scholarly journals IDENTIFICATION OF BACTERIAL CONTAMINANTS ON GLASSES USED BY STUDENTS OF FACULTY OF MEDICINE, UNIVERSITAS AIRLANGGA, SURABAYA, INDONESIA CLASS OF 2016

2021 ◽  
Vol 31 (1) ◽  
pp. 18
Author(s):  
Hana Letitia Anjani ◽  
Marijam Purwanta ◽  
Maftuchah Rochmanti
Keyword(s):  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Eye Diseases ◽  
Pseudomonas Sp ◽  
Cotton Swab ◽  
Entire Surface ◽  
Bacterial Contaminants ◽  
Eye Infection ◽  
Continuous Use

Background: People nowadays suffer from refractive disorder that declines the vision. The most common therapy to overcome this problem is to use glasses. The continuous use of glasses and the difficulties on disinfecting the entire surface can cause bacterial contamination. Those bacteria can cause various eye diseases. This fact pushed the researcher to find out if there were bacterial contaminants on glasses used by students of Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia, class of 2016. Objective: To prove the species of bacterial contaminants and pathogenic bacteria on glasses used by students of Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia class of 2016. Materials and Methods: Cotton swab moistened in sterile distilled water was swabbed to 30 glasses on its inside lens and the part touching the nose. The sample was then inoculated on blood agar and Mac Conkey, and incubated for 24 hours in 37oC. Then, the isolates were identified macroscopically and microscopically. Results: The whole sample that had been examined was contaminated with pathogenic bacteria with risk of causing eye diseases. Those bacteria were identified as Bacillus sp. (50%), Pseudomonas sp. (46%), Staphylococcus aureus (20%), and Staphylococcus epidermidis (16%). Conclusion: The result of this research showed that there was contamination by bacteria on glasses used by students of Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia class of 2016. All bacteria found were pathogenic to the eye. This showed that glasses could become a reservoir of the pathogenic bacteria that could potentially cause recurring eye infection.

scholarly journals IDENTIFICATION OF BACTERIAL CONTAMINANTS ON GLASSES USED BY STUDENTS OF FACULTY OF MEDICINE UNIVERSITAS AIRLANGGA CLASS OF 2016

2021 ◽  
Vol 31 (1) ◽  
pp. 21
Author(s):  
Hana Letitia Anjani ◽  
Marijam Purwanta ◽  
Maftuchah Rochmanti
Keyword(s):  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Eye Diseases ◽  
Pseudomonas Sp ◽  
Cotton Swab ◽  
Entire Surface ◽  
Bacterial Contaminants ◽  
Eye Infection ◽  
Continuous Use

Background: People nowadays suffer from refractive disorder that declines the vision. The most common therapy to overcome this problem is to use glasses. The continuous use of glasses and the difficulties on disinfecting the entire surface can cause bacterial contamination. Those bacteria can cause various eye diseases. This fact pushed the researcher to find out if there were bacterial contaminants on glasses used by students of Faculty of Medicine Universitas Airlangga class of 2016. Objective: To prove the species of bacterial contaminants and pathogenic bacteria on glasses used by students of Faculty of Medicine Universitas Airlangga class of 2016. Material and Method: Cotton swab moistened in sterile distilled water was swabbed to 30 glasses on its inside lens and the part touching the nose. The sample was then inoculated on blood Agar and Mac Conkey, and incubated for 24 hours in 37°C. Then, the isolates were identified macroscopically and microscopically. Results: The whole sample that had been examined was contaminated with pathogenic bacteria with risk of causing eye diseases. Those bacteria were identified as Bacillus sp. (50%), Pseudomonas sp. (46%), Staphylococcus aureus (20%), and Staphylococcus epidermidis (16%). Conclusion: The result of this research showed that there was contamination by bacteria on glasses used by students of Faculty of Medicine Universitas Airlangga class of 2016. All bacteria found were pathogenic to the eye. This showed that glasses could become a reservoir of the pathogenic bacteria that could potentially cause recurring eye infection.

Isolation of Pathogenic Bacteria from some Male Barbershops in the City of Nasiriyah

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Fadil G. Alswedi ◽  
Amany S H Jaber
Keyword(s):  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Bacterial Species ◽  
Bacterial Isolates ◽  
Bacterial Isolation ◽  
Potential Pathogen ◽  
Colonial Morphology ◽  
Spread Of Infection ◽  
The City

A study was conducted to evaluate bacterial contamination in hairdressing and beauty salons in Thi-Qar at Alnasiriyah city. Samples were collected from ten different salons. The samples were collected from scissors,comb, razor, dryer, sink, and table tools. The isolates obtained were examined and identified using microscopic examination, colonial morphology and biochemical characteristics. Six bacterial species were isolated and identified. The bacterial isolates include Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus spp, Micrococcus Spp,Enterococusspp and Enterobacter. Isolated from some men's salons and shaving tools and the highest frequency of bacteria was in the salon of Nawras by 15%, Salon Ahmed by 12.5% , more bacterial isolation of shaving tools,sink,razor and the presence of this potential pathogen is an indication that hairdressing salons could be contributing to the spread of infection.

scholarly journals Optimized genotyping method for identification of bacterial contaminants in pharmaceutical industry

2016 ◽  
Vol 66 (2) ◽  
pp. 289-295
Author(s):  
Borche Stamatoski ◽  
Miroslava Ilievska ◽  
Hristina Babunovska ◽  
Nikola Sekulovski ◽  
Sasho Panov
Keyword(s):  
Pharmaceutical Industry ◽  
Dna Sequencing ◽  
Bacterial Contamination ◽  
Raw Materials ◽  
Pcr Primers ◽  
Rrna Gene ◽  
Bacterial Identification ◽  
Bacterial Contaminants ◽  
Microbiological Control ◽  
Selection Of

AbstractMicrobiological control is of crucial importance in the pharmaceutical industry regarding the possible bacterial contamination of the environment, water, raw materials and finished products. Molecular identification of bacterial contaminants based on DNA sequencing of the hypervariable 16SrRNA gene has been introduced recently. The aim of this study is to investigate the suitability of gene sequencing using our selection of PCR primers and conditions for rapid and accurate bacterial identification in pharmaceutical industry quality control.DNA was extracted from overnight incubated colonies from 10 bacterial ATCC strains, which are common contaminants in the pharmaceutical industry. A region of bacterial 16SrRNA gene was analyzed by bidirectional DNA sequencing. Bacterial identification based on partial sequencing of the 16SrRNA gene is the appropriate method that could be used in the pharmaceutical industry after adequate validations. We have successfully identified all tested bacteria with more than 99 % similarity to the already published sequences.

scholarly journals DETECTION OF POTENTIALLY PATHOGENIC BACTERIA IN THE BRACKISH RIVERS FLOWING INTO THE ELTON LAKE BY HIGH-THROUGHPUT SEQUENCING

2018 ◽  
pp. 87-95 ◽  
Author(s):  
E. A. Selivanova ◽  
Yu. A. Khlopko ◽  
N. E. Gogoleva ◽  
A. O. Plotnikov
Keyword(s):  
High Throughput ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
High Throughput Sequencing ◽  
Pore Diameter ◽  
Pathogenic Microorganisms ◽  
Membrane Filters ◽  
Marine Habitats ◽  
Dna Libraries ◽  
Enzymatic Lysis

Aim. To indicate potentially pathogenic bacteria in plankton of the brackish rivers flowing into the Elton Lake by high-throughput sequencing of 16S ssuRNA gene. Materials and methods. The water samples from brackish rivers Lantsug and Chernavka, flowing into the Elton Lake, were taken up in a volume of 50 ml, filtered through membrane filters (pore diameter - 0.22 pm). Total DNAwas obtained by phenol-chloroform extraction with preliminary homogenization and enzymatic lysis. DNA libraries for sequencing were created by protocol Illumina with primers to a variable V3-V4 region of 16S ssuRNA gene. Sequencing was performed on a platform MiSeq («Illumina», США). Results.There were found the phylotypes of potentially pathogenic bacteria of Proteobacteria phylum from the families Enterobacteriaceae, Pseudomonadaceae, Campylobacteraceae, Vibrionaceae, Aeromonadaceae, Moraxellaceae, Legionellaceae, Alcaligenaceae, Campylobacteraceae, and also of Firmicutes, Bacteroidetes, Actinobacteria phyla in the plankton samples of the brackish rivers. Probable source of bacterial contamination is large and small cattle. Conclusion. These data demonstrate that the continental brackish waters, along with freshwater and marine habitats perform a reservoir function to potentially pathogenic microorganisms. High-throughput sequencing can be used to screen the presence of pathogens in water.

scholarly journals Identification of Pathogenic Bacteria in Cyprinus carpio Reared in Floating Cages

2021 ◽  
Vol 26 (1) ◽  
pp. 26
Author(s):  
Erikson Sahala Pardamean ◽  
Henni Syawal ◽  
Morina Riauwaty
Keyword(s):  
Water Quality ◽  
Cyprinus Carpio ◽  
Pathogenic Bacteria ◽  
Survey Method ◽  
Fish Parasite ◽  
Pseudomonas Sp ◽  
Fish Sample ◽  
Purposive Sampling ◽  
Fish Samples ◽  
Cultured Fish

Pathogenic bacteria is disease causing microorganisms that can attack fish and can cause mass death in cultured fish. The purpose of this study was to identify the types of pathogenic bacteria Cyprinus carpio that were reared in floating cages. This study used a survey method that is purposive sampling and the fish sample were obtained from, PLTA Koto Panjang. the fishes were identified in the Fish Parasite and Disease Laboratory, Fish and Marine Faculty, University of Riau. Fish samples used were 15-20 cm goldfish totaling 12 tails with 3 times taken from 4 different cages. The kidneys organ were examined and to find out the type the bacteria. Result shown identified of pathogenic bacteria consist of 3 types of bacteria namely Aeromonas sp., Pseudomonas sp., and Edwardsiella sp. Water quality during the study was Temperature: 27-300C, pH 6-7, DO 3.67-4.28 ppm, and ammonia 0.048-0.64 mg/L.

scholarly journals Bacterial Contamination of Street Vended Food Pani Puri Available in Janakpurdham, Dhanusha

2019 ◽  
Vol 6 ◽  
pp. 70-75
Author(s):  
Nagendra Prasad Yadav ◽  
Rakesh Kumar Yadav ◽  
Satyam Prakash
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Food Samples ◽  
Microbial Count ◽  
Microbiology Laboratory ◽  
Dilution Technique ◽  
Bacillus Spp ◽  
Klebsiella Spp

Objectives: To determine the level of bacterial contamination in food (Panipuri) available in market of Janakpur, Dhanusha, Nepal. Methods: Total 120 samples of Panipuri (60 solid matters and 60 masalaa Pani) collected aseptically from the market were transported to the microbiology laboratory and processed for microbial count by serial dilution technique. Microbial load and presence of pathogen were detected in each sample. Results: Analysis of the food samples revealed that 70% of Panipuri samples had high loads of bacterial pathogens such as Escherichia coli, Staphylococcus aureus, Klebsiella spp, Pseudomonas spp, Bacillus spp.  Conclusion: This study depicted that the street vended food Panipuri of Janakpur are highly contaminated with pathogenic bacteria which can cause health risk to consumers. Thus, it is suggested that regular monitoring of the quality of street foods must be practiced to avoid any food bone illness in future.

scholarly journals CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

Science ◽  
2008 ◽  
Vol 322 (5909) ◽  
pp. 1843-1845 ◽  
Author(s):  
Luciano A. Marraffini ◽  
Erik J. Sontheimer
Keyword(s):  
Antibiotic Resistance ◽  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Target Sequence ◽  
Crispr Interference ◽  
Multiple Routes ◽  
Short Palindromic Repeat ◽  
Self Splicing

Horizontal gene transfer (HGT) in bacteria and archaea occurs through phage transduction, transformation, or conjugation, and the latter is particularly important for the spread of antibiotic resistance. Clustered, regularly interspaced, short palindromic repeat (CRISPR) loci confer sequence-directed immunity against phages. A clinical isolate ofStaphylococcus epidermidisharbors a CRISPR spacer that matches thenickasegene present in nearly all staphylococcal conjugative plasmids. Here we show that CRISPR interference prevents conjugation and plasmid transformation inS. epidermidis. Insertion of a self-splicing intron intonickaseblocks interference despite the reconstitution of the target sequence in the spliced mRNA, which indicates that the interference machinery targets DNA directly. We conclude that CRISPR loci counteract multiple routes of HGT and can limit the spread of antibiotic resistance in pathogenic bacteria.

scholarly journals Keratinocyte Expression of Human β Defensin 2 following Bacterial Infection: Role in Cutaneous Host Defense

2003 ◽  
Vol 10 (1) ◽  
pp. 161-166 ◽  
Author(s):  
James G. H. Dinulos ◽  
Laurel Mentele ◽  
L. Page Fredericks ◽  
Beverly A. Dale ◽  
Gary L. Darmstadt
Keyword(s):  
Host Defense ◽  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Skin Surface ◽  
Immune Defense ◽  
Skin Infections ◽  
Reverse Transcription Pcr ◽  
Relative Tolerance ◽  
Disease Induction ◽  
Stimulation Of

ABSTRACT Human β defensin 2 (hβD-2) is thought to play an important role in cutaneous immune defense. We hypothesized that (i) keratinocyte expression of hβD-2, measured by reverse transcription-PCR, would be upregulated in response to challenge with pathogenic bacteria, particularly highly adherent strains of Streptococcus pyogenes and Staphylococcus aureus, and (ii) hβD-2 would have potent antimicrobial activity against pathogenic but not commensal organisms. Expression of hβD-2 was induced consistently by S. aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa, whereas strains of S. pyogenes were poor and variable inducers of hβD-2. No correlation was found between levels of bacterial adherence and keratinocyte expression of hβD-2. S. pyogenes was significantly more sensitive to killing by hβD-2 than S. epidermidis. We conclude that the ability to induce hβD-2 expression in combination with sensitivity to its antimicrobial effects may contribute to the rarity of skin infections with the gram-negative bacterial organisms, whereas lack of stimulation of hβD-2 expression by S. pyogenes may be important in its ability to evade innate defenses and cause skin disease. Induction of expression of hβD-2 but relative tolerance to it may enable S. epidermidis to survive on the skin surface and modulate hβD-2 expression when the stratum corneum barrier is disrupted.

scholarly journals Bacteriological quality of dry powder milk available in local markets of Bangladesh

2018 ◽  
Vol 4 (3) ◽  
pp. 267-273
Author(s):  
Nazia Afrin ◽  
Rasheda Yasmin Shilpi
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Count ◽  
Pseudomonas Sp ◽  
Dry Powder ◽  
Milk Samples ◽  
Bacteriological Quality ◽  
Powder Milk ◽  
Surrounding Areas ◽  
Almost All

Milk and milk powders are very rich in several nutrients and relished by everybody throughout the world. The present study was undertaken with the aim of investigating the bacteriological quality of locally available dry powder milk in Bangladesh. A total number of eight powder milk samples were collected from Gazipur city and its surrounding areas during the period from January 2017 to February 2017. The analysis comprised of enumeration of total viable bacterial count (TVBC), isolation of bacterial isolates and identification of pathogenic bacteria. Almost all the powder milk samples showed the total aerobic heterotrophic bacterial (TAHB) level above the standard acceptable range (>104 CFU/g). Both gram positive and gram negative pathogenic bacteria viz. Staphylococcus aureus, Staphylococcus epidermidis, Bacillus, pseudomonas sp. and Enterobacter sp. were isolated from studied samples through morphological and biochemical characteristics. Pseudomonas sp. were confirmed by API 20E. These results highlighted the necessity to maintain appropriate sanitary and hygienic measures at each critical point in order to safeguard consumers from food borne pathogens.Asian J. Med. Biol. Res. September 2018, 4(3): 267-273

scholarly journals Ants (Hymenoptera: Formicidae) as Potential Mechanical Vectors of Pathogenic Bacteria in a Public Hospital in the Eastern Amazon, Brazil

2020 ◽  
Vol 57 (5) ◽  
pp. 1619-1626
Author(s):  
Leonardo Espíndola do Nascimento ◽  
Raquel Rodrigues Amaral ◽  
Ricardo Marcelo dos Anjos Ferreira ◽  
Diogo Vitor Soares Trindade ◽  
Rafael Espíndola do Nascimento ◽  
...  
Keyword(s):  
Public Hospital ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Bacterial Species ◽  
Brain Heart Infusion ◽  
Hospital Environment ◽  
Hospital Environments ◽  
Microbiological Analyses ◽  
Amazonian Region ◽  
Dominant Ant

Abstract Studies related to ants found in hospital environments have aroused interest in their role as mechanical vectors of pathogenic microorganisms. The objective of the current research was to determine the species composition and bacterial contamination of ant species found in a public hospital in the eastern Amazonian region. Ants were captured using bait containing honey and sterilized sardines in 15 locations within the Macapá Emergency Hospital, Amapá. Ants were identified morphologically using specific keys. Bacteria were first inoculed in a Brain Heart Infusion broth and then plated on 5% Agar with blood or MacConkey media. Bacterial species were identified through biochemical procedures. In total, 9,687 ants were collected, with 69.8% from the dry season and 30.2% from the rainy season. Nine species of ants were identified belonging to three subfamilies: the Monomorium pharaonis (Linnaeus 1758) being the most common, comprising 39.2% of the total specimens. Only one ant species was found in each bait, facilitating microbiological analyses. In total, 92 bacteria isolates were identified comprising 12 species. Pseudomonas aeruginosa Schroeter 1872 (Pseudomonadales: Pseudomonadaceae) was pathogenic bacteria, most frequently isolated, comprising 10.9% of the positive samples. The most contaminated ant in the study was M. pharaonis with 38.3%. It was the dominant ant species in this hospital environment. Its wide prevalence, forage day and night of this vector in hospital facilitated bacterial contamination. The presence of bacteria on ants may be associated with the dissemination of pathogens which cause hospital infections, making pest control a necessity in these institutions.

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