scholarly journals THE EFFECT OF BINJAI (Mangifera caesia) LEAVES EXTRACT GEL TO FIBROBLAST CELL NUMBER ON INCISION WOUND

2021 ◽  
Vol 6 (2) ◽  
pp. 166
Author(s):  
Noor Hildayanti ◽  
Irham Taufiqurrahman ◽  
Renie Kumala Dewi
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Fibroblast Cell ◽  
Negative Control Group ◽  
Cell Number ◽  
Negative Control ◽  
Control Group ◽  
Meaningful Difference ◽  
Male Wistar Rats ◽  
Incision Wound

Background: The ethanol extract of binjai leaves contains bioactive compounds that are saponin, tannin, triterpenoid, steroid, alkaloid and flavonoid which potentially as an anti-inflammation, anti-bacterial, and antioxidant. It can fasten and control the process of scar healing. The binjai leaves extract was made into gel preparation. The binjai leaves extract gel can affect fibroblast cell number during the proliferation phase. Purpose: To analyze the effect of binjai (Mangifera caesia) leaves extract gel to the fibroblast cell number on male wistar rats (Rattus norvegicus) incision wound.  Methods: This research design was a true laboratory experimental research with post-test only with control group design which used 24 male wistar rats and divided into 4 groups: the negative control group that was given the placebo gel and the treatment group that was given the binjai leaves extract gel with 5%, 10%, and 15% concentrations. Results: The result of Two-way ANOVA statistic test shows that there is a meaningful difference in the treatment group on the 7th day (p=0,000) and on the 14th day (p=0,000). The Post-Hoc Bonferroni test for fibroblast cell number on the 7th and 14th day shows that there is a meaningful difference (p<0,05) between the negative control group and the treatment group. Conclusion: Binjai leaves extract gel have an effect to the fibroblast cell number on male wistar rats incision wound with the most effective concentration of 15%. Keywords: Binjai leaves extract gel, Fibroblast cell number,  Flavonoid, Incision wound healing

Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Solomon E. Owumi ◽  
Jeremiah O. Olugbami ◽  
Andrew O. Akinnifesi ◽  
Oyeronke A. Odunola
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Telfairia Occidentalis ◽  
Male Wistar Rats

Abstract Objectives Diethylnitrosamine (DEN) is found in workplaces, processed meats, tobacco smoke, whiskey, etc. It is capable of forming DNA-adducts. Fluted pumpkin (Telfairia occidentalis [To]) is a medicinal plant, and its herbal preparations have been employed variously in ethnomedicine. Furthermore, it has been reported to possess anti-oxidant, anti-cancer, anti-inflammatory properties. We investigated the possible mitigating effect of the leaf paste of To on DEN-induced deleterious effects in male Wistar rats. Methods Forty-five rats weighing between 100 and 150 g were equally divided into nine groups and treated thus: Group 1 (negative control), Group 2 (0.05 mg/kg carboxymethyl cellulose [CMC] daily), Group 3 (positive control, 25 mg/kg bw DEN administered intraperitoneally thrice per week), Group 4 (25 mg/kg bw quercetin [QUE] daily alone), Groups 5 and 6 (100 and 200 mg/kg bw To daily, respectively), Group 7 (25 mg/kg bw DEN and QUE), Groups 8 and 9 (25 mg/kg bw DEN with 100 and 200 mg/kg bw To, respectively). Blood glucose levels, liver damage biomarkers (aspartate aminotransferase [AST], alanine aminotransferase [ALT] and gamma-glutamyltransferase [γ-GT]), frequency of micronucleated polychromatic erythrocyte (mPCEs), and liver histology were assessed. Results DEN significantly (p<0.05) increased blood glucose levels, activities of ALT, AST and γ-GT, and frequency of mPCEs. Histologically, DEN caused a severe architectural anarchy. However, the intervention groups demonstrated the remarkable protective properties of To by ameliorating the adverse effects caused by DEN. Conclusions Taken together, the leaf paste of To is capable of mitigating DEN-induced hepatotoxicity and clastogenicity in male Wistar rats.

scholarly journals Gambaran histologik hati tikus Wistar yang diberi jus tomat setelah diinduksi monosodium gluatamat

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Christantio Legoh ◽  
Martha M. Kaseke ◽  
Taufiq F. Pasiak
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Fatty Degeneration ◽  
Negative Control Group ◽  
Negative Control ◽  
Tomato Juice ◽  
Control Group ◽  
Group I ◽  
Group Ii

Abstract: Monosodium glutamate (MSG) is one of the ingredients used as flavour additive. However, excessive consumption of MSG could damage the liver. This study was aimed to obtain the impact of MSG exposure on histopathological findings of the liver of Wistar rats administered with tomato juice. This was an experimental study with the post-test only control group design. Subjects were Wistar rats divided into three groups, as follows: the negative control group, treatment group I, and treatment group II. The negative control group was given AD2 pellets; the treatment group I was given AD2 pellets and MSG, while the treated group II was given MSG and tomato juice. This study was conducted for 14 days. The histopathological examination of the treatment group I showed fatty degeneration of hepatocytes and infiltration of inflammatory cells meanwhile of the treatment group II, there was less fatty degeneration of hepatocytes compared to the treatment group I and no inflammatory cells. Moreover, there was no fatty degeneration and inflammatory cells in the negative control group. Conclusion: Wistar rats treated with MSG showed fatty degeneration of hepatocytes and infiltration of inflammatory cells which could be reduced by administration of tomato juice along with MSG.Keywords: tomato juice, MSG Abstrak: Monosodium glutamat (MSG) merupakan salah satu bahan makanan yang sering digunakan sebagai penyedap rasa namun konsumsi MSG berlebihan dapat merusak hati. Penelitian ini bertujuan untuk mengetahui pengaruh pajanan MSG terhadap gambaran histologik hati tikus Wistar dengan dan tanpa disertai pemberian jus tomat. Jenis penelitian ialah eksperimental dengan post-test only control group design. Tikus dibagi menjadi 3 kelompok, yaitu kelompok kontrol negatif, kelompok perlakuan I, dan kelompok perlakuan II. Kelompok kontrol negatif hanya diberikan pelet AD2; kelompok perlakuan 1 diberikan pelet AD2 dan MSG; kelompok perlakuan 2 diberikan MSG dan jus tomat. Penelitian ini dilakukan selama 14 hari. Hasil pengamatan gambaran histologik hati tikus Wistar pada kelompok perlakuan I mendapatkan adanya degenerasi lemak pada hepatosit dan sel-sel radang PMN. Pada kelompok perlakuan 2 masih ditemukan adanya degenerasi lemak pad hepatosit tetapi dengan jumlah yang lebih kurang daripada kelompok perlakuan I, serta tidak ditemukan sel-sel radang PMN. Pada kelompok kontrol negatif tidak ditemukan adanya degenerasi lemak pada hepatosit dan sel radang. Simpulan: Pada tikus wistar dengan pemberian MSG terdapat degenerasi lemak pada hepatosit dan adanya sel-sel radang. Pemberian jus tomat bersamaan dengan pemberian MSG berefek menurunkan terjadinya degenerasi lemak pada hepatosit dan tidak disertai sel-sel radang.Kata kunci: jus tomat, MSG

scholarly journals Nephroprotective effect of soy (Glycine Max (L.) Merr.) flour against diazinon-induced renal toxicity in rats

2020 ◽  
Vol 39 (3) ◽  
pp. 192
Author(s):  
Desie Dwi Wisudanti ◽  
Rena Normasari ◽  
Firman Herdiyana
Keyword(s):  
Glycine Max ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Negative Control ◽  
Soy Flour ◽  
Control Group ◽  
Treatment Groups ◽  
Male Wistar Rats ◽  
Glycine Max L ◽  
Nephroprotective Effect

<strong>Background</strong><br />Diazinon is a widely used pesticide to eradicate agricultural pests globally with the potential to cause harmful effects on humans by generating free radicals and depleting endogenous antioxidants. Soy flour possesses high antioxidant effect. This study evaluated the nephroprotective effect of soy flour  on diazinon-induced nephrotoxicity in rats. <br /><br /><strong>Methods</strong><br />An experimental laboratory study was carried out involving 30 male Wistar rats were randomly divided into five groups of 6 rats. The normal control (Kn) and the negative control (K-) groups <em>received</em> normal saline, the treatment groups (K1, K2, K3) received 10%, 15%, and 20% soy flour in distilled water, respectively, on day 1 to day 28. Subsequently, for five days, all groups received diazinon 40 mg/kg, except for the normal control group. Blood urea nitrogen (BUN) and creatinine levels were measured at the end of the study. The Kruskal Wallis test with the post hoc Mann Whitney U test were used to analyze the data. <br /><br /><strong>Results</strong><br />Blood urea nitrogen and creatinine levels were lower in all treatment groups (K1, K2, K3) compared to the negative control group (K-), while the K3 level was the lowest (24.28 ± 1.56 mg/dL and 0.93 ± 0.14 mg/dL).  There was a significant decrease in BUN and creatinine levels in the experimental rats of the 15% and 20% soy flour groups (K2, K3) compared to the negative control group (K-).<br /><br /><strong>Conclusion</strong><br />Administration of soy flour (Glycine max (L.) Merr.) had a nephroprotective effect in preventing increases in BUN and creatinine levels of diazinon-induced nephrotoxic male Wistar rats.

scholarly journals Evaluation of the effect of different concentrations of Arsenicum album 6cH on intoxicated rats

2021 ◽  
Vol 8 (28) ◽  
pp. 119-127
Author(s):  
Olney Leite Fontes ◽  
Fátima Cristiane Lopes Goularte Farhat ◽  
Amarilys Toledo Cesar ◽  
Marilisa Guimarães Lara ◽  
Maria Imaculada Lima Montebelo ◽  
...  
Keyword(s):  
Atomic Absorption Spectroscopy ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Groups ◽  
Before And After ◽  
Male Wistar Rats

Aims: Homeopaths diverge on the concept of dose, i.e. the amount of drug that a patient must take to alter his or her state of disease. In order to stimulate reflections on this concept, this study sought to evaluate in vivo the effect of different concentrations of Arsenicum album 6cH prepared according to homeopathic pharmacotechnics. Methods: male Wistar rats were intoxicated with arsenic and then treated with Arsenicum album 6cH and Arsenicum album 6cH diluted at 1%, administered orally. The amount of arsenic retained in the animals’ organism and that eliminated by urine were measured through atomic absorption spectroscopy. Samples of urine were collected before and after intoxication and during treatment. The positive control group (intoxicated animals) and the negative control group (non-intoxicated animals) received only the vehicle used in the preparation of the medicine. Results: Groups treated with Arsenicum album 6cH and Arsenicum album 6cH diluted at 1% eliminated significant amounts of arsenic when compared to the control groups. The group treated with Arsenicum album 6cH eliminated significantly higher amounts of arsenic than the group treated with the diluted medicine at 1%. Conclusion: results suggest that Arsenicum album 6cH should not be diluted as not to compromise its effectiveness in the treatment of rats intoxicated with arsenic.

scholarly journals The mitigating effect of Ananas comosus on aluminum-induced oxidative stress on the testes of adult male Wistar rats

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Bankole J. Leko ◽  
Solomon T. Olawuyi ◽  
Lawrence U. Okon
Keyword(s):  
Oxidative Stress ◽  
Aluminum Chloride ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Negative Control ◽  
Ananas Comosus ◽  
Control Group ◽  
Distilled Water ◽  
Pineapple Juice ◽  
Male Wistar Rats

Abstract Background The mitigating effect of Ananas comosus (pineapple juice) extract on an aluminum-induced testicular toxicity in male Wistar rats was examined in this study. Thirty healthy adult male Wistar rats with an average weight of 200 g were grouped into six groups; distilled water and 1 ml of pineapple juice extracts were administered to the control and treated animals respectively for 3 weeks. The control group was given rat pellets and distilled water. Negative control was given 100mg/kg/d Aluminum Chloride and pellets; Group 1 was given 100mg/kg/d and 1ml of pineapple juice in distilled water orally; Group 2 was given 100mg/kg/d Aluminum Chloride and 1.5 ml of in distilled water orally; group 3 was given 100 mg/kg/day aluminum chloride and 2 ml of pineapple juice in distilled water orally; group 4 was given 100 mg/kg/day aluminum chloride and 2.5 ml of pineapple juice in distilled water orally. Testicular histology, semen parameters, and testosterone were assessed. Results This study showed a significant (P < 0.05) decrease in testicular volume, motile sperm count, concentration, total count, progressive evaluation, and morphology in the negative control group relative to the normal control and extract control groups. In the groups co-treated with aluminum chloride and Ananas comosus extract, there was improvement in sperm volume, motility, total count, progressive assessment, and morphology. There was also a statistical decrease (P < 0.05) in testosterone hormone in the negative control group, but there was an increase in the aluminum chloride and Ananas comosus extract co-treated groups. Similarly, in co-treated aluminum chloride and Ananas comosus extract, the degenerative seminiferous tubule histoarchitecture due to aluminum chloride in the negative control group was enhanced. Conclusion Based on this current study, it was evident that aluminum chloride induced oxidative stress and retarded reproduction in males whereas Ananas comosus mitigated reproduction in males by improving sperm parameters and microarchitecture of the testes.

scholarly journals EFEK NIGELLA SATIVA OIL TERHADAP UKURAN DIAMETER ULKUS TRAUMATIKUS PADA MALE WISTAR RATS SECARA IN VIVO

2016 ◽  
Vol 3 (2) ◽  
pp. 94
Author(s):  
Linda Septiana ◽  
Ratnawati Hendari ◽  
Erwid Fatchur Rahman ◽  
Diyah Fatmasari
Keyword(s):  
Wistar Rats ◽  
Nigella Sativa ◽  
Pathological Condition ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Epithelial Tissue ◽  
Nigella Sativa Oil ◽  
Male Wistar Rats

Background: Ulcer is a pathological condition characterized by loss of epithelial tissue. Ulcer will experience healing within 2 weeks after trauma source is removed. Nigella sativa Oil has been known to heal wounds. The purpose of this study was to evaluate the effect of NSO on healing of ulcers in male wistar rats from the use of chemicals.Method: This study design was quasi-experimental methods. Ten male wistar rats were exposing the H2O2 on the mandibular anterior gingiva. divided into negatif control group and NSO group. NSO group treated twice daily for 10 days. Observaton wound size was measured on 0, 3,7, 10 days. The results were analayzed with Repeated Anova test and LSD test.Result: The observations difference diameter of traumatic ulcer negative control group and NSO on 0-10 days was 1.458 mm and 2.182 mm. The results of data analysis showed that there are significant differences (p<0,05) between negatif control group and NSO.Conclusion: NSO has an influence of the size reduction of the diameter of traumatic ulcers.

scholarly journals Comparison of The Number of Macrophage in Full Thickness Wound Incision between Merremia mammosa Extract Treatment and NaCl in Male Wistar Rats

1970 ◽  
Vol 1 (1) ◽  
pp. 9
Author(s):  
Fajar Kurniawan Hidayat ◽  
Ulfa Elfiah ◽  
Kristianningrum Dian Sofiana
Keyword(s):  
Diabetes Mellitus ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Field Of View ◽  
Control Group ◽  
Full Thickness ◽  
Significant Difference ◽  
Male Wistar Rats

The incidence of diabetes mellitus in Indonesia continues to increase. There are some complications in the diabetic condition, one of which is diabetic ulcer. Diabetic ulcers in diabetes patient can increase the risk of amputation and expensive treatment costs, so the alternative treatmeant such as Merremia mammosa wich has antiinflamatory and antidiabetic is needed. This study aimed to determine the comparison of the number of macrophage in the incisional wound in hyperglycemic male wistar rats between treatment with Merremia mammosa extract and NaCl. The in vivo test was done by creating wound incision on the mice backs and treated with gentamycin ointment 5% in a positive control group, NaCl in a negative control group and Merremia mammosa extract in a dose of 100m), 200mg, 400mg in treatment group. The result obtained by counting the number of macrophage in histopatholgy examination. The result showed the number of macrophage were 0,36 cells/field of view in a negative control group, 0,52 cells/field of view in a positive control group, 0,48 cells/field of view in all Merremia mammosa treatment groups. The data analysis showed no significant difference with p-value of 0,729. In conclusion, there was no significant difference between the used of Merremia mammosa extract and NaCl on full thickness incisional wounds of hyperglycemic male wistar rats. Keywords: Diabetes Mellitus, Merremia mammosa extract, incisional wound

scholarly journals ANTIHYPERURICEMIC ACTIVITY OF ETHANOL EXTRACT OF SYZYGIUM CUMINI LEAVES ON POTASSIUM OXONATED-INDUCED RATS

2018 ◽  
Vol 11 (13) ◽  
pp. 132
Author(s):  
Mona Asiah ◽  
Rosidah Rosidah ◽  
Rosidah Rosidah ◽  
Yuandani Yuandani ◽  
Yuandani Yuandani
Keyword(s):  
Uric Acid ◽  
Serum Uric Acid ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Syzygium Cumini ◽  
Male Wistar Rats ◽  
Potassium Oxonate

Objective: This study was conducted to investigate the effect of extract of Syzygium cumini on serum uric acid levels of rats induced by potassium oxonate.Methods: A total of 30 male Wistar rats were randomly divided into six groups, consist of one normal group (without treatment) and five groups that were induced by potassium oxonate 250 mg/kg bw intraperitoneally for 7 days. The ethanol extract of S. cumini leaves dose (100, 200, and 400 mg/kg bw) was administered orally 1 h after the potassium oxonate exposure during 7 days. On day 8, blood samples were collected for serum uric acid determination.Result: Ethanol extract of S. cumini leaves at doses of 100 mg/kg bw, 200 mg/kg bw, and 400 mg/kg bw significantly reduced serum uric acid as compared to negative control group of carboxymethylcellulose 0.5%.Conclusion: Ethanol extract of S. cumini leaves can be used as antihyperuricemic agent.

scholarly journals UJI EFEK ANTIPIRETIK EKSTRAK DAUN BELUNTAS (Pluchea indica (L.) Less.) PADA TIKUS WISTAR (Rattus norvegicus) JANTAN

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Kallan A. B. Pulio ◽  
Christi Mambo
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Intervention Group ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Slight Reduction ◽  
Antipyretic Effect ◽  
Male Wistar Rats ◽  
Hb Vaccine

Abstract: Background: Pluchea indica Less. is one of the widely known and widely distributed native potential medicinal plants in Indonesia. Objectives: To determine the antipyretic effect of pluchea indica Less. abstract on male Wistar rats (Rattus norvegicus). Metods: This is an experimental study conducted on 15 male Wistar rats with weight ranging from 150-200 gram. Study animals were divided into 5 groups, namely positive control group, negative control group and three intervention group to whom pluchea indica extract in various doses were administered. Temperature measurement was performed before the administration of DPT-HB vaccine, 2 hours after administration of the vaccine, as well as pn the 30th, 60th, 90th, and 120th minute after intervention. Result: There was a slight reduction in mean rectal temperature of Wistar rats administered with Pluchea indica Less. extract. Conclusion: Pluchea indica Less. extract has no antipyretic effect on rats. Keywords: Pluchea indica Less., Antipyretic effect, DPT-HB vaccine, Rat  Abstrak: Latar belakang: Salah satu tumbuhan asli Indonesia yang dikenal dan tersebar dengan luas pada beberapa daerah serta berpotensi untuk dikembangkan menjadi tanaman obat yaitu tanaman Beluntas (Pluchea indica (L.) Less.). Tujuan: Penelitian ini bertujuan untuk mengetahui ada tidaknya efek antipiretik ekstrak daun beluntas (Pluchea indica (L.) Less.) pada tikus Wistar (Rattus norvegicus) jantan. Metode: Penelitian ini menggunakan metode eksperimental. Hewan coba yang digunakan adalah tikus wistar jantan sebanyak 15 ekor dengan berat badan 150-200 gram. Hewan coba dibagi 5 kelompok yaitu kelompok kontrol negatif (aquades), kelompok kontrol positif (parasetamol), dan kelompok perlakuan yaitu pemberian ekstrak daun Beluntas. Pengukuran suhu dilakukan sebelum pemberian vaksin DPT-HB, 2 jam setelah pemberian DPT-HB, dan menit ke-30, 60, 90, 120 setelah pemberian perlakuan. Hasil: Suhu rata-rata rectal tikus yang diberikan ekstrak daun beluntas mengalami penurunan sedikit. Kesimpulan: Ekstrak daun beluntas tidak mempunyai efek antipiretik pada tikus. Kata Kunci : daun beluntas (Pluchea indica Less.), efek antipiretik, vaksin DPT-HB, tikus.

scholarly journals The Effect of Isotoma longiflora Leaves Extract to The Cornea Neovascularization of Wistar Rats Chemical Trauma Model

2020 ◽  
Vol 6 (2) ◽  
pp. 92
Author(s):  
Hasbi Maulana Arsyad ◽  
Cicih Komariah ◽  
Muhamad Hasan
Keyword(s):  
Wistar Rats ◽  
Design Method ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Post Test ◽  
Male Wistar Rats ◽  
Trauma Model ◽  
Post Hoc

Eye chemical trauma was an eye trauma caused by a substance with a pH<7 (acid) and pH>7 (alkali). Chemical trauma can be caused by pesticides. In Indonesia, 78.9% of farmers had eye complaints due to pesticides exposure. The occurrence of alkali chemical trauma of eye could cause cornea neovascularization (CNV). Alkali chemical trauma caused intense inflammation resulting a vascularization of the cornea that was supposed to be avascular. The purpose of this study was to find out the effect of Isotoma longiflora leaves extract the cornea neovascularization of wistar rats chemical trauma model. This research used true experimental design method with post-test only controlled group design. The sample of this study were 30 male wistar rats (Rattus norvegicus) divided into six groups, 1 positive control group (dexamethasone 0.1%), 1  negative control group (DMSO 0,1%), and 4 treatment groups of Isotoma longiflora leaves extract (0.25 mg/ml, 0.5 mg/ml, 1 mg/ml, 2 mg/ml). The result of Shapiro-Wilk and Levene's test showed that p>0.05 that means the data was normally distributed and has the same variant. One Way ANOVA test results obtained significant differences between groups on the 7th day (p=0.001). The result of Post Hoc LSD test showed that group P3 and P4 were significantly different to negative control, P1 and P2 groups. P3 and P4 groups with 1 mg/ml and 2 mg/ml Isotoma longiflora leaves extract could inhibit cornea neovascularization.

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