scholarly journals Sintesis dan Karakterisasi Carbon Nanotube (CNt) dari Arang Kayu Jati serta Pemanfaatannya Sebagai Bahan Aktif Antibakteri

2020 ◽  
Vol 5 (2) ◽  
pp. 127
Author(s):  
Agung Rimayanto Gintu ◽  
Dhanang Puspita
Keyword(s):  
Antibacterial Activity ◽  
Activated Carbon ◽  
Carbon Nanotube ◽  
Strong Acid ◽  
Antibacterial Compounds ◽  
Antibacterial Compound ◽  
Activity Measurements ◽  
Absolute Viscosity

Carbone Nanotube (CNt) was one of antibacterial compound synthesized from carbon pr activated carbon. In this study threated the synthesis of CNt from teak charcoal used strong acid and sonication. The rendement of CNt synthesis showed potential result 37,6555±0,8038%. The characterization to the CNt showed density 1,0145±0,0037g/cc; Absolute Viscosity 1,06±0,0245P.a; Molecule weight 0,0007±0,00002g/mol. Threated the metal ionic doped to the CNt solution used Ag, Cu, Fe(II), Fe(III). Mg and Zn to increase the stabilitation and interaction. The characterization of CNt-Ag showed density 1,0072±0,0055g/cc; Absolute Viscosity 0,66±0,0400P.a; Molecule weight 0,0007±0,00004g/mol. The characterization of CNt-Cu showed density 1,0086±0,0040g/cc; Absolute Viscosity 0,56±0,0400P.a; Molecule weight 0,0007±0,00003g/mol. The characterization of CNt-Fe(II) showed density 1,0060±0,0013g/cc; Absolute Viscosity 0,76±0,0400P.a; Molecule weight 0,0007±0,000001g/mol. The characterization of CNt-Fe(III) showed density 1,0159±0,0042g/cc; Absolyte Viscosity 0,82±0,0200P.a; Molecule weight 0,0007±0,00003g/mol. Characterization of CNt-Mg showed density 0,9970±0,0008g/cc; Absolute Viscosity 0,64±0,0400P.a; Molecule weight 0,0007±0,000005g/mol. Characterization of CNt-Zn showed density 1,0013±0,0013g/cc; Absolute Viscosity 0,60±0,0447P.a; Molecule weight 0,0007±0,000005g/mol. Threated the mix to the CNt by another antibacterial compounds like Hydroxyapatite (HAp), Chitosan and Nano Chitosan. From the characterization of CNt-HAp obtained density 1,0008±0,0010g/cc; Absolute Viscosity 0,60±0,0316P.a; Molecule weight 0,0007±0,000007g/mol. Characterization CNt-Chitosan obtained density 0,9992±0,0019g/cc; Absolute Viscosity 0,42±0,0200P.a; Molecule weight 0,4343±0,0207g/mol. Characterization CNt-Nano Chitosan obtained density 1,0084±0,0002g/cc; Absolute Viscosity 0,50±0,0316P.a; Molecule weight 0,5170±0,3270g/mol. The results of antibacterial activity measurements against E.coli bacteries showed that all of the threatment to the chars produced the antibacterial activity in middle to strong range.

Antibacterial Activity of Traditional Medicine Scurrula atropurpurea (BL) DANS and their Endophytic Fungi

2020 ◽  
Vol 840 ◽  
pp. 205-213
Author(s):  
Elfita Elfita ◽  
Muharni Muharni ◽  
Mardiyanto Mardiyanto ◽  
Fitrya Fitrya ◽  
Feti Fera ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Bacterial Infections ◽  
Salmonella Typhi ◽  
Spectroscopy Analysis ◽  
Chromatography Method ◽  
Antibacterial Compounds ◽  
Activity Test ◽  
Antibacterial Compound ◽  
Promising Source

Scurrula atropurpurea known as benalu is a medicinal plant that has been used for the treatment of various diseases such as antibacterial. Plants with ethnomedicine history use to cure pathogenic bacterial infections and their endophytic fungi is a promising source of antibacterial compounds. This study aimed to compared the antibacterial activity of S. atropurpurea leaves and their endophytic fungi. The secondary metabolites were isolated from the leaves of S. atropurpurea and their endophytic fungi by the chromatography method. The antibacterial activity test was carried out by Kirby Bauer method against Salmonella typhi (IPCCCB.11.669) and Escherichia coli (ATCC 25922) as Gram (-) and Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) as Gram (+). The antibacterial compound from S. atropurpurea was determined by spectroscopy analysis as Quercetin-3-O-α-L-Rhamnopyranoside, while the antibacterial compound from endophytic fungi (strain BB1) as a lactone. Phylogenetic tree of strain BB1 has the highest homology with Neopestalotiopsis surinamensis strain CBS 450.74.

scholarly journals Antibacterial Activity ofPseudonocardiasp. JB05, a Rare Salty Soil Actinomycete againstStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Nesa Jafari ◽  
Reza Behroozi ◽  
Davoud Farajzadeh ◽  
Mohammad Farsi ◽  
Kambiz Akbari-Noghabi
Keyword(s):  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Scale Up ◽  
Diffusion Method ◽  
Hydroxyl Groups ◽  
Sephadex Column ◽  
Antibacterial Compounds ◽  
Antibacterial Compound ◽  
Layer Chromatography

Staphylococcus aureusis a Gram-positive bacterium that causes many harmful and life-threatening diseases. Some strains of this bacterium are resistant to available antibiotics. This study was designed to evaluate the ability of indigenous actinomycetes to produce antibacterial compounds againstS. aureusand characterize the structure of the resultant antibacterial compounds. Therefore, a slightly modified agar well diffusion method was used to determine the antibacterial activity of actinomycete isolates against the test microorganisms. The bacterial extracts with antibacterial activity were fractionated by silica gel and G-25 sephadex column chromatography. Also, the active fractions were analyzed by thin layer chromatography. Finally, the partial structure of the resultant antibacterial compound was characterized by Fourier transform infrared spectroscopy. One of the isolates, which had a broad spectrum and high antibacterial activity, was designated asPseudonocardiasp. JB05, based on the results of biochemical and 16S rDNA gene sequence analysis. Minimum inhibitory concentration for this bacterium was 40 AU mL−1againstS. aureus. The antibacterial activity of this bacterium was stable after autoclaving, 10% SDS, boiling, and proteinase K. Thin layer chromatography, using anthrone reagent, showed the presence of carbohydrates in the purified antibacterial compound. Finally, FT-IR spectrum of the active compound illustrated hydroxyl groups, hydrocarbon skeleton, and double bond of polygenic compounds in its structure. To the best of our knowledge, this is the first report describing the efficient antibacterial activity by a local strain ofPseudonocardia.The results presented in this work, although at the initial stage in bioactive product characterization, will possibly contribute toward thePseudonocardiascale-up for the production and identification of the antibacterial compounds.

scholarly journals Isolasi dan Karakterisasi Aktinomisetes Penghasil Antibakteri Enteropatogen Escherichia coli K1.1, Pseudomonas pseudomallei 02 05, dan Listeria monocytogenes 5407

2007 ◽  
Vol 3 (1) ◽  
pp. 15 ◽  
Author(s):  
Dwi Ningsih Susilowati ◽  
Ratih Dewi Hastuti ◽  
Erny Yuniarti
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Acetate Solution ◽  
Antibacterial Compounds ◽  
Isolation And Characterization ◽  
Antibacterial Compound ◽  
Group A ◽  
Pseudomonas Pseudomallei

<p>Isolation and Characterization of Actinomycetes Producing<br />Antibacterial Compound into Enteropatogenik<br />Escherichia coli K1.1, Pseudomonas pseudomallei 02 05<br />and Listeria monocytogenes 5407. Dwi N. Susilowati,<br />Ratih D. Hastuti, and Erny Yuniarti. The resistance of<br />bacterial pathogens to some antibacterial agents and side<br />effects of the antibacterial usage demanded discovery of<br />new effective, safe, and active antibacterial compounds.<br />Some pathogenic bacteria, such as enteropathogen Escherichia<br />coli (EPEC) that cause diarrhoea on children and<br />infants, Pseudomonas pseudomallei that cause melioidosis<br />on human and animal, and Listeria monocytogenes that<br />cause listeriosis on newly born babies mortality and death of<br />pregnant woman. Actinomycetes is the largest bacterial<br />group that produce antibiotics. More than 10,000 antibacterial<br />compounds had been discovered, two-third of<br />them were produced by this bacterial group. A study was<br />done to isolate and characterize Actinomycetes producing<br />antibacterial compounds effective against EPEC K1.1 and P.<br />pseudomallei 02 05. Soil samples were taken from 39<br />locations in Indonesia and 115 actinomycetes isolates were<br />obtained. Two of the isolates, i.e., isolate A3.5 that was<br />effective against P. pseudomallei 02 05 and isolate F6.1 that<br />was effective against EPEC K1.1 evaluated further. The<br />isolate A3.5 had an optimum time 72 hours to produce antibacterial<br />compound, while F6.1 took 96 hours. The antibacterial<br />compounds produced by both isolates were dissolve<br />in the a 70% ethyl acetate solution, but not in a 40oC<br />warm methanol solution because it is very dissolved. The<br />antibacterial compound extracted from the isolate A3.5 had<br />a similar effectiveness to antibiotics bacithracyn 10 unit and<br />neomycin 30 g. On the other hand, the antibacterial<br />compound extracted from isolate F6.1 had a similar effectiveness<br />to antibiotics colistin 10 g and doxyciclin 30 g.<br />Further identification of the isolates suggested that both of<br />them belongs to the genera Streptomyces.</p>

Characterization of Thermal Transport in Carbon Nanotube Yarns

2014 ◽  
Author(s):  
Jianli Wang ◽  
Sisi He ◽  
Jiajian Bao ◽  
Xing Zhang ◽  
Juekuan Yang ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Thermal Transport ◽  
Carbon Nanotube Yarns

Extraction and characterization of sea anemones compound and its Anti bacterial and hemolytic studies

2020 ◽  
Vol 10 (4) ◽  
pp. 93-97
Author(s):  
Anil Kumar A ◽  
Raja Sheker K ◽  
Naveen B ◽  
Abhilash G ◽  
Akila CR
Keyword(s):  
Antibacterial Activity ◽  
Focal Point ◽  
Haemolytic Activity ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Sea Anemones ◽  
Marine Plants ◽  
Heteractis Magnifica ◽  
Trididemnum Solidum

Seas assets that give us a variety of characteristic items to control bacterial, contagious and viral ailment and mostly utilized for malignancy chemotherapy practically from spineless creatures, for example, bryozoans, wipes, delicate corals, coelenterates, ocean fans, ocean bunnies, molluscs and echinoderms. In the previous 30 - 40 years, marine plants and creatures have been the focal point of overall endeavours to characterize the regular results of the marine condition. Numerous marine characteristic items have been effectively exceptional to the last phases of clinical preliminaries, including dolastatin-10, a group of peptides disengaged from Indian ocean rabbit, Dollabella auricularia. Ecteinascidin-743 from mangrove tunicate Ecteinascidia turbinata, Didemnins was isolated from Caribbean tunicate Trididemnum solidum and Conopeptides from cone snails (Conus sp.), and a developing number of up-and-comers have been chosen as promising leads for expanded pre-clinical appraisals. Sea anemones possess numerous tentacles containing stinging cells or cnidocytes. The stinging cells are equipped with small organelles known as nematocysts. The two species of sea anemones namely, Heteractis magnificaandStichodactyla haddoni, were collected from Mandapam coastal waters of Ramanathapuram district, Tamilnadu, India. The Nematocyst was collected and centrifuged, and the supernatant was lyophilized and stored for further analysis. The amount of protein from Heteractis Magnifica and Stichodactyla haddoni was estimated. The crude extract has shown haemolytic activity on chicken blood and goat blood. In the antibacterial activity of the sea anemone against six bacterial strains Staphylococcus aureus, Salmonella typhii, Salmonella paratyphii, Klebsiella pneumonia, Vibrio cholerae, Pseudomonas aeruginosa. Antibacterial activity of H. Magnifica and S.haddoni was measured as the radius of the zone of inhibition.

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