Study of Chlamydia trachomatis Infection in Infertile Females at a  Tertiary Care Hospital in Mumbai, India

Presence ofChlamydia trachomatisin endocervix was determined in 2466 women attending a tertiary care hospital in New Delhi, India over a period of 16 years, using a monoclonal-based direct immunofluorescence assay, tissue culture isolation, and a conventional PCR assay.Chlamydiaantigen could be detected in 391 out of 2466 (15.85%) of patients studied; in 27.27% women with PID, 16.74% women with cervicitis, 16.03% women with infertility, and 12.06% women with adverse pregnancy outcomes, respectively. There was a statistically significant decreasing trend inChlamydiaantigen positivity between the years 1994–1999 and 2000–2004; the apparent decline in antigen positivity between the years 2000–2004 and 2005–2010 was not statistically significant. Antigen detection assay detected equal number of positives as the PCR assay; tissue culture isolation demonstrated lower positivity. In a few representative specimens from cervicitis patients, genotyping was done using RFLP pattern analysis ofC. trachomatisMOMP gene amplified by PCR assay, all of these belonged toChlamydia trachomatisserovar E.

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Detection of Chlamydia Trachomatis Specific IgM Antibodies among Women of Reproductive Age Group Attending a Tertiary Care Hospital of South India

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2019.811.078 ◽

2019 ◽

Vol 8 (11) ◽

pp. 652-655

Author(s):

A. Neena

Keyword(s):

Chlamydia Trachomatis ◽

South India ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Reproductive Age ◽

Care Hospital ◽

Age Group ◽

Women Of Reproductive Age ◽

Igm Antibodies ◽

Reproductive Age Group

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Association of Genital Chlamydia trachomatis Infection with Female Infer-tility, Study in a Tertiary Care Hospital in Eastern India

The Open Microbiology Journal ◽

10.2174/1874285801509010110 ◽

2015 ◽

Vol 9 (1) ◽

pp. 110-116 ◽

Cited By ~ 6

Author(s):

Mallika Ghosh ◽

Subhadip Choudhuri ◽

Reena Ghosh Ray ◽

Basudev Bhattacharya ◽

Sujata Bhattacharya

Keyword(s):

Chlamydia Trachomatis ◽

Tertiary Care ◽

Elisa Test ◽

Care Hospital ◽

Serological Tests ◽

Chlamydia Trachomatis Infection ◽

Sexually Transmitted ◽

Medical College ◽

Significant Difference ◽

Iga Antibody

Background:Chlamydia trachomatisis recognized as one of the most common sexually transmitted pathogen in the world. 50-80% of infected females are asymptomatic. These untreated women are at risk of developing chronic sequelae leading to tubal pathology causing infertility. Infertility is defined as 1 year of unprotected intercourse without pregnancy. It may be primary or secondary.Aim:To find out the association of genitalChlamydia trachomatisinfection with female infertility.Materials and Methodology:This case control study has been carried out in collaboration with R. G. Kar Medical College and Institute of Post Graduate Medical Education & Research, India, between July 2012 and June 2013. 40 infertile and 40 pregnant women were enrolled by purposive sampling as per inclusion and exclusion criteria. ELISA test was performed to detect serum IgG and IgA antibody against recombinant analogs of MOMP and 3 different PCR assays were done targeting MOMP and rRNA DNA from DNA extracted from first void urine.Results:IgG seropositivity was significantly higher (15%vs0%,P=.0255) in cases than controls, though there was no significant difference in the proportion of IgA seropositivity among 2 groups (12.5%vs2.5%,P=0.2007). Out of 80 samples 2 samples showed the production of amplicons with R1 – R2 primers. Only 1 sample gave positive result with production of amplicons with all the 3 primers used (R1 – R2, CT0005 – CT06 and JM15 – JM16).Conclusion:PersistentC. trachomatisinfection must be recognized as a risk factor of infertility in this region of India. The low PCR positivity in FVU sample helps to conclude the diagnostic utility of serological tests in screening of infertile women.

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Genital Mycoplasma and Chlamydia trachomatis infections in patients with genital tract infections attending a tertiary care hospital of North India

Indian Journal of Pathology and Microbiology ◽

10.4103/0377-4929.182019 ◽

2016 ◽

Vol 59 (2) ◽

pp. 194 ◽

Cited By ~ 7

Author(s):

Benu Dhawan ◽

Karnika Saigal ◽

Jyoti Rawre ◽

Neena Khanna ◽

Rama Chaudhry

Keyword(s):

Chlamydia Trachomatis ◽

Genital Tract ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

North India ◽

Care Hospital ◽

Genital Tract Infections ◽

Tract Infections

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Association between Prior Chlamydia trachomatis Infection and Ectopic Pregnancy at a Tertiary Care Hospital in South Western Uganda

Obstetrics and Gynecology International ◽

10.1155/2018/4827353 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

Derrick Paul Mpiima ◽

George Wasswa Salongo ◽

Henry Lugobe ◽

Augustine Ssemujju ◽

Olivier Mumbere Mulisya ◽

...

Keyword(s):

Chlamydia Trachomatis ◽

Ectopic Pregnancy ◽

Bacterial Infections ◽

Strong Association ◽

Tertiary Care ◽

Care Hospital ◽

Chlamydia Trachomatis Infection ◽

Sexually Transmitted ◽

Tubal Factor ◽

Western Uganda

Background. Increase in the number of ectopic pregnancy is attributed to increase in the incidence of pelvic infections. Chlamydia trachomatis is responsible for most of the sexually transmitted bacterial infections. If undetected and untreated, the infection can ascend to the upper genital tract and cause pelvic inflammatory disease (PID) and related sequelae (ectopic pregnancy and tubal factor infertility). Objective. To determine the association between prior Chlamydia trachomatis infection and ectopic pregnancy at Mbarara Regional Referral Hospital (MRRH). Methods. This was an unmatched case-control study carried out at MRRH involving 25 cases and 76 controls. Serological evidence of prior chlamydial infection was determined by testing for the presence of Chlamydia immunoglobulin G antibodies in their blood. Logistic regression was used to determine the association between prior Chlamydia trachomatis infection and also the factors associated with ectopic pregnancy. The significant level of <0.05 was used. Results. Chlamydia antibodies were found in 60% of patients with ectopic pregnancy and 26.3% of the controls (p=0.002). The presence of Chlamydia antibodies was associated with a fourfold risk of ectopic pregnancy. Conclusion. There was a strong association between prior Chlamydia trachomatis infection and ectopic pregnancy.

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