The effect of Avocado leaf extract (Persea americana Mill.) on the fibroblast cells of post-extraction dental sockets in Wistar rats

Background: Tooth extraction, a common practice among the dental profession, causes trauma to the blood vessels during the wound healing process. The acceleration of wound healing, within which fibroblasts play an important role, is influenced by nutrition. Avocado leaves contain a variety of chemicals, including flavonoid compounds, tannins, katekat, kuinon, saponin and steroids/triterpenoid. Avocado leaves also contain glycosides, cyanogenic, alkaloids and phenols which function as anti-inflammatory, antibacterial and antioxidant agents. This avocado leaf content could be used as an alternative medicine to accelerate the wound healing process in post-tooth extraction sockets. Purpose: To determine the role of avocado leaves (Persea americana Mill) in accelerating fibroblast cells proliferation in tooth socket post-extraction. Methods: The sample was divided into four groups, a control group and three treatment groups. The treatment groups used avocado leaf extract and 3% CMC Na solution which was inserted into the tooth sockets of Wistar rats. Both the control and treatment groups had their mandibula decapitated with all the required specimens being prepared on the 3rd and 7th days of the experiment. Mandibular decapitation and tooth extraction socket were prepared by HPA (Histology Pathology Anatomy) with Hematoxylin Eosin (HE) staining. The fibroblast proliferation was analyzed by means of a light microscope at 400x magnification. The obtained data was analyzed using a t-Test. Result: The t-Test obtained a significance value 0.001 (p <0.05) between the control and treatment groups. The number of fibroblast cells increased in the group treated on the third day and decreased in the group treated on the seventh day. Conclusion: Avocado leaf extract (Persea americana Mill.) accelerates proliferation of fibroblast cells in Wistar rats post-tooth extraction.

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Expression of fibroblast cells after extraction of wistar rat teeth after topical application of okra fruit (Abelmoschus esculentus) gel

Infectious Disease Reports ◽

10.4081/idr.2020.8726 ◽

2020 ◽

Author(s):

Muhammad Luthfi ◽

Wisnu Setyari Juliastuti ◽

Yuniar Aliyah Risky ◽

Elvina Hasna Wijayanti ◽

Aisyah Ekasari Rachmawati ◽

...

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Wistar Rats ◽

Tooth Extraction ◽

Healing Process ◽

Wistar Rat ◽

Control Group ◽

Wound Healing Process ◽

Fibroblast Cells ◽

Fruit Extract

Background: Tooth extraction is a dental procedure for removing a teeth from the alveolar bone socket. The tooth extraction process causes damage to hard tissue and soft tissue, so the body will respond physiologically to heal the wound. The wound healing process is divided into several phases, one of which is the proliferation phase of fibroblasts, which is one of the most important phases in the process of wound healing. Okra fruit contains saponins, tannins, flavonoids and alkaloids that have anti-inflammatory, antibacterial, antioxidant effects, and can stimulate angiogenesis so to accelerate the process of wound healing. Objective: to prove that the administration of okra fruit extract can accelerate the process of wound healing after extraction in the teeth of Wistar rats through increased expression of fibroblast cells. Methods: 18 Wistar rats were divided into 2 groups; control group and treatment group. The treatment group received a 30% okra fruit extract. The number of fibroblasts was calculated statistically using One Way ANOVA and Tukey HSD. Results: The results showed that the expression of control group fibroblast cells on day 3 (19.00 ± 2.0), day 5 (21.67 ± 2.08), day 7 (24.00 ± 2.00), whereas in the treatment group on day 3 (24.00 ± 1.00), day 5 (29.00± 2.00), day 7 (30.00 ± 1.53). Anova test between groups showed a significant difference with P-value 0.006. Conclusion: 30% okra fruit extract can increase fibroblast expression in wound healing process after extraction of Wistar rat teeth.

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Esktrak ikan haruan (Channa striata) menurunkan jumlah makrofag pada fase inflamasi proses penyembuhan luka (Extract of haruan (Channa striata) decreases macrophages count in inflammation phase of wound healing process )

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v14i1.417 ◽

2015 ◽

Vol 14 (1) ◽

pp. 6

Author(s):

Putri Sri Hartini ◽

Nurdiana Dewi ◽

Lisda Hayatie

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Wistar Rats ◽

Healing Process ◽

Positive Control ◽

Wound Healing Process ◽

Treatment Groups ◽

Accelerate Wound Healing ◽

Post Test ◽

Channa Striata

Haruan is a kind of freshwater fishes commonly found in South Kalimantan. Haruan extract contains nutritional substancessuch as albumin, Zn, Fe, Cu, and unsaturated fatty acid which can help accelerate wound healing. The aim of this studywas to histopathologically assess the effect of 100%, 50%, and 25%haruan extract compared to aquadest and ibuprofenon macrophages count in inflammation phase of wistar rats’ buccal mucosa wound healing. This research was a trueexperimental with post test-only with control design. Samples used were 30 wistar rats divided into 5 groups, 100%, 50%,25% haruan extract treatment groups, ibuprofen treatment group as positive control, and aquadest treatment group asnegative control. Mean macrophages counted on day 3 of 100%, 50%, 25% haruan extract treatment groups aquadest,and ibuprofenl were 2.05, 4.4, 3.9, 3.3, 2.4 respectively. In conclusion, haruan extract had a significant effect in decreasingmacrophages count in inflammation phase of wound healing.

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Effect of haruan (Channa striata) extract on fibroblast cells count in wound healing

Journal of Dentomaxillofacial Science ◽

10.22208/jdmfs.1.2.2016.226-231 ◽

2016 ◽

Vol 1 (2) ◽

pp. 226

Author(s):

Ardi Siswanto ◽

Nurdiana Dewi ◽

Lisda Hayatie

Keyword(s):

Wound Healing ◽

Healing Process ◽

Negative Control ◽

Control Group ◽

Wound Healing Process ◽

Fibroblast Cells ◽

Control Group Design ◽

Treatment Groups ◽

Accelerate Wound Healing ◽

Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with<em> aquadest</em> for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p<0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

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FGF-2 expression and the amount of fibroblast in the incised wounds of Rattus norvegicus rats induced with Mauli banana (Musa acuminata) stem extract

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v50.i3.p121-126 ◽

2018 ◽

Vol 50 (3) ◽

pp. 121

Author(s):

Didit Aspriyanto ◽

Intan Nirwana ◽

Hendrik Setia Budi

Keyword(s):

Wound Healing ◽

Rattus Norvegicus ◽

Healing Process ◽

Control Group ◽

Wound Healing Process ◽

Fibroblast Cells ◽

Treatment Groups ◽

Incised Wound ◽

Stem Extract ◽

Banana Stem

Background: Traditional wound treatment using herbal medicine is thought to maintain the health of families and society in general economically, effectively, and efficiently without inducing side effects. One genus of plant that can be used as a traditional medicine is the Mauli banana, indigenous to South Borneo. Mauli banana stem contains bioactive compounds, most of which are tannins along with ascorbic acid, saponin, β-carotene, flavonoids, lycopene, alkaloids, and flavonoids. Tanin has antibacterial and antioxidant effects at low concentrations, as wells as antifungal ones at high concentrations. Purpose: This study aimed to analyze the effects of Mauli banana stem extract at concentrations of 25%, 37.5%, and 50% on the quality of incised wound healing in male Rattus norvegicus rats by assessing FGF-2 expression and fibroblast concentration on days 3 and 7. Methods: This research represented an experimental laboratory-based investigation involving 32 rats of the Rattus norvegicus strain aged 2-2.5 months old. Sampling was performed using a simple random sampling technique since the research population was considered homogeneous and divided into 8 treatment groups (C3, M3-25, M3-37.5, M3-50, C7, M7-25, M7-37.5, M7-50). The rats in each group were anesthetized before their back was incised with length and width of 15x15mm with a depth of 2mm. Gel hydroxy propyl cellulose medium (HPMC) was applied to the incised wound of each rat in the control group, while stem Mauli banana extract was applied to that of each rat in the treatment groups three times a day at an interval of 6-8 hours. On day 3, four rats from each group were sacrificed, while, in the remaining groups, the same procedure was performed until day 7, at which point they (8 groups) were sacrificed for HE examination in order to assess the amount of fibroblast and for IHC examination to examine FGF-2 expression. Data regarding FGF-2 expression and the amount of fibroblast were analysed by means of One-way Anova and HSD. Results: The results showed that the Mauli banana stem extract could significantly improve the expression of FGF-2 and the amount of fibroblast cells compared to C3 and C7 groups. The highest increase in FGF-2 expression and fibroblast amount were found in all groups on days 3 and 7 treated with the Mauli banana stem extract at a concentration of 50%. Conclusion: There was an increase of FGF-2 expression and the amount of fibroblast cells in the incision wound healing process that induced with Mauli banana stem extract.

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Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: in vivo studies in wistar rats

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v49.i3.p125-132 ◽

2016 ◽

Vol 49 (3) ◽

pp. 125

Author(s):

Gunawan Oentaryo ◽

Istiati Istiati ◽

Pratiwi Soesilawati

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Healing Process ◽

Tissue Growth ◽

Control Group ◽

Wound Healing Process ◽

Lower Lip ◽

Treatment Groups ◽

Collagen Secretion ◽

Channa Striata

Background: Wound healing is a biological process associated with tissue growth and regeneration. Wound healing process, is important to repair damaged tissue. Wound healing process consists of coagulation and hemostasis, inflammation, proliferation, as well as remodeling phases. The process can be accelerated by taking synthetic or non synthetic drugs. One of them is Channa striata extract. The extract contains albumin, copper, and zinc, which can be assumed to increase inflammatory cell infiltration, fibroblast proliferation, and collagen secretion. Purpose: This study aimed to reveal the effects of Channa striata extracts on fibroblast number and FGF-2 expression in mucosal wound healing process of the Wistar rats’ lower lip. Method: This research was a true laboratory experimental research with randomized post test only control group design. Samples of experiment were devided to experiment and control group that consist five samples each. Experimental group was treted with Channa striata extract and ethanol at concentration of 25%, 50%, and 100%. The fibroblast number and FGF-2 expresion were examined. Result: The number of fibroblasts in the treatment groups receiving Channa striata extract at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest number of fibroblasts was found on day 3 at the concentration of 100% (p<0.05). Similarly, FGF-2 expression in the treatment groups receiving Channa striata at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest expression of FGF-2 was found on day 3 at the concentration of 50% (p<0.05). Conclusion: Channa striata extract increased fibroblast number and FGF-2 expression in mucosa wound healing process.

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The effect of 3% binahong leaf extract gel on the wound healing process of post tooth extraction

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v54.i2.p57-62 ◽

2021 ◽

Vol 54 (2) ◽

pp. 57

Author(s):

Olivia Avriyanti Hanafiah ◽

Diana Sofia Hanafiah ◽

Rahmi Syaflida

Keyword(s):

Wound Healing ◽

Tooth Extraction ◽

Leaf Extract ◽

Healing Process ◽

Control Group ◽

Wound Healing Process ◽

Herbaceous Plant ◽

Significant Difference ◽

Analyse Data ◽

And Control

Background: One of the common frequently performed procedures in dentistry is tooth extraction. After tooth extraction, a series of biological events occurs in the alveolar socket that ultimately results in healing of the socket. Binahong (Anredera cordifolia) is an herbaceous plant that contains active compounds that may accelerate the wound healing process. Purpose: The aim of this study is to investigate the effect of 3% binahong leaf extract gel on the healing process of the postextraction wound. Methods: 3% binahong leaf extract gel was prepared with the composition of 1 g carbopol, 1 g hydroxypropilmethylcellulose (HPMC), 4 g glycerin, 3 g triethanolamine (TEA), 0.04 g Nipagin, 0.04 g Nipasol, 1.2 g binahong leaf extract and Aq ad 40 g. A total of 18 people were subjected to tooth extraction and were then divided into two groups, with nine people in the binahong group and nine in the control group. Following that, residual socket volume (RSV) was examined on days 3, 7 and 14 after the extraction in both the binahong and control groups. The RSV value was obtained by calculating the mesiodistal x bucolingual width x the depth probing and measured using a calliper and probe. Repeated analysis of variance (ANOVA) and Friedman test followed by Wilcoxon and dependent t-test (p<0.05) were used to analyse data. Results: Significant difference in the mean RSV between the control and binahong groups was observed on days 3, 7 and 14 post tooth extraction. The RSV value in the binahong group was lower when compared to the control group (p <0.05). Conclusion: 3% binahong leaf extract gel can accelerate the process of healing socket wounds.

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Efek Pemberian Ekstrak Lidah Buaya (Aloe Barbadensis Miller) pada Soket Gigi terhadap Kepadatan Serabut Kolagen Pasca Ekstraksi Gigi Marmut (Cavia Porcellus)

Majalah Kedokteran Gigi Indonesia ◽

10.22146/majkedgiind.8743 ◽

2014 ◽

Vol 21 (2) ◽

pp. 127

Author(s):

Fatma Yuza ◽

Ivan Arie Wahyudi ◽

Sri Larnani

Keyword(s):

Wound Healing ◽

Guinea Pig ◽

Tooth Extraction ◽

Healing Process ◽

Collagen Fibers ◽

Control Group ◽

Wound Healing Process ◽

Cavia Porcellus ◽

Aloe Barbadensis ◽

Treatment Groups

Tindakan ekstraksi gigi menyebabkan terjadinya luka sehingga akan melibatkan proses penyembuhan luka pada jaringan. Salah satu tahap penting dari proses penyembuhan luka pasca esktraksi gigi adalah terbentuknya serabut kolagen. Lidah buaya (Aloe barbadensis Miller) mengandung saponin, vitamin C dan acemannan yang diduga membantuproses pembentukan serabut kolagen. Tujuan penelitian ini adalah untuk mengetahui pengaruh ekstrak lidah buaya terhadap kepadatan serabut kolagen pada proses penyembuhan luka pasca ekstraksi gigi marmut (Cavia porcellus). Lidah buaya yang digunakan berasal dari Sleman, Yogyakarta. Pembuatan ekstrak menggunakan metode maserasi dan pelarut air. Selanjutnya, dua puluh tujuh ekor marmut dibagi ke dalam kelompok kontrol dan kelompok perlakuan. Kelompok perlakuan terdiri dari kelompok ekstrak lidah buaya 45% dan 90%. Ekstrak lidah buaya sebanyak 0,05ml diteteskan kedalam soket gigi marmut pasca ekstraksi gigi pada kelompok perlakuan. Soket gigi marmut kelompok kontrol tidak diberi aplikasi zat aktif apapun. Tiga ekor subjek dari masing-masing kelompok dikorbankan pada hari ke-3, 7, dan 14 setelah ekstraksi gigi. Preparat histologis kepadatan kolagen soket gigi marmut diamati dengan menggunakan mikroskop cahaya perbesaran 400x. Analisis data dilakukan dengan menggunakan uji Kruskal-Wallis dan dilanjutkandengan uji Post Hoc menggunakan uji Mann-Whitney untuk membandingkan kepadatan kolagen antar kelompok pasca ekstraksi gigi marmut. Hasil uji statistik antar kelompok menunjukkan bahwa ekstrak lidah buaya 90% berpengaruh pada pembentukan serabut kolagen jika dibandingkan dengan kelompok kontrol (p<0,05) pada hari ke-7 pasca ekstraksi gigi marmut. Kesimpulan dari penelitian ini adalah ekstrak lidah buaya 90% dapat membantu meningkatkan kepadatan serabut kolagen soket gigi hari ke-7 pasca ekstraksi gigi marmut. The Effect of Aloe Barbadensis Miller Extract to The Density of Collagen Fibers in The Wound Healing Process after Tooth Extraction of Guinea Pig (Cavia porcellus). Tooth extraction causes wound that would involve wound healing process on tissue. One of the important stages of wound healing process after dental extraction is the formation of collagen fibers. Aloe barbadensis Miller contains saponins, vitamin C and ace mannan that allegedly assist the process of collagen fibers formation. The purpose of this study was to determine the effect of Aloe barbadensis Miller extract to the density of collagen fibers in the wound healing process after tooth extraction of guinea pig (Caviaporcellus). Aloe vera is used in this study came from Sleman, Yogyakarta. Extract is made with maceration method and water as the solvent. Furthermore, twenty-seven guinea pigs were divided into a control group and treatment groups. The treatment groups consisted 45% Aloe barbadensis Miller extract group and 90% Aloe barbadensis Miller extractgroup. Aloe barbadensis Miller extract as much as 0.05 ml dropped into guinea pigs tooth sockets after tooth extraction in the treatment groups. Guinea pig’s tooth socket of the control group was not given any active substance. Three guinea pigs of each group were sacrificed on day 3, 7, and 14 after tooth extraction. Histology preparations of guinea pig teethsockets density of collagen were observed using light microscope 400x magnification. Analyzing data is done by Kruskal-Wallis test followed by Post Hoc test using the Mann-Whitney test for comparing collagen density between groups. Statistically results between groups showed that the extract of 90% Aloe barbadensis Miller affected the formation of collagen fibers when compared to the control group (p <0.05) on day 7 after tooth extraction of guinea pig. The conclusion of this study was 90% Aloe barbadensis Miller extract increased the density of collagen fibers from the tooth socket seven days after tooth extraction of guinea pig.

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EFEKTIVITAS LENDIR BEKICOT (ACHATINA FULICA) TERHADAP JUMLAH SEL FIBROBLAS PADA LUKA PASCA PENCABUTAN GIGI TIKUS WISTAR

e-GIGI ◽

10.35790/eg.3.2.2015.10325 ◽

2015 ◽

Vol 3 (2) ◽

Author(s):

Christal G. Oroh ◽

Damajanty H. C. Pangemanan ◽

Christy N. Mintjelungan

Keyword(s):

Wound Healing ◽

Serum Protein ◽

Tooth Extraction ◽

Healing Process ◽

T Test ◽

Control Group ◽

Wound Healing Process ◽

Achatina Fulica ◽

Control Group Design ◽

Group Design

Abstract: Tooth extraction is a common procedure in dentistry and can produce an injury. The main cells involved in wound healing are the fibroblasts. Snails are animals that were encountered in Indonesia. Snail slime contains beta agglutinins (antibodies) in the plasma (serum), protein achasin, glikokonjugat and acharan sulphate plays a role in wound healing process by helping the blood clotting process and proliferation of fibroblasts. The purpose of this study was to examine the effectiveness of snail slime on the number of fibroblasts in the wound after tooth extraction Wistar rats. This study is a laboratory experimental design with posttest only control group design using 10 rats Wistar male were divided into 2 groups: the treatment group were extracted incisor left underneath and given the snail slime, and the control group were not given the snail slime after extraction of teeth bottom left incisor. Number of fibroblast cells was observed at day 5 after tooth extraction. Snails were taken from plantations in the area Kalasey. This research was conducted in the Laboratory of Pathology of the Faculty of Medicine, University of Sam Ratulangi. The results showed the average number of fibroblasts in the control group less, with a value of 34.4 compared with the group treated with the value of 70.2. Data from each group were analyzed using normality test, homogeneity and continued Independent t-test. Conclusion: Snail slime was effective to increase the number of fibroblasts after tooth extraction of Wistar rats.Keywords: snail slime (achatina fulica), fibroblasts, tooth extraction, male wistar rats.Abstrak: Pencabutan gigi merupakan prosedur umum dalam kedokteran gigi dan dapat menghasilkan suatu perlukaan. Sel utama yang terlibat dalam proses penyembuhan luka ialah fibroblas. Bekicot merupakan hewan yang banyak ditemui di Indonesia. Lendir Bekicot mengandung zat beta aglutinin (antibodi) di dalam plasma (serum), protein achasin, glikokonjugat dan acharan sulfat yang berperan dalam proses penyembuhan luka dengan membantu proses pembekuan darah dan proliferasi sel fibroblas. Tujuan penelitian ini adalah untuk mengetahui efektivitas lendir bekicot terhadap jumlah sel fibroblas pada luka pasca pencabutan gigi tikus wistar. Penelitian ini merupakan penelitian eksperimental laboratoris dengan desain post test only control group design dengan menggunakan 10 ekor tikus wistar jantan yang dibagi menjadi 2 kelompok yaitu kelompok perlakuan yang diekstrasi gigi insisivus kiri bawahnya dan diberikan lendir bekicot, dan kelompok kontrol yang tidak diberikan lendir bekicot setelah ekstrasi gigi insisivus kiri bawahnya. Jumlah sel fibroblas diamati pada hari ke-5 setelah pencabutan gigi. Bekicot diambil dari perkebunan di daerah Kalasey. Penelitian ini dilakukan di Laboratorium Patologi Anatomi Fakultas Kedokteran Universitas Sam Ratulangi Manado. Hasil penelitian menunjukkan jumlah rata-rata sel 515Oroh, Pangemanan, Mintjelungan: Aktivitas lendir bekicot...fibroblas pada kelompok kontrol lebih sedikit, dengan nilai 34,4 dibandingkan dengan kelompok perlakuan dengan nilai 70,2. Data dari masing-masing kelompok dianalisa menggunakan uji normalitas, uji homogenitas dan dilanjutkan Independent t-test. Hasil penelitian ini menunjukkan bahwa lendir bekicot memiliki efektifitas terhadap peningkatan jumlah sel fibroblas pasca pencabutan gigi tikus wistar.Kata kunci: lendir bekicot (achatina fulica), fibroblas, pencabutan gigi, tikus wistar jantan

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Effect of haruan (channa striata) extract on fibroblast cells count in wound healing

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v1i2.3 ◽

2016 ◽

Vol 1 (2) ◽

pp. 89

Author(s):

Ardi Siswanto ◽

Nurdiana Dewi ◽

Lisda Hayatie

Keyword(s):

Wound Healing ◽

Healing Process ◽

Negative Control ◽

Control Group ◽

Wound Healing Process ◽

Fibroblast Cells ◽

Control Group Design ◽

Treatment Groups ◽

Accelerate Wound Healing ◽

Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with aquadest for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p<0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

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Pengaruh gel ekstrak daun binahong (Anredera cordifolia) 5% terhadap peningkatan osteoblas pada proses penyembuhan luka pasca pencabutan gigi tikus strain WistarEffect of 5% binahong (Anredera cordifolia) leaf extract in increasing the osteoblast amount at the tooth extraction wound healing process of Wistar rat

Jurnal Kedokteran Gigi Universitas Padjadjaran ◽

10.24198/jkg.v32i1.26885 ◽

2020 ◽

Vol 32 (1) ◽

pp. 9

Author(s):

Jannatus Salis Sa'diyah ◽

Dewi Ayu Septiana ◽

Nanda Nailul Farih ◽

Juwita Raditya Ningsih

Keyword(s):

Wound Healing ◽

Tooth Extraction ◽

Leaf Extract ◽

Healing Process ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Wound Healing Process ◽

Control Group Design

Pendahuluan: Pencabutan gigi di dunia kedokteran gigi adalah tindakan yang sering dilakukan. Pasca terjadinya pencabutan gigi, maka tulang pada soket akan rusak. Penyembuhan tulang ditandai dengan terbentuknya sel-sel osteoblas baru. Daun binahong merupakan daun yang mengandung flavonoid, alkaloid, terpenoid, asam askorbat, saponin, tanin dan asam oleanolik. Kandungan dari daun binahong dapat meningkatkan jumlah osteoblas pasca pencabutan gigi. Tujuan dari penelitian adalah menganalisis pengaruh pemberian gel ekstrak daun binahong (Anredera cordifolia) konsentrasi 5% terhadap peningkatan osteoblas pada proses penyembuhan luka pasca pencabutan gigi. Metode: Jenis penelitian ini merupakan penelitian eksperimental laboratorium murni dengan rancangan post test only control group design. Subyek penelitian berupa 45 ekor tikus dibagi menjadi 3 kelompok, yaitu kelompok gel ekstrak daun binahong, kontrol negatif dan kontrol positif. Gigi insisivus sentral kiri rahang bawah dilakukan pencabutan gigi kemudian diaplikasikan gel ekstrak daun binahong 5%, CMC-Na 1% dan iod gliserin. Tikus diterminasi pada hari ke 3,5,7,14 dan 21 pasca dilakukan pencabutan gigi untuk selanjutnya dibuat preparat histologis dengan pengecatan hematoksilin eosin. Jumlah sel osteoblas dihitung dengan optilab dan software image raster. Hasil: Hasil uji parametrik one way anova menunjukan terdapat perbedaan jumlah osteoblas yang bermakna antar kelompok (p<0,05) pada masing-masing hari. Hasil LSD (Least Significance Different) menunjukkan terdapat perbedaan yang bermakna (p<0,05) pada hari ke 5,7, 14 dan 21 antara kelompok perlakuan dengan kontrol negatif dan terdapat perbedaan yang bermakna pada hari ke 7 dan 14 antara kelompok perlakuan dan kontrol positif. Simpulan: Gel ekstrak daun binahong konsentrasi 5% berpengaruh dalam meningkatkan jumlah sel osteoblas pasca dilakukan pencabutan gigi.Kata kunci: Pencabutan gigi, gel ekstrak daun binahong, osteoblas. ABSTRACTIntroduction: Tooth extraction is the most common treatment in dentistry. After tooth extraction, the bone in the socket will be damaged. The formation of new osteoblast characterises bone healing. Binahong leaves contain flavonoids, alkaloids, terpenoids, ascorbic acid, saponins, tannins and oleanolic acids. This research was aimed to investigate the effectivity of binahong (Anredera cordifolia) leaf extract gel with a concentration of 5% in increasing the number of osteoblasts at the tooth extraction wound healing process. Methods: This research was an experimental laboratory with a post-test only control group design. Forty-five rats were divided into 3 groups; binahong leaf gel group, negative control group, and positive control group. Mandibular left central incisive was extracted, then binahong leaf gel, 1% CMC-Na, and glycerin were applied on the wound socket in different groups. All rats then terminated at day 3, 5, 7, 14, and 21 post-extraction and processed for the histological examination then coloured by haematoxylin-eosin. The number of osteoblasts was calculated by Optilab and raster image software. Results: The ANOVA test result showed significant differences in the number of osteoblasts among groups (p<0.05) on each day. The LSD (Least Significance Different) test result showed significant differences (p<0.05) at day 5, 7, 14, and 21 post-extraction, between binahong leaf gel group compared to the negative control group, and also at day 7 and 14 post-extraction between binahong leaf gel group compared to the positive control group. Conclusion: Binahong leaf extract gel with a concentration of 5% is effective in increasing the number of osteoblast in the wound healing process after tooth extraction.Keywords: Tooth extraction, Binahong leaf extract gel, osteoblast.

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