scholarly journals Analysis of genetic diversity among different Rose (Rosa) genotypes using morphological and molecular markers (ISSR)

2021 ◽  
Vol 8 (5) ◽  
pp. 14-23
Author(s):  
Ravisha Raina ◽  
Anita Grewal
Keyword(s):  
Similarity Coefficient ◽  
Morphological Parameters ◽  
Woody Perennial ◽  
Ctab Concentration ◽  
The Family ◽  
Average Similarity ◽  
Ctab Method ◽  
Flower Diameter ◽  
Principle Coordinate Analysis ◽  
Issr Primers

Rose is a woody perennial of the genus Rosa within the family Rosaceae. In this study, phylogenetic relationship among seven different genotypes of Rose (Rosa) was determined using ISSR primers and morphological parameters. DNA was isolated using CTAB method with 0.5% of CTAB concentration. Out of 50 ISSR primers, 16 primers gave reproducible banding pattern with all the seven genotypes of Rose while others failed to produce polymorphism. A total of 108 bands were obtained with an average polymorphism of 54.69% and an average of 9.06 bands per primer. The average similarity was found to be 0.69 among all the seven genotypes using Jaccard’s similarity coefficient. On basis of polymorphism cluster analysis divided them into two major groups separating one genotype (light pink) from rest of the six genotypes and principle coordinate analysis confirmed the results. A parallel correlation was found between the morphological parameter (flower diameter) and the results of ISSR analysis as both of them divided the seven genotypes into two main groups.

scholarly journals EVALUATION OF GENETIC DIVERSITY IN CACAO COLLECTED FROM KOLAKA, SOUTHEAST SULAWESI, USING SSR MARKERS

2016 ◽  
Vol 16 (2) ◽  
pp. 71
Author(s):  
Rubiyo Rubiyo ◽  
Nur Kholilatul Izzah ◽  
Indah Sulistiyorini ◽  
Cici Tresniawati
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Similarity Coefficient ◽  
Breeding Program ◽  
New Variety ◽  
Average Value ◽  
The Third ◽  
Ctab Method

Kolaka, which is located in Southeast Sulawesi, has long been known as one of cacao production centers in Indonesia. Therefore, many different cacao germplasms can be found in this region. The study aimed to evaluate genetic diversity and relationships of 12 cacao genotypes collected from Kolaka. Genomic DNA was extracted by using a modified CTAB method. Meanwhile, genetic diversity was analyzed based on 16 SSR markers, which then separated by 6% non-denaturing polyacryl-amide gel electrophoresis. The result showed that all of those markers, 14 markers exhibited polymorphism and subsequently used for data analysis using NTSYS and PowerMarker program. About 70 different alleles were generated from 12 cacao genotypes analyzed with an average of 5 alleles per locus. Average value of polymorphism information content (PIC) resulted in this study was 0.59. The cluster analysis using UPGMA method based on the genetic similarity coefficient revealed that all cacao genotypes were separated into three major groups. The first group consisted of five cacao genotypes, the second one held four cacao genotypes, whereas the third group contained three genotypes. This result indicates that three genotypes that clustered separately from the others could be used as a good clonal candidate for cacao breeding program. The information resulted from this present study would be useful for future cacao breeding program, especially in efforts to release a new variety.

Genetic variability and population structure of some Iranian Salvia limbata C. A. Mey. populations

2020 ◽  
Vol 29 ◽  
pp. 56-65 ◽  
Author(s):  
Seyed M. Talebi ◽  
Alex V. Matsyura
Keyword(s):  
Gene Flow ◽  
Genetic Differentiation ◽  
Genetic Divergence ◽  
Modern Medicine ◽  
Variance Test ◽  
Adaptive Mutations ◽  
Ctab Method ◽  
Research Population ◽  
Nj Tree ◽  
Issr Primers

Salvia limbata is one of the aromatic herbs of Lamiaceae that naturally grows in different regions of Iran. This species has several usages in folk and modern medicine. In the current research, population genetic divergence and structure were evaluated in eight Iranian populations of this species. Nuclear genomes were extracted using CTAB method and amplified by ten ISSR primers. Parameters of genetic divergence changed among and within the examined populations. Percentage of among populations polymorphism was higher than within population ones in most of studied populations. It was supported by results of Analysis of Molecular Variance test, which revealed the large part of significant genetic variation belonged to among populations rather than within populations. In this regard, individuals of most populations were close together, while according to UPGMA tree, the studied populations were clustered into seven groups. STRUCTURE analysis and NJ tree produced similar results. The low rate of gene flow (Lm) and highest value of genetic differentiation (GST) confirmed the high genetic differentiation of the studied populations. Popart analysis revealed the occurrence of several mutations in order to adapt populations with environmental conditions. The occurrence of adaptive mutations and gene flow disruption due to habitat separation seems to differentiate the populations.

scholarly journals Universal Patterns in Color-Emotion Associations Are Further Shaped by Linguistic and Geographic Proximity

2020 ◽  
Vol 31 (10) ◽  
pp. 1245-1260
Author(s):  
Domicele Jonauskaite ◽  
Ahmad Abu-Akel ◽  
Nele Dael ◽  
Daniel Oberfeld ◽  
Ahmed M. Abdel-Khalek ◽  
...  
Keyword(s):  
Learning Algorithm ◽  
Cognitive Architecture ◽  
Well Being ◽  
Similarity Coefficient ◽  
Geographic Proximity ◽  
Native Languages ◽  
Pattern Similarity ◽  
Average Similarity ◽  
Color Emotion ◽  
Emotion Concepts

Many of us “see red,” “feel blue,” or “turn green with envy.” Are such color-emotion associations fundamental to our shared cognitive architecture, or are they cultural creations learned through our languages and traditions? To answer these questions, we tested emotional associations of colors in 4,598 participants from 30 nations speaking 22 native languages. Participants associated 20 emotion concepts with 12 color terms. Pattern-similarity analyses revealed universal color-emotion associations (average similarity coefficient r = .88). However, local differences were also apparent. A machine-learning algorithm revealed that nation predicted color-emotion associations above and beyond those observed universally. Similarity was greater when nations were linguistically or geographically close. This study highlights robust universal color-emotion associations, further modulated by linguistic and geographic factors. These results pose further theoretical and empirical questions about the affective properties of color and may inform practice in applied domains, such as well-being and design.

scholarly journals Strong genetic differentiation of the Paracaryum species (Boraginaceae) detected by inter-simple sequence repeats (ISSR)

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 883-894
Author(s):  
Xinxin Zhang ◽  
Abdul Shakoor
Keyword(s):  
Genetic Parameters ◽  
Issr Markers ◽  
Genetic Distances ◽  
Similarity Coefficient ◽  
Descriptive Statistics ◽  
Jaccard Similarity ◽  
Inter Simple Sequence Repeats ◽  
The Family ◽  
Polymorphic Bands ◽  
Simple Sequence

Species identification is fundamentally important within the fields of biology, biogeography, ecology and conservation. The genus Paracaryum belongs to tribe Cynoglosseae of the family Boraginaceae is a herbaceous genus including approximately 67 species, mostly distributed in the Irano-Turanian phytogeographical region. In spite vast distribution of many Paracaryum species that grow in different habitats, there are not any available report on their genetic diversity, mode of divergence and patterns of dispersal. Therefore, we performed molecular (ISSR markers) of 98 accessions from 12 species of Paracaryum that were collected from different habitats. A set of 10 ISSR markers was used. The genetic distances were estimated based on Jaccard similarity coefficient and the descriptive statistics of populations for estimation of genetic parameters were also performed. A total of 90 polymorphic bands were obtained. The present study revealed that ISSR data can delimit the species. AMOVA and STRUCTURE analysis revealed that the species of Paracaryum belongs are genetically differentiated but have some degree of shared common alleles.

scholarly journals PENGARUH DOSIS PUPUK FOSFOR DAN KONSENTRASI GIBERELIN PADA PERTUMBUHAN DAN HASIL TANAMAN KUBIS BUNGA (Brassica oleraceea L.)

2017 ◽  
Vol 11 (2) ◽  
pp. 18-28
Author(s):  
Nur Rohman ◽  
Jeka Widiatmanta
Keyword(s):  
Agricultural Land ◽  
Block Design ◽  
Flowering Plant ◽  
Elemental Content ◽  
Phosphorus Fertilizer ◽  
Randomized Block Design ◽  
The Family ◽  
Flower Diameter ◽  
Main Plot ◽  
Number Of Leaves

The flower cabbage (Brassica oleraceea L.) is a type of vegetable plant belonging to the family of cabbage plants (Cruciferae). Flower cabbage often called "cauliflower" has many benefits to consume, because it contains calories, protein, carbohydrates, calcium, and vitamins A, B1 and C. Farming is very much affected by the state of agricultural land, a problem that often occurs due to lack of elemental content nutrients in the soil that affect the inflorescence and flower cabbage growth. Therefore, to increase the yield of cabbage flowers offered the method of land optimization with the addition of nutrients in the form of phosphorous soil and also the addition of gibberellin hormone that has a role to flowering cabbage plants. The research was conducted by using Randomized Block Design (RBD) in divided plots with 2 treatments, Phosphorus (P) as the main plot with 3 treatment levels P1 (100 kg / ha), P2 (125 kg / ha) and P3 150 kg/ha then the concentration of Giberelin (K) as a subplot with 3 levels of treatment K1 (75 mg / L), K2 (100 mg / L) and K3 (125 mg / L)). From the result of the combination of the two treatments were 9 treatment combinations, repeated 3 times to obtain 27 units combination of treatments, each experimental plot consisted of 15 plants. Parameters observed include plant height, number of leaves, number of flowering plants, flower diameter and flower weight. The result of the research showed no significant interaction in all plant variables but the dosage of phosphorus fertilizer gave significant effect on the height variable of the 10 hst plant on the P1 (100 kg/ha) treatment, the leaf number variant showed significant effect at age 40 hst on P3 treatment (150 kg / ha), the number of flowering plant variables showed significant effect of 60 hst on P3 treatment (150 kg / ha), flower diameter variables showed significant differences in P3 treatment (150 kg / ha). The best treatment is P3 treatment (150 kg / ha). Gibberellin concentrations did not have a real effect on all plant variants.

scholarly journals Analysis of Nepenthes Hybrids in Bukik Taratak West Sumatra by RAPD Technique

2018 ◽  
Vol 19 (2) ◽  
pp. 12-20
Author(s):  
Weni Enjelina ◽  
Mansyurdin Mansyurdin ◽  
Tesri Meideliza
Keyword(s):  
Similarity Coefficient ◽  
Family Tree ◽  
Natural Hybrid ◽  
Jaccard Similarity ◽  
Parental Relationship ◽  
Natural Hybrids ◽  
West Sumatra ◽  
Ctab Method ◽  
Relationship Of ◽  
The Relationship

The Study about Analysis of Nepenthes Hybrids in Taratak Hill, Pesisir Selatan Sumatera Barat by RAPD Technique had been conducted from May until November 2011. The study was done to prove natural hybrids of Nepenthes by RAPD technique and to determine the relationship of natural hybrids of Nepenthes with both parental. The research method is survey and observation. Isolation of DNA carried by CTAB method with modifications and amplification carried out by PCR. Hybrids and parental relationship was obtained from Jaccard similarity coefficient and displayed in a Dendogram (family tree) and PCO analysis to look genetic variation was processed with the program PAST 2.10. Proof of the natural hybrid N. ampullaria × N. mirabilis can be done by the RAPD technique using the primers OPA 15, OPK 16, OPK 19 and OPP 15. Natural hybrids between N. ampullaria × N. gracilis using the primers OPA 15, OPK 19 and OPP 15. The eighth of natural hybrids N. ampullaria × N. mirabilis were closer to N. mirabilis, while both natural hybrids N. ampullaria × N. gracilis closer to the N. Ampullaria.

scholarly journals IDENTIFIKASI KERAGAMAN GENETIK KENTANG SUPEJOHN TRANSGENIK (Solanum tuberosum L. var supejohn)

EUGENIA ◽  
2012 ◽  
Vol 18 (3) ◽  
Author(s):  
Christine L.W. Lengkong ◽  
Jeany Polii-Mandang ◽  
Edy F. Lengkong
Keyword(s):  
Genetic Diversity ◽  
Dna Isolation ◽  
Rapd Analysis ◽  
Solanum Tuberosum L ◽  
Similarity Coefficient ◽  
Agarose Gel ◽  
Buffer Solution ◽  
Random Primers ◽  
Average Similarity ◽  
Polymorphic Bands

ABSTRACT   The aim of this study was to determine the genetic diversity of potato Supejohn transgenic that had been twenty-five times subcultured with exploration  method. The genetic variability was calculated using molecular marker Polimorphic Random Amplified DNA (RAPD) analysis of ten samples from four weeks planlets of Supejohn transgenic plants using ten random primers. DNA isolation of 10 samples using CTAB buffer  then measurement of the DNA qualification and quantification,  afterwards amplification of DNA by PCR using 10 random primers followed by electrophoresis on a 1% agarose gel with TAE 1x electrode buffer solution. Visualisation  the results used UV Transluminator to see DNA bands and the data of  polymorphic bands had been analized used the NTSYS-pc version of 1.07 program to obtain the similarity coefficient  and the dendrogram.The results showed that five random primers produced polymorphic DNA bands with 0.39 - 0.88 similarity coefficient  and the average similarity coefficient is 0.63 (63%) or the genetic diversity of the samples as many as 37%. Dendrogram formed eight distinct clusters corresponding similarity coefficient, the formation of clusters means that there is genetic diversity among the DNA samples. Keywords: potato Supejohn transgenic, RAPD, genetic diversity ABSTRAK Penelitian ini bertujuan untuk  menentukan keragaman genetik kentang Supejohn transgenik yang telah dua puluh lima kali disubkultur dengan menggunakan metode penelitian eksplorasi dengan penanda molekuler  Random Amplified Polimorphic DNA (RAPD) terhadap 10 sampel tanaman Supejohn transgenik, berumur empat minggu dan  menggunakan  10 primer random.  Isolasi DNA dari 10 sampel menggunakan buffer CTAB sesudah itu dilakukan pengukuran kualitas dan kuantitas  DNA, kemudian dilanjutkan dengan amplifikasi DNA secara PCR menggunakan 10 primer random diikuti dengan  elektroforesis pada gel agarose 1% dengan larutan penyanggah elektroda TAE 1x. Visualisasi hasil elektroforesis  menggunakan UV Transluminator untuk melihat pita DNA  dan analisis  data pita polimorfik  menggunakan program NTSYS-pc versi 1.07 sehingga didapatkan koefisien kesamaan dan dendrogram. Hasil penelitian menunjukkan bahwa lima primer random menghasilkan pita DNA polimorfik dengan koefisien kesamaan 0.39 – 0.88 dan rata-rata koefisien kesamaan yaitu 0.63 (63%) atau  keragaman  genetik sampel sebesar 37%.  Dendrogram  membentuk delapan cluster yang berbeda sesuai koefisien kesamaan,terbentuknya clusters mengartikan bahwa ada keragaman genetik antar sampel DNA. Eugenia Volume 18 No. 3  Desember 2012 Kata kunci : kentang Supejohn transgenik , RAPD, keragaman genetik

scholarly journals Some preliminary phytochemical screening and assessment of four solvents extracts of button weed (Borreria verticillata)

2021 ◽  
Vol 24 (12) ◽  
pp. 2085-2088
Author(s):  
G.F. Kontagora ◽  
N. Lawal ◽  
D.A. Adebote ◽  
B. Kamba ◽  
M.I. Nafiu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Phytochemical Screening ◽  
Woody Perennial ◽  
Whole Plant ◽  
The Family ◽  
The World ◽  
Perennial Shrub ◽  
Ancient Times ◽  
Ethyl Acetate Extracts

Borreria vertcillata is a woody perennial shrub with false-button weedy herb belonging to the family (Rubiaceae), used for treating/curing various forms of diseases across the world since ancient times. Qualitative phytochemical screening of Borreria vertcillata pulverized whole plant was carried out using four different solvent extracts (N-hexane, chloroform, ethyl-acetate and methanol). The phytochemicals screeened contained alkaloids, triterpenes, flavonoids, glycosides, tannins, saponnins, anthraquinones and steroids. The extracts were then screened for the presence of some phytochemicals such as alkaloids, anthraquinones, saponnins, steroids, terpenes, flavonoids, tannins and glycosides. All the extracts contained alkaloids, triterpenes and glycosides present but flavonoids, saponnins and tannins only present in ethyl acetate and methanol while anthraquinones and steroids were totally absent from the extracts .Of all the extracts Ethyl acetate extracts had the most influential effects on pathogenic organisms such as Culex quinquefasciatus, Staphylococcus aureus, Escherichia coli and Candidas albican

scholarly journals An ISSR-based genetic diversity analysis of Malus sieversii in Tienshan Mountains in Xinjiang, China and Kyrgyzstan

2019 ◽  
pp. 40-48
Author(s):  
Meiling Yang ◽  
Shiyou Che ◽  
Hong Long ◽  
Guorong Yan ◽  
Weiwei Yu
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Inter Simple Sequence Repeat ◽  
Similarity Coefficient ◽  
Malus Sieversii ◽  
Long Time ◽  
Polymorphism Rate ◽  
Different Populations ◽  
Xinjiang Of China ◽  
Issr Primers

Malus sieversii constitute a valuable genetic resource in wild apple ecosystems. The aim of this study was to use inter-simple sequence repeat (ISSR) primers as an accessible tool to investigate the genetic diversity in Malus sieversii species. The experimental materials include 34 samples from Kyrgyzstan and Xinjiang of China. A total of 125 bands and 98 polymorphic bands were amplified using 47 ISSR primers. The polymorphism rate was 78.4%. The genetic similarity coefficient of Kyrgyzstan and Xinjiang of China population was 0.68; the genetic similarity coefficient of various populations in Xinjiang was 0.72~0.94. The samples in same population got into a category, but some samples in faraway geographic locations have cross clustering. Geographical isolation hindered the gene exchange of Malus sieversii in different populations for a long time, and Malus sieversii developed along the natural selection environment direction and generate genetic differentiation after that.

scholarly journals PENGARUH DOSIS PUPUK FOSFOR DAN KONSENTRASI GIBERELIN PADA PERTUMBUHAN DAN HASIL TANAMAN KUBIS BUNGA (Brassica oleraceea L.)

2017 ◽  
Vol 11 (2) ◽  
pp. 18-28
Author(s):  
Nur Rohman ◽  
Jeka Widiatmanta
Keyword(s):  
Agricultural Land ◽  
Block Design ◽  
Flowering Plant ◽  
Elemental Content ◽  
Phosphorus Fertilizer ◽  
Randomized Block Design ◽  
The Family ◽  
Flower Diameter ◽  
Main Plot ◽  
Number Of Leaves

The flower cabbage (Brassica oleraceea L.) is a type of vegetable plant belonging to the family of cabbage plants (Cruciferae). Flower cabbage often called "cauliflower" has many benefits to consume, because it contains calories, protein, carbohydrates, calcium, and vitamins A, B1 and C. Farming is very much affected by the state of agricultural land, a problem that often occurs due to lack of elemental content nutrients in the soil that affect the inflorescence and flower cabbage growth. Therefore, to increase the yield of cabbage flowers offered the method of land optimization with the addition of nutrients in the form of phosphorous soil and also the addition of gibberellin hormone that has a role to flowering cabbage plants. The research was conducted by using Randomized Block Design (RBD) in divided plots with 2 treatments, Phosphorus (P) as the main plot with 3 treatment levels P1 (100 kg / ha), P2 (125 kg / ha) and P3 150 kg/ha then the concentration of Giberelin (K) as a subplot with 3 levels of treatment K1 (75 mg / L), K2 (100 mg / L) and K3 (125 mg / L)). From the result of the combination of the two treatments were 9 treatment combinations, repeated 3 times to obtain 27 units combination of treatments, each experimental plot consisted of 15 plants. Parameters observed include plant height, number of leaves, number of flowering plants, flower diameter and flower weight. The result of the research showed no significant interaction in all plant variables but the dosage of phosphorus fertilizer gave significant effect on the height variable of the 10 hst plant on the P1 (100 kg/ha) treatment, the leaf number variant showed significant effect at age 40 hst on P3 treatment (150 kg / ha), the number of flowering plant variables showed significant effect of 60 hst on P3 treatment (150 kg / ha), flower diameter variables showed significant differences in P3 treatment (150 kg / ha). The best treatment is P3 treatment (150 kg / ha). Gibberellin concentrations did not have a real effect on all plant variants.

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