Cytotoxic and antimicrobial activities of ethyl acetate extract of mangrove plant Scyphiphora hydrophyllacea C. F. Gaertn— Associated fungi

The study investigated the antimicrobial properties and the chemical composition of ethyl acetate extract of Dennettia tripetala (pepper fruit) seeds. Crude extract obtained by maceration of pulverized seeds in ethyl acetate was evaluated for antimicrobial activity against Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans and Aspergillus niger using standard agar-well diffusion method. GC-MS method was used to determine the chemical constituents of the extract. The extract was oily, yellowish-brown with a yield of 1.66 % and had activity against most of the test microorganisms, with inhibition zone diameters ranging between 10 to 25 mm. About 41 chemical constituents were present in the extract with formic acid methyl esters and fatty acids accounting for 57.23 and 18.49 % respectively. Ethyl acetate extract of Dennettia tripetala seeds possessed antimicrobial activity against bacteria but not fungi. The observed activity may be due to the presence of formic and fatty acid esters in the seed. The study further established a scientific proof for the traditional use of Dennettia tripetala seed extracts in treating microbial infections.

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In vitro Antibacterial Activity and Phytochemical Analysis of Nicotiana tabacum L. Extracted in Different Organic Solvents

The Open Microbiology Journal ◽

10.2174/1874285801711010352 ◽

2017 ◽

Vol 11 (1) ◽

pp. 352-359 ◽

Cited By ~ 4

Author(s):

Gemechu Ameya ◽

Aseer Manilal ◽

Behailu Merdekios

Keyword(s):

Nicotiana Tabacum ◽

Medicinal Plants ◽

Ethyl Acetate ◽

Organic Solvents ◽

Ethyl Acetate Extract ◽

Antimicrobial Activities ◽

Modern Medicine ◽

Phytochemical Analysis ◽

Human Pathogens

Background: Controlling infectious disease using medicinal plants is the oldest healthcare known to mankind. Regardless of the enormous advances observed in modern medicine, medicinal plants are still playing vital roles. However, only a small proportion of medicinal plants are examined for bioactive compounds which may vary in different factors. This study aimed to evaluate phytochemical constituent and antimicrobial activities of Nicotiana tabacum L. extracted by different solvents against three set of bacteria. Methods: Nicotiana tabacum L. was collected from the Western Ethiopia and extracted in seven organic solvents. An in-vitro anti-bacterial activity of plant extracts was carried out by agar well diffusion assay against microbial type culture collection of human pathogens, clinical bacterial isolates, and biofilm forming bacteria. Gas Chromatographic and Mass Spectroscopic (GC-MS) analysis was used to determine the phytochemical constituents. Results: Antimicrobial activities of plant extract vary by extraction solvents; and ethyl acetate based extracts showed better antimicrobial activities. Of the experimental organisms, biofilm forming uropathogens were the most sensitive while clinical isolates were quite resistant. Analysis of the active ethyl acetate extract by GC-MS evinced a mixture of five volatile compounds; and Pyridine, 3-(1-methyl-2-pyrrolidinyl)-, (S) was the major compound detected. The overall results of the present study revealed that N. tabacum L extract has high antimicrobial activities against biofilm forming uropathogens. Conclusion: High antimicrobial activity was observed in ethyl acetate extract of N. tabacum against the biofilm forming bacteria whereas the clinically isolated bacteria were the most resistant group. The antibacterial property demonstrated could be due to Pyridine, 3-(1-methyl-2-pyrrolidinyl)-(S) with a broad spectrum of activity.

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Extraction, Evaluation and Structure Elucidation of Bioactive Metabolites of Lactobacillus helveticus CNRZ 32

Biointerface Research in Applied Chemistry ◽

10.33263/briac111.76777688 ◽

2020 ◽

Vol 11 (1) ◽

pp. 7677-7688

Keyword(s):

Ethyl Acetate ◽

Diethyl Ether ◽

Structure Elucidation ◽

Octadecadienoic Acid ◽

Ethyl Acetate Extract ◽

Chitosan Nanoparticles ◽

Antimicrobial Activities ◽

Lactobacillus Helveticus ◽

Bioactive Metabolites ◽

Metabolic Profiles

There is no previous work that utilizes the multi-solvent extraction and structure elucidation of Lactobacillus helveticus cell-free supernatant (CFS). In this study, the CFS of Lb. helveticus CNRZ 32 was extracted by ethyl acetate, diethyl ether, dichloromethane, and n-hexane solvents. The extracts of considerable antimicrobial activities were characterized through GC/MS clarify metabolic profiles, TLC for compounds separation, and bio-autography to determine the number and Rf of effective compounds. Ethyl acetate extract possessed the strongest effect on all tested pathogens with inhibition diameter reached 38 mm in the case of Staphylococcus sciuri, while Diethyl ether and Dichloromethane extracts came secondly. The extract of Ethyl acetate mainly included butyl lactate with area % (59.45), while 9,12-Octadecadienoic acid (Z,Z)-, methyl ester and different health beneficial compounds were identified in both Diethyl ether and Dichloromethane extracts. Due to the strong synergism among Chitosan Nanoparticles and different extracts, the MIC values were lowered by about 20 – 50%.

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Evaluation of the Antioxidant and Antimicrobial Activities of Ethyl Acetate Extract of Saccharomyces cerevisiae

Food Technology and Biotechnology ◽

10.17113/ftb.59.02.21.6658 ◽

2021 ◽

Vol 59 (2) ◽

Author(s):

Essam A. Makky ◽

Manaf AlMatar ◽

Mahmood H. Mahmood ◽

Ooi Wei Ting ◽

Wong Zi Qi

Keyword(s):

Staphylococcus Aureus ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Staphylococcus Epidermidis ◽

Ethyl Acetate Extract ◽

Antimicrobial Activities ◽

New Drugs ◽

Synthetic Antioxidants ◽

Cutibacterium Acnes ◽

Antioxidant And Antimicrobial Activities

Research background. Antioxidants are described as important compounds that are present at low concentrations to inhibit oxidation processes. Due to the side effects of synthetic antioxidants, research interest has increased considerably towards finding natural sources of antioxidants that can replace synthetic antioxidants. The emergence and spread of antibiotic resistance require the development of new drugs or some potential sources of novel medicine. This work aims to extract the secondary metabolites of S. cerevisiae using ethyl acetate as a solvent and to determine the antioxidant and antimicrobial activities of these extracted metabolites. Experimental approach. The antioxidant activities of the secondary metabolites of S. cerevisiae were determined using DPPH, ABTS, and FRAP assays. Furthermore, the antimicrobial potential of the ethyl acetate extract of S. cerevisiae in treating Cutibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis was assessed. Results and conclusion. Five out of 13 of the extracted secondary metabolites were identified as antioxidants. The antioxidant activity of the S. cerevisiae extract exhibited relatively high IC50 of 455.2689 μg/mL and 294.51 μg/mL for DPPH and ABTS respectively while the FRAP value was obtained as 44.4004 μg AAE/mL. Moreover, the extracts presented a significant antibacterial activity (p<0.05) against Staphylococcus aureus and Staphylococcus epidermidis at the concentrations of 100 mg/mL and 200 mg/mL, respectively. However, no inhibitory effect was observed against Cutibacterium acnes as the extract was only effective against Cutibacterium acnes at the concentrations of 300 mg/mL and 400 mg/mL (inhibition zones ranging from 9.0±0 to 9.333±0.577) respectively (p<0.05). Staphylococcus aureus was highly sensitive to the extract, with a MIC value of 18.75 mg/mL. Novelty and scientific contribution. This report confirmed the efficacy of the secondary metabolites of S. cerevisiae as a natural source of antioxidants and antimicrobials and suggest the possibility of employing them in drugs for the treatment of infectious diseases caused by the tested microorganisms.

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Evaluation of Antifibrin, Antioxidant and Antimicrobial Activities of Betel Leaves (Piper betel L.)

European Journal of Medicinal Plants ◽

10.9734/ejmp/2019/v27i130105 ◽

2019 ◽

pp. 1-9

Author(s):

Kollipara Venkata Manideep ◽

Pattipati Anusha ◽

Mulpuri Ramesh Babu ◽

B. R. S. S. Srinivas Gupta ◽

M. Swathi ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antimicrobial Activities ◽

Fungal Species ◽

Clot Lysis ◽

Local Market ◽

The Family ◽

Solvent Systems ◽

Layer Chromatography ◽

Rotary Vacuum

Piper betel L. belongs to the family Piperaceae. It has been an important medicinal agent since ages in various traditional and folk systems of medicine. Leaves obtained from the local market were shade dried and powdered. Different solvents were used based on polarity to extract phytochemicals from this powder using a Soxhlet extractor and separated using rotary vacuum evaporator. Thin layer chromatography was run using different solvent systems in different ratios for identifying essential compounds of Piper betel and for standardizing the ratios at which better resolution of compounds taken place. Antimicrobial activities were tested on twelve bacterial and three fungal species. Also, anti fibrin activity was tested on erythrocytes by using the extracts obtained by the plant. The zone of inhibitions formed due to the anti microbial activity were measured and found that mixtures of ethyl acetate and ethanol were effective. The percentage of clot lysis was found to be appreciable for ethyl acetate extract of the Piper leaves.

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Studies on comparative antimicrobial activities of Aerva lanata and Momordica charantia leaf extracts

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2020.120519 ◽

2020 ◽

pp. 561-564

Author(s):

Nagula Shilpika ◽

Byagari Teena Keerthana ◽

Banka Srinivas ◽

Gampa Tulja Rani ◽

Ganga Maithili

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antimicrobial Activities ◽

Momordica Charantia ◽

Leaf Extracts ◽

Plate Method ◽

Methanolic Extracts ◽

Aerva Lanata

Screening and comparison of antimicrobial action of leaf extract of Aerva lanata and Momordica charantia. Ethyl acetate and methanolic extracts of leaves of plants were screened for antimicrobial activity using the cup plate method and the spread plate method against gram positive and gram negative reference organisms (Bacillus substilis and Escherichia coli).The standard antibacterial agent used for reference is Choramphenicol and the results were calculated as zone of inhibition. Methanolic extract showed comparatively broader and better antimicrobial spectrum than ethyl acetate extract in selected plants. Plant extracts showed dose dependent action, results were similar to the action of the standard Choramphenicol. Extracts of Aerva lanata and Momordica charantia demonstrated antimicrobial activity on tested microorganisms. Methanolic extracts showed higher antimicrobial potential than ethyl acetate extract. Aerva lanata extracts showed better response than Momordica charantia extracts in the cup plate method antibacterial activity with Bacillus substilis and Escherichia coli.

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Antimicrobial, anti-biofilm and anti-quorum sensing compounds from the bark of Ekebergia capensis

International Journal of Current Research in Biosciences and Plant Biology ◽

10.20546/ijcrbp.2020.709.003 ◽

2020 ◽

Vol 7 (9) ◽

pp. 20-28

Author(s):

Abu F. Meka ◽

Yadessa M. Ayana ◽

Teshome G. Biru

Keyword(s):

Quorum Sensing ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antimicrobial Activities ◽

Test Methods ◽

Test Tube ◽

Swarming Motility ◽

Production Methods ◽

Chemical Structures ◽

Violacein Production

The main objective of this study was to isolate bioactive compounds from Ekebergia capensis for antimicrobial, anti-biofilm and anti-quorum sensing activity. The bark of E. capensis was extracted using hexane, ethyl acetate and methanol for 72 hours on maceration at room temperature. Antibacterial activity was evaluated against Staphylococcus aureus and antifungal activity against Candida albicans at 5 and 10 mg/mL concentrations. Anti-biofilm activity was tested using test tube and swarming motility test methods and anti-quorum sensing was evaluated using flask incubation test for violacein inhibition production methods. The results showed ethyl acetate extract of E. capensis and its two isolated compounds exhibited antimicrobial, anti-biofilm and anti-quorum sensing activity. Inhibition zones of antimicrobial activities of the extract against S. aureus and C. albicans ranged between 4-20 mm. 10 mg/mL extract showed inhibition of biofilm formation against S. aureus, anti-swarming activities against P. aeruginosa and S. aureus. Inhibition of violacein production against S. aureus, P. aeruginosa, and C. albicans were 72.6%, 67.7% and 69.3% respectively. The ethyl acetate extract, which showed antimicrobials, anti-biofilm, anti-quorum sensing activities, after silica gel column chromatography furnished two compounds namely; diisobutylphtalate and Sandropin B. The chemical structures of these compounds were done using spectroscopic methods including IR and NMR. The isolated compounds also showed strong antimicrobial, anti-biofilm, and anti-quorum sensing activity. This could be useful to manage infectious diseases caused by biofilm forming microorganisms.

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Antimicrobial Activity of Allium chinense G. Don.

Current Biochemistry ◽

10.29244/cb.2.3.129-138 ◽

2017 ◽

Vol 2 (3) ◽

pp. 129-138

Author(s):

Frans Grovy Naibaho ◽

Maria Bintang ◽

Fachriyan Hasmi Pasaribu

Keyword(s):

Antimicrobial Activity ◽

Ethyl Acetate ◽

Microbial Growth ◽

Inhibitory Concentration ◽

Ethyl Acetate Extract ◽

Salmonella Typhi ◽

Antimicrobial Activities ◽

Biologically Active ◽

E Coli ◽

Allium Chinense

This study aims to analyze antimicrobial activity of Allium chinense G. Don extract against Eschericia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis and Candida albicans, and to examine the active compounds. Allium chinense G. Don was extracted using maseration method and treated with ethanol 70% (v/v), ethanol 96% (v/v), ethyl acetate, n-hexane, and aquadest. Antimicrobial activity assay was conducted using agar difusion method and compounds analysis using Gas chromatography-massspectrometry (GC-MS). Antimicrobial activities assay showed that all extracts could inhibit microbial growth. Ethyl acetate extract has the highest antimicrobial activity against all the microbial test. Minimum inhibitory concentration (MIC) of ethyl acetate extract against C. albicans, B. subtilis, E. coli, S. aureus, S. typhi was 25, 100, 250, 250, 1000 mg ml-1 respectively. As many as 25 compounds were derived from GC-MS analysis and most of them were known as the antimicrobial compounds. This study revealed that Allium chinense G. Don contains biologically active compunds as antimicrobial agent particularly anti Candida.

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Evaluation of Antioxidant, Anti-inflammatory and Antimicrobial Activities of the Leaf Extracts of Luffa cylindrica

Journal of Health Sciences ◽

10.17532/jhsci.2018.423 ◽

2018 ◽

Vol 8 (2) ◽

pp. 101-109

Author(s):

Felix A. Onyegbule ◽

Chukwudozie I. Okoye ◽

Chidimma R. Chukwunwejim ◽

Blessing O. Umeokoli ◽

Peter M. Eze

Keyword(s):

Ethyl Acetate ◽

Leaf Extract ◽

Ethyl Acetate Extract ◽

Antimicrobial Properties ◽

Ethanol Extract ◽

Antimicrobial Activities ◽

Luffa Cylindrica ◽

Leaf Extracts ◽

Anti Inflammatory ◽

Ethyl Acetate Extracts

Introduction: Luffa cylindrica have been used traditionally in the treatment and management of several disease conditions. This study aimed to evaluate the antioxidant, anti-inflammatory and antimicrobial properties of leaf extracts of the plant, and identifying some of its phytoconstituents. Methods: The crude ethanol and ethyl acetate extracts were evaluated for antioxidant and anti-inflammatory activities using the 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) and paw-fluid displacement methods, respectively. The extracts were tested for antimicrobial activity using the agar well diffusion and agar dilution methods. The ethyl acetate leaf extract of the plant was further subjected to high-performance liquid chromatography-diode-array detection (HPLC-DAD) analysis for the identification of the bioactive compounds. Results: The ethanol and ethyl acetate extracts of L. cylindrica showed average antioxidant properties at 100 µg/mL, with inhibitions of 53.31% and 54.73% respectively. The ethanol extract displayed significant anti-inflammatory activity at 50 mg/Kg with an inhibition of 31.1% compared to 39.7% recorded for the control (diclofenac). The ethyl acetate extract produced an inhibition of 15%. In the antimicrobial evaluation, the ethanol and ethyl acetate extracts showed moderate antibacterial activity against S. aureus, S. Typhi and B. subtilis. The ethyl acetate extract exhibited considerable antimicrobial activity against the test isolates compared to the ethanol extract. HPLC-DAD analysis of the ethyl acetate extract suggested the presence of two flavonoid compounds - luteolin and apigenin as key components of the leaf extract of L. cylindrica. These compounds are known to possess anti-inflammatory, antioxidant and antimicrobial activities. Conclusions: The results of this study showed that the leaf extracts of L. cylindrica possess antioxidant, anti-inflammatory and antimicrobial properties.

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The Effect Of Mangrove Leaf Extract Dosage Sonneratia Alba On Hela Cell Viability

Journal of Stem Cell Research and Tissue Engineering ◽

10.20473/jscrte.v5i1.29383 ◽

2021 ◽

Vol 5 (1) ◽

pp. 30

Author(s):

Fitria Devi Suryaningrum

Keyword(s):

Hela Cell ◽

Cell Viability ◽

Ethyl Acetate ◽

Leaf Extract ◽

Ethyl Acetate Extract ◽

Reproductive Organs ◽

Mangrove Plant ◽

Ic50 Value ◽

Sonneratia Alba ◽

Leaf Powder

Cervical cancer is caused due to infection from the Human Papilloma Virus (HPV) which attacks the sexually active female reproductive organs. Treatment is carried out alternatively using natural ingredients such as mangroves. Sonneratia alba is a type of mangrove plant that has been used in alternative medicine because of its potential as an anticancer. This study aims to determine the effect of Sonneratia alba mangrove extract on heLa cell viability. The Sonneratia alba leaf powder was extracted using stratified maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 3.59 ppm, this means that the ethyl acetate extract has toxic properties. Phytochemical test results of Sonneratia alba leaf extract contain alkaloid compounds, steroids / triterpenoids, and tannins. The results of the test yield extract were 12.60%, extract water content was 21.24%, and total phenol was 504.08 mg / g GAE Test The results of the LC-MS test resulted in the suspicion of compounds including diosmetin, caffeine, and turmeron. The ethyl acetate extract of Sonneratia alba leaves was cytotoxic against heLa cell viability with the resulting IC50 value of 478.630 µg / mL

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