scholarly journals Antimicrobial, anti-biofilm and anti-quorum sensing compounds from the bark of Ekebergia capensis

2020 ◽  
Vol 7 (9) ◽  
pp. 20-28
Author(s):  
Abu F. Meka ◽  
Yadessa M. Ayana ◽  
Teshome G. Biru
Keyword(s):  
Quorum Sensing ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antimicrobial Activities ◽  
Test Methods ◽  
Test Tube ◽  
Swarming Motility ◽  
Production Methods ◽  
Chemical Structures ◽  
Violacein Production

The main objective of this study was to isolate bioactive compounds from Ekebergia capensis for antimicrobial, anti-biofilm and anti-quorum sensing activity. The bark of E. capensis was extracted using hexane, ethyl acetate and methanol for 72 hours on maceration at room temperature. Antibacterial activity was evaluated against Staphylococcus aureus and antifungal activity against Candida albicans at 5 and 10 mg/mL concentrations. Anti-biofilm activity was tested using test tube and swarming motility test methods and anti-quorum sensing was evaluated using flask incubation test for violacein inhibition production methods. The results showed ethyl acetate extract of E. capensis and its two isolated compounds exhibited antimicrobial, anti-biofilm and anti-quorum sensing activity. Inhibition zones of antimicrobial activities of the extract against S. aureus and C. albicans ranged between 4-20 mm. 10 mg/mL extract showed inhibition of biofilm formation against S. aureus, anti-swarming activities against P. aeruginosa and S. aureus. Inhibition of violacein production against S. aureus, P. aeruginosa, and C. albicans were 72.6%, 67.7% and 69.3% respectively. The ethyl acetate extract, which showed antimicrobials, anti-biofilm, anti-quorum sensing activities, after silica gel column chromatography furnished two compounds namely; diisobutylphtalate and Sandropin B. The chemical structures of these compounds were done using spectroscopic methods including IR and NMR. The isolated compounds also showed strong antimicrobial, anti-biofilm, and anti-quorum sensing activity. This could be useful to manage infectious diseases caused by biofilm forming microorganisms.

scholarly journals CONTRIBUTE TO STUDY ON CHEMICAL CONSTITUENTS OF URENA LOBATA (L.) OF VIETNAM

2019 ◽  
Vol 57 (2) ◽  
pp. 162
Author(s):  
Quan Minh Pham ◽  
Hoai Van Thi Tran ◽  
Lam Tien Do ◽  
Phuong Lan Doan ◽  
Inh Thi Cam ◽  
...  
Keyword(s):  
Mental Disorders ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Tree Roots ◽  
Chemical Structures ◽  
Phytochemical Investigation ◽  
First Time

Urena lobata L. is used in Vietnamese traditional medicine for the treatment of several diseases. Tree roots are used to treat rheumatism, dysentery, poor digestion, flu, tonsils, malaria, asthma, goiter. Flowers are used to treat chickenpox, fever, and mental disorders. Branches, leaves or whole trees used to treat injuries bruises, rheumatism, mastitis, bites. Phytochemical investigation of the n-hexan and ethyl acetate extract of Urena lobata L. led to the isolation of β-sitosterol (1), β-sitosterol-3-O-β-D-glucopyranoside (2), a-acetylamino-phenylpropyl a-benzoylamino-phenylpropanoate (3), quercetin (4), and trans-tiliroside (5). Their chemical structures were determined by spectroscopic methods including MS, 1D, 2D NMR and comparing with those reported in previous papers. Two compounds 3, 5 were isolated for the first time from Urena lobata plant.

scholarly journals A preliminary study on the antimicrobial activities and gas chromatography-mass spectrometry (GC-MS) analysis of the ethyl acetate extract of Dennettia tripetala G. Baker seeds

Bio-Research ◽  
2020 ◽  
Vol 18 (1) ◽  
pp. 1094-1102
Author(s):  
UF Babaiwa ◽  
SO Eraga ◽  
EO Ojugo ◽  
O Erharuyi ◽  
JO Akerele
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Fatty Acid Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Traditional Use ◽  
Dennettia Tripetala

The study investigated the antimicrobial properties and the chemical composition of ethyl acetate extract of Dennettia tripetala (pepper fruit) seeds. Crude extract obtained by maceration of pulverized seeds in ethyl acetate was evaluated for antimicrobial activity against Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans and Aspergillus niger using standard agar-well diffusion method. GC-MS method was used to determine the chemical constituents of the extract. The extract was oily, yellowish-brown with a yield of 1.66 % and had activity against most of the test microorganisms, with inhibition zone diameters ranging between 10 to 25 mm. About 41 chemical constituents were present in the extract with formic acid methyl esters and fatty acids accounting for 57.23 and 18.49 % respectively. Ethyl acetate extract of Dennettia tripetala seeds possessed antimicrobial activity against bacteria but not fungi. The observed activity may be due to the presence of formic and fatty acid esters in the seed. The study further established a scientific proof for the traditional use of Dennettia tripetala seed extracts in treating microbial infections.  

scholarly journals In vitro Antibacterial Activity and Phytochemical Analysis of Nicotiana tabacum L. Extracted in Different Organic Solvents

2017 ◽  
Vol 11 (1) ◽  
pp. 352-359 ◽  
Author(s):  
Gemechu Ameya ◽  
Aseer Manilal ◽  
Behailu Merdekios
Keyword(s):  
Nicotiana Tabacum ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Organic Solvents ◽  
Ethyl Acetate Extract ◽  
Antimicrobial Activities ◽  
Modern Medicine ◽  
Phytochemical Analysis ◽  
Human Pathogens

Background: Controlling infectious disease using medicinal plants is the oldest healthcare known to mankind. Regardless of the enormous advances observed in modern medicine, medicinal plants are still playing vital roles. However, only a small proportion of medicinal plants are examined for bioactive compounds which may vary in different factors. This study aimed to evaluate phytochemical constituent and antimicrobial activities of Nicotiana tabacum L. extracted by different solvents against three set of bacteria. Methods: Nicotiana tabacum L. was collected from the Western Ethiopia and extracted in seven organic solvents. An in-vitro anti-bacterial activity of plant extracts was carried out by agar well diffusion assay against microbial type culture collection of human pathogens, clinical bacterial isolates, and biofilm forming bacteria. Gas Chromatographic and Mass Spectroscopic (GC-MS) analysis was used to determine the phytochemical constituents. Results: Antimicrobial activities of plant extract vary by extraction solvents; and ethyl acetate based extracts showed better antimicrobial activities. Of the experimental organisms, biofilm forming uropathogens were the most sensitive while clinical isolates were quite resistant. Analysis of the active ethyl acetate extract by GC-MS evinced a mixture of five volatile compounds; and Pyridine, 3-(1-methyl-2-pyrrolidinyl)-, (S) was the major compound detected. The overall results of the present study revealed that N. tabacum L extract has high antimicrobial activities against biofilm forming uropathogens. Conclusion: High antimicrobial activity was observed in ethyl acetate extract of N. tabacum against the biofilm forming bacteria whereas the clinically isolated bacteria were the most resistant group. The antibacterial property demonstrated could be due to Pyridine, 3-(1-methyl-2-pyrrolidinyl)-(S) with a broad spectrum of activity.

scholarly journals Extraction, Evaluation and Structure Elucidation of Bioactive Metabolites of Lactobacillus helveticus CNRZ 32

2020 ◽  
Vol 11 (1) ◽  
pp. 7677-7688
Keyword(s):  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Structure Elucidation ◽  
Octadecadienoic Acid ◽  
Ethyl Acetate Extract ◽  
Chitosan Nanoparticles ◽  
Antimicrobial Activities ◽  
Lactobacillus Helveticus ◽  
Bioactive Metabolites ◽  
Metabolic Profiles

There is no previous work that utilizes the multi-solvent extraction and structure elucidation of Lactobacillus helveticus cell-free supernatant (CFS). In this study, the CFS of Lb. helveticus CNRZ 32 was extracted by ethyl acetate, diethyl ether, dichloromethane, and n-hexane solvents. The extracts of considerable antimicrobial activities were characterized through GC/MS clarify metabolic profiles, TLC for compounds separation, and bio-autography to determine the number and Rf of effective compounds. Ethyl acetate extract possessed the strongest effect on all tested pathogens with inhibition diameter reached 38 mm in the case of Staphylococcus sciuri, while Diethyl ether and Dichloromethane extracts came secondly. The extract of Ethyl acetate mainly included butyl lactate with area % (59.45), while 9,12-Octadecadienoic acid (Z,Z)-, methyl ester and different health beneficial compounds were identified in both Diethyl ether and Dichloromethane extracts. Due to the strong synergism among Chitosan Nanoparticles and different extracts, the MIC values were lowered by about 20 – 50%.

scholarly journals Evaluation of the Antioxidant and Antimicrobial Activities of Ethyl Acetate Extract of Saccharomyces cerevisiae

2021 ◽  
Vol 59 (2) ◽  
Author(s):  
Essam A. Makky ◽  
Manaf AlMatar ◽  
Mahmood H. Mahmood ◽  
Ooi Wei Ting ◽  
Wong Zi Qi
Keyword(s):  
Staphylococcus Aureus ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Ethyl Acetate Extract ◽  
Antimicrobial Activities ◽  
New Drugs ◽  
Synthetic Antioxidants ◽  
Cutibacterium Acnes ◽  
Antioxidant And Antimicrobial Activities

Research background. Antioxidants are described as important compounds that are present at low concentrations to inhibit oxidation processes. Due to the side effects of synthetic antioxidants, research interest has increased considerably towards finding natural sources of antioxidants that can replace synthetic antioxidants. The emergence and spread of antibiotic resistance require the development of new drugs or some potential sources of novel medicine. This work aims to extract the secondary metabolites of S. cerevisiae using ethyl acetate as a solvent and to determine the antioxidant and antimicrobial activities of these extracted metabolites. Experimental approach. The antioxidant activities of the secondary metabolites of S. cerevisiae were determined using DPPH, ABTS, and FRAP assays. Furthermore, the antimicrobial potential of the ethyl acetate extract of S. cerevisiae in treating Cutibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis was assessed. Results and conclusion. Five out of 13 of the extracted secondary metabolites were identified as antioxidants. The antioxidant activity of the S. cerevisiae extract exhibited relatively high IC50 of 455.2689 μg/mL and 294.51 μg/mL for DPPH and ABTS respectively while the FRAP value was obtained as 44.4004 μg AAE/mL. Moreover, the extracts presented a significant antibacterial activity (p<0.05) against Staphylococcus aureus and Staphylococcus epidermidis at the concentrations of 100 mg/mL and 200 mg/mL, respectively. However, no inhibitory effect was observed against Cutibacterium acnes as the extract was only effective against Cutibacterium acnes at the concentrations of 300 mg/mL and 400 mg/mL (inhibition zones ranging from 9.0±0 to 9.333±0.577) respectively (p<0.05). Staphylococcus aureus was highly sensitive to the extract, with a MIC value of 18.75 mg/mL. Novelty and scientific contribution. This report confirmed the efficacy of the secondary metabolites of S. cerevisiae as a natural source of antioxidants and antimicrobials and suggest the possibility of employing them in drugs for the treatment of infectious diseases caused by the tested microorganisms.

scholarly journals Evaluation of Antifibrin, Antioxidant and Antimicrobial Activities of Betel Leaves (Piper betel L.)

2019 ◽  
pp. 1-9
Author(s):  
Kollipara Venkata Manideep ◽  
Pattipati Anusha ◽  
Mulpuri Ramesh Babu ◽  
B. R. S. S. Srinivas Gupta ◽  
M. Swathi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antimicrobial Activities ◽  
Fungal Species ◽  
Clot Lysis ◽  
Local Market ◽  
The Family ◽  
Solvent Systems ◽  
Layer Chromatography ◽  
Rotary Vacuum

Piper betel L. belongs to the family Piperaceae. It has been an important medicinal agent since ages in various traditional and folk systems of medicine. Leaves obtained from the local market were shade dried and powdered. Different solvents were used based on polarity to extract phytochemicals from this powder using a Soxhlet extractor and separated using rotary vacuum evaporator. Thin layer chromatography was run using different solvent systems in different ratios for identifying essential compounds of Piper betel and for standardizing the ratios at which better resolution of compounds taken place. Antimicrobial activities were tested on twelve bacterial and three fungal species. Also, anti fibrin activity was tested on erythrocytes by using the extracts obtained by the plant. The zone of inhibitions formed due to the anti microbial activity were measured and found that mixtures of ethyl acetate and ethanol were effective. The percentage of clot lysis was found to be appreciable for ethyl acetate extract of the Piper leaves.

scholarly journals Studies on comparative antimicrobial activities of Aerva lanata and Momordica charantia leaf extracts

2020 ◽  
pp. 561-564
Author(s):  
Nagula Shilpika ◽  
Byagari Teena Keerthana ◽  
Banka Srinivas ◽  
Gampa Tulja Rani ◽  
Ganga Maithili
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antimicrobial Activities ◽  
Momordica Charantia ◽  
Leaf Extracts ◽  
Plate Method ◽  
Methanolic Extracts ◽  
Aerva Lanata

Screening and comparison of antimicrobial action of leaf extract of Aerva lanata and Momordica charantia. Ethyl acetate and methanolic extracts of leaves of plants were screened for antimicrobial activity using the cup plate method and the spread plate method against gram positive and gram negative reference organisms (Bacillus substilis and Escherichia coli).The standard antibacterial agent used for reference is Choramphenicol and the results were calculated as zone of inhibition. Methanolic extract showed comparatively broader and better antimicrobial spectrum than ethyl acetate extract in selected plants. Plant extracts showed dose dependent action, results were similar to the action of the standard Choramphenicol. Extracts of Aerva lanata and Momordica charantia demonstrated antimicrobial activity on tested microorganisms. Methanolic extracts showed higher antimicrobial potential than ethyl acetate extract. Aerva lanata extracts showed better response than Momordica charantia extracts in the cup plate method antibacterial activity with Bacillus substilis and Escherichia coli.

scholarly journals Chemical Composition, Anti-Quorum Sensing, Enzyme Inhibitory, and Antioxidant Properties of Phenolic Extracts of Clinopodium nepeta L. Kuntze

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1955
Author(s):  
Hatem Beddiar ◽  
Sameh Boudiba ◽  
Merzoug Benahmed ◽  
Alfred Ngenge Tamfu ◽  
Özgür Ceylan ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Frap Assay ◽  
Swarming Motility ◽  
Dependent Inhibition ◽  
Test Concentration ◽  
Phenolic Profiles ◽  
Violacein Production ◽  
Phenolic Extracts

Phenolic extracts of Clinopodium nepeta were prepared and their preliminary phenolic profiles determined using HPLC-DAD with 26 phenolic standards. Apigenin (21.75 ± 0.41 µg/g), myricetin (72.58 ± 0.57 µg/g), and rosmarinic acid (88.51 ± 0.55 µg/g) were the most abundant compounds in DCM (dichloromethane), AcOEt (ethyl acetate), and BuOH (butanol) extracts, respectively. The DCM and AcOEt extracts inhibited quorum-sensing mediated violacein production by C. violaceum CV12472. Anti-quorum-sensing zones on C. violaceum CV026 at MIC (minimal inhibitory concentration) were 10.3 ± 0.8 mm for DCM extract and 12.0 ± 0.5 mm for AcOEt extract. Extracts showed concentration-dependent inhibition of swarming motility on flagellated P. aeruginosa PA01 and at the highest test concentration of 100 μg/mL, AcOEt (35.42 ± 1.00%) extract displayed the best activity. FRAP assay indicated that the BuOH extract (A0.50 = 17.42 ± 0.25 µg/mL) was more active than standard α-tocopherol (A0.50 = 34.93 ± 2.38 µg/mL). BuOH extract was more active than other extracts except in the ABTS●+, where the DCM extract was most active. This antioxidant activity could be attributed to the phenolic compounds detected. C. nepeta extracts showed moderate inhibition on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, and α-amylase. The results indicate that C. nepeta is a potent source of natural antioxidants that could be used in managing microbial resistance and Alzheimer′s disease.

scholarly journals Investigation of bioative compounds from the ethyl acetate extract of Euphorbia hirta Linn

2018 ◽  
Vol 1 (T5) ◽  
pp. 95-101
Author(s):  
Ngan Thi Kim Tran ◽  
Ly Thi Le ◽  
Nhi Thi Y Nguyen ◽  
Minh Thi Tran ◽  
Quan Le Tran
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Gastrointestinal Disorders ◽  
Published Data ◽  
Spectroscopic Methods ◽  
Asian Countries ◽  
Euphorbia Hirta ◽  
Chemical Structures ◽  
The Past

Euphorbia hirta Linn. (co sua la lon in Vietnamese) belongs to Euphorbiaceae family, is a group of small prostrate herbaceous annual weed in Vietnam. It is abundant in waste places and open grasslands and distributes in most Asian countries. E. hirta is traditionally used in the treatment of gastrointestinal disorders, bronchial and respiratory. The aqueous extract exhibits anxiolytic, analgesic, antipyretic, and anti-inflammatory activities. Strong anti-diabetic activity of Euphorbiaceae family in general and E. hirta in particular was reported in the past investigations. E. hirta has been studied by various investigations and several active constituents have been isolated and identified successfully. Most of those compounds have strong biological activities. At the first step in the processing of the isolation of bioactive compounds from the ethyl acetate extract, we isolated four purified compounds, including methyl gallate (1), quercetin (2), myrecitin (3), and quercitrin (4). The chemical structures of those compounds were elucidated by spectroscopic methods and compared with published data in the literature.

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