with questions related to the number of accidents, perception of working conditions and suggestions to minimize them. It is noted that the research was of simple random probability type, with proportional distribution in units and work shifts and the results presented from reports and tables. Results: 210 technicians claimed to have suffered an accident at work. It is revealed that the variables stressful work rhythm, monotony and influence of work on health were statistically significant, and the use of personal protective equipment and decreased overload were the measures most indicated by respondents. Conclusion: the analysis indicated a positive relationship between work overload and accidents with biological material. Although the institution makes personal protective equipment available to workers, there is strong evidence of non-use or partial use. It can be subsidized, by the instrument used in this research, managerial actions to readjust the work processes. Descriptors: Occupational Risks; Occupational Acidentes; Public Hospitals; Workplace; Working Environment; Nursing.  RESUMENObjetivo: analizar las condiciones de trabajo percibidas por los técnicos de Enfermería y su relación con los accidentes que involucran material biológico. Método: este es un estudio mixto, descriptivo, transversal realizado en un hospital público. Fueron listados 275 profesionales respondieron un cuestionario con preguntas relacionadas con el número de accidentes, la percepción de las condiciones de trabajo y sugerencias para minimizarlos. Se observa que la investigación fue de tipo de probabilidad aleatoria simple, con distribución proporcional en unidades y turnos de trabajo y los resultados presentados a partir de relatos y tablas. Resultados: 210 técnicos afirmaron haber sufrido un accidente en el trabajo. Se revela que las variables ritmo de trabajo estresante, monotonía e influencia del trabajo en la salud fueron estadísticamente significativas, y el uso de equipo de protección personal y la disminución de la sobrecarga fueron las medidas más indicadas por los encuestados. Conclusión: el análisis indicó una relación positiva entre sobrecarga de trabajo y accidentes con material biológico. Si bien la institución pone a disposición de los trabajadores equipos de protección personal, existe una fuerte evidencia de falta de uso o uso parcial. Puede ser subsidiado, por el instrumento utilizado en esta investigación, acciones de gestión para reajustes de los procesos de trabajo. Descriptores: Riesgos Laborales; Accidentes de Trabajo; Hospitales Públicos; Ambiente de Trabajo; Condiciones de Trabajo; Enfermería.

scholarly journals Wild-type p53 gene expression induces granulocytic differentiation of HL-60 cells

Blood ◽  
1994 ◽  
Vol 83 (8) ◽  
pp. 2230-2237 ◽  
Author(s):  
S Soddu ◽  
G Blandino ◽  
G Citro ◽  
R Scardigli ◽  
G Piaggio ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cellular Response ◽  
P53 Protein ◽  
Cell Cycle Control ◽  
P53 Gene ◽  
Malignant Cell ◽  
Temperature Sensitive ◽  
Wild Type ◽  
Granulocytic Differentiation ◽  
Wild Type P53

Abstract Overexpression of wild-type p53 gene in malignant cell lines has been shown to inhibit cell proliferation in a number of cases. However, endogenous p53 protein seems to play little role in normal cell-cycle control as suggested by the normal development of p53 null mice, and by the low p53 protein levels expressed in most cell types. Recently, increased expression of endogenous p53 protein has been observed during the cellular response to DNA damage, as well as during differentiation of human hematopoietic cells. To study the role of the p53 gene in hematopoietic differentiation, we introduced the wild-type p53 gene or the temperature-sensitive p53(Val135) mutant into p53-deficient HL-60 promyelocytic leukemia cells. Morphological analysis, flow-cytometric determination of granulocytic or monocytic surface markers, and ability to reduce nitroblue tetrazolium (NBT) demonstrated that expression of exogenous wild-type p53 gene in HL-60 cells induces differentiation through the granulocytic pathway. Proliferation and cell-cycle analysis performed early after expression of wild-type p53 showed that induction of differentiation is not coupled with growth arrest, which suggests that p53 is involved in differentiation independently of its activity on the cell cycle.

scholarly journals Assessment of the Biosafety and Biosecurity in the Reference Veterinary Laboratory of Parakou in Benin

2021 ◽  
Vol 6 (3) ◽  
pp. 146
Author(s):  
Vincent Dossou Sodjinou ◽  
Paul Ahoumènou Ayelo ◽  
Agué Germain Aïndé Achade ◽  
Dissou Affolabi ◽  
Dona Edgard-Marius Ouendo
Keyword(s):  
Personal Protective Equipment ◽  
Group Discussion ◽  
Working Environment ◽  
Protective Equipment ◽  
Emerging Pathogens ◽  
Cross Sectional ◽  
Health Security ◽  
Mapping Tool ◽  
Food And Agriculture ◽  
Veterinary Laboratory

Optimal biosafety and biosecurity are major requirements of global health security. This study assessed the biorisk management in the reference veterinary laboratory of Parakou (Benin). The study was cross-sectional, descriptive, and evaluative. The non-probability sampling method with the reasoned choice was used. The Food and Agriculture Organization laboratory mapping tool-safety was used to collect information from the laboratory team. Group discussion, working environment observation, and document exploitation were the data collection techniques. The biorisk management was rated good if the average indicator of the laboratory reached at least 80%. Otherwise, the biorisk management was rated insufficient. The overall laboratory biosafety and biosecurity score was insufficient (42.4%). Per area, the scores were 26.7% for engineering, 33.3% for administration, 53.8% for personal protective equipment, and 62.3% for the operational. There was no area or category score that reached 80%. Containment, waste disposal, and personal protective equipment disposal were the best performing categories with a score above 60%. The laboratory has no biosafety and accident prevention program. Its premises require renovation. The standard operating procedures for biosafety are not yet finalized, and the training mechanism is not optimal. Therefore, strong advocacy and implementation of a biorisk management improvement plan appear as urgent corrective actions which are required to help the reference veterinary laboratory of Parakou in its task to protect the livestock and, ultimately, the people of Benin from dangerous diseases and emerging pathogens.

scholarly journals Analysis of p53 mutants for transcriptional activity.

1991 ◽  
Vol 11 (12) ◽  
pp. 6067-6074 ◽  
Author(s):  
L Raycroft ◽  
J R Schmidt ◽  
K Yoas ◽  
M M Hao ◽  
G Lozano
Keyword(s):  
Transcription Factor ◽  
Fusion Protein ◽  
Fusion Proteins ◽  
P53 Protein ◽  
P53 Gene ◽  
Temperature Sensitive ◽  
Mutant P53 ◽  
Permissive Temperature ◽  
Wild Type ◽  
Wild Type P53

The wild-type p53 protein functions to suppress transformation, but numerous mutant p53 proteins are transformation competent. To examine the role of p53 as a transcription factor, we made fusion proteins containing human or mouse p53 sequences fused to the DNA binding domain of a known transcription factor, GAL4. Human and mouse wild-type p53/GAL4 specifically transactivated expression of a chloramphenicol acetyltransferase reporter in HeLa, CHO, and NIH 3T3 cells. Several mutant p53 proteins, including a mouse p53 mutant which is temperature sensitive for suppression, were also analyzed. A p53/GAL4 fusion protein with this mutation was also transcriptionally active only at the permissive temperature. Another mutant p53/GAL4 fusion protein analyzed mimics the mutation inherited in Li-Fraumeni patients. This fusion protein was as active as wild-type p53/GAL4 in our assay. Two human p53 mutants that arose from alterations of the p53 gene in colorectal carcinomas were 30- to 40-fold less effective at activating transcription than wild-type p53/GAL4 fusion proteins. Thus, functional wild-type p53/GAL4 fusion proteins activate transcription, while several transformation competent mutants do so poorly or not at all. Only one mutant p53/GAL4 fusion protein remained transcriptionally active.

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