scholarly journals The Effectivity of Enterotoxigenic Escherichia coli (Multivalen) Vaccination in Swine Case Study in Bali

2021 ◽  
pp. 138-143
Author(s):  
Anastasia Sischa Jati Utami ◽  
Ida Ayu Parwati ◽  
I Nyoman Suyasa
Keyword(s):  
Escherichia Coli ◽  
Block Design ◽  
Enterotoxigenic Escherichia Coli ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Randomized Block Design ◽  
Postnatal Mortality ◽  
Vaccine Treatment ◽  
Elisa Technique

Swine cattle have high economic advantages and social values ​​ in Balinese society. This study aimed to determine the effectiveness of Enterotoxigenic Escherichia coli (ETEC) vaccine in gestating sows. We used 26 gestating sows aged 1.5 years old at their last trimester assigning 13 sows in the control group and 13 sows in treatment group to receive multivalent ETEC vaccine twice: on the 70-75 days of gestation and a booster on 100-105 days of gestation. Sows with the vaccine treatment divided into 3 group that groups A was treated antifimbrial K41, groups B was antifimbrial 987P, while in group C was Antiimbrial K88. The changes in temperature were observed from one day before to 4 days after vaccination in a randomized block design. To determine the antibody formed, blood samples were taken one and two weeks after the first vaccine and five weeks after the booster. The blood sample was analyzed by the Enzyme Linked Immunosorbent Assay (ELISA) technique. The rates of diarrhea and postnatal mortality were also observed. The results showed that the antibody titer level increased significantly in the fifth week in groups A with titer 1,94 ±0,22 and B with titer 1,98 ± 0,30 , while the number of diarrhea incidents was 0.9% compared to control 34% with a mortality rate of 0%. In conclusion, this multivalent ETEC vaccine is safe to use and has been shown to be effective for ETEC cases in swine.

scholarly journals Advertising Personality Impact to Education Institution Brand Image Base on Three Hits Theory of Exposure

2015 ◽  
Vol 21 (4) ◽  
pp. 777-780
Author(s):  
Rudy Aryanto ◽  
Sisca Apriliyanti ◽  
Bachtiar H. Simamora ◽  
Idris Gautama So
Keyword(s):  
Brand Image ◽  
Block Design ◽  
Education Institution ◽  
Control Group ◽  
Randomized Block Design ◽  
Print Advertisement ◽  
Information And Communication ◽  
And Control ◽  
One Way Anova

This research conducted to examine the causal effects of two dimensional design of print advertisement communication personality in tutoring course education institution base on brand personality that beneficially applied toward tutoring education institution brand image. After conducting observation of three groups of participants with different information advertisement treatment i.e., sincerity personality, excitement personality and control group, in the longitudinal three weeks experiment using One-Way ANOVA randomized block design analysis, gathered in the context of case study. It is proven that sincerity advertisement treatment is not appropriate to apply in making information and advertisement design for this case if publish more than one time broadcast. Contrarily, for the excitement personality form of advertisement treatment appear to increase influence on the brand image aggregate for three weeks, means excitement personality design of advertisement will strengthened the three hits theory. These research outcomes, when applied to education institution will providers, will help in designing appropriate personality form of information and communication of advertisement.

scholarly journals Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Femi Ayoade ◽  
Judith Oguzie ◽  
Philomena Eromon ◽  
Omolola E. Omotosho ◽  
Tosin Ogunbiyi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Block Design ◽  
Latex Agglutination ◽  
Pcr Analysis ◽  
Accession Number ◽  
E Coli ◽  
Representative Isolate ◽  
Randomized Block Design ◽  
Toxigenic Strains

AbstractShiga toxigenic strains of E. coli (STEC) known to be etiological agents for diarrhea were screened for their incidence/occurrence in selected abattoirs sources in Osogbo metropolis of Osun State, Nigeria using a randomized block design. Samples were plated directly on selective and differential media and E. coli isolates. Multiplex PCR analysis was used to screen for the presence of specific virulence factors. These were confirmed serologically as non-O157 STEC using latex agglutination serotyping kit. Sequence analysis of PCR products was performed on a representative isolate showing the highest combination of virulence genes using the 16S gene for identification purposes only. Results showed that the average cfu/cm2 was significantly lower in the samples collected at Sekona-2 slaughter slab compared with those collected at Al-maleek batch abattoir and Sekona-1 slaughter slab in ascending order at P = 0.03. Moreover, the average cfu/cm2E. coli in samples collected from butchering knife was significantly lower when compared with that of the workers’ hand (P = 0.047) and slaughtering floor (P = 0.047) but not with the slaughter table (P = 0.98) and effluent water from the abattoir house (P = 0.39). These data suggest that the abattoir type may not be as important in the prevalence and spread of STEC as the hygiene practices of the workers. Sequence analysis of a representative isolate showed 100% coverage and 96.46% percentage identity with Escherichia coli O113:H21 (GenBank Accession number: CP031892.1) strain from Canada. This sequence was subsequently submitted to GenBank with accession number MW463885. From evolutionary analyses, the strain from Nigeria, sequenced in this study, is evolutionarily distant when compared with the publicly available sequences from Nigeria. Although no case of E. coli O157 was found within the study area, percent occurrence of non-O157 STEC as high as 46.3% at some of the sampled sites is worrisome and requires regulatory interventions in ensuring hygienic practices at the abattoirs within the study area.

scholarly journals Interference of Urochloa plantaginea on Morphophysiology and Yield Components of Black Beans

2019 ◽  
Vol 11 (9) ◽  
pp. 272
Author(s):  
Milena Barretta Franceschetti ◽  
Leandro Galon ◽  
Maico André Michelon Bagnara ◽  
Renan Pawelkiewicz ◽  
Leonardo Brunetto ◽  
...  
Keyword(s):  
Block Design ◽  
Plant Morphology ◽  
Co2 Concentration ◽  
Crop Productivity ◽  
Dry Mass ◽  
Control Group ◽  
Total Period ◽  
Black Beans ◽  
Physiological Variables ◽  
Randomized Block Design

Among the factors which affect crop productivity, there are weeds which compete for environment resources. The objective of this work was to determine the periods of interference of alexandergrass on the morphophysiology and productivity components of black beans. The experiment was conducted in a complete randomized block design with four replications. The bean cultivar of the black type used was IPR Uirapuru, being the treatments separated in two models of interference: in the coexistence group, the crop of the bean lived with the alexandergrass by growing periods of 0, 7, 14, 21, 28, 35, 42 and throughout the cycledays after emergence and throughout the cycle; in the control group the crop was kept free of the infestation for the same periods described previously. At 42 DAE were evaluated the variables related to plant morphology and physiology (height of bean plants, number of trifoliate leaves, dry mass plant, number of grains pods, number od pods plants, stomatal conductance, internal CO2 concentration, photosynthetic activity, efficiency of carboxylation, efficient use of water, transpiration rate). Considering the results, it is possible to conclude that the critical period of interference prevention (CPIP) comprises 24 to 50 DAE, the period before the interference (PBI) was 24 DAE and the total period of interference prevention (TPIP) was 50 days and that the morphological and physiological variables of the plants were negatively affected in the coexistence with the alexandergrass when compared to the treatments kept free of weeds throughout the crop cycle.

scholarly journals PERBEDAAN PENURUNAN TEKANAN DARAH PRA LANSIA PRE HIPERTENSI YANG DIBERI JUS TOMAT (Lycopersicum grandifilum)

2018 ◽  
Vol 10 (2) ◽  
pp. 152-158
Author(s):  
Hari Yani Fadillah
Keyword(s):  
Blood Pressure ◽  
Treatment Group ◽  
Block Design ◽  
Pressure Control ◽  
Tomato Juice ◽  
Control Group ◽  
Randomized Block Design ◽  
Before And After ◽  
Blood Pressure Treatment ◽  
And Control

The research aims to know the effect of tomato juice on blood pressure in pre-elderly pre hypertension at Posbindu Puskesmas Nusa Indah Bengkulu City.The design of research was a quasi experiment include pre test post test. This population was all patients posbindu aged 45-59 years. Sampling of this research randomized block design of one factorial were 6 samples divided into treatment and control group. The data which have been analyzed using univariate and bivariate. From this research were obtained the results such as the mean of sistole blood pressure before and after intervention in treatment group p = 0,273 and diastole p = 0,27. Mean systole blood pressure control group p = 0.060 and diastole p = 2.14. Distribution of tomato juice (Lycopersicum grandifilum) did not given affect the decrease in blood pressure of systole diastole before and after intervention in pre-elderly pre hypertension. Whereas the difference in mean systole of blood pressure treatment and control group p = 0.935 and diastolic of blood pressure p = 0.678. There ware not difference in mean of blood pressure in the tomato juice treatment group and control of Vitamin C. The result concluded there were not effect of tomato juice (Lycopersicum grandifilum) on systolic of blood pressure in pre-elderly pre hypertension at Posbindu Puskesmas Nusa Indah Bengkulu City.

scholarly journals The Effect of Writing Therapy on Anxiety in Pregnant Women: A Randomized Controlled Trial

2020 ◽  
Vol 14 (2) ◽  
Author(s):  
Maryam Montazeri ◽  
Khalil Esmaeilpour ◽  
Sakineh Mohammad-Alizadeh-Charandabi ◽  
Sevda Golizadeh ◽  
Mojgan Mirghafourvand
Keyword(s):  
Randomized Controlled Trial ◽  
Pregnant Women ◽  
Controlled Trial ◽  
Block Design ◽  
Intervention Group ◽  
Control Group ◽  
Randomized Controlled ◽  
Writing Therapy ◽  
Randomized Block Design ◽  
Significant Difference

Background: As one of the most enjoyable life experiences, pregnancy may be accompanied by many physiological and psychological changes that make women susceptible to developing mental disorders such as anxiety. Non-pharmacological methods such as writing therapy are among the ways to deal with anxiety. Objectives: The present study aimed to determine the effect of writing therapy on anxiety in women during pregnancy. Methods: This randomized controlled trial was conducted on 70 pregnant women with a gestational age between 28 - 31 weeks. The participants were assigned to an intervention group and a control group using a randomized block design. The intervention group received three in-person writing therapy-based counseling sessions and two telephone calls between the sessions, while the control group received routine pregnancy care. The Beck anxiety inventory was completed before and six weeks after the intervention. Independent t-test and ANCOVA were used for data analysis. Results: There was no significant difference between the two groups in terms of socio-demographic variables and baseline anxiety scores. Based on ANCOVA with baseline score adjustment, the mean score of anxiety was significantly lower in the intervention group than in the control group after the intervention (adjusted mean difference = -6.8; 95%CI = -4.5 to -9.1; P < 0.001). Conclusions: According to the results, writing therapy can reduce anxiety in pregnant women; however, further studies are needed before a definitive conclusion.

478 Dietary Butyrate and Valerate Glycerides: Effects on Growth and Systemic Immunity of Weanling Piglets Coinfected with F4 and F18 Escherichia coli

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 211-212
Author(s):  
Lauren L Kovanda ◽  
Jungjae Park ◽  
Yijie He ◽  
Sangwoo Park ◽  
Ruochen Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Block Design ◽  
Enterotoxigenic Escherichia Coli ◽  
Post Inoculation ◽  
Weaned Pigs ◽  
Fecal Shedding ◽  
Systemic Immunity ◽  
Randomized Complete Block Design ◽  
Potential Benefits ◽  
Weanling Piglets

Abstract Enterotoxigenic Escherichia coli (ETEC) F4 and F18 are the two most dominant pathogenic strains in weaned pigs. The objective of this experiment was to test the effects of dietary monobutyrin and monovalerin on performance and systemic immunity of weanling piglets coinfected with F4/F18 ETEC. Twenty weaned pigs (8.21 ± 1.23 kg) were individually housed and were randomly allotted to one of three diets: control (n = 6), 0.1% monobutyrin (n = 7), or 0.1% monovalerin (n = 7). The experiment was conducted 14 days, including 7 days’ adaption and 7 days post-inoculation (PI). On d 0, d 1, and d 2 PI, pigs were inoculated with 0.5 × 109 CFU/1.5 mL each of F4 and F18 ETEC for three consecutive days. Diarrhea score was recorded daily to determine frequency of diarrhea. Piglets and feeders were weighed throughout the trial to analyze growth performance. Fecal cultures from pigs on d 0, 2, and 4 PI were inspected to identify the absence or presence of hemolytic coliforms. Blood was collected on d 0, 4, and 7 PI for complete blood cells count. All data were analyzed by the Proc Mixed of SAS with randomized complete block design. Pigs supplemented with monovalerin and monobutyrin had numerically higher ADG (249 and 282 g/day) from d 0 to d 7 PI than pigs in control (198 g/day). Supplementation of monovalerin reduced (P &lt; 0.05) frequency of diarrhea throughout the experiment. Pigs fed monovalerin had lower (P &lt; 0.05) neutrophil counts on d 4 PI compared with control. Hemolytic coliforms were observed in all fecal cultures from d 2 and d 4 PI, confirming fecal shedding of ETEC. Results of this study indicate the potential benefits of monovalerin supplementation on performance and disease resistance of weaned pigs coinfected with F4 and F8 ETEC.

scholarly journals Antibodies Change the Mechanics of Adhesion Fimbriae - a Case Study of CS20 Fimbriae Expressed by Enterotoxigenic Escherichia Coli

2015 ◽  
Vol 108 (2) ◽  
pp. 602a
Author(s):  
Narges Mortezaei ◽  
Bhupender Singh ◽  
Bernt Eric Uhlin ◽  
Stephen J. Savarino ◽  
Esther Bullitt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli

Detection of enterotoxigenic Escherichia coli in water by polymerase chain reaction amplification and hybridization

1994 ◽  
Vol 40 (4) ◽  
pp. 243-249 ◽  
Author(s):  
Z. Tamanai-Shacoori ◽  
A. Jolivet-Gougeon ◽  
M. Pommepuy ◽  
M. Cormier ◽  
R. R. Colwell
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Amplification ◽  
Pcr Amplification ◽  
Environmental Water ◽  
Latex Agglutination ◽  
Enterotoxigenic Escherichia Coli ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Polymerase Chain

Enterotoxigenic Escherichia coli was studied in waste water, river water, and seawater from six locations along the west coast of Normandy by using the polymerase chain reaction (PCR) to amplify the heat labile (LT) gene. Cellular DNA was extracted from centrifugation pellets and amplified using PCR. The PCR products were detected by gel electrophoresis and confirmed by hybridization assay, using an 850 base pair HindIII DNA fragment probe from pEWD299 conjugated to digoxigenin and specific for the LT gene. Results of the PCR amplification were compared with those of GM1 enzyme-linked immunosorbent assay, latex agglutination, and colony hybridization. The PCR method was found to be more precise and less time consuming, especially when compared with methods requiring culture of isolates for enumeration of enterotoxigenic E. coli in water.Key words: enterotoxigenic Escherichia coli, PCR, environmental water, digoxigenin.

scholarly journals SIRKULASI KAPILER KAKI PASIEN DIABETES MELLITUS TIPE-2 MELALUI RENDAM KAKI DENGAN MINYAK TANAH GARAM DINGIN DI PUSKESMAS BAITUSSALAM KABUPATEN ACEH BESAR

2020 ◽  
Vol 7 (1) ◽  
pp. 41-51
Author(s):  
Setio Budi Raharjo ◽  
Amiruddin Amiruddin
Keyword(s):  
Type 2 Diabetes ◽  
Random Sampling ◽  
Design Research ◽  
Block Design ◽  
Ankle Brachial Index ◽  
Peripheral Circulation ◽  
Control Group ◽  
Type 2 Dm ◽  
Randomized Block Design

Sirkuasi perifer kaki pasien DM Tipe 2 sangat tergantung pada nilai ankle brachial index (ABI). Untuk mengoptimalkan nilai ABI dalam batas normal, maka peneliti memberikan terapi non farmakalogi. Implementasi non farmakologi ini membantu meningkatkan sirkulasi perifer kaki dan bahan yang digunakan dalam penelitian ini adalah minyak tanah, garam dapur dan es batu.   Penelitian menggunakan randomized block design research, terdiri dari 4 perlakuan dengan masing-masing perlakuan pada kelompok: A= hanya diberi minyak tanah (1 liter) sebagai kelompok kontrol; B=diberi minyak tanah 1 liter: 500 gram garam: 500 gram es batu; C= diberi minyak tanah 1 liter: 750 gram garam: 750 gram es batu; dan D= diberi minyak tanah 1 liter: 1 Kg garam: 1 Kg es batu. Random sampling dilakukan dengan komputer. Hasil  penelitian  diperoleh  bahwa intervensi rendam kaki minyak tanah garam dingin yang memberikan dampak nyata pada nilai ABI pasien DM Tipe 2, yaitu perlakuan C yaitu rendam kaki dengan larutan 1 liter tanah: 750 gram garam: dan 750 gram es batu. Komposisi larutan yang paling berpengaruh terhadap perubahan nilai ABI pasien DM Tipe 2 adalah 1 liter minyak tanah: 750 gram garam: 750 gram es batu. Kesimpulan, penelitian ini telah menunjukkan bahwa sirkulasi perifer kaki pasien DM Tipe 2 dapat ditingkatkan melalui rendam kaki dengan minyak tanah garam dingin. Circulation of the peripheral feet of patients with Type 2 diabetes is very dependent on the value of the ankle brachial index (ABI). To optimize the ABI value within normal limits, the researchers provided nonpharmacological therapy. This nonpharmacological implementation helps improve the peripheral circulation of the feet and the materials used in this study are kerosene, table salt and ice cubes. The research used randomized block design research, consisting of 4 treatments with each treatment in the group: A = only given kerosene (1 liter) as a control group; B = oiled with 1 liter of soil: 500 grams of salt: 500 grams of ice cubes; C = oiled with 1 liter of soil: 750 grams of salt: 750 grams of ice cubes; and D = oiled with 1 liter of soil: 1 kg of salt: 1 kg of ice cubes. Random sampling is done by computer. The results showed that the cold salt kerosene foot soaking intervention had a significant impact on the ABI value of Type 2 DM patients, namely treatment C, namely soaking the feet with a solution of 1 liter of soil: 750 grams of salt: and 750 grams of ice cubes. The composition of the solution that most influences changes in the ABI value of Type 2 DM patients is 1 liter of kerosene: 750 grams of salt: 750 grams of ice cubes. Conclusion, this study has shown that the peripheral circulation of the feet of patients with type 2 diabetes can be improved by soaking the feet with cold salt kerosene.

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